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Genomes and Genes | Mario RoedererSummaryAffiliation: National Institutes of Health Country: USA Publications
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How many events is enough? Are you positive?Mario Roederer
Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA
Cytometry A 73:384-5. 2008- Live-cell assay to detect antigen-specific CD4+ T-cell responses by CD154 expressionPratip K Chattopadhyay
Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
Nat Protoc 1:1-6. 2006..Unlike other assays, this method allows simultaneous assessment of other cell phenotypes or functions, is compatible with downstream RNA-based assays and preserves cell viability. This protocol can be completed in 9 h... - Intracellular cytokine optimization and standard operating procedureLaurie Lamoreaux
Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
Nat Protoc 1:1507-16. 2006.... - Quality assurance for polychromatic flow cytometryStephen P Perfetto
Flow Cytometry Core Facility, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
Nat Protoc 1:1522-30. 2006..All three aspects of this program should be performed upon installation, or whenever changes occur along the flow cytometer's optical path. However, only a few of these procedures need to be carried out on a routine basis... 
Polychromatic plots: graphical display of multidimensional dataMario Roederer
Vaccine Research Center, NIH, Bethesda, Maryland 20892 3015, USA
Cytometry A 73:868-74. 2008..In many ways, the display performs somewhat like an unsupervised cluster algorithm, by highlighting events of similar distributions in multivariate space...- Standardization of cytokine flow cytometry assaysHolden T Maecker
BD Biosciences, San Jose, USA
BMC Immunol 6:13. 2005.... - Flow cytometric analysis of vaccine responses: how many colors are enough?Mario Roederer
Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA
Clin Immunol 110:199-205. 2004..In this manuscript, we discuss these technologies, with a focus on assisting in the design and implementation of immunogenicity trials for future vaccine efforts... 
Interpretation of cellular proliferation data: Avoid the panglossianMario Roederer
Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland
Cytometry A 79:95-101. 2011..Published 2011 Wiley-Liss, Inc...- SPICE: exploration and analysis of post-cytometric complex multivariate datasetsMario Roederer
Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA
Cytometry A 79:167-74. 2011..While originally developed to support the analysis of T cell functional profiles, these techniques are amenable to a broad range of datatypes... - Optimized determination of T cell epitope responsesMario Roederer
Vaccine Research Center, NIAID, NIH, 40 Convent Drive, Room 5509, Bethesda, MD 20892, USA
J Immunol Methods 274:221-8. 2003..In addition, our results guide the design and implementation of the experiments to deconvolute the responses to individual peptide epitopes... - Phase 1 safety and immunogenicity evaluation of a multiclade HIV-1 DNA candidate vaccineBarney S Graham
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 3017, USA
J Infect Dis 194:1650-60. 2006..Vaccine Research Center (VRC) 004 is the first phase 1 dose-escalation study of a multiclade HIV-1 DNA vaccine... - Acquisition of direct antiviral effector functions by CMV-specific CD4+ T lymphocytes with cellular maturationJoseph P Casazza
Immunology Laboratory, Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID, National Institutes of Health (NIH, Bethesda, MD 20892, USA
J Exp Med 203:2865-77. 2006..Thus, mature CMV-specific CD4+ T cells exhibit distinct functional properties reminiscent of antiviral CD8+ T lymphocytes... - The functional profile of primary human antiviral CD8+ T cell effector activity is dictated by cognate peptide concentrationMichael R Betts
Immunology Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 172:6407-17. 2004..The inherent ability of viruses to induce high or low Ag states may be the primary determinant of the cytokine vs cytolytic nature of the virus-specific CD8(+) T cell response... - Priming immunization with DNA augments immunogenicity of recombinant adenoviral vectors for both HIV-1 specific antibody and T-cell responsesRichard A Koup
Vaccine Research Center, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
PLoS ONE 5:e9015. 2010..Prime-boost regimens using heterologous gene-based vaccine vectors have induced potent, polyfunctional T cell responses in preclinical studies... - Phase I clinical evaluation of a six-plasmid multiclade HIV-1 DNA candidate vaccineAndrew T Catanzaro
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Building 40, Bethesda, MD 20892 3017, USA
Vaccine 25:4085-92. 2007..Compared to a four-plasmid product, Gag- and Nef-specific T cell responses were improved, while Env-specific responses were maintained. This candidate vaccine has now advanced to Phase II evaluation... - Phase 1 safety and immunogenicity evaluation of a multiclade HIV-1 candidate vaccine delivered by a replication-defective recombinant adenovirus vectorAndrew T Catanzaro
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-3017, USA
J Infect Dis 194:1638-49. 2006..This multiclade rAd5 HIV-1 vaccine is now being evaluated in combination with a multiclade HIV-1 DNA plasmid vaccine... - Toll-like receptor agonists influence the magnitude and quality of memory T cell responses after prime-boost immunization in nonhuman primatesUlrike Wille-Reece
Cellular Immunology Section, Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA
J Exp Med 203:1249-58. 2006..These data provide insights for designing prime-boost immunization regimens to optimize Th1 and CD8+ T cell responses... - SIV-specific CD8+ T cells express high levels of PD1 and cytokines but have impaired proliferative capacity in acute and chronic SIVmac251 infectionConstantinos Petrovas
Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases NIH, 40 Convent Drive, Bethesda, MD 20892, USA
Blood 110:928-36. 2007..Manipulation of the interaction of PD-1 with its ligands could thus potentially restore the CD8+ T-cell responses in SIV infection... - Differential association of programmed death-1 and CD57 with ex vivo survival of CD8+ T cells in HIV infectionConstantinos Petrovas
Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20814, USA
J Immunol 183:1120-32. 2009..Thus, our data further support the role of PD-1 as a preapoptotic factor for CD8(+) T cells in HIV infection... - Public clonotype usage identifies protective Gag-specific CD8+ T cell responses in SIV infectionDavid A Price
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Exp Med 206:923-36. 2009..Thus, the pattern of antigen-specific clonotype recruitment within a protective CD8(+) T cell population is a prognostic indicator of vaccine efficacy and biological outcome in an AIDS virus infection... - High frequencies of resting CD4+ T cells containing integrated viral DNA are found in rhesus macaques during acute lentivirus infectionsYoshiaki Nishimura
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 106:8015-20. 2009..Prompt and sustained interventions are therefore required to block the rapid systemic dissemination of virus and prevent an otherwise fatal clinical outcome... - The transfer of adaptive immunity to CMV during hematopoietic stem cell transplantation is dependent on the specificity and phenotype of CMV-specific T cells in the donorPhillip Scheinberg
Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
Blood 114:5071-80. 2009.... - Reduced protection from simian immunodeficiency virus SIVmac251 infection afforded by memory CD8+ T cells induced by vaccination during CD4+ T-cell deficiencyMonica Vaccari
Animal Models and Retroviral Vaccines Section, National Cancer Institute, Bldg 41, Room D804, Bethesda, MD 20892, USA
J Virol 82:9629-38. 2008.... - Replication-defective adenovirus vectors with multiple deletions do not induce measurable vector-specific T cells in human trialsRichard A Koup
Immunology Laboratory, Immunology Core Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
J Virol 83:6318-22. 2009..These data indicate that rAd5-based vaccines containing deletions in the E1, E3, and E4 regions do not induce appreciable expansion of vector-specific CD4(+) T cells... - A SARS DNA vaccine induces neutralizing antibody and cellular immune responses in healthy adults in a Phase I clinical trialJulie E Martin
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, MSC 2610, Bethesda, MD 20892 3017, USA
Vaccine 26:6338-43. 2008..Over 8000 cases and 900 deaths occurred during the epidemic. We report the safety and immunogenicity of a SARS DNA vaccine in a Phase I human study... - Rational design of envelope identifies broadly neutralizing human monoclonal antibodies to HIV-1Xueling Wu
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Science 329:856-61. 2010..Exceptionally broad HIV-1 neutralization can be achieved with individual antibodies targeted to the functionally conserved CD4bs of glycoprotein 120, an important insight for future HIV-1 vaccine design... - T-cell subsets that harbor human immunodeficiency virus (HIV) in vivo: implications for HIV pathogenesisJason M Brenchley
Human Immunology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
J Virol 78:1160-8. 2004..These data illuminate the underlying mechanisms that distort T-cell homeostasis in HIV infection... - Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramersPratip K Chattopadhyay
Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Cytometry A 73:1001-9. 2008.... - The cytolytic enzymes granyzme A, granzyme B, and perforin: expression patterns, cell distribution, and their relationship to cell maturity and bright CD57 expressionPratip K Chattopadhyay
Immunotechnology Section, Laboratory of Immunology, Vaccine Research Center, National Institutes of Health, Bethesda, MD, USA
J Leukoc Biol 85:88-97. 2009..Thus, the use of CD57 provides a means to easily isolate viable cells with high cytolytic potential, without the need for lethal fixation/permeabilization techniques... - IL-10 production differentially influences the magnitude, quality, and protective capacity of Th1 responses depending on the vaccine platformPatricia A Darrah
Cellular Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Exp Med 207:1421-33. 2010..Overall, we delineate distinct mechanisms by which vaccines elicit protective Th1 responses and underscore the importance of multifunctional CD4(+) T cells... - Systemic vaccination prevents the total destruction of mucosal CD4 T cells during acute SIV challengeJoseph J Mattapallil
Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA
J Med Primatol 35:217-24. 2006..The primary objective of this study was to determine if systemic vaccination with DNA/rAd-5 encoding SIV-mac239-env, gag and pol could prevent the destruction of CD4 T cells in mucosal tissues... - Avidity for antigen shapes clonal dominance in CD8+ T cell populations specific for persistent DNA virusesDavid A Price
Human Immunology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Exp Med 202:1349-61. 2005..Vaccine strategies that reconstruct these biological processes could generate T cell populations that mediate optimal delivery of antiviral effector function... - Trafficking, persistence, and activation state of adoptively transferred allogeneic and autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones during acute and chronic infection of rhesus macaquesDiane L Bolton
Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 184:303-14. 2010..The lack of impact following transfer of such a large number of functional Ag-specific CD8(+) T cells on SIV replication may reflect the magnitude of the immune response required to contain the virus... - Vaccination in humans generates broad T cell cytokine responsesStephen C De Rosa
Vaccine Research Center, National Institute of Allery and Infectious Diseases, NIH, Bethesda, MD 20892, USA
J Immunol 173:5372-80. 2004..The presence and variability of these complex response profiles introduce the possibility that selective functional expression patterns may provide correlates for vaccine efficacy or disease progression... - Immunization with vaccinia virus induces polyfunctional and phenotypically distinctive CD8(+) T cell responsesMelissa L Precopio
Immunology Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Exp Med 204:1405-16. 2007..This quality of the CD8(+) T cell response may be at least partially responsible for the profound efficacy of these vaccines in protection against smallpox and serves as a benchmark against which other vaccines can be evaluated... - Amine-reactive dyes for dead cell discrimination in fixed samplesStephen P Perfetto
Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
Curr Protoc Cytom . 2010..This unit describes procedures, troubleshooting, and outcomes for using the two most commonly used amine-reactive dyes, ViViD and Aqua Blue... - Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometryPratip K Chattopadhyay
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
Nat Med 12:972-7. 2006.... - HIV Gag protein conjugated to a Toll-like receptor 7/8 agonist improves the magnitude and quality of Th1 and CD8+ T cell responses in nonhuman primatesUlrike Wille-Reece
Cellular Immunology Section, Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 102:15190-4. 2005..This type of vaccine formulation should have utility in preventive or therapeutic vaccines in which humoral and cellular immunity is required... - Autocrine production of beta-chemokines protects CMV-Specific CD4 T cells from HIV infectionJoseph P Casazza
Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
PLoS Pathog 5:e1000646. 2009..These data suggest that CD4+ T cells which produce MIP-1alpha and MIP-1beta bind these chemokines in an autocrine fashion which decreases the risk of in vivo HIV infection... - HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T cellsMichael R Betts
Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Blood 107:4781-9. 2006..Thus, rather than quantity or phenotype, the quality of the CD8(+) T-cell functional response serves as an immune correlate of HIV disease progression and a potential qualifying factor for evaluation of HIV vaccine efficacy... - Safety and immunogenicity of a Gag-Pol candidate HIV-1 DNA vaccine administered by a needle-free device in HIV-1-seronegative subjectsJorge A Tavel
Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Acquir Immune Defic Syndr 44:601-5. 2007..To evaluate the safety and immunogenicity of a candidate HIV DNA vaccine administered using a needle-free device... - Characterization of functional and phenotypic changes in anti-Gag vaccine-induced T cell responses and their role in protection after HIV-1 infectionMichael R Betts
Laboratory of Immunology, Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 102:4512-7. 2005..These data suggest that control of HIV by vaccine-elicited HIV-specific T cell responses may be difficult, even when the T cell response has those characteristics predicted to provide optimal protection... - Vaccination preserves CD4 memory T cells during acute simian immunodeficiency virus challengeJoseph J Mattapallil
Vaccine Research Center, National Institute of Allergy and Infectious Disease (NIAID, National Institutes of Health (NIH, Bethesda, MD 20892, USA
J Exp Med 203:1533-41. 2006.... - Virus inhibition activity of effector memory CD8(+) T cells determines simian immunodeficiency virus load in vaccinated monkeys after vaccine breakthrough infectionTakuya Yamamoto
Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
J Virol 86:5877-84. 2012..The ability to elicit such virus-specific EM CD8(+) T cells might contribute substantially to an efficacious HIV/AIDS vaccine, even after breakthrough infection... - Transient and persistent effects of IL-15 on lymphocyte homeostasis in nonhuman primatesEnrico Lugli
Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health NIH, Bethesda, MD, USA
Blood 116:3238-48. 2010..Thus, IL-15 generates a dramatic expansion of short-lived memory CD8 T cells and NK cells in immunocompetent macaques and has long-term effects on the balance of CD4(+) and CD8(+) T cells... - Increased IL-15 production is associated with higher susceptibility of memory CD4 T cells to simian immunodeficiency virus during acute infectionMatthew D Eberly
Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
J Immunol 182:1439-48. 2009..Our results demonstrate that IL-15 contributes to the increased susceptibility of memory CD4 T cells to SIV during the early phase of acute SIV infection... - Surface expression patterns of negative regulatory molecules identify determinants of virus-specific CD8+ T-cell exhaustion in HIV infectionTakuya Yamamoto
National Institutes of Health, Bethesda, MD, USA
Blood 117:4805-15. 2011..Thus, multiple coinhibitory receptors can affect the development of HIV-specific CD8(+) T-cell responses and, by extension, represent potential targets for new immune-based interventions in HIV-infected persons... - Differential susceptibility of leukocyte subsets to cytotoxic T cell killing: implications for HIV immunopathogenesisJie Liu
Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20895, USA
Cytometry A 71:94-104. 2007.... - Characterization of subsets of CD4+ memory T cells reveals early branched pathways of T cell differentiation in humansKaimei Song
Inflammation Biology Section, Laboratory of Molecular Immunology and Sections of Human Immunology and ImmunoTechnology, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 102:7916-21. 2005..Rather, developmental pathways branch early on to yield effector/memory populations that are highly heterogeneous and multifunctional and have the potential to become stable resting cells... - High avidity myeloid leukemia-associated antigen-specific CD8+ T cells preferentially reside in the bone marrowJ Joseph Melenhorst
Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Blood 113:2238-44. 2009..These data suggest that concomitant examination of bone marrow specimens in patients with myeloid leukemias might yield more definitive information in the search for immunologic prognosticators of clinical outcome... - Selective expansion of polyfunctional pathogen-specific CD4(+) T cells in HIV-1-infected patients with immune reconstitution inflammatory syndromeYolanda D Mahnke
Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Blood 119:3105-12. 2012..These studies were registered at www.clinical-trials.gov as NCT00557570 and NCT00286767... - IL-15 expands unconventional CD8alphaalphaNK1.1+ T cells but not Valpha14Jalpha18+ NKT cellsMasaki Terabe
Vaccine Branch, National Cancer Institute, National Institute of Health, Bethesda, MD 20893, USA
J Immunol 180:7276-86. 2008..1(+) T cell subset, CD8alphaalphaNK1.1(+) T cells, and that IL-15-transgenic mice may be a useful resource for studying the functional relevance of CD8(+)NK1.1(+) T cells... - Differential specificity and immunogenicity of adenovirus type 5 neutralizing antibodies elicited by natural infection or immunizationCheng Cheng
Vaccine Research Center, NIAID, National Institutes of Health, Bethesda, MD 20892 3005, USA
J Virol 84:630-8. 2010..These results have implications for future AIDS vaccine trials and the design of next-generation gene-based vaccine vectors... - Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometryStephen P Perfetto
Immunology Laboratory, Vaccine Research Center, NIAID, NIH, 40 Convent Dr, Room 5509, Bethesda, MD 20892, USA
J Immunol Methods 313:199-208. 2006..Amine reactive viability dyes are a powerful tool for fluorescence immunophenotyping experiments... - Immune protection of nonhuman primates against Ebola virus with single low-dose adenovirus vectors encoding modified GPsNancy J Sullivan
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
PLoS Med 3:e177. 2006..GP can exert cytopathic effects on transfected cells in vitro, and multiple GP forms have been identified in nature, raising the question of which would be optimal for a human vaccine... - CD4 T follicular helper cell dynamics during SIV infectionConstantinos Petrovas
Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA
J Clin Invest 122:3281-94. 2012..Therefore, chronic SIV does not disturb the ability of TFH cells to help B cell maturation and production of SIV-specific immunoglobulins... - IL-15 delays suppression and fails to promote immune reconstitution in virally suppressed chronically SIV-infected macaquesEnrico Lugli
Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Blood 118:2520-9. 2011.... - CD8+ cellular immunity mediates rAd5 vaccine protection against Ebola virus infection of nonhuman primatesNancy J Sullivan
Vaccine Research Center, US National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA
Nat Med 17:1128-31. 2011..Understanding the immunologic mechanism of Ebola virus protection will facilitate the development of vaccines for Ebola and related hemorrhagic fever viruses in humans... - PD-1 is a regulator of virus-specific CD8+ T cell survival in HIV infectionConstantinos Petrovas
Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Exp Med 203:2281-92. 2006.... - Immunologic and virologic events in early HIV infection predict subsequent rate of progressionAnuradha Ganesan
National Naval Medical Center, Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, Maryland 20892, USA
J Infect Dis 201:272-84. 2010..Because central memory T (T(CM)) cells play a critical role in the pathogenesis of simian immunodeficiency virus disease, we hypothesized that quantifying these cells in early HIV infection could provide prognostic information... - Optimization and qualification of an 8-color intracellular cytokine staining assay for quantifying T cell responses in rhesus macaques for pre-clinical vaccine studiesMitzi M Donaldson
Nonhuman Primate Immunogenicity Core, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA
J Immunol Methods 386:10-21. 2012.... - Gene-based vaccination with a mismatched envelope protects against simian immunodeficiency virus infection in nonhuman primatesLukas Flatz
Vaccine Research Center, NIAID, National Institutes of Health, Bethesda, Maryland, USA
J Virol 86:7760-70. 2012..These data indicate that a vaccine expressing a mismatched Env gene alone can prevent SIV infection in NHPs and identifies an immune correlate that may guide immunogen selection and immune monitoring for clinical efficacy trials... - Early immunologic correlates of HIV protection can be identified from computational analysis of complex multivariate T-cell flow cytometry assaysNima Aghaeepour
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC V5Z 1L3, Canada
Bioinformatics 28:1009-16. 2012..Thus, the value of PFC as a discovery tool is largely wasted... - CCR2 identifies a stable population of human effector memory CD4+ T cells equipped for rapid recall responseHongwei H Zhang
Inflammation Biology Section, Laboratory of Molecular Immunology, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 185:6646-63. 2010.... - Preferential infection and depletion of Mycobacterium tuberculosis-specific CD4 T cells after HIV-1 infectionChristof Geldmacher
Immunology Laboratory, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
J Exp Med 207:2869-81. 2010.... - Genetic immunization in the lung induces potent local and systemic immune responsesKaimei Song
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 107:22213-8. 2010..Thus, genetic immunization in the lung offers a powerful platform approach to generating protective immune responses against respiratory pathogens... - Ontogeny of gamma delta T cells in humansStephen C De Rosa
Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 172:1637-45. 2004..This represents the earliest immunological maturation of any lymphocyte compartment in humans and most likely indicates the importance of these cells in controlling pathology due to common environmental challenges... - Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulationMichael R Betts
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
J Immunol Methods 281:65-78. 2003.... - Toll-like receptor ligands modulate dendritic cells to augment cytomegalovirus- and HIV-1-specific T cell responsesKarin Lore
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 3022, USA
J Immunol 171:4320-8. 2003.... - Massive infection and loss of memory CD4+ T cells in multiple tissues during acute SIV infectionJoseph J Mattapallil
Immunotechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA
Nature 434:1093-7. 2005..Our findings point to the importance of reducing the cell-associated viral load during acute infection through therapeutic or vaccination strategies... - T-cell dynamics during acute SIV infectionJoseph J Mattapallil
Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20895, USA
AIDS 18:13-23. 2004..Changes in homeostasis and associated immunopathogenesis can no longer be accurately described simply by measuring naive and memory T-cell subsets... - Good cell, bad cell: flow cytometry reveals T-cell subsets important in HIV diseasePratip K Chattopadhyay
Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Cytometry A 77:614-22. 2010..Finally, we examine how flow cytometry studies have taught researchers about the disease process, and the potential for flow cytometry technology to guide treatment decisions and evaluate vaccine candidates in the future... - A West Nile virus DNA vaccine induces neutralizing antibody in healthy adults during a phase 1 clinical trialJulie E Martin
Vaccine Research Center, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, USA
J Infect Dis 196:1732-40. 2007..West Nile virus (WNV) is a mosquito-borne flavivirus that can cause severe meningitis and encephalitis in infected individuals. We report the safety and immunogenicity of a WNV DNA vaccine in its first phase 1 human study... - Alpha and lambda interferon together mediate suppression of CD4 T cells induced by respiratory syncytial virusBo Chi
Center for Biologics Evaluation and Research, Bethesda, MD 20892, USA
J Virol 80:5032-40. 2006..Defining the mechanism of RSV-induced suppression may guide vaccine design and provide insight into previously uncharacterized human T-cell responses and activities of interferons... - Beyond six colors: a new era in flow cytometryStephen C De Rosa
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
Nat Med 9:112-7. 2003 - Elevated frequencies of highly activated CD4+ T cells in HIV+ patients developing immune reconstitution inflammatory syndromeLis R V Antonelli
Immunobiology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Blood 116:3818-27. 2010..These studies are registered online at http://clinicaltrials.gov as NCT00557570 and NCT00286767... - Expression of CD57 defines replicative senescence and antigen-induced apoptotic death of CD8+ T cellsJason M Brenchley
Vaccine Research Center and the Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Blood 101:2711-20. 2003..Thus, our studies define a phenotype associated with replicative senescence in HIV-specific CD8(+) T cells, which may have broad implications to other conditions associated with chronic antigenic stimulation... - Viable infectious cell sorting in a BSL-3 facilityStephen P Perfetto
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Methods Mol Biol 263:419-24. 2004..With this system in place, aerosol containment can be measured quickly and efficiently, therefore reducing the risk to the operator when sorting viable infectious cells... - Immunophenotyping of T cell subpopulations in HIV diseasePratip K Chattopadhyay
National Institutes of Health, Bethesda, Maryland, USA
Curr Protoc Immunol . 2005.... - Accelerated vaccination for Ebola virus haemorrhagic fever in non-human primatesNancy J Sullivan
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bldg. 40, Room 4502, MSC 3005, 40 Convent Drive, Bethesda, Maryland 20892-3005, USA
Nature 424:681-4. 2003..This accelerated vaccine provides an intervention that may help to limit the epidemic spread of Ebola, and is applicable to other viruses... - A live-cell assay to detect antigen-specific CD4+ T cells with diverse cytokine profilesPratip K Chattopadhyay
Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Room 5509, Bethesda, Maryland 20892, USA
Nat Med 11:1113-7. 2005..For vaccine- or pathogen-specific responses, we found substantial heterogeneity in expression of CD154 and cytokines, suggesting previously unrecognized diversity in abilities of responding cells to stimulate APCs through CD40... - Demonstration of cross-protective vaccine immunity against an emerging pathogenic Ebolavirus SpeciesLisa E Hensley
Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA
PLoS Pathog 6:e1000904. 2010.... - Safety (toxicity), pharmacokinetics, immunogenicity, and impact on elements of the normal immune system of recombinant human IL-15 in rhesus macaquesThomas A Waldmann
Center for Cancer Research, National Cancer Institute, Frederick, MD, USA
Blood 117:4787-95. 2011.... - Antiretroviral therapy prior to acute viral replication preserves CD4 T cells in the periphery but not in rectal mucosa during acute simian immunodeficiency virus infectionMuhamuda Kader
Department of Microbiology and Immunology, Uniformed Services University, Room B4068, Bethesda, MD 20814, USA
J Virol 82:11467-71. 2008..Although early ART was of limited value in protecting the CD4 T cells in the rectal mucosa, the significant preservation of peripheral CD4 T cells could contribute to maintaining immune competence, leading to a better long-term outcome... - A potent anti-HIV immunotoxin blocks spreading infection by primary HIV type 1 isolates in multiple cell typesKira K Lueders
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
AIDS Res Hum Retroviruses 20:145-50. 2004.... - Seventeen-colour flow cytometry: unravelling the immune systemStephen P Perfetto
Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Room 5507, Bethesda, Maryland 20892-3015, United States
Nat Rev Immunol 4:648-55. 2004 - Measuring containment of viable infectious cell sorting in high-velocity cell sortersStephen P Perfetto
Vaccine Research Center, NAID, National Institute of Health, Bethesda, Maryland 20892 3015, USA
Cytometry A 52:122-30. 2003..With the advent of high-speed sorters, aerosols are a considerable safety concern when sorting viable infectious materials. We describe a four-part safety procedure for validating the containment... - The use of quantum dot nanocrystals in multicolor flow cytometryPratip K Chattopadhyay
Immunotechnology Section, Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA
Wiley Interdiscip Rev Nanomed Nanobiotechnol 2:334-48. 2010..This article discusses the value of QDs in multicolor flow cytometry, introduces strategies to successfully incorporate QDs into routine use, and highlights emerging applications of the technology... - Flow cytometry and the future of vaccine developmentDiane L Bolton
Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA
Expert Rev Vaccines 8:779-89. 2009..Finally, flow cytometry can also be used to analyze the contribution of innate immunity to vaccine efficacy and disease pathogenesis... - Magnitude and quality of vaccine-elicited T-cell responses in the control of immunodeficiency virus replication in rhesus monkeysYue Sun
Department of Medicine, Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
J Virol 82:8812-9. 2008..These observations highlight the contributions of both the quality and the magnitude of vaccine-elicited cellular immune responses in the control of immunodeficiency virus replication... - Increased immunofluorescence sensitivity using 532 nm laser excitationStephen P Perfetto
Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892 3015, USA
Cytometry A 71:73-9. 2007..We evaluated the use of a high power, diode pulsed solid-state laser emitting 532 nm light for immunofluorescence applications. We compared the sensitivity and utility of this laser with the standard 488 nm excitation... - Nine-color flow cytometry for accurate measurement of T cell subsets and cytokine responses. Part I: Panel design by an empiric approachBridget E McLaughlin
Division of Infectious Diseases, Internal Medicine, University of California Davis, Davis, CA, USA
Cytometry A 73:400-10. 2008..Careful reagent titration and testing of multiple candidate panels are necessary to ensure quality results in multiparametric measurements... - Measurement of antigen specific immune responses: 2006 updateGiuseppina Li Pira
Viral Immunology, Advanced Biotechnology Center, Largo Benzi 10, 16132 Genoa, Italy
Cytometry B Clin Cytom 72:77-85. 2007.... - Nine-color flow cytometry for accurate measurement of T cell subsets and cytokine responses. Part II: Panel performance across different instrument platformsBridget E McLaughlin
University of California Davis, Internal Medicine, Infectious Diseases, Davis, CA, USA
Cytometry A 73:411-20. 2008.... - A DNA vaccine for Ebola virus is safe and immunogenic in a phase I clinical trialJulie E Martin
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes, Bethesda, MD 20892 3017, USA
Clin Vaccine Immunol 13:1267-77. 2006.... - T-cell quality in memory and protection: implications for vaccine designRobert A Seder
Cellular Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
Nat Rev Immunol 8:247-58. 2008.... - Estimating the infectivity of CCR5-tropic simian immunodeficiency virus SIV(mac251) in the gutDavid P Wilson
Department of Haematology, Prince of Wales Hospital, University of New South Wales, Kensington, New South Wales, Australia
J Virol 81:8025-9. 2007..6P infection, but this higher infectivity is offset by a lower average peak viral load in SIV(mac251). Thus, the dynamics of target cell infection and death are remarkably similar between a CXCR4- and a CCR5-tropic infection in vivo... - Infectious molecular clones from a simian immunodeficiency virus-infected rapid-progressor (RP) macaque: evidence of differential selection of RP-specific envelope mutations in vitro and in vivoTakeo Kuwata
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases/NIH, Building 4, Rm. B1-33, 4 Center Drive, Bethesda, MD 20892, USA
J Virol 80:1463-75. 2006..Therefore, tissue culture may not provide a good surrogate for replication of RP variants in macaques. These infectious clones will provide a valuable reagent to study the roles of specific viral variants in rapid progression in vivo... - Compensation in flow cytometryMario Roederer
Vaccine Research Center NALID, NIH, Bethesda, Maryland, USA
Curr Protoc Cytom . 2002..Many example figures are supplied to give the reader a clear indication of how the author believes compensation should be handled... - Polyfunctional T cell responses are a hallmark of HIV-2 infectionMelody G Duvall
MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK
Eur J Immunol 38:350-63. 2008..Polyfunctional HIV-specific T cell responses are a hallmark of non-progressive HIV-2 infection and may be related to good clinical outcome in this setting... - Human immunodeficiency virus type 1 neutralization measured by flow cytometric quantitation of single-round infection of primary human T cellsJohn R Mascola
Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
J Virol 76:4810-21. 2002..The precision and reproducibility of this assay will facilitate the measurement of HIV-1 neutralization, particularly incrementally improved neutralizing antibody responses generated by new candidate vaccines...