Allan M Weissman

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Working on a chain: E3s ganging up for ubiquitylation
    Meredith B Metzger
    Meredith B Metzger and Allan M Weissman Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick MD 21702, USA
    Nat Cell Biol 12:1124-6. 2010
  2. doi request reprint How much REST is enough?
    Allan M Weissman
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, Frederick, MD 20712, USA
    Cancer Cell 13:381-3. 2008
  3. ncbi request reprint Themes and variations on ubiquitylation
    A M Weissman
    Laboratory of Immune Cell Biology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892 1152, USA
    Nat Rev Mol Cell Biol 2:169-78. 2001
  4. pmc The predator becomes the prey: regulating the ubiquitin system by ubiquitylation and degradation
    Allan M Weissman
    Laboratory of Protein Dynamics and Signalling, National Cancer Institute, Frederick, Maryland 21702, USA
    Nat Rev Mol Cell Biol 12:605-20. 2011
  5. doi request reprint A structurally unique E2-binding domain activates ubiquitination by the ERAD E2, Ubc7p, through multiple mechanisms
    Meredith B Metzger
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702 1201, USA
    Mol Cell 50:516-27. 2013
  6. ncbi request reprint WW domain HECT E3s target Cbl RING finger E3s for proteasomal degradation
    Alessandra Magnifico
    Regulation of Protein Function Laboratory, Center for Cancer Research, NCI Frederick, Frederick, MD 21702, USA
    J Biol Chem 278:43169-77. 2003
  7. pmc Allosteric regulation of E2:E3 interactions promote a processive ubiquitination machine
    Ranabir Das
    Structural Biophysics Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA
    EMBO J 32:2504-16. 2013
  8. ncbi request reprint Inhibitors of ubiquitin-activating enzyme (E1), a new class of potential cancer therapeutics
    Yili Yang
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute at Frederick, NIH, Frederick, Maryland 21702, USA
    Cancer Res 67:9472-81. 2007
  9. pmc Stress-induced phosphorylation and proteasomal degradation of mitofusin 2 facilitates mitochondrial fragmentation and apoptosis
    Guillaume P Leboucher
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA
    Mol Cell 47:547-57. 2012
  10. pmc Structural basis for ubiquitin recognition and autoubiquitination by Rabex-5
    Sangho Lee
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, US National Institutes of Health NIH, Bethesda, Maryland 20892, USA
    Nat Struct Mol Biol 13:264-71. 2006

Collaborators

Detail Information

Publications62

  1. ncbi request reprint Working on a chain: E3s ganging up for ubiquitylation
    Meredith B Metzger
    Meredith B Metzger and Allan M Weissman Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick MD 21702, USA
    Nat Cell Biol 12:1124-6. 2010
    ..These interactions and the related concept of E4 activity are discussed...
  2. doi request reprint How much REST is enough?
    Allan M Weissman
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, Frederick, MD 20712, USA
    Cancer Cell 13:381-3. 2008
    ..These findings and their significance are discussed herein...
  3. ncbi request reprint Themes and variations on ubiquitylation
    A M Weissman
    Laboratory of Immune Cell Biology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892 1152, USA
    Nat Rev Mol Cell Biol 2:169-78. 2001
    ..A few years ago, we could only have dreamt of the complex arsenal of enzymes dedicated to ubiquitylation. Why has nature come up with so many ways of doing what seems to be such a simple job?..
  4. pmc The predator becomes the prey: regulating the ubiquitin system by ubiquitylation and degradation
    Allan M Weissman
    Laboratory of Protein Dynamics and Signalling, National Cancer Institute, Frederick, Maryland 21702, USA
    Nat Rev Mol Cell Biol 12:605-20. 2011
    ..This observation has broad implications for pathophysiology...
  5. doi request reprint A structurally unique E2-binding domain activates ubiquitination by the ERAD E2, Ubc7p, through multiple mechanisms
    Meredith B Metzger
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702 1201, USA
    Mol Cell 50:516-27. 2013
    ..This demonstrates that an essential component of E3 complexes can simultaneously bind to E2 and enhance its loading with ubiquitin. These findings provide mechanistic insights into how ubiquitination can be stimulated...
  6. ncbi request reprint WW domain HECT E3s target Cbl RING finger E3s for proteasomal degradation
    Alessandra Magnifico
    Regulation of Protein Function Laboratory, Center for Cancer Research, NCI Frederick, Frederick, MD 21702, USA
    J Biol Chem 278:43169-77. 2003
    ..These findings establish that RING finger E3s can be substrates, not only for autoubiquitylation but also for ubiquitylation by HECT E3s and suggest an additional level of regulation for Cbl substrates including protein-tyrosine kinases...
  7. pmc Allosteric regulation of E2:E3 interactions promote a processive ubiquitination machine
    Ranabir Das
    Structural Biophysics Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA
    EMBO J 32:2504-16. 2013
    ..Thus, gp78 is a ubiquitination machine where multiple E2-binding sites coordinately facilitate processive ubiquitination. ..
  8. ncbi request reprint Inhibitors of ubiquitin-activating enzyme (E1), a new class of potential cancer therapeutics
    Yili Yang
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute at Frederick, NIH, Frederick, Maryland 21702, USA
    Cancer Res 67:9472-81. 2007
    ..These inhibitors can also be valuable tools for studying ubiquitylation...
  9. pmc Stress-induced phosphorylation and proteasomal degradation of mitofusin 2 facilitates mitochondrial fragmentation and apoptosis
    Guillaume P Leboucher
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA
    Mol Cell 47:547-57. 2012
    ..These findings demonstrate how proximal signaling events can influence both mitochondrial dynamics and apoptosis through phosphorylation-stimulated degradation of the mitochondrial fusion machinery...
  10. pmc Structural basis for ubiquitin recognition and autoubiquitination by Rabex-5
    Sangho Lee
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, US National Institutes of Health NIH, Bethesda, Maryland 20892, USA
    Nat Struct Mol Biol 13:264-71. 2006
    ..The A20 zinc-finger diaromatic patch mediates ubiquitin-ligase activity by directly recruiting a ubiquitin-loaded ubiquitin-conjugating enzyme...
  11. pmc A Ubc7p-binding domain in Cue1p activates ER-associated protein degradation
    Zlatka Kostova
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA
    J Cell Sci 122:1374-81. 2009
    ..Thus, discrete E2 binding sites independent of ubiquitin ligase domains have the potential to activate ubiquitylation...
  12. ncbi request reprint Small molecule inhibitors of HDM2 ubiquitin ligase activity stabilize and activate p53 in cells
    Yili Yang
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute at Frederick, NIH, 1050 Boyles Street, Frederick, MD 21702, USA
    Cancer Cell 7:547-59. 2005
    ..In cells, the compounds allow the stabilization of p53 and HDM2 and activation of p53-dependent transcription and apoptosis, although other p53-independent toxicity was also observed...
  13. ncbi request reprint The ubiquitin ligase gp78 promotes sarcoma metastasis by targeting KAI1 for degradation
    Yien Che Tsai
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA
    Nat Med 13:1504-9. 2007
    ....
  14. pmc Sequential requirements for the GTPase domain of the mitofusin Fzo1 and the ubiquitin ligase SCFMdm30 in mitochondrial outer membrane fusion
    Mickael M Cohen
    Center for Cancer Research, NCI Frederick, MD 21702, USA
    J Cell Sci 124:1403-10. 2011
    ..These findings suggest a sequence of events in early mitochondrial fusion where Fzo1 GTPase-domain-dependent tethering leads to recruitment of SCF(Mdm30) and ubiquitin-mediated degradation of Fzo1, which facilitates mitochondrial fusion...
  15. pmc Allosteric activation of E2-RING finger-mediated ubiquitylation by a structurally defined specific E2-binding region of gp78
    Ranabir Das
    Structural Biophysics Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702 1201, USA
    Mol Cell 34:674-85. 2009
    ..These findings uncover a mechanism whereby allosteric effects on an E2 enhance E2-RING finger interactions and, consequently, ubiquitylation...
  16. ncbi request reprint CAIR-1/BAG-3 abrogates heat shock protein-70 chaperone complex-mediated protein degradation: accumulation of poly-ubiquitinated Hsp90 client proteins
    Howard Doong
    Molecular Signaling Section, Laboratory of Pathology and Regulation of Protein Function Laboratory, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 278:28490-500. 2003
    ..Furthermore, poly-ubiquitination is not sufficient for efficient proteasomal targeting of Hsp client proteins...
  17. doi request reprint Studies of the ubiquitin proteasome system
    Kevin L Lorick
    National Cancer Institute, Frederick, Maryland, USA
    Curr Protoc Cell Biol . 2006
    ..Because another protein modifier, NEDD8, itself regulates aspects of the ubiquitin system, basic protocols on neddylation are also included in this unit...
  18. pmc The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site
    Bo Chen
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, Building 560, Room 22 103, National Cancer Institute, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 103:341-6. 2006
    ..These results also provide proof of principle that interrupting a specific E2-E3 interaction can selectively inhibit ERAD...
  19. ncbi request reprint The Rab5 guanine nucleotide exchange factor Rabex-5 binds ubiquitin (Ub) and functions as a Ub ligase through an atypical Ub-interacting motif and a zinc finger domain
    Rafael Mattera
    Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 281:6874-83. 2006
    ..Moreover, the demonstration that Rabex-5 is a ubiquitin ligase that binds ubiquitin and undergoes ubiquitination indicates that its role in endosome fusion may be subject to additional regulation by ubiquitin-dependent modifications...
  20. ncbi request reprint Mdm2 is a RING finger-dependent ubiquitin protein ligase for itself and p53
    S Fang
    Laboratory of Immune Cell Biology, Division of Basic Sciences, NCI, National Institutes of Health, Bethesda, Maryland 20892 1152, USA
    J Biol Chem 275:8945-51. 2000
    ..However, this RING was ineffective in ubiquitination and proteasomal targeting of p53, suggesting that there may be specificity at the level of the RING in the recognition of heterologous substrates...
  21. pmc Targeting of gp78 for ubiquitin-mediated proteasomal degradation by Hrd1: cross-talk between E3s in the endoplasmic reticulum
    Ayelet Shmueli
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, NCI, Frederick 21702, USA
    Biochem Biophys Res Commun 390:758-62. 2009
    ....
  22. pmc Ubiquitin-proteasome-dependent degradation of a mitofusin, a critical regulator of mitochondrial fusion
    Mickael M J Cohen
    Laboratory of Protein Dynamics and Signaling, National Cancer Institute, Frederick, MD 21702, USA
    Mol Biol Cell 19:2457-64. 2008
    ..This study provides a framework for developing an understanding of the function of Mdm30p-mediated Fzo1p degradation in the multistep process of mitochondrial fusion...
  23. pmc Serine residues in the cytosolic tail of the T-cell antigen receptor alpha-chain mediate ubiquitination and endoplasmic reticulum-associated degradation of the unassembled protein
    Shuhei Ishikura
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver NICHD, Bethesda, MD 20892, USA
    J Biol Chem 285:23916-24. 2010
    ..We also found that this ubiquitination was mediated by the ER-localized ubiquitin ligase, HRD1. These findings indicate that serine-dependent, HRD1-mediated ubiquitination targets TCRalpha to the ERAD pathway...
  24. ncbi request reprint Expression and evaluation of RING finger proteins
    Yili Yang
    Dynamics and Signaling, National Cancer Institute at Frederick, Frederick, MD 21702, USA
    Methods Enzymol 398:103-12. 2005
    ..Use of these methods may help identify new E3s, dissect factors involved in ubiquitylation of substrates, and screen for molecules that affect ubiquitylation...
  25. pmc Differential regulation of HMG-CoA reductase and Insig-1 by enzymes of the ubiquitin-proteasome system
    Yien Che Tsai
    Laboratory of Protein Dynamics and Signaling, National Cancer Institute, Frederick, MD 20712, USA
    Mol Biol Cell 23:4484-94. 2012
    ..Our results suggest a need for additional studies before definitive mechanistic conclusions are drawn that might set the stage for development of drugs to manipulate gp78 function in metabolic disorders...
  26. pmc Promiscuous interactions of gp78 E3 ligase CUE domain with polyubiquitin chains
    Shan Liu
    Structural Biophysics Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702 1201, USA
    Structure 20:2138-50. 2012
    ..This leads to a model in which the CUE domain functions to both facilitate substrate binding and enable switching between adjacent ubiquitin molecules of a growing chain to enable processivity in ubiquitination...
  27. doi request reprint Identification of inhibitors for MDM2 ubiquitin ligase activity from natural product extracts by a novel high-throughput electrochemiluminescent screen
    CHRISTY A SASIELA
    Molecular Targets Development Program, National Cancer Institute, Frederick, Maryland 21702, USA
    J Biomol Screen 13:229-37. 2008
    ..Sempervirine preferentially induced apoptosis in transformed cells expressing wild-type p53, suggesting that it could be a potential lead for anticancer therapeutics...
  28. ncbi request reprint BTBD1 and BTBD2 colocalize to cytoplasmic bodies with the RBCC/tripartite motif protein, TRIM5delta
    Lixin Xu
    Department of Biochemistry and Molecular Biology, F Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814 4799, USA
    Exp Cell Res 288:84-93. 2003
    ..Thus, four protein modules found on each of these putative ubiquitin ligases, a RING, a B-box and two PHR repeats, are present on BTBD1/2 and TRIM5delta that are colocalized to cytoplasmic bodies...
  29. pmc Dissecting the diverse functions of the metastasis suppressor CD82/KAI1
    Yien Che Tsai
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702, United States
    FEBS Lett 585:3166-73. 2011
    ..A common feature of these diverse effects is CD82 regulation of membrane organization as well as protein trafficking and interactions, which affects cellular signaling and intercellular communication...
  30. pmc Discovery of new pyridoacridine alkaloids from Lissoclinum cf. badium that inhibit the ubiquitin ligase activity of Hdm2 and stabilize p53
    Jason A Clement
    Molecular Targets Development Program, Center for Cancer Research, National Cancer Institute Frederick, National Institutes of Health, Frederick, MD 21702 1201, USA
    Bioorg Med Chem 16:10022-8. 2008
    ..badium. Lissoclinidine B inhibited ubiquitylation and degradation of p53, and selectively killed transformed cells harboring wild-type p53, suggesting this compound could be used to develop new treatments...
  31. pmc Targeting botulinum neurotoxin persistence by the ubiquitin-proteasome system
    Yien Che Tsai
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 107:16554-9. 2010
    ..We describe chimeric SNAP25-based ubiquitin ligases that target BoNT/A LC for degradation, reducing its duration in a cellular model for toxin persistence...
  32. pmc Ubiquitin ligases, critical mediators of endoplasmic reticulum-associated degradation
    Zlatka Kostova
    Laboratory of Protein Dynamics and Signaling, Building 560 Room 22 103, Center for Cancer Research, National Cancer Institute at Frederick, National Institutes of Health, MD 21702, United States
    Semin Cell Dev Biol 18:770-9. 2007
    ..In this chapter we review our knowledge of both Saccharomyces cerevisiae and mammalian ERAD ubiquitin ligases. We focus on recent insights into these E3s, their associated proteins and potential mechanisms of action...
  33. ncbi request reprint Expression, purification, and properties of the Ubc4/5 family of E2 enzymes
    Kevin L Lorick
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA
    Methods Enzymol 398:54-68. 2005
    ..Using the UbcH5 family as a prototype, this chapter describes methods for the expression, purification, and characterization of E2 enzymes in vitro and some of the basics for their use in experiments in cells...
  34. ncbi request reprint Identification of developmentally expressed proteins that functionally interact with Nedd4 ubiquitin ligase
    Rodolfo Murillas
    Experimental Immunology Branch, NCI, National Institutes of Health, Bethesda, Maryland 20892 1360, USA
    J Biol Chem 277:2897-907. 2002
    ..However, this protein clearly associates with Nedd4 through its PY domains and can alter the location of Nedd4 in cells, suggesting a role other than as a ubiquitylation substrate...
  35. ncbi request reprint RING finger ubiquitin protein ligases: implications for tumorigenesis, metastasis and for molecular targets in cancer
    Shengyun Fang
    Regulation of Protein Function Laboratory, Center for Cancer Research, National Cancer Institute at Frederick, Building 560, Room 22 95, 1050 Boyles Street, Frederick, MD 21702, USA
    Semin Cancer Biol 13:5-14. 2003
    ....
  36. ncbi request reprint Mind bomb is a ubiquitin ligase that is essential for efficient activation of Notch signaling by Delta
    Motoyuki Itoh
    Laboratory of Molecular Genetics, NICHD, NIH, Bethesda, MD 20892, USA
    Dev Cell 4:67-82. 2003
    ..This facilitates intramembranous cleavage of the remaining Notch receptor, release of the Notch intracellular fragment, and activation of target genes in neighboring cells...
  37. pmc Targeting tumor cells expressing p53 with a water-soluble inhibitor of Hdm2
    Jirouta Kitagaki
    Laboratory of Protein Dynamics and Signaling, National Cancer Institute at Frederick, 1050 Boyles Street, Frederick, MD 21702, USA
    Mol Cancer Ther 7:2445-54. 2008
    ..These results suggest that HLI373 could serve as a potential lead for developing cancer therapeutics based on inhibition of the ubiquitin ligase activity of Hdm2...
  38. ncbi request reprint Ubiquitin and the control of protein fate in the secretory and endocytic pathways
    J S Bonifacino
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 5430, USA
    Annu Rev Cell Dev Biol 14:19-57. 1998
    ..These recent findings imply that ubiquitin plays more diverse roles in the regulation of the fate of cellular proteins than originally anticipated...
  39. pmc Ubiquitylation in ERAD: reversing to go forward?
    Yien Che Tsai
    Laboratory of Protein Dynamics and Signaling, National Cancer Institute, Frederick, Maryland, United States of America
    PLoS Biol 9:e1001038. 2011
    ..The implications of this work for understanding ERAD and the potential of expressing deubiquitylating enzyme domains for studying ubiquitin-mediated processes are discussed...
  40. ncbi request reprint Subcellular localization and ubiquitin-conjugating enzyme (E2) interactions of mammalian HECT family ubiquitin protein ligases
    S Hatakeyama
    Laboratory of Immune Cell Biology, Division of Basic Sciences, NCI, National Institutes of Health, Bethesda, Maryland 20892 1152, USA
    J Biol Chem 272:15085-92. 1997
    ..Furthermore, the presence of two E2 binding sites within Nedd-4 suggests models for ubiquitination involving multiple E2 enzymes associated with E3s...
  41. pmc The tumor autocrine motility factor receptor, gp78, is a ubiquitin protein ligase implicated in degradation from the endoplasmic reticulum
    S Fang
    Regulation of Protein Function Laboratory, Center for Cancer Research, National Cancer Institute, Building 10, Room 1B34, 9000 Rockville Pike, Bethesda, MD 20892 1152, USA
    Proc Natl Acad Sci U S A 98:14422-7. 2001
    ..gp78 has thus been found to be an example of a mammalian cellular E3 intrinsic to the ER, suggesting a potential link between ubiquitylation, ERAD, and metastasis...
  42. ncbi request reprint Regulating the p53 system through ubiquitination
    Yili Yang
    Regulation of Protein Function Laboratory, Center for Cancer Research, National Cancer Institute Frederick, 1050 Boyles Street, 560 22 64, Frederick, MD 21702, USA
    Oncogene 23:2096-106. 2004
    ..It is conceivable that new chemotherapeutic agents based on these studies will be generated in the not-so-distant future...
  43. pmc RINGs of good and evil: RING finger ubiquitin ligases at the crossroads of tumour suppression and oncogenesis
    Stanley Lipkowitz
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892, USA
    Nat Rev Cancer 11:629-43. 2011
    ..As a result, many RING finger E3s are implicated in either the suppression or the progression of cancer. This Review summarizes current knowledge in this area...
  44. pmc RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination
    K L Lorick
    Laboratory of Immune Cell Biology, Division of Basic Sciences, National Cancer Institute, Building 10, Room 1B34, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 1152, USA
    Proc Natl Acad Sci U S A 96:11364-9. 1999
    ..These findings suggest that a large number of RING finger-containing proteins, with otherwise diverse structures and functions, may play previously unappreciated roles in modulating protein levels via ubiquitination...
  45. ncbi request reprint Endoplasmic reticulum (ER)-associated degradation of T cell receptor subunits. Involvement of ER-associated ubiquitin-conjugating enzymes (E2s)
    S Tiwari
    Laboratory of Immune Cell Biology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892 1152, USA
    J Biol Chem 276:16193-200. 2001
    ..These findings also implicate, for the first time, a specific E2 in degradation from the endoplasmic reticulum in mammalian cells...
  46. doi request reprint RING-type E3 ligases: Master manipulators of E2 ubiquitin-conjugating enzymes and ubiquitination
    Meredith B Metzger
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, 1050 Boyles Street, Frederick, MD 21702, USA
    Biochim Biophys Acta 1843:47-60. 2014
    ..This article is part of a Special Issue entitled: Ubiquitin-Proteasome System. Guest Editors: Thomas Sommer and Dieter H. Wolf. ..
  47. pmc Compact Parkin only: insights into the structure of an autoinhibited ubiquitin ligase
    R Andrew Byrd
    Structural Biophysics Laboratory, Center for Cancer Research National Cancer Institute, Frederick, MD, USA
    EMBO J 32:2087-9. 2013
    ..The important advances from these studies set the stage for the next steps in understanding the molecular basis for Parkinson's disease (PD)...
  48. pmc A ubiquitin-binding rhomboid protease aimed at ERADication
    Yien Che Tsai
    Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, 1050 Boyles Street, Frederick, MD 21701, USA
    Dev Cell 23:454-6. 2012
    ..2012) describe a role for a ubiquitin-binding rhomboid protease, RHBDL4, in degradation of select ERAD substrates. These findings and the significance of rhomboids and other intramembrane proteases are discussed...
  49. doi request reprint Solubilization of lymphocytes
    Allan M Weissman
    National Cancer Institute, Bethesda, Maryland, USA
    Curr Protoc Immunol . 2003
    ..In some situations, it may be desirable to purify membranes prior to their solubilization or to determine the physical relationship between proteins, which can be accomplished by a cross-linking...
  50. pmc Differences in the tumor microenvironment between African-American and European-American breast cancer patients
    Damali N Martin
    Laboratory of Human Carcinogenesis, Center of Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 4:e4531. 2009
    ..We tested the hypothesis that intrinsic differences in the tumor biology may contribute to this cancer health disparity...
  51. ncbi request reprint Identification of a family of closely related human ubiquitin conjugating enzymes
    J P Jensen
    Laboratory of Immune Cell Biology, National Cancer Institute, Bethesda, Maryland 20892 1152, USA
    J Biol Chem 270:30408-14. 1995
    ..These results establish the existence of a highly conserved, and widely expressed, family of human ubiquitin conjugating enzymes...
  52. ncbi request reprint Elf-1 regulates basal expression from the T cell antigen receptor zeta-chain gene promoter
    B L Rellahan
    Laboratory of Immunobiology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    J Immunol 160:2794-801. 1998
    ..Taken together with the existing literature, these data also suggest that the requirement for inducible factors in Elf-1-mediated trans-activation may decrease as the affinity and number of Elf-1 sites increase...
  53. ncbi request reprint Cbl-b-dependent coordinated degradation of the epidermal growth factor receptor signaling complex
    S A Ettenberg
    Genetics Department of the Medicine Branch and the Laboratory of Immune Cell Biology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20889, USA
    J Biol Chem 276:27677-84. 2001
    ..Furthermore, the data demonstrate that Cbl-b mediates degradation of multiple proteins in the EGFR-signaling complex...
  54. ncbi request reprint SINAT5 promotes ubiquitin-related degradation of NAC1 to attenuate auxin signals
    Qi Xie
    Laboratory of Molecular Cell Biology, Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604 Singapore
    Nature 419:167-70. 2002
    ..Low expression of NAC1 in roots can be increased by treatment with a proteasome inhibitor, which indicates that SINAT5 targets NAC1 for ubiquitin-mediated proteolysis to downregulate auxin signals in plant cells...
  55. ncbi request reprint Overexpression of the tumor autocrine motility factor receptor Gp78, a ubiquitin protein ligase, results in increased ubiquitinylation and decreased secretion of apolipoprotein B100 in HepG2 cells
    Jun shan Liang
    Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    J Biol Chem 278:23984-8. 2003
    ..Together, these results indicate that an ER-associated protein, gp78, is a bona fide E3 ligase in the apoB ER-associated degradation pathway...
  56. ncbi request reprint Inositol 1,4,5-trisphosphate receptor ubiquitination is mediated by mammalian Ubc7, a component of the endoplasmic reticulum-associated degradation pathway, and is inhibited by chelation of intracellular Zn2+
    Jack M Webster
    Department of Pharmacology, State University of New York Upstate Medical University, Syracuse, New York 13210 2339, USA
    J Biol Chem 278:38238-46. 2003
    ..Finally, muscarinic agonist-induced InsP3R ubiquitination was seen in rat brain slices, indicating that the results obtained from SH-SY5Y cells reflect a physiological process...
  57. pmc A conserved catalytic residue in the ubiquitin-conjugating enzyme family
    Pei Ying Wu
    Department of Biochemistry and Molecular Biology Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, MD 21205, USA
    EMBO J 22:5241-50. 2003
    ..We propose that the conserved asparagine side chain stabilizes the oxyanion intermediate formed during lysine attack. The E2 asparagine is the first non-covalent catalytic group to be proposed in any Ub conjugation factor...
  58. pmc EGF receptor-independent action of TGF-alpha protects Naked2 from AO7-mediated ubiquitylation and proteasomal degradation
    Wei Ding
    Departments of Cell and Developmental Biology and Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Proc Natl Acad Sci U S A 105:13433-8. 2008
    ..These results identify an EGFR-independent action of TGF-alpha, in which it protects Naked2 from proteasomal degradation, thus ensuring its delivery to the basolateral surface of polarized epithelial cells...
  59. pmc Nedd4 mediates agonist-dependent ubiquitination, lysosomal targeting, and degradation of the beta2-adrenergic receptor
    Sudha K Shenoy
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 283:22166-76. 2008
    ..Collectively, our findings indicate that the degradative fate of the beta(2)AR in the lysosomal compartments is dependent upon beta-arrestin2-mediated recruitment of Nedd4 to the activated receptor and Nedd4-catalyzed ubiquitination...
  60. pmc Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import
    Scott M Plafker
    Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    J Cell Biol 167:649-59. 2004
    ..Together, these findings reveal that Ub charging can function as a nuclear import trigger, and identify a novel link between E2 regulation and karyopherin-mediated transport...
  61. ncbi request reprint Multiple roles of Rbx1 in the VBC-Cul2 ubiquitin ligase complex
    Yuzuru Megumi
    Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, 1 4 3 Asahi machi, Abeno Ku, Osaka 545 8585, Japan
    Genes Cells 10:679-91. 2005
    ..Taken together, these results indicate that various mechanisms regulate both the activities and the stability of cullin-based ligases...
  62. ncbi request reprint E6AP mediates regulated proteasomal degradation of the nuclear receptor coactivator amplified in breast cancer 1 in immortalized cells
    Aparna Mani
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20007, USA
    Cancer Res 66:8680-6. 2006
    ..From our results, we propose a model whereby signals promoted by changes in the cellular milieu initiate E6AP-mediated proteasomal degradation of AIB1 and thus contribute to the control of steady-state levels of this protein...