S G Rhee

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Reduction of cysteine sulfinic acid by sulfiredoxin is specific to 2-cys peroxiredoxins
    Hyun Ae Woo
    Laboratory of Cell Signaling and Laboratory of Biophysical Chemistry, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:3125-8. 2005
  2. pmc Dynein light chain LC8 negatively regulates NF-kappaB through the redox-dependent interaction with IkappaBalpha
    Yuyeon Jung
    Department of Life Science, Division of Life and Pharmaceutical Sciences, and Center for Cell Signaling and Drug Discovery Research, Ewha Womans University, 11 1 Daehyun Dong, Seodaemun gu, Seoul, Korea
    J Biol Chem 283:23863-71. 2008
  3. ncbi request reprint Redox signaling: hydrogen peroxide as intracellular messenger
    S G Rhee
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Exp Mol Med 31:53-9. 1999
  4. ncbi request reprint Regulation of phosphoinositide-specific phospholipase C
    S G Rhee
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 0320, USA
    Annu Rev Biochem 70:281-312. 2001
  5. ncbi request reprint Intracellular messenger function of hydrogen peroxide and its regulation by peroxiredoxins
    Sue Goo Rhee
    Laboratory of Cell Signaling, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Curr Opin Cell Biol 17:183-9. 2005
  6. ncbi request reprint Cellular regulation by hydrogen peroxide
    Sue Goo Rhee
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    J Am Soc Nephrol 14:S211-5. 2003
  7. ncbi request reprint Reversible inactivation of the tumor suppressor PTEN by H2O2
    Seung Rock Lee
    Laboratory of Cell Signaling, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 8015, USA
    J Biol Chem 277:20336-42. 2002
  8. ncbi request reprint Controlled elimination of intracellular H(2)O(2): regulation of peroxiredoxin, catalase, and glutathione peroxidase via post-translational modification
    Sue Goo Rhee
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Antioxid Redox Signal 7:619-26. 2005
  9. ncbi request reprint Peroxiredoxins: a historical overview and speculative preview of novel mechanisms and emerging concepts in cell signaling
    Sue Goo Rhee
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 38:1543-52. 2005
  10. ncbi request reprint Cloning, sequencing, purification, and Gq-dependent activation of phospholipase C-beta 3
    D Y Jhon
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 268:6654-61. 1993

Collaborators

Detail Information

Publications71

  1. ncbi request reprint Reduction of cysteine sulfinic acid by sulfiredoxin is specific to 2-cys peroxiredoxins
    Hyun Ae Woo
    Laboratory of Cell Signaling and Laboratory of Biophysical Chemistry, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:3125-8. 2005
    ..These results suggest that reduction of Cys-SO2H by Srx is specific to 2-Cys Prx isoforms. For proteins such as Prx VI and GAPDH, sulfinic acid formation might be an irreversible process that causes protein damage...
  2. pmc Dynein light chain LC8 negatively regulates NF-kappaB through the redox-dependent interaction with IkappaBalpha
    Yuyeon Jung
    Department of Life Science, Division of Life and Pharmaceutical Sciences, and Center for Cell Signaling and Drug Discovery Research, Ewha Womans University, 11 1 Daehyun Dong, Seodaemun gu, Seoul, Korea
    J Biol Chem 283:23863-71. 2008
    ..Together, our results indicate that LC8 binds IkappaBalpha in a redox-dependent manner and thereby prevents its phosphorylation by IKK. TRP14 contributes to this inhibitory activity by maintaining LC8 in a reduced state...
  3. ncbi request reprint Redox signaling: hydrogen peroxide as intracellular messenger
    S G Rhee
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Exp Mol Med 31:53-9. 1999
    ..The goal of this report is to review our current knowledge of ROS-regulated signal transduction and propose future directions...
  4. ncbi request reprint Regulation of phosphoinositide-specific phospholipase C
    S G Rhee
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 0320, USA
    Annu Rev Biochem 70:281-312. 2001
    ....
  5. ncbi request reprint Intracellular messenger function of hydrogen peroxide and its regulation by peroxiredoxins
    Sue Goo Rhee
    Laboratory of Cell Signaling, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Curr Opin Cell Biol 17:183-9. 2005
    ....
  6. ncbi request reprint Cellular regulation by hydrogen peroxide
    Sue Goo Rhee
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    J Am Soc Nephrol 14:S211-5. 2003
    ..Furthermore, the activity of peroxiredoxin enzymes seems to be regulated via protein phosphorylation as in the case of many other intracellular messenger metabolizing enzymes...
  7. ncbi request reprint Reversible inactivation of the tumor suppressor PTEN by H2O2
    Seung Rock Lee
    Laboratory of Cell Signaling, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 8015, USA
    J Biol Chem 277:20336-42. 2002
    ....
  8. ncbi request reprint Controlled elimination of intracellular H(2)O(2): regulation of peroxiredoxin, catalase, and glutathione peroxidase via post-translational modification
    Sue Goo Rhee
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Antioxid Redox Signal 7:619-26. 2005
    ..Similarily, GPx1 is activated by c-Abl- and Arg-mediated phosphorylation. The tyrosine phosphorylation is critical for ubiquitination-dependent degradation of catalase...
  9. ncbi request reprint Peroxiredoxins: a historical overview and speculative preview of novel mechanisms and emerging concepts in cell signaling
    Sue Goo Rhee
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 38:1543-52. 2005
    ..This reversible overoxidation may represent an adaptation unique to eukaryotic cells that accommodates the intracellular messenger function of H(2)O(2), but experimental validation of such speculation is yet to come...
  10. ncbi request reprint Cloning, sequencing, purification, and Gq-dependent activation of phospholipase C-beta 3
    D Y Jhon
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 268:6654-61. 1993
    ....
  11. ncbi request reprint Identification of a new type of mammalian peroxiredoxin that forms an intramolecular disulfide as a reaction intermediate
    M S Seo
    Laboratory of Cell Signaling and Department of Extramural Affairs, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 275:20346-54. 2000
    ....
  12. pmc Activation of rat brain phospholipase D by ADP-ribosylation factors 1,5, and 6: separation of ADP-ribosylation factor-dependent and oleate-dependent enzymes
    D Massenburg
    Laboratory of Cellular Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 91:11718-22. 1994
    ....
  13. ncbi request reprint Cloning and sequence of multiple forms of phospholipase C
    P G Suh
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892
    Cell 54:161-9. 1988
    ..Those of PLC-I and III were short and appeared to be unrelated to these tyrosine kinases. The physiological implications of the multiple species of PLC enzymes are discussed...
  14. ncbi request reprint Characterization of a mammalian peroxiredoxin that contains one conserved cysteine
    S W Kang
    Laboratory of Cell Signaling, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 273:6303-11. 1998
    ..Thus, the primary cellular function of 1-Cys Prx appears to be to reduce peroxides with the use of electrons provided by an as yet unidentified source; the enzyme therefore represents a new type of peroxidase...
  15. ncbi request reprint Cloning, sequencing, and mutation of thiol-specific antioxidant gene of Saccharomyces cerevisiae
    H Z Chae
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 268:16815-21. 1993
    ..This result suggests that TSA is a physiologically important antioxidant...
  16. ncbi request reprint Regulator of G-protein signaling 3 (RGS3) inhibits Gbeta1gamma 2-induced inositol phosphate production, mitogen-activated protein kinase activation, and Akt activation
    C S Shi
    BCell Molecular Immunology Section, Laboratory of Immunoregulation, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:24293-300. 2001
    ..Thus, RGS3 may limit Gbetagamma signaling not only by virtue of its GTPase-activating protein activity for Galpha subunits, but also by directly interfering with the activation of effectors...
  17. pmc Cloning and sequencing of thiol-specific antioxidant from mammalian brain: alkyl hydroperoxide reductase and thiol-specific antioxidant define a large family of antioxidant enzymes
    H Z Chae
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 91:7017-21. 1994
    ..We also report that a second family of proteins, defined by the 57-kDa component (AhpF) of alkyl hydroperoxide reductase and by thioredoxin reductase, has expanded to include six additional members...
  18. ncbi request reprint Purification, molecular cloning, and sequencing of phospholipase C-beta 4
    C W Lee
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 268:21318-27. 1993
    ..These results suggest that the brain cDNA encodes PLC-beta 4, which is likely a mammalian homolog of PLC-norpA...
  19. ncbi request reprint Regulatory role for a novel human thioredoxin peroxidase in NF-kappaB activation
    D Y Jin
    Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 272:30952-61. 1997
    ..AOE372 function suggests thioredoxin peroxidase as an immediate regulator of H2O2-mediated activation of NF-kappaB...
  20. ncbi request reprint Regulation of phospholipase C isozymes: activation of phospholipase C-gamma in the absence of tyrosine-phosphorylation
    F Sekiya
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 0320, USA
    Chem Phys Lipids 98:3-11. 1999
    ..These results reveal an elaborate mechanism of cross-talk and mutual regulation between four effector enzymes that participate in receptor signaling by acting on phospholipids...
  21. ncbi request reprint PDGF stimulation of inositol phospholipid hydrolysis requires PLC-gamma 1 phosphorylation on tyrosine residues 783 and 1254
    H K Kim
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892
    Cell 65:435-41. 1991
    ..These results provide direct evidence that growth factor receptors activate the function of intracellular protein by tyrosine phosphorylation...
  22. ncbi request reprint Catalytic properties of inositol trisphosphate kinase: activation by Ca2+ and calmodulin
    S H Ryu
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892
    FASEB J 1:388-93. 1987
    ..Studies with various inositol polyphosphates indicate that the substrate-binding site is quite specific to Ins-1,4,5-P3. Nevertheless, Ins-2,4,5-P3 could be phosphorylated at a velocity approximately 1/20-1/30 that of Ins-1,4,5-P3...
  23. ncbi request reprint Molecular cloning and expression of a complementary DNA for inositol 1,4,5-trisphosphate 3-kinase
    K Y Choi
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892
    Science 248:64-6. 1990
    ..Expression of the cDNA in COS cells resulted in an approximately 150-fold increase in inositol 1,4,5-trisphosphate 3-kinase activity of these cells...
  24. ncbi request reprint Hydrogen peroxide: a key messenger that modulates protein phosphorylation through cysteine oxidation
    S G Rhee
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Sci STKE 2000:pe1. 2000
    ..Thus, the higher levels of intracellular phosphoproteins observed in cells most likely occur because of the concomitant inhibition of protein phosphatases and activation of protein kinases...
  25. ncbi request reprint Thioredoxin-dependent peroxide reductase from yeast
    H Z Chae
    Laboratory of Biochemistry, NHLBI, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 269:27670-8. 1994
    ..The Saccharomyces cerevisiae thioredoxin reductase gene was also cloned and sequenced, and the deduced amino sequence was shown to be 51% identical with that of the Escherichia coli enzyme...
  26. pmc Inositol phospholipid-specific phospholipase C: complete cDNA and protein sequences and sequence homology to tyrosine kinase-related oncogene products
    P G Suh
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 85:5419-23. 1988
    ..Therefore, this unexpected similarity raises the possibility that the 148-kDa phospholipase C and cytoplasmic tyrosine kinases are modulated by common cellular component(s)...
  27. ncbi request reprint Cloning, sequencing, expression, and Gq-independent activation of phospholipase C-beta 2
    D Park
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 267:16048-55. 1992
    ..see also accompanying article (Lee, C. H., Park, D., Wu, D., Rhee, S. G., and Simon, M. I. (1992) J. Biol. Chem. 267, 16044-16047)...
  28. ncbi request reprint Molecular cloning, splice variants, expression, and purification of phospholipase C-delta 4
    S B Lee
    Laboratory of Cell Signaling, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 271:25-31. 1996
    ..Splice variants have not previously been detected for delta-type PLC isozymes...
  29. ncbi request reprint Molecular cloning and characterization of a mitochondrial selenocysteine-containing thioredoxin reductase from rat liver
    S R Lee
    Laboratory of Cell Signaling, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 274:4722-34. 1999
    ....
  30. ncbi request reprint Tyrosine residues in bovine phospholipase C-gamma phosphorylated by the epidermal growth factor receptor in vitro
    J W Kim
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 265:3940-3. 1990
    ..We propose, therefore, that the phosphorylation of PLC-gamma by EGF receptor kinase alters its interaction with putative inhibitory proteins and leads to its activation...
  31. ncbi request reprint Inhibition of phospholipase D by amphiphysins
    C Lee
    Laboratory of Cell Signaling, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 0320, USA
    J Biol Chem 275:18751-8. 2000
    ..Thus, the inhibition of PLD by amphiphysins, synaptojanin, and AP180 might play an important role in synaptic vesicle trafficking...
  32. ncbi request reprint Different ARF domains are required for the activation of cholera toxin and phospholipase D
    G F Zhang
    Pulmonary Critical Care Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 270:21-4. 1995
    ..It appears that the amino-terminal region of ARF1 is not critical for its action as a GTP-dependent activator of cholera toxin, whereas it is necessary for activation of the putative effector enzyme, PLD...
  33. pmc Phosphorylation of Nck in response to a variety of receptors, phorbol myristate acetate, and cyclic AMP
    D Park
    Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892
    Mol Cell Biol 12:5816-23. 1992
    ..These results suggest that Nck is a target for a variety of protein kinases that might modulate the postulated role of Nck as an adaptor for the physical and functional coordination of signalling proteins...
  34. ncbi request reprint Molecular mechanism of the reduction of cysteine sulfinic acid of peroxiredoxin to cysteine by mammalian sulfiredoxin
    Woojin Jeong
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 281:14400-7. 2006
    ..The disulfide-S-monoxide is further reduced through the oxidation of three thiol equivalents to complete the catalytic cycle and regenerate Prx-Cys-SH...
  35. ncbi request reprint The RING-H2-finger protein APC11 as a target of hydrogen peroxide
    Tong Shin Chang
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 37:521-30. 2004
    ..Inhibition of APC11 function by H(2)O(2) thus likely contributes to the delay in cell cycle progression through mitosis that is characteristic of cells subjected to oxidative stress...
  36. ncbi request reprint Peroxiredoxin III, a mitochondrion-specific peroxidase, regulates apoptotic signaling by mitochondria
    Tong Shin Chang
    Laboratory of Cell Signaling, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:41975-84. 2004
    ..Our results suggest that Prx III is a critical regulator of the abundance of mitochondrial H(2)O(2), which itself promotes apoptosis in cooperation with other mediators of apoptotic signaling...
  37. ncbi request reprint Characterization of mammalian sulfiredoxin and its reactivation of hyperoxidized peroxiredoxin through reduction of cysteine sulfinic acid in the active site to cysteine
    Tong Shin Chang
    Laboratory of Cell Signaling, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:50994-1001. 2004
    ..Finally, depletion of human Srx by RNA interference suggested that Srx is largely responsible for reduction of the Cys-SO2H of Prx in A549 human cells...
  38. ncbi request reprint Regulation of PDGF signalling and vascular remodelling by peroxiredoxin II
    Min Hee Choi
    Division of Molecular Life Sciences and the Center for Cell Signaling Research, Ewha Womans University, Seoul 120 750, Korea
    Nature 435:347-53. 2005
    ..These results demonstrate a localized role for endogenous H2O2 in PDGF signalling, and indicate a biological function of Prx II in cardiovascular disease...
  39. pmc Reversible oxidation and inactivation of the tumor suppressor PTEN in cells stimulated with peptide growth factors
    Jaeyul Kwon
    Laboratories of Cell Signaling and Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 8015, USA
    Proc Natl Acad Sci U S A 101:16419-24. 2004
    ..Furthermore, together with previous observations, our data indicate that peroxiredoxin likely participates in PIP3 signaling by modulating the local concentration of H2O2...
  40. pmc Intramolecular interaction between phosphorylated tyrosine-783 and the C-terminal Src homology 2 domain activates phospholipase C-gamma1
    Benoit Poulin
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 8015, USA
    Proc Natl Acad Sci U S A 102:4276-81. 2005
    ..Our results thus clarify the molecular mechanism of PLC-gamma1 activation, revealing the specific function of pY783 and the distinct roles of the two SH2 domains in this process...
  41. ncbi request reprint Cell signaling. H2O2, a necessary evil for cell signaling
    Sue Goo Rhee
    Institute of Molecular Life Science and Technology, Ewha Women s University, Seoul 120 750, South Korea
    Science 312:1882-3. 2006
  42. ncbi request reprint Structural basis of cellular redox regulation by human TRP14
    Joo Rang Woo
    Center for Cellular Switch Protein Structure and Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Euh eun dong, Yuseong Gu, Daejeon 305 806, Korea
    J Biol Chem 279:48120-5. 2004
    ..These structural differences in the active site suggest that TRP14 has evolved to regulate cellular redox signaling by recognizing a distinctive group of substrates that would complement the group of proteins regulated by Trx1...
  43. ncbi request reprint AHNAK-mediated activation of phospholipase C-gamma1 through protein kinase C
    In Hye Lee
    Division of Molecular Life Sciences, Center for Cell Signaling Research, Ewha Womans University, Seoul 120 750, Korea
    J Biol Chem 279:26645-53. 2004
    ..The secondary activation of PLC is likely because of the scaffolding activity of AHNAK, which is consistent with the role of 4 CRUs as a molecular linker between PLC-gamma and PKC-alpha...
  44. ncbi request reprint Mutagenesis and modeling of the peroxiredoxin (Prx) complex with the NMR structure of ATP-bound human sulfiredoxin implicate aspartate 187 of Prx I as the catalytic residue in ATP hydrolysis
    Duck Yeon Lee
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 0301, USA
    Biochemistry 45:15301-9. 2006
    ..The modeling and mutagenesis analyses strongly implicate Asp187 of Prx I as the catalytic residue responsible for ATP hydrolysis in the cysteinesulfinic acid reduction of Prx by hSrx...
  45. ncbi request reprint Identification and characterization of TRP14, a thioredoxin-related protein of 14 kDa. New insights into the specificity of thioredoxin function
    Woojin Jeong
    Laboratory of Cell Signaling, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:3142-50. 2004
    ..These results suggest that TRP14 and Trx1 might act on distinct substrate proteins...
  46. ncbi request reprint Oxidation of proteinaceous cysteine residues by dopamine-derived H2O2 in PC12 cells
    Jae Ryong Kim
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland 20892, USA
    Arch Biochem Biophys 397:414-23. 2002
    ....
  47. ncbi request reprint Roles of TRP14, a thioredoxin-related protein in tumor necrosis factor-alpha signaling pathways
    Woojin Jeong
    Laboratory of Cell Signaling, National Heart, Lung and Bllod Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:3151-9. 2004
    ..The complex was detected in HeLa cells treated with H(2)O(2) or TNF-alpha but not in untreated cells, suggesting that LC8 cytoplasmic dynein light chain is a possible substrate of TRP14...
  48. ncbi request reprint Regulation of peroxiredoxin I activity by Cdc2-mediated phosphorylation
    Tong Shin Chang
    Laboratory of Cell Signaling, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:25370-6. 2002
    ..These results suggest that Cdc2-mediated phosphorylation and inactivation of Prx I and the resulting intracellular accumulation of H(2)O(2) might be important for progression of the cell cycle...
  49. ncbi request reprint Reversible oxidation of the active site cysteine of peroxiredoxins to cysteine sulfinic acid. Immunoblot detection with antibodies specific for the hyperoxidized cysteine-containing sequence
    Hyun Ae Woo
    Center for Cell Signaling Research and Division of Molecular Life Sciences, Ewha Womans University, Seoul 120 750, Korea
    J Biol Chem 278:47361-4. 2003
    ..The generation of antibodies specific for sulfonylated peptides should provide insight into protein function similar to that achieved with antibodies to peptides containing phosphoserine or phosphothreonine...
  50. ncbi request reprint Inactivation of human peroxiredoxin I during catalysis as the result of the oxidation of the catalytic site cysteine to cysteine-sulfinic acid
    Kap Seok Yang
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:38029-36. 2002
    ..072% per turnover at 30 degrees C. Hyperoxidation of Prx I was also detected in HeLa cells treated with H(2)O(2)...
  51. ncbi request reprint Reversing the inactivation of peroxiredoxins caused by cysteine sulfinic acid formation
    Hyun Ae Woo
    Center for Cell Signaling Research and Division of Molecular Life Sciences, Ewha Womans University, Seoul 120 750, Korea
    Science 300:653-6. 2003
    ..The mammalian cells' ability to reduce protein sulfinic acid might serve as a mechanism to repair oxidatively damaged proteins or represent a new type of cyclic modification by which the function of various proteins is regulated...
  52. doi request reprint Ahnak protein activates protein kinase C (PKC) through dissociation of the PKC-protein phosphatase 2A complex
    In Hye Lee
    Department of Life Science, Ewha Womans University, 11 1 Daehyun Dong, Seodaemoon Gu, Seoul 120 750, Korea
    J Biol Chem 283:6312-20. 2008
    ..These data indicate that Ahnak potentiates PKC activation through inhibiting the interaction of PKC with PP2A...
  53. ncbi request reprint Laminar shear stress up-regulates peroxiredoxins (PRX) in endothelial cells: PRX 1 as a mechanosensitive antioxidant
    Amy L Mowbray
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30322, USA
    J Biol Chem 283:1622-7. 2008
    ..However, PRX 5 depletion did not. Together, these results suggest that PRX 1 is a novel mechanosensitive antioxidant, playing an important role in shear-dependent regulation of endothelial biology and atherosclerosis...
  54. ncbi request reprint Measuring H2O2 produced in response to cell surface receptor activation
    Sue Goo Rhee
    Nat Chem Biol 3:244-6. 2007
  55. ncbi request reprint 1H, 15N, and 13C chemical shift assignments of the human Sulfiredoxin (hSrx)
    Duck Yeon Lee
    J Biomol NMR 32:339. 2005
  56. ncbi request reprint 2-Cys peroxiredoxin function in intracellular signal transduction: therapeutic implications
    Sang Won Kang
    Center for Cell Signaling Research and Division of Molecular Life Science, Ewha Womans University, Seoul 120 750, Korea
    Trends Mol Med 11:571-8. 2005
    ..Thus, the therapeutic potential of 2-Cys peroxiredoxins is clear for diseases, such as cancer and cardiovascular diseases, that involve reactive oxygen species...
  57. pmc ERp16, an endoplasmic reticulum-resident thiol-disulfide oxidoreductase: biochemical properties and role in apoptosis induced by endoplasmic reticulum stress
    Woojin Jeong
    Division of Life and Pharmaceutical Sciences, Ewha Womans University, 11 1 Daehyun Dong, Seodaemun gu, Seoul, Korea
    J Biol Chem 283:25557-66. 2008
    ..Our results suggest that ERp16 mediates disulfide bond formation in the ER and plays an important role in cellular defense against prolonged ER stress...
  58. ncbi request reprint Annexin A2-S100A10 heterotetramer, a novel substrate of thioredoxin
    Mijung Kwon
    Cancer Biology Research Group, Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    J Biol Chem 280:23584-92. 2005
    ..Therefore, we identify AIIt as a substrate of the thioredoxin system and propose a new model for the role of AIIt in the redox-dependent processing of plasminogen and generation of an angiostatin at the cell surface...
  59. ncbi request reprint Activity assay of mammalian 2-cys peroxiredoxins using yeast thioredoxin reductase system
    Ju A Kim
    Center for Cell Signaling Research and Division of Molecular Life Sciences, Ewha Woman s University, Seoul, Korea
    Anal Biochem 338:216-23. 2005
    ..5 microM, respectively. This modified assay method is useful for measuring 2-cys Prx activity in cell lysates and can also be adapted for a 96-well plate reader for high-throughput screening of chemical compounds that target 2-cys Prx...
  60. ncbi request reprint Inositol lipid binding and membrane localization of isolated pleckstrin homology (PH) domains. Studies on the PH domains of phospholipase C delta 1 and p130
    Peter Varnai
    Endocrinology and Reproduction Research Branch, NICHD National Institutes of Health, 49 Convent Drive, Bldg 49, Bethesda, MD 20892, USA
    J Biol Chem 277:27412-22. 2002
    ....
  61. ncbi request reprint Peroxiredoxin II is essential for sustaining life span of erythrocytes in mice
    Tae Hoon Lee
    Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea
    Blood 101:5033-8. 2003
    ..Our results suggest that Prx II-/- mice have hemolytic anemia and that Prx II plays a major role in protecting RBCs from oxidative stress in mice...
  62. pmc Discrete generation of superoxide and hydrogen peroxide by T cell receptor stimulation: selective regulation of mitogen-activated protein kinase activation and fas ligand expression
    Satish Devadas
    Department of Immunology, Holland Laboratory, American Red Cross, Rockville, MD 20855, USA
    J Exp Med 195:59-70. 2002
    ..Thus, antigen receptor signaling induces generation of discrete species of oxidants that selectively regulate two distinct redox sensitive pathways, a proapoptotic (FasL) and a proliferative pathway (ERK)...
  63. ncbi request reprint Cytosolic peroxiredoxin attenuates the activation of Jnk and p38 but potentiates that of Erk in Hela cells stimulated with tumor necrosis factor-alpha
    Sang Won Kang
    Laboratory of Cell Signaling, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:2535-43. 2004
    ..Our results also suggest that cytosolic peroxiredoxins are important regulators of TNF signaling pathways...
  64. pmc Glycoproteins VI and Ib-IX-V stimulate tyrosine phosphorylation of tyrosine kinase Syk and phospholipase Cgamma2 at distinct sites
    Katsue Suzuki-Inoue
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK
    Biochem J 378:1023-9. 2004
    ..Our results demonstrate fundamental differences between GPVI and GPIb-IX-V in the regulation of tyrosine phosphorylation of Syk and PLCg2 consistent with the functional impairment of phospholipase in signalling by GPIb-IX-V...
  65. pmc Sequential activation of phosphatidylinositol 3-kinase, beta Pix, Rac1, and Nox1 in growth factor-induced production of H2O2
    Hye Sun Park
    Center for Cell Signaling Research, Division of Molecular Life Sciences, Ewha Womans University, Seoul 120 750, Korea
    Mol Cell Biol 24:4384-94. 2004
    ..These results suggest that ROS production in growth factor-stimulated cells is mediated by the sequential activation of PI3K, beta Pix, and Rac1, which then binds to Nox1 to stimulate its NADPH oxidase activity...
  66. ncbi request reprint Mechanism of tyrosine phosphorylation and activation of phospholipase C-gamma 1. Tyrosine 783 phosphorylation is not sufficient for lipase activation
    Fujio Sekiya
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:32181-90. 2004
    ..Finally, concurrent production of phosphatidylinositol 3,4,5-trisphosphate was found to contribute to the activation of phosphorylated PLC-gamma 1...
  67. ncbi request reprint Two enzymes in one; two yeast peroxiredoxins display oxidative stress-dependent switching from a peroxidase to a molecular chaperone function
    Ho Hee Jang
    Division of Applied Life Sciences, Gyeonsang National University, Chinju, 660 701, South Korea
    Cell 117:625-35. 2004
    ..The chaperone function of these proteins enhances yeast resistance to heat shock...
  68. ncbi request reprint Identification of phospholipase C-gamma1 as a mitogen-activated protein kinase substrate
    Colin T Buckley
    Department of Neurosciences, University of New Mexico Health Sciences Center, Albuquerque, New Mexico 87131, USA
    J Biol Chem 279:41807-14. 2004
    ..These studies have uncovered a previously unidentified mechanism for the integration of PLC-gamma1- and ERK2-dependent signaling...
  69. ncbi request reprint Phospholipase C-delta1 rescues intracellular Ca2+ overload in ischemic heart and hypoxic neonatal cardiomyocytes
    Ki Chul Hwang
    Department of Internal Medicine, Cardiovascular Research Institute, Yonsei University College of Medicine, Yonsei University, Seoul 120 752, South Korea
    J Steroid Biochem Mol Biol 91:131-8. 2004
    ..It is suggested that PLC isozyme-changes may contribute to the alterations in calcium homeostasis in myocardial ischemia...
  70. pmc Mechanism of B-cell receptor-induced phosphorylation and activation of phospholipase C-gamma2
    Yeun Ju Kim
    Laboratory of Cell Signaling, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Mol Cell Biol 24:9986-99. 2004
    ..Finally, phosphorylation of Y1217 and that of Y753 and Y759 occurred on different PLC-gamma2 molecules...
  71. ncbi request reprint Membrane targeting of C2 domains of phospholipase C-delta isoforms
    Bharath Ananthanarayanan
    Department of Chemistry, University of Illinois, Chicago 60607, USA
    J Biol Chem 277:3568-75. 2002
    ..Together, these results establish the C2 domains of PLC-delta isoforms as Ca(2+)-dependent membrane targeting domains that have distinct membrane binding properties that control their subcellular localization behaviors...