Norman R Watts

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi Non-canonical binding of an antibody resembling a naïve B cell receptor immunoglobulin to hepatitis B virus capsids
    Norman R Watts
    Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Mol Biol 379:1119-29. 2008
  2. ncbi Molecular basis for the high degree of antigenic cross-reactivity between hepatitis B virus capsids (HBcAg) and dimeric capsid-related protein (HBeAg): insights into the enigmatic nature of the e-antigen
    Norman R Watts
    Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Mol Biol 398:530-41. 2010
  3. ncbi Structure, assembly, and antigenicity of hepatitis B virus capsid proteins
    Alasdair C Steven
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Adv Virus Res 64:125-64. 2005
  4. ncbi Role of the propeptide in controlling conformation and assembly state of hepatitis B virus e-antigen
    Norman R Watts
    Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Mol Biol 409:202-13. 2011
  5. ncbi The morphogenic linker peptide of HBV capsid protein forms a mobile array on the interior surface
    Norman R Watts
    Protein Expression Laboratory, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South Drive MSC 8025, National Institutes of Health, Bethesda, MD 20892-8025, USA
    EMBO J 21:876-84. 2002
  6. ncbi Cryo-EM study of Hepatitis B virus core antigen capsids decorated with antibodies from a human patient
    Eaazhisai Kandiah
    Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Struct Biol 177:145-51. 2012
  7. ncbi Generation and characterization of a chimeric rabbit/human Fab for co-crystallization of HIV-1 Rev
    Stephen J Stahl
    Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892 2775, USA
    J Mol Biol 397:697-708. 2010
  8. ncbi Specificity of an anti-capsid antibody associated with Hepatitis B Virus-related acute liver failure
    Weimin Wu
    Laboratory of Structural Biology, National Institute for Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, United States
    J Struct Biol 181:53-60. 2013
  9. ncbi The cryptophycin-tubulin ring structure indicates two points of curvature in the tubulin dimer
    Norman R Watts
    Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 41:12662-9. 2002
  10. ncbi Cryo-electron microscopy of trichocyte (hard alpha-keratin) intermediate filaments reveals a low-density core
    Norman R Watts
    Laboratory of Structural Biology Research, National Institutes of Health, Bethesda, Maryland 20892 8025, USA
    J Struct Biol 137:109-18. 2002

Collaborators

Detail Information

Publications12

  1. ncbi Non-canonical binding of an antibody resembling a naïve B cell receptor immunoglobulin to hepatitis B virus capsids
    Norman R Watts
    Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Mol Biol 379:1119-29. 2008
    ..Considerations of conserved sequence motifs in other such molecules suggest that other naïve B cells may interact with HBV capsids in much the same way...
  2. ncbi Molecular basis for the high degree of antigenic cross-reactivity between hepatitis B virus capsids (HBcAg) and dimeric capsid-related protein (HBeAg): insights into the enigmatic nature of the e-antigen
    Norman R Watts
    Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Mol Biol 398:530-41. 2010
    ..These results suggest new approaches for the isolation of the authentic e-antigen, its biological assay, and its stabilization as an immune complex for structural studies...
  3. ncbi Structure, assembly, and antigenicity of hepatitis B virus capsid proteins
    Alasdair C Steven
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Adv Virus Res 64:125-64. 2005
  4. ncbi Role of the propeptide in controlling conformation and assembly state of hepatitis B virus e-antigen
    Norman R Watts
    Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Mol Biol 409:202-13. 2011
    ..However, this intercalation distorts the dimer into an assembly-reluctant conformation...
  5. ncbi The morphogenic linker peptide of HBV capsid protein forms a mobile array on the interior surface
    Norman R Watts
    Protein Expression Laboratory, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South Drive MSC 8025, National Institutes of Health, Bethesda, MD 20892-8025, USA
    EMBO J 21:876-84. 2002
    ..We propose that linker peptides are attached to the capsid inner surface as hinged struts, forming a mobile array, an arrangement with implications for morphogenesis and the management of encapsidated nucleic acid...
  6. ncbi Cryo-EM study of Hepatitis B virus core antigen capsids decorated with antibodies from a human patient
    Eaazhisai Kandiah
    Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Struct Biol 177:145-51. 2012
    ..These results show that epitopes on the floor, far (~30 Å) from the immunodominant loop, are clinically relevant and that murine anti-cAg antibodies afford a good model for the human system...
  7. ncbi Generation and characterization of a chimeric rabbit/human Fab for co-crystallization of HIV-1 Rev
    Stephen J Stahl
    Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892 2775, USA
    J Mol Biol 397:697-708. 2010
    ..The corresponding single-chain antibody (scFv) was also prepared, offering the potential of intracellular antibody therapeutics against human immunodeficiency virus type 1...
  8. ncbi Specificity of an anti-capsid antibody associated with Hepatitis B Virus-related acute liver failure
    Weimin Wu
    Laboratory of Structural Biology, National Institute for Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, United States
    J Struct Biol 181:53-60. 2013
    ....
  9. ncbi The cryptophycin-tubulin ring structure indicates two points of curvature in the tubulin dimer
    Norman R Watts
    Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 41:12662-9. 2002
    ..We conclude that drug binding to one subunit (beta) results in two bends per dimer, affecting both subunits...
  10. ncbi Cryo-electron microscopy of trichocyte (hard alpha-keratin) intermediate filaments reveals a low-density core
    Norman R Watts
    Laboratory of Structural Biology Research, National Institutes of Health, Bethesda, Maryland 20892 8025, USA
    J Struct Biol 137:109-18. 2002
    ..3 nm. These filaments appear to represent IF coated with associated proteins-perhaps, "high-sulfur" proteins-readied for incorporation into the filament-matrix biocomposite of the mature hair...
  11. ncbi High-resolution mass spectrometry of viral assemblies: molecular composition and stability of dimorphic hepatitis B virus capsids
    Charlotte Uetrecht
    Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA, Utrecht, The Netherlands
    Proc Natl Acad Sci U S A 105:9216-20. 2008
    ..4 GPa. This experimental approach, anchored on very precise and accurate mass measurements, appears to hold considerable potential for elucidating the assembly of viruses and other macromolecular particles...
  12. ncbi Stability and shape of hepatitis B virus capsids in vacuo
    Charlotte Uetrecht
    Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands
    Angew Chem Int Ed Engl 47:6247-51. 2008