Mario Roederer

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint How many events is enough? Are you positive?
    Mario Roederer
    Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA
    Cytometry A 73:384-5. 2008
  2. ncbi request reprint Intracellular cytokine optimization and standard operating procedure
    Laurie Lamoreaux
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Protoc 1:1507-16. 2006
  3. ncbi request reprint Live-cell assay to detect antigen-specific CD4+ T-cell responses by CD154 expression
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Protoc 1:1-6. 2006
  4. ncbi request reprint Quality assurance for polychromatic flow cytometry
    Stephen P Perfetto
    Flow Cytometry Core Facility, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Protoc 1:1522-30. 2006
  5. pmc Immunisation with BCG and recombinant MVA85A induces long-lasting, polyfunctional Mycobacterium tuberculosis-specific CD4+ memory T lymphocyte populations
    Natalie E R Beveridge
    Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Medicine, University of Oxford, Oxford, UK
    Eur J Immunol 37:3089-100. 2007
  6. pmc Vaccination preserves CD4 memory T cells during acute simian immunodeficiency virus challenge
    Joseph J Mattapallil
    Vaccine Research Center, National Institute of Allergy and Infectious Disease NIAID, National Institutes of Health NIH, Bethesda, MD 20892, USA
    J Exp Med 203:1533-41. 2006
  7. pmc Standardization of cytokine flow cytometry assays
    Holden T Maecker
    BD Biosciences, San Jose, USA
    BMC Immunol 6:13. 2005
  8. pmc Polychromatic plots: graphical display of multidimensional data
    Mario Roederer
    Vaccine Research Center, NIH, Bethesda, Maryland 20892 3015, USA
    Cytometry A 73:868-74. 2008
  9. ncbi request reprint Flow cytometric analysis of vaccine responses: how many colors are enough?
    Mario Roederer
    Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA
    Clin Immunol 110:199-205. 2004
  10. doi request reprint Interpretation of cellular proliferation data: Avoid the panglossian
    Mario Roederer
    Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland
    Cytometry A 79:95-101. 2011

Detail Information

Publications110 found, 100 shown here

  1. ncbi request reprint How many events is enough? Are you positive?
    Mario Roederer
    Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA
    Cytometry A 73:384-5. 2008
  2. ncbi request reprint Intracellular cytokine optimization and standard operating procedure
    Laurie Lamoreaux
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Protoc 1:1507-16. 2006
    ....
  3. ncbi request reprint Live-cell assay to detect antigen-specific CD4+ T-cell responses by CD154 expression
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Protoc 1:1-6. 2006
    ..Unlike other assays, this method allows simultaneous assessment of other cell phenotypes or functions, is compatible with downstream RNA-based assays and preserves cell viability. This protocol can be completed in 9 h...
  4. ncbi request reprint Quality assurance for polychromatic flow cytometry
    Stephen P Perfetto
    Flow Cytometry Core Facility, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Protoc 1:1522-30. 2006
    ..All three aspects of this program should be performed upon installation, or whenever changes occur along the flow cytometer's optical path. However, only a few of these procedures need to be carried out on a routine basis...
  5. pmc Immunisation with BCG and recombinant MVA85A induces long-lasting, polyfunctional Mycobacterium tuberculosis-specific CD4+ memory T lymphocyte populations
    Natalie E R Beveridge
    Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Medicine, University of Oxford, Oxford, UK
    Eur J Immunol 37:3089-100. 2007
    ..Overall, these data strongly support the use of MVA85A in humans as a boosting agent to expand polyfunctional M.tb-specific CD4(+) T cells capable of significant secondary responses...
  6. pmc Vaccination preserves CD4 memory T cells during acute simian immunodeficiency virus challenge
    Joseph J Mattapallil
    Vaccine Research Center, National Institute of Allergy and Infectious Disease NIAID, National Institutes of Health NIH, Bethesda, MD 20892, USA
    J Exp Med 203:1533-41. 2006
    ....
  7. pmc Standardization of cytokine flow cytometry assays
    Holden T Maecker
    BD Biosciences, San Jose, USA
    BMC Immunol 6:13. 2005
    ....
  8. pmc Polychromatic plots: graphical display of multidimensional data
    Mario Roederer
    Vaccine Research Center, NIH, Bethesda, Maryland 20892 3015, USA
    Cytometry A 73:868-74. 2008
    ..In many ways, the display performs somewhat like an unsupervised cluster algorithm, by highlighting events of similar distributions in multivariate space...
  9. ncbi request reprint Flow cytometric analysis of vaccine responses: how many colors are enough?
    Mario Roederer
    Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA
    Clin Immunol 110:199-205. 2004
    ..In this manuscript, we discuss these technologies, with a focus on assisting in the design and implementation of immunogenicity trials for future vaccine efforts...
  10. doi request reprint Interpretation of cellular proliferation data: Avoid the panglossian
    Mario Roederer
    Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland
    Cytometry A 79:95-101. 2011
    ..Published 2011 Wiley-Liss, Inc...
  11. pmc SPICE: exploration and analysis of post-cytometric complex multivariate datasets
    Mario Roederer
    Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA
    Cytometry A 79:167-74. 2011
    ..While originally developed to support the analysis of T cell functional profiles, these techniques are amenable to a broad range of datatypes...
  12. ncbi request reprint Optimized determination of T cell epitope responses
    Mario Roederer
    Vaccine Research Center, NIAID, NIH, 40 Convent Drive, Room 5509, Bethesda, MD 20892, USA
    J Immunol Methods 274:221-8. 2003
    ..In addition, our results guide the design and implementation of the experiments to deconvolute the responses to individual peptide epitopes...
  13. pmc Phase 1 safety and immunogenicity evaluation of a multiclade HIV-1 DNA candidate vaccine
    Barney S Graham
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 3017, USA
    J Infect Dis 194:1650-60. 2006
    ..Vaccine Research Center (VRC) 004 is the first phase 1 dose-escalation study of a multiclade HIV-1 DNA vaccine...
  14. pmc Acquisition of direct antiviral effector functions by CMV-specific CD4+ T lymphocytes with cellular maturation
    Joseph P Casazza
    Immunology Laboratory, Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases NIAID, National Institutes of Health NIH, Bethesda, MD 20892, USA
    J Exp Med 203:2865-77. 2006
    ..Thus, mature CMV-specific CD4+ T cells exhibit distinct functional properties reminiscent of antiviral CD8+ T lymphocytes...
  15. ncbi request reprint Phase I clinical evaluation of a six-plasmid multiclade HIV-1 DNA candidate vaccine
    Andrew T Catanzaro
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Building 40, Bethesda, MD 20892 3017, USA
    Vaccine 25:4085-92. 2007
    ..Compared to a four-plasmid product, Gag- and Nef-specific T cell responses were improved, while Env-specific responses were maintained. This candidate vaccine has now advanced to Phase II evaluation...
  16. pmc Priming immunization with DNA augments immunogenicity of recombinant adenoviral vectors for both HIV-1 specific antibody and T-cell responses
    Richard A Koup
    Vaccine Research Center, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 5:e9015. 2010
    ..Prime-boost regimens using heterologous gene-based vaccine vectors have induced potent, polyfunctional T cell responses in preclinical studies...
  17. ncbi request reprint The functional profile of primary human antiviral CD8+ T cell effector activity is dictated by cognate peptide concentration
    Michael R Betts
    Immunology Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 172:6407-17. 2004
    ..The inherent ability of viruses to induce high or low Ag states may be the primary determinant of the cytokine vs cytolytic nature of the virus-specific CD8(+) T cell response...
  18. pmc Phase 1 safety and immunogenicity evaluation of a multiclade HIV-1 candidate vaccine delivered by a replication-defective recombinant adenovirus vector
    Andrew T Catanzaro
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 3017, USA
    J Infect Dis 194:1638-49. 2006
    ..Here, we report the safety, tolerability, and immunogenicity of a replication-defective recombinant adenovirus serotype 5 (rAd5) vector HIV-1 candidate vaccine...
  19. pmc SIV-specific CD8+ T cells express high levels of PD1 and cytokines but have impaired proliferative capacity in acute and chronic SIVmac251 infection
    Constantinos Petrovas
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases NIH, 40 Convent Drive, Bethesda, MD 20892, USA
    Blood 110:928-36. 2007
    ..Manipulation of the interaction of PD-1 with its ligands could thus potentially restore the CD8+ T-cell responses in SIV infection...
  20. pmc Toll-like receptor agonists influence the magnitude and quality of memory T cell responses after prime-boost immunization in nonhuman primates
    Ulrike Wille-Reece
    Cellular Immunology Section, Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 203:1249-58. 2006
    ..These data provide insights for designing prime-boost immunization regimens to optimize Th1 and CD8+ T cell responses...
  21. pmc Differential association of programmed death-1 and CD57 with ex vivo survival of CD8+ T cells in HIV infection
    Constantinos Petrovas
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20814, USA
    J Immunol 183:1120-32. 2009
    ..Thus, our data further support the role of PD-1 as a preapoptotic factor for CD8(+) T cells in HIV infection...
  22. pmc Reduced protection from simian immunodeficiency virus SIVmac251 infection afforded by memory CD8+ T cells induced by vaccination during CD4+ T-cell deficiency
    Monica Vaccari
    Animal Models and Retroviral Vaccines Section, National Cancer Institute, Bldg 41, Room D804, Bethesda, MD 20892, USA
    J Virol 82:9629-38. 2008
    ....
  23. pmc Therapeutic vaccination expands and improves the function of the HIV-specific memory T-cell repertoire
    Joseph P Casazza
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health NIH, Bethesda, Maryland 20892, USA
    J Infect Dis 207:1829-40. 2013
    ..The licensing of herpes zoster vaccine has demonstrated that therapeutic vaccination can help control chronic viral infection. Unfortunately, human trials of immunodeficiency virus (HIV) vaccine have shown only marginal efficacy...
  24. pmc Public clonotype usage identifies protective Gag-specific CD8+ T cell responses in SIV infection
    David A Price
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 206:923-36. 2009
    ..Thus, the pattern of antigen-specific clonotype recruitment within a protective CD8(+) T cell population is a prognostic indicator of vaccine efficacy and biological outcome in an AIDS virus infection...
  25. pmc High frequencies of resting CD4+ T cells containing integrated viral DNA are found in rhesus macaques during acute lentivirus infections
    Yoshiaki Nishimura
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:8015-20. 2009
    ..Prompt and sustained interventions are therefore required to block the rapid systemic dissemination of virus and prevent an otherwise fatal clinical outcome...
  26. pmc Superior T memory stem cell persistence supports long-lived T cell memory
    Enrico Lugli
    Immunotechnology Section, Vaccine Research Center, NIAID, NIH, 40, Convent Dr, Bethesda, Maryland 20892, USA
    J Clin Invest 123:594-9. 2013
    ..Thus, one mechanism for maintenance of long-term T cell memory derives from the unique homeostatic properties of TSCM cells. Vaccination strategies designed to elicit durable cellular immunity should target the generation of TSCM cells...
  27. pmc A SARS DNA vaccine induces neutralizing antibody and cellular immune responses in healthy adults in a Phase I clinical trial
    Julie E Martin
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, MSC 2610, Bethesda, MD 20892 3017, USA
    Vaccine 26:6338-43. 2008
    ..Over 8000 cases and 900 deaths occurred during the epidemic. We report the safety and immunogenicity of a SARS DNA vaccine in a Phase I human study...
  28. pmc The transfer of adaptive immunity to CMV during hematopoietic stem cell transplantation is dependent on the specificity and phenotype of CMV-specific T cells in the donor
    Phillip Scheinberg
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Blood 114:5071-80. 2009
    ....
  29. pmc Rational design of envelope identifies broadly neutralizing human monoclonal antibodies to HIV-1
    Xueling Wu
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Science 329:856-61. 2010
    ..Exceptionally broad HIV-1 neutralization can be achieved with individual antibodies targeted to the functionally conserved CD4bs of glycoprotein 120, an important insight for future HIV-1 vaccine design...
  30. pmc Replication-defective adenovirus vectors with multiple deletions do not induce measurable vector-specific T cells in human trials
    Richard A Koup
    Immunology Laboratory, Immunology Core Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 83:6318-22. 2009
    ..These data indicate that rAd5-based vaccines containing deletions in the E1, E3, and E4 regions do not induce appreciable expansion of vector-specific CD4(+) T cells...
  31. pmc T-cell subsets that harbor human immunodeficiency virus (HIV) in vivo: implications for HIV pathogenesis
    Jason M Brenchley
    Human Immunology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 78:1160-8. 2004
    ..These data illuminate the underlying mechanisms that distort T-cell homeostasis in HIV infection...
  32. pmc HIV Gag protein conjugated to a Toll-like receptor 7/8 agonist improves the magnitude and quality of Th1 and CD8+ T cell responses in nonhuman primates
    Ulrike Wille-Reece
    Cellular Immunology Section, Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:15190-4. 2005
    ..This type of vaccine formulation should have utility in preventive or therapeutic vaccines in which humoral and cellular immunity is required...
  33. ncbi request reprint Systemic vaccination prevents the total destruction of mucosal CD4 T cells during acute SIV challenge
    Joseph J Mattapallil
    Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA
    J Med Primatol 35:217-24. 2006
    ..The primary objective of this study was to determine if systemic vaccination with DNA/rAd-5 encoding SIV-mac239-env, gag and pol could prevent the destruction of CD4 T cells in mucosal tissues...
  34. pmc The cytolytic enzymes granyzme A, granzyme B, and perforin: expression patterns, cell distribution, and their relationship to cell maturity and bright CD57 expression
    Pratip K Chattopadhyay
    Immunotechnology Section, Laboratory of Immunology, Vaccine Research Center, National Institutes of Health, Bethesda, MD, USA
    J Leukoc Biol 85:88-97. 2009
    ..Thus, the use of CD57 provides a means to easily isolate viable cells with high cytolytic potential, without the need for lethal fixation/permeabilization techniques...
  35. pmc Trafficking, persistence, and activation state of adoptively transferred allogeneic and autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones during acute and chronic infection of rhesus macaques
    Diane L Bolton
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 184:303-14. 2010
    ..The lack of impact following transfer of such a large number of functional Ag-specific CD8(+) T cells on SIV replication may reflect the magnitude of the immune response required to contain the virus...
  36. pmc IL-10 production differentially influences the magnitude, quality, and protective capacity of Th1 responses depending on the vaccine platform
    Patricia A Darrah
    Cellular Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 207:1421-33. 2010
    ..Overall, we delineate distinct mechanisms by which vaccines elicit protective Th1 responses and underscore the importance of multifunctional CD4(+) T cells...
  37. pmc Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramers
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cytometry A 73:1001-9. 2008
    ....
  38. pmc Avidity for antigen shapes clonal dominance in CD8+ T cell populations specific for persistent DNA viruses
    David A Price
    Human Immunology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 202:1349-61. 2005
    ..Vaccine strategies that reconstruct these biological processes could generate T cell populations that mediate optimal delivery of antiviral effector function...
  39. pmc Immunization with vaccinia virus induces polyfunctional and phenotypically distinctive CD8(+) T cell responses
    Melissa L Precopio
    Immunology Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 204:1405-16. 2007
    ..This quality of the CD8(+) T cell response may be at least partially responsible for the profound efficacy of these vaccines in protection against smallpox and serves as a benchmark against which other vaccines can be evaluated...
  40. pmc Amine-reactive dyes for dead cell discrimination in fixed samples
    Stephen P Perfetto
    Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
    Curr Protoc Cytom . 2010
    ..This unit describes procedures, troubleshooting, and outcomes for using the two most commonly used amine-reactive dyes, ViViD and Aqua Blue...
  41. ncbi request reprint Vaccination in humans generates broad T cell cytokine responses
    Stephen C De Rosa
    Vaccine Research Center, National Institute of Allery and Infectious Diseases, NIH, Bethesda, MD 20892, USA
    J Immunol 173:5372-80. 2004
    ..The presence and variability of these complex response profiles introduce the possibility that selective functional expression patterns may provide correlates for vaccine efficacy or disease progression...
  42. pmc Autocrine production of beta-chemokines protects CMV-Specific CD4 T cells from HIV infection
    Joseph P Casazza
    Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
    PLoS Pathog 5:e1000646. 2009
    ..These data suggest that CD4+ T cells which produce MIP-1alpha and MIP-1beta bind these chemokines in an autocrine fashion which decreases the risk of in vivo HIV infection...
  43. pmc Safety and immunogenicity of a Gag-Pol candidate HIV-1 DNA vaccine administered by a needle-free device in HIV-1-seronegative subjects
    Jorge A Tavel
    Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Acquir Immune Defic Syndr 44:601-5. 2007
    ..To evaluate the safety and immunogenicity of a candidate HIV DNA vaccine administered using a needle-free device...
  44. pmc Immunological and virological mechanisms of vaccine-mediated protection against SIV and HIV
    Mario Roederer
    Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA
    Nature 505:502-8. 2014
    ..These analyses provide insight into the limited efficacy seen in HIV vaccine trials. ..
  45. pmc Differential specificity and immunogenicity of adenovirus type 5 neutralizing antibodies elicited by natural infection or immunization
    Cheng Cheng
    Vaccine Research Center, NIAID, National Institutes of Health, Bethesda, MD 20892 3005, USA
    J Virol 84:630-8. 2010
    ..These results have implications for future AIDS vaccine trials and the design of next-generation gene-based vaccine vectors...
  46. ncbi request reprint Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry
    Pratip K Chattopadhyay
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Med 12:972-7. 2006
    ....
  47. pmc HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T cells
    Michael R Betts
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 107:4781-9. 2006
    ..Thus, rather than quantity or phenotype, the quality of the CD8(+) T-cell functional response serves as an immune correlate of HIV disease progression and a potential qualifying factor for evaluation of HIV vaccine efficacy...
  48. pmc Characterization of functional and phenotypic changes in anti-Gag vaccine-induced T cell responses and their role in protection after HIV-1 infection
    Michael R Betts
    Laboratory of Immunology, Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:4512-7. 2005
    ..These data suggest that control of HIV by vaccine-elicited HIV-specific T cell responses may be difficult, even when the T cell response has those characteristics predicted to provide optimal protection...
  49. pmc Virus inhibition activity of effector memory CD8(+) T cells determines simian immunodeficiency virus load in vaccinated monkeys after vaccine breakthrough infection
    Takuya Yamamoto
    Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
    J Virol 86:5877-84. 2012
    ..The ability to elicit such virus-specific EM CD8(+) T cells might contribute substantially to an efficacious HIV/AIDS vaccine, even after breakthrough infection...
  50. pmc Transient and persistent effects of IL-15 on lymphocyte homeostasis in nonhuman primates
    Enrico Lugli
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health NIH, Bethesda, MD, USA
    Blood 116:3238-48. 2010
    ..Thus, IL-15 generates a dramatic expansion of short-lived memory CD8 T cells and NK cells in immunocompetent macaques and has long-term effects on the balance of CD4(+) and CD8(+) T cells...
  51. pmc Increased IL-15 production is associated with higher susceptibility of memory CD4 T cells to simian immunodeficiency virus during acute infection
    Matthew D Eberly
    Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    J Immunol 182:1439-48. 2009
    ..Our results demonstrate that IL-15 contributes to the increased susceptibility of memory CD4 T cells to SIV during the early phase of acute SIV infection...
  52. pmc High avidity myeloid leukemia-associated antigen-specific CD8+ T cells preferentially reside in the bone marrow
    J Joseph Melenhorst
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 113:2238-44. 2009
    ..These data suggest that concomitant examination of bone marrow specimens in patients with myeloid leukemias might yield more definitive information in the search for immunologic prognosticators of clinical outcome...
  53. ncbi request reprint Differential susceptibility of leukocyte subsets to cytotoxic T cell killing: implications for HIV immunopathogenesis
    Jie Liu
    Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20895, USA
    Cytometry A 71:94-104. 2007
    ....
  54. ncbi request reprint T-cell dynamics during acute SIV infection
    Joseph J Mattapallil
    Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20895, USA
    AIDS 18:13-23. 2004
    ..To delineate T-cell dynamics during acute SIV infection, particularly of phenotypically defined memory T cell subsets...
  55. pmc Surface expression patterns of negative regulatory molecules identify determinants of virus-specific CD8+ T-cell exhaustion in HIV infection
    Takuya Yamamoto
    National Institutes of Health, Bethesda, MD, USA
    Blood 117:4805-15. 2011
    ..Thus, multiple coinhibitory receptors can affect the development of HIV-specific CD8(+) T-cell responses and, by extension, represent potential targets for new immune-based interventions in HIV-infected persons...
  56. pmc Selective expansion of polyfunctional pathogen-specific CD4(+) T cells in HIV-1-infected patients with immune reconstitution inflammatory syndrome
    Yolanda D Mahnke
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Blood 119:3105-12. 2012
    ..These studies were registered at www.clinical-trials.gov as NCT00557570 and NCT00286767...
  57. ncbi request reprint Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry
    Stephen P Perfetto
    Immunology Laboratory, Vaccine Research Center, NIAID, NIH, 40 Convent Dr, Room 5509, Bethesda, MD 20892, USA
    J Immunol Methods 313:199-208. 2006
    ..Amine reactive viability dyes are a powerful tool for fluorescence immunophenotyping experiments...
  58. pmc IL-15 expands unconventional CD8alphaalphaNK1.1+ T cells but not Valpha14Jalpha18+ NKT cells
    Masaki Terabe
    Vaccine Branch, National Cancer Institute, National Institute of Health, Bethesda, MD 20893, USA
    J Immunol 180:7276-86. 2008
    ..1(+) T cell subset, CD8alphaalphaNK1.1(+) T cells, and that IL-15-transgenic mice may be a useful resource for studying the functional relevance of CD8(+)NK1.1(+) T cells...
  59. pmc Immune protection of nonhuman primates against Ebola virus with single low-dose adenovirus vectors encoding modified GPs
    Nancy J Sullivan
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    PLoS Med 3:e177. 2006
    ..GP can exert cytopathic effects on transfected cells in vitro, and multiple GP forms have been identified in nature, raising the question of which would be optimal for a human vaccine...
  60. ncbi request reprint Massive infection and loss of memory CD4+ T cells in multiple tissues during acute SIV infection
    Joseph J Mattapallil
    Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA
    Nature 434:1093-7. 2005
    ..Our findings point to the importance of reducing the cell-associated viral load during acute infection through therapeutic or vaccination strategies...
  61. pmc Characterization of subsets of CD4+ memory T cells reveals early branched pathways of T cell differentiation in humans
    Kaimei Song
    Inflammation Biology Section, Laboratory of Molecular Immunology and Sections of Human Immunology and ImmunoTechnology, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:7916-21. 2005
    ..Rather, developmental pathways branch early on to yield effector/memory populations that are highly heterogeneous and multifunctional and have the potential to become stable resting cells...
  62. doi request reprint Early immunologic and virologic predictors of clinical HIV-1 disease progression
    Yolanda D Mahnke
    Immunotechnology Section, NIAID, NIH, Bethesda, MD, USA
    AIDS 27:697-706. 2013
    ....
  63. pmc DNA vaccine delivered by a needle-free injection device improves potency of priming for antibody and CD8+ T-cell responses after rAd5 boost in a randomized clinical trial
    Barney S Graham
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    PLoS ONE 8:e59340. 2013
    ..DNA vaccine immunogenicity has been limited by inefficient delivery. Needle-free delivery of DNA using a CO2-powered Biojector® device was compared to delivery by needle and syringe and evaluated for safety and immunogenicity...
  64. pmc Comparative analysis of the magnitude, quality, phenotype, and protective capacity of simian immunodeficiency virus gag-specific CD8+ T cells following human-, simian-, and chimpanzee-derived recombinant adenoviral vector immunization
    Kylie M Quinn
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 190:2720-35. 2013
    ..Collectively, these data provide the immunologic basis for using specific rAd vectors alone or as part of prime-boost regimens to induce CD8(+) T cells for rapid effector function or robust long-term memory, respectively...
  65. pmc CD4 T follicular helper cell dynamics during SIV infection
    Constantinos Petrovas
    Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA
    J Clin Invest 122:3281-94. 2012
    ..Therefore, chronic SIV does not disturb the ability of TFH cells to help B cell maturation and production of SIV-specific immunoglobulins...
  66. doi request reprint CD8+ cellular immunity mediates rAd5 vaccine protection against Ebola virus infection of nonhuman primates
    Nancy J Sullivan
    Vaccine Research Center, US National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA
    Nat Med 17:1128-31. 2011
    ..Understanding the immunologic mechanism of Ebola virus protection will facilitate the development of vaccines for Ebola and related hemorrhagic fever viruses in humans...
  67. pmc PD-1 is a regulator of virus-specific CD8+ T cell survival in HIV infection
    Constantinos Petrovas
    Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 203:2281-92. 2006
    ....
  68. pmc Immunologic and virologic events in early HIV infection predict subsequent rate of progression
    Anuradha Ganesan
    National Naval Medical Center, Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, Maryland 20892, USA
    J Infect Dis 201:272-84. 2010
    ..Because central memory T (T(CM)) cells play a critical role in the pathogenesis of simian immunodeficiency virus disease, we hypothesized that quantifying these cells in early HIV infection could provide prognostic information...
  69. pmc IL-15 delays suppression and fails to promote immune reconstitution in virally suppressed chronically SIV-infected macaques
    Enrico Lugli
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Blood 118:2520-9. 2011
    ....
  70. pmc Highly multiplexed quantitation of gene expression on single cells
    Maria H Dominguez
    Immunotechnology Section, Vaccine Research Center, NIAID, NIH, United States
    J Immunol Methods 391:133-45. 2013
    ....
  71. pmc Optimization and qualification of an 8-color intracellular cytokine staining assay for quantifying T cell responses in rhesus macaques for pre-clinical vaccine studies
    Mitzi M Donaldson
    Nonhuman Primate Immunogenicity Core, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA
    J Immunol Methods 386:10-21. 2012
    ....
  72. pmc Gene-based vaccination with a mismatched envelope protects against simian immunodeficiency virus infection in nonhuman primates
    Lukas Flatz
    Vaccine Research Center, NIAID, National Institutes of Health, Bethesda, Maryland, USA
    J Virol 86:7760-70. 2012
    ..These data indicate that a vaccine expressing a mismatched Env gene alone can prevent SIV infection in NHPs and identifies an immune correlate that may guide immunogen selection and immune monitoring for clinical efficacy trials...
  73. pmc Early immunologic correlates of HIV protection can be identified from computational analysis of complex multivariate T-cell flow cytometry assays
    Nima Aghaeepour
    Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC V5Z 1L3, Canada
    Bioinformatics 28:1009-16. 2012
    ..Thus, the value of PFC as a discovery tool is largely wasted...
  74. ncbi request reprint CCR2 identifies a stable population of human effector memory CD4+ T cells equipped for rapid recall response
    Hongwei H Zhang
    Inflammation Biology Section, Laboratory of Molecular Immunology, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 185:6646-63. 2010
    ....
  75. pmc Genetic immunization in the lung induces potent local and systemic immune responses
    Kaimei Song
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 107:22213-8. 2010
    ..Thus, genetic immunization in the lung offers a powerful platform approach to generating protective immune responses against respiratory pathogens...
  76. ncbi request reprint Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation
    Michael R Betts
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
    J Immunol Methods 281:65-78. 2003
    ....
  77. ncbi request reprint Toll-like receptor ligands modulate dendritic cells to augment cytomegalovirus- and HIV-1-specific T cell responses
    Karin Lore
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 3022, USA
    J Immunol 171:4320-8. 2003
    ....
  78. ncbi request reprint Ontogeny of gamma delta T cells in humans
    Stephen C De Rosa
    Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 172:1637-45. 2004
    ..This represents the earliest immunological maturation of any lymphocyte compartment in humans and most likely indicates the importance of these cells in controlling pathology due to common environmental challenges...
  79. pmc Preferential infection and depletion of Mycobacterium tuberculosis-specific CD4 T cells after HIV-1 infection
    Christof Geldmacher
    Immunology Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 207:2869-81. 2010
    ....
  80. pmc Good cell, bad cell: flow cytometry reveals T-cell subsets important in HIV disease
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Cytometry A 77:614-22. 2010
    ..Finally, we examine how flow cytometry studies have taught researchers about the disease process, and the potential for flow cytometry technology to guide treatment decisions and evaluate vaccine candidates in the future...
  81. pmc A West Nile virus DNA vaccine induces neutralizing antibody in healthy adults during a phase 1 clinical trial
    Julie E Martin
    Vaccine Research Center, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
    J Infect Dis 196:1732-40. 2007
    ..West Nile virus (WNV) is a mosquito-borne flavivirus that can cause severe meningitis and encephalitis in infected individuals. We report the safety and immunogenicity of a WNV DNA vaccine in its first phase 1 human study...
  82. pmc Alpha and lambda interferon together mediate suppression of CD4 T cells induced by respiratory syncytial virus
    Bo Chi
    Center for Biologics Evaluation and Research, Bethesda, MD 20892, USA
    J Virol 80:5032-40. 2006
    ..Defining the mechanism of RSV-induced suppression may guide vaccine design and provide insight into previously uncharacterized human T-cell responses and activities of interferons...
  83. ncbi request reprint Expression of CD57 defines replicative senescence and antigen-induced apoptotic death of CD8+ T cells
    Jason M Brenchley
    Vaccine Research Center and the Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 101:2711-20. 2003
    ..Thus, our studies define a phenotype associated with replicative senescence in HIV-specific CD8(+) T cells, which may have broad implications to other conditions associated with chronic antigenic stimulation...
  84. ncbi request reprint Beyond six colors: a new era in flow cytometry
    Stephen C De Rosa
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Nat Med 9:112-7. 2003
  85. pmc Elevated frequencies of highly activated CD4+ T cells in HIV+ patients developing immune reconstitution inflammatory syndrome
    Lis R V Antonelli
    Immunobiology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Blood 116:3818-27. 2010
    ..These studies are registered online at http://clinicaltrials.gov as NCT00557570 and NCT00286767...
  86. ncbi request reprint Viable infectious cell sorting in a BSL-3 facility
    Stephen P Perfetto
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 263:419-24. 2004
    ..With this system in place, aerosol containment can be measured quickly and efficiently, therefore reducing the risk to the operator when sorting viable infectious cells...
  87. pmc Demonstration of cross-protective vaccine immunity against an emerging pathogenic Ebolavirus Species
    Lisa E Hensley
    Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA
    PLoS Pathog 6:e1000904. 2010
    ....
  88. ncbi request reprint Accelerated vaccination for Ebola virus haemorrhagic fever in non-human primates
    Nancy J Sullivan
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bldg 40, Room 4502, MSC 3005, 40 Convent Drive, Bethesda, Maryland 20892 3005, USA
    Nature 424:681-4. 2003
    ..This accelerated vaccine provides an intervention that may help to limit the epidemic spread of Ebola, and is applicable to other viruses...
  89. doi request reprint Immunophenotyping of T cell subpopulations in HIV disease
    Pratip K Chattopadhyay
    National Institutes of Health, Bethesda, Maryland, USA
    Curr Protoc Immunol . 2005
    ....
  90. ncbi request reprint A live-cell assay to detect antigen-specific CD4+ T cells with diverse cytokine profiles
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Room 5509, Bethesda, Maryland 20892, USA
    Nat Med 11:1113-7. 2005
    ..For vaccine- or pathogen-specific responses, we found substantial heterogeneity in expression of CD154 and cytokines, suggesting previously unrecognized diversity in abilities of responding cells to stimulate APCs through CD40...
  91. pmc Safety (toxicity), pharmacokinetics, immunogenicity, and impact on elements of the normal immune system of recombinant human IL-15 in rhesus macaques
    Thomas A Waldmann
    Center for Cancer Research, National Cancer Institute, Frederick, MD, USA
    Blood 117:4787-95. 2011
    ....
  92. ncbi request reprint Measuring containment of viable infectious cell sorting in high-velocity cell sorters
    Stephen P Perfetto
    Vaccine Research Center, NAID, National Institute of Health, Bethesda, Maryland 20892 3015, USA
    Cytometry A 52:122-30. 2003
    ..With the advent of high-speed sorters, aerosols are a considerable safety concern when sorting viable infectious materials. We describe a four-part safety procedure for validating the containment...
  93. doi request reprint Flow cytometry and the future of vaccine development
    Diane L Bolton
    Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA
    Expert Rev Vaccines 8:779-89. 2009
    ..Finally, flow cytometry can also be used to analyze the contribution of innate immunity to vaccine efficacy and disease pathogenesis...
  94. pmc Antiretroviral therapy prior to acute viral replication preserves CD4 T cells in the periphery but not in rectal mucosa during acute simian immunodeficiency virus infection
    Muhamuda Kader
    Department of Microbiology and Immunology, Uniformed Services University, Room B4068, Bethesda, MD 20814, USA
    J Virol 82:11467-71. 2008
    ..Although early ART was of limited value in protecting the CD4 T cells in the rectal mucosa, the significant preservation of peripheral CD4 T cells could contribute to maintaining immune competence, leading to a better long-term outcome...
  95. doi request reprint The use of quantum dot nanocrystals in multicolor flow cytometry
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA
    Wiley Interdiscip Rev Nanomed Nanobiotechnol 2:334-48. 2010
    ..This article discusses the value of QDs in multicolor flow cytometry, introduces strategies to successfully incorporate QDs into routine use, and highlights emerging applications of the technology...
  96. ncbi request reprint A potent anti-HIV immunotoxin blocks spreading infection by primary HIV type 1 isolates in multiple cell types
    Kira K Lueders
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    AIDS Res Hum Retroviruses 20:145-50. 2004
    ....
  97. ncbi request reprint Seventeen-colour flow cytometry: unravelling the immune system
    Stephen P Perfetto
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Room 5507, Bethesda, Maryland 20892 3015, United States
    Nat Rev Immunol 4:648-55. 2004
  98. ncbi request reprint Immunocompetent T-cells with a memory-like phenotype are the dominant cell type following antibody-mediated T-cell depletion
    Jonathan P Pearl
    Transplantation Branch, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, USA
    Am J Transplant 5:465-74. 2005
    ..These data demonstrate that therapeutic depletion begets a limited population of functional memory-like T cells that are easily suppressed with certain immunosuppressants, but cannot be considered uniquely pro-tolerant...
  99. pmc Polyfunctional T cell responses are a hallmark of HIV-2 infection
    Melody G Duvall
    MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK
    Eur J Immunol 38:350-63. 2008
    ..Polyfunctional HIV-specific T cell responses are a hallmark of non-progressive HIV-2 infection and may be related to good clinical outcome in this setting...
  100. doi request reprint T-cell quality in memory and protection: implications for vaccine design
    Robert A Seder
    Cellular Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Rev Immunol 8:247-58. 2008
    ....
  101. ncbi request reprint Nine-color flow cytometry for accurate measurement of T cell subsets and cytokine responses. Part I: Panel design by an empiric approach
    Bridget E McLaughlin
    Division of Infectious Diseases, Internal Medicine, University of California Davis, Davis, CA, USA
    Cytometry A 73:400-10. 2008
    ..Careful reagent titration and testing of multiple candidate panels are necessary to ensure quality results in multiparametric measurements...