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Genomes and Genes | Joseph ShiloachSummaryAffiliation: National Institutes of Health Country: USA Publications
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Publications
Escherichia coli avoids high dissolved oxygen stress by activation of SoxRS and manganese-superoxide dismutaseAntonino Baez
Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Microb Cell Fact 12:23. 2013..coli, it was important to investigate the effect of high dissolved oxygen concentrations on the physiology and growth of E. coli and the way it responds to oxidative stress...
Growing E. coli to high cell density--a historical perspective on method developmentJoseph Shiloach
Biotechnology Unit, Bldg 14A Rm 173, NIDDK, NIH Bethesda, MD 20892 5522, USA
Biotechnol Adv 23:345-57. 2005..Additional research on the effects of heterologous protein biosynthesis on signal transduction, proteolysis and post transcription events in E. coli may improve its productivity...
Analyzing metabolic variations in different bacterial strains, historical perspectives and current trends--example E. coliJoseph Shiloach
Biotechnology Laboratory, NIDDK, NIH Bethesda, MD 20892, USA
Curr Opin Biotechnol 21:21-6. 2010..The review focuses on the different methodologies and their use, as well as historical overview of the evaluation of the differences between Escherichia coli K and E. coli B...- Acetate accumulation through alternative metabolic pathways in ackA (-) pta (-) poxB (-) triple mutant in E. coli B (BL21)Je Nie Phue
Biotechnology Core Laboratory, NIDDK NIH Bethesda, Bldg 14A Room 173, Bethesda, MD 20892, USA
Biotechnol Lett 32:1897-903. 2010..It is likely that there are alternative acetate production pathways... - Structural basis of toll-like receptor 3 signaling with double-stranded RNALin Liu
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Science 320:379-81. 2008..This juxtaposition could mediate downstream signaling by dimerizing the cytoplasmic Toll interleukin-1 receptor (TIR) domains. The overall shape of the TLR3-ECD does not change upon binding to dsRNA... - Production of recombinant proteins by vaccinia virus in a microcarrier based mammalian cell perfusion bioreactorNicole A Bleckwenn
Biotechnology Unit, NIDDK, National Institutes of Health, DHHS, Bldg. 14A Rm. 173, MSC 5522, 9000 Rockville Pike, Bethesda, Maryland, USA
Biotechnol Bioeng 90:663-74. 2005..Also, biological activity was assayed, resulting in an ID50 of 3.1 microg/mL, which is comparable to previous reports... - Evaluating microarrays using a semiparametric approach: application to the central carbon metabolism of Escherichia coli BL21 and JM109Je Nie Phue
Biotechnology Unit, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 14A, Room 170, 9000 Rockville Pike, Bethesda, MD 20892, USA
Genomics 89:300-5. 2007..These results further characterized differences between the two E. coli strains and illustrated the potency of the semiparametric algorithm... - Impact of dissolved oxygen concentration on acetate accumulation and physiology of E. coli BL21, evaluating transcription levels of key genes at different dissolved oxygen conditionsJe-Nie Phue
Biotechnology Unit, NIDDK, NIH, Bldg 14A Room 173, Bethesda, MD 20892, USA
Metab Eng 7:353-63. 2005..Oxygen limitation seems to repress the constitutive expression of the glyoxylate shunt and gluconeognesis. In this work, the concept of transition state is proposed to describe the bacterial response to the lower DO concentration... - Preparation, characterization, and immunogenicity in mice of a recombinant influenza H5 hemagglutinin vaccine against the avian H5N1 A/Vietnam/1203/2004 influenza virusZuzana Biesova
Program in Developmental and Molecular Immunity, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2423, United States
Vaccine 27:6234-8. 2009..Using this method, rHA vaccine can be produced in 3-4 weeks and when formulated with alum, induces HA antibody levels in young outbred mice consistent with the FDA guidelines for vaccines against epidemic and pandemic influenza... - Large-scale cell cultureNicole A Bleckwenn
NIDDK, National Institutes of Health, Bethesda, Maryland, USA
Curr Protoc Immunol . 2004..This unit focuses on the methods used to cultivate large amounts of cells for the purpose of obtaining an endogenous or recombinant product... - The role of Cra in regulating acetate excretion and osmotic tolerance in E. coli K-12 and E. coli B at high density growthYoung Jin Son
Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases NIH, Bethesda, MD 20892, USA
Microb Cell Fact 10:52. 2011..coli B (BL21) and E. coli K-12 (JM109) were prepared and their growth behaviour and gene expression at high glucose were evaluated using microarray and real-time PCR... - Glucose metabolism at high density growth of E. coli B and E. coli K: differences in metabolic pathways are responsible for efficient glucose utilization in E. coli B as determined by microarrays and Northern blot analysesJe Nie Phue
Biotechnology Unit, NIH NIDDK, Bethesda, Maryland 20892 2715, USA
Biotechnol Bioeng 90:805-20. 2005..The gluconeogenesis pathway (fbp, pckA, and ppsA) which leads to glycogen accumulation is constitutively active in E. coli BL21 regardless of glucose feeding strategy... - Production of recombinant protein using the HeLa S3-vaccinia virus expression system: bioreactor perfusion and effects of post-infection temperatureNicole A Bleckwenn
Biotechnology Unit, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
Biosci Biotechnol Biochem 69:1065-72. 2005..Scaling up the process to a 1.5-liter bioreactor with hollow fiber perfusion led to an overall production level of 9.9 microg EGFP/10(6) infected cells, or 27 mg EGFP per liter... - The molecular structure of the TLR3 extracellular domainJessica K Bell
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases NIH, 10 Center Drive, Bethesda, MD 20892, USA
J Endotoxin Res 12:375-8. 2006..Mutational and co-crystallization studies are currently underway to determine how TLR3 binds its ligand at the molecular level... - Vaccinia virus-based expression of gp120 and EGFP: survey of mammalian host cell linesNicole A Bleckwenn
Biotechnology Unit, NIDDK, National Institutes of Health, DHHS, Bldg 14A Rm 173, 9000 Rockville Pike, Bethesda, Maryland 20892, USA
Biotechnol Prog 21:186-91. 2005..On the basis of these results and overall process considerations, 293 cells are recommended for further production process optimization in a serum-free suspension system... - Enhancement of cell proliferation in various mammalian cell lines by gene insertion of a cyclin-dependent kinase homologPratik Jaluria
National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Biotechnology Unit, Building 14A, Room 170, Bethesda, MD 20892, USA
BMC Biotechnol 7:71. 2007.... - Large-scale expression and purification of a G-protein-coupled receptor for structure determination -- an overviewReinhard Grisshammer
Laboratory of Molecular Biology of the National Institute of Diabetes and Digestive and Kidney Diseases, Department of Health and Human Services, National Institutes of Health, Bethesda, Maryland 20892, USA
J Struct Funct Genomics 6:159-63. 2005..Functional expression in Escherichia coli and an automated two-column purification routine allow ongoing crystallization experiments and studies on receptor-bound ligands... - A novel microRNA mmu-miR-466h affects apoptosis regulation in mammalian cellsAliaksandr Druz
Biotechnology Core Laboratory National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 9000 Rockville Pike Bldg 14A Rm 176, Bethesda, Maryland 20892, USA
Biotechnol Bioeng 108:1651-61. 2011..This suggests the pro-apoptotic role of mmu-miR-466h and its capability to modulate the apoptotic pathway in mammalian cells... - Production, purification, and characterization of human alpha1 proteinase inhibitor from Aspergillus nigerLiat Chill
Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 14A Room 170, 9000 Rockville Pike, Bethesda, Maryland 20892, USA
Biotechnol Bioeng 102:828-44. 2009..The Galactofuranose-containing high-mannnose type N-glycans are typical structures, which recently have been found as part of several glycoproteins produced by Aspergillus niger... - Screen-less expanded bed column: new approach for the recovery and purification of a malaria transmission blocking vaccine candidate from Pichia pastorisLoc Trinh
Biotechnology Unit, NIDDK, NIH Bldg 14A, Room 173, Bethesda, MD 20892, USA
Biotechnol Lett 28:951-8. 2006..The screen-less design did not affect the binding capacity, purification level or process yield and, therefore, shorten the process. Purified Pfs25H of 6.4 g were recovered from 37 l of Pichia pastoris culture in one step... - Glucose depletion activates mmu-miR-466h-5p expression through oxidative stress and inhibition of histone deacetylationAliaksandr Druz
Biotechnology Core Laboratory National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Health Bldg 14A Bethesda, MD 20892, USA
Nucleic Acids Res 40:7291-302. 2012..Improved characterization of the molecular events that lead to the activation of miR-466h-5p may provide a better understanding of the relation between cellular environment and miRNA activation... - Modified Escherichia coli B (BL21), a superior producer of plasmid DNA compared with Escherichia coli K (DH5alpha)Je Nie Phue
Biotechnology Core Laboratory, NIDDK NIH Bethesda, Bldg 14A Room 173, Maryland 20892, USA
Biotechnol Bioeng 101:831-6. 2008..The produced plasmid, which was constructed for HIV clinical study, was found to have identical properties to the plasmid currently produced by E. coli DH5alpha... - Long-lasting and transmission-blocking activity of antibodies to Plasmodium falciparum elicited in mice by protein conjugates of Pfs25Joanna Kubler Kielb
Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health, 31 Center Drive, MSC 2423, Bethesda, MD 20892 2520, USA
Proc Natl Acad Sci U S A 104:293-8. 2007..The observed transmission-blocking activity of immune sera correlated with antibody levels measured by ELISA... - Evaluation of production parameters with the vaccinia virus expression system using microcarrier attached HeLa cellsNicole A Bleckwenn
Biotechnology Unit, NIDDK, National Institutes of Health, DHHS, 9000 Rockville Pike, Bethesda, Maryland 20892, USA
Biotechnol Prog 21:554-61. 2005..The level of production achieved with this system reached approximately 17 microg EGFP/10(6) infected cells... - Egr1 and Gas6 facilitate the adaptation of HEK-293 cells to serum-free media by conferring enhanced viability and higher growth ratesPratik Jaluria
National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 9000 Rockville Pike, Biotechnology Unit, Building 14A, Room 173, Bethesda, Maryland 20892, USA
Biotechnol Bioeng 99:1443-52. 2008..These results indicate that enhanced expression of egr1 or gas6 facilitate adaptation to SFM by improving growth and viability... - Synthesis, characterization, and immunogenicity in mice of Shigella sonnei O-specific oligosaccharide-core-protein conjugatesJohn B Robbins
National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 106:7974-8. 2009..IgG antibody levels induced in young outbred mice by the S. sonnei O-SPC conjugates were significantly higher then those elicited by the O-SP conjugates. Accordingly, we propose to evaluate clinically these conjugates... - Phase 1 study of a recombinant mutant protective antigen of Bacillus anthracisJoseph A Bellanti
Program in Developmental and Molecular Immunity, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
Clin Vaccine Immunol 19:140-5. 2012..There were too few AVA recipients for statistical comparisons, but their antibody levels followed those of rPA. Anti-rPA measured by ELISA correlated with TNA titers (r = 0.97). These data support studying alum-adsorbed rPA in children... - Glucose uptake regulation in E. coli by the small RNA SgrS: comparative analysis of E. coli K-12 (JM109 and MG1655) and E. coli B (BL21)Alejandro Negrete
Biotechnology Core Laboratory, NIDDK, NIH, Bethesda, MD, USA
Microb Cell Fact 9:75. 2010..The increased level of sRNA SgrS reduces the level of the ptsG mRNA and consequently lowers the level of the glucose transporter IICBGlc. The suggested trigger for this action is the accumulation of the corresponding phospho-sugars... - Dimerization of the class A G protein-coupled neurotensin receptor NTS1 alters G protein interactionJim F White
Membrane Protein Structure and Function Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 104:12199-204. 2007..NTS1 monomers activate G alpha q beta(1)gamma(2), whereas receptor dimers catalyze nucleotide exchange with lower affinity. Our results demonstrate that NTS1 dimerization per se is not a prerequisite for G protein activation... - Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from Pichia pastorisL B Trinh
Biotechnology Unit, National Institutes of Health, NIDDK Building 6, Room B1-33, Bethesda, Maryland 20892-2715, USA
Biotechnol Bioeng 82:438-44. 2003.... - Structure of the agonist-bound neurotensin receptorJim F White
Membrane Protein Structure Function Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Department of Health and Human Services, Rockville, Maryland 20852, USA
Nature 490:508-13. 2012.... - Production and antigenic properties of influenza virus from suspension MDCK-siat7e cells in a bench-scale bioreactorChia Chu
National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
Vaccine 28:7193-201. 2010..As a proof-of-concept this work demonstrates that by using a proper strategy, high yields of biologically active hemagglutinin can be produced from scalable cultures of suspension MDCK-siat7e cells... - The molecular structure of the Toll-like receptor 3 ligand-binding domainJessica K Bell
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 102:10976-80. 2005..The TLR3-ECD structure indicates how LRR loops can establish distinct pathogen recognition receptors... - Bacillus anthracis cell wall produces injurious inflammation but paradoxically decreases the lethality of anthrax lethal toxin in a rat modelXizhong Cui
Critical Care Medicine Department, Clinical Center, National Institutes of Health, Building 10, Room 2C148, Bethesda, MD 20892, USA
Intensive Care Med 36:148-56. 2010..We therefore investigated these effects in rats and, for comparison, those of known inflammatory stimulants, Staphylococcus aureus cell wall or lipopolysaccharide (LPS)... - Mass spectrometry analysis of recombinant human ZP3 expressed in glycosylation-deficient CHO cellsMing Zhao
Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
Biochemistry 43:12090-104. 2004..Taken together, these data indicate that human and mouse ZP3 proteins are quite similar, and alternative explanations of taxon-specific sperm binding warrant exploration... - Conjugates of group A and W135 capsular polysaccharides of neisseria meningitidis bound to recombinant Staphylococcus aureus enterotoxin C1: preparation, physicochemical characterization, and immunological properties in miceZhigang Jin
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Infect Immun 73:7887-93. 2005..A bivalent formulation of GAMP-rSEC and W135P-rSEC elicited IgG anti-CP at comparable levels to those induced by the conjugates administered separately... - Reducing acetate excretion from E. coli K-12 by over-expressing the small RNA SgrSAlejandro Negrete
Biotechnology Core Laboratory, NIDDK, NIH, Bethesda, MD, USA
N Biotechnol 30:269-73. 2013..coli B. This observation opens a new approach towards controlling bacterial metabolism through the use of noncoding RNA... - Exploring vaccinia virus as a tool for large-scale recombinant protein expressionNicole A Bleckwenn
Biotechnology Unit, LCDB, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
Biotechnol Prog 19:130-6. 2003..2 microg EGFP/(million cells at the time of infection), demonstrating the ability of this system to successfully express recombinant proteins at larger scale... - The rise in pertussis cases urges replacement of chemically-inactivated with genetically-inactivated toxoid for DTPJohn B Robbins
National Institute of Child Health and Human Development, National Institutes of Health, 31 Center Drive, Bethesda, MD 20892 2423, USA
Vaccine 25:2811-6. 2007..Manufacturers have declined to produce the genetically-inactivated PTx because of the expense required to change to the improved vaccine and not because of scientific issues... - Endostatin capture from Pichia pastoris culture in a fluidized bed. From on-chip process optimization to applicationJoseph Shiloach
Biotechnology Unit, NIDDK, Bethesda, MD, USA
J Chromatogr B Analyt Technol Biomed Life Sci 790:327-36. 2003.... - Transcription levels of key metabolic genes are the cause for different glucose utilization pathways in E. coli B (BL21) and E. coli K (JM109)Je-Nie Phue
Biotechnology Unit, NIH, NIDDK, Building 14A Rm 173, Bethesda, MD 20892, USA
J Biotechnol 109:21-30. 2004..The transcript of acs is higher in E. coli BL21 and lower in the E. coli JM109, while the reverse is true for poxB transcription... - Automated large-scale purification of a G protein-coupled receptor for neurotensinJim F White
National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892-8030, USA
FEBS Lett 564:289-93. 2004..The presented expression and purification procedures are simple and robust, and provide the basis for crystallization experiments of receptors on a routine basis... - Dequalinium, a new inhibitor of Mycobacterium tuberculosis mycothiol ligase identified by high-throughput screeningMaria Teresa Gutierrez-Lugo
Laboratory of Bioorganic Chemistry, Biotechnology Unit, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20817, USA
J Biomol Screen 14:643-52. 2009..2 and 0.3 microg/mL, respectively. The screening protocol described is robust and has enabled the identification of new MshC inhibitors... - MKR mice are resistant to the metabolic actions of both insulin and adiponectin: discordance between insulin resistance and adiponectin responsivenessChul Hee Kim
Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
Am J Physiol Endocrinol Metab 291:E298-305. 2006..MKR mice may be a useful model for dissecting relationships between insulin resistance and adiponectin action in regulation of glucose homeostasis... - Development of a novel vaccinia-neutralization assay based on reporter-gene expressionJody Manischewitz
Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drugs Administration, Bethesda, Maryland 20892, USA
J Infect Dis 188:440-8. 2003..The new assay will serve as an important tool both for preclinical and clinical trials of new smallpox vaccines and for evaluation of therapeutic agents to treat vaccine-associated adverse reactions...