Research Topics
| Douglas D ThomasSummaryAffiliation: National Institutes of Health Country: USA Publications
Research Grants
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Detail Information
Publications
Molecular mechanisms for discrete nitric oxide levels in cancerLisa A Ridnour
Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Building 10, Bethesda, MD 20892, USA
Nitric Oxide 19:73-6. 2008..The quantification of redox events at the cellular level has revealed potential mechanisms that may either limit or potentiate tumor growth, and helped define the positive and negative function of nitric oxide in cancer...
Asbestos redirects nitric oxide signaling through rapid catalytic conversion to nitriteDouglas D Thomas
Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
Cancer Res 66:11600-4. 2006....- Superoxide fluxes limit nitric oxide-induced signalingDouglas D Thomas
Tumor Biology Section, Radiation Biology Branch, National Institutes of Health, Bethesda, MD 20892, USA
J Biol Chem 281:25984-93. 2006..We conclude that the reaction of O2- with NO is an important regulatory mechanism, which modulates signaling pathways by limiting steady-state levels of NO and preventing H2O2 formation from O2-... - Hypoxic inducible factor 1alpha, extracellular signal-regulated kinase, and p53 are regulated by distinct threshold concentrations of nitric oxideDouglas D Thomas
Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 101:8894-9. 2004..The findings show that concentration and duration of NO exposure are critical determinants in the regulation of tumor-related proteins... - The activation of metabolites of nitric oxide synthase by metals is both redox and oxygen dependent: a new feature of nitrogen oxide signalingSonia Donzelli
Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
Antioxid Redox Signal 8:1363-71. 2006..In this review, we discuss the biochemistry of such reactions in the context of their pharmacologic and biologic implications... - The chemical dynamics of NO and reactive nitrogen oxides: a practical guideDaniele Mancardi
Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
Curr Mol Med 4:723-40. 2004..From this discussion, a picture can be formed concerning the possible chemical dynamics, which can be plausible in different biological mechanisms... - A biochemical rationale for the discrete behavior of nitroxyl and nitric oxide in the cardiovascular systemKatrina M Miranda
Radiation Biology Branch, Building 10, Room B3 B69, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 100:9196-201. 2003..The orthogonality of HNO and NO may be due to differential reactivity toward metals and thiols and in the cardiovascular system, may ultimately be driven by respective alteration of cAMP and cGMP levels... - Discriminating formation of HNO from other reactive nitrogen oxide speciesSonia Donzelli
Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Free Radic Biol Med 40:1056-66. 2006..These data indicate that free HNO can be biosynthesized and thus may function as an endogenous signaling agent that is regulated by GSH content... - Protein nitration is mediated by heme and free metals through Fenton-type chemistry: an alternative to the NO/O2- reactionDouglas D Thomas
Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 99:12691-6. 2002..Metal catalyzed nitration may account for the observed specificity of protein nitration seen under pathological conditions, suggesting a major role for translocated metals and the labilization of heme in NT formation... - Heme proteins and nitric oxide (NO): the neglected, eloquent chemistry in NO redox signaling and regulationDouglas D Thomas
Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
Antioxid Redox Signal 5:307-17. 2003..Here the basic chemistry of nitrosylation and the interactions of NO and other nitrogen oxides with metal-oxo species such as found in peroxidases and monoxygenases are discussed... 
Comparing the chemical biology of NO and HNOWilmarie Flores-Santana
Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Arch Pharm Res 32:1139-53. 2009..In this review, we summarize some of the most important findings regarding NO and HNO chemistry, revealing some of the possible mechanisms of their beneficial actions...- Nitric oxide regulates matrix metalloproteinase-9 activity by guanylyl-cyclase-dependent and -independent pathwaysLisa A Ridnour
Radiation Biology Branch and Laboratory of Pathology, National Cancer Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 104:16898-903. 2007.... - The chemistry of nitrosative stress induced by nitric oxide and reactive nitrogen oxide species. Putting perspective on stressful biological situationsLisa A Ridnour
Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
Biol Chem 385:1-10. 2004.... - Further evidence for distinct reactive intermediates from nitroxyl and peroxynitrite: effects of buffer composition on the chemistry of Angeli's salt and synthetic peroxynitriteKatrina M Miranda
Radiation Biology Branch, National Cancer Institute NIH, Building 10, Room B3 B69, Bethesda, MD 20892, USA
Arch Biochem Biophys 401:134-44. 2002..This study emphasizes the importance of buffer composition on chemical outcome and thus on interpretation and provides further evidence that ONOO(-) is not an intermediate formed between the reaction of O(2) and HNO produced by AS... - Orthogonal properties of the redox siblings nitroxyl and nitric oxide in the cardiovascular system: a novel redox paradigmDavid A Wink
Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bldg 10, Rm B3 B69, Bethesda, MD 20892, USA
Am J Physiol Heart Circ Physiol 285:H2264-76. 2003..This article discusses the emerging aspects of HNO chemistry and attempts to provide a framework for the distinct effects of NO and HNO in vivo... - The biphasic nature of nitric oxide responses in tumor biologyLisa A Ridnour
Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
Antioxid Redox Signal 8:1329-37. 2006..This article discusses the biphasic nature of NO in cancer biology and the implications of NO-driven redox flux for modulation of tumor-stimulated angiogenesis, growth, and metastasis... - Guanylyl cyclase-dependent chemotaxis of endothelial cells in response to nitric oxide gradientsJeff S Isenberg
Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Free Radic Biol Med 40:1028-33. 2006..The findings suggest that subnanomolar NO gradients are sufficient to mobilize endothelial cell migration into hypoxic tissue during neovascularization events, such as in wound healing and cancer... - Comparison of the reactivity of nitric oxide and nitroxyl with heme proteins. A chemical discussion of the differential biological effects of these redox related products of NOSKatrina M Miranda
Tumor Biology Section, Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Building 10, Room B3-B69, Bethesda, MD 20892, USA
J Inorg Biochem 93:52-60. 2003.... - Guide for the use of nitric oxide (NO) donors as probes of the chemistry of NO and related redox species in biological systemsDouglas D Thomas
Tumor Biology Section, Radiation Biology Branch, National Institutes of Health/National Cancer Institute, Bethesda, Maryland 20892, USA
Methods Enzymol 359:84-105. 2002 - Ingress and reactive chemistry of nitroxyl-derived species within human cellsMichael Graham Espey
Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD20892, USA
Free Radic Biol Med 33:827-34. 2002..The substantial facilitation of oxygen-dependent nitroxyl chemistry by intact lipid bilayers supports a focusing role for the membrane in modulation of cellular constituents proteins by this unique species... - Focusing of nitric oxide mediated nitrosation and oxidative nitrosylation as a consequence of reaction with superoxideMichael G Espey
Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 99:11127-32. 2002..These modifications may be favored in microenvironments where the rate of O2- production is temporally and spatially contemporaneous with nitric oxide synthase activity, but not in excess of NO generation... - A chemical perspective on the interplay between NO, reactive oxygen species, and reactive nitrogen oxide speciesMichael G Espey
Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Ann N Y Acad Sci 962:195-206. 2002..From this perspective of NO chemistry, the type, magnitude, location, and duration of either ROS or RNOS reactions may be predicted... - Nitric oxide regulates angiogenesis through a functional switch involving thrombospondin-1Lisa A Ridnour
Radiation Biology Branch and Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 102:13147-52. 2005..Limiting TSP1 expression by positive feedback through the ERK mitogen-activated protein kinase pathway may facilitate switching to a proangiogenic state at low doses of NO... - Inflammation and IGF-I activate the Akt pathway in breast cancerRobyn L Prueitt
Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
Int J Cancer 120:796-805. 2007..In summary, a proinflammatory environment was found to activate the Akt pathway in breast cancer, and may modify the association between the Akt phosphorylation status and breast cancer survival... - Direct real-time evaluation of nitration with green fluorescent protein in solution and within human cells reveals the impact of nitrogen dioxide vs. peroxynitrite mechanismsMichael Graham Espey
Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 99:3481-6. 2002..Taken together, these findings exemplified that heme-peroxidase-catalyzed formation of NO(2) may play a pivotal role in inflammatory and chronic disease settings while calling into question the significance of nitration by peroxynitrite... - Mechanism of aerobic decomposition of Angeli's salt (sodium trioxodinitrate) at physiological pHKatrina M Miranda
Department of Chemistry, University of Arizona, Tucson, Arizona 85721, USA
J Am Chem Soc 127:722-31. 2005..The mechanism of product autoxidation is also considered... - Peroxynitrite and myocardial contractility: in vivo versus in vitro effectsTatsuo Katori
935 Ross, Division of Cardiology, Department of Medicine, The Johns Hopkins Medical Institutions, 720 Rutland Avenue, Baltimore, MD 21205, USA
Free Radic Biol Med 41:1606-18. 2006..These data demonstrate that the site of generation within the cardiovascular system largely dictates the ability of ONOO- to directly or indirectly modulate cardiac pump function...
Research Grants
- Hypoxia controls nitric oxide-regulated proteinsDOUGLAS THOMAS; Fiscal Year: 2007....