Research Topics
Genomes and Genes | Li Rong YuSummaryAffiliation: National Cancer Institute Country: USA Publications
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Detail Information
Publications
Proteomic analysis of detergent-resistant membrane raftsJosip Blonder
Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick, National Cancer Institute, MD, USA
Electrophoresis 25:1307-18. 2004..A total of 91 (24%) proteins were classified as integral alpha-helical membrane proteins, of which 51 (56%) were predicted to have multiple transmembrane domains...- AACR-FDA-NCI cancer biomarkers collaborativeLi Rong Yu
Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
Expert Rev Mol Diagn 7:507-9. 2007.... 
Quantitative proteomic analysis of human breast epithelial cells with differential telomere lengthLi Rong Yu
Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
Biochem Biophys Res Commun 356:942-7. 2007..Interestingly, the DNA excision repair pathway is enhanced, while integrin and its ligands are downregulated in the cells with long telomeres. These results may provide valuable information related to telomere functions...
Phosphoproteomics for the discovery of kinases as cancer biomarkers and drug targetsLi Rong Yu
Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD, USA
Proteomics Clin Appl 1:1042-57. 2007..Strategies for global, targeted, and quantitative phosphoproteomics are discussed, and some recent interesting applications are also evaluated...- Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectraLi Rong Yu
Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, P O Box B, Frederick, Maryland 21702, USA
J Proteome Res 6:4150-62. 2007..The study also indicates that evaluation of confidence levels for phosphopeptide identification via the reversed sequence database searching strategy might underestimate the false positive rate... - On-column sample enrichment for capillary electrophoresis sheathless electrospray ionization mass spectrometry: evaluation for peptide analysis and protein identificationGeorge M Janini
Laboratory of Proteomics and Analytical Technologies and Image Analysis Laboratory, SAIC Frederick, Inc, National Cancer Institute at Frederick, P O Box B, Frederick, Maryland 21702, USA
Anal Chem 75:5984-93. 2003..Testing of this novel CE-MS system showed its ability to characterize proteomic samples such as protein digests, in-gel-digested proteins, and hydrophobic peptides as well as to quantitate ICAT-labeled peptides... - Combined chemical and enzymatic stable isotope labeling for quantitative profiling of detergent-insoluble membrane proteins isolated using Triton X-100 and Brij-96Josip Blonder
Laboratory of Proteomics and Analytical Technologies, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland 21702 1201, USA
J Proteome Res 5:349-60. 2006..This result confirms previous, targeted studies suggesting that DRMMs isolated using Triton X-100 and Brij-96 differ in their protein content... - Identification of a novel Wnt5a-CK1ɛ-Dvl2-Plk1-mediated primary cilia disassembly pathwayKyung Ho Lee
Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA
EMBO J 31:3104-17. 2012..This study may provide new insights into the mechanism underlying ciliary disassembly processes and various cilia-related disorders... - A detergent- and cyanogen bromide-free method for integral membrane proteomics: application to Halobacterium purple membranes and the human epidermal membrane proteomeJosip Blonder
SAIC-Frederick Inc, Laboratory of Proteomics and Analytical Technologies Mass Spectrometry Center, National Cancer Institute at Frederick, Frederick, MD 21702-1201, USA
Proteomics 4:31-45. 2004.... - Analysis of murine natural killer cell microsomal proteins using two-dimensional liquid chromatography coupled to tandem electrospray ionization mass spectrometryJosip Blonder
Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick, Inc, Frederick, Maryland 21702-1201, USA
J Proteome Res 3:862-70. 2004..These peptides resulted in identification of 2563 proteins of which 876 (34%) are classified as membrane proteins... - Increased oxidative-modifications of cytosolic proteins in 3,4-methylenedioxymethamphetamine (MDMA, ecstasy)-exposed rat liverVijay V Upreti
Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA
Proteomics 11:202-11. 2011..Our results show for the first time that MDMA induces oxidative-modification of many cytosolic proteins accompanied with increased oxidative stress and apoptosis, contributing to hepatic damage... - Quantitative profiling of the detergent-resistant membrane proteome of iota-b toxin induced vero cellsJosip Blonder
Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick, Inc, National Cancer Institute at Frederick, P.O. Box B, Frederick, Maryland 21702-1201, USA
J Proteome Res 4:523-31. 2005..The quantitative profiling of Ib-induced DRMP from Vero cells revealed several proteins with altered expression levels suggesting their possible role in Ib binding/uptake... - A combined proteome and microarray investigation of inorganic phosphate-induced pre-osteoblast cellsKelly A Conrads
Laboratory of Cancer Prevention, Center for Cancer Research, SAIC-Frederick, Inc, National Cancer Institute, Frederick, MD 21702, USA
Mol Cell Proteomics 4:1284-96. 2005..The ability to accurately measure changes in both protein abundance and mRNA levels on a system-wide scale represents a novel means to extract data from previously one-dimensional datasets... - A proteomic characterization of the plasma membrane of human epidermis by high-throughput mass spectrometryJosip Blonder
Laboratory of Proteomics and Analytical Technologies, Mass Spectrometry Center, SAIC-Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland, USA
J Invest Dermatol 123:691-9. 2004..Furthermore, 121 proteins from cholesterol-rich plasma membrane domains (caveolar and lipid rafts) were identified... - Diagnostic proteomics: serum proteomic patterns for the detection of early stage cancersLi-Rong Yu
Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick Inc, National Cancer Institute at Frederick, P.O. Box B, Frederick, MD 21702, USA
Dis Markers 19:209-18. 2003.... - Evaluation of the acid-cleavable isotope-coded affinity tag reagents: application to camptothecin-treated cortical neuronsLi-Rong Yu
Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick, Inc, National Cancer Institute at Frederick, P.O. Box B, Frederick, Maryland 21702-1201, USA
J Proteome Res 3:469-77. 2004..The new ICAT reagents were then applied to the analysis of a cortical neuron proteome sample to identify proteins regulated by the antitumor drug, camptothecin... - Global analysis of the cortical neuron proteomeLi-Rong Yu
Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick, Inc, National Cancer Institute at Frederick, P.O. Box B, Frederick, MD 21702-1201, USA
Mol Cell Proteomics 3:896-907. 2004..The results presented here provide the broadest proteome coverage for a mammalian cell to date and show that MS-based proteomics has the potential to provide high coverage of the proteins expressed within a cell... - Pooled ORF expression technology (POET): using proteomics to screen pools of open reading frames for protein expressionWilliam K Gillette
Protein Expression Laboratory, Research Technology Program, SAIC-Frederick, Inc./NCI, National Institutes of Health, Frederick, Maryland 21702, USA
Mol Cell Proteomics 4:1647-52. 2005..Using POET, pools of ORFs can be constructed, and the pools of the resulting proteins can be analyzed and manipulated to rapidly acquire information about the attributes of hundreds of proteins simultaneously... - Proteomic analysis by two-dimensional polyacrylamide gel electrophoresisMing Zhou
SAIC-Frederick, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA
Adv Protein Chem 65:57-84. 2003 - Quantitative proteomic analysis of inorganic phosphate-induced murine MC3T3-E1 osteoblast cellsKelly A Conrads
Laboratory of Cancer Prevention, Center for Cancer Research, Fredrick, MD, USA
Electrophoresis 25:1342-52. 2004..41-fold increase as determined by Western blotting. These results demonstrate that quantitative proteomics is capable of providing a quantitative view of thousands of proteins in mammalian cells within a defined set of experiments... - Proteome analysis of DNA damage-induced neuronal death using high throughput mass spectrometryMark D Johnson
Department of Neurological Surgery, University of Washington School of Medicine, Seattle, Washington 98195-6470, USA
J Biol Chem 279:26685-97. 2004..These data demonstrate the power of high throughput mass spectrometry for quantitative analysis of the neuronal proteome... - Proteome analysis of camptothecin-treated cortical neurons using isotope-coded affinity tagsLi-Rong Yu
Pacific Northwest National Laboratory, Richland, WA, USA
Electrophoresis 23:1591-8. 2002..While proteins from most functional classes of proteins were identified, a particularly large percentage of the enzymes involved in glycolysis and the tricarboxylic acid cycle were observed... - Detection of in situ labeled cell surface proteins by mass spectrometry: application to the membrane subproteome of human mammary epithelial cellsWan-Nan U Chen
Cell Biology, Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA
Proteomics 3:1647-51. 2003..The method reported can be adapted to include stable isotope labeling of proteins for quantitation of changes occurring on the cell surface in response to specific perturbations... - Phosphoprotein isotope-coded solid-phase tag approach for enrichment and quantitative analysis of phosphopeptides from complex mixturesWei-Jun Qian
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P.O. Box 999, MSIN: K8-98, Richland, Washington 99352, USA
Anal Chem 75:5441-50. 2003..Its utility for proteomic applications was demonstrated by the labeling of soluble phosphoproteins from a human breast cancer cell line... - Multidimensional proteome analysis of human mammary epithelial cellsJon M Jacobs
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99352, USA
J Proteome Res 3:68-75. 2004.... - Profiling the activity of G proteins in patient-derived tissues by rapid affinity-capture of signal transduction proteins (GRASP)David M Berman
Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proteomics 4:812-8. 2004..GRASP represents an advance in the field of proteomics as it detects protein interactions present in cells as they exist in their native tissue microenvironment... - The proteomics of neurodegenerationMark D Johnson
Department of Neurological Surgery, University of Washington School of Medicine, Seattle, Washington 98195-6470, USA
Am J Pharmacogenomics 5:259-70. 2005.... - Self-regulated Plk1 recruitment to kinetochores by the Plk1-PBIP1 interaction is critical for proper chromosome segregationYoung H Kang
Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
Mol Cell 24:409-22. 2006..Thus, Plk1 self-regulates the Plk1-PBIP1 interaction to timely localize to the kinetochores and promote proper chromosome segregation... - Regulation of androgen receptor activity by tyrosine phosphorylationZhiyong Guo
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
Cancer Cell 10:309-19. 2006.... - Direct phosphorylation and activation of a Nim1-related kinase Gin4 by Elm1 in budding yeastSatoshi Asano
Laboratory of Metabolism, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 281:27090-8. 2006..Thus, Elm1 regulates the septin assembly-dependent cellular events by directly phosphorylating and activating the Gin4-dependent pathway(s)... - Parallel analysis of transcript and translation profiles: identification of metastasis-related signal pathways differentially regulated by drug and genetic modificationsHaiyan Yang
Laboratory of Population Genetics, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
J Proteome Res 5:1555-67. 2006.... - Quantitative proteomic analysis of sokotrasterol sulfate-stimulated primary human endothelial cellsAly Karsan
Department of Medical Biophysics, British Columbia Cancer Agency, Vancouver, British Columbia V5Z 1L3, Canada
Mol Cell Proteomics 4:191-204. 2005..These findings are consistent with a role for sokotrasterol sulfate in endothelial sprouting...