D Xia

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Inhibitor-complexed structures of the cytochrome bc1 from the photosynthetic bacterium Rhodobacter sphaeroides
    Lothar Esser
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 283:2846-57. 2008
  2. ncbi request reprint Structural Basis of Resistance to Anti-Cytochrome bc1 Complex Inhibitors: Implication for Drug Improvement
    Lothar Esser
    Laboratory of Cell Biology, NCI, NIH, 37 Convent Dr, Building 37, Room 2122C, Bethesda MD 20892
    Curr Pharm Des 20:704-24. 2014
  3. ncbi request reprint A novel electron transfer mechanism suggested by crystallographic studies of mitochondrial cytochrome bc1 complex
    D Xia
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas, Southwestern Medical Center, Dallas 75235, USA
    Biochem Cell Biol 76:673-9. 1998
  4. doi request reprint The road to the crystal structure of the cytochrome bc1 complex from the anoxigenic, photosynthetic bacterium Rhodobacter sphaeroides
    Di Xia
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Bioenerg Biomembr 40:485-92. 2008
  5. ncbi request reprint Structural basis for the mechanism of electron bifurcation at the quinol oxidation site of the cytochrome bc1 complex
    Di Xia
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, NIH, 37 Convent Dr, Building 37, Room 2122C, Bethesda, MD 20892, USA
    Photosynth Res 92:17-34. 2007
  6. ncbi request reprint Crystallographic investigation of peptide binding sites in the N-domain of the ClpA chaperone
    Di Xia
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Struct Biol 146:166-79. 2004
  7. ncbi request reprint Crystal structure of the cytochrome bc1 complex from bovine heart mitochondria
    D Xia
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75235, USA
    Science 277:60-6. 1997
  8. ncbi request reprint Crystal structure of the heterodimeric complex of the adaptor, ClpS, with the N-domain of the AAA+ chaperone, ClpA
    Fusheng Guo
    Laboratory of Cell Biology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:46753-62. 2002
  9. ncbi request reprint The crystal structure of mitochondrial cytochrome bc1 in complex with famoxadone: the role of aromatic-aromatic interaction in inhibition
    Xiugong Gao
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Biochemistry 41:11692-702. 2002
  10. ncbi request reprint Crystal structure of ClpA, an Hsp100 chaperone and regulator of ClpAP protease
    Fusheng Guo
    Laboratory of Cell Biology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:46743-52. 2002

Collaborators

Detail Information

Publications32

  1. ncbi request reprint Inhibitor-complexed structures of the cytochrome bc1 from the photosynthetic bacterium Rhodobacter sphaeroides
    Lothar Esser
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 283:2846-57. 2008
    ..Functional implications for the bc(1) complex are derived from analyses of 10 independent molecules in various crystal forms and from comparisons with mitochondrial complexes...
  2. ncbi request reprint Structural Basis of Resistance to Anti-Cytochrome bc1 Complex Inhibitors: Implication for Drug Improvement
    Lothar Esser
    Laboratory of Cell Biology, NCI, NIH, 37 Convent Dr, Building 37, Room 2122C, Bethesda MD 20892
    Curr Pharm Des 20:704-24. 2014
    ..Structural data analysis provides a clear understanding of where and why existing inhibitors fail and points towards promising alternatives. ..
  3. ncbi request reprint A novel electron transfer mechanism suggested by crystallographic studies of mitochondrial cytochrome bc1 complex
    D Xia
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas, Southwestern Medical Center, Dallas 75235, USA
    Biochem Cell Biol 76:673-9. 1998
    ..A novel electron transfer mechanism for the bc1 complex consistent with crystallographic observations is discussed...
  4. doi request reprint The road to the crystal structure of the cytochrome bc1 complex from the anoxigenic, photosynthetic bacterium Rhodobacter sphaeroides
    Di Xia
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Bioenerg Biomembr 40:485-92. 2008
    ..3 A resolution. The improved crystal quality can be understood in terms of participation of strontium ions in molecular packing arrangement in crystal...
  5. ncbi request reprint Structural basis for the mechanism of electron bifurcation at the quinol oxidation site of the cytochrome bc1 complex
    Di Xia
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, NIH, 37 Convent Dr, Building 37, Room 2122C, Bethesda, MD 20892, USA
    Photosynth Res 92:17-34. 2007
    ....
  6. ncbi request reprint Crystallographic investigation of peptide binding sites in the N-domain of the ClpA chaperone
    Di Xia
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Struct Biol 146:166-79. 2004
    ..A functional model is proposed in which the N-domains in ClpA function as tentacles to weakly hold on to proteins thereby enhancing local substrate concentration...
  7. ncbi request reprint Crystal structure of the cytochrome bc1 complex from bovine heart mitochondria
    D Xia
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75235, USA
    Science 277:60-6. 1997
    ..The proteins core 1 and core 2 are structurally similar to each other and consist of two domains of roughly equal size and identical folding topology...
  8. ncbi request reprint Crystal structure of the heterodimeric complex of the adaptor, ClpS, with the N-domain of the AAA+ chaperone, ClpA
    Fusheng Guo
    Laboratory of Cell Biology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:46753-62. 2002
    ....
  9. ncbi request reprint The crystal structure of mitochondrial cytochrome bc1 in complex with famoxadone: the role of aromatic-aromatic interaction in inhibition
    Xiugong Gao
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Biochemistry 41:11692-702. 2002
    ..These results support an inhibitory mechanism that is consistent with the requirement for ISP movement in the electron transfer of this complex...
  10. ncbi request reprint Crystal structure of ClpA, an Hsp100 chaperone and regulator of ClpAP protease
    Fusheng Guo
    Laboratory of Cell Biology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:46743-52. 2002
    ....
  11. ncbi request reprint Structural basis for the quinone reduction in the bc1 complex: a comparative analysis of crystal structures of mitochondrial cytochrome bc1 with bound substrate and inhibitors at the Qi site
    Xiugong Gao
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 42:9067-80. 2003
    ..A two-water-mediated ubiquinone protonation mechanism is proposed involving three Q(i) site residues His(201), Lys(227), and Asp(228)...
  12. ncbi request reprint The conserved tyrosine residues 401 and 1044 in ATP sites of human P-glycoprotein are critical for ATP binding and hydrolysis: evidence for a conserved subdomain, the A-loop in the ATP-binding cassette
    In Wha Kim
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 45:7605-16. 2006
    ..We named this subdomain the "A-loop" (aromatic residue interacting with the adenine ring of ATP)...
  13. pmc Surface-modulated motion switch: capture and release of iron-sulfur protein in the cytochrome bc1 complex
    Lothar Esser
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:13045-50. 2006
    ..A mechanism for the high fidelity of the bifurcated electron transfer is proposed...
  14. ncbi request reprint Transcript mapping and transregulatory behavior of varicella-zoster virus gene 21, a latency-associated gene
    D Xia
    Laboratory of Clinical Investigation, National Institutes of Health, Bethesda, Maryland, 20892, USA
    Virology 258:304-13. 1999
    ..In transient expression assays, the ORF 21 showed no significant transregulatory activity on promoters of diverse kinetic classes. The ORF 21 promoter, however, was transactivated strongly by VZV infection or by ORF 62...
  15. pmc A novel ATP-dependent conformation in p97 N-D1 fragment revealed by crystal structures of disease-related mutants
    Wai Kwan Tang
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    EMBO J 29:2217-29. 2010
    ..We propose that IBMPFD mutations alter the timing of the transition between nucleotide states by destabilizing the ADP-bound form and consequently interfere with the interactions between the N-domains and their substrates...
  16. pmc Structure of CFA/I fimbriae from enterotoxigenic Escherichia coli
    Yong Fu Li
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4256, USA
    Proc Natl Acad Sci U S A 106:10793-8. 2009
    ....
  17. pmc Mutations define cross-talk between the N-terminal nucleotide-binding domain and transmembrane helix-2 of the yeast multidrug transporter Pdr5: possible conservation of a signaling interface for coupling ATP hydrolysis to drug transport
    Zuben E Sauna
    Laboratory of Cell Biology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892 4256, USA
    J Biol Chem 283:35010-22. 2008
    ....
  18. ncbi request reprint Crystallographic studies of quinol oxidation site inhibitors: a modified classification of inhibitors for the cytochrome bc(1) complex
    Lothar Esser
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4255, USA
    J Mol Biol 341:281-302. 2004
    ..Class P contains two subgroups, Pm and Pf, that are distinct by their ability to induce mobile or fixed conformation of iron-sulfur protein...
  19. ncbi request reprint A receptor-binding site as revealed by the crystal structure of CfaE, the colonization factor antigen I fimbrial adhesin of enterotoxigenic Escherichia coli
    Yong Fu Li
    Laboratory of Cell Biology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892 4256, USA
    J Biol Chem 282:23970-80. 2007
    ..The location of this well ordered donor strand suggests the positioning and orientation of the subjacent major fimbrial subunit CfaB in the native assembly of CFA/I fimbriae...
  20. ncbi request reprint Protein binding and disruption by Clp/Hsp100 chaperones
    Michael R Maurizi
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Structure 12:175-83. 2004
    ....
  21. ncbi request reprint The A-loop, a novel conserved aromatic acid subdomain upstream of the Walker A motif in ABC transporters, is critical for ATP binding
    Suresh V Ambudkar
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, DHHS, Bethesda, MD 20892 4256, USA
    FEBS Lett 580:1049-55. 2006
    ....
  22. pmc Crystallization and preliminary X-ray diffraction analysis of CfaE, the adhesive subunit of the CFA/I fimbriae from human enterotoxigenic Escherichia coli
    Yong Fu Li
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892 4256, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:121-4. 2006
    ..Sequence assignments were aided by anomalous signals from the selenium of an SeMet-derivatized crystal and from S atoms of a native crystal...
  23. pmc Crystallization and preliminary X-ray diffraction analyses of several forms of the CfaB major subunit of enterotoxigenic Escherichia coli CFA/I fimbriae
    Yong Fu Li
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892 4256, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:242-7. 2009
    ..3 A resolution and had the symmetry of space group P2(1)2(1)2. CfaBBB crystallized in the monoclinic space group C2 and diffracted X-rays to 2.3 A resolution. These structures were determined using the molecular-replacement method...
  24. ncbi request reprint Towards understanding the mechanism of action of the multidrug resistance-linked half-ABC transporter ABCG2: a molecular modeling study
    Yong Fu Li
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    J Mol Graph Model 25:837-51. 2007
    ..The apparent flexibility of the ICD1 may play a role in transmitting conformational changes from the NBD to the TMD or from the TMD to the NBD...
  25. pmc Purification, crystallization and preliminary X-ray diffraction analysis of disease-related mutants of p97
    Wai Kwan Tang
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:1166-70. 2009
    ..89, b = 102.6, c = 107.2 angstrom, alpha = 97.5, beta = 90.6, gamma = 91.5 degrees and a = 92.76, b = 103.7, c = 107.7 angstrom , alpha = 97.7, beta = 91.9, gamma = 89.7 degrees, respectively...
  26. pmc Mutational analysis of threonine 402 adjacent to the GXXXG dimerization motif in transmembrane segment 1 of ABCG2
    Orsolya Polgar
    Medical Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 10, Room 13N240, Bethesda, Maryland 20892, USA
    Biochemistry 49:2235-45. 2010
    ..Homology modeling of ABCG2 places the TXXXGXXXG motif at the dimer interface. These studies are consistent with a role for the extended TXXXGXXXG motif in ABCG2 folding, processing, and/or dimerization...
  27. doi request reprint Arginine 383 is a crucial residue in ABCG2 biogenesis
    Orsolya Polgar
    Medical Oncology Branch, National Cancer Institute, NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1788:1434-43. 2009
    ..In conclusion, arginine 383 is a crucial residue for ABCG2 biogenesis, where even the most conservative mutations have a large impact...
  28. ncbi request reprint The iron-sulfur cluster of the Rieske iron-sulfur protein functions as a proton-exiting gate in the cytochrome bc(1) complex
    Buddha Gurung
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biol Chem 280:24895-902. 2005
    ..It was speculated that in the normal catalytic cycle of the bc(1) complex, the [2Fe-2S] cluster may function as a proton-exiting gate...
  29. doi request reprint Domain conformational switch of the iron-sulfur protein in cytochrome bc1 complex is induced by the electron transfer from cytochrome bL to bH
    Chang An Yu
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
    Biochim Biophys Acta 1777:1038-43. 2008
    ....
  30. ncbi request reprint Evidence for electron equilibrium between the two hemes bL in the dimeric cytochrome bc1 complex
    Xing Gong
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biol Chem 280:9251-7. 2005
    ..This supports the idea that the interruption of electron transfer between the two bL hemes enhances electron leakage to oxygen and thus decreases the ubiquinol-cytochrome c reductase activity...
  31. ncbi request reprint Inter- and intra-molecular electron transfer in the cytochrome bc(1) complex
    Chang An Yu
    Department of Biochemistry and Molecular Biology, NRC 255, OAES, Oklahoma State University, Stillwater, OK74078, USA
    Biochim Biophys Acta 1555:65-70. 2002
    ....
  32. ncbi request reprint Generation, characterization and crystallization of a highly active and stable cytochrome bc1 complex mutant from Rhodobacter sphaeroides
    Maria Elberry
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, 74078, USA
    Biochim Biophys Acta 1757:835-40. 2006
    ..3 degrees C increase in the thermo-denaturation temperature. Crystals formed from this mutant complex, in the presence of stigmatellin, diffract X-rays up to 2.9 Angstroms resolution...