Kanta Subbarao


Affiliation: National Institutes of Health
Country: USA


  1. Fabozzi G, Oler A, Liu P, Chen Y, Mindaye S, Dolan M, et al. Strand-Specific Dual RNA Sequencing of Bronchial Epithelial Cells Infected with Influenza A/H3N2 Viruses Reveals Splicing of Gene Segment 6 and Novel Host-Virus Interactions. J Virol. 2018;92: pubmed publisher
    ..We report novel elements that differ between seasonal and laboratory strains highlighting the complexity of the host-virus interplay at the RNA level. ..
  2. Broadbent A, Santos C, Godbout R, Subbarao K. The temperature-sensitive and attenuation phenotypes conferred by mutations in the influenza virus PB2, PB1, and NP genes are influenced by the species of origin of the PB2 gene in reassortant viruses derived from influenza A/California/07/2009 and A. J Virol. 2014;88:12339-47 pubmed publisher
    ..Our study provides information that could benefit the rational design of alternative LAIV backbones against animal origin influenza viruses. ..
  3. Moore I, Lamirande E, Paskel M, Donahue D, Kenney H, Qin J, et al. Severity of clinical disease and pathology in ferrets experimentally infected with influenza viruses is influenced by inoculum volume. J Virol. 2014;88:13879-91 pubmed publisher
    ..These data highlight some less explored features of the model, methods of experimental infection, and clinical disease outcomes in a research setting. ..
  4. O Donnell C, Vogel L, Matsuoka Y, Jin H, Subbarao K. The matrix gene segment destabilizes the acid and thermal stability of the hemagglutinin of pandemic live attenuated influenza virus vaccines. J Virol. 2014;88:12374-84 pubmed publisher
    ..The reduced HA stability and temperature sensitivity of the pLAIV viruses may account for their restricted replication in clinical trials. ..
  5. Gretebeck L, Subbarao K. Animal models for SARS and MERS coronaviruses. Curr Opin Virol. 2015;13:123-9 pubmed publisher
    ..In this review, we present factors to consider in establishing an animal model for the study of novel coronaviruses and compare the different animal models that have been employed to study SARS-CoV and MERS-CoV. ..
  6. Czakó R, Vogel L, Sutton T, Matsuoka Y, Krammer F, Chen Z, et al. H5N2 vaccine viruses on Russian and US live attenuated influenza virus backbones demonstrate similar infectivity, immunogenicity and protection in ferrets. Vaccine. 2018;36:1871-1879 pubmed publisher
    ..These observations suggest that differences in clinical performance of H5 pLAIVs may be due to factors other than inherent biological properties of the two MDVs. ..
  7. Broadbent A, Santos C, Anafu A, Wimmer E, Mueller S, Subbarao K. Evaluation of the attenuation, immunogenicity, and efficacy of a live virus vaccine generated by codon-pair bias de-optimization of the 2009 pandemic H1N1 influenza virus, in ferrets. Vaccine. 2016;34:563-570 pubmed publisher
    ..Taken together, our data demonstrate the potential of using CPBD technology for the development of a live influenza virus vaccine if the level of attenuation is optimized. ..
  8. Houser K, Subbarao K. Influenza vaccines: challenges and solutions. Cell Host Microbe. 2015;17:295-300 pubmed publisher
    ..This review focuses on how currently licensed influenza vaccines are generated in the U.S., why the biology of influenza poses vaccine challenges, and vaccine approaches on the horizon that address these challenges. ..
  9. Broadbent A, Santos C, Paskel M, Matsuoka Y, Lu J, Chen Z, et al. Replication of live attenuated cold-adapted H2N2 influenza virus vaccine candidates in non human primates. Vaccine. 2015;33:193-200 pubmed publisher
    ..We suggest that CMs and RMs may be the preferred species for evaluating H2N2 LAIV viruses, and the TEC67 ca virus may be the most promising H2N2 LAIV candidate for further evaluation. ..

More Information


  1. Baz M, Paskel M, Matsuoka Y, Zengel J, Cheng X, Treanor J, et al. A live attenuated equine H3N8 influenza vaccine is highly immunogenic and efficacious in mice and ferrets. J Virol. 2015;89:1652-9 pubmed publisher
    ..We generated a live attenuated H3N8 vaccine candidate and demonstrated that the vaccine was immunogenic and protected mice and ferrets against homologous and heterologous EIV. ..
  2. Sutton T, Subbarao K. Development of animal models against emerging coronaviruses: From SARS to MERS coronavirus. Virology. 2015;479-480:247-58 pubmed publisher
  3. Baz M, Paskel M, Matsuoka Y, Zengel J, Cheng X, Treanor J, et al. A Single Dose of an Avian H3N8 Influenza Virus Vaccine Is Highly Immunogenic and Efficacious against a Recently Emerged Seal Influenza Virus in Mice and Ferrets. J Virol. 2015;89:6907-17 pubmed publisher
    ..Therefore, we generated a live attenuated avian H3N8 vaccine candidate and demonstrated that a single dose of the vaccine was highly immunogenic and protected mice and ferrets against homologous and heterologous H3N8 avian viruses. ..
  4. request reprint
    Subbarao K, Roberts A. Is there an ideal animal model for SARS?. Trends Microbiol. 2006;14:299-303 pubmed
    ..Here, we suggest aspects of an ideal animal model for studies of SARS pathogenesis and vaccine development and present our assessment of the strengths and limitations of the current animal models for SARS. ..
  5. Baz M, Boonnak K, Paskel M, Santos C, Powell T, Townsend A, et al. Nonreplicating influenza A virus vaccines confer broad protection against lethal challenge. MBio. 2015;6:e01487-15 pubmed publisher
  6. Subbarao K, Matsuoka Y. The prospects and challenges of universal vaccines for influenza. Trends Microbiol. 2013;21:350-8 pubmed publisher
    ..Several recent scientific advances have generated renewed enthusiasm and hope for universal vaccines that will induce broad protection from a range of influenza viruses. ..
  7. Paules C, Subbarao K. Influenza. Lancet. 2017;390:697-708 pubmed publisher
    ..We also review the animal-human interface of influenza, with a focus on current pandemic threats. ..
  8. request reprint
    Lakdawala S, Fodor E, Subbarao K. Moving On Out: Transport and Packaging of Influenza Viral RNA into Virions. Annu Rev Virol. 2016;3:411-427 pubmed
  9. Boonnak K, Matsuoka Y, Wang W, Suguitan A, Chen Z, Paskel M, et al. Development of Clade-Specific and Broadly Reactive Live Attenuated Influenza Virus Vaccines against Rapidly Evolving H5 Subtype Viruses. J Virol. 2017;91: pubmed publisher
    ..Our study permits a comparison of the two approaches, and the data support the further development of both vaccine viruses to optimally prepare for the further spread of clade 2.2.1 or viruses. ..
  10. Jegaskanda S, Mason R, Andrews S, Wheatley A, Zhang R, Reynoso G, et al. Intranasal Live Influenza Vaccine Priming Elicits Localized B Cell Responses in Mediastinal Lymph Nodes. J Virol. 2018;92: pubmed publisher
  11. Sutton T, Lamirande E, Czakó R, Subbarao K. Evaluation of the Biological Properties and Cross-Reactive Antibody Response to H10 Influenza Viruses in Ferrets. J Virol. 2017;91: pubmed publisher
    ..By evaluating the cross-reactive antibody response to the H10 viruses and combining this analysis with viral replication and weight loss findings, we identified A/mallard/Portugal/79906/2009 (H10N7) as a suitable H10 vaccine strain. ..
  12. Sutton T, Lamirande E, Bock K, Moore I, Koudstaal W, Rehman M, et al. In Vitro Neutralization Is Not Predictive of Prophylactic Efficacy of Broadly Neutralizing Monoclonal Antibodies CR6261 and CR9114 against Lethal H2 Influenza Virus Challenge in Mice. J Virol. 2017;91: pubmed publisher
    ..These findings emphasize the importance of in vivo evaluation and testing of bNAbs. ..