S Subramaniam

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Molecular architectures of trimeric SIV and HIV-1 envelope glycoproteins on intact viruses: strain-dependent variation in quaternary structure
    Tommi A White
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, United States of America
    PLoS Pathog 6:e1001249. 2010
  2. pmc Structural mechanism of trimeric HIV-1 envelope glycoprotein activation
    Erin E H Tran
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, United States of America
    PLoS Pathog 8:e1002797. 2012
  3. pmc Ion-abrasion scanning electron microscopy reveals surface-connected tubular conduits in HIV-infected macrophages
    Adam E Bennett
    Laboratory of Cell Biology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America
    PLoS Pathog 5:e1000591. 2009
  4. pmc Three-dimensional structures of soluble CD4-bound states of trimeric simian immunodeficiency virus envelope glycoproteins determined by using cryo-electron tomography
    Tommi A White
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    J Virol 85:12114-23. 2011
  5. ncbi request reprint Three-dimensional electron microscopy at molecular resolution
    Sriram Subramaniam
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    Annu Rev Biophys Biomol Struct 33:141-55. 2004
  6. pmc The SIV surface spike imaged by electron tomography: one leg or three?
    Sriram Subramaniam
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    PLoS Pathog 2:e91. 2006
  7. pmc Electron tomography of viruses
    Sriram Subramaniam
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, United States
    Curr Opin Struct Biol 17:596-602. 2007
  8. pmc Bridging the imaging gap: visualizing subcellular architecture with electron tomography
    Sriram Subramaniam
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health NIH Bethesda, MD 20892, USA
    Curr Opin Microbiol 8:316-22. 2005
  9. ncbi request reprint Crystallographic analysis of protein conformational changes in the bacteriorhodopsin photocycle
    S Subramaniam
    Laboratory of Biochemistry, National Cancer Institute, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1460:157-65. 2000
  10. ncbi request reprint Molecular mechanism of vectorial proton translocation by bacteriorhodopsin
    S Subramaniam
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 406:653-7. 2000

Collaborators

Detail Information

Publications45

  1. pmc Molecular architectures of trimeric SIV and HIV-1 envelope glycoproteins on intact viruses: strain-dependent variation in quaternary structure
    Tommi A White
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, United States of America
    PLoS Pathog 6:e1001249. 2010
    ....
  2. pmc Structural mechanism of trimeric HIV-1 envelope glycoprotein activation
    Erin E H Tran
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, United States of America
    PLoS Pathog 8:e1002797. 2012
    ..These findings suggest a new structural template for designing immunogens that can elicit antibodies targeting HIV at a vulnerable, pre-entry stage...
  3. pmc Ion-abrasion scanning electron microscopy reveals surface-connected tubular conduits in HIV-infected macrophages
    Adam E Bennett
    Laboratory of Cell Biology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland, United States of America
    PLoS Pathog 5:e1000591. 2009
    ..This potential mechanism for efficient virus trafficking between the cell surface and interior may represent a subversion of pre-existing vesicular machinery for antigen capture, processing, sequestration, and presentation...
  4. pmc Three-dimensional structures of soluble CD4-bound states of trimeric simian immunodeficiency virus envelope glycoproteins determined by using cryo-electron tomography
    Tommi A White
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    J Virol 85:12114-23. 2011
    ..These results provide new insights into the structural diversity of SIV Env and show that there are strain-dependent variations in the orientation of sCD4 bound to trimeric SIV Env...
  5. ncbi request reprint Three-dimensional electron microscopy at molecular resolution
    Sriram Subramaniam
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    Annu Rev Biophys Biomol Struct 33:141-55. 2004
    ..The rapid pace of development in this field suggests that comprehensive descriptions of the structures of whole cells and organelles in terms of the spatial arrangements of their molecular components may soon become routine...
  6. pmc The SIV surface spike imaged by electron tomography: one leg or three?
    Sriram Subramaniam
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    PLoS Pathog 2:e91. 2006
  7. pmc Electron tomography of viruses
    Sriram Subramaniam
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, United States
    Curr Opin Struct Biol 17:596-602. 2007
    ..Here, we review recent developments in both areas as they relate to our understanding of the biology of heterogeneous and pleiomorphic viruses...
  8. pmc Bridging the imaging gap: visualizing subcellular architecture with electron tomography
    Sriram Subramaniam
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health NIH Bethesda, MD 20892, USA
    Curr Opin Microbiol 8:316-22. 2005
    ....
  9. ncbi request reprint Crystallographic analysis of protein conformational changes in the bacteriorhodopsin photocycle
    S Subramaniam
    Laboratory of Biochemistry, National Cancer Institute, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1460:157-65. 2000
    ....
  10. ncbi request reprint Molecular mechanism of vectorial proton translocation by bacteriorhodopsin
    S Subramaniam
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 406:653-7. 2000
    ..2 A in-plane and 3.6 A vertical) resolution, is largely localized to helices F and G, and provides an 'opening' of the protein to protons on the cytoplasmic side of the membrane...
  11. ncbi request reprint Electron crystallography of bacteriorhodopsin with millisecond time resolution
    S Subramaniam
    Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20892, USA
    J Struct Biol 128:19-25. 1999
    ..These difference maps report on the nature and extent of light-induced conformational changes in the photocycle and have provided incisive tools for understanding the molecular mechanism of proton transport by bacteriorhodopsin...
  12. ncbi request reprint The structure of bacteriorhodopsin: an emerging consensus
    S Subramaniam
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    Curr Opin Struct Biol 9:462-8. 1999
    ....
  13. doi request reprint Infants of human immunodeficiency virus type 1-infected women in rural south India: feeding patterns and risk of mother-to-child transmission
    Jennifer S Read
    Pediatric, Adolescent, and Maternal AIDS Branch, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health Executive Building, Room 4B11C, 6100 Executive Boulevard MSC 7510, Bethesda, MD 20892 7510
    Pediatr Infect Dis J 29:14-7. 2010
    ..We assessed the infant feeding choices of HIV-1-infected women in rural Tamil Nadu, India, and risk factors for mother-to-child transmission of HIV-1...
  14. ncbi request reprint Integration of deletion mutants of bovine rhodopsin into the membrane of the endoplasmic reticulum
    J A Heymann
    National Cancer Institute, NIH, Laboratory of Biochemistry, Bethesda, MD 20892, USA
    Mol Membr Biol 17:165-74. 2000
    ....
  15. ncbi request reprint Automated data collection with a Tecnai 12 electron microscope: applications for molecular imaging by cryomicroscopy
    P Zhang
    Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20817, USA
    J Struct Biol 135:251-61. 2001
    ..Both features are implemented using Windows NT and Web-based tools and provide tools for automated data collection on any Tecnai microscope from any location...
  16. pmc Classification and 3D averaging with missing wedge correction in biological electron tomography
    A Bartesaghi
    Center for Cancer Research, National Institutes of Health, Building 50, Room 4306, Bethesda, MD 20892, USA
    J Struct Biol 162:436-50. 2008
    ....
  17. ncbi request reprint Factors determining proconvulsant and anticonvulsant effects of inhibitors of nitric oxide synthase in rodents
    R D Kirkby
    Neuronal Excitability Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892 1408, USA
    Epilepsy Res 24:91-100. 1996
    ..6)...
  18. pmc Three-dimensional imaging of the highly bent architecture of Bdellovibrio bacteriovorus by using cryo-electron tomography
    Mario J Borgnia
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, 50 South Drive, Bethesda, MD 20892, USA
    J Bacteriol 190:2588-96. 2008
    ..We propose that rearrangements of these structures, in combination with the unique properties of the cell envelope, may underlie the remarkable ability of B. bacteriovorus cells to find and enter bacterial prey...
  19. ncbi request reprint Cryoelectron tomographic analysis of an HIV-neutralizing protein and its complex with native viral gp120
    Adam Bennett
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 282:27754-9. 2007
    ....
  20. ncbi request reprint From structure to mechanism: electron crystallographic studies of bacteriorhodopsin
    Sriram Subramaniam
    Laboratory of Biochemistry, National Cancer Institute, National Instititutes of Health, Bethesda, MD 20817, USA
    Philos Transact A Math Phys Eng Sci 360:859-74. 2002
    ..Here, we focus on the structural basis for the function of this protein, with primary emphasis on the contributions of electron microscopy and crystallography towards unravelling the mechanism of vectorial proton pumping...
  21. ncbi request reprint Structural insights into the mechanism of proton pumping by bacteriorhodopsin
    Teruhisa Hirai
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20817, USA
    FEBS Lett 545:2-8. 2003
    ..Taken together with previous spectroscopic and biochemical investigations, these studies allow the description of a detailed molecular mechanism of the main steps in vectorial proton transport by bacteriorhodopsin...
  22. pmc Electron tomography in nanoparticle imaging and analysis
    Jeffrey S Lengyel
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Nanomedicine (Lond) 3:125-31. 2008
    ....
  23. pmc Molecular structure of a 9-MDa icosahedral pyruvate dehydrogenase subcomplex containing the E2 and E3 enzymes using cryoelectron microscopy
    Jacqueline L S Milne
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 281:4364-70. 2006
    ....
  24. ncbi request reprint A core-weighted fitting method for docking atomic structures into low-resolution maps: application to cryo-electron microscopy
    Xiongwu Wu
    Laboratory of Biophysical Chemistry, NHLBI, National Institutes of Health, Building 50, Room 3308, 50 South Drive, Bethesda, MD 20892, USA
    J Struct Biol 141:63-76. 2003
    ....
  25. pmc Molecular architecture and mechanism of an icosahedral pyruvate dehydrogenase complex: a multifunctional catalytic machine
    Jacqueline L S Milne
    Laboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    EMBO J 21:5587-98. 2002
    ....
  26. ncbi request reprint Three-dimensional structure of a bacterial oxalate transporter
    Teruhisa Hirai
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Struct Biol 9:597-600. 2002
    ..Overall, the architecture of the protein is remarkably symmetric, providing a compelling molecular explanation for the ability of such transporters to carry out bi-directional substrate transport...
  27. pmc Electron tomography of the contact between T cells and SIV/HIV-1: implications for viral entry
    Rachid Sougrat
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 3:e63. 2007
    ..Determination of the molecular composition and structure of the entry claw may facilitate the identification of improved drugs for the inhibition of HIV-1 entry...
  28. ncbi request reprint Electron tomography of bacterial chemotaxis receptor assemblies
    Peijun Zhang
    Laboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Methods Cell Biol 79:373-84. 2007
  29. pmc Three-dimensional electron microscopic imaging of membrane invaginations in Escherichia coli overproducing the chemotaxis receptor Tsr
    Jonathan Lefman
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20817, USA
    J Bacteriol 186:5052-61. 2004
    ..coli and that such receptor aggregates could play an important role in signal transduction during bacterial chemotaxis...
  30. pmc An energy-based three-dimensional segmentation approach for the quantitative interpretation of electron tomograms
    Alberto Bartesaghi
    Electrical and Computer Engineering Department, University of Minnesota, Minneapolis, MN 55455, USA
    IEEE Trans Image Process 14:1314-23. 2005
    ..It could also enable studies of much larger data sets, such as those which might be obtained from the tomographic analysis of HIV-infected cells from studies of large populations...
  31. ncbi request reprint Automated image acquisition and processing using a new generation of 4K x 4K CCD cameras for cryo electron microscopic studies of macromolecular assemblies
    Peijun Zhang
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Struct Biol 143:135-44. 2003
    ..8 MDa Bacillus stearothermophilus icosahedral pyruvate dehydrogenase complex, and its comparison with the previously reported atomic model for this complex obtained by X-ray crystallography...
  32. ncbi request reprint Projection structure of the bacterial oxalate transporter OxlT at 3.4A resolution
    Jurgen A W Heymann
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Struct Biol 144:320-6. 2003
    ....
  33. pmc Direct visualization of Escherichia coli chemotaxis receptor arrays using cryo-electron microscopy
    Peijun Zhang
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 104:3777-81. 2007
    ..Our results demonstrate that chemotaxis in E. coli requires the presence of chemoreceptor arrays and that the formation of these arrays requires the scaffolding interactions of the signaling molecules CheA and CheW...
  34. pmc Electron tomography of degenerating neurons in mice with abnormal regulation of iron metabolism
    Peijun Zhang
    Laboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, MD 20817, USA
    J Struct Biol 150:144-53. 2005
    ....
  35. pmc Structural model for 12-helix transporters belonging to the major facilitator superfamily
    Teruhisa Hirai
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Bacteriol 185:1712-8. 2003
    ....
  36. pmc Chemoreceptors in Caulobacter crescentus: trimers of receptor dimers in a partially ordered hexagonally packed array
    Cezar M Khursigara
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Bacteriol 190:6805-10. 2008
    ....
  37. pmc Molecular architecture of native HIV-1 gp120 trimers
    Jun Liu
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Nature 455:109-13. 2008
    ..Our findings elucidate the structure and conformational changes of trimeric HIV-1 gp120 relevant to antibody neutralization and attachment to target cells...
  38. pmc Electron microscopic analysis of membrane assemblies formed by the bacterial chemotaxis receptor Tsr
    Robert M Weis
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20817, USA
    J Bacteriol 185:3636-43. 2003
    ....
  39. pmc Site-specific 3D imaging of cells and tissues with a dual beam microscope
    Jurgen A W Heymann
    Laboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, MD, USA
    J Struct Biol 155:63-73. 2006
    ..Our results demonstrate that dual beam imaging is a powerful new tool for cellular and subcellular imaging in 3D for both basic biomedical and clinical applications...
  40. doi request reprint Extended polypeptide linkers establish the spatial architecture of a pyruvate dehydrogenase multienzyme complex
    Jeffrey S Lengyel
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Structure 16:93-103. 2008
    ....
  41. ncbi request reprint Stoichiometry and absolute quantification of proteins with mass spectrometry using fluorescent and isotope-labeled concatenated peptide standards
    Dhaval Nanavati
    Laboratory of Neurotoxicology, National Institute of Mental Health, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Proteomics 7:442-7. 2008
    ....
  42. pmc Automated 100-position specimen loader and image acquisition system for transmission electron microscopy
    Jonathan Lefman
    Laboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, MD 20817, USA
    J Struct Biol 158:318-26. 2007
    ....
  43. pmc Cell surface filaments of the gliding bacterium Flavobacterium johnsoniae revealed by cryo-electron tomography
    Jun Liu
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Bacteriol 189:7503-6. 2007
    ..These filaments are absent in cells of a nonmotile gldF mutant but are restored upon expression of plasmid-encoded GldF, a component of a putative ATP-binding cassette transporter...
  44. pmc Structure and transport mechanism of the bacterial oxalate transporter OxlT
    Teruhisa Hirai
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biophys J 87:3600-7. 2004
    ....