Douglas D Thomas

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Molecular mechanisms for discrete nitric oxide levels in cancer
    Lisa A Ridnour
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Building 10, Bethesda, MD 20892, USA
    Nitric Oxide 19:73-6. 2008
  2. ncbi request reprint Asbestos redirects nitric oxide signaling through rapid catalytic conversion to nitrite
    Douglas D Thomas
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Cancer Res 66:11600-4. 2006
  3. ncbi request reprint Superoxide fluxes limit nitric oxide-induced signaling
    Douglas D Thomas
    Tumor Biology Section, Radiation Biology Branch, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 281:25984-93. 2006
  4. pmc Hypoxic inducible factor 1alpha, extracellular signal-regulated kinase, and p53 are regulated by distinct threshold concentrations of nitric oxide
    Douglas D Thomas
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 101:8894-9. 2004
  5. ncbi request reprint The activation of metabolites of nitric oxide synthase by metals is both redox and oxygen dependent: a new feature of nitrogen oxide signaling
    Sonia Donzelli
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Antioxid Redox Signal 8:1363-71. 2006
  6. ncbi request reprint The chemical dynamics of NO and reactive nitrogen oxides: a practical guide
    Daniele Mancardi
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Curr Mol Med 4:723-40. 2004
  7. pmc A biochemical rationale for the discrete behavior of nitroxyl and nitric oxide in the cardiovascular system
    Katrina M Miranda
    Radiation Biology Branch, Building 10, Room B3 B69, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 100:9196-201. 2003
  8. ncbi request reprint Discriminating formation of HNO from other reactive nitrogen oxide species
    Sonia Donzelli
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 40:1056-66. 2006
  9. pmc Protein nitration is mediated by heme and free metals through Fenton-type chemistry: an alternative to the NO/O2- reaction
    Douglas D Thomas
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:12691-6. 2002
  10. ncbi request reprint Heme proteins and nitric oxide (NO): the neglected, eloquent chemistry in NO redox signaling and regulation
    Douglas D Thomas
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Antioxid Redox Signal 5:307-17. 2003

Research Grants

  1. Hypoxia controls nitric oxide-regulated proteins
    DOUGLAS THOMAS; Fiscal Year: 2007

Detail Information

Publications27

  1. pmc Molecular mechanisms for discrete nitric oxide levels in cancer
    Lisa A Ridnour
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Building 10, Bethesda, MD 20892, USA
    Nitric Oxide 19:73-6. 2008
    ..The quantification of redox events at the cellular level has revealed potential mechanisms that may either limit or potentiate tumor growth, and helped define the positive and negative function of nitric oxide in cancer...
  2. ncbi request reprint Asbestos redirects nitric oxide signaling through rapid catalytic conversion to nitrite
    Douglas D Thomas
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Cancer Res 66:11600-4. 2006
    ....
  3. ncbi request reprint Superoxide fluxes limit nitric oxide-induced signaling
    Douglas D Thomas
    Tumor Biology Section, Radiation Biology Branch, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 281:25984-93. 2006
    ..We conclude that the reaction of O2- with NO is an important regulatory mechanism, which modulates signaling pathways by limiting steady-state levels of NO and preventing H2O2 formation from O2-...
  4. pmc Hypoxic inducible factor 1alpha, extracellular signal-regulated kinase, and p53 are regulated by distinct threshold concentrations of nitric oxide
    Douglas D Thomas
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 101:8894-9. 2004
    ..The findings show that concentration and duration of NO exposure are critical determinants in the regulation of tumor-related proteins...
  5. ncbi request reprint The activation of metabolites of nitric oxide synthase by metals is both redox and oxygen dependent: a new feature of nitrogen oxide signaling
    Sonia Donzelli
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Antioxid Redox Signal 8:1363-71. 2006
    ..In this review, we discuss the biochemistry of such reactions in the context of their pharmacologic and biologic implications...
  6. ncbi request reprint The chemical dynamics of NO and reactive nitrogen oxides: a practical guide
    Daniele Mancardi
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Curr Mol Med 4:723-40. 2004
    ..From this discussion, a picture can be formed concerning the possible chemical dynamics, which can be plausible in different biological mechanisms...
  7. pmc A biochemical rationale for the discrete behavior of nitroxyl and nitric oxide in the cardiovascular system
    Katrina M Miranda
    Radiation Biology Branch, Building 10, Room B3 B69, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 100:9196-201. 2003
    ..The orthogonality of HNO and NO may be due to differential reactivity toward metals and thiols and in the cardiovascular system, may ultimately be driven by respective alteration of cAMP and cGMP levels...
  8. ncbi request reprint Discriminating formation of HNO from other reactive nitrogen oxide species
    Sonia Donzelli
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 40:1056-66. 2006
    ..These data indicate that free HNO can be biosynthesized and thus may function as an endogenous signaling agent that is regulated by GSH content...
  9. pmc Protein nitration is mediated by heme and free metals through Fenton-type chemistry: an alternative to the NO/O2- reaction
    Douglas D Thomas
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:12691-6. 2002
    ..Metal catalyzed nitration may account for the observed specificity of protein nitration seen under pathological conditions, suggesting a major role for translocated metals and the labilization of heme in NT formation...
  10. ncbi request reprint Heme proteins and nitric oxide (NO): the neglected, eloquent chemistry in NO redox signaling and regulation
    Douglas D Thomas
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Antioxid Redox Signal 5:307-17. 2003
    ..Here the basic chemistry of nitrosylation and the interactions of NO and other nitrogen oxides with metal-oxo species such as found in peroxidases and monoxygenases are discussed...
  11. doi request reprint Comparing the chemical biology of NO and HNO
    Wilmarie Flores-Santana
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Arch Pharm Res 32:1139-53. 2009
    ..In this review, we summarize some of the most important findings regarding NO and HNO chemistry, revealing some of the possible mechanisms of their beneficial actions...
  12. pmc Nitric oxide regulates matrix metalloproteinase-9 activity by guanylyl-cyclase-dependent and -independent pathways
    Lisa A Ridnour
    Radiation Biology Branch and Laboratory of Pathology, National Cancer Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 104:16898-903. 2007
    ....
  13. ncbi request reprint The chemistry of nitrosative stress induced by nitric oxide and reactive nitrogen oxide species. Putting perspective on stressful biological situations
    Lisa A Ridnour
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Biol Chem 385:1-10. 2004
    ....
  14. ncbi request reprint Further evidence for distinct reactive intermediates from nitroxyl and peroxynitrite: effects of buffer composition on the chemistry of Angeli's salt and synthetic peroxynitrite
    Katrina M Miranda
    Radiation Biology Branch, National Cancer Institute NIH, Building 10, Room B3 B69, Bethesda, MD 20892, USA
    Arch Biochem Biophys 401:134-44. 2002
    ..This study emphasizes the importance of buffer composition on chemical outcome and thus on interpretation and provides further evidence that ONOO(-) is not an intermediate formed between the reaction of O(2) and HNO produced by AS...
  15. ncbi request reprint Orthogonal properties of the redox siblings nitroxyl and nitric oxide in the cardiovascular system: a novel redox paradigm
    David A Wink
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bldg 10, Rm B3 B69, Bethesda, MD 20892, USA
    Am J Physiol Heart Circ Physiol 285:H2264-76. 2003
    ..This article discusses the emerging aspects of HNO chemistry and attempts to provide a framework for the distinct effects of NO and HNO in vivo...
  16. ncbi request reprint The biphasic nature of nitric oxide responses in tumor biology
    Lisa A Ridnour
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Antioxid Redox Signal 8:1329-37. 2006
    ..This article discusses the biphasic nature of NO in cancer biology and the implications of NO-driven redox flux for modulation of tumor-stimulated angiogenesis, growth, and metastasis...
  17. ncbi request reprint Guanylyl cyclase-dependent chemotaxis of endothelial cells in response to nitric oxide gradients
    Jeff S Isenberg
    Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 40:1028-33. 2006
    ..The findings suggest that subnanomolar NO gradients are sufficient to mobilize endothelial cell migration into hypoxic tissue during neovascularization events, such as in wound healing and cancer...
  18. ncbi request reprint Comparison of the reactivity of nitric oxide and nitroxyl with heme proteins. A chemical discussion of the differential biological effects of these redox related products of NOS
    Katrina M Miranda
    Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Building 10, Room B3 B69, Bethesda, MD 20892, USA
    J Inorg Biochem 93:52-60. 2003
    ....
  19. ncbi request reprint Guide for the use of nitric oxide (NO) donors as probes of the chemistry of NO and related redox species in biological systems
    Douglas D Thomas
    Tumor Biology Section, Radiation Biology Branch, National Institutes of Health National Cancer Institute, Bethesda, Maryland 20892, USA
    Methods Enzymol 359:84-105. 2002
  20. ncbi request reprint Ingress and reactive chemistry of nitroxyl-derived species within human cells
    Michael Graham Espey
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD20892, USA
    Free Radic Biol Med 33:827-34. 2002
    ..The substantial facilitation of oxygen-dependent nitroxyl chemistry by intact lipid bilayers supports a focusing role for the membrane in modulation of cellular constituents proteins by this unique species...
  21. pmc Focusing of nitric oxide mediated nitrosation and oxidative nitrosylation as a consequence of reaction with superoxide
    Michael G Espey
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:11127-32. 2002
    ..These modifications may be favored in microenvironments where the rate of O2- production is temporally and spatially contemporaneous with nitric oxide synthase activity, but not in excess of NO generation...
  22. ncbi request reprint A chemical perspective on the interplay between NO, reactive oxygen species, and reactive nitrogen oxide species
    Michael G Espey
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Ann N Y Acad Sci 962:195-206. 2002
    ..From this perspective of NO chemistry, the type, magnitude, location, and duration of either ROS or RNOS reactions may be predicted...
  23. pmc Nitric oxide regulates angiogenesis through a functional switch involving thrombospondin-1
    Lisa A Ridnour
    Radiation Biology Branch and Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:13147-52. 2005
    ..Limiting TSP1 expression by positive feedback through the ERK mitogen-activated protein kinase pathway may facilitate switching to a proangiogenic state at low doses of NO...
  24. ncbi request reprint Inflammation and IGF-I activate the Akt pathway in breast cancer
    Robyn L Prueitt
    Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Int J Cancer 120:796-805. 2007
    ..In summary, a proinflammatory environment was found to activate the Akt pathway in breast cancer, and may modify the association between the Akt phosphorylation status and breast cancer survival...
  25. pmc Direct real-time evaluation of nitration with green fluorescent protein in solution and within human cells reveals the impact of nitrogen dioxide vs. peroxynitrite mechanisms
    Michael Graham Espey
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:3481-6. 2002
    ..Taken together, these findings exemplified that heme-peroxidase-catalyzed formation of NO(2) may play a pivotal role in inflammatory and chronic disease settings while calling into question the significance of nitration by peroxynitrite...
  26. ncbi request reprint Mechanism of aerobic decomposition of Angeli's salt (sodium trioxodinitrate) at physiological pH
    Katrina M Miranda
    Department of Chemistry, University of Arizona, Tucson, Arizona 85721, USA
    J Am Chem Soc 127:722-31. 2005
    ..The mechanism of product autoxidation is also considered...
  27. ncbi request reprint Peroxynitrite and myocardial contractility: in vivo versus in vitro effects
    Tatsuo Katori
    935 Ross, Division of Cardiology, Department of Medicine, The Johns Hopkins Medical Institutions, 720 Rutland Avenue, Baltimore, MD 21205, USA
    Free Radic Biol Med 41:1606-18. 2006
    ..These data demonstrate that the site of generation within the cardiovascular system largely dictates the ability of ONOO- to directly or indirectly modulate cardiac pump function...

Research Grants1

  1. Hypoxia controls nitric oxide-regulated proteins
    DOUGLAS THOMAS; Fiscal Year: 2007
    ....