William Telford

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Small lasers in flow cytometry
    William G Telford
    Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 263:399-418. 2004
  2. ncbi request reprint Analysis of UV-excited fluorochromes by flow cytometry using near-ultraviolet laser diodes
    William G Telford
    Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    Cytometry A 61:9-17. 2004
  3. ncbi request reprint DPSS yellow-green 561-nm lasers for improved fluorochrome detection by flow cytometry
    William Telford
    Experimental Transplantation and Immunology Branch, Center for Cancer Research, NCI NIH, Bethesda, Maryland 20892, USA
    Cytometry A 68:36-44. 2005
  4. ncbi request reprint The minimal instrumentation requirements for Hoechst side population analysis: stem cell analysis on low-cost flow cytometry platforms
    Raquel Cabana
    NPE Systems, Pembroke Pines, Florida, USA
    Stem Cells 24:2573-81. 2006
  5. ncbi request reprint Side population analysis using a violet-excited cell-permeable DNA binding dye
    William G Telford
    Experimental Transplantation and Immunology Branch, National Cancer Institute, Building 10 Room 3 3297, 9000 Rockville Pike, Bethesda, Maryland 20892 USA
    Stem Cells 25:1029-36. 2007
  6. doi request reprint Stem cell identification by DyeCycle Violet side population analysis
    William G Telford
    Experimental Transplantation and Immunology Branch, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 946:163-79. 2013
  7. doi request reprint Flow cytometry of fluorescent proteins
    William G Telford
    Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Methods 57:318-30. 2012
  8. pmc Dynamics and mechanisms of quantum dot nanoparticle cellular uptake
    Yan Xiao
    Chemical Science and Technology Laboratory, National Institute of Standards and Technology NIST, Gaithersburg, MD, USA
    J Nanobiotechnology 8:13. 2010
  9. pmc Rapamycin delays growth of Wnt-1 tumors in spite of suppression of host immunity
    Elena V Svirshchevskaya
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho Maklaya Street 16 10, Moscow 117997, Moscow, Russia
    BMC Cancer 8:176. 2008
  10. ncbi request reprint Discrimination of the Hoechst side population in mouse bone marrow with violet and near-ultraviolet laser diodes
    William G Telford
    Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cytometry A 57:45-52. 2004

Collaborators

Detail Information

Publications37

  1. ncbi request reprint Small lasers in flow cytometry
    William G Telford
    Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 263:399-418. 2004
    ..Technical details and critical parameters for successful application of these lasers for biomedical analysis are reviewed...
  2. ncbi request reprint Analysis of UV-excited fluorochromes by flow cytometry using near-ultraviolet laser diodes
    William G Telford
    Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    Cytometry A 61:9-17. 2004
    ..In this study, the next generation of InGaN near-UV laser diodes (NUVLDs) emitting in the 370-375-nm range have been evaluated as laser sources for cuvette-based flow cytometers...
  3. ncbi request reprint DPSS yellow-green 561-nm lasers for improved fluorochrome detection by flow cytometry
    William Telford
    Experimental Transplantation and Immunology Branch, Center for Cancer Research, NCI NIH, Bethesda, Maryland 20892, USA
    Cytometry A 68:36-44. 2005
    ..In this study, we evaluate an even longer wavelength DPSS 561-nm for its ability to improve PE and DsRed fluorescent protein detection sensitivity...
  4. ncbi request reprint The minimal instrumentation requirements for Hoechst side population analysis: stem cell analysis on low-cost flow cytometry platforms
    Raquel Cabana
    NPE Systems, Pembroke Pines, Florida, USA
    Stem Cells 24:2573-81. 2006
    ..An inexpensive system of this type would make Hoechst SP analysis available to a much broader group of stem cell investigators...
  5. ncbi request reprint Side population analysis using a violet-excited cell-permeable DNA binding dye
    William G Telford
    Experimental Transplantation and Immunology Branch, National Cancer Institute, Building 10 Room 3 3297, 9000 Rockville Pike, Bethesda, Maryland 20892 USA
    Stem Cells 25:1029-36. 2007
    ..Substituting DCV for Hoechst 33342 in the SP technique may, therefore, allow side population analysis on flow cytometers with violet lasers...
  6. doi request reprint Stem cell identification by DyeCycle Violet side population analysis
    William G Telford
    Experimental Transplantation and Immunology Branch, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 946:163-79. 2013
    ..Critical parameters for good labeling, details on integrating simultaneous immunolabeling with DCV SP analysis, and proper data acquisition and analysis techniques are covered in detail...
  7. doi request reprint Flow cytometry of fluorescent proteins
    William G Telford
    Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Methods 57:318-30. 2012
    ....
  8. pmc Dynamics and mechanisms of quantum dot nanoparticle cellular uptake
    Yan Xiao
    Chemical Science and Technology Laboratory, National Institute of Standards and Technology NIST, Gaithersburg, MD, USA
    J Nanobiotechnology 8:13. 2010
    ..abstract:..
  9. pmc Rapamycin delays growth of Wnt-1 tumors in spite of suppression of host immunity
    Elena V Svirshchevskaya
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho Maklaya Street 16 10, Moscow 117997, Moscow, Russia
    BMC Cancer 8:176. 2008
    ..However, the role of Rapamycin-induced immune suppression on tumor progression has not been examined...
  10. ncbi request reprint Discrimination of the Hoechst side population in mouse bone marrow with violet and near-ultraviolet laser diodes
    William G Telford
    Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cytometry A 57:45-52. 2004
    ..In this study, we evaluated the ability of recently available violet and near-UV laser diodes to discriminate Hoechst SP on smaller cuvette-based flow cytometers...
  11. pmc Supercontinuum white light lasers for flow cytometry
    William G Telford
    Experimental Transplantation and Immunology Branch, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Cytometry A 75:450-9. 2009
    ..Almost any visible wavelength range can be made available for excitation, allowing access to virtually any fluorescent probe, and permitting "fine-tuning" of excitation wavelength to particular probes...
  12. ncbi request reprint Analysis of violet-excited fluorochromes by flow cytometry using a violet laser diode
    William G Telford
    Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cytometry A 54:48-55. 2003
    ....
  13. pmc Green fiber lasers: an alternative to traditional DPSS green lasers for flow cytometry
    William G Telford
    Experimental Transplantation and Immunology Branch, NCI NIH, Bethesda, Maryland 20892, USA
    Cytometry A 75:1031-9. 2009
    ..Fiber laser technology is, therefore, providing the flexibility necessary for precisely matching laser wavelengths to our flow cytometry applications...
  14. ncbi request reprint Telomere length measurement by fluorescence in situ hybridization and flow cytometry
    Veena Kapoor
    Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 263:385-98. 2004
    ..In this chapter, we describe the standard flow cytometric assay for measuring telomere length, including the incorporation of fluorochrome-conjugated antibody immunolabeling for measurement in cell subsets...
  15. pmc Single cell analysis of complex thymus stromal cell populations: rapid thymic epithelia preparation characterizes radiation injury
    Kirsten M Williams
    Experimental Transplantation and Immunology Branch, National Cancer Institute, NIH, Bethesda, Maryland, USA
    Clin Transl Sci 2:279-85. 2009
    ....
  16. pmc Exogenous insulin-like growth factor 1 enhances thymopoiesis predominantly through thymic epithelial cell expansion
    Yu Waye Chu
    Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Institutes of Health NIH, Bethesda, MD, USA
    Blood 112:2836-46. 2008
    ..These results indicate the critical point thymic function regulation by IGF-1 involves TEC expansion regulating thymocyte precursor entry and facilitating thymocyte development...
  17. ncbi request reprint Violet laser diodes in flow cytometry: an update
    William Telford
    Experimental Transplantation and Immunology Branch, Center for Cancer Research, NCI, NIH, Bethesda, Maryland 20892, USA
    Cytometry A 69:1153-60. 2006
    ..In this follow-up study, we have tested more powerful VLDs emitting at 55 mW, and a beam-merged dual module VLD with 100 mW combined output, for their ability to excite a variety of violet-excited fluorochromes, including CFP...
  18. ncbi request reprint Ex vivo rapamycin generates apoptosis-resistant donor Th2 cells that persist in vivo and prevent hemopoietic stem cell graft rejection
    Jacopo Mariotti
    Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 180:89-105. 2008
    ..We conclude that ex vivo rapamycin generates Th2 cells that are resistant to apoptosis, persist in vivo, and effectively prevent rejection by a mechanism that may be distinct from previously described graft-facilitating T cells...
  19. ncbi request reprint Detection of localized caspase activity in early apoptotic cells by laser scanning cytometry
    William G Telford
    Experimental Transplantation and Immunology Branch, Center for Cancer Research, NCI, NIH, Bethesda, Maryland 20892, USA
    Cytometry 47:81-8. 2002
    ..We describe a method utilizing maximum fluorescence pixel analysis by laser scanning cytometry (LSC) to detect early apoptotic cells...
  20. pmc Regulatory T cells and human myeloid dendritic cells promote tolerance via programmed death ligand-1
    Shoba Amarnath
    Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    PLoS Biol 8:e1000302. 2010
    ..This identification of a human Treg downstream cellular effector (DC) and molecular mechanism (PD-L1) will facilitate the rational design of clinical trials to modulate alloreactivity...
  21. pmc Quantum dots thermal stability improves simultaneous phenotype-specific telomere length measurement by FISH-flow cytometry
    Veena Kapoor
    Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol Methods 344:6-14. 2009
    ....
  22. ncbi request reprint Multiparametric analysis of apoptosis by flow and image cytometry
    William G Telford
    Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 263:141-60. 2004
    ..This multiparametric approach is also amenable to a variety of flow cytometric instrumentation, both old and new...
  23. pmc Dysregulation of IL-15-mediated T-cell homeostasis in TGF-beta dominant-negative receptor transgenic mice
    Philip J Lucas
    Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, CRC 3 3288, 10 Center Drive, Bethesda, MD 20892, USA
    Blood 108:2789-95. 2006
    ..These data establish TGF-beta as a critical and dominant tumor-suppressor pathway opposing IL-15-mediated CD8+ T-cell expansion and potential malignant transformation...
  24. doi request reprint Stem cell side population analysis and sorting using DyeCycle violet
    William G Telford
    National Cancer Institute, National Institutes of Health, Bethesda, Maryland
    Curr Protoc Cytom . 2010
    ..The procedure for DyeCycle Violet labeling for side population is described, as well as the limitations particular to this dye...
  25. ncbi request reprint Stem cell activity of human side population and alpha6 integrin-bright keratinocytes defined by a quantitative in vivo assay
    Atsushi Terunuma
    Dermatology Branch, National Cancer Institute NIH, Bldg 10 Room 12N260, 10 Center Drive MSC 1908, Bethesda, MD 20892 1908, USA
    Stem Cells 25:664-9. 2007
    ..These results definitively demonstrate that human alpha6-bright/CD71-dim keratinocytes are enriched with KSCs, whereas SP-KCs are not...
  26. ncbi request reprint CCR6 colocalizes with CD18 and enhances adhesion to activated endothelial cells in CCR6-transduced Jurkat T cells
    Wusi Maki
    Dermatology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    J Immunol 169:2346-53. 2002
    ..Clustering of CCR6 and coassociation with critical integrins may serve to strengthen adhesion between T cells and activated endothelial cells...
  27. doi request reprint Solid state yellow and orange lasers for flow cytometry
    Veena Kapoor
    Experimental Transplantation and Immunology Branch, NCI NIH, Bethesda, Maryland 20892, USA
    Cytometry A 73:570-7. 2008
    ..This technology fills an important gap in the laser wavelengths available for flow, now almost any fluorochrome requiring visible light excitation can be accommodated...
  28. ncbi request reprint Proliferation kinetics of subpopulations of human marrow cells determined by quantifying in vivo incorporation of [2H2]-glucose into DNA of S-phase cells
    Gretchen N Schwartz
    Experimental Transplantation and Immunology Branch, National Cancer Institute NIH, Bldg 10, Room 12S237, MSC 1907, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Blood 102:2068-73. 2003
    ..These studies are the first to demonstrate the use of in vivo [2H2]-glucose-SIMST to measure in vivo turnover kinetics of subpopulations of CD34+ cells and precursors in healthy human subjects...
  29. ncbi request reprint Histone deacetylase inhibitor pharmacodynamic analysis by multiparameter flow cytometry
    Eun Joo Chung
    Medical Oncology Clinical Research Unit, Bethesda, MD 20892, USA
    Ann Clin Lab Sci 35:397-406. 2005
    ..The technique described has significant advantages for the PD assessment of HDAC inhibitors as monotherapy and as a component of combination therapy trials...
  30. ncbi request reprint Side population keratinocytes resembling bone marrow side population stem cells are distinct from label-retaining keratinocyte stem cells
    Atsushi Terunuma
    Dermatology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 1908, USA
    J Invest Dermatol 121:1095-103. 2003
    ..Future in vivo studies will be required to analyze the function of side population keratinocytes in epidermal homeostasis and to determine if side population keratinocytes have characteristics of keratinocyte stem cells...
  31. ncbi request reprint Granzyme B activity in target cells detects attack by cytotoxic lymphocytes
    Beverly Z Packard
    OncoImmunin, Gaithersburg, MD 20877, USA
    J Immunol 179:3812-20. 2007
    ..Detection of target GzB activity followed by caspase 3 activation provides a unique readout of a potentially lethal injury delivered by cytotoxic lymphocytes...
  32. ncbi request reprint New lasers for flow cytometry: filling the gaps
    Veena Kapoor
    Nat Methods 4:678-9. 2007
  33. ncbi request reprint Translocation of cytoplasmic HSP70 onto the surface of EL-4 cells during apoptosis
    Alexander M Sapozhnikov
    Division of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia
    Cell Prolif 35:193-206. 2002
    ..Western blot analysis of HSP70 in conditioned supernatant obtained from EL-4 cell tissue showed that the observed decrease of HSP70 cell content might be due to surface HSP70 shedding into the intercellular space...
  34. ncbi request reprint Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates
    Judith E Berlier
    Molecular Probes, Inc, Eugene, Oregon 97402, USA
    J Histochem Cytochem 51:1699-712. 2003
    ....
  35. ncbi request reprint Semiconductor nanocrystal conjugates, FISH and pH
    Yan Xiao
    Nat Methods 2:723. 2005
  36. ncbi request reprint Tamoxifen and the farnesyl transferase inhibitor FTI-277 synergize to inhibit growth in estrogen receptor-positive breast tumor cell lines
    Chad A Ellis
    NCI, Department of Cell and Cancer Biology, Rockville, MD 20850 3300, USA
    Breast Cancer Res Treat 78:59-67. 2003
    ..Thus, tamoxifen and FTIs may synergize to promote apoptotic cell death in ER positive human breast tumor cells...
  37. ncbi request reprint Behavior of human foreskin keratinocytes expressing a hair follicle stem cell marker CD200
    Atsushi Terunuma
    J Invest Dermatol 128:1332-4. 2008