H F Rosenberg


Affiliation: National Institutes of Health
Country: USA


  1. Dyer K, Drummond R, Rice T, Percopo C, Brenner T, Barisas D, et al. Priming of the Respiratory Tract with Immunobiotic Lactobacillus plantarum Limits Infection of Alveolar Macrophages with Recombinant Pneumonia Virus of Mice (rK2-PVM). J Virol. 2016;90:979-91 pubmed publisher
    ..This is the first study to provide insight into the cellular basis of the antiviral impact of immunobiotic L. plantarum. ..
  2. Rosenberg H. Eosinophil-Derived Neurotoxin (EDN/RNase 2) and the Mouse Eosinophil-Associated RNases (mEars): Expanding Roles in Promoting Host Defense. Int J Mol Sci. 2015;16:15442-55 pubmed publisher
    ..This review provides an update on recent discoveries, and highlights the versatility of this family in promoting innate immunity. ..
  3. Percopo C, Rice T, Brenner T, Dyer K, Luo J, Kanakabandi K, et al. Immunobiotic Lactobacillus administered post-exposure averts the lethal sequelae of respiratory virus infection. Antiviral Res. 2015;121:109-19 pubmed publisher
    ..Taken together, L. plantarum is a versatile and flexible agent that is capable of averting the lethal sequelae of severe respiratory infection both prior to and post-virus challenge via complex and potentially redundant mechanisms. ..
  4. Rosenberg H, Dyer K, Domachowske J. Respiratory viruses and eosinophils: exploring the connections. Antiviral Res. 2009;83:1-9 pubmed publisher
    ..Lastly, we consider the data that focus on the role of eosinophils in promoting virus clearance and antiviral host defense, and conclude with a recent study that explores the role of eosinophils themselves as targets of virus infection. ..
  5. Geslewitz W, Percopo C, Rosenberg H. FACS isolation of live mouse eosinophils at high purity via a protocol that does not target Siglec F. J Immunol Methods. 2018;454:27-31 pubmed publisher
    ..Eosinophils isolated by this method (yield, ~4×106/mouse) generated high-quality RNA suitable for gene expression analysis. ..
  6. Ma M, Rice T, Percopo C, Rosenberg H. Silkworm larvae plasma (SLP) assay for detection of bacteria: False positives secondary to inflammation in vivo. J Microbiol Methods. 2017;132:9-13 pubmed publisher
    ..Here, we present results that indicate that generation of melanin by this assay is not fully reliable as a surrogate marker for bacterial count. ..
  7. Kraemer L, Brenner T, Krumholz J, Rosenberg H. A flow-cytometric method to evaluate eosinophil-mediated uptake of probiotic Lactobacillus reuteri. J Microbiol Methods. 2017;137:19-24 pubmed publisher
    ..The assay may be adapted for use in vivo. ..
  8. Bonville C, Percopo C, Dyer K, Gao J, Prussin C, Foster B, et al. Interferon-gamma coordinates CCL3-mediated neutrophil recruitment in vivo. BMC Immunol. 2009;10:14 pubmed publisher
  9. Chan C, Moser J, Dyer K, Percopo C, Rosenberg H. Genetic diversity of human RNase 8. BMC Genomics. 2012;13:40 pubmed publisher

More Information


  1. Rosenberg H, Dyer K, Foster P. Eosinophils: changing perspectives in health and disease. Nat Rev Immunol. 2013;13:9-22 pubmed publisher
  2. Yamada K, Barker T, Dyer K, Rice T, Percopo C, Garcia Crespo K, et al. Eosinophil-associated ribonuclease 11 is a macrophage chemoattractant. J Biol Chem. 2015;290:8863-75 pubmed publisher
    ..Taken together, this work characterizes a divergent RNase A ribonuclease with a unique expression pattern and function, and highlights the versatility of this family in promoting innate immunity. ..
  3. Rosenberg H, Domachowske J. Inflammatory responses to respiratory syncytial virus (RSV) infection and the development of immunomodulatory pharmacotherapeutics. Curr Med Chem. 2012;19:1424-31 pubmed
    ..From this perspective, we consider the case for specific immunomodulatory strategies that may have the potential to alleviate some of the more serious sequelae of this disease. ..
  4. Percopo C, Dyer K, Garcia Crespo K, Gabryszewski S, Shaffer A, Domachowske J, et al. B cells are not essential for Lactobacillus-mediated protection against lethal pneumovirus infection. J Immunol. 2014;192:5265-72 pubmed publisher
    ..We conclude that B cells are dispensable for Lactobacillus-mediated heterologous immunity and were not crucial for promoting survival in response to an otherwise lethal pneumovirus infection. ..
  5. Rosenberg H, Dyer K, Domachowske J. Eosinophils and their interactions with respiratory virus pathogens. Immunol Res. 2009;43:128-37 pubmed publisher
    ..Interestingly, PVM replicates in eosinophils and promotes cytokine release. The molecular basis of virus infection in eosinophils and its relationship to disease outcome is currently under study. ..
  6. Rosenberg H, Domachowske J. Pneumonia virus of mice: severe respiratory infection in a natural host. Immunol Lett. 2008;118:6-12 pubmed publisher
  7. Brenner T, Rice T, Anderson E, Percopo C, Rosenberg H. Immortalized MH-S cells lack defining features of primary alveolar macrophages and do not support mouse pneumovirus replication. Immunol Lett. 2016;172:106-12 pubmed publisher
    ..However, MH-S cells do not express the GM-CSF receptor alpha chain (CD116) and do not respond to GM-CSF. Due to these unusual features, MH-S cells should be used with caution as experimental models of AMs. ..
  8. Rice T, Brenner T, Percopo C, Ma M, Keicher J, Domachowske J, et al. Signaling via pattern recognition receptors NOD2 and TLR2 contributes to immunomodulatory control of lethal pneumovirus infection. Antiviral Res. 2016;132:131-40 pubmed publisher
  9. Percopo C, Brenner T, Ma M, Kraemer L, Hakeem R, Lee J, et al. SiglecF+Gr1hi eosinophils are a distinct subpopulation within the lungs of allergen-challenged mice. J Leukoc Biol. 2017;101:321-328 pubmed publisher
    ..These cytokines may facilitate eosinophil-mediated immunomodulatory responses in the allergen-challenged lung as well as in other distinct microenvironments. ..
  10. request reprint
    Rosenberg H, Masterson J, Furuta G. Eosinophils, probiotics, and the microbiome. J Leukoc Biol. 2016;100:881-888 pubmed
    ..Likewise, although is it clear that eosinophils can and do interact with bacteria in vivo, their ability to discern between pathogenic and probiotic species in various settings remains to be explored. ..
  11. Percopo C, Ma M, Rosenberg H. Administration of immunobiotic Lactobacillus plantarum delays but does not prevent lethal pneumovirus infection in Rag1-/- mice. J Leukoc Biol. 2017;102:905-913 pubmed publisher
    ..Our results suggest that a larger understanding of the mechanisms and mediators that limit acute virus-induced inflammation may yield new and useful therapeutic modalities. ..
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    Rosenberg H, Bonville C, Easton A, Domachowske J. The pneumonia virus of mice infection model for severe respiratory syncytial virus infection: identifying novel targets for therapeutic intervention. Pharmacol Ther. 2005;105:1-6 pubmed
    ..Ongoing exploration into the biology of PVM infection will identify other pathways and targets to be exploited for immunomodulatory control of hRSV and related severe respiratory virus infections...