Research Topics
Genomes and Genes
| Graeme WistowSummaryAffiliation: National Institutes of Health Country: USA Publications
| Collaborators
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Detail Information
Publications
Platelet-derived growth factor D, tissue-specific expression in the eye, and a key role in control of lens epithelial cell proliferationSugata Ray
NEI, National Institutes of Health, Bethesda, Maryland 20892 0703, USA
J Biol Chem 280:8494-502. 2005..Intervention in the PDGF-D pathway in the eye, perhaps by antibody or blocking peptide, could be useful in the treatment of certain cataracts, including post-operative secondary cataract...
The human crystallin gene familiesGraeme Wistow
Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 0608, USA
Hum Genomics 6:26. 2012..Crystallin organization is essential for lens transparency and mutations; even minor changes to surface residues can cause cataract and loss of vision...- Expressed sequence tag analysis of adult human optic nerve for NEIBank: identification of cell type and tissue markersSteven L Bernstein
Department of Ophthalmology, University of Maryland School of Medicine, Baltimore, Maryland, USA
BMC Neurosci 10:121. 2009..Quantitative real time polymerase chain reaction (qRT-PCR) and Western analysis were used to compare levels of expression for key calcium channel protein genes and protein product in primate and rodent ON... - NEIBank: genomics and bioinformatics resources for vision researchGraeme Wistow
Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 0703, USA
Mol Vis 14:1327-37. 2008..NEIBank provides a comprehensive overview of current knowledge of the transcriptional repertoires of eye tissues and their relation to pathology... - The NEIBank project for ocular genomics: data-mining gene expression in human and rodent eye tissuesGraeme Wistow
Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Building 7, Room 201, Bethesda, MD 20892 0703, USA
Prog Retin Eye Res 25:43-77. 2006..These point to specific adaptations and mechanisms in the human eye and emphasize that care needs to be taken in the application of appropriate animal model systems... - gammaN-crystallin and the evolution of the betagamma-crystallin superfamily in vertebratesGraeme Wistow
National Eye Institute, National Institutes of Health, Bethesda, MD 20892 0703, USA
FEBS J 272:2276-91. 2005..Although well conserved throughout vertebrate evolution, gammaN in primates has apparently undergone major changes and possible loss of functional expression... - The human gene for gammaS-crystallin: alternative transcripts and expressed sequences from the first intronG Wistow
Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
Mol Vis 6:79-84. 2000..gammaS-crystallins are major components of adult vertebrate lenses. Here we examine the population of gammaS transcripts in adult human lens and the structure of the human CRYGS genes... - Grouping and identification of sequence tags (GRIST): bioinformatics tools for the NEIBank databaseGraeme Wistow
Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
Mol Vis 8:164-70. 2002..The NEIBank web site contains a growing set of pages cataloging the known transcriptional repertoire of eye tissues, derived from new NEIBank cDNA libraries and from eye-related data deposited in the dbEST section of GenBank... - Expressed sequence tag analysis of adult human lens for the NEIBank Project: over 2000 non-redundant transcripts, novel genes and splice variantsGraeme Wistow
Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
Mol Vis 8:171-84. 2002..To explore the expression profile of the human lens and to provide a resource for microarray studies, expressed sequence tag (EST) analysis has been performed on cDNA libraries from adult lenses... - Expressed sequence tag analysis of human RPE/choroid for the NEIBank Project: over 6000 non-redundant transcripts, novel genes and splice variantsGraeme Wistow
Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
Mol Vis 8:205-20. 2002..Here we describe expressed sequence tag (EST) analysis of human RPE/choroid as part of a project for ocular bioinformatics... - Expressed sequence tag analysis of human retina for the NEIBank Project: retbindin, an abundant, novel retinal cDNA and alternative splicing of other retina-preferred gene transcriptsGraeme Wistow
Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
Mol Vis 8:196-204. 2002.... - Expressed sequence tag analysis of adult human iris for the NEIBank Project: steroid-response factors and similarities with retinal pigment epitheliumGraeme Wistow
Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
Mol Vis 8:185-95. 2002..Expressed sequence tag (EST) analysis of human iris has been performed to explore the repertoire of genes expressed in this tissue... - A project for ocular bioinformatics: NEIBankGraeme Wistow
Section on Molecular Structure and Function, National Eye Institute, National Institutes of Health, Bethesda, MD 20892-2740, USA
Mol Vis 8:161-3. 2002 - A role for lengsin, a recruited enzyme, in terminal differentiation in the vertebrate lensKeith Wyatt
National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Biol Chem 283:6607-15. 2008..This suggests that lengsin may act as a component of the cytoskeleton itself or as a chaperone for the reorganization of intermediate filament proteins during terminal differentiation in the lens... - Lengsin is a survivor of an ancient family of class I glutamine synthetases re-engineered by evolution for a role in the vertebrate lensKeith Wyatt
Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Structure 14:1823-34. 2006..Genes related to Lengsin are also present in the sea urchin, suggesting that this branch of the GS I family, supplanted by GS II enzymes in vertebrates, has an ancient role in metazoans... - Gene expression profile of the rat eye iridocorneal angle: NEIBank expressed sequence tag analysisFarid Ahmed
Section of Molecular Mechanisms of Glaucoma, Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, MD 20814 9692, USA
Invest Ophthalmol Vis Sci 45:3081-90. 2004..To characterize gene expression pattern in the combined tissues of the rat iridocorneal angle by expressed sequence tag (EST) analysis, as part of the NEIBank project... - Specific interaction between lens MIP/Aquaporin-0 and two members of the gamma-crystallin familyJianguo Fan
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 0704, USA
Mol Vis 11:76-87. 2005..Our goal was to examine the interaction or lack of interaction between MIP and all members of the gamma-crystallin family and to provide evidence for a physiological role these interactions may play in gamma-crystallin or MIP function... - Gene expression profile of the human trabecular meshwork: NEIBank sequence tag analysisStanislav I Tomarev
Laboratory of Molecular and Developmental Biology, National Institutes of Health, Bethesda, Maryland 20892 2730, USA
Invest Ophthalmol Vis Sci 44:2588-96. 2003..To characterize the gene expression pattern in the human trabecular meshwork (TM) and identify candidate genes for glaucoma by expressed sequence tag (EST) analysis as part of the NEIBank project... - Gene expression profile of the mouse organ of Corti at the onset of hearingCeline Pompeia
Section on Structural Cell Biology, NIDCD, National Institutes of Health, Building 50 Room 4249, 50 South Drive, Bethesda, MD 20892, USA
Genomics 83:1000-11. 2004..This resource provides both a view of the profile of gene expression in the OC at the onset of hearing and a tool to identify novel genes of importance in hearing... - Endogenous retroviral insertion in Cryge in the mouse No3 cataract mutantNabanita Nag
Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Building 7, Room 201 Bethesda, MD 20892, USA
Genomics 89:512-20. 2007..However, the severity of cataract is also strain dependent suggesting that genetic background modifiers also play a role in the development of opacity... - A role for γS-crystallin in the organization of actin and fiber cell maturation in the mouse lensJianguo Fan
Section on Molecular Structure and Functional Genomics, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 0608, USA
FEBS J 279:2892-904. 2012..This suggests that γS may have a functional role related to actin, perhaps in 'shepherding' filaments to maintain the optical properties of the lens cytoplasm and normal fiber cell maturation... - Solution structure of (gamma)S-crystallin by molecular fragment replacement NMRZhengrong Wu
Building 5, Room 126, NIH, Bethesda, MD 20892 0520, USA
Protein Sci 14:3101-14. 2005..The method used for solving the gammaS structure relies on the recently introduced molecular fragment replacement method, which capitalizes on the large database of protein structures previously solved by X-ray crystallography and NMR... - Lengsin expression and function during zebrafish lens formationRachel L Harding
University of Notre Dame, Department of Biological Sciences and Center for Zebrafish Research, Galvin Life Science Center, Notre Dame, IN 46556 0369, USA
Exp Eye Res 86:807-18. 2008..These results demonstrate Lengsin is required for proper fiber cell differentiation by playing roles in either cell elongation or the establishment of cell interactions... - Stability, homodimerization, and calcium-binding properties of a single, variant betagamma-crystallin domain of the protein absent in melanoma 1 (AIM1)Bheemreddy Rajini
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India
Biochemistry 42:4552-9. 2003..These results show that, despite the sequence variation, AIM1-g1 folds such as a betagamma domain, binds calcium and undergoes dimerization... - Development of a microarray chip for gene expression in rabbit ocular researchMichael P Popp
University of Florida s Interdisciplinary Center for Biotechnology Research, University of Florida, Gainsville, FL 32610 0294, USA
Mol Vis 13:164-73. 2007..To develop a microarray for the rabbit that can be used for ocular gene expression research... - Defining the human macula transcriptome and candidate retinal disease genes using EyeSAGECatherine Bowes Rickman
Department of Ophthalmology, Duke University Medical Center, Durham, NC 27710, USA
Invest Ophthalmol Vis Sci 47:2305-16. 2006.... - Expressed sequence tag analysis of zebrafish eye tissues for NEIBankThomas S Vihtelic
Center for Zebrafish Research, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
Mol Vis 11:1083-100. 2005..To characterize gene expression patterns in various tissues of the zebrafish (Danio rerio) eye and identify zebrafish orthologs of human genes by expressed sequence tag (EST) analysis for NEIBank... - EST analysis of mouse retina and RPE/choroid cDNA librariesHisashi Ida
Department of Biological Chemistry and Ophthalmology, University of California, Davis, CA 95616-8794, USA
Mol Vis 10:439-44. 2004..CONCLUSIONS: These new libraries should provide a valuable resource for gene discovery and cDNAs for expression analysis and functional studies... - Retina-specific expression of 5A11/Basigin-2, a member of the immunoglobulin gene superfamilyJudith D Ochrietor
Whitney Laboratory of the University of Florida, St Augustine, Florida 32080, USA
Invest Ophthalmol Vis Sci 44:4086-96. 2003..Previous reports indicate the presence of multiple 5A11/Basigin polypeptides within the retina. Therefore, in an effort to determine the function of 5A11/Basigin, the molecular diversity of its expression was evaluated... - Gene expression profile studies of human keratoconus cornea for NEIBank: a novel cornea-expressed gene and the absence of transcripts for aquaporin 5Yaron S Rabinowitz
Cornea Genetic Eye Institute, Cedars Sinai Medical Center, Los Angeles, California, USA
Invest Ophthalmol Vis Sci 46:1239-46. 2005..To increase the database of genes expressed in human cornea and to gain insights into the molecular basis of keratoconus (KC)... - Exploring the limits of sequence and structure in a variant betagamma-crystallin domain of the protein absent in melanoma-1 (AIM1)Penmatsa Aravind
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India
J Mol Biol 381:509-18. 2008..This structure illustrates the resilience of the betagamma fold to considerable sequence changes and its remarkable ability to adapt for novel functions...