D L Sackett
Affiliation: National Institutes of Health
Suen L, Tekle Smith M, Williamson K, Infantine J, Reznik S, Tanis P, et al
. Design and 22-step synthesis of highly potent D-ring modified and linker-equipped analogs of spongistatin 1. Nat Commun. 2018;9:4710 pubmed publisher
Ismalaj T, Sackett D. An inexpensive replacement for dry ice in the laboratory. Anal Biochem. 2015;474:38-9 pubmed publisher
..Following use, the material is returned to the freezer to re-chill until the next usage. ..
Montecinos Franjola F, Schuck P, Sackett D. Tubulin Dimer Reversible Dissociation: AFFINITY, KINETICS, AND DEMONSTRATION OF A STABLE MONOMER. J Biol Chem. 2016;291:9281-94 pubmed publisher
..Monomer-monomer association is much less sensitive than dimer-dimer association to solution changes (GTP/GDP, urea, and trimethylamine oxide). ..
Woods L, Arico J, Frein J, Sackett D, Taylor R. Synthesis and Biological Evaluation of 7-Deoxy-Epothilone Analogues. Int J Mol Sci. 2017;18: pubmed publisher
Sheldon K, Gurnev P, Bezrukov S, Sackett D. Tubulin tail sequences and post-translational modifications regulate closure of mitochondrial voltage-dependent anion channel (VDAC). J Biol Chem. 2015;290:26784-9 pubmed publisher
..Our results demonstrate that small changes in sequence or post-translational modification of the unstructured tails of tubulin result in substantial changes in VDAC closure. ..
Sackett D, Ruvinov S, Thompson J. N5-(L-1-carboxyethyl)-L-ornithine synthase: physical and spectral characterization of the enzyme and its unusual low pKa fluorescent tyrosine residues. Protein Sci. 1999;8:2121-9 pubmed
..Possible roles in catalysis for the chymotrypsin sensitive loop and for the low pKa Tyr residues are discussed. ..