Graeme Wistow

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Platelet-derived growth factor D, tissue-specific expression in the eye, and a key role in control of lens epithelial cell proliferation
    Sugata Ray
    NEI, National Institutes of Health, Bethesda, Maryland 20892 0703, USA
    J Biol Chem 280:8494-502. 2005
  2. pmc The human crystallin gene families
    Graeme Wistow
    Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 0608, USA
    Hum Genomics 6:26. 2012
  3. pmc Expressed sequence tag analysis of adult human optic nerve for NEIBank: identification of cell type and tissue markers
    Steven L Bernstein
    Department of Ophthalmology, University of Maryland School of Medicine, Baltimore, Maryland, USA
    BMC Neurosci 10:121. 2009
  4. ncbi request reprint The human gene for gammaS-crystallin: alternative transcripts and expressed sequences from the first intron
    G Wistow
    Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
    Mol Vis 6:79-84. 2000
  5. pmc NEIBank: genomics and bioinformatics resources for vision research
    Graeme Wistow
    Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 0703, USA
    Mol Vis 14:1327-37. 2008
  6. ncbi request reprint The NEIBank project for ocular genomics: data-mining gene expression in human and rodent eye tissues
    Graeme Wistow
    Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Building 7, Room 201, Bethesda, MD 20892 0703, USA
    Prog Retin Eye Res 25:43-77. 2006
  7. ncbi request reprint gammaN-crystallin and the evolution of the betagamma-crystallin superfamily in vertebrates
    Graeme Wistow
    National Eye Institute, National Institutes of Health, Bethesda, MD 20892 0703, USA
    FEBS J 272:2276-91. 2005
  8. ncbi request reprint Grouping and identification of sequence tags (GRIST): bioinformatics tools for the NEIBank database
    Graeme Wistow
    Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
    Mol Vis 8:164-70. 2002
  9. ncbi request reprint Expressed sequence tag analysis of adult human lens for the NEIBank Project: over 2000 non-redundant transcripts, novel genes and splice variants
    Graeme Wistow
    Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
    Mol Vis 8:171-84. 2002
  10. ncbi request reprint Expressed sequence tag analysis of human retina for the NEIBank Project: retbindin, an abundant, novel retinal cDNA and alternative splicing of other retina-preferred gene transcripts
    Graeme Wistow
    Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
    Mol Vis 8:196-204. 2002

Collaborators

Detail Information

Publications33

  1. ncbi request reprint Platelet-derived growth factor D, tissue-specific expression in the eye, and a key role in control of lens epithelial cell proliferation
    Sugata Ray
    NEI, National Institutes of Health, Bethesda, Maryland 20892 0703, USA
    J Biol Chem 280:8494-502. 2005
    ..Intervention in the PDGF-D pathway in the eye, perhaps by antibody or blocking peptide, could be useful in the treatment of certain cataracts, including post-operative secondary cataract...
  2. pmc The human crystallin gene families
    Graeme Wistow
    Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 0608, USA
    Hum Genomics 6:26. 2012
    ..Crystallin organization is essential for lens transparency and mutations; even minor changes to surface residues can cause cataract and loss of vision...
  3. pmc Expressed sequence tag analysis of adult human optic nerve for NEIBank: identification of cell type and tissue markers
    Steven L Bernstein
    Department of Ophthalmology, University of Maryland School of Medicine, Baltimore, Maryland, USA
    BMC Neurosci 10:121. 2009
    ..Quantitative real time polymerase chain reaction (qRT-PCR) and Western analysis were used to compare levels of expression for key calcium channel protein genes and protein product in primate and rodent ON...
  4. ncbi request reprint The human gene for gammaS-crystallin: alternative transcripts and expressed sequences from the first intron
    G Wistow
    Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
    Mol Vis 6:79-84. 2000
    ..gammaS-crystallins are major components of adult vertebrate lenses. Here we examine the population of gammaS transcripts in adult human lens and the structure of the human CRYGS genes...
  5. pmc NEIBank: genomics and bioinformatics resources for vision research
    Graeme Wistow
    Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 0703, USA
    Mol Vis 14:1327-37. 2008
    ..NEIBank provides a comprehensive overview of current knowledge of the transcriptional repertoires of eye tissues and their relation to pathology...
  6. ncbi request reprint The NEIBank project for ocular genomics: data-mining gene expression in human and rodent eye tissues
    Graeme Wistow
    Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Building 7, Room 201, Bethesda, MD 20892 0703, USA
    Prog Retin Eye Res 25:43-77. 2006
    ..These point to specific adaptations and mechanisms in the human eye and emphasize that care needs to be taken in the application of appropriate animal model systems...
  7. ncbi request reprint gammaN-crystallin and the evolution of the betagamma-crystallin superfamily in vertebrates
    Graeme Wistow
    National Eye Institute, National Institutes of Health, Bethesda, MD 20892 0703, USA
    FEBS J 272:2276-91. 2005
    ..Although well conserved throughout vertebrate evolution, gammaN in primates has apparently undergone major changes and possible loss of functional expression...
  8. ncbi request reprint Grouping and identification of sequence tags (GRIST): bioinformatics tools for the NEIBank database
    Graeme Wistow
    Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
    Mol Vis 8:164-70. 2002
    ..The NEIBank web site contains a growing set of pages cataloging the known transcriptional repertoire of eye tissues, derived from new NEIBank cDNA libraries and from eye-related data deposited in the dbEST section of GenBank...
  9. ncbi request reprint Expressed sequence tag analysis of adult human lens for the NEIBank Project: over 2000 non-redundant transcripts, novel genes and splice variants
    Graeme Wistow
    Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
    Mol Vis 8:171-84. 2002
    ..To explore the expression profile of the human lens and to provide a resource for microarray studies, expressed sequence tag (EST) analysis has been performed on cDNA libraries from adult lenses...
  10. ncbi request reprint Expressed sequence tag analysis of human retina for the NEIBank Project: retbindin, an abundant, novel retinal cDNA and alternative splicing of other retina-preferred gene transcripts
    Graeme Wistow
    Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
    Mol Vis 8:196-204. 2002
    ....
  11. ncbi request reprint Expressed sequence tag analysis of human RPE/choroid for the NEIBank Project: over 6000 non-redundant transcripts, novel genes and splice variants
    Graeme Wistow
    Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
    Mol Vis 8:205-20. 2002
    ..Here we describe expressed sequence tag (EST) analysis of human RPE/choroid as part of a project for ocular bioinformatics...
  12. ncbi request reprint Expressed sequence tag analysis of adult human iris for the NEIBank Project: steroid-response factors and similarities with retinal pigment epithelium
    Graeme Wistow
    Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
    Mol Vis 8:185-95. 2002
    ..Expressed sequence tag (EST) analysis of human iris has been performed to explore the repertoire of genes expressed in this tissue...
  13. ncbi request reprint A project for ocular bioinformatics: NEIBank
    Graeme Wistow
    Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
    Mol Vis 8:161-3. 2002
  14. pmc A role for lengsin, a recruited enzyme, in terminal differentiation in the vertebrate lens
    Keith Wyatt
    National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 283:6607-15. 2008
    ..This suggests that lengsin may act as a component of the cytoskeleton itself or as a chaperone for the reorganization of intermediate filament proteins during terminal differentiation in the lens...
  15. pmc Lengsin is a survivor of an ancient family of class I glutamine synthetases re-engineered by evolution for a role in the vertebrate lens
    Keith Wyatt
    Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Structure 14:1823-34. 2006
    ..Genes related to Lengsin are also present in the sea urchin, suggesting that this branch of the GS I family, supplanted by GS II enzymes in vertebrates, has an ancient role in metazoans...
  16. ncbi request reprint Gene expression profile of the rat eye iridocorneal angle: NEIBank expressed sequence tag analysis
    Farid Ahmed
    Section of Molecular Mechanisms of Glaucoma, Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, MD 20814 9692, USA
    Invest Ophthalmol Vis Sci 45:3081-90. 2004
    ..To characterize gene expression pattern in the combined tissues of the rat iridocorneal angle by expressed sequence tag (EST) analysis, as part of the NEIBank project...
  17. pmc Interaction of complement factor h and fibulin3 in age-related macular degeneration
    M Keith Wyatt
    Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 8:e68088. 2013
    ..The CFH 402H/Fib3 interaction could contribute to the development of pathological aggregates in soft drusen in some patients and as such might provide a target for therapeutic intervention in some forms of AMD. ..
  18. ncbi request reprint Gene expression profile of the human trabecular meshwork: NEIBank sequence tag analysis
    Stanislav I Tomarev
    Laboratory of Molecular and Developmental Biology, National Institutes of Health, Bethesda, Maryland 20892 2730, USA
    Invest Ophthalmol Vis Sci 44:2588-96. 2003
    ..To characterize the gene expression pattern in the human trabecular meshwork (TM) and identify candidate genes for glaucoma by expressed sequence tag (EST) analysis as part of the NEIBank project...
  19. ncbi request reprint Specific interaction between lens MIP/Aquaporin-0 and two members of the gamma-crystallin family
    Jianguo Fan
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 0704, USA
    Mol Vis 11:76-87. 2005
    ..Our goal was to examine the interaction or lack of interaction between MIP and all members of the gamma-crystallin family and to provide evidence for a physiological role these interactions may play in gamma-crystallin or MIP function...
  20. ncbi request reprint Gene expression profile of the mouse organ of Corti at the onset of hearing
    Celine Pompeia
    Section on Structural Cell Biology, NIDCD, National Institutes of Health, Building 50 Room 4249, 50 South Drive, Bethesda, MD 20892, USA
    Genomics 83:1000-11. 2004
    ..This resource provides both a view of the profile of gene expression in the OC at the onset of hearing and a tool to identify novel genes of importance in hearing...
  21. pmc Endogenous retroviral insertion in Cryge in the mouse No3 cataract mutant
    Nabanita Nag
    Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Building 7, Room 201 Bethesda, MD 20892, USA
    Genomics 89:512-20. 2007
    ..However, the severity of cataract is also strain dependent suggesting that genetic background modifiers also play a role in the development of opacity...
  22. pmc A role for γS-crystallin in the organization of actin and fiber cell maturation in the mouse lens
    Jianguo Fan
    Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 0608, USA
    FEBS J 279:2892-904. 2012
    ..This suggests that γS may have a functional role related to actin, perhaps in 'shepherding' filaments to maintain the optical properties of the lens cytoplasm and normal fiber cell maturation...
  23. pmc Solution structure of (gamma)S-crystallin by molecular fragment replacement NMR
    Zhengrong Wu
    Building 5, Room 126, NIH, Bethesda, MD 20892 0520, USA
    Protein Sci 14:3101-14. 2005
    ..The method used for solving the gammaS structure relies on the recently introduced molecular fragment replacement method, which capitalizes on the large database of protein structures previously solved by X-ray crystallography and NMR...
  24. doi request reprint Solution properties of γ-crystallins: Compact structure and low frictional ratio are conserved properties of diverse γ-crystallins
    Yingwei Chen
    Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Bethesda, Maryland, 20892 0608
    Protein Sci 23:76-87. 2014
    ..The solution behavior of γ-crystallins, with highly compact structures suited for the densely packed environment of the lens, seems to be highly conserved and appears largely independent of amino acid composition. ..
  25. pmc Lengsin expression and function during zebrafish lens formation
    Rachel L Harding
    University of Notre Dame, Department of Biological Sciences and Center for Zebrafish Research, Galvin Life Science Center, Notre Dame, IN 46556 0369, USA
    Exp Eye Res 86:807-18. 2008
    ..These results demonstrate Lengsin is required for proper fiber cell differentiation by playing roles in either cell elongation or the establishment of cell interactions...
  26. pmc Development of a microarray chip for gene expression in rabbit ocular research
    Michael P Popp
    University of Florida s Interdisciplinary Center for Biotechnology Research, University of Florida, Gainsville, FL 32610 0294, USA
    Mol Vis 13:164-73. 2007
    ..To develop a microarray for the rabbit that can be used for ocular gene expression research...
  27. pmc Defining the human macula transcriptome and candidate retinal disease genes using EyeSAGE
    Catherine Bowes Rickman
    Department of Ophthalmology, Duke University Medical Center, Durham, NC 27710, USA
    Invest Ophthalmol Vis Sci 47:2305-16. 2006
    ....
  28. ncbi request reprint Expressed sequence tag analysis of zebrafish eye tissues for NEIBank
    Thomas S Vihtelic
    Center for Zebrafish Research, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
    Mol Vis 11:1083-100. 2005
    ..To characterize gene expression patterns in various tissues of the zebrafish (Danio rerio) eye and identify zebrafish orthologs of human genes by expressed sequence tag (EST) analysis for NEIBank...
  29. ncbi request reprint Gene expression profile studies of human keratoconus cornea for NEIBank: a novel cornea-expressed gene and the absence of transcripts for aquaporin 5
    Yaron S Rabinowitz
    Cornea Genetic Eye Institute, Cedars Sinai Medical Center, Los Angeles, California, USA
    Invest Ophthalmol Vis Sci 46:1239-46. 2005
    ..To increase the database of genes expressed in human cornea and to gain insights into the molecular basis of keratoconus (KC)...
  30. ncbi request reprint EST analysis of mouse retina and RPE/choroid cDNA libraries
    Hisashi Ida
    Department of Biological Chemistry and Ophthalmology, University of California, Davis, CA 95616 8794, USA
    Mol Vis 10:439-44. 2004
    ..To complement these libraries and to provide the first mouse RPE/choroid library, we used freshly dissected tissue from adult C57BL/6J mice to construct new retina and RPE/choroid libraries...
  31. ncbi request reprint Retina-specific expression of 5A11/Basigin-2, a member of the immunoglobulin gene superfamily
    Judith D Ochrietor
    Whitney Laboratory of the University of Florida, St Augustine, Florida 32080, USA
    Invest Ophthalmol Vis Sci 44:4086-96. 2003
    ..Previous reports indicate the presence of multiple 5A11/Basigin polypeptides within the retina. Therefore, in an effort to determine the function of 5A11/Basigin, the molecular diversity of its expression was evaluated...
  32. ncbi request reprint Stability, homodimerization, and calcium-binding properties of a single, variant betagamma-crystallin domain of the protein absent in melanoma 1 (AIM1)
    Bheemreddy Rajini
    Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India
    Biochemistry 42:4552-9. 2003
    ..These results show that, despite the sequence variation, AIM1-g1 folds such as a betagamma domain, binds calcium and undergoes dimerization...
  33. pmc Exploring the limits of sequence and structure in a variant betagamma-crystallin domain of the protein absent in melanoma-1 (AIM1)
    Penmatsa Aravind
    Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India
    J Mol Biol 381:509-18. 2008
    ..This structure illustrates the resilience of the betagamma fold to considerable sequence changes and its remarkable ability to adapt for novel functions...