Research Topics
Species | P SchuckSummaryAffiliation: National Institutes of Health Country: USA Publications
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Detail Information
Publications
Size-distribution analysis of proteins by analytical ultracentrifugation: strategies and application to model systemsPeter Schuck
Division of Bioengineering and Physical Science, Office of Research Services, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, 20892, USA
Biophys J 82:1096-111. 2002..Although c(s) is computationally the most complex, it has the potential for the highest resolution and sensitivity of the methods described...
Regulation of ASAP1 by phospholipids is dependent on the interface between the PH and Arf GAP domainsMagnus M Che
Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, United States
Cell Signal 17:1276-88. 2005....- On the analysis of sedimentation velocity in the study of protein complexesPatrick H Brown
Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892 5766, USA
Eur Biophys J 38:1079-99. 2009..We describe how these effects can be computationally accounted for in SEDFIT and SEDPHAT... - Kinetics of ligand binding to receptor immobilized in a polymer matrix, as detected with an evanescent wave biosensor. I. A computer simulation of the influence of mass transportP Schuck
Laboratory of Biochemical Pharmacology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institute of Health, Bethesda, Maryland 20892, USA
Biophys J 70:1230-49. 1996..The use of a thinner polymer matrix could significantly increase the range of detectable rate constants... - On the analysis of protein self-association by sedimentation velocity analytical ultracentrifugationPeter Schuck
Protein Biophysics Resource, Division of Bioengineering and Physical Science, ORS, OD, National Institutes of Health, Bethesda, MD 20892, USA
Anal Biochem 320:104-24. 2003..Further, under suitable conditions, fractions of incompetent material that are not part of the reversible equilibrium can be detected... - A model for sedimentation in inhomogeneous media. I. Dynamic density gradients from sedimenting co-solutesPeter Schuck
Division of Bioengineering and Physical Science, ORS, OD, National Institutes of Health, Building 13, Room 3N17, 13 South Drive, Bethesda, MD 20892 5766, USA
Biophys Chem 108:187-200. 2004..General features of protein sedimentation in dynamic density gradients are described... - Analysis of transport experiments using pseudo-absorbance dataS R Kar
Molecular Interactions Resource, National Institutes of Health, Bethesda, Maryland 20892, USA
Anal Biochem 285:135-42. 2000..It is shown that pseudo-absorbance analysis can increase the capacity of a sedimentation velocity experiment in ultracentrifugation, and, in general, can considerably simplify the requirements of optical design... - Determination of the sedimentation coefficient distribution by least-squares boundary modelingP Schuck
Molecular Interactions Resource, Bioengineering and Physical Science Program, ORS, National Institutes of Health, Bethesda, Maryland 20892, USA
Biopolymers 54:328-41. 2000..Because the method does not require a high frequency of scans, it can also be easily used in experiments with the absorbance optical scanning system. Published 2000 John Wiley & Sons, Inc... - A model for sedimentation in inhomogeneous media. II. Compressibility of aqueous and organic solventsPeter Schuck
Division of Bioengineering and Physical Science, ORS, OD, National Institutes of Health, Building 13, Rm 3N17, 13 South Drive, Bethesda, MD 20892 5766, USA
Biophys Chem 108:201-14. 2004..The consideration of solvent compressibility is highly relevant not only when using organic solvents, but also in aqueous solvents when precise sedimentation coefficients are needed, for example, for hydrodynamic modeling... - Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and lamm equation modelingP Schuck
Molecular Interactions Resource, Bioengineering and Physical Science Program, ORS, National Institutes of Health, Bethesda, Maryland 20892, USA
Biophys J 78:1606-19. 2000.... - Direct sedimentation analysis of interference optical data in analytical ultracentrifugationP Schuck
Molecular Interactions Resource, Bioengineering and Physical Science Program, ORS, National Institutes of Health, Bethesda, Maryland 20892, USA
Biophys J 76:2288-96. 1999..They can be extracted from data acquired during the approach to equilibrium, and can be used to increase the reliability of the results obtained from a sedimentation equilibrium analysis... - Kinetic analysis of biosensor data: elementary tests for self-consistencyP Schuck
Section of Physical Biochemistry, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Trends Biochem Sci 21:458-60. 1996..A search of the recent literature reveals that many published results fail these tests qualitatively... - Use of surface plasmon resonance to probe the equilibrium and dynamic aspects of interactions between biological macromoleculesP Schuck
Section of Physical Biochemistry, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Annu Rev Biophys Biomol Struct 26:541-66. 1997..Described here is the influence of these artifacts on the measured binding kinetics and equilibria, together with suggested control experiments... - Reliable determination of binding affinity and kinetics using surface plasmon resonance biosensorsP Schuck
Biomedical Engineering and Instrumentation Program, National Institutes of Health, Bethesda, MD 20892 5766, USA
Curr Opin Biotechnol 8:498-502. 1997..Improved analytical approaches include the application of global analysis and analytical corrections for the influence of mass transport... - Determination of sedimentation coefficients for small peptidesP Schuck
Biomedical Engineering and Instrumentation Program, ORS, National Institutes of Health, Bethesda, Maryland 20892, USA
Biophys J 74:466-74. 1998.... - Rapid determination of molar mass in modified Archibald experiments using direct fitting of the Lamm equationP Schuck
Molecular Interactions Resource, BEPS, OD, National Institutes of Health, Bethesda, Maryland 20892, USA
Anal Biochem 259:48-53. 1998.... - Sedimentation analysis of noninteracting and self-associating solutes using numerical solutions to the Lamm equationP Schuck
Bioengineering and Physical Science Program, National Institutes of Health, Bethesda, Maryland 20892, USA
Biophys J 75:1503-12. 1998..In addition, in the case of a protein in rapid monomer-dimer equilibrium, this configuration was found to reveal the most precise estimate of the association constant... - Determination of binding constants by equilibrium titration with circulating sample in a surface plasmon resonance biosensorP Schuck
Molecular Interactions Resource, Bioengineering and Physical Science Program, ORS, National Institutes of Health, Bethesda, Maryland 20892, USA
Anal Biochem 265:79-91. 1998..The rapid injection of high concentrations of competitor allows the observation of fast dissociation processes under conditions minimizing rebinding... - Rotavirus nonstructural protein NSP2 self-assembles into octamers that undergo ligand-induced conformational changesP Schuck
Division of Bioengineering and Physical Science, ORS, OD, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 276:9679-87. 2001..Chen, D., and Patton, J. T. (1999) J. Virol. 73, 9934-9943) of its function as a molecular motor involved in the packaging of viral mRNA... - Bayesian analysis of heterogeneity in the distribution of binding properties of immobilized surface sitesInna I Gorshkova
Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892, USA
Langmuir 24:11577-86. 2008..The distributions obtained are highly reproducible. Immobilization conditions and the total surface density of immobilized sites can have a substantial impact on the functional distribution of the binding sites... - Sedimentation velocity analysis of heterogeneous protein-protein interactions: Lamm equation modeling and sedimentation coefficient distributions c(s)Julie Dam
Center for Advanced Research in Biotechnology, W. M. Keck Laboratory for Structural Biology, University of Maryland Biotechnology Institute, Rockville, Maryland, USA
Biophys J 89:619-34. 2005..The integration of the c(s) peaks can lead to isotherms of species populations or s-value of the reaction boundary, respectively, which can be used for the determination of the equilibrium binding constant... - Sedimentation velocity analysis of heterogeneous protein-protein interactions: sedimentation coefficient distributions c(s) and asymptotic boundary profiles from Gilbert-Jenkins theoryJulie Dam
Center for Advanced Research in Biotechnology, W. M. Keck Laboratory for Structural Biology, University of Maryland Biotechnology Institute, Rockville, Maryland, USA
Biophys J 89:651-66. 2005..This can significantly improve the estimates for the determination of binding constants and hydrodynamic parameters of the complexes... - Macromolecular size-and-shape distributions by sedimentation velocity analytical ultracentrifugationPatrick H Brown
Protein Biophysics Resource, Division of Bioengineering and Physical Science, ORS, Office of the Director, National Institutes of Health, Bethesda, Maryland 20892, USA
Biophys J 90:4651-61. 2006..We show that this can be used to determine average molar masses of macromolecules and characterize macromolecular distributions, without the approximation of any scaling relationship between hydrodynamic and thermodynamic parameters... - Calcium-sensitive interaction between calmodulin and modified forms of rat brain neurogranin/RC3K P Huang
Section on Metabolic Regulation, National Institutes of Health, Bethesda, MD 20892 4510, USA
Biochemistry 39:7291-9. 2000..These results indicate that modification of NG to form intramolecular disulfides outside the IQ domain provides an alternative mechanism for regulation of its binding affinity to CaM... - Adaptation of a surface plasmon resonance biosensor with microfluidics for use with small sample volumes and long contact timesM Abrantes
Molecular Interactions Resource, Division of Bioengineering and Physical Science, ORS, OD, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Anal Chem 73:2828-35. 2001..This can translate to improved detection limits for slow reactions and can facilitate kinetic and thermodynamic binding studies... - Novel chimpanzee/human monoclonal antibodies that neutralize anthrax lethal factor, and evidence for possible synergy with anti-protective antigen antibodyZhaochun Chen
Laboratory of Infectious Diseases1 and Laboratory of Bacterial Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Infect Immun 77:3902-8. 2009..Although two unique MAbs could protect against anthrax when used alone, even more efficient and broader protection should be gained by combining them with anti-PA MAbs... - Self-association and ligand-induced conformational changes of iron regulatory proteins 1 and 2Emine Yikilmaz
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 5766, USA
Biochemistry 44:8470-8. 2005..The dynamic change of the IRP between different oligomeric and conformational states induced by interaction with IRE may play a role in the iron regulatory functions of IRPs... - Chimpanzee/human mAbs to vaccinia virus B5 protein neutralize vaccinia and smallpox viruses and protect mice against vaccinia virusZhaochun Chen
Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Office of Research Services, Office of the Director, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 103:1882-7. 2006..These chimpanzee/human anti-B5 mAbs may be useful in the prevention and treatment of vaccinia virus-induced complications of vaccination against smallpox and may also be effective in the immunoprophylaxis and immunotherapy of smallpox... - Sedimentation equilibrium analysis of protein interactions with global implicit mass conservation constraints and systematic noise decompositionJennifer Vistica
Protein Biophysics Resource, Division of Bioengineering and Physical Science, ORS, OD, National Institutes of Health, Bethesda, MD 20892, USA
Anal Biochem 326:234-56. 2004..The properties of these tools are studied with theoretical and experimental data sets... - Using prior knowledge in the determination of macromolecular size-distributions by analytical ultracentrifugationPatrick H Brown
Protein Biophysics Resource, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892, USA
Biomacromolecules 8:2011-24. 2007..Further, the use of multiple alternative prior information allows us to probe the range of possible interpretations consistent with the data... - Alanine-scanning mutations in domain 4 of anthrax toxin protective antigen reveal residues important for binding to the cellular receptor and to a neutralizing monoclonal antibodyM J Rosovitz
Microbial Pathogenesis Section, NIAID, National Institutes of Health, Bethesda, Maryland 20892 4350, USA
J Biol Chem 278:30936-44. 2003..The K684A and L685A variants exhibited wild type levels of toxicity in cell culture assays; the L687A and Y688A variants were reduced only 1.5- and 5-fold, respectively... 
Characterizing protein-protein interactions by sedimentation velocity analytical ultracentrifugationPatrick H Brown
National Institutes of Health, Bethesda, Maryland, USA
Curr Protoc Immunol . 2008..Concepts for the experimental design are discussed, and a detailed step-by-step protocol guiding the reader through the experiment and the data analysis is available as an Internet resource...- Measuring protein interactions by optical biosensorsP Schuck
National Institutes of Health, Bethesda, Maryland, USA
Curr Protoc Protein Sci . 2001..Emphasis is given to the description of simple and robust approaches that will provide reliable results. How to avoid the most commonly encountered problems and artifacts is also discussed... - Combined affinity and rate constant distributions of ligand populations from experimental surface binding kinetics and equilibriaJuraj Svitel
Division of Bioengineering and Physical Science, ORS, Office of the Director, National Institutes of Health, Bethesda, Maryland 20892, USA
Biophys J 84:4062-77. 2003..The distribution analysis can provide a rational interpretation of complex experimental surface binding kinetics, and provides an analytical tool for probing the homogeneity of the populations of immobilized protein... - Measuring protein-protein interactions by equilibrium sedimentationAndrea Balbo
National Institutes of Health, Bethesda, Maryland, USA
Curr Protoc Immunol . 2007..A detailed protocol guiding the investigator through the experimental steps and the data analysis is available as an internet resource... - Hydrodynamics of nanoscopic tubulin rings in dilute solutionsHacene Boukari
Laboratory of Integrative and Medical Biophysics, NICHD, Bethesda, Maryland 20892, USA
Phys Rev Lett 93:098106. 2004..We use the high-precision ratio of the sedimentation coefficients and that of the translational diffusion coefficients to validate theories for calculating the hydrodynamic properties of supramolecular structures... - Measuring protein interactions by optical biosensorsPeter Schuck
National Institutes of Health, Bethesda, Maryland, USA
Curr Protoc Cell Biol . 2004..This unit provides a thorough, well-illustrated discussion of the principles of optical biosensors, experimental design, ligand immobilization, experimental data analysis, and common obstacles and possible solutions... - Accounting for solvent signal offsets in the analysis of interferometric sedimentation velocity dataHuaying Zhao
Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, NIBIB, National Institutes of Health, Bethesda, Maryland, USA
Macromol Biosci 10:736-45. 2010..We demonstrate how this can restore the SV analysis to yield a high quality fit of the data and to provide correct macromolecular sedimentation parameters... - A bayesian approach for quantifying trace amounts of antibody aggregates by sedimentation velocity analytical ultracentrifugationPatrick H Brown
Dynamics of Macromolecular Assembly Section, Laboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892 5766, USA
AAPS J 10:481-93. 2008..Implications for the experimental design of SV-AUC and practical detection limits are discussed... - Single-walled tubulin ring polymersHacene Boukari
Laboratory of Integrative and Medical Biophysics, NICHD, National Institutes of Health, Bethesda, MD 20892, USA
Biopolymers 86:424-36. 2007..Moreover, we show that, by using several techniques, one can eliminate degeneracy to provide better discrimination between model structures... - Humanized monoclonal antibodies derived from chimpanzee Fabs protect against Japanese encephalitis virus in vitro and in vivoAna P Goncalvez
Molecular Viral Biology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
J Virol 82:7009-21. 2008.... - Some statistical properties of differencing schemes for baseline correction of sedimentation velocity dataPeter Schuck
Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA
Anal Biochem 401:280-7. 2010.... - Sedimentation patterns of rapidly reversible protein interactionsPeter Schuck
Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
Biophys J 98:2005-13. 2010..Adding to the existing numerical solutions of the relevant partial differential equations, the effective particle model provides physical insights into the relationships of the parameters that govern sedimentation patterns... - Two interferons alpha influence each other during their interaction with the extracellular domain of human type interferon receptor subunit 2Hana Schmeisser
National Institute of Allergy and Infectious Diseases and National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892, USA
Biochemistry 46:14638-49. 2007..This study contributes to the understanding of the mutual interaction between multiple IFN-alpha subtypes during the competition for binding to the receptor... - On computational approaches for size-and-shape distributions from sedimentation velocity analytical ultracentrifugationPeter Schuck
Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, National Institutes of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA
Eur Biophys J 39:1261-75. 2010..While the 2DSA appears computationally excessively wasteful, key elements also appear to be in conflict with mathematical results. This raises doubts about the correctness of the results from 2DSA analysis... - Characterization of chimpanzee/human monoclonal antibodies to vaccinia virus A33 glycoprotein and its variola virus homolog in vitro and in a vaccinia virus mouse protection modelZhaochun Chen
Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, NIH, 50 South Drive, MSC 8009, Bethesda, MD 20892, USA
J Virol 81:8989-95. 2007.... - Diffusion of the reaction boundary of rapidly interacting macromolecules in sedimentation velocityPeter Schuck
Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
Biophys J 98:2741-51. 2010.... - Grafting of "abbreviated" complementarity-determining regions containing specificity-determining residues essential for ligand contact to engineer a less immunogenic humanized monoclonal antibodyRoberto De Pascalis
Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 169:3076-84. 2002..The final variant of the HuCOL-1, which retains its Ag-binding reactivity and shows significantly lower serum reactivity than that of the parental Ab, can serve as a prototype for the development of a potentially useful clinical reagent... - Modern analytical ultracentrifugation in protein science: a tutorial reviewJacob Lebowitz
Molecular Interactions Resource, Division of Bioengineering and Physical Science, ORS, OD, National Institutes of Health, Bethesda, Maryland 20892, USA
Protein Sci 11:2067-79. 2002..This is the method of choice for molar mass determinations and the study of self-association and heterogeneous interactions, such as protein-protein, protein-nucleic acid, and protein-small molecule binding... - Binding specificity of multiprotein signaling complexes is determined by both cooperative interactions and affinity preferencesJon C D Houtman
Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Biochemistry 43:4170-8. 2004..These studies provide quantitative information on how different binding parameters can determine in vivo binding site specificity observed for multiprotein signaling complexes... - Efficient neutralization of anthrax toxin by chimpanzee monoclonal antibodies against protective antigenZhaochun Chen
Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
J Infect Dis 193:625-33. 2006..These data suggest that W1 and W2 chimpanzee monoclonal antibodies may serve as PA entry inhibitors for use in the emergency prophylaxis against and treatment of anthrax... - Biochemical and biological characterization of a dodecameric CD4-Ig fusion protein: implications for therapeutic and vaccine strategiesJames Arthos
Laboratory of Immunoregulation, NIAID, and the Molecular Interactions Resource Division of Bioengineering and Physical Science, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 277:11456-64. 2002..This protein does not enhance viral replication at suboptimal concentrations. These observations may aid in the design of new therapeutics and vaccines... - Inhibition of hemostasis by a high affinity biogenic amine-binding protein from the saliva of a blood-feeding insectJohn F Andersen
Laboratory of Malaria and Vector Research, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 278:4611-7. 2003..Molecular modeling of ABP suggests that ligand binding is mediated by interaction with the side chains of aromatic amino acids and charged residues that line the binding pocket... - Autoinhibition of Arf GTPase-activating protein activity by the BAR domain in ASAP1Xiaoying Jian
Laboratory of Cellular and Molecular Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 284:1652-63. 2009..Analysis of single turnover kinetics revealed a transition state intermediate that was affected by the BAR domain. We conclude that BAR domains can affect enzymatic activity through intraprotein interactions... - Energetics of glutamate receptor ligand binding domain dimer assembly are modulated by allosteric ionsCharu Chaudhry
Laboratory of Cellular and Molecular Neurophysiology, Porter Neuroscience Research Center, National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 106:12329-34. 2009.... - Elucidating kinetic and thermodynamic constants for interaction of G protein subunits and receptors by surface plasmon resonance spectroscopyR Victor Rebois
Laboratory of Molecular and Cellular Neurobiology and Biology, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
Methods Enzymol 344:15-42. 2002 - Studying multiprotein complexes by multisignal sedimentation velocity analytical ultracentrifugationAndrea Balbo
Protein Biophysics Resource, Division of Bioengineering and Physical Science, Office of Research Services, Office of the Director, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 102:81-6. 2005.... - Crystal structure of an NK cell immunoglobulin-like receptor in complex with its class I MHC ligandJ C Boyington
Structural Biology Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA
Nature 405:537-43. 2000..A second KIR/HLA interface produced an ordered receptor-ligand aggregation in the crystal which may resemble receptor clustering during immune synapse formation... - Oligomeric structure of virion-associated and soluble forms of the simian immunodeficiency virus envelope protein in the prefusion activated conformationR J Center
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, and Division of Bioengineering and Physical Science, Office of Research Services, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 98:14877-82. 2001..Furthermore, a triangular or tri-lobed morphology was clearly visualized in a subset of the trimers... - Dissection of merozoite surface protein 3, a representative of a family of Plasmodium falciparum surface proteins, reveals an oligomeric and highly elongated moleculeBrandt R Burgess
Structural Biology Section, Laboratory of Immunogenetics, NIAID, National Institutes of Health, Rockville, Maryland 20852, USA
J Biol Chem 280:37236-45. 2005.... - Eukaryotic RNases H1 act processively by interactions through the duplex RNA-binding domainSergei A Gaidamakov
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development Bethesda, MD 20892, USA
Nucleic Acids Res 33:2166-75. 2005..In mitochondria, where RNase H1 is essential for DNA formation during embryogenesis, long hybrids may be involved in DNA replication... - Mass spectrometry after capture and small-volume elution of analyte from a surface plasmon resonance biosensorJohn J Gilligan
Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
Anal Chem 74:2041-7. 2002..Examples are presented for the specific detection and recovery of a protein from a complex mixture of cytosolic proteins... - Probing the functional heterogeneity of surface binding sites by analysis of experimental binding traces and the effect of mass transport limitationJuraj Svitel
National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
Biophys J 92:1742-58. 2007..In the latter regime, we report experimental evidence for the formation of gradients within the sensing volume of the evanescent field biosensor used... - The role of mass transport limitation and surface heterogeneity in the biophysical characterization of macromolecular binding processes by SPR biosensingPeter Schuck
Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering NIBIB, National Institutes of Health, Bethesda, MD, USA
Methods Mol Biol 627:15-54. 2010..The effect of these factors on the observed binding kinetics, and strategies to account for them are reviewed, both in the context of mathematical data analysis, as well as the design of the experiments and controls... - Direct interaction of the mouse cytomegalovirus m152/gp40 immunoevasin with RAE-1 isoformsLi Zhi
Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 1892, USA
Biochemistry 49:2443-53. 2010..Molecular modeling of the different RAE-1 isoforms suggests a potential site for the m152 interaction... - Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgGJohnson Agniswamy
Structural Immunology Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12441 Parklawn Drive, Rockville, Maryland 20852, USA
Structure 14:225-35. 2006..A tunnel observed at the dimer interface constitutes a target for designing potential Mac-1-specific antimicrobial agents. The structures also offer insight into the functional difference between Mac-1 and Mac-2... - Surface plasmon resonance-based competition assay to assess the sera reactivity of variants of humanized antibodiesNoreen R Gonzales
Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 10 Center Drive, Room 8B09, Bethesda, MD 20892, USA
J Immunol Methods 268:197-210. 2002..This may facilitate the rapid screening, for the presence of anti-VR Abs, of the sera of patients undergoing clinical trials... - Analysis of a temperature-sensitive mutant rotavirus indicates that NSP2 octamers are the functional form of the proteinZenobia F Taraporewala
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Virol 76:7082-93. 2002..The propensity of tsE NSP2 to form large aggregates provides a possible explanation for the inability of the protein to support packaging and/or replication in the infected cell at the nonpermissive temperature... - Extended polypeptide linkers establish the spatial architecture of a pyruvate dehydrogenase multienzyme complexJeffrey S Lengyel
Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Structure 16:93-103. 2008.... - Cellular expression and crystal structure of the murine cytomegalovirus major histocompatibility complex class I-like glycoprotein, m153Janet Mans
Molecular Biology Section, Laboratory of Immunology, NIAID, National Institutes of Health, Bethesda, Maryland 20892 1892, USA
J Biol Chem 282:35247-58. 2007..The structure provides a useful framework for comparative analysis of the divergent members of the m145 family... - In vitro affinity maturation of a specificity-determining region-grafted humanized anticarcinoma antibody: isolation and characterization of minimally immunogenic high-affinity variantsRoberto De Pascalis
Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
Clin Cancer Res 9:5521-31. 2003..These results demonstrate that phage display can be used to isolate variants of an Ab that are potentially less immunogenic in patients than the parental Ab from which they are derived... - Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1Jon C D Houtman
Laboratory of Cellular and Molecular Biology, National Cancer Institute, US National Institutes of Health, Bethesda, Maryland 20892, USA
Nat Struct Mol Biol 13:798-805. 2006..These data suggest that multipoint binding of distal adaptor proteins mediates the formation of oligomeric signaling clusters vital for intracellular signaling... - Stability of ligand-binding domain dimer assembly controls kainate receptor desensitizationCharu Chaudhry
Laboratory of Cellular and Molecular Neurophysiology, Porter Neuroscience Research Center, NICHD, NIH, DHHS, Bethesda, MD, USA
EMBO J 28:1518-30. 2009..Our results show how neurotransmitter receptors with similar structures and gating mechanisms can exhibit strikingly different functional properties... - Quantifying the energetics of cooperativity in a ternary protein complexPeter S Andersen
Institute of Medical Microbiology and Immunology, University of Copenhagen, Blegdamsvej 3C, DK-2200 Copenhagen N, Denmark
Biochemistry 41:5177-84. 2002..Our experimental and theoretical analysis may be applicable to other systems involving cooperativity... - Cooperative behavior of Escherichia coli cell-division protein FtsZ assembly involves the preferential cyclization of long single-stranded fibrilsJose Manuel Gonzalez
Centro de Investigaciones Biológicas and Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Cientificas, 28040 Madrid, Spain
Proc Natl Acad Sci U S A 102:1895-900. 2005..The proposed model is in accord with the results obtained from our experimental observations... - Influence of temperature on the conformation of canine plasminogen: an analytical ultracentrifugation and dynamic light scattering studyJack A Kornblatt
Enzyme Research Group, Department of Biology, Concordia University, 7141 Sherbrooke Ouest, Montreal, Quebec, Canada
Biochemistry 44:13122-31. 2005.... - Calculating sedimentation coefficient distributions by direct modeling of sedimentation velocity concentration profilesJulie Dam
Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
Methods Enzymol 384:185-212. 2004 - Studying multisite binary and ternary protein interactions by global analysis of isothermal titration calorimetry data in SEDPHAT: application to adaptor protein complexes in cell signalingJon C D Houtman
Department of Microbiology, University of Iowa, Iowa 52242, USA
Protein Sci 16:30-42. 2007.... - Reversible and fast association equilibria of a molecular chaperone, gp57A, of bacteriophage T4Said A Ali
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan
Biophys J 85:2606-18. 2003..Trimer or hexamer is likely the functional oligomeric state of gp57A... - Bacteriorhodopsin/amphipol complexes: structural and functional propertiesYann Gohon
Laboratoire de Physico Chimie Moléculaire des Membranes Biologiques, Centre National de la Recherche Scientifique, and Université Paris 7, Institut de Biologie Physico Chimique, Paris, France
Biophys J 94:3523-37. 2008..In the absence of free APol, BR/APol particles can autoassociate into small or large ordered fibrils... - Variable MHC class I engagement by Ly49 natural killer cell receptors demonstrated by the crystal structure of Ly49C bound to H-2K(b)Julie Dam
Center for Advanced Research in Biotechnology, W M Keck Laboratory for Structural Biology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA
Nat Immunol 4:1213-22. 2003..We propose a dynamic model for Ly49-MHC class I interactions involving conformational changes in the receptor, whereby variations in Ly49 dimerization mediate different MHC-binding modes... - Targeted lysis of HIV-infected cells by natural killer cells armed and triggered by a recombinant immunoglobulin fusion protein: implications for immunotherapyNeil Gupta
Laboratory of Immunoregulation, NIAID, NIH Bldg. #10 6A08, 9000 Rockville Pike, Bethesda MD 20892, USA
Virology 332:491-7. 2005..NK cells pre-armed in this manner retain the capacity to kill targets over an extended period of time. This strategy may have application to other disease states including various viral infections and cancers... - A mechanism for assembly of complexes of vitronectin and plasminogen activator inhibitor-1 from sedimentation velocity analysisKenneth H Minor
Department of Biochemistry, Cellular, and Molecular Biology and the Center of Excellence in Structural Biology, University of Tennessee, Knoxville, Tennessee 37996, USA
J Biol Chem 280:28711-20. 2005..This 2:1 PAI-1-vitronectin complex, with a sedimentation coefficient of 6.5 S, is the key intermediate for the assembly of higher order complexes... - Differences in the binding capacity of human apolipoprotein E3 and E4 to size-fractionated lipid emulsionsMatthew A Perugini
Department of Biochemistry and Molecular Biology, The University of Melbourne, Parkville, Vic, Australia
Eur J Biochem 269:5939-49. 2002..Implications for the role of apoE4 in blood lipid transport and disease are discussed... - The 'smallest real difference' as a measure of sensitivity to change: a critical analysisPeter Schuck
Institute of Psychotherapy and Medical Psychology, University of Wurzburg, Germany
Int J Rehabil Res 26:85-91. 2003..In contrast to other sensitivity-to-change indices, such an approach takes the different reliabilities of competitive assessment instruments explicitly into account... - Crystal structure of the C-terminal peptidoglycan-binding domain of human peptidoglycan recognition protein IalphaRongjin Guan
Center for Advanced Research in Biotechnology, W M Keck Laboratory for Structural Biology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
J Biol Chem 279:31873-82. 2004..We further demonstrate sodium-dependent dimerization of PGRP-Ialpha in solution, suggesting a possible mechanism for modulating PGRP activity through the formation of multivalent adducts... - Assessing reproducibility for interval data in health-related quality of life questionnaires: which coefficient should be used?Peter Schuck
Rehabilitation Sciences Section, Institute of Psychotherapy and Medical Psychology, University of Wurzburg, Wurzburg, Germany
Qual Life Res 13:571-86. 2004..Given this assumption, an ICC computed from a one-way ANOVA seems to be the best choice for this purpose. Otherwise, Lin's concordance correlation coefficient is recommended as identity measure... - Characterization of DC-SIGN/R interaction with human immunodeficiency virus type 1 gp120 and ICAM molecules favors the receptor's role as an antigen-capturing rather than an adhesion receptorGreg A Snyder
Laboratory of Immunogenetics, Structural Immunology Section, NIAID, NIH, Twinbrook II, 12441 Parklawn Dr, Rockville, MD 20852, USA
J Virol 79:4589-98. 2005..Our work contradicts the function of DC-SIGN as a Trojan horse to facilitate HIV-1 infection; rather, it supports the function of DC-SIGN/R (a designation referring to both DC-SIGN and DC-SIGNR) as an antigen-capturing receptor... - Apolipoprotein C-II amyloid fibrils assemble via a reversible pathway that includes fibril breaking and rejoiningKatrina J Binger
Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, 30 Flemington Road, Parkville, Victoria 3010, Australia
J Mol Biol 376:1116-29. 2008..These studies establish the importance of breaking and rejoining in amyloid fibril formation and identify prospective new therapeutic targets in the assembly pathway... - Conformational restriction blocks glutamate receptor desensitizationMatthew C Weston
Department of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA
Nat Struct Mol Biol 13:1120-7. 2006.... - Superantigen natural affinity maturation revealed by the crystal structure of staphylococcal enterotoxin G and its binding to T-cell receptor Vbeta8.2Marisa M Fernández
Instituto de Estudios de la Inmunidad Humoral, Laboratorio de Inmunología Estructural, CONICET, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Buenos Aires, Argentina
Proteins 68:389-402. 2007..2 binding site without affecting the binding to MHC-II. This results in a SAg with improved binding to its natural ligands, which may confer a possible evolutionary advantage for bacterial strains expressing SEG...