R M Schaaper

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc A critical role for the putative NCS2 nucleobase permease YjcD in the sensitivity of Escherichia coli to cytotoxic and mutagenic purine analogs
    Stanislav G Kozmin
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA
    MBio 4:e00661-13. 2013
  2. pmc TusA (YhhP) and IscS are required for molybdenum cofactor-dependent base-analog detoxification
    Stanislav G Kozmin
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, 27709
    Microbiologyopen 2:743-55. 2013
  3. pmc Mutational consequences of dNTP pool imbalances in E. coli
    Roel M Schaaper
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, 111 TW Alexander Drive, Research Triangle Park, NC 27709, USA
    DNA Repair (Amst) 12:73-9. 2013
  4. ncbi request reprint The antimutator phenotype of E. coli mud is only apparent and results from delayed appearance of mutants
    R M Schaaper
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, P O Box 12233, 111 TW Alexander Drive, Research Triangle Park, NC 27709, USA
    Mutat Res 480:71-5. 2001
  5. ncbi request reprint Effect of Escherichia coli dnaE antimutator mutants on mutagenesis by the base analog N4-aminocytidine
    R M Schaaper
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, P O Box 12233, Research Triangle Park, NC 27709, USA
    Mutat Res 402:23-8. 1998
  6. pmc Antimutator mutants in bacteriophage T4 and Escherichia coli
    R M Schaaper
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Genetics 148:1579-85. 1998
  7. pmc Mutational analysis of the 3'-->5' proofreading exonuclease of Escherichia coli DNA polymerase III
    S A Taft-Benz
    Laboratory of Molecular Genetics, National Institute of Environmental Health Science, PO Box 12233, Research Triangle Park, NC 27709, USA
    Nucleic Acids Res 26:4005-11. 1998
  8. ncbi request reprint Suppressors of Escherichia coli mutT: antimutators for DNA replication errors
    R M Schaaper
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Mutat Res 350:17-23. 1996
  9. pmc Genetic requirements and mutational specificity of the Escherichia coli SOS mutator activity
    I J Fijalkowska
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    J Bacteriol 179:7435-45. 1997
  10. ncbi request reprint The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro
    P T Pham
    Laboratory of Molecular Genetics, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 273:23575-84. 1998

Collaborators

  • D Li
  • R E London
  • FRED PERRINO
  • Youri I Pavlov
  • Stanislav G Kozmin
  • I J Fijalkowska
  • Elena I Stepchenkova
  • P T Pham
  • Damian Gawel
  • S A Taft-Benz
  • P Jonczyk
  • C S McHenry
  • Stephen C Chow
  • M S Song
  • Iwona J Fijalkowska
  • Piotr Jonczyk
  • S G Kozmin
  • Jian Wang
  • M Maliszewska-Tkaczyk
  • R L Dunn
  • M Bialoskorska
  • M W Olson
  • R G Fowler
  • J Y Mo
  • M Olson
  • J R Carter
  • M A Franden
  • A R Oller
  • I J FijaƂkowska
  • Z Ciesla
  • A Nowicka
  • M M Tkaczyk

Detail Information

Publications27

  1. pmc A critical role for the putative NCS2 nucleobase permease YjcD in the sensitivity of Escherichia coli to cytotoxic and mutagenic purine analogs
    Stanislav G Kozmin
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA
    MBio 4:e00661-13. 2013
    ..We also present data showing that this protein may, in fact, also be a principal permease involved in transport of the normal purines guanine, hypoxanthine, and/or xanthine...
  2. pmc TusA (YhhP) and IscS are required for molybdenum cofactor-dependent base-analog detoxification
    Stanislav G Kozmin
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, 27709
    Microbiologyopen 2:743-55. 2013
    ..Overall, our data imply a novel and indispensable role of the IscS/TusA complex in the activity of several molybdoenzymes...
  3. pmc Mutational consequences of dNTP pool imbalances in E. coli
    Roel M Schaaper
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, 111 TW Alexander Drive, Research Triangle Park, NC 27709, USA
    DNA Repair (Amst) 12:73-9. 2013
    ....
  4. ncbi request reprint The antimutator phenotype of E. coli mud is only apparent and results from delayed appearance of mutants
    R M Schaaper
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, P O Box 12233, 111 TW Alexander Drive, Research Triangle Park, NC 27709, USA
    Mutat Res 480:71-5. 2001
    ..This delay likely results from the combined stress exerted by the adenine deficiency and the presence of the selective agent (i.e. valine)...
  5. ncbi request reprint Effect of Escherichia coli dnaE antimutator mutants on mutagenesis by the base analog N4-aminocytidine
    R M Schaaper
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, P O Box 12233, Research Triangle Park, NC 27709, USA
    Mutat Res 402:23-8. 1998
    ..The results show that mutagenesis by 4AC is unaffected by defects in either system. The combined results point to the critical role of the DNA polymerase in preventing mutations by base analogs...
  6. pmc Antimutator mutants in bacteriophage T4 and Escherichia coli
    R M Schaaper
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Genetics 148:1579-85. 1998
    ....
  7. pmc Mutational analysis of the 3'-->5' proofreading exonuclease of Escherichia coli DNA polymerase III
    S A Taft-Benz
    Laboratory of Molecular Genetics, National Institute of Environmental Health Science, PO Box 12233, Research Triangle Park, NC 27709, USA
    Nucleic Acids Res 26:4005-11. 1998
    ..Together, the data support the functional significance of the proposed motifs, presumably in catalysis, and suggest that the C-terminus of straightepsilon may be specifically involved in binding to the alpha (polymerase) subunit...
  8. ncbi request reprint Suppressors of Escherichia coli mutT: antimutators for DNA replication errors
    R M Schaaper
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Mutat Res 350:17-23. 1996
    ..8-oxoG) transversion mispairs and A.C or T.G transition mispairs. The finding may also have significance for teh interpretation of the antimutator effect conferred by these dnaE alleles in a wild-type (mut+) background...
  9. pmc Genetic requirements and mutational specificity of the Escherichia coli SOS mutator activity
    I J Fijalkowska
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    J Bacteriol 179:7435-45. 1997
    ..The modified complex forces extension of the mismatch largely at the exclusion of proofreading and polymerase dissociation pathways. SOS mutagenesis targeted at replication-blocking DNA lesions likely proceeds in the same manner...
  10. ncbi request reprint The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro
    P T Pham
    Laboratory of Molecular Genetics, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 273:23575-84. 1998
    ....
  11. ncbi request reprint The Escherichia coli galK2 papillation assay: its specificity and application to seven newly isolated mutator strains
    A R Oller
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709
    Mutat Res 292:175-85. 1993
    ..These classes may represent different mutational mechanisms that include defective base insertion, defective proofreading and interference with the postreplicative mismatch-repair system...
  12. pmc The C-terminal domain of dnaQ contains the polymerase binding site
    S A Taft-Benz
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    J Bacteriol 181:2963-5. 1999
    ..The data are consistent with the existence of two functional domains in epsilon, with the C-terminal domain responsible for polymerase binding...
  13. pmc Mutants in the Exo I motif of Escherichia coli dnaQ: defective proofreading and inviability due to error catastrophe
    I J Fijalkowska
    Laboratory of Molecualr Genetics, NationalInstitute of Enviromental Health Sciences, Research Triangle Park, NC 27709, USA
    Proc Natl Acad Sci U S A 93:2856-61. 1996
    ..The probability of lethality by excessive mutation is supported by calculations estimating the number of inactivating mutations in essential genes per chromosome replication...
  14. pmc Effects of Escherichia coli dnaE antimutator alleles in a proofreading-deficient mutD5 strain
    I J Fijalkowska
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    J Bacteriol 177:5979-86. 1995
    ..T-->T.A transversion in this background. A model suggesting how the dnaE antimutator alleles might exert their effects in proofreading-proficient and -deficient backgrounds is presented...
  15. ncbi request reprint Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III
    J Y Mo
    Laboratory of Molecular Genetics, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 271:18947-53. 1996
    ..A comparison to in vivo mutational spectra suggests that the pol III accessory factors may play a major role in modulating the fidelity of DNA synthesis...
  16. pmc Antimutator mutations in the alpha subunit of Escherichia coli DNA polymerase III: identification of the responsible mutations and alignment with other DNA polymerases
    I J Fijalkowska
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709
    Genetics 134:1039-44. 1993
    ..This and other observations suggest that the effect of these mutations may be exerted indirectly through effects on polymerase conformation and/or DNA/polymerase interactions...
  17. ncbi request reprint Mismatch extension by Escherichia coli DNA polymerase III holoenzyme
    P T Pham
    Laboratory of Molecular Genetics, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 274:3705-10. 1999
    ..The preference of polymerase III HE for misalignment extension over direct mismatch extension provides a basis for explaining the in vitro (-1)-frameshift specificity of polymerase III HE...
  18. pmc Transcription-repair coupling determines the strandedness of ultraviolet mutagenesis in Escherichia coli
    A R Oller
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709
    Proc Natl Acad Sci U S A 89:11036-40. 1992
    ....
  19. ncbi request reprint A preliminary CD and NMR study of the Escherichia coli DNA polymerase III theta subunit
    D Li
    Laboratory of Structural Biology, NIEHS, Research Triangle Park, North Carolina 27709, USA
    Proteins 36:111-6. 1999
    ..Further work is currently in progress to define the solution structure of theta and the theta-epsilon186 complex...
  20. pmc dnaX36 Mutator of Escherichia coli: effects of the {tau} subunit of the DNA polymerase III holoenzyme on chromosomal DNA replication fidelity
    Damian Gawel
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    J Bacteriol 193:296-300. 2011
    ..We have shown that this fidelity function (i) applies to both leading- and lagging-strand synthesis, (ii) is independent of Pol IV, and (iii) is limited by Pol II...
  21. pmc Role for CysJ flavin reductase in molybdenum cofactor-dependent resistance of Escherichia coli to 6-N-hydroxylaminopurine
    Stanislav G Kozmin
    Laboratory of Molecular Genetics, MD E3 01, National Institute of Environmental Health Sciences, 111 TW Alexander Drive, Research Triangle Park, NC 27709, USA
    J Bacteriol 192:2026-33. 2010
    ..In support of the proposed interaction of the CysJ and YcbX proteins, we show that an apparent CysJ-YcbX "hybrid" protein from two Vibrio species is capable of compensating for a double cysJ ycbX defect in E. coli...
  22. pmc SOS mutator activity: unequal mutagenesis on leading and lagging strands
    M Maliszewska-Tkaczyk
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland
    Proc Natl Acad Sci U S A 97:12678-83. 2000
    ..We suggest that, for the lacZ target used in this study, SOS mutator activity operates with very different efficiency in the two strands. Specifically, the lagging strand of replication appears most susceptible to the SOS mutator effect...
  23. pmc In vivo protein interactions within the Escherichia coli DNA polymerase III core
    P Jonczyk
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
    J Bacteriol 180:1563-6. 1998
    ..Thus, the mutator phenotype of dnaQ49 may be the result of an altered conformation of the epsilon protein, which leads to altered interactions within the Pol III core...
  24. pmc Unequal fidelity of leading strand and lagging strand DNA replication on the Escherichia coli chromosome
    I J Fijalkowska
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02 106 Warsaw, Pawinskiego 5A, Poland
    Proc Natl Acad Sci U S A 95:10020-5. 1998
    ..Analysis of the possible mispairs underlying each specific base pair substitution suggests that the lagging strand replication on the E. coli chromosome may be more accurate than leading strand replication...
  25. ncbi request reprint The delta and delta ' subunits of the DNA polymerase III holoenzyme are essential for initiation complex formation and processive elongation
    M S Song
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    J Biol Chem 276:35165-75. 2001
    ....
  26. pmc Hypersensitivity of Escherichia coli Delta(uvrB-bio) mutants to 6-hydroxylaminopurine and other base analogs is due to a defect in molybdenum cofactor biosynthesis
    S G Kozmin
    Department of Genetics, Sankt Petersburg State University, Saint Petersburg, Russia
    J Bacteriol 182:3361-7. 2000
    ..We propose that the molybdenum cofactor is required as a cofactor for an as yet unidentified enzyme (or enzymes) that acts to inactivate HAP and other related compounds...
  27. ncbi request reprint The role of the mutT gene of Escherichia coli in maintaining replication fidelity
    R G Fowler
    Department of Biological Sciences, San Jose State University, CA 95192, USA
    FEMS Microbiol Rev 21:43-54. 1997
    ..MutT is assumed to hydrolyze 8-oxodGTP in the nucleotide pool before it can be misincorporated. While the broad role of MutT in error avoidance seems resolved, important details that are still unclear are pointed out in this review...