Research Topics
Genomes and GenesSpecies | S SubramaniamSummaryAffiliation: National Institutes of Health Country: USA Publications
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Detail Information
Publications
Three-dimensional structures of soluble CD4-bound states of trimeric simian immunodeficiency virus envelope glycoproteins determined by using cryo-electron tomographyTommi A White
Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
J Virol 85:12114-23. 2011..These results provide new insights into the structural diversity of SIV Env and show that there are strain-dependent variations in the orientation of sCD4 bound to trimeric SIV Env...- Bridging the imaging gap: visualizing subcellular architecture with electron tomographySriram Subramaniam
Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health NIH Bethesda, MD 20892, USA
Curr Opin Microbiol 8:316-22. 2005.... 
Molecular mechanism of vectorial proton translocation by bacteriorhodopsinS Subramaniam
MRC Laboratory of Molecular Biology, Cambridge, UK
Nature 406:653-7. 2000..2 A in-plane and 3.6 A vertical) resolution, is largely localized to helices F and G, and provides an 'opening' of the protein to protons on the cytoplasmic side of the membrane...- Crystallographic analysis of protein conformational changes in the bacteriorhodopsin photocycleS Subramaniam
Laboratory of Biochemistry, National Cancer Institute, Bethesda, MD 20892, USA
Biochim Biophys Acta 1460:157-65. 2000.... - Three-dimensional electron microscopy at molecular resolutionSriram Subramaniam
Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
Annu Rev Biophys Biomol Struct 33:141-55. 2004..The rapid pace of development in this field suggests that comprehensive descriptions of the structures of whole cells and organelles in terms of the spatial arrangements of their molecular components may soon become routine... - Electron tomography of virusesSriram Subramaniam
Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, United States
Curr Opin Struct Biol 17:596-602. 2007..Here, we review recent developments in both areas as they relate to our understanding of the biology of heterogeneous and pleiomorphic viruses... - The SIV surface spike imaged by electron tomography: one leg or three?Sriram Subramaniam
Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
PLoS Pathog 2:e91. 2006 - Electron crystallography of bacteriorhodopsin with millisecond time resolutionS Subramaniam
Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20892, USA
J Struct Biol 128:19-25. 1999..These difference maps report on the nature and extent of light-induced conformational changes in the photocycle and have provided incisive tools for understanding the molecular mechanism of proton transport by bacteriorhodopsin... - The structure of bacteriorhodopsin: an emerging consensusS Subramaniam
MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
Curr Opin Struct Biol 9:462-8. 1999.... - Factors determining proconvulsant and anticonvulsant effects of inhibitors of nitric oxide synthase in rodentsR D Kirkby
Neuronal Excitability Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892 1408, USA
Epilepsy Res 24:91-100. 1996..6)... 
Infants of human immunodeficiency virus type 1-infected women in rural south India: feeding patterns and risk of mother-to-child transmissionJennifer S Read
Pediatric, Adolescent, and Maternal AIDS Branch, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health Executive Building, Room 4B11C, 6100 Executive Boulevard MSC 7510, Bethesda, MD 20892 7510
Pediatr Infect Dis J 29:14-7. 2010..We assessed the infant feeding choices of HIV-1-infected women in rural Tamil Nadu, India, and risk factors for mother-to-child transmission of HIV-1...- Integration of deletion mutants of bovine rhodopsin into the membrane of the endoplasmic reticulumJ A Heymann
National Cancer Institute, NIH, Laboratory of Biochemistry, Bethesda, MD 20892, USA
Mol Membr Biol 17:165-74. 2000.... - Automated data collection with a Tecnai 12 electron microscope: applications for molecular imaging by cryomicroscopyP Zhang
Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20817, USA
J Struct Biol 135:251-61. 2001..Both features are implemented using Windows NT and Web-based tools and provide tools for automated data collection on any Tecnai microscope from any location... - Classification and 3D averaging with missing wedge correction in biological electron tomographyA Bartesaghi
Center for Cancer Research, National Institutes of Health, Building 50, Room 4306, Bethesda, MD 20892, USA
J Struct Biol 162:436-50. 2008.... - Molecular structure of a 9-MDa icosahedral pyruvate dehydrogenase subcomplex containing the E2 and E3 enzymes using cryoelectron microscopyJacqueline L S Milne
Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, MD 20892, USA
J Biol Chem 281:4364-70. 2006.... - Electron tomography of bacterial chemotaxis receptor assembliesPeijun Zhang
Laboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
Methods Cell Biol 79:373-84. 2007 - Electron tomography of the contact between T cells and SIV/HIV-1: implications for viral entryRachid Sougrat
Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
PLoS Pathog 3:e63. 2007..Determination of the molecular composition and structure of the entry claw may facilitate the identification of improved drugs for the inhibition of HIV-1 entry... - Cryoelectron tomographic analysis of an HIV-neutralizing protein and its complex with native viral gp120Adam Bennett
Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 282:27754-9. 2007.... - Three-dimensional imaging of the highly bent architecture of Bdellovibrio bacteriovorus by using cryo-electron tomographyMario J Borgnia
Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, 50 South Drive, Bethesda, MD 20892, USA
J Bacteriol 190:2588-96. 2008..We propose that rearrangements of these structures, in combination with the unique properties of the cell envelope, may underlie the remarkable ability of B. bacteriovorus cells to find and enter bacterial prey... - Electron tomography in nanoparticle imaging and analysisJeffrey S Lengyel
Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
Nanomedicine (Lond) 3:125-31. 2008.... - An energy-based three-dimensional segmentation approach for the quantitative interpretation of electron tomogramsAlberto Bartesaghi
Electrical and Computer Engineering Department, University of Minnesota, Minneapolis, MN 55455, USA
IEEE Trans Image Process 14:1314-23. 2005..It could also enable studies of much larger data sets, such as those which might be obtained from the tomographic analysis of HIV-infected cells from studies of large populations... - Projection structure of the bacterial oxalate transporter OxlT at 3.4A resolutionJurgen A W Heymann
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Struct Biol 144:320-6. 2003.... - Structural insights into the mechanism of proton pumping by bacteriorhodopsinTeruhisa Hirai
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20817, USA
FEBS Lett 545:2-8. 2003..Taken together with previous spectroscopic and biochemical investigations, these studies allow the description of a detailed molecular mechanism of the main steps in vectorial proton transport by bacteriorhodopsin... - From structure to mechanism: electron crystallographic studies of bacteriorhodopsinSriram Subramaniam
Laboratory of Biochemistry, National Cancer Institute, National Instititutes of Health, Bethesda, MD 20817, USA
Philos Transact A Math Phys Eng Sci 360:859-74. 2002..Here, we focus on the structural basis for the function of this protein, with primary emphasis on the contributions of electron microscopy and crystallography towards unravelling the mechanism of vectorial proton pumping... - Automated image acquisition and processing using a new generation of 4K x 4K CCD cameras for cryo electron microscopic studies of macromolecular assembliesPeijun Zhang
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Struct Biol 143:135-44. 2003..8 MDa Bacillus stearothermophilus icosahedral pyruvate dehydrogenase complex, and its comparison with the previously reported atomic model for this complex obtained by X-ray crystallography... - A core-weighted fitting method for docking atomic structures into low-resolution maps: application to cryo-electron microscopyXiongwu Wu
Laboratory of Biophysical Chemistry, NHLBI, National Institutes of Health, Building 50, Room 3308, 50 South Drive, Bethesda, MD 20892, USA
J Struct Biol 141:63-76. 2003.... - Three-dimensional electron microscopic imaging of membrane invaginations in Escherichia coli overproducing the chemotaxis receptor TsrJonathan Lefman
Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20817, USA
J Bacteriol 186:5052-61. 2004..coli and that such receptor aggregates could play an important role in signal transduction during bacterial chemotaxis... - Molecular architecture and mechanism of an icosahedral pyruvate dehydrogenase complex: a multifunctional catalytic machineJacqueline L S Milne
Laboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, MD 20892, USA
EMBO J 21:5587-98. 2002.... - Three-dimensional structure of a bacterial oxalate transporterTeruhisa Hirai
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Nat Struct Biol 9:597-600. 2002..Overall, the architecture of the protein is remarkably symmetric, providing a compelling molecular explanation for the ability of such transporters to carry out bi-directional substrate transport... - Molecular architecture of native HIV-1 gp120 trimersJun Liu
Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
Nature 455:109-13. 2008..Our findings elucidate the structure and conformational changes of trimeric HIV-1 gp120 relevant to antibody neutralization and attachment to target cells... - Chemoreceptors in Caulobacter crescentus: trimers of receptor dimers in a partially ordered hexagonally packed arrayCezar M Khursigara
Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Bacteriol 190:6805-10. 2008.... - Structural model for 12-helix transporters belonging to the major facilitator superfamilyTeruhisa Hirai
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
J Bacteriol 185:1712-8. 2003.... - Extended polypeptide linkers establish the spatial architecture of a pyruvate dehydrogenase multienzyme complexJeffrey S Lengyel
Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Structure 16:93-103. 2008.... - Stoichiometry and absolute quantification of proteins with mass spectrometry using fluorescent and isotope-labeled concatenated peptide standardsDhaval Nanavati
Laboratory of Neurotoxicology, National Institute of Mental Health, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Cell Proteomics 7:442-7. 2008.... - Electron microscopic analysis of membrane assemblies formed by the bacterial chemotaxis receptor TsrRobert M Weis
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20817, USA
J Bacteriol 185:3636-43. 2003.... - Direct visualization of Escherichia coli chemotaxis receptor arrays using cryo-electron microscopyPeijun Zhang
Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 104:3777-81. 2007..Our results demonstrate that chemotaxis in E. coli requires the presence of chemoreceptor arrays and that the formation of these arrays requires the scaffolding interactions of the signaling molecules CheA and CheW... - Automated 100-position specimen loader and image acquisition system for transmission electron microscopyJonathan Lefman
Laboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, MD 20817, USA
J Struct Biol 158:318-26. 2007.... - Structure and transport mechanism of the bacterial oxalate transporter OxlTTeruhisa Hirai
Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Biophys J 87:3600-7. 2004.... - Site-specific 3D imaging of cells and tissues with a dual beam microscopeJurgen A W Heymann
Laboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, MD, USA
J Struct Biol 155:63-73. 2006..Our results demonstrate that dual beam imaging is a powerful new tool for cellular and subcellular imaging in 3D for both basic biomedical and clinical applications... - Electron tomography of degenerating neurons in mice with abnormal regulation of iron metabolismPeijun Zhang
Laboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, MD 20817, USA
J Struct Biol 150:144-53. 2005.... - Cell surface filaments of the gliding bacterium Flavobacterium johnsoniae revealed by cryo-electron tomographyJun Liu
Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Bacteriol 189:7503-6. 2007..These filaments are absent in cells of a nonmotile gldF mutant but are restored upon expression of plasmid-encoded GldF, a component of a putative ATP-binding cassette transporter...