Alasdair C Steven

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Structure, assembly, and antigenicity of hepatitis B virus capsid proteins
    Alasdair C Steven
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Adv Virus Res 64:125-64. 2005
  2. ncbi request reprint Biochemistry. Viral glycoproteins and an evolutionary conundrum
    Alasdair C Steven
    Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, 50 South Drive, Bethesda, MD 20892, USA
    Science 313:177-8. 2006
  3. pmc Structural changes in Influenza virus at low pH characterized by cryo-electron tomography
    Juan Fontana
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland, USA
    J Virol 86:2919-29. 2012
  4. pmc Allosteric signaling and a nuclear exit strategy: binding of UL25/UL17 heterodimers to DNA-Filled HSV-1 capsids
    Benes L Trus
    Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 26:479-89. 2007
  5. pmc Visualization of the two-step fusion process of the retrovirus avian sarcoma/leukosis virus by cryo-electron tomography
    Giovanni Cardone
    Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland, USA
    J Virol 86:12129-37. 2012
  6. ncbi request reprint ClpA and ClpX ATPases bind simultaneously to opposite ends of ClpP peptidase to form active hybrid complexes
    Joaquin Ortega
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Bethesda, MD 20892, USA
    J Struct Biol 146:217-26. 2004
  7. pmc Visualization of the herpes simplex virus portal in situ by cryo-electron tomography
    Giovanni Cardone
    Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892 8025, USA
    Virology 361:426-34. 2007
  8. ncbi request reprint Architecture of Ure2p prion filaments: the N-terminal domains form a central core fiber
    Ulrich Baxa
    Laboratories of Structural Biology, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, and Biochemistry and Genetics, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 278:43717-27. 2003
  9. pmc Packaging accessory protein P7 and polymerase P2 have mutually occluding binding sites inside the bacteriophage 6 procapsid
    Daniel Nemecek
    Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland, USA
    J Virol 86:11616-24. 2012
  10. pmc Local and global mobility in the ClpA AAA+ chaperone detected by cryo-electron microscopy: functional connotations
    Grégory Effantin
    Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Structure 18:553-62. 2010

Detail Information

Publications96

  1. ncbi request reprint Structure, assembly, and antigenicity of hepatitis B virus capsid proteins
    Alasdair C Steven
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Adv Virus Res 64:125-64. 2005
  2. ncbi request reprint Biochemistry. Viral glycoproteins and an evolutionary conundrum
    Alasdair C Steven
    Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, 50 South Drive, Bethesda, MD 20892, USA
    Science 313:177-8. 2006
  3. pmc Structural changes in Influenza virus at low pH characterized by cryo-electron tomography
    Juan Fontana
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland, USA
    J Virol 86:2919-29. 2012
    ..However, it appears desirable for productive infection that fusion should proceed before the RNPs become coagulated with matrix protein, as eventually happens at low pH...
  4. pmc Allosteric signaling and a nuclear exit strategy: binding of UL25/UL17 heterodimers to DNA-Filled HSV-1 capsids
    Benes L Trus
    Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 26:479-89. 2007
    ..We infer that binding of the ordered population reflects structural changes induced on the outer surface as pressure builds up inside the capsid during DNA packaging. Its binding may signal that the C-capsid is ready to exit the nucleus...
  5. pmc Visualization of the two-step fusion process of the retrovirus avian sarcoma/leukosis virus by cryo-electron tomography
    Giovanni Cardone
    Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland, USA
    J Virol 86:12129-37. 2012
    ..In addition, some of the products presented a density layer underlying and resolved from the viral membrane, which may represent detachment of the matrix protein to facilitate the fusion process...
  6. ncbi request reprint ClpA and ClpX ATPases bind simultaneously to opposite ends of ClpP peptidase to form active hybrid complexes
    Joaquin Ortega
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Bethesda, MD 20892, USA
    J Struct Biol 146:217-26. 2004
    ..Thus, ClpXAP is a bifunctional protease whose two ends can independently target different classes of substrates...
  7. pmc Visualization of the herpes simplex virus portal in situ by cryo-electron tomography
    Giovanni Cardone
    Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892 8025, USA
    Virology 361:426-34. 2007
    ..This distinction may be indicative of divergence at the level of portal-related functions other than its role as a DNA channel...
  8. ncbi request reprint Architecture of Ure2p prion filaments: the N-terminal domains form a central core fiber
    Ulrich Baxa
    Laboratories of Structural Biology, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, and Biochemistry and Genetics, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 278:43717-27. 2003
    ....
  9. pmc Packaging accessory protein P7 and polymerase P2 have mutually occluding binding sites inside the bacteriophage 6 procapsid
    Daniel Nemecek
    Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland, USA
    J Virol 86:11616-24. 2012
    ..Thus, P7 may promote packaging either by interacting directly with incoming RNA or by modulating the structure of the translocation pore...
  10. pmc Local and global mobility in the ClpA AAA+ chaperone detected by cryo-electron microscopy: functional connotations
    Grégory Effantin
    Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Structure 18:553-62. 2010
    ..Based on these observations, we present a pseudo-atomic model of ClpAP holoenzyme, a dynamic proteolytic nanomachine...
  11. pmc Molecular basis for the high degree of antigenic cross-reactivity between hepatitis B virus capsids (HBcAg) and dimeric capsid-related protein (HBeAg): insights into the enigmatic nature of the e-antigen
    Norman R Watts
    Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Mol Biol 398:530-41. 2010
    ..These results suggest new approaches for the isolation of the authentic e-antigen, its biological assay, and its stabilization as an immune complex for structural studies...
  12. pmc A free energy cascade with locks drives assembly and maturation of bacteriophage HK97 capsid
    Philip D Ross
    Laboratory of Molecular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, MD 20892, USA
    J Mol Biol 364:512-25. 2006
    ..To explain the whiffleball phenotype, we suggest that these effects propagate to the capsomer periphery in such a way as to differentially affect the stability or solubility of dissociated pentamers, leaving only hexamers to reassemble...
  13. pmc The UL36 tegument protein of herpes simplex virus 1 has a composite binding site at the capsid vertices
    Giovanni Cardone
    Laboratory of Structural Biology, National Institutes of Health, Bethesda, Maryland, USA
    J Virol 86:4058-64. 2012
    ..They also indicate how sequential conformational changes in the maturing nucleocapsid control the ordered binding, first of UL25/UL17 and then of UL36...
  14. pmc Suppression of a morphogenic mutant in Rous sarcoma virus capsid protein by a second-site mutation: a cryoelectron tomography study
    Carmen Butan
    Laboratory of Structural Biology, National Institute for Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda Maryland 20892, USA
    J Virol 84:6377-86. 2010
    ..We conclude that the wild-type CA protein sequence represents an evolutionary compromise between competing requirements for optimization of Gag assembly (of the immature virion) and CA assembly (in the maturing virion)...
  15. pmc Specificity of an anti-capsid antibody associated with Hepatitis B Virus-related acute liver failure
    Weimin Wu
    Laboratory of Structural Biology, National Institute for Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, United States
    J Struct Biol 181:53-60. 2013
    ....
  16. pmc Procapsid assembly, maturation, nuclear exit: dynamic steps in the production of infectious herpesvirions
    Giovanni Cardone
    National Institute for Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Adv Exp Med Biol 726:423-39. 2012
    ....
  17. ncbi request reprint Dynamics of herpes simplex virus capsid maturation visualized by time-lapse cryo-electron microscopy
    J Bernard Heymann
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Struct Biol 10:334-41. 2003
    ..The primary mechanism underlying maturation is relative rotations of domains of VP5, the major capsid protein...
  18. ncbi request reprint Filaments of the Ure2p prion protein have a cross-beta core structure
    Ulrich Baxa
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Struct Biol 150:170-9. 2005
    ..Local area electron diffraction and X-ray diffraction from partially aligned specimens showed that the 4.7A reflection is meridional and therefore the underlying structure is cross-beta...
  19. pmc Role of the propeptide in controlling conformation and assembly state of hepatitis B virus e-antigen
    Norman R Watts
    Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Mol Biol 409:202-13. 2011
    ..However, this intercalation distorts the dimer into an assembly-reluctant conformation...
  20. pmc Crosslinking renders bacteriophage HK97 capsid maturation irreversible and effects an essential stabilization
    Philip D Ross
    Laboratory of Molecular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, MD, USA
    EMBO J 24:1352-63. 2005
    ..At pH 4, most K169Y capsids remain as EI-II, whereas wild-type capsids proceed to EI-III, suggesting that crosslink formation drives maturation by a Brownian ratchet mechanism...
  21. pmc RSV capsid polymorphism correlates with polymerization efficiency and envelope glycoprotein content: implications that nucleation controls morphogenesis
    Carmen Butan
    Laboratory of Structural Biology, National Institute for Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Mol Biol 376:1168-81. 2008
    ..These observations imply that initiation of CA assembly, in which interactions of spike endodomains with the Gag layer play a role, is a critical determinant of core morphology...
  22. ncbi request reprint The N-terminal substrate-binding domain of ClpA unfoldase is highly mobile and extends axially from the distal surface of ClpAP protease
    Takashi Ishikawa
    Laboratory of Structural Biology Research, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Bethesda, MD 20892 8025, USA
    J Struct Biol 146:180-8. 2004
    ....
  23. pmc Generation and characterization of a chimeric rabbit/human Fab for co-crystallization of HIV-1 Rev
    Stephen J Stahl
    Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892 2775, USA
    J Mol Biol 397:697-708. 2010
    ..The corresponding single-chain antibody (scFv) was also prepared, offering the potential of intracellular antibody therapeutics against human immunodeficiency virus type 1...
  24. ncbi request reprint Characterization of beta-sheet structure in Ure2p1-89 yeast prion fibrils by solid-state nuclear magnetic resonance
    Ulrich Baxa
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892 8025, USA
    Biochemistry 46:13149-62. 2007
    ....
  25. pmc Non-canonical binding of an antibody resembling a naïve B cell receptor immunoglobulin to hepatitis B virus capsids
    Norman R Watts
    Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Mol Biol 379:1119-29. 2008
    ..Considerations of conserved sequence motifs in other such molecules suggest that other naïve B cells may interact with HBV capsids in much the same way...
  26. pmc Cryo-EM study of Hepatitis B virus core antigen capsids decorated with antibodies from a human patient
    Eaazhisai Kandiah
    Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Struct Biol 177:145-51. 2012
    ..These results show that epitopes on the floor, far (~30 Å) from the immunodominant loop, are clinically relevant and that murine anti-cAg antibodies afford a good model for the human system...
  27. pmc Binding of the ClpA unfoldase opens the axial gate of ClpP peptidase
    Grégory Effantin
    Laboratory of Structural Biology Research, NIAMS, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 285:14834-40. 2010
    ..Based on these observations, we propose that access to the ClpP degradation chamber is controlled allosterically by hinged movements of its N-terminal loops, which the symmetry-mismatched binding of ClpA suffices to induce...
  28. pmc Stepwise expansion of the bacteriophage ϕ6 procapsid: possible packaging intermediates
    Daniel Nemecek
    National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Mol Biol 414:260-71. 2011
    ....
  29. pmc Virus maturation: dynamics and mechanism of a stabilizing structural transition that leads to infectivity
    Alasdair C Steven
    Laboratory of Structural Biology Research, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Opin Struct Biol 15:227-36. 2005
    ..Moreover, it has been possible to visualize maturation at the submolecular level in movies based on time-resolved cryo-electron microscopy...
  30. pmc Visualization of a missing link in retrovirus capsid assembly
    Giovanni Cardone
    Laboratory of Structural Biology, National Institute for Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 457:694-8. 2009
    ....
  31. ncbi request reprint Three-dimensional structure of herpes simplex virus from cryo-electron tomography
    Kay Grünewald
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Science 302:1396-8. 2003
    ..The envelope contained 600 to 750 glycoprotein spikes that varied in length, spacing, and in the angles at which they emerge from the membrane. Their distribution was nonrandom, suggesting functional clustering...
  32. ncbi request reprint Is the prion domain of soluble Ure2p unstructured?
    Michael M Pierce
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892 0830, USA
    Biochemistry 44:321-8. 2005
    ..These results suggest that the N-terminal prion domain is unstructured in the soluble protein and does not have a specific interaction with the C-terminus...
  33. pmc Subunit Folds and Maturation Pathway of a dsRNA Virus Capsid
    Daniel Nemecek
    National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, 50 South Drive, Bethesda, MD 20892, USA Central European Institute of Technology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic
    Structure 21:1374-83. 2013
    ..The capsid structure and its stepwise maturation that is coupled to sequential packaging of three RNA segments sets the cystoviruses apart from other dsRNA viruses as a dynamic molecular machine. ..
  34. pmc Structural engineering of a phage lysin that targets gram-negative pathogens
    Petra Lukacik
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 109:9857-62. 2012
    ....
  35. pmc Structural dependence of HET-s amyloid fibril infectivity assessed by cryoelectron microscopy
    Naoko Mizuno
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 108:3252-7. 2011
    ..In triplets, this surface is occluded, blocking nucleation and thereby explaining their lack of infectivity...
  36. pmc Poliovirus 2C protein forms homo-oligomeric structures required for ATPase activity
    Peter Adams
    NIAID, National Institutes of Health, Bethesda, MD 20892 8011, USA
    J Biol Chem 284:22012-21. 2009
    ..Finally, deletion of the N-terminal 38 amino acids blocked oligomerization of the fusion protein and eliminated ATPase activity, despite retention of an unaltered nucleotide-binding domain...
  37. ncbi request reprint A resolution criterion for electron tomography based on cross-validation
    Giovanni Cardone
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Struct Biol 151:117-29. 2005
    ..Applicable to entire tomograms or selected structures, NLOO has also been tested on experimental tomographic data...
  38. pmc Structure and polymorphism of the UL6 portal protein of herpes simplex virus type 1
    Benes L Trus
    Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Building 50, Room 1517, MSC 8025, 50 South Drive, Bethesda, MD 20892 8025, USA
    J Virol 78:12668-71. 2004
    ..Its architecture resembles those of bacteriophage portal/connector proteins...
  39. pmc Functional architecture of the retromer cargo-recognition complex
    Aitor Hierro
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, US Department of Health and Human Services, Bethesda, Maryland 20892, USA
    Nature 449:1063-7. 2007
    ..This extended structure presents multiple binding sites for the SNX complex and receptor cargo, and appears capable of flexing to conform to curved vesicular membranes...
  40. ncbi request reprint Prions of Saccharomyces and Podospora
    Ulrich Baxa
    Laboratory of Biochemistry, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    Contrib Microbiol 11:50-71. 2004
  41. pmc Electron microscopic evidence in support of alpha-solenoid models of proteasomal subunits Rpn1 and Rpn2
    Grégory Effantin
    Laboratory of Structural Biology Research, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Mol Biol 386:1204-11. 2009
    ..The results support the alpha-solenoid model, except that they indicate that the repeats are organized not as symmetrical circular toroids but in less regular horseshoe-like structures...
  42. ncbi request reprint Co-assembly of envoplakin and periplakin into oligomers and Ca(2+)-dependent vesicle binding: implications for cornified cell envelope formation in stratified squamous epithelia
    Andrey E Kalinin
    Laboratory of Skin Biology and Laboratory of Structural Biology, NIAMS, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:22773-80. 2004
    ....
  43. pmc Computational resources for cryo-electron tomography in Bsoft
    J Bernard Heymann
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Building 50, Room 1515, 50 South Drive MSC 8025, Bethesda, MD 20892 8025, USA
    J Struct Biol 161:232-42. 2008
    ..Resources are also included that allow resolution assessment by cross-validation (NLOO2D); denoising and interpretation; and the extraction, mutual alignment, and averaging of tomographic sub-volumes...
  44. pmc HIV-1 maturation inhibitor bevirimat stabilizes the immature Gag lattice
    Paul W Keller
    Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, NIH, Building 50, Room 1517, 50 South Drive, MSC 8025, Bethesda, MD 20892, USA
    J Virol 85:1420-8. 2011
    ..In both cases, the observed failure to assemble mature capsids correlates with the loss of infectivity...
  45. pmc Cryo-electron tomography of bacteriophage phi6 procapsids shows random occupancy of the binding sites for RNA polymerase and packaging NTPase
    Daniel Nemecek
    National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, 50 South Dr, Bethesda, MD 20892, USA
    J Struct Biol 171:389-96. 2010
    ..These observations indicate that although P2 and P4 act sequentially on the same substrates there is no direct physical coupling between their activities...
  46. pmc Initial location of the RNA-dependent RNA polymerase in the bacteriophage Phi6 procapsid determined by cryo-electron microscopy
    Anindito Sen
    Laboratory of Structural Biology Research, NIAMS, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 283:12227-31. 2008
    ..We propose that, during maturation, the P2 molecules rotate to occupy positions closer to adjacent 5-fold vertices where they conduct replication and transcription...
  47. pmc Arrangement of L2 within the papillomavirus capsid
    Christopher B Buck
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 5624, USA
    J Virol 82:5190-7. 2008
    ..This structural information should facilitate investigation of L2 function during the assembly and entry phases of the papillomavirus life cycle...
  48. pmc Multiple modes of endophilin-mediated conversion of lipid vesicles into coated tubes: implications for synaptic endocytosis
    Naoko Mizuno
    Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 285:23351-8. 2010
    ..Our findings suggest that the adaptability of endophilin-lipid interactions underlies dynamic changes of endocytic membranes...
  49. ncbi request reprint A second symmetry mismatch at the portal vertex of bacteriophage T7: 8-fold symmetry in the procapsid core
    Mario E Cerritelli
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, MSC 8025, Bethesda, MD 20892 8025, USA
    J Mol Biol 327:1-6. 2003
    ..We have investigated the core structure by cryo-electron microscopy and image analysis of procapsids and find that it observes 8-fold symmetry. Stoichiometry data indicate that its major constituent is an octamer of gp15...
  50. ncbi request reprint Molecular mechanisms in bacteriophage T7 procapsid assembly, maturation, and DNA containment
    Mario E Cerritelli
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Adv Protein Chem 64:301-23. 2003
    ..In the mature T7 head, the DNA is organized as a tightly wound coaxial spool, with the DNA coiled around the core in at least four and perhaps as many as six concentric shells...
  51. ncbi request reprint Molecular dynamics of protein complexes from four-dimensional cryo-electron microscopy
    J Bernard Heymann
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Struct Biol 147:291-301. 2004
    ....
  52. ncbi request reprint The N-terminal prion domain of Ure2p converts from an unfolded to a thermally resistant conformation upon filament formation
    Ulrich Baxa
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Mol Biol 339:259-64. 2004
    ..In contrast, no thermal signal was associated with the N-terminal domains: in the soluble state of Ure2p, because they are unfolded; in the filamentous state, because their robust amyloid conformation resists heating to 100 degrees C...
  53. pmc Antigenic switching of hepatitis B virus by alternative dimerization of the capsid protein
    Michael A DiMattia
    Division of Structural Biology, Henry Wellcome Building for Genomic Medicine, University of Oxford, Roosevelt Drive, Headington OX3 7BN, UK
    Structure 21:133-42. 2013
    ..The structure offers insight into how HBeAg may establish immune tolerance for HBcAg while evading its robust immunogenicity...
  54. pmc Remodeling of lipid vesicles into cylindrical micelles by α-synuclein in an extended α-helical conformation
    Naoko Mizuno
    Laboratory of Structural Biology, NIAMS, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 287:29301-11. 2012
    ..The observed geometrical relationship between αS and the micelle suggests that the wedging of its long α-helix into the outer leaflet of a membrane may cause curvature and an anisotropic partition of lipids, leading to tube formation...
  55. pmc In Sup35p filaments (the [PSI+] prion), the globular C-terminal domains are widely offset from the amyloid fibril backbone
    Ulrich Baxa
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Microbiol 79:523-32. 2011
    ....
  56. ncbi request reprint Mass analysis by scanning transmission electron microscopy and electron diffraction validate predictions of stacked beta-solenoid model of HET-s prion fibrils
    Anindito Sen
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 282:5545-50. 2007
    ..This is half the packing density of approximately 1 subunit per 0.47 nm previously obtained for fibrils of the yeast prion proteins, Ure2p and Sup35p, whence it follows that the respective amyloid architectures are basically different...
  57. ncbi request reprint Visualization of the binding of Hsc70 ATPase to clathrin baskets: implications for an uncoating mechanism
    J Bernard Heymann
    Laboratory of Structural Biology, NIAMS, National Institutes of Health, Bethesda, MD, USA
    J Biol Chem 280:7156-61. 2005
    ..Three Hsc70s remain bound to the dissociated triskelion, close to its trimerization hub...
  58. pmc Influenza virus pleiomorphy characterized by cryoelectron tomography
    Audray Harris
    Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:19123-7. 2006
    ....
  59. pmc Extensive proteolysis of head and inner body proteins by a morphogenetic protease in the giant Pseudomonas aeruginosa phage φKZ
    Julie A Thomas
    Biochemistry and Molecular Biology, University of Maryland Baltimore, MD 21201, USA
    Mol Microbiol 84:324-39. 2012
    ..Together the six abundant proteins sum to the estimated mass of the inner body (15-20 MDa). The identification of these proteins is important for future studies on the composition and function of the inner body...
  60. ncbi request reprint Signal transduction at a protein synapse
    Alasdair C Steven
    Laboratory of Structural Biology, NIAMS, Building 50, Room 1517, 50 South Drive MSC 8025, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 118:403-4. 2004
    ....
  61. ncbi request reprint The cryptophycin-tubulin ring structure indicates two points of curvature in the tubulin dimer
    Norman R Watts
    Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 41:12662-9. 2002
    ..We conclude that drug binding to one subunit (beta) results in two bends per dimer, affecting both subunits...
  62. pmc Bubblegrams reveal the inner body of bacteriophage φKZ
    Weimin Wu
    Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases NIAMS, National Institutes of Health, Bethesda, MD 20892, USA
    Science 335:182. 2012
    ..Here, we present a differential mapping procedure, based on the physical principle of protein's greater sensitivity to radiation damage compared with that of nucleic acid...
  63. ncbi request reprint Structure, function, and amyloidogenesis of fungal prions: filament polymorphism and prion variants
    Ulrich Baxa
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Adv Protein Chem 73:125-80. 2006
    ..We discuss a possible structural basis for this phenomenon...
  64. pmc Subdomain organization of the Acanthamoeba myosin IC tail from cryo-electron microscopy
    Takashi Ishikawa
    Laboratory of Structural Biology Research, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, and Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 101:12189-94. 2004
    ..The outer face of the BR is strategically exposed for membrane or vesicle binding...
  65. ncbi request reprint The axial channel of the 20S proteasome opens upon binding of the PA200 activator
    Joaquin Ortega
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bldg 50, Room 1517, 50 South Drive MSC 8025, Bethesda, MD 20892 8025, USA
    J Mol Biol 346:1221-7. 2005
    ..Thus, the activation mechanism of PA200 is expressed via its allosteric effects on the 20 S core particle, perhaps facilitating release of digestion products or the entrance of substrates...
  66. pmc Mechanism of inactivation on prion conversion of the Saccharomyces cerevisiae Ure2 protein
    Ulrich Baxa
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:5253-60. 2002
    ..These observations suggest that the amyloid content of these filaments is confined to their prion domain-containing backbones and imply that Ure2p is inactivated in [URE3] cells by a steric blocking mechanism...
  67. pmc Handedness of the herpes simplex virus capsid and procapsid
    Naiqian Cheng
    Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 76:7855-9. 2002
    ..We have determined the handedness of both the herpes simplex virus type 1 capsid and its precursor procapsid by a cryoelectron microscopic tilting method...
  68. pmc The morphogenic linker peptide of HBV capsid protein forms a mobile array on the interior surface
    Norman R Watts
    Protein Expression Laboratory, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Building 50, Room 1517, 50 South Drive MSC 8025, National Institutes of Health, Bethesda, MD 20892 8025, USA
    EMBO J 21:876-84. 2002
    ..We propose that linker peptides are attached to the capsid inner surface as hinged struts, forming a mobile array, an arrangement with implications for morphogenesis and the management of encapsidated nucleic acid...
  69. pmc Human immunodeficiency virus type 1 N-terminal capsid mutants containing cores with abnormally high levels of capsid protein and virtually no reverse transcriptase
    Shixing Tang
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    J Virol 77:12592-602. 2003
    ..Thus, taken together with the almost complete absence of RT in mutant cores, these findings can account for the failure of the three CA mutants to synthesize viral DNA following virus entry into cells...
  70. pmc Initial stages of V(D)J recombination: the organization of RAG1/2 and RSS DNA in the postcleavage complex
    Gabrielle J Grundy
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 35:217-27. 2009
    ..These first images of the V(D)J recombinase in its postcleavage state provide a framework for modeling RAG domains and their interactions with DNA...
  71. ncbi request reprint Cryo-electron microscopy of trichocyte (hard alpha-keratin) intermediate filaments reveals a low-density core
    Norman R Watts
    Laboratory of Structural Biology Research, National Institutes of Health, Bethesda, Maryland 20892 8025, USA
    J Struct Biol 137:109-18. 2002
    ..3 nm. These filaments appear to represent IF coated with associated proteins-perhaps, "high-sulfur" proteins-readied for incorporation into the filament-matrix biocomposite of the mature hair...
  72. pmc Recombinant HA1 produced in E. coli forms functional oligomers and generates strain-specific SRID potency antibodies for pandemic influenza vaccines
    Surender Khurana
    Division of Viral Products, Center for Biologics Evaluation and Research CBER, Food and Drug Administration, Bethesda, MD 20892, USA
    Vaccine 29:5657-65. 2011
    ..We conclude that bacterially expressed recombinant HA1 proteins can be produced rapidly and used to generate SRID potency reagents shortly after new influenza strains with pandemic potential are identified...
  73. ncbi request reprint The next ice age: cryo-electron tomography of intact cells
    Alasdair C Steven
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Trends Cell Biol 13:107-10. 2003
    ..Cryo-tomograms of Dictyostelium cells depict distinct populations of ribosomes, proteasomes and networks of actin filaments interconnected by branching or bundling, apparently controlled by strategically placed actin-associated proteins...
  74. ncbi request reprint Functional proteolytic complexes of the human mitochondrial ATP-dependent protease, hClpXP
    Sung Gyun Kang
    Laboratory of Cell Biology, NCI and Laboratory of Structural Biology, NIAMS, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:21095-102. 2002
    ..Our data also indicate that human ClpP has conserved sites required for interaction with eClpX but not eClpA, implying that the modes of interaction with ClpP may not be identical for ClpA and ClpX...
  75. ncbi request reprint Quasi-normal cornified cell envelopes in loricrin knockout mice imply the existence of a loricrin backup system
    Michal Jarnik
    Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892 8025, USA
    J Invest Dermatol 118:102-9. 2002
    ..These observations imply that, in the absence of loricrin, the mechanisms that govern cell envelope assembly function normally but employ different building-blocks...
  76. pmc mtCLIC/CLIC4, an organellular chloride channel protein, is increased by DNA damage and participates in the apoptotic response to p53
    Ester Fernandez-Salas
    Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 22:3610-20. 2002
    ....
  77. pmc Alternating translocation of protein substrates from both ends of ClpXP protease
    Joaquin Ortega
    Laboratory of Structural Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases and Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 21:4938-49. 2002
    ....
  78. ncbi request reprint The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs
    Aixia Zhang
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Mol Cell 9:11-22. 2002
    ..We also show that Hfq increases the OxyS RNA interaction with its target messages and propose that the enhancement of RNA-RNA pairing may be a general function of Hfq, Sm, and Sm-like proteins...
  79. ncbi request reprint Prion and non-prion amyloids of the HET-s prion forming domain
    Raimon Sabate
    Laboratoire de Génétique Moléculaire des Champignons, Institut de Biochimie et de Génétique Cellulaires, UMR 5095 CNRS Université de Bordeaux 2, 1 rue Camille St Saëns, 33077 Bordeaux Cedex, France
    J Mol Biol 370:768-83. 2007
    ..The altered properties of the amyloid assembled at pH 2 may arise from a perturbation in the subunit fold or fibrillar stacking...
  80. ncbi request reprint The turn of the screw: variations of the abundant beta-solenoid motif in passenger domains of Type V secretory proteins
    Andrey V Kajava
    Centre de Recherches de Biochimie Macromoleculaire, CNRS FRE 2593, 1919 Route de Mende, 34293 Montpellier, Cedex 5, France
    J Struct Biol 155:306-15. 2006
    ..To illustrate model-building from a coil template, we modeled a type-T4 beta-solenoid for TibA of Escherichia coli which is predicted to have two conserved polar residues, Thr and Gln, in interior positions...
  81. ncbi request reprint New HEAT-like repeat motifs in proteins regulating proteasome structure and function
    Andrey V Kajava
    Centre de Recherches de Biochimie Macromoleculaire, CNRS FRE 2593, 1919 Route de Mende, 34293 Montpellier, Cedex 5, France
    J Struct Biol 146:425-30. 2004
    ..Both PA200 and Ecm29 are composed almost entirely of such repeats, and therefore are likely to have alpha-helical solenoid structures. These observations lead us to speculate on how PA200 and Ecm29 may associate with proteasomes...
  82. ncbi request reprint Control of virus assembly: HK97 "Whiffleball" mutant capsids without pentons
    Yiyong Li
    Department of Biological Sciences, University of Pittsburgh, PA 15260, USA
    J Mol Biol 348:167-82. 2005
    ..The data therefore favor a model in which Prohead I assembly is regulated by conformational switching of the hexamer...
  83. ncbi request reprint Time-resolved molecular dynamics of bacteriophage HK97 capsid maturation interpreted by electron cryo-microscopy and X-ray crystallography
    William R Wikoff
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Struct Biol 153:300-6. 2006
    ....
  84. pmc A thermally induced phase transition in a viral capsid transforms the hexamers, leaving the pentamers unchanged
    James F Conway
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA
    J Struct Biol 158:224-32. 2007
    ..Pentamers, on the other hand, are more stably anchored and resist this thermal perturbation...
  85. pmc The structure of the poliovirus 135S cell entry intermediate at 10-angstrom resolution reveals the location of an externalized polypeptide that binds to membranes
    Doryen Bubeck
    Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA
    J Virol 79:7745-55. 2005
    ..These observations have forced a reexamination of current models for the role of the 135S particle in transmembrane pore formation and suggest testable alternatives...
  86. pmc High-resolution mass spectrometry of viral assemblies: molecular composition and stability of dimorphic hepatitis B virus capsids
    Charlotte Uetrecht
    Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA, Utrecht, The Netherlands
    Proc Natl Acad Sci U S A 105:9216-20. 2008
    ..4 GPa. This experimental approach, anchored on very precise and accurate mass measurements, appears to hold considerable potential for elucidating the assembly of viruses and other macromolecular particles...
  87. ncbi request reprint Prospects of electron cryotomography to visualize macromolecular complexes inside cellular compartments: implications of crowding
    Kay Grünewald
    Department of Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Biophys Chem 100:577-91. 2003
    ..An example of the latter-a 5-fold symmetric particle is-given. Second, electron cryotomography offers an incisive probe to examine crowding in different cellular compartments...
  88. ncbi request reprint A novel class of herpesvirus with bivalve hosts
    Andrew J Davison
    MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK
    J Gen Virol 86:41-53. 2005
    ..OsHV-1 thus represents a third major class of the herpesviruses...
  89. ncbi request reprint Control of crosslinking by quaternary structure changes during bacteriophage HK97 maturation
    Lu Gan
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Cell 14:559-69. 2004
    ..A structure derived from cryo-EM images reveals the free intermediate conformation of penton arms, supporting our model for coordinated movement of hexons and pentons on the capsid lattice...
  90. pmc Structural and functional similarities between the capsid proteins of bacteriophages T4 and HK97 point to a common ancestry
    Andrei Fokine
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, IN 47907, USA
    Proc Natl Acad Sci U S A 102:7163-8. 2005
    ..Structural data show that capsid proteins of most tailed phages, and some eukaryotic viruses, may have evolved from a common ancestor...
  91. ncbi request reprint Structural and enzymatic properties of the AAA protein Drg1p from Saccharomyces cerevisiae. Decoupling of intracellular function from ATPase activity and hexamerization
    Andriy Zakalskiy
    Institut fur Molekularbiologie, Biochemie und Mikrobiologie, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    J Biol Chem 277:26788-95. 2002
    ..These observations are discussed in terms of the inter-relationship of ATPase activity per se, oligomeric status, and intracellular function for AAA proteins...
  92. pmc Stability and shape of hepatitis B virus capsids in vacuo
    Charlotte Uetrecht
    Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands
    Angew Chem Int Ed Engl 47:6247-51. 2008
  93. ncbi request reprint Standard conformations of beta-arches in beta-solenoid proteins
    Jérôme Hennetin
    Centre de Recherches de Biochimie Macromoleculaire, CNRS FRE 2593, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    J Mol Biol 358:1094-105. 2006
    ....
  94. ncbi request reprint The parallel superpleated beta-structure as a model for amyloid fibrils of human amylin
    Andrey V Kajava
    Centre de Recherches de Biochimie Macromoleculaire, CNRS FRE 2593, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    J Mol Biol 348:247-52. 2005
    ..The model is consistent with current biophysical, biochemical and genetic data and, in particular, affords a plausible explanation for why rodent amylin does not form fibrils...
  95. ncbi request reprint Beta-rolls, beta-helices, and other beta-solenoid proteins
    Andrey V Kajava
    Centre de Recherches de Biochimie Macromoleculaire, CNRS FRE 2593, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    Adv Protein Chem 73:55-96. 2006
    ..Thus, identification of genes with putative beta-solenoid domains promises to be a fertile direction in the search for viable targets in the development of new antibiotics and vaccines...
  96. pmc A model for Ure2p prion filaments and other amyloids: the parallel superpleated beta-structure
    Andrey V Kajava
    Centre de Recherches de Biochimie Macromoleculaire, Centre National de la Recherche Scientifique FRE 2593, 1919 Route de Mende, 34293 Montpellier 5, France
    Proc Natl Acad Sci U S A 101:7885-90. 2004
    ..47 nm) and is readily adaptable to other amyloids, for instance the core of Sup35p filaments and glutamine expansions in huntingtin...