Stephan T Stern

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi Role for nanomaterial-autophagy interaction in neurodegenerative disease
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Autophagy 4:1097-100. 2008
  2. ncbi Autophagy and lysosomal dysfunction as emerging mechanisms of nanomaterial toxicity
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    Part Fibre Toxicol 9:20. 2012
  3. ncbi Nanotechnology safety concerns revisited
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Toxicol Sci 101:4-21. 2008
  4. ncbi Induction of autophagy in porcine kidney cells by quantum dots: a common cellular response to nanomaterials?
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Toxicol Sci 106:140-52. 2008
  5. ncbi Translational considerations for cancer nanomedicine
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick Inc, NCI Frederick, PO Box B, Frederick, MD 21702, USA
    J Control Release 146:164-74. 2010
  6. ncbi Monitoring lysosomal activity in nanoparticle-treated cells
    Barry W Neun
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:207-12. 2011
  7. ncbi Fullerenol cytotoxicity in kidney cells is associated with cytoskeleton disruption, autophagic vacuole accumulation, and mitochondrial dysfunction
    Denise N Johnson-Lyles
    Nanotechnology Characterization Lab NCL, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    Toxicol Appl Pharmacol 248:249-58. 2010
  8. ncbi Monitoring glutathione homeostasis in nanoparticle-treated hepatocytes
    Timothy M Potter
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:191-8. 2011
  9. ncbi Common pitfalls in nanotechnology: lessons learned from NCI's Nanotechnology Characterization Laboratory
    Rachael M Crist
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA
    Integr Biol (Camb) 5:66-73. 2013
  10. ncbi Assay to detect lipid peroxidation upon exposure to nanoparticles
    Timothy M Potter
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:181-9. 2011

Collaborators

  • Scott E McNeil
  • Pavan P Adiseshaiah
  • Giulio F Paciotti
  • Timothy M Potter
  • Barry W Neun
  • Rachael M Crist
  • Christopher B McLeland
  • Banu Zolnik
  • Jeffrey D Clogston
  • Denise N Johnson-Lyles
  • Banu S Zolnik
  • Jennifer Hall Grossman
  • Anil K Patri
  • Marina A Dobrovolskaia
  • Jamie Rodriguez
  • Kimberly Peifley
  • Jeffrey Clogston
  • Stephen Lockett
  • Matthew Hansen
  • Mark Kester
  • Yasser Heakal
  • James M Kaiser

Detail Information

Publications15

  1. ncbi Role for nanomaterial-autophagy interaction in neurodegenerative disease
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Autophagy 4:1097-100. 2008
    ..The interaction of nanomaterials with the autophagy pathway, and the potential negative consequences of resulting autophagy dysfunction, should be explored further...
  2. ncbi Autophagy and lysosomal dysfunction as emerging mechanisms of nanomaterial toxicity
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    Part Fibre Toxicol 9:20. 2012
    ..Indeed, there is ample evidence that biopersistent nanomaterials can cause autophagy and lysosomal dysfunctions resulting in toxicological consequences...
  3. ncbi Nanotechnology safety concerns revisited
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Toxicol Sci 101:4-21. 2008
    ..Until such time as the exposures, hazards, and environmental life cycle of nanomaterials have been more clearly defined, cautious development and implementation of nanotechnology is the most prudent course...
  4. ncbi Induction of autophagy in porcine kidney cells by quantum dots: a common cellular response to nanomaterials?
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Toxicol Sci 106:140-52. 2008
    ..These data suggest that QD cytotoxicity is dependent upon properties of the particle as a whole, and not exclusively the metal core materials...
  5. ncbi Translational considerations for cancer nanomedicine
    Stephan T Stern
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick Inc, NCI Frederick, PO Box B, Frederick, MD 21702, USA
    J Control Release 146:164-74. 2010
    ..Where possible, these recommendations are justified using the existing regulatory guidance literature...
  6. ncbi Monitoring lysosomal activity in nanoparticle-treated cells
    Barry W Neun
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:207-12. 2011
    ..The lysotracker signal is normalized to the signal from a thiol-reactive dye which is proportional to the total number of viable cells...
  7. ncbi Fullerenol cytotoxicity in kidney cells is associated with cytoskeleton disruption, autophagic vacuole accumulation, and mitochondrial dysfunction
    Denise N Johnson-Lyles
    Nanotechnology Characterization Lab NCL, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    Toxicol Appl Pharmacol 248:249-58. 2010
    ..As nanoparticle-induced cytoskeleton disruption, autophagic vacuole accumulation and mitochondrial dysfunction are commonly reported in the literature, the proposed mechanism may be relevant for a variety of nanomaterials...
  8. ncbi Monitoring glutathione homeostasis in nanoparticle-treated hepatocytes
    Timothy M Potter
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:191-8. 2011
    ..The method presented in this chapter utilizes a colorimetric method for detection of reduced and oxidized glutathione...
  9. ncbi Common pitfalls in nanotechnology: lessons learned from NCI's Nanotechnology Characterization Laboratory
    Rachael M Crist
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA
    Integr Biol (Camb) 5:66-73. 2013
    ..While not exhaustive, this article aims to share some of the most common pitfalls observed by the NCL as they relate to nanoparticle synthesis, purification, characterization and analysis...
  10. ncbi Assay to detect lipid peroxidation upon exposure to nanoparticles
    Timothy M Potter
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:181-9. 2011
    ..The results are then expressed as MDA equivalents, normalized to total cellular protein (determined by Bradford assay)...
  11. ncbi Autophagy monitoring assay: qualitative analysis of MAP LC3-I to II conversion by immunoblot
    Christopher B McLeland
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:199-206. 2011
    ....
  12. ncbi Detecting reactive oxygen species in primary hepatocytes treated with nanoparticles
    Banu Zolnik
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:173-9. 2011
    ....
  13. ncbi Evaluation of cytotoxicity of nanoparticulate materials in porcine kidney cells and human hepatocarcinoma cells
    Timothy M Potter
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:157-65. 2011
    ..In this standard, two separate metrics (MTT and LDH) provide complementary data, that can be used to identify interference...
  14. ncbi Rapid distribution of liposomal short-chain ceramide in vitro and in vivo
    Banu S Zolnik
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    Drug Metab Dispos 36:1709-15. 2008
    ..Our studies suggest that this nanoscale PEGylated drug delivery system for short-chain ceramide offers rapid tissue distribution without adverse effects for a neoplastic-selective, insoluble agent...
  15. ncbi Monitoring nanoparticle-treated hepatocarcinoma cells for apoptosis
    Timothy M Potter
    Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD, USA
    Methods Mol Biol 697:167-72. 2011
    ..This chapter describes a method for monitoring nanoparticle treated human hepatocarcinoma cells (Hep G2) for apoptosis. The protocol utilizes a fluorescent method to determine the degree of caspase-3 activation...