P Schuck

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Regulation of ASAP1 by phospholipids is dependent on the interface between the PH and Arf GAP domains
    Magnus M Che
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, United States
    Cell Signal 17:1276-88. 2005
  2. pmc Size-distribution analysis of proteins by analytical ultracentrifugation: strategies and application to model systems
    Peter Schuck
    Division of Bioengineering and Physical Science, Office of Research Services, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, 20892, USA
    Biophys J 82:1096-111. 2002
  3. pmc On the analysis of sedimentation velocity in the study of protein complexes
    Patrick H Brown
    Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892 5766, USA
    Eur Biophys J 38:1079-99. 2009
  4. pmc Kinetics of ligand binding to receptor immobilized in a polymer matrix, as detected with an evanescent wave biosensor. I. A computer simulation of the influence of mass transport
    P Schuck
    Laboratory of Biochemical Pharmacology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institute of Health, Bethesda, Maryland 20892, USA
    Biophys J 70:1230-49. 1996
  5. ncbi request reprint On the analysis of protein self-association by sedimentation velocity analytical ultracentrifugation
    Peter Schuck
    Protein Biophysics Resource, Division of Bioengineering and Physical Science, ORS, OD, National Institutes of Health, Bethesda, MD 20892, USA
    Anal Biochem 320:104-24. 2003
  6. ncbi request reprint A model for sedimentation in inhomogeneous media. I. Dynamic density gradients from sedimenting co-solutes
    Peter Schuck
    Division of Bioengineering and Physical Science, ORS, OD, National Institutes of Health, Building 13, Room 3N17, 13 South Drive, Bethesda, MD 20892 5766, USA
    Biophys Chem 108:187-200. 2004
  7. ncbi request reprint Analysis of transport experiments using pseudo-absorbance data
    S R Kar
    Molecular Interactions Resource, National Institutes of Health, Bethesda, Maryland 20892, USA
    Anal Biochem 285:135-42. 2000
  8. ncbi request reprint Determination of the sedimentation coefficient distribution by least-squares boundary modeling
    P Schuck
    Molecular Interactions Resource, Bioengineering and Physical Science Program, ORS, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biopolymers 54:328-41. 2000
  9. ncbi request reprint A model for sedimentation in inhomogeneous media. II. Compressibility of aqueous and organic solvents
    Peter Schuck
    Division of Bioengineering and Physical Science, ORS, OD, National Institutes of Health, Building 13, Rm 3N17, 13 South Drive, Bethesda, MD 20892 5766, USA
    Biophys Chem 108:201-14. 2004
  10. pmc Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and lamm equation modeling
    P Schuck
    Molecular Interactions Resource, Bioengineering and Physical Science Program, ORS, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biophys J 78:1606-19. 2000

Detail Information

Publications91

  1. ncbi request reprint Regulation of ASAP1 by phospholipids is dependent on the interface between the PH and Arf GAP domains
    Magnus M Che
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, United States
    Cell Signal 17:1276-88. 2005
    ....
  2. pmc Size-distribution analysis of proteins by analytical ultracentrifugation: strategies and application to model systems
    Peter Schuck
    Division of Bioengineering and Physical Science, Office of Research Services, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, 20892, USA
    Biophys J 82:1096-111. 2002
    ..Although c(s) is computationally the most complex, it has the potential for the highest resolution and sensitivity of the methods described...
  3. pmc On the analysis of sedimentation velocity in the study of protein complexes
    Patrick H Brown
    Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892 5766, USA
    Eur Biophys J 38:1079-99. 2009
    ..We describe how these effects can be computationally accounted for in SEDFIT and SEDPHAT...
  4. pmc Kinetics of ligand binding to receptor immobilized in a polymer matrix, as detected with an evanescent wave biosensor. I. A computer simulation of the influence of mass transport
    P Schuck
    Laboratory of Biochemical Pharmacology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institute of Health, Bethesda, Maryland 20892, USA
    Biophys J 70:1230-49. 1996
    ..The use of a thinner polymer matrix could significantly increase the range of detectable rate constants...
  5. ncbi request reprint On the analysis of protein self-association by sedimentation velocity analytical ultracentrifugation
    Peter Schuck
    Protein Biophysics Resource, Division of Bioengineering and Physical Science, ORS, OD, National Institutes of Health, Bethesda, MD 20892, USA
    Anal Biochem 320:104-24. 2003
    ..Further, under suitable conditions, fractions of incompetent material that are not part of the reversible equilibrium can be detected...
  6. ncbi request reprint A model for sedimentation in inhomogeneous media. I. Dynamic density gradients from sedimenting co-solutes
    Peter Schuck
    Division of Bioengineering and Physical Science, ORS, OD, National Institutes of Health, Building 13, Room 3N17, 13 South Drive, Bethesda, MD 20892 5766, USA
    Biophys Chem 108:187-200. 2004
    ..General features of protein sedimentation in dynamic density gradients are described...
  7. ncbi request reprint Analysis of transport experiments using pseudo-absorbance data
    S R Kar
    Molecular Interactions Resource, National Institutes of Health, Bethesda, Maryland 20892, USA
    Anal Biochem 285:135-42. 2000
    ..It is shown that pseudo-absorbance analysis can increase the capacity of a sedimentation velocity experiment in ultracentrifugation, and, in general, can considerably simplify the requirements of optical design...
  8. ncbi request reprint Determination of the sedimentation coefficient distribution by least-squares boundary modeling
    P Schuck
    Molecular Interactions Resource, Bioengineering and Physical Science Program, ORS, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biopolymers 54:328-41. 2000
    ..Because the method does not require a high frequency of scans, it can also be easily used in experiments with the absorbance optical scanning system. Published 2000 John Wiley & Sons, Inc...
  9. ncbi request reprint A model for sedimentation in inhomogeneous media. II. Compressibility of aqueous and organic solvents
    Peter Schuck
    Division of Bioengineering and Physical Science, ORS, OD, National Institutes of Health, Building 13, Rm 3N17, 13 South Drive, Bethesda, MD 20892 5766, USA
    Biophys Chem 108:201-14. 2004
    ..The consideration of solvent compressibility is highly relevant not only when using organic solvents, but also in aqueous solvents when precise sedimentation coefficients are needed, for example, for hydrodynamic modeling...
  10. pmc Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and lamm equation modeling
    P Schuck
    Molecular Interactions Resource, Bioengineering and Physical Science Program, ORS, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biophys J 78:1606-19. 2000
    ....
  11. pmc Direct sedimentation analysis of interference optical data in analytical ultracentrifugation
    P Schuck
    Molecular Interactions Resource, Bioengineering and Physical Science Program, ORS, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biophys J 76:2288-96. 1999
    ..They can be extracted from data acquired during the approach to equilibrium, and can be used to increase the reliability of the results obtained from a sedimentation equilibrium analysis...
  12. ncbi request reprint Kinetic analysis of biosensor data: elementary tests for self-consistency
    P Schuck
    Section of Physical Biochemistry, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Trends Biochem Sci 21:458-60. 1996
    ..A search of the recent literature reveals that many published results fail these tests qualitatively...
  13. ncbi request reprint Use of surface plasmon resonance to probe the equilibrium and dynamic aspects of interactions between biological macromolecules
    P Schuck
    Section of Physical Biochemistry, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Biophys Biomol Struct 26:541-66. 1997
    ..Described here is the influence of these artifacts on the measured binding kinetics and equilibria, together with suggested control experiments...
  14. ncbi request reprint Reliable determination of binding affinity and kinetics using surface plasmon resonance biosensors
    P Schuck
    Biomedical Engineering and Instrumentation Program, National Institutes of Health, Bethesda, MD 20892 5766, USA
    Curr Opin Biotechnol 8:498-502. 1997
    ..Improved analytical approaches include the application of global analysis and analytical corrections for the influence of mass transport...
  15. pmc Determination of sedimentation coefficients for small peptides
    P Schuck
    Biomedical Engineering and Instrumentation Program, ORS, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biophys J 74:466-74. 1998
    ....
  16. ncbi request reprint Rapid determination of molar mass in modified Archibald experiments using direct fitting of the Lamm equation
    P Schuck
    Molecular Interactions Resource, BEPS, OD, National Institutes of Health, Bethesda, Maryland 20892, USA
    Anal Biochem 259:48-53. 1998
    ....
  17. pmc Sedimentation analysis of noninteracting and self-associating solutes using numerical solutions to the Lamm equation
    P Schuck
    Bioengineering and Physical Science Program, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biophys J 75:1503-12. 1998
    ..In addition, in the case of a protein in rapid monomer-dimer equilibrium, this configuration was found to reveal the most precise estimate of the association constant...
  18. ncbi request reprint Determination of binding constants by equilibrium titration with circulating sample in a surface plasmon resonance biosensor
    P Schuck
    Molecular Interactions Resource, Bioengineering and Physical Science Program, ORS, National Institutes of Health, Bethesda, Maryland 20892, USA
    Anal Biochem 265:79-91. 1998
    ..The rapid injection of high concentrations of competitor allows the observation of fast dissociation processes under conditions minimizing rebinding...
  19. ncbi request reprint Rotavirus nonstructural protein NSP2 self-assembles into octamers that undergo ligand-induced conformational changes
    P Schuck
    Division of Bioengineering and Physical Science, ORS, OD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:9679-87. 2001
    ..Chen, D., and Patton, J. T. (1999) J. Virol. 73, 9934-9943) of its function as a molecular motor involved in the packaging of viral mRNA...
  20. pmc Bayesian analysis of heterogeneity in the distribution of binding properties of immobilized surface sites
    Inna I Gorshkova
    Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892, USA
    Langmuir 24:11577-86. 2008
    ..The distributions obtained are highly reproducible. Immobilization conditions and the total surface density of immobilized sites can have a substantial impact on the functional distribution of the binding sites...
  21. pmc Sedimentation velocity analysis of heterogeneous protein-protein interactions: Lamm equation modeling and sedimentation coefficient distributions c(s)
    Julie Dam
    Center for Advanced Research in Biotechnology, W M Keck Laboratory for Structural Biology, University of Maryland Biotechnology Institute, Rockville, Maryland, USA
    Biophys J 89:619-34. 2005
    ..The integration of the c(s) peaks can lead to isotherms of species populations or s-value of the reaction boundary, respectively, which can be used for the determination of the equilibrium binding constant...
  22. pmc Sedimentation velocity analysis of heterogeneous protein-protein interactions: sedimentation coefficient distributions c(s) and asymptotic boundary profiles from Gilbert-Jenkins theory
    Julie Dam
    Center for Advanced Research in Biotechnology, W M Keck Laboratory for Structural Biology, University of Maryland Biotechnology Institute, Rockville, Maryland, USA
    Biophys J 89:651-66. 2005
    ..This can significantly improve the estimates for the determination of binding constants and hydrodynamic parameters of the complexes...
  23. pmc Macromolecular size-and-shape distributions by sedimentation velocity analytical ultracentrifugation
    Patrick H Brown
    Protein Biophysics Resource, Division of Bioengineering and Physical Science, ORS, Office of the Director, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biophys J 90:4651-61. 2006
    ..We show that this can be used to determine average molar masses of macromolecules and characterize macromolecular distributions, without the approximation of any scaling relationship between hydrodynamic and thermodynamic parameters...
  24. ncbi request reprint Calcium-sensitive interaction between calmodulin and modified forms of rat brain neurogranin/RC3
    K P Huang
    Section on Metabolic Regulation, National Institutes of Health, Bethesda, MD 20892 4510, USA
    Biochemistry 39:7291-9. 2000
    ..These results indicate that modification of NG to form intramolecular disulfides outside the IQ domain provides an alternative mechanism for regulation of its binding affinity to CaM...
  25. ncbi request reprint Adaptation of a surface plasmon resonance biosensor with microfluidics for use with small sample volumes and long contact times
    M Abrantes
    Molecular Interactions Resource, Division of Bioengineering and Physical Science, ORS, OD, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Anal Chem 73:2828-35. 2001
    ..This can translate to improved detection limits for slow reactions and can facilitate kinetic and thermodynamic binding studies...
  26. pmc Novel chimpanzee/human monoclonal antibodies that neutralize anthrax lethal factor, and evidence for possible synergy with anti-protective antigen antibody
    Zhaochun Chen
    Laboratory of Infectious Diseases1 and Laboratory of Bacterial Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Infect Immun 77:3902-8. 2009
    ..Although two unique MAbs could protect against anthrax when used alone, even more efficient and broader protection should be gained by combining them with anti-PA MAbs...
  27. ncbi request reprint Self-association and ligand-induced conformational changes of iron regulatory proteins 1 and 2
    Emine Yikilmaz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 5766, USA
    Biochemistry 44:8470-8. 2005
    ..The dynamic change of the IRP between different oligomeric and conformational states induced by interaction with IRE may play a role in the iron regulatory functions of IRPs...
  28. pmc Chimpanzee/human mAbs to vaccinia virus B5 protein neutralize vaccinia and smallpox viruses and protect mice against vaccinia virus
    Zhaochun Chen
    Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Office of Research Services, Office of the Director, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:1882-7. 2006
    ..These chimpanzee/human anti-B5 mAbs may be useful in the prevention and treatment of vaccinia virus-induced complications of vaccination against smallpox and may also be effective in the immunoprophylaxis and immunotherapy of smallpox...
  29. ncbi request reprint Sedimentation equilibrium analysis of protein interactions with global implicit mass conservation constraints and systematic noise decomposition
    Jennifer Vistica
    Protein Biophysics Resource, Division of Bioengineering and Physical Science, ORS, OD, National Institutes of Health, Bethesda, MD 20892, USA
    Anal Biochem 326:234-56. 2004
    ..The properties of these tools are studied with theoretical and experimental data sets...
  30. pmc Using prior knowledge in the determination of macromolecular size-distributions by analytical ultracentrifugation
    Patrick H Brown
    Protein Biophysics Resource, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biomacromolecules 8:2011-24. 2007
    ..Further, the use of multiple alternative prior information allows us to probe the range of possible interpretations consistent with the data...
  31. ncbi request reprint Alanine-scanning mutations in domain 4 of anthrax toxin protective antigen reveal residues important for binding to the cellular receptor and to a neutralizing monoclonal antibody
    M J Rosovitz
    Microbial Pathogenesis Section, NIAID, National Institutes of Health, Bethesda, Maryland 20892 4350, USA
    J Biol Chem 278:30936-44. 2003
    ..The K684A and L685A variants exhibited wild type levels of toxicity in cell culture assays; the L687A and Y688A variants were reduced only 1.5- and 5-fold, respectively...
  32. doi request reprint Characterizing protein-protein interactions by sedimentation velocity analytical ultracentrifugation
    Patrick H Brown
    National Institutes of Health, Bethesda, Maryland, USA
    Curr Protoc Immunol . 2008
    ..Concepts for the experimental design are discussed, and a detailed step-by-step protocol guiding the reader through the experiment and the data analysis is available as an Internet resource...
  33. ncbi request reprint Measuring protein interactions by optical biosensors
    P Schuck
    National Institutes of Health, Bethesda, Maryland, USA
    Curr Protoc Protein Sci . 2001
    ..Emphasis is given to the description of simple and robust approaches that will provide reliable results. How to avoid the most commonly encountered problems and artifacts is also discussed...
  34. pmc Combined affinity and rate constant distributions of ligand populations from experimental surface binding kinetics and equilibria
    Juraj Svitel
    Division of Bioengineering and Physical Science, ORS, Office of the Director, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biophys J 84:4062-77. 2003
    ..The distribution analysis can provide a rational interpretation of complex experimental surface binding kinetics, and provides an analytical tool for probing the homogeneity of the populations of immobilized protein...
  35. ncbi request reprint Measuring protein-protein interactions by equilibrium sedimentation
    Andrea Balbo
    National Institutes of Health, Bethesda, Maryland, USA
    Curr Protoc Immunol . 2007
    ..A detailed protocol guiding the investigator through the experimental steps and the data analysis is available as an internet resource...
  36. ncbi request reprint Hydrodynamics of nanoscopic tubulin rings in dilute solutions
    Hacene Boukari
    Laboratory of Integrative and Medical Biophysics, NICHD, Bethesda, Maryland 20892, USA
    Phys Rev Lett 93:098106. 2004
    ..We use the high-precision ratio of the sedimentation coefficients and that of the translational diffusion coefficients to validate theories for calculating the hydrodynamic properties of supramolecular structures...
  37. doi request reprint Measuring protein interactions by optical biosensors
    Peter Schuck
    National Institutes of Health, Bethesda, Maryland, USA
    Curr Protoc Cell Biol . 2004
    ..This unit provides a thorough, well-illustrated discussion of the principles of optical biosensors, experimental design, ligand immobilization, experimental data analysis, and common obstacles and possible solutions...
  38. doi request reprint Accounting for solvent signal offsets in the analysis of interferometric sedimentation velocity data
    Huaying Zhao
    Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, NIBIB, National Institutes of Health, Bethesda, Maryland, USA
    Macromol Biosci 10:736-45. 2010
    ..We demonstrate how this can restore the SV analysis to yield a high quality fit of the data and to provide correct macromolecular sedimentation parameters...
  39. pmc A bayesian approach for quantifying trace amounts of antibody aggregates by sedimentation velocity analytical ultracentrifugation
    Patrick H Brown
    Dynamics of Macromolecular Assembly Section, Laboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892 5766, USA
    AAPS J 10:481-93. 2008
    ..Implications for the experimental design of SV-AUC and practical detection limits are discussed...
  40. ncbi request reprint Single-walled tubulin ring polymers
    Hacene Boukari
    Laboratory of Integrative and Medical Biophysics, NICHD, National Institutes of Health, Bethesda, MD 20892, USA
    Biopolymers 86:424-36. 2007
    ..Moreover, we show that, by using several techniques, one can eliminate degeneracy to provide better discrimination between model structures...
  41. pmc Humanized monoclonal antibodies derived from chimpanzee Fabs protect against Japanese encephalitis virus in vitro and in vivo
    Ana P Goncalvez
    Molecular Viral Biology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
    J Virol 82:7009-21. 2008
    ....
  42. pmc Some statistical properties of differencing schemes for baseline correction of sedimentation velocity data
    Peter Schuck
    Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA
    Anal Biochem 401:280-7. 2010
    ....
  43. pmc Sedimentation patterns of rapidly reversible protein interactions
    Peter Schuck
    Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
    Biophys J 98:2005-13. 2010
    ..Adding to the existing numerical solutions of the relevant partial differential equations, the effective particle model provides physical insights into the relationships of the parameters that govern sedimentation patterns...
  44. pmc Two interferons alpha influence each other during their interaction with the extracellular domain of human type interferon receptor subunit 2
    Hana Schmeisser
    National Institute of Allergy and Infectious Diseases and National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 46:14638-49. 2007
    ..This study contributes to the understanding of the mutual interaction between multiple IFN-alpha subtypes during the competition for binding to the receptor...
  45. pmc On computational approaches for size-and-shape distributions from sedimentation velocity analytical ultracentrifugation
    Peter Schuck
    Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, National Institutes of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA
    Eur Biophys J 39:1261-75. 2010
    ..While the 2DSA appears computationally excessively wasteful, key elements also appear to be in conflict with mathematical results. This raises doubts about the correctness of the results from 2DSA analysis...
  46. pmc Characterization of chimpanzee/human monoclonal antibodies to vaccinia virus A33 glycoprotein and its variola virus homolog in vitro and in a vaccinia virus mouse protection model
    Zhaochun Chen
    Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, NIH, 50 South Drive, MSC 8009, Bethesda, MD 20892, USA
    J Virol 81:8989-95. 2007
    ....
  47. pmc Diffusion of the reaction boundary of rapidly interacting macromolecules in sedimentation velocity
    Peter Schuck
    Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
    Biophys J 98:2741-51. 2010
    ....
  48. ncbi request reprint Grafting of "abbreviated" complementarity-determining regions containing specificity-determining residues essential for ligand contact to engineer a less immunogenic humanized monoclonal antibody
    Roberto De Pascalis
    Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 169:3076-84. 2002
    ..The final variant of the HuCOL-1, which retains its Ag-binding reactivity and shows significantly lower serum reactivity than that of the parental Ab, can serve as a prototype for the development of a potentially useful clinical reagent...
  49. pmc Modern analytical ultracentrifugation in protein science: a tutorial review
    Jacob Lebowitz
    Molecular Interactions Resource, Division of Bioengineering and Physical Science, ORS, OD, National Institutes of Health, Bethesda, Maryland 20892, USA
    Protein Sci 11:2067-79. 2002
    ..This is the method of choice for molar mass determinations and the study of self-association and heterogeneous interactions, such as protein-protein, protein-nucleic acid, and protein-small molecule binding...
  50. ncbi request reprint Binding specificity of multiprotein signaling complexes is determined by both cooperative interactions and affinity preferences
    Jon C D Houtman
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 43:4170-8. 2004
    ..These studies provide quantitative information on how different binding parameters can determine in vivo binding site specificity observed for multiprotein signaling complexes...
  51. ncbi request reprint Efficient neutralization of anthrax toxin by chimpanzee monoclonal antibodies against protective antigen
    Zhaochun Chen
    Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Infect Dis 193:625-33. 2006
    ..These data suggest that W1 and W2 chimpanzee monoclonal antibodies may serve as PA entry inhibitors for use in the emergency prophylaxis against and treatment of anthrax...
  52. ncbi request reprint Biochemical and biological characterization of a dodecameric CD4-Ig fusion protein: implications for therapeutic and vaccine strategies
    James Arthos
    Laboratory of Immunoregulation, NIAID, and the Molecular Interactions Resource Division of Bioengineering and Physical Science, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:11456-64. 2002
    ..This protein does not enhance viral replication at suboptimal concentrations. These observations may aid in the design of new therapeutics and vaccines...
  53. ncbi request reprint Inhibition of hemostasis by a high affinity biogenic amine-binding protein from the saliva of a blood-feeding insect
    John F Andersen
    Laboratory of Malaria and Vector Research, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 278:4611-7. 2003
    ..Molecular modeling of ABP suggests that ligand binding is mediated by interaction with the side chains of aromatic amino acids and charged residues that line the binding pocket...
  54. pmc Autoinhibition of Arf GTPase-activating protein activity by the BAR domain in ASAP1
    Xiaoying Jian
    Laboratory of Cellular and Molecular Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 284:1652-63. 2009
    ..Analysis of single turnover kinetics revealed a transition state intermediate that was affected by the BAR domain. We conclude that BAR domains can affect enzymatic activity through intraprotein interactions...
  55. pmc Energetics of glutamate receptor ligand binding domain dimer assembly are modulated by allosteric ions
    Charu Chaudhry
    Laboratory of Cellular and Molecular Neurophysiology, Porter Neuroscience Research Center, National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:12329-34. 2009
    ....
  56. ncbi request reprint Elucidating kinetic and thermodynamic constants for interaction of G protein subunits and receptors by surface plasmon resonance spectroscopy
    R Victor Rebois
    Laboratory of Molecular and Cellular Neurobiology and Biology, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
    Methods Enzymol 344:15-42. 2002
  57. pmc Studying multiprotein complexes by multisignal sedimentation velocity analytical ultracentrifugation
    Andrea Balbo
    Protein Biophysics Resource, Division of Bioengineering and Physical Science, Office of Research Services, Office of the Director, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:81-6. 2005
    ....
  58. ncbi request reprint Crystal structure of an NK cell immunoglobulin-like receptor in complex with its class I MHC ligand
    J C Boyington
    Structural Biology Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA
    Nature 405:537-43. 2000
    ..A second KIR/HLA interface produced an ordered receptor-ligand aggregation in the crystal which may resemble receptor clustering during immune synapse formation...
  59. pmc Oligomeric structure of virion-associated and soluble forms of the simian immunodeficiency virus envelope protein in the prefusion activated conformation
    R J Center
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, and Division of Bioengineering and Physical Science, Office of Research Services, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 98:14877-82. 2001
    ..Furthermore, a triangular or tri-lobed morphology was clearly visualized in a subset of the trimers...
  60. ncbi request reprint Dissection of merozoite surface protein 3, a representative of a family of Plasmodium falciparum surface proteins, reveals an oligomeric and highly elongated molecule
    Brandt R Burgess
    Structural Biology Section, Laboratory of Immunogenetics, NIAID, National Institutes of Health, Rockville, Maryland 20852, USA
    J Biol Chem 280:37236-45. 2005
    ....
  61. pmc Eukaryotic RNases H1 act processively by interactions through the duplex RNA-binding domain
    Sergei A Gaidamakov
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development Bethesda, MD 20892, USA
    Nucleic Acids Res 33:2166-75. 2005
    ..In mitochondria, where RNase H1 is essential for DNA formation during embryogenesis, long hybrids may be involved in DNA replication...
  62. ncbi request reprint Mass spectrometry after capture and small-volume elution of analyte from a surface plasmon resonance biosensor
    John J Gilligan
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Anal Chem 74:2041-7. 2002
    ..Examples are presented for the specific detection and recovery of a protein from a complex mixture of cytosolic proteins...
  63. pmc Probing the functional heterogeneity of surface binding sites by analysis of experimental binding traces and the effect of mass transport limitation
    Juraj Svitel
    National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
    Biophys J 92:1742-58. 2007
    ..In the latter regime, we report experimental evidence for the formation of gradients within the sensing volume of the evanescent field biosensor used...
  64. doi request reprint The role of mass transport limitation and surface heterogeneity in the biophysical characterization of macromolecular binding processes by SPR biosensing
    Peter Schuck
    Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering NIBIB, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 627:15-54. 2010
    ..The effect of these factors on the observed binding kinetics, and strategies to account for them are reviewed, both in the context of mathematical data analysis, as well as the design of the experiments and controls...
  65. pmc Direct interaction of the mouse cytomegalovirus m152/gp40 immunoevasin with RAE-1 isoforms
    Li Zhi
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 1892, USA
    Biochemistry 49:2443-53. 2010
    ..Molecular modeling of the different RAE-1 isoforms suggests a potential site for the m152 interaction...
  66. ncbi request reprint Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgG
    Johnson Agniswamy
    Structural Immunology Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12441 Parklawn Drive, Rockville, Maryland 20852, USA
    Structure 14:225-35. 2006
    ..A tunnel observed at the dimer interface constitutes a target for designing potential Mac-1-specific antimicrobial agents. The structures also offer insight into the functional difference between Mac-1 and Mac-2...
  67. ncbi request reprint Surface plasmon resonance-based competition assay to assess the sera reactivity of variants of humanized antibodies
    Noreen R Gonzales
    Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 10 Center Drive, Room 8B09, Bethesda, MD 20892, USA
    J Immunol Methods 268:197-210. 2002
    ..This may facilitate the rapid screening, for the presence of anti-VR Abs, of the sera of patients undergoing clinical trials...
  68. pmc Analysis of a temperature-sensitive mutant rotavirus indicates that NSP2 octamers are the functional form of the protein
    Zenobia F Taraporewala
    Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Virol 76:7082-93. 2002
    ..The propensity of tsE NSP2 to form large aggregates provides a possible explanation for the inability of the protein to support packaging and/or replication in the infected cell at the nonpermissive temperature...
  69. doi request reprint Extended polypeptide linkers establish the spatial architecture of a pyruvate dehydrogenase multienzyme complex
    Jeffrey S Lengyel
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Structure 16:93-103. 2008
    ....
  70. pmc Cellular expression and crystal structure of the murine cytomegalovirus major histocompatibility complex class I-like glycoprotein, m153
    Janet Mans
    Molecular Biology Section, Laboratory of Immunology, NIAID, National Institutes of Health, Bethesda, Maryland 20892 1892, USA
    J Biol Chem 282:35247-58. 2007
    ..The structure provides a useful framework for comparative analysis of the divergent members of the m145 family...
  71. ncbi request reprint In vitro affinity maturation of a specificity-determining region-grafted humanized anticarcinoma antibody: isolation and characterization of minimally immunogenic high-affinity variants
    Roberto De Pascalis
    Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Clin Cancer Res 9:5521-31. 2003
    ..To generate variants of V10 with higher Ag-binding affinity and lower sera reactivity, in vitro affinity maturation of V10 was carried out using phage display technique...
  72. ncbi request reprint Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1
    Jon C D Houtman
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, US National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Struct Mol Biol 13:798-805. 2006
    ..These data suggest that multipoint binding of distal adaptor proteins mediates the formation of oligomeric signaling clusters vital for intracellular signaling...
  73. pmc Stability of ligand-binding domain dimer assembly controls kainate receptor desensitization
    Charu Chaudhry
    Laboratory of Cellular and Molecular Neurophysiology, Porter Neuroscience Research Center, NICHD, NIH, DHHS, Bethesda, MD, USA
    EMBO J 28:1518-30. 2009
    ..Our results show how neurotransmitter receptors with similar structures and gating mechanisms can exhibit strikingly different functional properties...
  74. ncbi request reprint Quantifying the energetics of cooperativity in a ternary protein complex
    Peter S Andersen
    Institute of Medical Microbiology and Immunology, University of Copenhagen, Blegdamsvej 3C, DK 2200 Copenhagen N, Denmark
    Biochemistry 41:5177-84. 2002
    ..Our experimental and theoretical analysis may be applicable to other systems involving cooperativity...
  75. pmc Cooperative behavior of Escherichia coli cell-division protein FtsZ assembly involves the preferential cyclization of long single-stranded fibrils
    Jose Manuel Gonzalez
    Centro de Investigaciones Biológicas and Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Cientificas, 28040 Madrid, Spain
    Proc Natl Acad Sci U S A 102:1895-900. 2005
    ..The proposed model is in accord with the results obtained from our experimental observations...
  76. ncbi request reprint Influence of temperature on the conformation of canine plasminogen: an analytical ultracentrifugation and dynamic light scattering study
    Jack A Kornblatt
    Enzyme Research Group, Department of Biology, Concordia University, 7141 Sherbrooke Ouest, Montreal, Quebec, Canada
    Biochemistry 44:13122-31. 2005
    ....
  77. ncbi request reprint Calculating sedimentation coefficient distributions by direct modeling of sedimentation velocity concentration profiles
    Julie Dam
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    Methods Enzymol 384:185-212. 2004
  78. pmc Studying multisite binary and ternary protein interactions by global analysis of isothermal titration calorimetry data in SEDPHAT: application to adaptor protein complexes in cell signaling
    Jon C D Houtman
    Department of Microbiology, University of Iowa, Iowa 52242, USA
    Protein Sci 16:30-42. 2007
    ....
  79. pmc Reversible and fast association equilibria of a molecular chaperone, gp57A, of bacteriophage T4
    Said A Ali
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan
    Biophys J 85:2606-18. 2003
    ..Trimer or hexamer is likely the functional oligomeric state of gp57A...
  80. pmc Bacteriorhodopsin/amphipol complexes: structural and functional properties
    Yann Gohon
    Laboratoire de Physico Chimie Moléculaire des Membranes Biologiques, Centre National de la Recherche Scientifique, and Université Paris 7, Institut de Biologie Physico Chimique, Paris, France
    Biophys J 94:3523-37. 2008
    ..In the absence of free APol, BR/APol particles can autoassociate into small or large ordered fibrils...
  81. ncbi request reprint Variable MHC class I engagement by Ly49 natural killer cell receptors demonstrated by the crystal structure of Ly49C bound to H-2K(b)
    Julie Dam
    Center for Advanced Research in Biotechnology, W M Keck Laboratory for Structural Biology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA
    Nat Immunol 4:1213-22. 2003
    ..We propose a dynamic model for Ly49-MHC class I interactions involving conformational changes in the receptor, whereby variations in Ly49 dimerization mediate different MHC-binding modes...
  82. ncbi request reprint Targeted lysis of HIV-infected cells by natural killer cells armed and triggered by a recombinant immunoglobulin fusion protein: implications for immunotherapy
    Neil Gupta
    Laboratory of Immunoregulation, NIAID, NIH Bldg 10 6A08, 9000 Rockville Pike, Bethesda MD 20892, USA
    Virology 332:491-7. 2005
    ..NK cells pre-armed in this manner retain the capacity to kill targets over an extended period of time. This strategy may have application to other disease states including various viral infections and cancers...
  83. pmc A mechanism for assembly of complexes of vitronectin and plasminogen activator inhibitor-1 from sedimentation velocity analysis
    Kenneth H Minor
    Department of Biochemistry, Cellular, and Molecular Biology and the Center of Excellence in Structural Biology, University of Tennessee, Knoxville, Tennessee 37996, USA
    J Biol Chem 280:28711-20. 2005
    ..This 2:1 PAI-1-vitronectin complex, with a sedimentation coefficient of 6.5 S, is the key intermediate for the assembly of higher order complexes...
  84. ncbi request reprint Differences in the binding capacity of human apolipoprotein E3 and E4 to size-fractionated lipid emulsions
    Matthew A Perugini
    Department of Biochemistry and Molecular Biology, The University of Melbourne, Parkville, Vic, Australia
    Eur J Biochem 269:5939-49. 2002
    ..Implications for the role of apoE4 in blood lipid transport and disease are discussed...
  85. ncbi request reprint The 'smallest real difference' as a measure of sensitivity to change: a critical analysis
    Peter Schuck
    Institute of Psychotherapy and Medical Psychology, University of Wurzburg, Germany
    Int J Rehabil Res 26:85-91. 2003
    ..In contrast to other sensitivity-to-change indices, such an approach takes the different reliabilities of competitive assessment instruments explicitly into account...
  86. ncbi request reprint Crystal structure of the C-terminal peptidoglycan-binding domain of human peptidoglycan recognition protein Ialpha
    Rongjin Guan
    Center for Advanced Research in Biotechnology, W M Keck Laboratory for Structural Biology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    J Biol Chem 279:31873-82. 2004
    ..We further demonstrate sodium-dependent dimerization of PGRP-Ialpha in solution, suggesting a possible mechanism for modulating PGRP activity through the formation of multivalent adducts...
  87. ncbi request reprint Assessing reproducibility for interval data in health-related quality of life questionnaires: which coefficient should be used?
    Peter Schuck
    Rehabilitation Sciences Section, Institute of Psychotherapy and Medical Psychology, University of Wurzburg, Wurzburg, Germany
    Qual Life Res 13:571-86. 2004
    ..Given this assumption, an ICC computed from a one-way ANOVA seems to be the best choice for this purpose. Otherwise, Lin's concordance correlation coefficient is recommended as identity measure...
  88. pmc Characterization of DC-SIGN/R interaction with human immunodeficiency virus type 1 gp120 and ICAM molecules favors the receptor's role as an antigen-capturing rather than an adhesion receptor
    Greg A Snyder
    Laboratory of Immunogenetics, Structural Immunology Section, NIAID, NIH, Twinbrook II, 12441 Parklawn Dr, Rockville, MD 20852, USA
    J Virol 79:4589-98. 2005
    ..Our work contradicts the function of DC-SIGN as a Trojan horse to facilitate HIV-1 infection; rather, it supports the function of DC-SIGN/R (a designation referring to both DC-SIGN and DC-SIGNR) as an antigen-capturing receptor...
  89. doi request reprint Apolipoprotein C-II amyloid fibrils assemble via a reversible pathway that includes fibril breaking and rejoining
    Katrina J Binger
    Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, 30 Flemington Road, Parkville, Victoria 3010, Australia
    J Mol Biol 376:1116-29. 2008
    ..These studies establish the importance of breaking and rejoining in amyloid fibril formation and identify prospective new therapeutic targets in the assembly pathway...
  90. ncbi request reprint Conformational restriction blocks glutamate receptor desensitization
    Matthew C Weston
    Department of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA
    Nat Struct Mol Biol 13:1120-7. 2006
    ....
  91. ncbi request reprint Superantigen natural affinity maturation revealed by the crystal structure of staphylococcal enterotoxin G and its binding to T-cell receptor Vbeta8.2
    Marisa M Fernández
    Instituto de Estudios de la Inmunidad Humoral, Laboratorio de Inmunología Estructural, CONICET, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Buenos Aires, Argentina
    Proteins 68:389-402. 2007
    ..2 binding site without affecting the binding to MHC-II. This results in a SAg with improved binding to its natural ligands, which may confer a possible evolutionary advantage for bacterial strains expressing SEG...