A N Schechter

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc NO solutions?
    Alan N Schechter
    Laboratory of Chemical Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, Maryland 20892, USA
    J Clin Invest 109:1149-51. 2002
  2. ncbi request reprint Inhaled nebulized nitrite is a hypoxia-sensitive NO-dependent selective pulmonary vasodilator
    Christian J Hunter
    Critical Care Medicine Department, Clinical Center, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, Maryland 20892 1662, USA
    Nat Med 10:1122-7. 2004
  3. pmc Hemolysis-associated endothelial dysfunction mediated by accelerated NO inactivation by decompartmentalized oxyhemoglobin
    Peter C Minneci
    Critical Care Medicine Department, NIH, Bethesda, Maryland 20892, USA
    J Clin Invest 115:3409-17. 2005
  4. ncbi request reprint Hemoglobin and the paracrine and endocrine functions of nitric oxide
    Alan N Schechter
    National Institute of Diabetes and Digestive and Kidney Diseases, and the Critical Care Medicine Department, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892 1822, USA
    N Engl J Med 348:1483-5. 2003
  5. pmc JAK-STAT and AKT pathway-coupled genes in erythroid progenitor cells through ontogeny
    Vladan P Cokic
    Laboratory of Experimental Hematology, Institute for Medical Research, University of Belgrade, Belgrade, 11129, Serbia
    J Transl Med 10:116. 2012
  6. pmc Globin gene expression in correlation with G protein-related genes during erythroid differentiation
    Vladan P Cokic
    Laboratory of Experimental Hematology, Institute for Medical Research, University of Belgrade, Dr, Subotića 4, 11129, Belgrade, Serbia
    BMC Genomics 14:116. 2013
  7. pmc Translational medicine--doing it backwards
    Robert B Nussenblatt
    Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Transl Med 8:12. 2010
  8. pmc Hemoglobin research and the origins of molecular medicine
    Alan N Schechter
    Molecular Medicine Branch, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    Blood 112:3927-38. 2008
  9. ncbi request reprint Why is revitalizing clinical research so important, yet so difficult?
    Alan N Schechter
    Laboratory of Chemical Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892 1822, USA
    Perspect Biol Med 47:476-86. 2004
  10. ncbi request reprint Prematurity in gene therapy
    Alan N Schechter
    Laboratory of Chemical Biology NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Ther 5:217-9. 2002

Collaborators

Detail Information

Publications64

  1. pmc NO solutions?
    Alan N Schechter
    Laboratory of Chemical Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, Maryland 20892, USA
    J Clin Invest 109:1149-51. 2002
  2. ncbi request reprint Inhaled nebulized nitrite is a hypoxia-sensitive NO-dependent selective pulmonary vasodilator
    Christian J Hunter
    Critical Care Medicine Department, Clinical Center, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, Maryland 20892 1662, USA
    Nat Med 10:1122-7. 2004
    ....
  3. pmc Hemolysis-associated endothelial dysfunction mediated by accelerated NO inactivation by decompartmentalized oxyhemoglobin
    Peter C Minneci
    Critical Care Medicine Department, NIH, Bethesda, Maryland 20892, USA
    J Clin Invest 115:3409-17. 2005
    ....
  4. ncbi request reprint Hemoglobin and the paracrine and endocrine functions of nitric oxide
    Alan N Schechter
    National Institute of Diabetes and Digestive and Kidney Diseases, and the Critical Care Medicine Department, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892 1822, USA
    N Engl J Med 348:1483-5. 2003
  5. pmc JAK-STAT and AKT pathway-coupled genes in erythroid progenitor cells through ontogeny
    Vladan P Cokic
    Laboratory of Experimental Hematology, Institute for Medical Research, University of Belgrade, Belgrade, 11129, Serbia
    J Transl Med 10:116. 2012
    ....
  6. pmc Globin gene expression in correlation with G protein-related genes during erythroid differentiation
    Vladan P Cokic
    Laboratory of Experimental Hematology, Institute for Medical Research, University of Belgrade, Dr, Subotića 4, 11129, Belgrade, Serbia
    BMC Genomics 14:116. 2013
    ..By analyzing erythroid progenitors, derived from hematopoietic cells through in vitro ontogeny, our study intends to determine early markers and signaling pathways of globin gene regulation and their relation to GPCR expression...
  7. pmc Translational medicine--doing it backwards
    Robert B Nussenblatt
    Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Transl Med 8:12. 2010
    ..Its mission is science in pursuit of fundamental knowledge about the nature and behavior of living systems and the application of that knowledge to extend healthy life and reduce the burdens of illness and disability 1...
  8. pmc Hemoglobin research and the origins of molecular medicine
    Alan N Schechter
    Molecular Medicine Branch, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    Blood 112:3927-38. 2008
    ..It is likely that current research will also have significant clinical implications, as well as lessons for other aspects of molecular medicine, the origin of which can be largely traced to this research tradition...
  9. ncbi request reprint Why is revitalizing clinical research so important, yet so difficult?
    Alan N Schechter
    Laboratory of Chemical Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892 1822, USA
    Perspect Biol Med 47:476-86. 2004
    ..Discussion of alternate conceptual frameworks for biomedical research should help lead to changes in funding and organizational structures that might finally revitalize clinical research...
  10. ncbi request reprint Prematurity in gene therapy
    Alan N Schechter
    Laboratory of Chemical Biology NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Ther 5:217-9. 2002
  11. pmc Inhaled nitric oxide augments nitric oxide transport on sickle cell hemoglobin without affecting oxygen affinity
    M T Gladwin
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Invest 104:937-45. 1999
    ..The levels of nitrosylated hemoglobin are too low to affect overall hemoglobin oxygen affinity, but augmented NO transport to the microvasculature seems a promising strategy for improving microvascular perfusion...
  12. ncbi request reprint Nitric oxide transport on sickle cell hemoglobin: where does it bind?
    M T Gladwin
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Res 35:175-80. 2001
    ....
  13. pmc Effects of inhaled nitric oxide on regional blood flow are consistent with intravascular nitric oxide delivery
    R O Cannon
    Cardiology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland 20892 1650, USA
    J Clin Invest 108:279-87. 2001
    ..This effect may have application for the treatment of diseases characterized by endothelial dysfunction...
  14. pmc Role of circulating nitrite and S-nitrosohemoglobin in the regulation of regional blood flow in humans
    M T Gladwin
    Critical Care Medicine Department of the Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 97:11482-7. 2000
    ....
  15. ncbi request reprint Nitrite infusion in humans and nonhuman primates: endocrine effects, pharmacokinetics, and tolerance formation
    Andre Dejam
    Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Circulation 116:1821-31. 2007
    ..However, important questions of potency, kinetics, mechanism of action, and possible induction of tolerance remain unanswered...
  16. ncbi request reprint Increasing hemoglobin oxygen saturation levels in sickle trait donor whole blood prevents hemoglobin S polymerization and allows effective white blood cell reduction by filtration
    David F Stroncek
    Department of Tranfusion Medicine, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland, USA
    Transfusion 44:1293-9. 2004
    ..Techniques were investigated to successfully filter Hb AS donor blood by increasing the Hb oxygen saturation with storage bags and conditions suitable for transfusion products...
  17. ncbi request reprint Erythropoietin and hypoxia stimulate erythropoietin receptor and nitric oxide production by endothelial cells
    Bojana B Beleslin-Cokic
    Laboratory of Chemical Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 1822, USA
    Blood 104:2073-80. 2004
    ..This effect of EPO on eNOS may be a physiologically relevant mechanism to counterbalance the hypertensive effects of increased hemoglobin-related NO destruction resulting from hypoxia-induced increased red cell mass...
  18. pmc Enzymatic function of hemoglobin as a nitrite reductase that produces NO under allosteric control
    Zhi Huang
    Vascular Therapeutics Section, Cardiovascular Branch, National Heart, Lung and Blood Institute, and Laboratory of Chemical Biology, National Institute of Diabetes, Digestive and Kidney Diseases, NIH, Bethesda, Maryland 20892 1662, USA
    J Clin Invest 115:2099-107. 2005
    ..These data suggest that the oxygen sensor for hypoxic vasodilation is determined by Hb oxygen saturation and quaternary structure and that the nitrite reductase activity of Hb generates NO gas under allosteric and pH control...
  19. pmc Erythrocytes are the major intravascular storage sites of nitrite in human blood
    Andre Dejam
    Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Disease, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 106:734-9. 2005
    ..These studies suggest a dynamic intravascular NO metabolism in which endothelial NOS-derived NO is stabilized as nitrite, transported by erythrocytes, and consumed during arterial-to-venous transit...
  20. ncbi request reprint Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation
    Kenyatta Cosby
    Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 7B15 Bethesda, Maryland 20892, USA
    Nat Med 9:1498-505. 2003
    ..These results suggest that nitrite represents a major bioavailable pool of NO, and describe a new physiological function for hemoglobin as a nitrite reductase, potentially contributing to hypoxic vasodilation...
  21. ncbi request reprint Pathophysiology of a sickle cell trait mouse model: human alpha(beta)(S) transgenes with one mouse beta-globin allele
    C T Noguchi
    Laboratory of Chemical Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 1822, USA
    Blood Cells Mol Dis 27:971-7. 2001
    ..Analyses of parameters such as P(50), red cell indices, and genetic background are necessary in establishing potential relevance of any mouse model of the sickle cell syndromes...
  22. ncbi request reprint Linderstrom-Lang-Schellman's model for protein stabilization revisited
    Hiroshi Taniuchi
    Laboratory of Chemical Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Protein Pept Sci 5:275-86. 2004
    ....
  23. ncbi request reprint The emerging biology of the nitrite anion
    Mark T Gladwin
    Vascular Medicine Branch, National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, Maryland, USA
    Nat Chem Biol 1:308-14. 2005
    ..A recent two-day symposium at the US National Institutes of Health highlighted recent advances in the understanding of nitrite biochemistry, physiology and therapeutics...
  24. ncbi request reprint Pulmonary hypertension as a risk factor for death in patients with sickle cell disease
    Mark T Gladwin
    Critical Care Medicine Department, Clinical Center, National Institutes of Health, Bethesda, MD 20892 1662, USA
    N Engl J Med 350:886-95. 2004
    ..The prevalence of pulmonary hypertension in adults with sickle cell disease, the mechanism of its development, and its prospective prognostic significance are unknown...
  25. ncbi request reprint Nitric oxide's reactions with hemoglobin: a view through the SNO-storm
    Mark T Gladwin
    Critical Care Medicine Department of the Warren G Magnuson Clinical Center, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Nat Med 9:496-500. 2003
  26. pmc Measurement of nitrite in blood samples using the ferricyanide-based hemoglobin oxidation assay
    Barbora Piknova
    Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Methods Mol Biol 704:39-56. 2011
    ..We also explain how to preserve the original physiological nitrite concentration in nitrite-hostile environments, such as an excess of hemoglobin in blood...
  27. ncbi request reprint Increasing oxygen tension improves filtration of sickle trait donor blood
    Karen M Byrne
    Department of Transfusion Medicine, National Institute of Diabetes and Digestive and Kidney Diseases NIH, Warren G Magnuson Clinical Center, Building 10, Room 1C711, 10 Center Drive MSC 1184, Bethesda, MD 20892 2284, USA
    Br J Haematol 122:678-81. 2003
    ..Bags with added air had increased sO2 levels (from 49 +/- 10% to 76 +/- 6%). Filtration was successful for nine of 10 components with air, and one of 10 without air. Successful filtration of RBC components occurs when sO2 is increased...
  28. ncbi request reprint Nitric oxide donor properties of hydroxyurea in patients with sickle cell disease
    Mark T Gladwin
    Critical Care Medicine Department of the Warren G Magnuson Clinical Center, Bethesda, MD 20892 1662, USA
    Br J Haematol 116:436-44. 2002
    ..The role of NO in the induction of fetal haemoglobin and possible synergy between NO donor therapy and classic cytostatic and differentiating medications should be explored...
  29. ncbi request reprint S-Nitrosohemoglobin is unstable in the reductive erythrocyte environment and lacks O2/NO-linked allosteric function
    Mark T Gladwin
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892 1662, USA
    J Biol Chem 277:27818-28. 2002
    ....
  30. ncbi request reprint Cell-free hemoglobin limits nitric oxide bioavailability in sickle-cell disease
    Christopher D Reiter
    Critical Care Medicine Department of the Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland, USA
    Nat Med 8:1383-9. 2002
    ..Decompartmentalization of hemoglobin and subsequent dioxygenation of nitric oxide may explain the vascular complications shared by acute and chronic hemolytic disorders...
  31. pmc Hemolysis in sickle cell mice causes pulmonary hypertension due to global impairment in nitric oxide bioavailability
    Lewis L Hsu
    Marian Anderson Sickle Cell Center at St Christopher s Hospital for Children, Drexel University College of Medicine, Philadelphia, PA 19134, USA
    Blood 109:3088-98. 2007
    ..Although the time course is shorter than typical pulmonary hypertension in human sickle cell disease, these results demonstrate that hemolytic anemia is sufficient to produce endothelial dysfunction and global dysregulation of NO...
  32. ncbi request reprint Divergent nitric oxide bioavailability in men and women with sickle cell disease
    Mark T Gladwin
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892 1662, USA
    Circulation 107:271-8. 2003
    ..Although reduced endothelial nitric oxide (NO) bioavailability has been demonstrated in arteriosclerotic vascular disease, the integrity of this system in sickle cell disease remains uncertain...
  33. ncbi request reprint Exogenous reference RNA for normalization of real-time quantitative PCR
    Reginald D Smith
    Laboratory of Chemical Biology, NIDDK, NIH, Building 10, Room 9N318, 10 Center Drive, MSC 1822, Bethesda, MD 20892 1822, USA
    Biotechniques 34:88-91. 2003
    ..The use of an exogenous reference such as in vitro transcribed mRNA for the RuBisCO plant gene provides a robust and sample-independent method for the normalization of quantitative PCR data in bacterial and animal cells...
  34. ncbi request reprint Sickle Hb polymerization in RBC components from donors with sickle cell trait prevents effective WBC reduction by filtration
    David F Stroncek
    Department of Transfusion Medicine, Warren G Magnuson Clinical Center, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 1184, USA
    Transfusion 42:1466-72. 2002
    ..RBC components collected from donors with sickle cell trait frequently occlude WBC-reduction filters. In vitro, sickle trait RBCs have the potential for sickle Hb (Hb S) polymerization at low oxygen saturations and high Hb concentrations...
  35. ncbi request reprint Nitric oxide therapy in sickle cell disease
    M T Gladwin
    Critical Care Medicine Department, Warren G. Magnuson Clinical Center, NIH, Bethesda, MD 20892-1662, USA
    Semin Hematol 38:333-42. 2001
    ..While direct evidence for a clinical benefit of NO therapy in sickle cell disease has not been reported, studies are underway to determine if inhaled NO will reduce the substantial morbidity and mortality suffered by these patients...
  36. ncbi request reprint Biological action of nitric oxide donor compounds on platelets from patients with sickle cell disease
    T H Mondoro
    Laboratory of Cellular Hematology and Laboratory of Plasma Derivatives, Center for Biologics Evaluation and Research, Food and Drugs Administration, National Institutes of Health, Bethesda, MD 20892, USA
    Br J Haematol 112:1048-54. 2001
    ..Taken together, these data suggest that the mode of NO delivery to platelets may produce various physiological responses and the optimization of NO delivery may contribute to reducing platelet aggregation in sickle cell disease...
  37. doi request reprint Oxidation of iron-nitrosyl-hemoglobin by dehydroascorbic acid releases nitric oxide to form nitrite in human erythrocytes
    Nathawut Sibmooh
    Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 47:2989-96. 2008
    ..These data provide a physiologically robust mechanism for erythrocytic transport of NO bioactivity allowing for hormone-like properties...
  38. pmc Hydroxyurea induces fetal hemoglobin by the nitric oxide-dependent activation of soluble guanylyl cyclase
    Vladan P Cokic
    Laboratory of Chemical Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
    J Clin Invest 111:231-9. 2003
    ..These data provide strong evidence for an NO-derived mechanism for HbF induction by hydroxyurea and suggest possibilities for therapies based on NO-releasing or -potentiating agents...
  39. ncbi request reprint Emerging role of nitrite in human biology
    Andre Dejam
    Laboratory of Chemical Biology, National Institute of Diabetes, Digestive and Kidney Disease, National Institute of Health, Bethesda, MD 20892, USA
    Blood Cells Mol Dis 32:423-9. 2004
    ..This review focuses on the role of nitrite as a circulating NO donor, its potential as an index of NO synthase (NOS) activity and endothelial function, and discusses potential diagnostic and therapeutic applications...
  40. pmc Biomarker and drug-target discovery using proteomics in a new rat model of sepsis-induced acute renal failure
    M K Holly
    Renal Diagnostics and Therapeutics Unit, NIDDK, Bethesda, Maryland, USA
    Kidney Int 70:496-506. 2006
    ..This model allowed us to use DIGE to find changes in urinary proteins and this approach identified a potential biomarker and drug target - meprin-1-alpha...
  41. pmc Activation of the human epsilon- and beta-globin promoters by SV40 T antigen
    S X Cao
    Laboratory of Chemical Biology, National Institute of Diabetes, and Digestive and Kidney Diseases, Bethesda, MD 20892
    Biochem J 258:769-76. 1989
    ....
  42. pmc Relative role of heme nitrosylation and beta-cysteine 93 nitrosation in the transport and metabolism of nitric oxide by hemoglobin in the human circulation
    M T Gladwin
    Critical Care Medicine Department of the Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 97:9943-8. 2000
    ....
  43. ncbi request reprint A study of the influence of the hydrophobic core residues of yeast iso-2-cytochrome c on phosphate binding: a probe of the hydrophobic core-surface charge interactions
    H Taniuchi
    Laboratory of Chemical Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Protein Chem 20:203-15. 2001
    ..Such an explanation would be in accord with the observed difference in thermal stability between iso-2 and horse cytochromes c...
  44. pmc The reaction of cell-free oxyhemoglobin with nitrite under physiologically relevant conditions: Implications for nitrite-based therapies
    Barbora Piknova
    National Institutes of Health, NIH, NIDDK, Molecular Medicine Branch, Bethesda, MD 20892, USA
    Nitric Oxide 20:88-94. 2009
    ..Our findings suggest that pharmacological doses of nitrite are unlikely to cause free radical or ferrylhemoglobin formation in plasma originating from the reaction of nitrite with cell-free oxyhemoglobin in vivo...
  45. pmc Fetal hemoglobin silencing in humans
    Patricia A Oneal
    Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 10 Center Drive, Bldg 10, Rm 9B17, Bethesda, MD 20892, USA
    Blood 108:2081-6. 2006
    ..The silencing phenomenon is mediated by a mechanism of cellular replacement. This novel silencing pattern may be important for the development of HbF-enhancing therapies...
  46. ncbi request reprint Cloning of the human erythropoietin receptor gene
    C T Noguchi
    Laboratory of Chemical Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892
    Blood 78:2548-56. 1991
    ....
  47. ncbi request reprint The measurement of blood and plasma nitrite by chemiluminescence: pitfalls and solutions
    Mildred M Pelletier
    Molecular Medicine Branch, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 41:541-8. 2006
    ....
  48. pmc Red blood cell magnetophoresis
    Maciej Zborowski
    Department of Biomedical Engineering ND20, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44915, USA
    Biophys J 84:2638-45. 2003
    ..Magnetophoresis may open up an approach to characterize and separate cells for biochemical analysis based on intrinsic and extrinsic magnetic properties of biological macromolecules...
  49. pmc Hypoxia, red blood cells, and nitrite regulate NO-dependent hypoxic vasodilation
    Jack H Crawford
    Department of Pathology, Center for Free Radical Biology, University of Alabama at Birmingham, 35294, USA
    Blood 107:566-74. 2006
    ..These data support a function for RBC hemoglobin as an allosterically and redox-regulated nitrite reductase whose "enzyme activity" couples hypoxia to increased NO-dependent blood flow...
  50. ncbi request reprint NO contest: nitrite versus S-nitroso-hemoglobin
    Mark T Gladwin
    Circ Res 94:851-5. 2004
  51. pmc Identification and characterization of mechanistically distinct inducers of gamma-globin transcription
    John D Haley
    OSI Pharmaceuticals Inc, Farmingdale, NY 11735, USA
    Biochem Pharmacol 66:1755-68. 2003
    ..This screening methodology successfully identifies active compounds for further mechanistic and preclinical evaluation as potential therapeutic agents for sickle cell anemia...
  52. ncbi request reprint Plasma nitrite concentrations reflect the degree of endothelial dysfunction in humans
    Petra Kleinbongard
    Division of Cardiology, Pulmonary Diseases and Angiology, Department of Medicine, Heinrich Heine University, D 40225 Dusseldorf, Germany
    Free Radic Biol Med 40:295-302. 2006
    ..Future studies are necessary to identify the prognostic relevance of plasma nitrite determination in patients suffering from cardiovascular disease...
  53. ncbi request reprint Effects of iron nitrosylation on sickle cell hemoglobin solubility
    Xiuli Xu
    Department of Physics, Wake Forest University, Winston Salem, North Carolina 27109, USA
    J Biol Chem 277:36787-92. 2002
    ..Given the small amounts of NO-ligated hemoglobin achievable through any kind of NO therapy, we conclude that NO therapy does not benefit patients through any direct solubilizing effect...
  54. doi request reprint Effects of nitric oxide on red blood cell development and phenotype
    Vladan P Cokic
    Laboratory of Experimental Hematology, Institute for Medical Research, Belgrade, Serbia
    Curr Top Dev Biol 82:169-215. 2008
    ....
  55. ncbi request reprint How do red blood cells dilate blood vessels?--Reply
    Daniel B Kim-Shapiro
    Circ Res 95:e10. 2004
  56. pmc The reaction between nitrite and oxyhemoglobin: a mechanistic study
    Agnes Keszler
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Biol Chem 283:9615-22. 2008
    ..The consequences of uncoupling the roles of H(2)O(2) and NO(2) in the reaction mechanism for the in vivo reactivity of nitrite are discussed...
  57. pmc Hydroxyurea nitrosylates and activates soluble guanylyl cyclase in human erythroid cells
    Vladan P Cokic
    Laboratory of Experimental Hematology, Institute for Medical Research, Belgrade, Serbia
    Blood 111:1117-23. 2008
    ..These data add to an expanding appreciation of the role of hydroxyurea as an inducer of the NO/cGMP pathway in EPCs. These mechanisms may also be involved in the cytostatic effects of hydroxyurea, as well as the induction of HbF...
  58. pmc Nitric oxide from nitrite reduction by hemoglobin in the plasma and erythrocytes
    Kejing Chen
    Department of Biomedical Engineering, Johns Hopkins University School of Medicine, 613 Traylor Building, 720 Rutland Avenue, Baltimore, MD 21205, USA
    Nitric Oxide 18:47-60. 2008
    ..mM in erythrocyte); (3) intraerythrocytic hemoglobin encapsulated by a NO-resistant membrane is the major source of NO from nitrite reduction, and cell-free hemoglobin is a significant scavenger of both paracrine and endocrine NO...
  59. ncbi request reprint Unraveling the reactions of nitric oxide, nitrite, and hemoglobin in physiology and therapeutics
    Daniel B Kim-Shapiro
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Arterioscler Thromb Vasc Biol 26:697-705. 2006
    ..As subsequently understood, the hypoxic generation of NO from nitrite is likely to prove important in many aspects of physiology, pathophysiology, and therapeutics...
  60. ncbi request reprint Hydroxyurea increases eNOS protein levels through inhibition of proteasome activity
    Vladan P Cokic
    Laboratory of Experimental Hematology, Institute for Medical Research, Dr Subotica 4, P O Box 102, 11129 Belgrade, Serbia
    Nitric Oxide 16:371-8. 2007
    ..The NO production induced by hydroxyurea in endothelial cells appears to be mediated by long term posttranscriptional augmentation in eNOS levels via inhibition of the proteasome activity...
  61. ncbi request reprint Hydroxyurea induces the eNOS-cGMP pathway in endothelial cells
    Vladan P Cokic
    Institute of Medical Research, and the Institute of Endocrinology, Diabetes and Digestive and Diseases of Metabolism, Belgrade, Serbia and Montenegro
    Blood 108:184-91. 2006
    ..These studies established an additional mechanism by which rapid and sustained effects of hydroxyurea may affect cellular NO levels and perhaps enhance the effect of NO in myeloproliferative diseases...
  62. ncbi request reprint Electron paramagnetic resonance analysis of nitrosylhemoglobin in humans during NO inhalation
    Barbora Piknova
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 280:40583-8. 2005
    ..v) The half-time of HbNO in vivo is approximately 40 min...
  63. ncbi request reprint To publish or not to publish?
    Alan N Schechter
    J Lab Clin Med 145:9-11. 2005