Research Topics
Genomes and Genes
| Steven A RosenbergSummaryAffiliation: National Institutes of Health Country: USA Publications
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Detail Information
Publications
New directions in cellular therapy of cancer: a summary of the summit on cellular therapy for cancerDavid F Stroncek
Department of Transfusion Medicine, Clinical Center, NIH, Bethesda, USA
J Transl Med 10:48. 2012..In the future, combinations of adoptive transfer of T cells and specific vaccination against the cognate antigen can be envisaged to further enhance the effectiveness of these therapies...
Cancer immunotherapy: moving beyond current vaccinesSteven A Rosenberg
Surgery Branch of the Center for Cancer Research at the National Cancer Institute, Building 10, Room 2B42, 10 Center Drive, MSC 1502 Bethesda, Maryland 20892 1502, USA
Nat Med 10:909-15. 2004..6%), and comparable to the results obtained by others. We consider here results in cancer vaccine trials and highlight alternate strategies that mediate cancer regression in preclinical and clinical models...
Recombinant fowlpox viruses encoding the anchor-modified gp100 melanoma antigen can generate antitumor immune responses in patients with metastatic melanomaSteven A Rosenberg
Center for Cancer Research, Surgery Branch, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
Clin Cancer Res 9:2973-80. 2003..The immunogenicity of these constructs was studied using peripheral blood mononuclear cells to measure epitope-specific release of IFN-gamma...
Cancer regression and autoimmunity induced by cytotoxic T lymphocyte-associated antigen 4 blockade in patients with metastatic melanomaGiao Q Phan
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 100:8372-7. 2003..This study establishes CTLA-4 as an important molecule regulating tolerance to "self" antigens in humans and suggests a role for CTLA-4 blockade in breaking tolerance to human cancer antigens for cancer immunotherapy...
Immunization of patients with metastatic melanoma using both class I- and class II-restricted peptides from melanoma-associated antigensGiao Q Phan
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institute of Health, Bethesda, MD 20892-1502, USA
J Immunother (1997) 26:349-56. 2003..Moreover, the sequential, nonrandomized nature of patient enrollment for the two trials may account for the differences in immunologic response...
Gene therapy with human and mouse T-cell receptors mediates cancer regression and targets normal tissues expressing cognate antigenLaura A Johnson
Surgery Branch, Hatfield Clinical Research Center, National Cancer Institute NIH, Bethesda, MD 20892, USA
Blood 114:535-46. 2009..This trial was registered at www.ClinicalTrials.gov as NCI-07-C-0174 and NCI-07-C-0175...
Microbial translocation augments the function of adoptively transferred self/tumor-specific CD8+ T cells via TLR4 signalingChrystal M Paulos
National Cancer Institute NCI, NIH, Bethesda, Maryland 20892 1502, USA
J Clin Invest 117:2197-204. 2007..Thus, disruption of the homeostatic balance between the host and microbes can enhance cell-based tumor immunotherapy...
Presentation of tumor antigens by dendritic cells genetically modified with viral and nonviral vectorsMichal Lotem
Surgery Branch, National Cancer Institute, Bethesda, MD 20892, USA
J Immunother (1997) 29:616-27. 2006..These data suggest that DCs transduced with viral vectors may be more efficient than DCs transfected with cDNAs or RNAs for the induction of tumor reactive CD8+ and CD4+ T cells in vitro and in human vaccination trials...
Adoptive cell therapy for patients with metastatic melanoma: evaluation of intensive myeloablative chemoradiation preparative regimensMark E Dudley
Surgery Branch, National Cancer Institute, NIH, Bethesda, MD 20892 1201, USA
J Clin Oncol 26:5233-9. 2008..Here, we update that study and evaluate the safety and efficacy of two increased-intensity myeloablative lymphodepleting regimens...
CD8+ T cell immunity against a tumor/self-antigen is augmented by CD4+ T helper cells and hindered by naturally occurring T regulatory cellsPaul A Antony
Division of Surgery, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 174:2591-601. 2005..These findings reveal that Th cells can help break tolerance to a persisting self-Ag and treat established tumors through an IL-2-dependent mechanism, but requires simultaneous absence of naturally occurring T(reg) cells to be effective...
A high molecular weight melanoma-associated antigen-specific chimeric antigen receptor redirects lymphocytes to target human melanomasWilliam R Burns
Surgery Branch, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
Cancer Res 70:3027-33. 2010..This may represent a novel means to treat the majority of patients with advanced melanoma, most notably those unable to receive current ACT therapies...
Both CD4 and CD8 T cells mediate equally effective in vivo tumor treatment when engineered with a highly avid TCR targeting tyrosinaseTimothy L Frankel
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 184:5988-98. 2010..These results suggest that this highly active tyrosinase-specific TCR could be of value in adoptive cell transfer for melanoma...
Transfer of a TCR gene derived from a patient with a marked antitumor response conveys highly active T-cell effector functionsMarybeth S Hughes
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Hum Gene Ther 16:457-72. 2005..TCR-transduced T-cells are thus attractive candidates for evaluation in cell transfer therapies of patients with cancer...
Cancer regression in patients after transfer of genetically engineered lymphocytesRichard A Morgan
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
Science 314:126-9. 2006..This study suggests the therapeutic potential of genetically engineered cells for the biologic therapy of cancer...
IL-2 and IL-21 confer opposing differentiation programs to CD8+ T cells for adoptive immunotherapyChristian S Hinrichs
National Cancer Institute, Surgery Branch, National Institutes of Health, Bethesda, MD 20892 1502, USA
Blood 111:5326-33. 2008..Thus, the efficacy of CD8(+) T cells for adoptive immunotherapy can be influenced by opposing differentiation programs conferred by IL-2 and IL-21, a finding with important implications for the development of cellular cancer therapies...
High efficiency TCR gene transfer into primary human lymphocytes affords avid recognition of melanoma tumor antigen glycoprotein 100 and does not alter the recognition of autologous melanoma antigensRichard A Morgan
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 171:3287-95. 2003..These results suggest that lymphocytes genetically engineered to express anti-gp100 TCR may be of value in the adoptive immunotherapy of patients with melanoma...
Analysis of the cellular mechanism of antitumor responses and autoimmunity in patients treated with CTLA-4 blockadeAjay V Maker
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20814, USA
J Immunol 175:7746-54. 2005..Therefore, our results suggest that the antitumor effects of CTLA-4 blockade are due to increased T cell activation rather than inhibition or depletion of T regulatory cells...
Antiangiogenic agents can increase lymphocyte infiltration into tumor and enhance the effectiveness of adoptive immunotherapy of cancerRajeev K Shrimali
Surgery Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 1201, USA
Cancer Res 70:6171-80. 2010..These studies provide a rationale for the exploration of combining antiangiogenic agents with ACT for the treatment of patients with cancer...
T-cell receptor gene therapy of established tumors in a murine melanoma modelJohn D Abad
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunother 31:1-6. 2008..This model may be a powerful tool for evaluating future TCR gene transfer-based strategies...
CTLA-4 dysregulation of self/tumor-reactive CD8+ T-cell function is CD4+ T-cell dependentLuca Gattinoni
Center for Cancer Research, National Cancer Institute, National Institutes of Health, Mark O Hatfield Clinical Research Center, 10 Center Drive, Room 3 5750, Bethesda, MD 20892, USA
Blood 108:3818-23. 2006..These results indicated that CD8(+) CLTA-4(-/-) T-cell-mediated autoimmunity and tumor immunity required CD4(+) T cells in which the function was dysregulated by the absence of CTLA-4-mediated negative costimulation...
Cell transfer therapy for cancer: lessons from sequential treatments of a patient with metastatic melanomaSteven A Rosenberg
Center for Cancer Research, Surgery Branch, National Cancer Institute, National Institute of Health, Building 10, Room 2B42, 10 Center Drive, Bethesda, Maryland 20892, USA
J Immunother 26:385-93. 2003....
Human effector CD8+ T cells derived from naive rather than memory subsets possess superior traits for adoptive immunotherapyChristian S Hinrichs
National Cancer Institute, Bethesda, MD, USA
Blood 117:808-14. 2011..Thus, these data suggest that naive cells resist terminal differentiation, or "exhaustion," maintain high replicative potential, and therefore may be the superior subset for use in adoptive immunotherapy...
Multiple HLA class II-restricted melanocyte differentiation antigens are recognized by tumor-infiltrating lymphocytes from a patient with melanomaPaul F Robbins
Surgery Branch, National Cancer Institute, National Institutes of Health, Building 10, Room 2B42, Bethesda, MD 20892-7502, USA
J Immunol 169:6036-47. 2002..These observations provide an impetus to develop strategies directed toward generating HLA class II-restricted tumor-reactive T cells...
A simplified method for the clinical-scale generation of central memory-like CD8+ T cells after transduction with lentiviral vectors encoding antitumor antigen T-cell receptorsShicheng Yang
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunother 33:648-58. 2010..The methodology described here could be readily applied for engineering CD8+ T cells with antitumor specificity for human adoptive immunotherapy...
Persistence of multiple tumor-specific T-cell clones is associated with complete tumor regression in a melanoma patient receiving adoptive cell transfer therapyJuhua Zhou
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunother (1997) 28:53-62. 2005....
Primary human lymphocytes transduced with NY-ESO-1 antigen-specific TCR genes recognize and kill diverse human tumor cell linesYangbing Zhao
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 174:4415-23. 2005..These results strongly support the idea that redirection of normal T cell specificity by TCR gene transfer can have potential applications in tumor adoptive immunotherapy...
Modulation by IL-2 of CD70 and CD27 expression on CD8+ T cells: importance for the therapeutic effectiveness of cell transfer immunotherapyJianping Huang
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 176:7726-35. 2006..004) with the ability of these TILs to mediate tumor regression following adoptive transfer...
Evaluation of prime/boost regimens using recombinant poxvirus/tyrosinase vaccines for the treatment of patients with metastatic melanomaKimberly R Lindsey
Surgery Branch, National Cancer Institute, Center for Cancer Research, NIH, Bethesda, Maryland, USA
Clin Cancer Res 12:2526-37. 2006....
Treatment of metastatic melanoma using interleukin-2 alone or in conjunction with vaccinesFranz O Smith
Surgery Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 1201, USA
Clin Cancer Res 14:5610-8. 2008..v. interleukin-2 (IL-2) given either alone or in combination with a variety of melanoma vaccines...
Increased intensity lymphodepletion enhances tumor treatment efficacy of adoptively transferred tumor-specific T cellsClaudia Wrzesinski
Surgery Branch, National Cancer Institute, National Institute of Health, Bethesda, MD 20892, USA
J Immunother 33:1-7. 2010..Thus, increased intensity lymphodepletion triggers enhanced tumor treatment efficacy and the benefits of high-dose total body irradiation must be titrated against its risks...
A herceptin-based chimeric antigen receptor with modified signaling domains leads to enhanced survival of transduced T lymphocytes and antitumor activityYangbing Zhao
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 183:5563-74. 2009..More importantly, PBLs expressing this new version of the 4D5 CAR could not only efficiently lyse the autologous fresh tumor digests, but they could strongly suppress tumor growth in a xenogenic mouse model...
IL-12 triggers a programmatic change in dysfunctional myeloid-derived cells within mouse tumorsSid P Kerkar
Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 1502, USA
J Clin Invest 121:4746-57. 2011....
Adoptive transfer of vaccine-induced peripheral blood mononuclear cells to patients with metastatic melanoma following lymphodepletionDaniel J Powell
Surgery Branch, National Cancer Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
J Immunol 177:6527-39. 2006..These studies demonstrate the feasibility and safety of using vaccine-induced PBMC for cell transfer, but suggests that they are not as effective as TIL in adoptive immunotherapy even when transferred into lymphodepleted hosts...
Cancer regression and autoimmunity in patients after clonal repopulation with antitumor lymphocytesMark E Dudley
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20902, USA
Science 298:850-4. 2002..This approach presents new possibilities for the treatment of patients with cancer as well as patients with human immunodeficiency virus-related acquired immunodeficiency syndrome and other infectious diseases...
Intralymphatic dendritic cell vaccination induces tumor antigen-specific, skin-homing T lymphocytesAmelia Grover
Surgery Branch and Dermatology Branch, National Cancer Institute/NIH, 10 Center Drive, Bethesda, MD 20892, USA
Clin Cancer Res 12:5801-8. 2006....
Tumor-specific CD8+ T cells expressing interleukin-12 eradicate established cancers in lymphodepleted hostsSid P Kerkar
Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, USA
Cancer Res 70:6725-34. 2010..Our findings reveal an approach to genetically modify T cells to reduce the cell number needed, eliminate the need for vaccines or systemic IL-2, and improve immunotherapy efficacy based on adoptive transfer of gene-engineered T cells...
Central memory self/tumor-reactive CD8+ T cells confer superior antitumor immunity compared with effector memory T cellsChristopher A Klebanoff
Howard Hughes Medical Institute, National Institutes of Health Research Scholars Program, Bethesda, MD 20814, USA
Proc Natl Acad Sci U S A 102:9571-6. 2005..Thus, tumor-reactive CD8+ T cell populations with the phenotypic and functional attributes of T(CM) may be superior to T(EM)/effector T cells for adoptive immunotherapies using concomitant tumor-antigen vaccination...
Autoimmunity correlates with tumor regression in patients with metastatic melanoma treated with anti-cytotoxic T-lymphocyte antigen-4Peter Attia
Surgery Branch, National Cancer Institute, National Institutes of Health, CRC, Room 3W-3940, 10 Center Dr, Bethesda, MD 20892-1201, USA
J Clin Oncol 23:6043-53. 2005..CONCLUSION: Administration of anti-CTLA-4 monoclonal antibody plus peptide vaccination can cause durable objective responses, which correlate with the induction of autoimmunity, in patients with metastatic melanoma...
Adoptive cell therapy for patients with melanoma, using tumor-infiltrating lymphocytes genetically engineered to secrete interleukin-2Bianca Heemskerk
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Hum Gene Ther 19:496-510. 2008..In this study, insertion of the IL-2 gene into antitumor TILs increased their ability to survive after IL-2 withdrawal in vitro but did not increase their in vivo persistence or clinical effectiveness...
Enterocolitis in patients with cancer after antibody blockade of cytotoxic T-lymphocyte-associated antigen 4Kimberly E Beck
Surgery Branch and Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1201, USA
J Clin Oncol 24:2283-9. 2006..0065 for MM and P = .0016 for RCC). CONCLUSION: CTLA4 seems to be a significant component of tolerance to tumor and in protection against immune mediated enterocolitis and these phenomena are significantly associated in cancer patients...
Tumor progression can occur despite the induction of very high levels of self/tumor antigen-specific CD8+ T cells in patients with melanomaSteven A Rosenberg
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 175:6169-76. 2005..Further, the induction of even high levels of antitumor T cells may be insufficient to alter tumor progression...
Tumor regression and autoimmunity in patients treated with cytotoxic T lymphocyte-associated antigen 4 blockade and interleukin 2: a phase I/II studyAjay V Maker
Surgery Branch, National Cancer Institute, National Institutes of Health, CRC Room 3-3940, 10 Center Drive, MSC 1201, Bethesda, MD 20814, USA
Ann Surg Oncol 12:1005-16. 2005..Durable cancer regressions were seen in patients treated with this combination...
Induction of CD4+ Th1 lymphocytes that recognize known and novel class II MHC restricted epitopes from the melanoma antigen gp100 by stimulation with recombinant proteinMaria R Parkhurst
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 1502, USA
J Immunother 27:79-91. 2004..These results suggest that recombinant tumor-associated proteins may be clinically applicable for the generation of CD4+ T helper cells in active vaccination strategies or adoptive cellular immunotherapies...
Intrapatient dose escalation of anti-CTLA-4 antibody in patients with metastatic melanomaAjay V Maker
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20814, USA
J Immunother (1997) 29:455-63. 2006....
Recognition of NY-ESO-1+ tumor cells by engineered lymphocytes is enhanced by improved vector design and epigenetic modulation of tumor antigen expressionJennifer A Wargo
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Cancer Immunol Immunother 58:383-94. 2009..These results have relevance for TCR-based gene therapies targeting common epithelial malignancies...
Extrathymic generation of tumor-specific T cells from genetically engineered human hematopoietic stem cells via Notch signalingYangbing Zhao
Surgery Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 1201, USA
Cancer Res 67:2425-9. 2007..The genetic manipulation of HSCs has broad implications for ACT of cancer...
Cytokine-independent growth and clonal expansion of a primary human CD8+ T-cell clone following retroviral transduction with the IL-15 geneCary Hsu
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Blood 109:5168-77. 2007..It does not appear that the retroviral vector integration sites played a role in the continuous growth of this cell clone, but this remains under investigation...
Adoptive transfer of allogeneic tumor-specific T cells mediates effective regression of large tumors across major histocompatibility barriersAndrea Boni
Clinical Research Center, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Blood 112:4746-54. 2008..Taken together, these data indicate that the use of tumor-specific allogeneic CD8(+) T cells or CD4(+) can result in significant antitumor effects in the absence of measurable GVHD...
Construction and preclinical evaluation of an anti-CD19 chimeric antigen receptorJames N Kochenderfer
Surgery Branch of the National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunother 32:689-702. 2009....
CTLA-4 blockade with ipilimumab: long-term follow-up of 177 patients with metastatic melanomaPeter A Prieto
Surgery Branch, National Cancer Institute, NIH, Bldg 10 CRC, Room 3 5760, 10 Center Drive, Bethesda, MD 20892, USA
Clin Cancer Res 18:2039-47. 2012..Treatment with ipilimumab can cause objective tumor responses in patients with metastatic melanoma. We have treated 177 evaluable patients in three clinical trials and have long-term follow-up to evaluate the durability of responses...
Effective tumor treatment targeting a melanoma/melanocyte-associated antigen triggers severe ocular autoimmunityDouglas C Palmer
National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 105:8061-6. 2008..These findings have particular importance for immunotherapies directed against self-antigens and highlight the need for targeting unique tumor antigens not expressed in critical tissues...
Tumor antigen-specific CD8 T cells infiltrating the tumor express high levels of PD-1 and are functionally impairedMojgan Ahmadzadeh
Surgery Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
Blood 114:1537-44. 2009..These findings suggest that the tumor microenvironment can lead to up-regulation of PD-1 on tumor-reactive T cells and contribute to impaired antitumor immune responses...
Removal of homeostatic cytokine sinks by lymphodepletion enhances the efficacy of adoptively transferred tumor-specific CD8+ T cellsLuca Gattinoni
Center for Cancer Research, National Cancer Institute (NCI, National Institutes of Health (NIH, Bethesda, MD 20892, USA
J Exp Med 202:907-12. 2005..Thus, the restricted availability of homeostatic cytokines can be a contributing factor to peripheral tolerance, as well as a limiting resource for the effectiveness of tumor-specific T cells...
Gene therapy using genetically modified lymphocytes targeting VEGFR-2 inhibits the growth of vascularized syngenic tumors in miceDhanalakshmi Chinnasamy
Surgery Branch, National Cancer Institute, Clinical Research Center, Bethesda, Maryland 20892, USA
J Clin Invest 120:3953-68. 2010..T cells transduced with VEGFR-2 CAR showed durable and increased tumor infiltration, correlating with their antitumor effect. This approach provides a potential method for the gene therapy of a variety of human cancers...
Large-scale depletion of CD25+ regulatory T cells from patient leukapheresis samplesDaniel J Powell
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-1201, USA
J Immunother (1997) 28:403-11. 2005....
Adoptively transferred effector cells derived from naive rather than central memory CD8+ T cells mediate superior antitumor immunityChristian S Hinrichs
National Cancer Institute, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 106:17469-74. 2009..These results indicate that insertion of genes that confer antitumor specificity into naïve rather than central memory CD8(+) T cells may allow superior efficacy upon adoptive transfer...
Bcl-2 overexpression enhances tumor-specific T-cell survivalJehad Charo
Surgery Branch, National Cancer Institute, Bethesda, Maryland 20892, USA
Cancer Res 65:2001-8. 2005..Our data suggest that adoptive immunotherapy approaches to the treatment of cancer patients may be enhanced using Bcl-2-modified tumor-reactive T cells...
Adoptive cell transfer therapy following non-myeloablative but lymphodepleting chemotherapy for the treatment of patients with refractory metastatic melanomaMark E Dudley
Surgery Branch, National Cancer Institute, NIH, CRC 3-3940, 10 Center Dr MSC 1201, Bethesda MD 20892-1202, USA
J Clin Oncol 23:2346-57. 2005..CONCLUSION: Lymphodepleting chemotherapy followed by the transfer of highly avid antitumor lymphocytes can mediate significant tumor regression in heavily pretreated patients with IL-2 refractory metastatic melanoma...
IL-15 enhances the in vivo antitumor activity of tumor-reactive CD8+ T cellsChristopher A Klebanoff
Howard Hughes Medical Institute National Institutes of Health Research Scholars Program, Bethesda, MD 20814, USA
Proc Natl Acad Sci U S A 101:1969-74. 2004..These results provide several avenues for improving adoptive immunotherapy of cancer in patients...
Case report of a serious adverse event following the administration of T cells transduced with a chimeric antigen receptor recognizing ERBB2Richard A Morgan
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Ther 18:843-51. 2010..We speculate that the large number of administered cells localized to the lung immediately following infusion and were triggered to release cytokine by the recognition of low levels of ERBB2 on lung epithelial cells...
Cytotoxic T-lymphocyte-associated antigen-4 blockage can induce autoimmune hypophysitis in patients with metastatic melanoma and renal cancerJoseph A Blansfield
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-1201, USA
J Immunother (1997) 28:593-8. 2005..The characteristics, clinical course, laboratory values, radiographic findings, and treatment of these 8 patients are the focus of this report...
TGF-beta 1 attenuates the acquisition and expression of effector function by tumor antigen-specific human memory CD8 T cellsMojgan Ahmadzadeh
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 174:5215-23. 2005....
Tumor regression in patients with metastatic synovial cell sarcoma and melanoma using genetically engineered lymphocytes reactive with NY-ESO-1Paul F Robbins
National Institutes of Health, National Cancer Institute, Surgery Branch, Bethesda, MD 20892 1201, USA
J Clin Oncol 29:917-24. 2011....
Acquisition of full effector function in vitro paradoxically impairs the in vivo antitumor efficacy of adoptively transferred CD8+ T cellsLuca Gattinoni
Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892-1502, USA
J Clin Invest 115:1616-26. 2005..These findings suggest that the current methodology for selecting T cells for transfer is inadequate and provide new criteria for the generation and the screening of optimal lymphocyte populations for adoptive immunotherapy...
Selective growth, in vitro and in vivo, of individual T cell clones from tumor-infiltrating lymphocytes obtained from patients with melanomaJuhua Zhou
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 173:7622-9. 2004..A similar analysis may also be useful for monitoring autoimmune responses...
Tumor regression and autoimmunity after reversal of a functionally tolerant state of self-reactive CD8+ T cellsWillem W Overwijk
National Cancer Institute (NCI, National Institute of Health, Bethesda, MD 20892-1502, USA
J Exp Med 198:569-80. 2003....
Pre-existing immunity to tyrosinase-related protein (TRP)-2, a new TRP-2 isoform, and the NY-ESO-1 melanoma antigen in a patient with a dramatic response to immunotherapyHung T Khong
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 168:951-6. 2002....
Normal tissue depresses while tumor tissue enhances human T cell responses in vivo to a novel self/tumor melanoma antigen, OA1Christopher E Touloukian
National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 170:1579-85. 2003..Thus, the tumor-bearing state reverses, in part, the tolerance of T cells that results from the normal expression of tissue differentiation Ags...
Identification of multiple antigens recognized by tumor-infiltrating lymphocytes from a single patient: tumor escape by antigen loss and loss of MHC expressionHung T Khong
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-1502, USA
J Immunother (1997) 27:184-90. 2004..This paper thus provides evidence for both the effectiveness of the immune destruction of cancer as well as problems associated with antigen-loss tumor escape mechanisms...
Primary human T lymphocytes engineered with a codon-optimized IL-15 gene resist cytokine withdrawal-induced apoptosis and persist long-term in the absence of exogenous cytokineCary Hsu
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 175:7226-34. 2005..In the setting of adoptive cell transfer, IL-15-transduced lymphocytes may prolong lymphocyte survival in vivo and could potentially enhance antitumor activity...
Frequency of MART-1/MelanA and gp100/PMel17-specific T cells in tumor metastases and cultured tumor-infiltrating lymphocytesSimone Seiter
Department of Transfusion Medicine, Center for Cancer Research, National Cancer Institute, Building 10, Room 2B42, 9000 Rockville Pike, Bethesda, MD 20892, USA
J Immunother (1997) 25:252-63. 2002..These data provide direct enumeration of MART-1/MelanA and gp100/pMel17 reactivity ex vivo and in vitro in the context of HLA-A*0201...
Phenotypic and functional maturation of tumor antigen-reactive CD8+ T lymphocytes in patients undergoing multiple course peptide vaccinationDaniel J Powell
Surgery Branch, National Cancer Institutes of Health, Bethesda, MD 20892, USA
J Immunother (1997) 27:36-47. 2004....
Ocular and systemic autoimmunity after successful tumor-infiltrating lymphocyte immunotherapy for recurrent, metastatic melanomaSteven Yeh
National Eye Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20814, USA
Ophthalmology 116:981-989.e1. 2009..To describe the ophthalmic and systemic autoimmune findings after successful adoptive cell transfer of ex vivo expanded, autologous tumor-reactive tumor-infiltrating lymphocytes (TIL) for metastatic melanoma...
IL-2 and IL-15 each mediate de novo induction of FOXP3 expression in human tumor antigen-specific CD8 T cellsMojgan Ahmadzadeh
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunother 30:294-302. 2007....
Inability of a fusion protein of IL-2 and diphtheria toxin (Denileukin Diftitox, DAB389IL-2, ONTAK) to eliminate regulatory T lymphocytes in patients with melanomaPeter Attia
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-1201, USA
J Immunother (1997) 28:582-92. 2005..Administration of Denileukin Diftitox does not appear to eliminate regulatory T lymphocytes or cause regression of metastatic melanoma...
Gene transfer of tumor-reactive TCR confers both high avidity and tumor reactivity to nonreactive peripheral blood mononuclear cells and tumor-infiltrating lymphocytesLaura A Johnson
Surgery Branch, National Cancer Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
J Immunol 177:6548-59. 2006..We propose that inducing expression of this highly avid TCR in patient PBMC has the potential to induce tumor regression, as an "off-the-shelf" reagent for allogeneic melanoma patient gene therapy...
Immunization of HLA-A*0201 and/or HLA-DPbeta1*04 patients with metastatic melanoma using epitopes from the NY-ESO-1 antigenHung T Khong
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-1502, USA
J Immunother (1997) 27:472-7. 2004..This result raises questions about the use of synthetic peptides derived from NY-ESO-1 as a sole form of immunization...
Recognition of fresh human tumor by human peripheral blood lymphocytes transduced with a bicistronic retroviral vector encoding a murine anti-p53 TCRCyrille J Cohen
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 175:5799-808. 2005..These results suggest that lymphocytes genetically engineered to express anti-p53 TCR may be of value for the adoptive immunotherapy of patients with a variety of common malignancies...
Stimulation of tumor-reactive T lymphocytes using mixtures of synthetic peptides derived from tumor-associated antigens with diverse MHC binding affinitiesJohn P Riley
Surgery Branch, National Cancer Institute, National Institutes of Health, Room 2B42, Building 10, 9000 Rockville Pike, Bethesda, MD 20892-1502, USA
J Immunol Methods 276:103-19. 2003..These results suggest that the use of peptide mixtures may facilitate the identification of new tumor-associated antigens through the application of reverse immunology...
Clinical-scale lentiviral vector transduction of PBL for TCR gene therapy and potential for expression in less-differentiated cellsShicheng Yang
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
J Immunother 31:830-9. 2008..Compared with gamma-retroviral vectors, the TCR transgene could be preferentially expressed on a less-differentiated cell population...
T cells associated with tumor regression recognize frameshifted products of the CDKN2A tumor suppressor gene locus and a mutated HLA class I gene productJianping Huang
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 172:6057-64. 2004....
Partial reduction of human FOXP3+ CD4 T cells in vivo after CD25-directed recombinant immunotoxin administrationDaniel J Powell
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
J Immunother 31:189-98. 2008....
Expression of a "self-"antigen by human tumor cells enhances tumor antigen-specific CD4(+) T-cell functionChristopher E Touloukian
National Cancer Institute, NIH, Building 10, Room 2B42, Bethesda, MD 20892, USA
Cancer Res 62:5144-7. 2002..These findings indicate that antigens expressed by malignant melanoma cells can partially activate CD4(+) T lymphocytes...
CD4+CD25+ suppressor lymphocytes in the circulation of patients immunized against melanoma antigensLuv Ram Javia
Howard Hughes Medical Institute-National Institutes of Health Research Scholars Program, Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, U.S.A
J Immunother (1997) 26:85-93. 2003....
Impact of a recombinant fowlpox vaccine on the efficacy of adoptive cell therapy with tumor infiltrating lymphocytes in a patient with metastatic melanomaFranz O Smith
Surgery Branch, National Cancer Institute, National Institute of Health, Bethesda, MD 20892 1201, USA
J Immunother 32:870-4. 2009..Cell proliferation in vitro was further stimulated by additional vaccine and interleukin-2. The patient has an ongoing partial response at 10 months after the last treatment...
Selective elimination of human regulatory T lymphocytes in vitro with the recombinant immunotoxin LMB-2Peter Attia
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunother (1997) 29:208-14. 2006..The short in vivo half-life of LMB-2 makes it an attractive candidate for reducing human T(reg) cells in vivo before the administration of cancer vaccine or cell transfer immunotherapy approaches...
Transduction of an HLA-DP4-restricted NY-ESO-1-specific TCR into primary human CD4+ lymphocytesYangbing Zhao
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
J Immunother (1997) 29:398-406. 2006..Major histocompatibility complex class II TCR-transduced CD4 T cells provides an alternative source of tumor antigen-specific T cells for adoptive immunotherapy of cancer patients...
Glucocorticoids do not inhibit antitumor activity of activated CD8+ T cellsChristian S Hinrichs
National Cancer Institute (NCI, National Institutes of Health (NIH, Bethesda, Maryland 20892-1502, USA
J Immunother (1997) 28:517-24. 2005..Finally, because glucocorticoids had no effect on tumor regression in this model, it may be possible to use glucocorticoids in some patients receiving ACT-based immunotherapy regimens...
Identification of endogenous HLA-A2-restricted reactivity against shared melanoma antigens in patients using the quantitative real-time polymerase chain reactionStacy E Thurber
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
J Immunother (1997) 25:63-71. 2002....
High-efficiency transfection of primary human and mouse T lymphocytes using RNA electroporationYangbing Zhao
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Mol Ther 13:151-9. 2006..The results of this study indicate that mRNA electroporation provides a powerful tool to introduce genes into both human and murine primary T lymphocytes...
Survival, persistence, and progressive differentiation of adoptively transferred tumor-reactive T cells associated with tumor regressionJianping Huang
Surgery Branch, National Cancer Institute, National Institute of Health, Bethesda, Maryland 20892, USA
J Immunother (1997) 28:258-67. 2005....
Lack of specific gamma-retroviral vector long terminal repeat promoter silencing in patients receiving genetically engineered lymphocytes and activation upon lymphocyte restimulationWilliam R Burns
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Blood 114:2888-99. 2009..The use of immunomodulatory adjuvants, eg, vaccination or cytokine therapy, for in vivo T-cell activation may help overcome this metabolic quiescence and thus augment cellular immunotherapy-based cancer therapy...
Inability to immunize patients with metastatic melanoma using plasmid DNA encoding the gp100 melanoma-melanocyte antigenSteven A Rosenberg
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 1502, USA
Hum Gene Ther 14:709-14. 2003..We were thus unable to demonstrate significant clinical or immunologic responses to plasmid DNA encoding the "self" nonmutated gp100 tumor antigen...
A randomized trial of bevacizumab, an anti-vascular endothelial growth factor antibody, for metastatic renal cancerJames C Yang
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
N Engl J Med 349:427-34. 2003..We conducted a clinical trial to evaluate bevacizumab, a neutralizing antibody against vascular endothelial growth factor, in patients with metastatic renal-cell carcinoma...
Generation of tumor-infiltrating lymphocyte cultures for use in adoptive transfer therapy for melanoma patientsMark E Dudley
Surgery Branch, National Cancer Institute, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892 1502, USA
J Immunother 26:332-42. 2003..These methods were efficient at generating TILs suitable for adoptive transfer therapy...
Interleukin-2-independent proliferation of human melanoma-reactive T lymphocytes transduced with an exogenous IL-2 gene is stimulation dependentKe Liu
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
J Immunother 26:190-201. 2003....
Hybrids of dendritic cells and tumor cells generated by electrofusion simultaneously present immunodominant epitopes from multiple human tumor-associated antigens in the context of MHC class I and class II moleculesMaria R Parkhurst
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 170:5317-25. 2003....
Altered CD8(+) T-cell responses when immunizing with multiepitope peptide vaccinesSteven A Rosenberg
Surgery Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
J Immunother 29:224-31. 2006....
Identification of BING-4 cancer antigen translated from an alternative open reading frame of a gene in the extended MHC class II region using lymphocytes from a patient with a durable complete regression following immunotherapySteven A Rosenberg
Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 10, Room 2B42, Bethesda, MD 20892, USA
J Immunol 168:2402-7. 2002..Overexpression was not found in normal tissues or other tumor types. Thus, BING-4 represents another candidate Ag for possible use in the immunotherapy of patients with melanoma...
A phase I study of nonmyeloablative chemotherapy and adoptive transfer of autologous tumor antigen-specific T lymphocytes in patients with metastatic melanomaMark E Dudley
Surgery Branch, National Cancer Institute, Building 10, Room 2B08, 9000 Rockville Pike, Bethesda, MD 20892, USA
J Immunother 25:243-51. 2002..This study established a nonmyeloablative-conditioning regimen that could be safely administered in conjunction with adoptive T-cell transfer and IL-2 in patients with metastatic melanoma...
Telomere length of transferred lymphocytes correlates with in vivo persistence and tumor regression in melanoma patients receiving cell transfer therapyJuhua Zhou
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 175:7046-52. 2005....
