T Rouault

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Distinct iron-sulfur cluster assembly complexes exist in the cytosol and mitochondria of human cells
    W H Tong
    National Institute of Child Health and Human Development, Cell Biology and Metabolism Branch, Bethesda, MD 20892, USA
    EMBO J 19:5692-700. 2000
  2. ncbi request reprint Functions of mitochondrial ISCU and cytosolic ISCU in mammalian iron-sulfur cluster biogenesis and iron homeostasis
    Wing Hang Tong
    National Institute of Child Health and Human Development, Cell Biology and Metabolism Branch, Bethesda, Maryland 20892, USA
    Cell Metab 3:199-210. 2006
  3. ncbi request reprint Brain iron metabolism
    Tracey A Rouault
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Semin Pediatr Neurol 13:142-8. 2006
  4. ncbi request reprint The role of iron regulatory proteins in mammalian iron homeostasis and disease
    Tracey A Rouault
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Building 18T, Room 101, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Chem Biol 2:406-14. 2006
  5. ncbi request reprint Iron-sulphur cluster biogenesis and mitochondrial iron homeostasis
    Tracey A Rouault
    National Institute of Child Health and Human Development, Cell Biology and Metabolism Branch, Bethesda, Maryland 20892, USA
    Nat Rev Mol Cell Biol 6:345-51. 2005
  6. ncbi request reprint Microbiology. Pathogenic bacteria prefer heme
    Tracey A Rouault
    Section on Human Iron Metabolism, Cell Biology and Metabolism Branch, The National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 305:1577-8. 2004
  7. ncbi request reprint Biochemistry. If the RNA fits, use it
    Tracey A Rouault
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Science 314:1886-7. 2006
  8. pmc How mammals acquire and distribute iron needed for oxygen-based metabolism
    Tracey A Rouault
    Cell Biology and Metabolism Branch of the National Institute of Child Health and Human Development in Bethesda, Maryland, USA
    PLoS Biol 1:E79. 2003
  9. ncbi request reprint Hepatic iron overload in alcoholic liver disease: why does it occur and what is its role in pathogenesis?
    Tracey A Rouault
    Section on Human Iron Metabolism, Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Building 18, Room 101, National Institutes of Health, Bethesda, MD 20892, USA
    Alcohol 30:103-6. 2003
  10. ncbi request reprint Post-transcriptional regulation of human iron metabolism by iron regulatory proteins
    Tracey A Rouault
    Section on Human Iron Metabolism, Cell Biology and Metabolism Branch, NICHD NIH, Building 18, Room 101, Bethesda, MD 20892, USA
    Blood Cells Mol Dis 29:309-14. 2002

Collaborators

Detail Information

Publications50

  1. pmc Distinct iron-sulfur cluster assembly complexes exist in the cytosol and mitochondria of human cells
    W H Tong
    National Institute of Child Health and Human Development, Cell Biology and Metabolism Branch, Bethesda, MD 20892, USA
    EMBO J 19:5692-700. 2000
    ..The possible roles of the IscU proteins in mammalian cells and the potential implications of compartmentalization of Fe-S cluster assembly are discussed...
  2. ncbi request reprint Functions of mitochondrial ISCU and cytosolic ISCU in mammalian iron-sulfur cluster biogenesis and iron homeostasis
    Wing Hang Tong
    National Institute of Child Health and Human Development, Cell Biology and Metabolism Branch, Bethesda, Maryland 20892, USA
    Cell Metab 3:199-210. 2006
    ..These results provide evidence for a coordinated response to iron deficiency that includes activation of iron uptake, redistribution of intracellular iron, and decreased utilization of iron in Fe-S proteins...
  3. ncbi request reprint Brain iron metabolism
    Tracey A Rouault
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Semin Pediatr Neurol 13:142-8. 2006
    ....
  4. ncbi request reprint The role of iron regulatory proteins in mammalian iron homeostasis and disease
    Tracey A Rouault
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Building 18T, Room 101, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Chem Biol 2:406-14. 2006
    ..The early death of mouse embryos that lack both IRP1 and IRP2 suggests a central role for IRP-mediated regulation in cellular viability...
  5. ncbi request reprint Iron-sulphur cluster biogenesis and mitochondrial iron homeostasis
    Tracey A Rouault
    National Institute of Child Health and Human Development, Cell Biology and Metabolism Branch, Bethesda, Maryland 20892, USA
    Nat Rev Mol Cell Biol 6:345-51. 2005
    ..Recent studies in model systems and humans reveal that iron-sulphur proteins have important roles in mitochondrial iron homeostasis and in the pathogenesis of the human disease Friedreich ataxia...
  6. ncbi request reprint Microbiology. Pathogenic bacteria prefer heme
    Tracey A Rouault
    Section on Human Iron Metabolism, Cell Biology and Metabolism Branch, The National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 305:1577-8. 2004
  7. ncbi request reprint Biochemistry. If the RNA fits, use it
    Tracey A Rouault
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Science 314:1886-7. 2006
  8. pmc How mammals acquire and distribute iron needed for oxygen-based metabolism
    Tracey A Rouault
    Cell Biology and Metabolism Branch of the National Institute of Child Health and Human Development in Bethesda, Maryland, USA
    PLoS Biol 1:E79. 2003
  9. ncbi request reprint Hepatic iron overload in alcoholic liver disease: why does it occur and what is its role in pathogenesis?
    Tracey A Rouault
    Section on Human Iron Metabolism, Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Building 18, Room 101, National Institutes of Health, Bethesda, MD 20892, USA
    Alcohol 30:103-6. 2003
    ..However, it is not known why hepatic iron accumulation occurs or how it contributes to disease progression. In this review, information about the role of iron in the pathophysiology of liver disease is reviewed and discussed...
  10. ncbi request reprint Post-transcriptional regulation of human iron metabolism by iron regulatory proteins
    Tracey A Rouault
    Section on Human Iron Metabolism, Cell Biology and Metabolism Branch, NICHD NIH, Building 18, Room 101, Bethesda, MD 20892, USA
    Blood Cells Mol Dis 29:309-14. 2002
    ..We have studied the role of these proteins in knockout mice and discovered that misregulation of iron metabolism can be a primary cause of neurodegeneration...
  11. doi request reprint Tangled up in red: intertwining of the heme and iron-sulfur cluster biogenesis pathways
    Tracey A Rouault
    Molecular Medicine Program, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Cell Metab 10:80-1. 2009
    ..Several are implicated in iron-sulfur cluster biogenesis, and malfunction of these genes may repress heme synthesis by activating the IRE/IRP posttranscriptional regulatory system...
  12. pmc Human iron-sulfur cluster assembly, cellular iron homeostasis, and disease
    Hong Ye
    Molecular Medicine Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 49:4945-56. 2010
    ....
  13. pmc Human ISD11 is essential for both iron-sulfur cluster assembly and maintenance of normal cellular iron homeostasis
    Yanbo Shi
    National Institute of Child Health and Human Development, Molecular Medicine Program, Bethesda, MD 20892, USA
    Hum Mol Genet 18:3014-25. 2009
    ..These results indicate that ISD11 is important in the biogenesis of Fe-S clusters in mammalian cells, and its loss disrupts normal mitochondrial and cytosolic iron homeostasis...
  14. ncbi request reprint Compartment-specific protection of iron-sulfur proteins by superoxide dismutase
    Fanis Missirlis
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 278:47365-9. 2003
    ....
  15. ncbi request reprint Mammalian tissue oxygen levels modulate iron-regulatory protein activities in vivo
    Esther G Meyron-Holtz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Science 306:2087-90. 2004
    ..Thus, IRP2 dominates regulation of mammalian iron homeostasis because it alone registers iron concentrations and modulates its RNA-binding activity at physiological oxygen tensions...
  16. pmc Iron-dependent regulation of frataxin expression: implications for treatment of Friedreich ataxia
    Kuanyu Li
    National Institute of Child Health and Human Development, Molecular Medicine Program, Bethesda, MD 20892, USA
    Hum Mol Genet 17:2265-73. 2008
    ..Thus, our results imply that therapeutic efforts should focus on an approach that combines iron removal from mitochondria with a treatment that increases cytosolic iron levels to maximize residual frataxin expression in FA patients...
  17. ncbi request reprint Roles of the mammalian cytosolic cysteine desulfurase, ISCS, and scaffold protein, ISCU, in iron-sulfur cluster assembly
    Kuanyu Li
    NICHD, National Institutes of Health, Intramural Program, Bethesda, Maryland 20892, USA
    J Biol Chem 281:12344-51. 2006
    ..Thus, the cytosolic form of ISCS is a functional cysteine desulfurase that can collaborate with cytosolic ISCU to promote de novo iron-sulfur cluster formation...
  18. pmc Characterization of mitochondrial ferritin in Drosophila
    Fanis Missirlis
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:5893-8. 2006
    ..The conservation of mitochondrial ferritin in an insect species underscores the importance of this iron-storage molecule...
  19. ncbi request reprint Renal iron metabolism: transferrin iron delivery and the role of iron regulatory proteins
    Deliang Zhang
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    J Am Soc Nephrol 18:401-6. 2007
  20. ncbi request reprint Metabolic regulation of citrate and iron by aconitases: role of iron-sulfur cluster biogenesis
    Wing Hang Tong
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, NIH Bldg 18, Rm 101, Bethesda, MD 20892, USA
    Biometals 20:549-64. 2007
    ..This review discusses the central role of aconitases in intermediary metabolism and explores how iron homeostasis and Fe-S cluster biogenesis regulate the Fe-S cluster switch and modulate intracellular citrate flux...
  21. pmc Glutaredoxin 5 deficiency causes sideroblastic anemia by specifically impairing heme biosynthesis and depleting cytosolic iron in human erythroblasts
    Hong Ye
    Molecular Medicine Program, National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA
    J Clin Invest 120:1749-61. 2010
    ..Erythroblasts express both IRP-repressible ALAS2 and non-IRP-repressible ferroportin 1b. The unique combination of IRP targets likely accounts for the tissue-specific phenotype of human GLRX5 deficiency...
  22. pmc Homeostatic mechanisms for iron storage revealed by genetic manipulations and live imaging of Drosophila ferritin
    Fanis Missirlis
    Neural Cell Fate Determinants Section, National Institute of Neurological Disorders and Stroke, Bethesda, Maryland 20892, USA
    Genetics 177:89-100. 2007
    ..Our study revealed both conserved features and insect-specific adaptations of ferritin nanocages and provides novel imaging possibilities for their in vivo characterization...
  23. ncbi request reprint Self-association and ligand-induced conformational changes of iron regulatory proteins 1 and 2
    Emine Yikilmaz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 5766, USA
    Biochemistry 44:8470-8. 2005
    ..The dynamic change of the IRP between different oligomeric and conformational states induced by interaction with IRE may play a role in the iron regulatory functions of IRPs...
  24. pmc Splice mutation in the iron-sulfur cluster scaffold protein ISCU causes myopathy with exercise intolerance
    Fanny Mochel
    Developmental and Metabolic Neurology Branch, NINDS, NIH, Bethesda, MD 20892, USA
    Am J Hum Genet 82:652-60. 2008
    ..ISCU interacts with the Friedreich ataxia gene product frataxin in iron-sulfur cluster biosynthesis. Our results therefore extend the range of known human diseases that are caused by defects in iron-sulfur cluster biogenesis...
  25. pmc Iron-sulfur cluster biogenesis and human disease
    Tracey A Rouault
    Molecular Medicine Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Trends Genet 24:398-407. 2008
    ..Thus, defects in the iron-sulfur cluster biogenesis pathway could underlie many human diseases...
  26. pmc Genetic ablations of iron regulatory proteins 1 and 2 reveal why iron regulatory protein 2 dominates iron homeostasis
    Esther G Meyron-Holtz
    Cell Biology and Metabolism Branch, NIHCD, Bethesda, MD, USA
    EMBO J 23:386-95. 2004
    ..Thus, IRP2 dominates post-transcriptional regulation of iron metabolism in mammals...
  27. ncbi request reprint Expression of the iron transporter ferroportin in synaptic vesicles and the blood-brain barrier
    Laura Jui chen Wu
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Brain Res 1001:108-17. 2004
    ....
  28. pmc Brain iron homeostasis, the choroid plexus, and localization of iron transport proteins
    Tracey A Rouault
    Molecular Medicine Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Metab Brain Dis 24:673-84. 2009
    ....
  29. pmc A ferroportin transcript that lacks an iron-responsive element enables duodenal and erythroid precursor cells to evade translational repression
    De Liang Zhang
    Molecular Medicine Program, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Cell Metab 9:461-73. 2009
    ..In erythroid precursor cells, we hypothesize that FPN1B expression enhances real-time sensing of systemic iron status and facilitates restriction of erythropoiesis in response to low systemic iron...
  30. ncbi request reprint Severity of neurodegeneration correlates with compromise of iron metabolism in mice with iron regulatory protein deficiencies
    Sophia R Smith
    National Institute of Child Health and Human Development, Cell Biology and Metabolism Branch, Bethesda, Maryland 20892, USA
    Ann N Y Acad Sci 1012:65-83. 2004
    ..These observations suggest that therapeutic strategies that aim to revitalize neurons by treatment with neurotrophic factors may be of value in IRP2-/- and IRP1+/- IRP2-/- mouse models of neurodegeneration...
  31. ncbi request reprint The intestinal heme transporter revealed
    Tracey A Rouault
    Cell Biology and Metabolism Branch, NICHD, Bethesda, MD 20892, USA
    Cell 122:649-51. 2005
    ..Their identification of heme carrier protein 1 (HCP1) provides a major missing piece in our understanding of iron uptake and mammalian nutrition...
  32. pmc Microcytic anemia, erythropoietic protoporphyria, and neurodegeneration in mice with targeted deletion of iron-regulatory protein 2
    Sharon S Cooperman
    National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Blood 106:1084-91. 2005
    ....
  33. ncbi request reprint Complete loss of iron regulatory proteins 1 and 2 prevents viability of murine zygotes beyond the blastocyst stage of embryonic development
    Sophia R Smith
    Section on Human Iron Metabolism, Cell Biology and Metabolism Branch National Institute of Child Health and Human Development Bethesda, MD 20892, USA
    Blood Cells Mol Dis 36:283-7. 2006
    ..These results demonstrate that IRPs are indispensable for regulation of mammalian iron homeostasis at the post-implantation stage of murine embryonic development...
  34. ncbi request reprint Iron regulatory protein 2 as iron sensor. Iron-dependent oxidative modification of cysteine
    Dae Kyung Kang
    Laboratory of Biochemistry, NHLBI, National Institutes of Health and the Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 278:14857-64. 2003
    ..One cysteine residue is oxidized to dehydrocysteine and other products. This covalent modification may thus mark the protein molecule for degradation by the proteasome system...
  35. ncbi request reprint The role of endogenous heme synthesis and degradation domain cysteines in cellular iron-dependent degradation of IRP2
    Emmanuel Bourdon
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Blood Cells Mol Dis 31:247-55. 2003
    ..Thus, we could not discern a role for the recently defined in vitro cysteine-dependent iron-binding site of IRP2 in cellular physiology. The early molecular events in iron-dependent degradation of IRP2 remain to be elucidated...
  36. pmc Tempol-mediated activation of latent iron regulatory protein activity prevents symptoms of neurodegenerative disease in IRP2 knockout mice
    Manik C Ghosh
    National Institute of Child Health and Human Development and Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 105:12028-33. 2008
    ....
  37. ncbi request reprint MRI detection of ferritin iron overload and associated neuronal pathology in iron regulatory protein-2 knockout mice
    Colette Grabill
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bldg 18T, Room 101 9000 Rockville Pike, Bethesda, MD 20892, USA
    Brain Res 971:95-106. 2003
    ..Our discoveries may have significant applications for the diagnosis and treatment of human diseases if such high-resolution techniques can be adapted for use in human subjects...
  38. pmc Electron tomography of degenerating neurons in mice with abnormal regulation of iron metabolism
    Peijun Zhang
    Laboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, MD 20817, USA
    J Struct Biol 150:144-53. 2005
    ....
  39. pmc Subcellular compartmentalization of human Nfu, an iron-sulfur cluster scaffold protein, and its ability to assemble a [4Fe-4S] cluster
    Wing Hang Tong
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 100:9762-7. 2003
    ....
  40. ncbi request reprint Numerous proteins in Mammalian cells are prone to iron-dependent oxidation and proteasomal degradation
    Steven K Drake
    National Institute of Child Health and Human Development, Bethesda, MD, USA
    Dev Neurosci 24:114-24. 2002
    ..As iron overload is a feature of many human neurological diseases, further characterization of the process of degradation of iron-dependently oxidized proteins may yield insights into mechanisms of human disease...
  41. pmc A high-capacity RNA affinity column for the purification of human IRP1 and IRP2 overexpressed in Pichia pastoris
    Charles R Allerson
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    RNA 9:364-74. 2003
    ..We demonstrate here the use of an IRE affinity column in the large-scale purification of IRP1 and IRP2, and suggest that the convenience of this approach will prove attractive in the analysis of other RNA-binding proteins...
  42. ncbi request reprint Identification of a heme-sensing domain in iron regulatory protein 2
    Jinsook Jeong
    Laboratory of Biochemistry, NHLBI, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 279:45450-4. 2004
    ..This covalent modification may thus mark the protein molecule for degradation by the proteasome system, providing another mechanism by which heme can regulate the level of iron regulatory protein 2...
  43. ncbi request reprint Of two cytosolic aconitases expressed in Drosophila, only one functions as an iron-regulatory protein
    Maria I Lind
    Department of Comparative Physiology, Evolutionary Biology Centre, Uppsala University, S 75236 Uppsala, Sweden
    J Biol Chem 281:18707-14. 2006
    ..Our data suggest that as a first step during the evolution of the IRP/IRE system, the ancient cytosolic aconitase was duplicated in insects with one variant acquiring IRE-specific binding...
  44. ncbi request reprint Identification of the ubiquitin-protein ligase that recognizes oxidized IRP2
    Koji Yamanaka
    Department of Molecular and System Biology, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Nat Cell Biol 5:336-40. 2003
    ..The oxidation of IRP2 is generated by haem, which binds to IRP2 in iron-rich cells, and by oxygen, indicating that the iron sensing of IRP2 depends on the synthesis and availability of haem...
  45. ncbi request reprint Manganese targets m-aconitase and activates iron regulatory protein 2 in AF5 GABAergic cells
    Daniel R Crooks
    Department of Environmental Toxicology, University of California, Santa Cruz, California 95064, USA
    J Neurosci Res 85:1797-809. 2007
    ..Together, these results demonstrate that m-aconitase is an important target of Mn and thatMn-induced alteration of iron homeostasis is mediated predominantly through IRP2...
  46. ncbi request reprint Linking physiological functions of iron
    Tracey A Rouault
    Nat Chem Biol 1:193-4. 2005
  47. ncbi request reprint An iron regulatory-like protein expressed in Plasmodium falciparum displays aconitase activity
    Marcus Hodges
    The Center for Sickle Cell Disease, Howard University, Washington, DC 20059, USA
    Mol Biochem Parasitol 143:29-38. 2005
    ..Further understanding of the role of PfIRPa/aconitase in the regulation of P. falciparum homeostasis may contribute towards the development of novel antimalarial strategies against plasmodial species...
  48. ncbi request reprint Expression of a recombinant IRP-like Plasmodium falciparum protein that specifically binds putative plasmodial IREs
    Mark Loyevsky
    Center for Sickle Cell Disease, Howard University, 2121 Georgia Avenue, NW, Washington, DC 20059, USA
    Mol Biochem Parasitol 126:231-8. 2003
    ..Endogenous PfIRPa retained all three putative 32P-IREs at the same position on the gel as the recombinant PfIRPa...
  49. ncbi request reprint Advancements in the pathophysiology of Friedreich's Ataxia and new prospects for treatments
    Ngolela E Babady
    Department of Pediatric and Adolescent Medicine, Mayo Clinic College of Medicine, Rochester, MN 55905, USA, and Bruce Lefroy Centre for Genetic Health Research, Murdoch Children s Research Institute, Royal Children s Hospital, Melbourne, Australia
    Mol Genet Metab 92:23-35. 2007
    ..Story Landis noted in her opening remarks for the conference that there was a "palpable sense of energy, excitement, and enthusiasm" over the scientific progress made since the FRDA gene was discovered over 10 years ago...
  50. ncbi request reprint Iron overload in Africans and African-Americans and a common mutation in the SCL40A1 (ferroportin 1) gene
    Victor R Gordeuk
    Howard University College of Medicine, Washington, DC 20059, USA
    Blood Cells Mol Dis 31:299-304. 2003
    ..We conclude that the Q248H mutation is a common polymorphism in the ferroportin 1 gene in African populations that may be associated with mild anemia and a tendency to iron loading...