Stephan T Stern

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Role for nanomaterial-autophagy interaction in neurodegenerative disease
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Autophagy 4:1097-100. 2008
  2. pmc Prediction of nanoparticle prodrug metabolism by pharmacokinetic modeling of biliary excretion
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick Inc, Frederick National Lab for Cancer Research, Frederick, 21702, USA Electronic address
    J Control Release 172:558-67. 2013
  3. pmc Autophagy and lysosomal dysfunction as emerging mechanisms of nanomaterial toxicity
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    Part Fibre Toxicol 9:20. 2012
  4. ncbi request reprint Nanotechnology safety concerns revisited
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Toxicol Sci 101:4-21. 2008
  5. pmc Translational considerations for cancer nanomedicine
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick Inc, NCI Frederick, PO Box B, Frederick, MD 21702, USA
    J Control Release 146:164-74. 2010
  6. pmc Induction of autophagy in porcine kidney cells by quantum dots: a common cellular response to nanomaterials?
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Toxicol Sci 106:140-52. 2008
  7. pmc Synergistic combination therapy with nanoliposomal C6-ceramide and vinblastine is associated with autophagy dysfunction in hepatocarcinoma and colorectal cancer models
    Pavan P Adiseshaiah
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, United States
    Cancer Lett 337:254-65. 2013
  8. doi request reprint Monitoring lysosomal activity in nanoparticle-treated cells
    Barry W Neun
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:207-12. 2011
  9. pmc Common pitfalls in nanotechnology: lessons learned from NCI's Nanotechnology Characterization Laboratory
    Rachael M Crist
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA
    Integr Biol (Camb) 5:66-73. 2013
  10. pmc Fullerenol cytotoxicity in kidney cells is associated with cytoskeleton disruption, autophagic vacuole accumulation, and mitochondrial dysfunction
    Denise N Johnson-Lyles
    Nanotechnology Characterization Lab NCL, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    Toxicol Appl Pharmacol 248:249-58. 2010

Collaborators

Detail Information

Publications18

  1. ncbi request reprint Role for nanomaterial-autophagy interaction in neurodegenerative disease
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Autophagy 4:1097-100. 2008
    ..The interaction of nanomaterials with the autophagy pathway, and the potential negative consequences of resulting autophagy dysfunction, should be explored further...
  2. pmc Prediction of nanoparticle prodrug metabolism by pharmacokinetic modeling of biliary excretion
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick Inc, Frederick National Lab for Cancer Research, Frederick, 21702, USA Electronic address
    J Control Release 172:558-67. 2013
    ..Uses of such models may include interpretation of preclinical toxicology studies, selection of first in man dosing regimens, and PK/PD model development. ..
  3. pmc Autophagy and lysosomal dysfunction as emerging mechanisms of nanomaterial toxicity
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    Part Fibre Toxicol 9:20. 2012
    ..Indeed, there is ample evidence that biopersistent nanomaterials can cause autophagy and lysosomal dysfunctions resulting in toxicological consequences...
  4. ncbi request reprint Nanotechnology safety concerns revisited
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Toxicol Sci 101:4-21. 2008
    ..Until such time as the exposures, hazards, and environmental life cycle of nanomaterials have been more clearly defined, cautious development and implementation of nanotechnology is the most prudent course...
  5. pmc Translational considerations for cancer nanomedicine
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick Inc, NCI Frederick, PO Box B, Frederick, MD 21702, USA
    J Control Release 146:164-74. 2010
    ..Where possible, these recommendations are justified using the existing regulatory guidance literature...
  6. pmc Induction of autophagy in porcine kidney cells by quantum dots: a common cellular response to nanomaterials?
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Toxicol Sci 106:140-52. 2008
    ..These data suggest that QD cytotoxicity is dependent upon properties of the particle as a whole, and not exclusively the metal core materials...
  7. pmc Synergistic combination therapy with nanoliposomal C6-ceramide and vinblastine is associated with autophagy dysfunction in hepatocarcinoma and colorectal cancer models
    Pavan P Adiseshaiah
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, United States
    Cancer Lett 337:254-65. 2013
    ..In conclusion, in vitro and in vivo data support a synergistic antitumor activity of the nanoliposomal C6-ceramide and vinblastine combination, potentially mediated by an autophagy mechanism. ..
  8. doi request reprint Monitoring lysosomal activity in nanoparticle-treated cells
    Barry W Neun
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:207-12. 2011
    ..The lysotracker signal is normalized to the signal from a thiol-reactive dye which is proportional to the total number of viable cells...
  9. pmc Common pitfalls in nanotechnology: lessons learned from NCI's Nanotechnology Characterization Laboratory
    Rachael M Crist
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA
    Integr Biol (Camb) 5:66-73. 2013
    ..While not exhaustive, this article aims to share some of the most common pitfalls observed by the NCL as they relate to nanoparticle synthesis, purification, characterization and analysis...
  10. pmc Fullerenol cytotoxicity in kidney cells is associated with cytoskeleton disruption, autophagic vacuole accumulation, and mitochondrial dysfunction
    Denise N Johnson-Lyles
    Nanotechnology Characterization Lab NCL, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    Toxicol Appl Pharmacol 248:249-58. 2010
    ..As nanoparticle-induced cytoskeleton disruption, autophagic vacuole accumulation and mitochondrial dysfunction are commonly reported in the literature, the proposed mechanism may be relevant for a variety of nanomaterials...
  11. pmc Polymeric curcumin nanoparticle pharmacokinetics and metabolism in bile duct cannulated rats
    Peng Zou
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, United States
    Mol Pharm 10:1977-87. 2013
    ..Additionally, the remaining encapsulated curcumin fraction following burst release is available for tumor delivery via the enhanced permeation and retention effect commonly observed for nanoparticle formulations...
  12. doi request reprint Monitoring glutathione homeostasis in nanoparticle-treated hepatocytes
    Timothy M Potter
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:191-8. 2011
    ..The method presented in this chapter utilizes a colorimetric method for detection of reduced and oxidized glutathione...
  13. doi request reprint Assay to detect lipid peroxidation upon exposure to nanoparticles
    Timothy M Potter
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:181-9. 2011
    ..The results are then expressed as MDA equivalents, normalized to total cellular protein (determined by Bradford assay)...
  14. doi request reprint Autophagy monitoring assay: qualitative analysis of MAP LC3-I to II conversion by immunoblot
    Christopher B McLeland
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:199-206. 2011
    ....
  15. doi request reprint Detecting reactive oxygen species in primary hepatocytes treated with nanoparticles
    Banu Zolnik
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:173-9. 2011
    ....
  16. doi request reprint Evaluation of cytotoxicity of nanoparticulate materials in porcine kidney cells and human hepatocarcinoma cells
    Timothy M Potter
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:157-65. 2011
    ..In this standard, two separate metrics (MTT and LDH) provide complementary data, that can be used to identify interference...
  17. doi request reprint Rapid distribution of liposomal short-chain ceramide in vitro and in vivo
    Banu S Zolnik
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    Drug Metab Dispos 36:1709-15. 2008
    ..Our studies suggest that this nanoscale PEGylated drug delivery system for short-chain ceramide offers rapid tissue distribution without adverse effects for a neoplastic-selective, insoluble agent...
  18. doi request reprint Monitoring nanoparticle-treated hepatocarcinoma cells for apoptosis
    Timothy M Potter
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:167-72. 2011
    ..This chapter describes a method for monitoring nanoparticle treated human hepatocarcinoma cells (Hep G2) for apoptosis. The protocol utilizes a fluorescent method to determine the degree of caspase-3 activation...