Genomes and Genes
J R Sellers
Affiliation: National Institutes of Health
- Myosins: a diverse superfamilyJ R Sellers
National Heart, Lung and Blood Institute, National Institutes of Health, Building 10, Room 8N202, Bethesda, MD 20892, USA
Biochim Biophys Acta 1496:3-22. 2000..This review will focus on myosins from class IV, VI, VII, VIII, X, XI, XII, XIII, XIV and XV. In addition, the function of myosin II in non-muscle cells will also be discussed...
- Unconventional myosins and the genetics of hearing lossT B Friedman
Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, Rockville, MD 20854, USA
Am J Med Genet 89:147-57. 1999..This review focuses on what we know about the molecular genetics and biochemistry of myosins VI, VIIA and XV as relates to hereditary hearing loss. Am. J. Med. Genet. (Semin. Med. Genet.) 89:147-157, 1999. Published 2000 Wiley-Liss, Inc...
- Xenopus nonmuscle myosin heavy chain isoforms have different subcellular localizations and enzymatic activitiesC A Kelley
Laboratory of Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
J Cell Biol 134:675-87. 1996..The different subcellular localizations of MHC-A and MHC-B, together with the data demonstrating that these myosins have markedly different enzymatic activities, strongly suggests that they have different functions...
- Identification and analysis of the myosin superfamily in Drosophila: a database approachR A Yamashita
Laboratory of Molecular Cardiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA
J Muscle Res Cell Motil 21:491-505. 2000..In the future comparative genomics will hopefully lead to the placement of these myosins into new classes...
- Human myosin XVBP is a transcribed pseudogeneE T Boger
Laboratory of Molecular Genetics, Section on Human Genetics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Rockville, Maryland 20850, USA
J Muscle Res Cell Motil 22:477-83. 2001..Analyses of these myosin XVBP cDNAs revealed numerous additional disablements including translational reading frame shifts resulting in stop codons. From these data we conclude that myosin XVBP is a transcribed, unprocessed pseudogene...
- Skeletal muscle expression and abnormal function of beta-myosin in hypertrophic cardiomyopathyG Cuda
Laboratory of Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892
J Clin Invest 91:2861-5. 1993..Thus, single amino acid changes in beta-myosin result in abnormal actomyosin interactions, confirming the primary role of missense mutations in beta-MHC gene in the etiology of hypertrophic cardiomyopathy...
- In vitro motility assay to study translocation of actin by myosinJ R Sellers
National Heart, Lung, and Blood Institute, Bethesda, Maryland, USA
Curr Protoc Cell Biol . 2001..The system is fast, easy to set up and maintain, uses only small amounts of protein, and yields quantitative results...
- Molecular characterization of myosin V from Drosophila melanogasterN Bonafe
National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1762, USA
J Muscle Res Cell Motil 19:129-41. 1998..While vertebrates would need two different myosin V isoforms to accomplish specific functions, we speculate that Drosophila myosin V might provide the equivalent functions by itself...
- Actin-dependent motility of melanosomes from fish retinal pigment epithelial (RPE) cells investigated using in vitro motility assaysE L McNeil
Department of Biology, Saint Joseph s University, Philadelphia, Pennsylvania 19131, USA
Cell Motil Cytoskeleton 58:71-82. 2004..Immunoblotting using antibodies against myosin VIIa and rab27a revealed that both proteins are enriched on melanosome membranes, suggesting that they could play a role in melanosome transport within apical projections of fish RPE...
- Characterization of the human and mouse unconventional myosin XV genes responsible for hereditary deafness DFNB3 and shaker 2Y Liang
Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders NIDCD, National Institutes of Health NIH, 5 Research Court, Rockville, Maryland, 20850, USA
Genomics 61:243-58. 1999..These results indicate a likely role for myosin XV in the formation or maintenance of the unique actin-rich structures of inner ear sensory hair cells...
- Effect of Mts1 on the structure and activity of nonmuscle myosin IIH L Ford
Department of Biochemistry and Cancer Center, University of Rochester, 601 Elmwood Avenue, Rochester, New York 14642, USA
Biochemistry 36:16321-7. 1997..The data demonstrate an effect of Mts1 on both myosin structure and function, and suggest a route through which Mts1 affects motility as well as metastasis...
- Mutations in either the essential or regulatory light chains of myosin are associated with a rare myopathy in human heart and skeletal muscleK Poetter
Inherited Cardiac Diseases Section, NHLBI, NIH Bethesda, Maryland 20892 1650, USA
Nat Genet 13:63-9. 1996....
- Structural interpretation of the mutations in the beta-cardiac myosin that have been implicated in familial hypertrophic cardiomyopathyI Rayment
Institute for Enzyme Research, Graduate School, University of Wisconsin, Madison 53705 4098, USA
Proc Natl Acad Sci U S A 92:3864-8. 1995..The remaining five mutations are in the myosin rod. The locations of these mutations provide insight into the way they impair the functioning of this molecular motor and also into the mechanism of energy transduction...