Li Rong Yu

Summary

Affiliation: National Cancer Institute
Country: USA

Publications

  1. ncbi request reprint Proteomic analysis of detergent-resistant membrane rafts
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, National Cancer Institute, MD, USA
    Electrophoresis 25:1307-18. 2004
  2. pmc Quantitative proteomic analysis of human breast epithelial cells with differential telomere length
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Biochem Biophys Res Commun 356:942-7. 2007
  3. doi request reprint Phosphoproteomics for the discovery of kinases as cancer biomarkers and drug targets
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD, USA
    Proteomics Clin Appl 1:1042-57. 2007
  4. ncbi request reprint Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, P O Box B, Frederick, Maryland 21702, USA
    J Proteome Res 6:4150-62. 2007
  5. ncbi request reprint AACR-FDA-NCI cancer biomarkers collaborative
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    Expert Rev Mol Diagn 7:507-9. 2007
  6. ncbi request reprint Quantitative profiling of the detergent-resistant membrane proteome of iota-b toxin induced vero cells
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland 21702 1201, USA
    J Proteome Res 4:523-31. 2005
  7. ncbi request reprint A detergent- and cyanogen bromide-free method for integral membrane proteomics: application to Halobacterium purple membranes and the human epidermal membrane proteome
    Josip Blonder
    SAIC Frederick Inc, Laboratory of Proteomics and Analytical Technologies Mass Spectrometry Center, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    Proteomics 4:31-45. 2004
  8. ncbi request reprint A combined proteome and microarray investigation of inorganic phosphate-induced pre-osteoblast cells
    Kelly A Conrads
    Laboratory of Cancer Prevention, Center for Cancer Research, SAIC Frederick, Inc, National Cancer Institute, Frederick, MD 21702, USA
    Mol Cell Proteomics 4:1284-96. 2005
  9. ncbi request reprint Analysis of murine natural killer cell microsomal proteins using two-dimensional liquid chromatography coupled to tandem electrospray ionization mass spectrometry
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, Frederick, Maryland 21702 1201, USA
    J Proteome Res 3:862-70. 2004
  10. ncbi request reprint Evaluation of the acid-cleavable isotope-coded affinity tag reagents: application to camptothecin-treated cortical neurons
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland 21702 1201, USA
    J Proteome Res 3:469-77. 2004

Collaborators

Detail Information

Publications32

  1. ncbi request reprint Proteomic analysis of detergent-resistant membrane rafts
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, National Cancer Institute, MD, USA
    Electrophoresis 25:1307-18. 2004
    ..A total of 91 (24%) proteins were classified as integral alpha-helical membrane proteins, of which 51 (56%) were predicted to have multiple transmembrane domains...
  2. pmc Quantitative proteomic analysis of human breast epithelial cells with differential telomere length
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Biochem Biophys Res Commun 356:942-7. 2007
    ..Interestingly, the DNA excision repair pathway is enhanced, while integrin and its ligands are downregulated in the cells with long telomeres. These results may provide valuable information related to telomere functions...
  3. doi request reprint Phosphoproteomics for the discovery of kinases as cancer biomarkers and drug targets
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD, USA
    Proteomics Clin Appl 1:1042-57. 2007
    ..Strategies for global, targeted, and quantitative phosphoproteomics are discussed, and some recent interesting applications are also evaluated...
  4. ncbi request reprint Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, P O Box B, Frederick, Maryland 21702, USA
    J Proteome Res 6:4150-62. 2007
    ..The study also indicates that evaluation of confidence levels for phosphopeptide identification via the reversed sequence database searching strategy might underestimate the false positive rate...
  5. ncbi request reprint AACR-FDA-NCI cancer biomarkers collaborative
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    Expert Rev Mol Diagn 7:507-9. 2007
    ....
  6. ncbi request reprint Quantitative profiling of the detergent-resistant membrane proteome of iota-b toxin induced vero cells
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland 21702 1201, USA
    J Proteome Res 4:523-31. 2005
    ..The quantitative profiling of Ib-induced DRMP from Vero cells revealed several proteins with altered expression levels suggesting their possible role in Ib binding/uptake...
  7. ncbi request reprint A detergent- and cyanogen bromide-free method for integral membrane proteomics: application to Halobacterium purple membranes and the human epidermal membrane proteome
    Josip Blonder
    SAIC Frederick Inc, Laboratory of Proteomics and Analytical Technologies Mass Spectrometry Center, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    Proteomics 4:31-45. 2004
    ....
  8. ncbi request reprint A combined proteome and microarray investigation of inorganic phosphate-induced pre-osteoblast cells
    Kelly A Conrads
    Laboratory of Cancer Prevention, Center for Cancer Research, SAIC Frederick, Inc, National Cancer Institute, Frederick, MD 21702, USA
    Mol Cell Proteomics 4:1284-96. 2005
    ..The ability to accurately measure changes in both protein abundance and mRNA levels on a system-wide scale represents a novel means to extract data from previously one-dimensional datasets...
  9. ncbi request reprint Analysis of murine natural killer cell microsomal proteins using two-dimensional liquid chromatography coupled to tandem electrospray ionization mass spectrometry
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, Frederick, Maryland 21702 1201, USA
    J Proteome Res 3:862-70. 2004
    ..These peptides resulted in identification of 2563 proteins of which 876 (34%) are classified as membrane proteins...
  10. ncbi request reprint Evaluation of the acid-cleavable isotope-coded affinity tag reagents: application to camptothecin-treated cortical neurons
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland 21702 1201, USA
    J Proteome Res 3:469-77. 2004
    ..The new ICAT reagents were then applied to the analysis of a cortical neuron proteome sample to identify proteins regulated by the antitumor drug, camptothecin...
  11. ncbi request reprint A proteomic characterization of the plasma membrane of human epidermis by high-throughput mass spectrometry
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, Mass Spectrometry Center, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland, USA
    J Invest Dermatol 123:691-9. 2004
    ..Furthermore, 121 proteins from cholesterol-rich plasma membrane domains (caveolar and lipid rafts) were identified...
  12. ncbi request reprint Global analysis of the cortical neuron proteome
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, MD 21702 1201, USA
    Mol Cell Proteomics 3:896-907. 2004
    ..The results presented here provide the broadest proteome coverage for a mammalian cell to date and show that MS-based proteomics has the potential to provide high coverage of the proteins expressed within a cell...
  13. ncbi request reprint Quantitative proteomic analysis of inorganic phosphate-induced murine MC3T3-E1 osteoblast cells
    Kelly A Conrads
    Laboratory of Cancer Prevention, Center for Cancer Research, Fredrick, MD, USA
    Electrophoresis 25:1342-52. 2004
    ..41-fold increase as determined by Western blotting. These results demonstrate that quantitative proteomics is capable of providing a quantitative view of thousands of proteins in mammalian cells within a defined set of experiments...
  14. ncbi request reprint On-column sample enrichment for capillary electrophoresis sheathless electrospray ionization mass spectrometry: evaluation for peptide analysis and protein identification
    George M Janini
    Laboratory of Proteomics and Analytical Technologies and Image Analysis Laboratory, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland 21702, USA
    Anal Chem 75:5984-93. 2003
    ..Testing of this novel CE-MS system showed its ability to characterize proteomic samples such as protein digests, in-gel-digested proteins, and hydrophobic peptides as well as to quantitate ICAT-labeled peptides...
  15. pmc Combined chemical and enzymatic stable isotope labeling for quantitative profiling of detergent-insoluble membrane proteins isolated using Triton X-100 and Brij-96
    Josip Blonder
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland 21702 1201, USA
    J Proteome Res 5:349-60. 2006
    ..This result confirms previous, targeted studies suggesting that DRMMs isolated using Triton X-100 and Brij-96 differ in their protein content...
  16. ncbi request reprint Diagnostic proteomics: serum proteomic patterns for the detection of early stage cancers
    Li Rong Yu
    Laboratory of Proteomics and Analytical Technologies, SAIC Frederick Inc, National Cancer Institute at Frederick, P O Box B, Frederick, MD 21702, USA
    Dis Markers 19:209-18. 2003
    ....
  17. ncbi request reprint Pooled ORF expression technology (POET): using proteomics to screen pools of open reading frames for protein expression
    William K Gillette
    Protein Expression Laboratory, Research Technology Program, SAIC Frederick, Inc NCI, National Institutes of Health, Frederick, Maryland 21702, USA
    Mol Cell Proteomics 4:1647-52. 2005
    ..Using POET, pools of ORFs can be constructed, and the pools of the resulting proteins can be analyzed and manipulated to rapidly acquire information about the attributes of hundreds of proteins simultaneously...
  18. pmc Identification of a novel Wnt5a-CK1ɛ-Dvl2-Plk1-mediated primary cilia disassembly pathway
    Kyung Ho Lee
    Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA
    EMBO J 31:3104-17. 2012
    ..This study may provide new insights into the mechanism underlying ciliary disassembly processes and various cilia-related disorders...
  19. ncbi request reprint Proteomic analysis by two-dimensional polyacrylamide gel electrophoresis
    Ming Zhou
    SAIC Frederick, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA
    Adv Protein Chem 65:57-84. 2003
  20. pmc Increased oxidative-modifications of cytosolic proteins in 3,4-methylenedioxymethamphetamine (MDMA, ecstasy)-exposed rat liver
    Vijay V Upreti
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA
    Proteomics 11:202-11. 2011
    ..Our results show for the first time that MDMA induces oxidative-modification of many cytosolic proteins accompanied with increased oxidative stress and apoptosis, contributing to hepatic damage...
  21. ncbi request reprint Proteome analysis of DNA damage-induced neuronal death using high throughput mass spectrometry
    Mark D Johnson
    Department of Neurological Surgery, University of Washington School of Medicine, Seattle, Washington 98195 6470, USA
    J Biol Chem 279:26685-97. 2004
    ..These data demonstrate the power of high throughput mass spectrometry for quantitative analysis of the neuronal proteome...
  22. ncbi request reprint Proteome analysis of camptothecin-treated cortical neurons using isotope-coded affinity tags
    Li Rong Yu
    Pacific Northwest National Laboratory, Richland, WA, USA
    Electrophoresis 23:1591-8. 2002
    ..While proteins from most functional classes of proteins were identified, a particularly large percentage of the enzymes involved in glycolysis and the tricarboxylic acid cycle were observed...
  23. ncbi request reprint Detection of in situ labeled cell surface proteins by mass spectrometry: application to the membrane subproteome of human mammary epithelial cells
    Wan Nan U Chen
    Cell Biology, Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA
    Proteomics 3:1647-51. 2003
    ..The method reported can be adapted to include stable isotope labeling of proteins for quantitation of changes occurring on the cell surface in response to specific perturbations...
  24. ncbi request reprint Phosphoprotein isotope-coded solid-phase tag approach for enrichment and quantitative analysis of phosphopeptides from complex mixtures
    Wei Jun Qian
    Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P O Box 999, MSIN K8 98, Richland, Washington 99352, USA
    Anal Chem 75:5441-50. 2003
    ..Its utility for proteomic applications was demonstrated by the labeling of soluble phosphoproteins from a human breast cancer cell line...
  25. ncbi request reprint Multidimensional proteome analysis of human mammary epithelial cells
    Jon M Jacobs
    Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99352, USA
    J Proteome Res 3:68-75. 2004
    ....
  26. ncbi request reprint Profiling the activity of G proteins in patient-derived tissues by rapid affinity-capture of signal transduction proteins (GRASP)
    David M Berman
    Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proteomics 4:812-8. 2004
    ..GRASP represents an advance in the field of proteomics as it detects protein interactions present in cells as they exist in their native tissue microenvironment...
  27. ncbi request reprint The proteomics of neurodegeneration
    Mark D Johnson
    Department of Neurological Surgery, University of Washington School of Medicine, Seattle, Washington 98195 6470, USA
    Am J Pharmacogenomics 5:259-70. 2005
    ....
  28. ncbi request reprint Self-regulated Plk1 recruitment to kinetochores by the Plk1-PBIP1 interaction is critical for proper chromosome segregation
    Young H Kang
    Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    Mol Cell 24:409-22. 2006
    ..Thus, Plk1 self-regulates the Plk1-PBIP1 interaction to timely localize to the kinetochores and promote proper chromosome segregation...
  29. ncbi request reprint Regulation of androgen receptor activity by tyrosine phosphorylation
    Zhiyong Guo
    Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Cancer Cell 10:309-19. 2006
    ....
  30. ncbi request reprint Direct phosphorylation and activation of a Nim1-related kinase Gin4 by Elm1 in budding yeast
    Satoshi Asano
    Laboratory of Metabolism, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 281:27090-8. 2006
    ..Thus, Elm1 regulates the septin assembly-dependent cellular events by directly phosphorylating and activating the Gin4-dependent pathway(s)...
  31. pmc Parallel analysis of transcript and translation profiles: identification of metastasis-related signal pathways differentially regulated by drug and genetic modifications
    Haiyan Yang
    Laboratory of Population Genetics, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    J Proteome Res 5:1555-67. 2006
    ....
  32. ncbi request reprint Quantitative proteomic analysis of sokotrasterol sulfate-stimulated primary human endothelial cells
    Aly Karsan
    Department of Medical Biophysics, British Columbia Cancer Agency, Vancouver, British Columbia V5Z 1L3, Canada
    Mol Cell Proteomics 4:191-204. 2005
    ..These findings are consistent with a role for sokotrasterol sulfate in endothelial sprouting...