G Storz

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs
    Aixia Zhang
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Mol Cell 9:11-22. 2002
  2. ncbi request reprint The SufE protein and the SufBCD complex enhance SufS cysteine desulfurase activity as part of a sulfur transfer pathway for Fe-S cluster assembly in Escherichia coli
    F Wayne Outten
    Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 278:45713-9. 2003
  3. pmc An antisense RNA controls synthesis of an SOS-induced toxin evolved from an antitoxin
    Mitsuoki Kawano
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Microbiol 64:738-54. 2007
  4. pmc Discovery of Fur binding site clusters in Escherichia coli by information theory models
    Zehua Chen
    National Cancer Institute at Frederick, Center for Cancer Research Nanobiology Program, Basic Research Program, SAIC Frederick, Inc, Frederick, MD 21702 1201, USA
    Nucleic Acids Res 35:6762-77. 2007
  5. pmc Detection of 5'- and 3'-UTR-derived small RNAs and cis-encoded antisense RNAs in Escherichia coli
    Mitsuoki Kawano
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development Building 18T, Room 101, Bethesda, MD 20892 5430, USA
    Nucleic Acids Res 33:1040-50. 2005
  6. pmc Abundance of type I toxin-antitoxin systems in bacteria: searches for new candidates and discovery of novel families
    Elizabeth M Fozo
    Eunice Kennedy Shriver National Institute of Child Health and Human Development and National Center for Biotechnology Information, National Institutes of Health, Bethesda, MD 20894, USA
    Nucleic Acids Res 38:3743-59. 2010
  7. ncbi request reprint Controlling mRNA stability and translation with small, noncoding RNAs
    Gisela Storz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 5430, USA
    Curr Opin Microbiol 7:140-4. 2004
  8. ncbi request reprint An abundance of RNA regulators
    Gisela Storz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Biochem 74:199-217. 2005
  9. ncbi request reprint An expanding universe of noncoding RNAs
    Gisela Storz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 296:1260-3. 2002
  10. pmc Regulation by small RNAs in bacteria: expanding frontiers
    Gisela Storz
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, 18 Library Drive, Bethesda, MD 20892 5430, USA
    Mol Cell 43:880-91. 2011

Collaborators

Detail Information

Publications48

  1. ncbi request reprint The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs
    Aixia Zhang
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Mol Cell 9:11-22. 2002
    ..We also show that Hfq increases the OxyS RNA interaction with its target messages and propose that the enhancement of RNA-RNA pairing may be a general function of Hfq, Sm, and Sm-like proteins...
  2. ncbi request reprint The SufE protein and the SufBCD complex enhance SufS cysteine desulfurase activity as part of a sulfur transfer pathway for Fe-S cluster assembly in Escherichia coli
    F Wayne Outten
    Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 278:45713-9. 2003
    ..We propose that this sulfur transfer mechanism may be important for limiting sulfide release during oxidative stress conditions in vivo...
  3. pmc An antisense RNA controls synthesis of an SOS-induced toxin evolved from an antitoxin
    Mitsuoki Kawano
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Microbiol 64:738-54. 2007
    ..We suggest that SymE promotion of RNA cleavage may be important for the recycling of RNAs damaged under SOS-inducing conditions...
  4. pmc Discovery of Fur binding site clusters in Escherichia coli by information theory models
    Zehua Chen
    National Cancer Institute at Frederick, Center for Cancer Research Nanobiology Program, Basic Research Program, SAIC Frederick, Inc, Frederick, MD 21702 1201, USA
    Nucleic Acids Res 35:6762-77. 2007
    ..The Pseudomonas aeruginosa and Bacillus subtilis Fur models are highly similar to the E. coli Fur model, suggesting that the Fur-DNA recognition mechanism may be conserved for even distantly related bacteria...
  5. pmc Detection of 5'- and 3'-UTR-derived small RNAs and cis-encoded antisense RNAs in Escherichia coli
    Mitsuoki Kawano
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development Building 18T, Room 101, Bethesda, MD 20892 5430, USA
    Nucleic Acids Res 33:1040-50. 2005
    ..In addition, we identified a few small RNAs that are expressed opposite protein-coding genes and could base pair with 5' or 3' ends of the mRNAs with perfect complementarity...
  6. pmc Abundance of type I toxin-antitoxin systems in bacteria: searches for new candidates and discovery of novel families
    Elizabeth M Fozo
    Eunice Kennedy Shriver National Institute of Child Health and Human Development and National Center for Biotechnology Information, National Institutes of Health, Bethesda, MD 20894, USA
    Nucleic Acids Res 38:3743-59. 2010
    ..Our results suggest that type I toxin-antitoxin modules are much more widely distributed among bacteria than previously appreciated...
  7. ncbi request reprint Controlling mRNA stability and translation with small, noncoding RNAs
    Gisela Storz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 5430, USA
    Curr Opin Microbiol 7:140-4. 2004
    ..Here we summarize the physiological roles of the basepairing RNAs, examine their prevalence in bacteria and discuss unresolved questions regarding their mechanisms of action...
  8. ncbi request reprint An abundance of RNA regulators
    Gisela Storz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Biochem 74:199-217. 2005
    ..Other regulatory RNAs act by modifying protein activity, in some cases by mimicking the structures of other RNA or DNA molecules...
  9. ncbi request reprint An expanding universe of noncoding RNAs
    Gisela Storz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 296:1260-3. 2002
    ..These findings raise several fundamental questions: How many ncRNAs are encoded by a genome? Given the absence of a diagnostic open reading frame, how can these genes be identified? How can all the functions of ncRNAs be elucidated?..
  10. pmc Regulation by small RNAs in bacteria: expanding frontiers
    Gisela Storz
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, 18 Library Drive, Bethesda, MD 20892 5430, USA
    Mol Cell 43:880-91. 2011
    ..Most importantly, recent studies have served to clarify the abundance of remaining questions about how, when, and why sRNA-mediated regulation is of such importance to bacterial lifestyles...
  11. ncbi request reprint Oxidative stress
    G Storz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Opin Microbiol 2:188-94. 1999
    ..These studies have also shown that the oxidative stress responses are intimately coupled to other regulatory networks in the cell...
  12. pmc Mutational analysis of the redox-sensitive transcriptional regulator OxyR: regions important for DNA binding and multimerization
    I Kullik
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892
    J Bacteriol 177:1285-91. 1995
    ....
  13. ncbi request reprint A small, stable RNA induced by oxidative stress: role as a pleiotropic regulator and antimutator
    S Altuvia
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell 90:43-53. 1997
    ..Our results suggest that the oxyS RNA acts as a regulator that integrates adaptation to hydrogen peroxide with other cellular stress responses and helps to protect cells against oxidative damage...
  14. pmc DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide
    M Zheng
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Bacteriol 183:4562-70. 2001
    ..These results expand our understanding of the oxidative stress response and raise interesting questions regarding the nature of other regulators that modulate gene expression in response to hydrogen peroxide...
  15. ncbi request reprint Activation of the OxyR transcription factor by reversible disulfide bond formation
    M Zheng
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 279:1718-21. 1998
    ..The redox potential of OxyR was determined to be -185 millivolts, ensuring that OxyR is reduced in the absence of stress. These results represent an example of redox signaling through disulfide bond formation and reduction...
  16. pmc The OxyS regulatory RNA represses rpoS translation and binds the Hfq (HF-I) protein
    A Zhang
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 17:6061-8. 1998
    ..Co-immunoprecipitation and gel mobility shift experiments revealed that the OxyS RNA binds Hfq, suggesting that OxyS represses rpoS translation by altering Hfq activity...
  17. ncbi request reprint Redox-dependent shift of OxyR-DNA contacts along an extended DNA-binding site: a mechanism for differential promoter selection
    M B Toledano
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
    Cell 78:897-909. 1994
    ....
  18. pmc The Escherichia coli OxyS regulatory RNA represses fhlA translation by blocking ribosome binding
    S Altuvia
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 17:6069-75. 1998
    ..We examined the mechanism of OxyS repression of fhlA and found that the OxyS RNA inhibits fhlA translation by pairing with a short sequence overlapping the Shine-Dalgarno sequence, thereby blocking ribosome binding/translation...
  19. pmc Computation-directed identification of OxyR DNA binding sites in Escherichia coli
    M Zheng
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Bacteriol 183:4571-9. 2001
    ..These results demonstrate the versatile modes of regulation by OxyR and illustrate the need to learn more about the ensembles of binding sites and transcripts in the E. coli genome...
  20. ncbi request reprint Redox sensing by prokaryotic transcription factors
    M Zheng
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Biochem Pharmacol 59:1-6. 2000
    ..The mechanisms by which these and other prokaryotic proteins sense redox signals have provided useful paradigms for understanding redox signal transduction in eukaryotic cells...
  21. pmc OxyR and SoxRS regulation of fur
    M Zheng
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Bacteriol 181:4639-43. 1999
    ..These results demonstrate that iron metabolism is coordinately regulated with the oxidative stress defenses...
  22. ncbi request reprint The dps promoter is activated by OxyR during growth and by IHF and sigma S in stationary phase
    S Altuvia
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
    Mol Microbiol 13:265-72. 1994
    ..Our results suggest that the dps promoter is recognized by both sigma 70-holoenzyme and sigma S-holoenzyme, since OxyR acts through sigma 70 and the starts of the OxyR- and sigma S-dependent transcripts are identical...
  23. pmc Identification of novel small RNAs using comparative genomics and microarrays
    K M Wassarman
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 15:1637-51. 2001
    ..The approaches used here should allow identification of small RNAs in other organisms...
  24. pmc Mutational analysis of the redox-sensitive transcriptional regulator OxyR: regions important for oxidation and transcriptional activation
    I Kullik
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892
    J Bacteriol 177:1275-84. 1995
    ..Unexpectedly, RNA polymerase was also found to reciprocally affect OxyR binding...
  25. ncbi request reprint Small RNAs in Escherichia coli
    K M Wassarman
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 5430, USA
    Trends Microbiol 7:37-45. 1999
    ....
  26. ncbi request reprint The OxyR regulon
    G Storz
    Laboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892
    Antonie Van Leeuwenhoek 58:157-61. 1990
    ....
  27. ncbi request reprint Roles of the glutathione- and thioredoxin-dependent reduction systems in the Escherichia coli and saccharomyces cerevisiae responses to oxidative stress
    O Carmel-Harel
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Microbiol 54:439-61. 2000
    ..coli OxyR and SoxR and the S. cerevisiae Yap1p transcriptional regulators of the adaptive responses to oxidative stress...
  28. ncbi request reprint Regulating bacterial transcription with small RNAs
    G Storz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892 5430, USA
    Cold Spring Harb Symp Quant Biol 71:269-73. 2006
    ..Other small RNAs affect transcription termination via base-pairing interactions with sequences in the mRNA. Here, we discuss current understanding and questions regarding the roles of small RNAs in regulating transcription...
  29. pmc Effects of peroxides on susceptibilities of Escherichia coli and Mycobacterium smegmatis to isoniazid
    J L Rosner
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD 20892
    Antimicrob Agents Chemother 38:1829-33. 1994
    ..Our results suggest that INH is converted to a more active drug by reaction with peroxides and that the INH susceptibilities of enterobacteria and mycobacteria are mechanistically related...
  30. ncbi request reprint Role of thioredoxin reductase in the Yap1p-dependent response to oxidative stress in Saccharomyces cerevisiae
    O Carmel Harel
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 5430, USA
    Mol Microbiol 39:595-605. 2001
    ....
  31. ncbi request reprint 6S RNA regulates E. coli RNA polymerase activity
    K M Wassarman
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell 101:613-23. 2000
    ..We show 6S RNA represses expression from a sigma70-dependent promoter during stationary phase. Our results suggest that the interaction of 6S RNA with RNA polymerase modulates sigma70-holoenzyme activity...
  32. pmc Cloning and sequencing of thiol-specific antioxidant from mammalian brain: alkyl hydroperoxide reductase and thiol-specific antioxidant define a large family of antioxidant enzymes
    H Z Chae
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 91:7017-21. 1994
    ..We also report that a second family of proteins, defined by the 57-kDa component (AhpF) of alkyl hydroperoxide reductase and by thioredoxin reductase, has expanded to include six additional members...
  33. ncbi request reprint Bacterial defenses against oxidative stress
    G Storz
    Laboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892
    Trends Genet 6:363-8. 1990
    ....
  34. pmc The Escherichia coli MntR miniregulon includes genes encoding a small protein and an efflux pump required for manganese homeostasis
    Lauren S Waters
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, Maryland, USA
    J Bacteriol 193:5887-97. 2011
    ..Deletion of mntP leads to profound manganese sensitivity and to elevated intracellular manganese levels. This work thus defines two new proteins involved in manganese homeostasis and suggests mechanisms for their action...
  35. pmc Detection of low-level promoter activity within open reading frame sequences of Escherichia coli
    Mitsuoki Kawano
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Building 18T, Room 101, Bethesda, MD 20892 5430, USA
    Nucleic Acids Res 33:6268-76. 2005
    ..coli chromosome carries numerous -35 and -10 sequences with weak promoter activity but that most are not productively expressed because other features needed to enhance promoter activity and transcript stability are absent...
  36. pmc MicC, a second small-RNA regulator of Omp protein expression in Escherichia coli
    Shuo Chen
    Ibis Therapeutics, Isis Pharmaceuticals, Inc, Carlsbad, California, USA
    J Bacteriol 186:6689-97. 2004
    ..We suggest that the MicF and MicC RNAs act in conjunction with the EnvZ-OmpR two-component system to control the OmpF/OmpC protein ratio in response to a variety of environmental stimuli...
  37. ncbi request reprint Global analysis of small RNA and mRNA targets of Hfq
    Aixia Zhang
    Cell Biology and Metabolism Branch, National Institute of Child Health and Development, Bethesda MD 20892, USA
    Mol Microbiol 50:1111-24. 2003
    ..Thus Hfq immunoprecipitation followed by microarray analysis is a highly effective method for detecting a major class of small RNAs as well as identifying new Hfq functions...
  38. pmc An antibody-based microarray assay for small RNA detection
    Zonglin Hu
    Bacterial Toxins and Therapeutics Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Nucleic Acids Res 34:e52. 2006
    ..25 fmol (i.e. concentration of 10 pM in a 25 microl reaction). The method is an efficient, robust and inexpensive technique that allows quantitative analysis of gene expression and does not discriminate against short or structured RNAs...
  39. pmc GadY, a small-RNA regulator of acid response genes in Escherichia coli
    Jason A Opdyke
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Bacteriol 186:6698-705. 2004
    ....
  40. pmc Target prediction for small, noncoding RNAs in bacteria
    Brian Tjaden
    Computer Science Department, Wellesley College, Wellesley, MA 02481, USA
    Nucleic Acids Res 34:2791-802. 2006
    ..Our results show that TargetRNA is a useful tool for finding mRNA targets of sRNAs, although its rate of success varies between sRNAs...
  41. ncbi request reprint Modulating the outer membrane with small RNAs
    Maude Guillier
    Laboratory of Molecular Biology, National Cancer Institute, Bethesda 20892, Maryland, USA
    Genes Dev 20:2338-48. 2006
    ..Here we review what is known about these sRNAs and discuss the implications of this regulation...
  42. pmc Prominent roles of the NorR and Fur regulators in the Escherichia coli transcriptional response to reactive nitrogen species
    Partha Mukhopadhyay
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 101:745-50. 2004
    ....
  43. ncbi request reprint Oxygen, metabolism, and gene expression: the T-Rex connection
    Matthew J Wood
    Structure 13:2-4. 2005
  44. pmc Exploiting thiol modifications
    Patricia J Kiley
    Department of Biomolecular Chemistry, University of Wisconsin Medical School in Madison, Wisconsin, USA
    PLoS Biol 2:e400. 2004
  45. ncbi request reprint Redox regulation of OxyR requires specific disulfide bond formation involving a rapid kinetic reaction path
    Cheolju Lee
    Center for Cellular Switch Protein Structure, Korea Research Institute of Bioscience and Biotechnology, 52 Euh eun dong, Yuseong Gu, Daejeon 305 806, Korea
    Nat Struct Mol Biol 11:1179-85. 2004
    ..On the basis of these observations we conclude that OxyR activation requires specific disulfide bond formation and that the rapid kinetic reaction path and conformation strain, respectively, drive the oxidation and reduction of OxyR...
  46. ncbi request reprint Take your vitamins with a pinch of RNA
    Robert A Weisberg
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Mol Cell 10:1266-8. 2002
    ....
  47. ncbi request reprint A suf operon requirement for Fe-S cluster assembly during iron starvation in Escherichia coli
    F Wayne Outten
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Mol Microbiol 52:861-72. 2004
    ..coli, whereas the suf operon is specifically adapted to synthesize Fe-S clusters when iron or sulphur metabolism is disrupted by iron starvation or oxidative stress...
  48. pmc Mutational analysis to define an activating region on the redox-sensitive transcriptional regulator OxyR
    Xunde Wang
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892 5430, USA
    J Bacteriol 188:8335-42. 2006
    ..The two mutations, D142A and R273H, giving the most dramatic phenotype are located in a patch on the surface of the oxidized OxyR protein and possibly define an activating region on OxyR...