Joseph Shiloach

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Glucose depletion activates mmu-miR-466h-5p expression through oxidative stress and inhibition of histone deacetylation
    Aliaksandr Druz
    Biotechnology Core Laboratory National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Health Bldg 14A Bethesda, MD 20892, USA
    Nucleic Acids Res 40:7291-302. 2012
  2. ncbi request reprint Production of recombinant protein by a novel oxygen-induced system in Escherichia coli
    Antonino Baez
    Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Microb Cell Fact 13:50. 2014
  3. doi request reprint Analyzing metabolic variations in different bacterial strains, historical perspectives and current trends--example E. coli
    Joseph Shiloach
    Biotechnology Laboratory, NIDDK, NIH Bethesda, MD 20892, USA
    Curr Opin Biotechnol 21:21-6. 2010
  4. ncbi request reprint Growing E. coli to high cell density--a historical perspective on method development
    Joseph Shiloach
    Biotechnology Unit, Bldg 14A Rm 173, NIDDK, NIH Bethesda, MD 20892 5522, USA
    Biotechnol Adv 23:345-57. 2005
  5. pmc A novel microRNA mmu-miR-466h affects apoptosis regulation in mammalian cells
    Aliaksandr Druz
    Biotechnology Core Laboratory National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 9000 Rockville Pike Bldg 14A Rm 176, Bethesda, Maryland 20892, USA
    Biotechnol Bioeng 108:1651-61. 2011
  6. pmc Structural basis of toll-like receptor 3 signaling with double-stranded RNA
    Lin Liu
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Science 320:379-81. 2008
  7. doi request reprint Acetate accumulation through alternative metabolic pathways in ackA (-) pta (-) poxB (-) triple mutant in E. coli B (BL21)
    Je Nie Phue
    Biotechnology Core Laboratory, NIDDK NIH Bethesda, Bldg 14A Room 173, Bethesda, MD 20892, USA
    Biotechnol Lett 32:1897-903. 2010
  8. ncbi request reprint Impact of dissolved oxygen concentration on acetate accumulation and physiology of E. coli BL21, evaluating transcription levels of key genes at different dissolved oxygen conditions
    Je Nie Phue
    Biotechnology Unit, NIDDK, NIH, Bldg 14A Room 173, Bethesda, MD 20892, USA
    Metab Eng 7:353-63. 2005
  9. pmc Escherichia coli avoids high dissolved oxygen stress by activation of SoxRS and manganese-superoxide dismutase
    Antonino Baez
    Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Microb Cell Fact 12:23. 2013
  10. pmc Bacillus anthracis cell wall peptidoglycan but not lethal or edema toxins produces changes consistent with disseminated intravascular coagulation in a rat model
    Ping Qiu
    Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland, USA
    J Infect Dis 208:978-89. 2013

Collaborators

Detail Information

Publications52

  1. pmc Glucose depletion activates mmu-miR-466h-5p expression through oxidative stress and inhibition of histone deacetylation
    Aliaksandr Druz
    Biotechnology Core Laboratory National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Health Bldg 14A Bethesda, MD 20892, USA
    Nucleic Acids Res 40:7291-302. 2012
    ..Improved characterization of the molecular events that lead to the activation of miR-466h-5p may provide a better understanding of the relation between cellular environment and miRNA activation...
  2. ncbi request reprint Production of recombinant protein by a novel oxygen-induced system in Escherichia coli
    Antonino Baez
    Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Microb Cell Fact 13:50. 2014
    ..It also does not affect the composition of the growth medium simplifying the recovery and purification processes...
  3. doi request reprint Analyzing metabolic variations in different bacterial strains, historical perspectives and current trends--example E. coli
    Joseph Shiloach
    Biotechnology Laboratory, NIDDK, NIH Bethesda, MD 20892, USA
    Curr Opin Biotechnol 21:21-6. 2010
    ..The review focuses on the different methodologies and their use, as well as historical overview of the evaluation of the differences between Escherichia coli K and E. coli B...
  4. ncbi request reprint Growing E. coli to high cell density--a historical perspective on method development
    Joseph Shiloach
    Biotechnology Unit, Bldg 14A Rm 173, NIDDK, NIH Bethesda, MD 20892 5522, USA
    Biotechnol Adv 23:345-57. 2005
    ..Additional research on the effects of heterologous protein biosynthesis on signal transduction, proteolysis and post transcription events in E. coli may improve its productivity...
  5. pmc A novel microRNA mmu-miR-466h affects apoptosis regulation in mammalian cells
    Aliaksandr Druz
    Biotechnology Core Laboratory National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 9000 Rockville Pike Bldg 14A Rm 176, Bethesda, Maryland 20892, USA
    Biotechnol Bioeng 108:1651-61. 2011
    ..This suggests the pro-apoptotic role of mmu-miR-466h and its capability to modulate the apoptotic pathway in mammalian cells...
  6. pmc Structural basis of toll-like receptor 3 signaling with double-stranded RNA
    Lin Liu
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Science 320:379-81. 2008
    ..This juxtaposition could mediate downstream signaling by dimerizing the cytoplasmic Toll interleukin-1 receptor (TIR) domains. The overall shape of the TLR3-ECD does not change upon binding to dsRNA...
  7. doi request reprint Acetate accumulation through alternative metabolic pathways in ackA (-) pta (-) poxB (-) triple mutant in E. coli B (BL21)
    Je Nie Phue
    Biotechnology Core Laboratory, NIDDK NIH Bethesda, Bldg 14A Room 173, Bethesda, MD 20892, USA
    Biotechnol Lett 32:1897-903. 2010
    ..It is likely that there are alternative acetate production pathways...
  8. ncbi request reprint Impact of dissolved oxygen concentration on acetate accumulation and physiology of E. coli BL21, evaluating transcription levels of key genes at different dissolved oxygen conditions
    Je Nie Phue
    Biotechnology Unit, NIDDK, NIH, Bldg 14A Room 173, Bethesda, MD 20892, USA
    Metab Eng 7:353-63. 2005
    ..Oxygen limitation seems to repress the constitutive expression of the glyoxylate shunt and gluconeognesis. In this work, the concept of transition state is proposed to describe the bacterial response to the lower DO concentration...
  9. pmc Escherichia coli avoids high dissolved oxygen stress by activation of SoxRS and manganese-superoxide dismutase
    Antonino Baez
    Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Microb Cell Fact 12:23. 2013
    ..coli, it was important to investigate the effect of high dissolved oxygen concentrations on the physiology and growth of E. coli and the way it responds to oxidative stress...
  10. pmc Bacillus anthracis cell wall peptidoglycan but not lethal or edema toxins produces changes consistent with disseminated intravascular coagulation in a rat model
    Ping Qiu
    Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, Maryland, USA
    J Infect Dis 208:978-89. 2013
    ..Although lethal toxin (LT) and edema toxin (ET) could contribute, B. anthracis cell wall peptidoglycan (PGN), not the toxins, stimulates inflammatory responses associated with DIC...
  11. pmc Evaluating microarrays using a semiparametric approach: application to the central carbon metabolism of Escherichia coli BL21 and JM109
    Je Nie Phue
    Biotechnology Unit, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 14A, Room 170, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Genomics 89:300-5. 2007
    ..These results further characterized differences between the two E. coli strains and illustrated the potency of the semiparametric algorithm...
  12. ncbi request reprint Production of recombinant proteins by vaccinia virus in a microcarrier based mammalian cell perfusion bioreactor
    Nicole A Bleckwenn
    Biotechnology Unit, NIDDK, National Institutes of Health, DHHS, Bldg 14A Rm 173, MSC 5522, 9000 Rockville Pike, Bethesda, Maryland, USA
    Biotechnol Bioeng 90:663-74. 2005
    ..Also, biological activity was assayed, resulting in an ID50 of 3.1 microg/mL, which is comparable to previous reports...
  13. ncbi request reprint Preparation, characterization, and immunogenicity in mice of a recombinant influenza H5 hemagglutinin vaccine against the avian H5N1 A/Vietnam/1203/2004 influenza virus
    Zuzana Biesova
    Program in Developmental and Molecular Immunity, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2423, United States
    Vaccine 27:6234-8. 2009
    ..Using this method, rHA vaccine can be produced in 3-4 weeks and when formulated with alum, induces HA antibody levels in young outbred mice consistent with the FDA guidelines for vaccines against epidemic and pandemic influenza...
  14. doi request reprint Large-scale cell culture
    Nicole A Bleckwenn
    NIDDK, National Institutes of Health, Bethesda, Maryland, USA
    Curr Protoc Immunol . 2004
    ..This unit focuses on the methods used to cultivate large amounts of cells for the purpose of obtaining an endogenous or recombinant product...
  15. pmc Toward a new vaccine for pertussis
    John B Robbins
    National Institute of Child Health and Human Development and National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 111:3213-6. 2014
    ..Such conjugates are easy to prepare, stable, and should add both to the level and duration of immunity induced by current vaccine-induced pertussis antibodies and reduce the circulation of B. pertussis. ..
  16. pmc Enhancement of cell proliferation in various mammalian cell lines by gene insertion of a cyclin-dependent kinase homolog
    Pratik Jaluria
    National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Biotechnology Unit, Building 14A, Room 170, Bethesda, MD 20892, USA
    BMC Biotechnol 7:71. 2007
    ....
  17. pmc The role of Cra in regulating acetate excretion and osmotic tolerance in E. coli K-12 and E. coli B at high density growth
    Young Jin Son
    Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases NIH, Bethesda, MD 20892, USA
    Microb Cell Fact 10:52. 2011
    ..coli B (BL21) and E. coli K-12 (JM109) were prepared and their growth behaviour and gene expression at high glucose were evaluated using microarray and real-time PCR...
  18. pmc Reducing acetate excretion from E. coli K-12 by over-expressing the small RNA SgrS
    Alejandro Negrete
    Biotechnology Core Laboratory, NIDDK, NIH, Bethesda, MD, USA
    N Biotechnol 30:269-73. 2013
    ..coli B. This observation opens a new approach towards controlling bacterial metabolism through the use of noncoding RNA...
  19. ncbi request reprint Glucose metabolism at high density growth of E. coli B and E. coli K: differences in metabolic pathways are responsible for efficient glucose utilization in E. coli B as determined by microarrays and Northern blot analyses
    Je Nie Phue
    Biotechnology Unit, NIH NIDDK, Bethesda, Maryland 20892 2715, USA
    Biotechnol Bioeng 90:805-20. 2005
    ..The gluconeogenesis pathway (fbp, pckA, and ppsA) which leads to glycogen accumulation is constitutively active in E. coli BL21 regardless of glucose feeding strategy...
  20. ncbi request reprint Production of recombinant protein using the HeLa S3-vaccinia virus expression system: bioreactor perfusion and effects of post-infection temperature
    Nicole A Bleckwenn
    Biotechnology Unit, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    Biosci Biotechnol Biochem 69:1065-72. 2005
    ..Scaling up the process to a 1.5-liter bioreactor with hollow fiber perfusion led to an overall production level of 9.9 microg EGFP/10(6) infected cells, or 27 mg EGFP per liter...
  21. ncbi request reprint The molecular structure of the TLR3 extracellular domain
    Jessica K Bell
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases NIH, 10 Center Drive, Bethesda, MD 20892, USA
    J Endotoxin Res 12:375-8. 2006
    ..Mutational and co-crystallization studies are currently underway to determine how TLR3 binds its ligand at the molecular level...
  22. pmc Stable inhibition of mmu-miR-466h-5p improves apoptosis resistance and protein production in CHO cells
    Aliaksandr Druz
    Biotechnology Core Laboratory NIDDK, NIH, Building 14A, Bethesda, MD 20892, USA
    Metab Eng 16:87-94. 2013
    ..The above results demonstrate the potential of this novel approach to create more productive cell lines through stable manipulation of specific miRNA expression...
  23. ncbi request reprint Screen-less expanded bed column: new approach for the recovery and purification of a malaria transmission blocking vaccine candidate from Pichia pastoris
    Loc Trinh
    Biotechnology Unit, NIDDK, NIH Bldg 14A, Room 173, Bethesda, MD 20892, USA
    Biotechnol Lett 28:951-8. 2006
    ..The screen-less design did not affect the binding capacity, purification level or process yield and, therefore, shorten the process. Purified Pfs25H of 6.4 g were recovered from 37 l of Pichia pastoris culture in one step...
  24. doi request reprint Production, purification, and characterization of human alpha1 proteinase inhibitor from Aspergillus niger
    Liat Chill
    Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 14A Room 170, 9000 Rockville Pike, Bethesda, Maryland 20892, USA
    Biotechnol Bioeng 102:828-44. 2009
    ..The Galactofuranose-containing high-mannnose type N-glycans are typical structures, which recently have been found as part of several glycoproteins produced by Aspergillus niger...
  25. ncbi request reprint Vaccinia virus-based expression of gp120 and EGFP: survey of mammalian host cell lines
    Nicole A Bleckwenn
    Biotechnology Unit, NIDDK, National Institutes of Health, DHHS, Bldg 14A Rm 173, 9000 Rockville Pike, Bethesda, Maryland 20892, USA
    Biotechnol Prog 21:186-91. 2005
    ..On the basis of these results and overall process considerations, 293 cells are recommended for further production process optimization in a serum-free suspension system...
  26. ncbi request reprint Large-scale expression and purification of a G-protein-coupled receptor for structure determination -- an overview
    Reinhard Grisshammer
    Laboratory of Molecular Biology of the National Institute of Diabetes and Digestive and Kidney Diseases, Department of Health and Human Services, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Struct Funct Genomics 6:159-63. 2005
    ..Functional expression in Escherichia coli and an automated two-column purification routine allow ongoing crystallization experiments and studies on receptor-bound ligands...
  27. ncbi request reprint Evaluation of production parameters with the vaccinia virus expression system using microcarrier attached HeLa cells
    Nicole A Bleckwenn
    Biotechnology Unit, NIDDK, National Institutes of Health, DHHS, 9000 Rockville Pike, Bethesda, Maryland 20892, USA
    Biotechnol Prog 21:554-61. 2005
    ..The level of production achieved with this system reached approximately 17 microg EGFP/10(6) infected cells...
  28. ncbi request reprint Egr1 and Gas6 facilitate the adaptation of HEK-293 cells to serum-free media by conferring enhanced viability and higher growth rates
    Pratik Jaluria
    National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 9000 Rockville Pike, Biotechnology Unit, Building 14A, Room 173, Bethesda, Maryland 20892, USA
    Biotechnol Bioeng 99:1443-52. 2008
    ..These results indicate that enhanced expression of egr1 or gas6 facilitate adaptation to SFM by improving growth and viability...
  29. pmc Long-lasting and transmission-blocking activity of antibodies to Plasmodium falciparum elicited in mice by protein conjugates of Pfs25
    Joanna Kubler-Kielb
    Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health, 31 Center Drive, MSC 2423, Bethesda, MD 20892 2520, USA
    Proc Natl Acad Sci U S A 104:293-8. 2007
    ..The observed transmission-blocking activity of immune sera correlated with antibody levels measured by ELISA...
  30. pmc Large-scale screening identifies a novel microRNA, miR-15a-3p, which induces apoptosis in human cancer cell lines
    Aliaksandr Druz
    Biotechnology Core Laboratory, The National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    RNA Biol 10:287-300. 2013
    ....
  31. pmc Synthesis, characterization, and immunogenicity in mice of Shigella sonnei O-specific oligosaccharide-core-protein conjugates
    John B Robbins
    National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:7974-8. 2009
    ..IgG antibody levels induced in young outbred mice by the S. sonnei O-SPC conjugates were significantly higher then those elicited by the O-SP conjugates. Accordingly, we propose to evaluate clinically these conjugates...
  32. doi request reprint Modified Escherichia coli B (BL21), a superior producer of plasmid DNA compared with Escherichia coli K (DH5alpha)
    Je Nie Phue
    Biotechnology Core Laboratory, NIDDK NIH Bethesda, Bldg 14A Room 173, Maryland 20892, USA
    Biotechnol Bioeng 101:831-6. 2008
    ..The produced plasmid, which was constructed for HIV clinical study, was found to have identical properties to the plasmid currently produced by E. coli DH5alpha...
  33. pmc Phase 1 study of a recombinant mutant protective antigen of Bacillus anthracis
    Joseph A Bellanti
    Program in Developmental and Molecular Immunity, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
    Clin Vaccine Immunol 19:140-5. 2012
    ..There were too few AVA recipients for statistical comparisons, but their antibody levels followed those of rPA. Anti-rPA measured by ELISA correlated with TNA titers (r = 0.97). These data support studying alum-adsorbed rPA in children...
  34. pmc Glucose uptake regulation in E. coli by the small RNA SgrS: comparative analysis of E. coli K-12 (JM109 and MG1655) and E. coli B (BL21)
    Alejandro Negrete
    Biotechnology Core Laboratory, NIDDK, NIH, Bethesda, MD, USA
    Microb Cell Fact 9:75. 2010
    ..The increased level of sRNA SgrS reduces the level of the ptsG mRNA and consequently lowers the level of the glucose transporter IICBGlc. The suggested trigger for this action is the accumulation of the corresponding phospho-sugars...
  35. ncbi request reprint Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from Pichia pastoris
    L B Trinh
    Biotechnology Unit, National Institutes of Health, NIDDK Building 6, Room B1 33, Bethesda, Maryland 20892 2715, USA
    Biotechnol Bioeng 82:438-44. 2003
    ....
  36. pmc Dimerization of the class A G protein-coupled neurotensin receptor NTS1 alters G protein interaction
    Jim F White
    Membrane Protein Structure and Function Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 104:12199-204. 2007
    ..NTS1 monomers activate G alpha q beta(1)gamma(2), whereas receptor dimers catalyze nucleotide exchange with lower affinity. Our results demonstrate that NTS1 dimerization per se is not a prerequisite for G protein activation...
  37. pmc Transient and stable expression of the neurotensin receptor NTS1: a comparison of the baculovirus-insect cell and the T-REx-293 expression systems
    Su Xiao
    Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland, United States of America
    PLoS ONE 8:e63679. 2013
    ..This work demonstrates two approaches for preparing milligram quantities of purified NTS1 suitable for structural studies and provides useful input to users in choosing and optimizing an appropriate expression host for other GPCRs...
  38. pmc Structure of the agonist-bound neurotensin receptor
    Jim F White
    Membrane Protein Structure Function Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Department of Health and Human Services, Rockville, Maryland 20852, USA
    Nature 490:508-13. 2012
    ....
  39. doi request reprint Production and antigenic properties of influenza virus from suspension MDCK-siat7e cells in a bench-scale bioreactor
    Chia Chu
    National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Vaccine 28:7193-201. 2010
    ..As a proof-of-concept this work demonstrates that by using a proper strategy, high yields of biologically active hemagglutinin can be produced from scalable cultures of suspension MDCK-siat7e cells...
  40. pmc The molecular structure of the Toll-like receptor 3 ligand-binding domain
    Jessica K Bell
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:10976-80. 2005
    ..The TLR3-ECD structure indicates how LRR loops can establish distinct pathogen recognition receptors...
  41. ncbi request reprint Transcription levels of key metabolic genes are the cause for different glucose utilization pathways in E. coli B (BL21) and E. coli K (JM109)
    Je Nie Phue
    Biotechnology Unit, NIH, NIDDK, Building 14A Rm 173, Bethesda, MD 20892, USA
    J Biotechnol 109:21-30. 2004
    ..The transcript of acs is higher in E. coli BL21 and lower in the E. coli JM109, while the reverse is true for poxB transcription...
  42. ncbi request reprint Mass spectrometry analysis of recombinant human ZP3 expressed in glycosylation-deficient CHO cells
    Ming Zhao
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 43:12090-104. 2004
    ..Taken together, these data indicate that human and mouse ZP3 proteins are quite similar, and alternative explanations of taxon-specific sperm binding warrant exploration...
  43. pmc Bacillus anthracis cell wall produces injurious inflammation but paradoxically decreases the lethality of anthrax lethal toxin in a rat model
    Xizhong Cui
    Critical Care Medicine Department, Clinical Center, National Institutes of Health, Building 10, Room 2C148, Bethesda, MD 20892, USA
    Intensive Care Med 36:148-56. 2010
    ..We therefore investigated these effects in rats and, for comparison, those of known inflammatory stimulants, Staphylococcus aureus cell wall or lipopolysaccharide (LPS)...
  44. ncbi request reprint Development of a novel vaccinia-neutralization assay based on reporter-gene expression
    Jody Manischewitz
    Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drugs Administration, Bethesda, Maryland 20892, USA
    J Infect Dis 188:440-8. 2003
    ..The new assay will serve as an important tool both for preclinical and clinical trials of new smallpox vaccines and for evaluation of therapeutic agents to treat vaccine-associated adverse reactions...
  45. pmc Conjugates of group A and W135 capsular polysaccharides of neisseria meningitidis bound to recombinant Staphylococcus aureus enterotoxin C1: preparation, physicochemical characterization, and immunological properties in mice
    Zhigang Jin
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Infect Immun 73:7887-93. 2005
    ..A bivalent formulation of GAMP-rSEC and W135P-rSEC elicited IgG anti-CP at comparable levels to those induced by the conjugates administered separately...
  46. ncbi request reprint Exploring vaccinia virus as a tool for large-scale recombinant protein expression
    Nicole A Bleckwenn
    Biotechnology Unit, LCDB, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biotechnol Prog 19:130-6. 2003
    ..2 microg EGFP/(million cells at the time of infection), demonstrating the ability of this system to successfully express recombinant proteins at larger scale...
  47. ncbi request reprint Endostatin capture from Pichia pastoris culture in a fluidized bed. From on-chip process optimization to application
    Joseph Shiloach
    Biotechnology Unit, NIDDK, Bethesda, MD, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 790:327-36. 2003
    ....
  48. ncbi request reprint The rise in pertussis cases urges replacement of chemically-inactivated with genetically-inactivated toxoid for DTP
    John B Robbins
    National Institute of Child Health and Human Development, National Institutes of Health, 31 Center Drive, Bethesda, MD 20892 2423, USA
    Vaccine 25:2811-6. 2007
    ..Manufacturers have declined to produce the genetically-inactivated PTx because of the expense required to change to the improved vaccine and not because of scientific issues...
  49. doi request reprint Use of hollow fiber tangential flow filtration for the recovery and concentration of HIV virus-like particles produced in insect cells
    Alejandro Negrete
    Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, United States Electronic address
    J Virol Methods 195:240-6. 2014
    ..These downstream processing conditions are extremely valuable for the further large-scale purification process development for HIV gag-VLPs and other particulate bioproducts...
  50. ncbi request reprint Automated large-scale purification of a G protein-coupled receptor for neurotensin
    Jim F White
    National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892 8030, USA
    FEBS Lett 564:289-93. 2004
    ..The presented expression and purification procedures are simple and robust, and provide the basis for crystallization experiments of receptors on a routine basis...
  51. pmc Dequalinium, a new inhibitor of Mycobacterium tuberculosis mycothiol ligase identified by high-throughput screening
    Maria Teresa Gutierrez-Lugo
    Laboratory of Bioorganic Chemistry, Biotechnology Unit, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20817, USA
    J Biomol Screen 14:643-52. 2009
    ..2 and 0.3 microg/mL, respectively. The screening protocol described is robust and has enabled the identification of new MshC inhibitors...
  52. ncbi request reprint MKR mice are resistant to the metabolic actions of both insulin and adiponectin: discordance between insulin resistance and adiponectin responsiveness
    Chul Hee Kim
    Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Am J Physiol Endocrinol Metab 291:E298-305. 2006
    ..MKR mice may be a useful model for dissecting relationships between insulin resistance and adiponectin action in regulation of glucose homeostasis...