K Tilly

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Borrelia burgdorferi OspC protein required exclusively in a crucial early stage of mammalian infection
    Kit Tilly
    Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, MT 59840, USA
    Infect Immun 74:3554-64. 2006
  2. ncbi request reprint Infectious cycle analysis of a Borrelia burgdorferi mutant defective in transport of chitobiose, a tick cuticle component
    Kit Tilly
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
    Vector Borne Zoonotic Dis 4:159-68. 2004
  3. pmc Genetics and regulation of chitobiose utilization in Borrelia burgdorferi
    K Tilly
    Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana 59840, USA
    J Bacteriol 183:5544-53. 2001
  4. ncbi request reprint Isolation of Borrelia burgdorferi genes encoding homologues of DNA-binding protein HU and ribosomal protein S20
    K Tilly
    Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840, USA
    Microbiology 142:2471-9. 1996
  5. pmc Characterization of circular plasmid dimers in Borrelia burgdorferi
    K Tilly
    Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840, USA
    J Bacteriol 180:5676-81. 1998
  6. ncbi request reprint The Borrelia burgdorferi circular plasmid cp26: conservation of plasmid structure and targeted inactivation of the ospC gene
    K Tilly
    Laboratory of Microbial Structure and Function, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, Hamilton, MT 59840, USA
    Mol Microbiol 25:361-73. 1997
  7. pmc Efficient targeted mutagenesis in Borrelia burgdorferi
    J L Bono
    Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Disease, National Institutes of Health, Hamilton, Montana 59840, USA
    J Bacteriol 182:2445-52. 2000
  8. pmc Characterization of cp18, a naturally truncated member of the cp32 family of Borrelia burgdorferi plasmids
    B Stevenson
    Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840, USA
    J Bacteriol 179:4285-91. 1997
  9. ncbi request reprint Isolation of dnaJ, dnaK, and grpE homologues from Borrelia burgdorferi and complementation of Escherichia coli mutants
    K Tilly
    Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840
    Mol Microbiol 7:359-69. 1993
  10. ncbi request reprint Oligopeptide permease in Borrelia burgdorferi: putative peptide-binding components encoded by both chromosomal and plasmid loci
    J L Bono
    Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840, USA
    Microbiology 144:1033-44. 1998

Detail Information

Publications33

  1. pmc Borrelia burgdorferi OspC protein required exclusively in a crucial early stage of mammalian infection
    Kit Tilly
    Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, MT 59840, USA
    Infect Immun 74:3554-64. 2006
    ..We conclude that OspC is indispensable for establishing infection by B. burgdorferi in mammals but is not required at any other point of the mouse-tick infection cycle...
  2. ncbi request reprint Infectious cycle analysis of a Borrelia burgdorferi mutant defective in transport of chitobiose, a tick cuticle component
    Kit Tilly
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
    Vector Borne Zoonotic Dis 4:159-68. 2004
    ..These results demonstrate that B. burgdorferi growth in vivo is independent of chitobiose transport, even in an environmental niche in which the sugar is likely to be present...
  3. pmc Genetics and regulation of chitobiose utilization in Borrelia burgdorferi
    K Tilly
    Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana 59840, USA
    J Bacteriol 183:5544-53. 2001
    ..We propose renaming the three genes chbA, chbB, and chbC, since they probably encode a chitobiose transporter. We also found that the chbC gene was regulated in response to growth temperature and during growth in medium lacking GlcNAc...
  4. ncbi request reprint Isolation of Borrelia burgdorferi genes encoding homologues of DNA-binding protein HU and ribosomal protein S20
    K Tilly
    Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840, USA
    Microbiology 142:2471-9. 1996
    ..coli gene encoding ribosomal protein S20 and the orfH gene encodes a protein of unknown function. This operon is located upstream of the previously identified B. burgdorferi rho homologue...
  5. pmc Characterization of circular plasmid dimers in Borrelia burgdorferi
    K Tilly
    Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840, USA
    J Bacteriol 180:5676-81. 1998
    ..These serendipitous findings provide a mechanism for obtaining heterozygous complemented control strains when mutant phenotypes are characterized...
  6. ncbi request reprint The Borrelia burgdorferi circular plasmid cp26: conservation of plasmid structure and targeted inactivation of the ospC gene
    K Tilly
    Laboratory of Microbial Structure and Function, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, Hamilton, MT 59840, USA
    Mol Microbiol 25:361-73. 1997
    ..This first example of directed gene inactivation in B. burgdorferi shows that the OspC protein is not required for stable maintenance of cp26 or growth in culture...
  7. pmc Efficient targeted mutagenesis in Borrelia burgdorferi
    J L Bono
    Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Disease, National Institutes of Health, Hamilton, Montana 59840, USA
    J Bacteriol 182:2445-52. 2000
    ..The kanamycin resistance marker allows efficient direct selection of mutants in B. burgdorferi and hence is a significant improvement in the ability to construct isogenic mutant strains in this pathogen...
  8. pmc Characterization of cp18, a naturally truncated member of the cp32 family of Borrelia burgdorferi plasmids
    B Stevenson
    Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840, USA
    J Bacteriol 179:4285-91. 1997
    ..These findings suggest that a relatively small recombinant plasmid capable of being stably maintained in B. burgdorferi could be constructed from a cp32 plasmid...
  9. ncbi request reprint Isolation of dnaJ, dnaK, and grpE homologues from Borrelia burgdorferi and complementation of Escherichia coli mutants
    K Tilly
    Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840
    Mol Microbiol 7:359-69. 1993
    ..The grpE homologue complemented an E. coli grpE mutant and the dnaJ homologue complemented an E. coli dnaJ mutant, whereas the B. burgdorferi dnaK gene did not complement dnaK mutants...
  10. ncbi request reprint Oligopeptide permease in Borrelia burgdorferi: putative peptide-binding components encoded by both chromosomal and plasmid loci
    J L Bono
    Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840, USA
    Microbiology 144:1033-44. 1998
    ..Insertional inactivation of a plasmid-encoded oppA gene demonstrates that it is not essential for growth in culture...
  11. pmc A family of genes located on four separate 32-kilobase circular plasmids in Borrelia burgdorferi B31
    B Stevenson
    Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA
    J Bacteriol 178:3508-16. 1996
    ..3-kb circular plasmid of B. burgdorferi sensu lato Ip2l. All four 32-kb plasmids can be maintained within a single bacterium, which may provide a model for the study of plasmid replication and segregation in B. burgdorferi...
  12. pmc Plasmid location of Borrelia purine biosynthesis gene homologs
    N Margolis
    Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840
    J Bacteriol 176:6427-32. 1994
    ..The unique plasmid location of these and perhaps other housekeeping genes may be a consequence of the segmented genomes in borreliae and reflect the need to adapt to both the arthropod and mammalian environments...
  13. pmc Altered stationary-phase response in a Borrelia burgdorferi rpoS mutant
    A F Elias
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    J Bacteriol 182:2909-18. 2000
    ..This rpoS mutant will be useful for studying regulation of gene expression in response to changing environmental conditions...
  14. pmc Directed insertion of a selectable marker into a circular plasmid of Borrelia burgdorferi
    P Rosa
    Laboratory of Microbial Structure and Function, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840, USA
    J Bacteriol 178:5946-53. 1996
    ..burgdorferi whereby a large segment of heterologous DNA (gyrB) has been inserted via homologous recombination with flanking sequences, thus demonstrating the feasibility of specific gene inactivation by allelic exchange...
  15. ncbi request reprint Growth of infectious and non-infectious B. burgdorferi at different salt concentrations
    A Elias
    Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana, USA
    Wien Klin Wochenschr 110:863-5. 1998
    ..Osmotic strength is an important physical parameter for growth of B. burgdorferi in vitro and could influence its ability to adapt and to establish an infection within ticks and mammals...
  16. ncbi request reprint Genetic studies in Borrelia burgdorferi
    P Rosa
    Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
    Wien Klin Wochenschr 110:859-62. 1998
    ..However, the available methods are incomplete and far from routine. We are currently improving existing methods as well as developing additional genetic tools with which to augment genetic studies in B. burgdorferi...
  17. pmc Transformation of the Lyme disease spirochete Borrelia burgdorferi with heterologous DNA
    B Stevenson
    Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    J Bacteriol 180:4850-5. 1998
    ..The ability to transform B. burgdorferi with heterologous DNA will now permit a wide range of experiments on the biology of these bacteria and their involvement in the many facets of Lyme disease...
  18. pmc Genetic basis for retention of a critical virulence plasmid of Borrelia burgdorferi
    Mollie W Jewett
    Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Mol Microbiol 66:975-90. 2007
    ..We conclude that the genetic linkage of critical physiological and virulence functions on cp26 is pertinent to its stable maintenance throughout the evolution of B. burgdorferi...
  19. pmc Outer-surface protein C of the Lyme disease spirochete: a protein induced in ticks for infection of mammals
    Dorothee Grimm
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South Fourth Street, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 101:3142-7. 2004
    ..The induction of a spirochetal virulence factor preceding the time and host in which it is required demonstrates a developmental sequence for transmission of this arthropod-borne pathogen...
  20. pmc Experimental assessment of the roles of linear plasmids lp25 and lp28-1 of Borrelia burgdorferi throughout the infectious cycle
    Dorothee Grimm
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 72:5938-46. 2004
    ..burgdorferi and will be valuable in assessing the roles of plasmids even in unsequenced B. burgdorferi strains...
  21. pmc A Borrelia burgdorferi homolog of the Escherichia coli rho gene
    K Tilly
    Laboratory of Microbial Structure and Function, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840
    Nucleic Acids Res 21:1040. 1993
  22. pmc Biology of infection with Borrelia burgdorferi
    Kit Tilly
    Laboratory of Zoonotic Pathogens, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT 59840, USA
    Infect Dis Clin North Am 22:217-34, v. 2008
    ..This article describes the basic biology of B burgdorferi and reviews some of the bacterial components required for infection of and survival in the mammalian and tick hosts...
  23. pmc OspC-independent infection and dissemination by host-adapted Borrelia burgdorferi
    Kit Tilly
    Laboratory of Zoonotic Pathogens, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana 59840, USA
    Infect Immun 77:2672-82. 2009
    ..The strict temporal control of B. burgdorferi outer surface protein gene expression may reflect immunological constraints rather than distinct functions...
  24. pmc The critical role of the linear plasmid lp36 in the infectious cycle of Borrelia burgdorferi
    Mollie W Jewett
    Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Mol Microbiol 64:1358-74. 2007
    ..This work establishes a vital role for lp36 in the infectious cycle of B. burgdorferi and identifies the bbk17 gene as a component of this plasmid that contributes to mammalian infectivity...
  25. pmc Rapid clearance of Lyme disease spirochetes lacking OspC from skin
    Kit Tilly
    Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 75:1517-9. 2007
    ..To delineate this requirement, we analyzed the clearance of ospC mutant spirochetes and found that they were eliminated within 48 h. We conclude that B. burgdorferi uses OspC to resist innate host defenses immediately after transmission...
  26. pmc Borrelia burgdorferi resistance to a major skin antimicrobial peptide is independent of outer surface lipoprotein content
    Amit Sarkar
    Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, NIAID, NIH, 903 S 4th Street, Hamilton, MT 59840, USA
    Antimicrob Agents Chemother 53:4490-4. 2009
    ..We conclude that the essential role of OspC is unrelated to resistance to this component of innate immunity...
  27. pmc Delineating the requirement for the Borrelia burgdorferi virulence factor OspC in the mammalian host
    Philip E Stewart
    Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, NIAID, NIH, 903 South 4th St, Hamilton, MT 59840, USA
    Infect Immun 74:3547-53. 2006
    ..These results reiterate the importance of OspC in mammalian infection and eliminate simple models of function for this enigmatic protein...
  28. ncbi request reprint The burgeoning molecular genetics of the Lyme disease spirochaete
    Patricia A Rosa
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 S 4th Street, Hamilton, Montana 59840, USA
    Nat Rev Microbiol 3:129-43. 2005
    ..Increased genetic analysis of B. burgdorferi should advance our understanding of the infectious cycle and the pathogenesis of Lyme disease...
  29. ncbi request reprint The plasmids of Borrelia burgdorferi: essential genetic elements of a pathogen
    Philip E Stewart
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th St, Hamilton, MT 59840, USA
    Plasmid 53:1-13. 2005
    ..B. burgdorferi now represents a prime system with which to address basic questions of plasmid biology and plasmid contributions to bacterial virulence and disease pathogenesis...
  30. pmc Plasmid stability during in vitro propagation of Borrelia burgdorferi assessed at a clonal level
    Dorothee Grimm
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 71:3138-45. 2003
    ..We therefore conclude that isogenicity of clones must be confirmed irrespective of their in vitro passage history...
  31. pmc Clonal polymorphism of Borrelia burgdorferi strain B31 MI: implications for mutagenesis in an infectious strain background
    Abdallah F Elias
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 70:2139-50. 2002
    ..Due to the instability of the genome with in vitro propagation, careful monitoring of plasmid content of derived mutants and complementation of inactivated genes will be crucial components of genetic studies with this pathogen...
  32. pmc Use of the Cre-lox recombination system to investigate the lp54 gene requirement in the infectious cycle of Borrelia burgdorferi
    Aaron Bestor
    Laboratory of Zoonotic Pathogens, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana 59840, USA
    Infect Immun 78:2397-407. 2010
    ..burgdorferi and surprisingly revealed that a large number of the highly conserved proteins encoded on lp54 are not required to complete the infectious cycle...
  33. ncbi request reprint Defining plasmids required by Borrelia burgdorferi for colonization of tick vector Ixodes scapularis (Acari: Ixodidae)
    Dorothee Grimm
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    J Med Entomol 42:676-84. 2005
    ..burgdorferi within the tick vector, and it begins to establish the genomic components required for persistence of this pathogen throughout its natural infectious cycle...