T Michael Redmond

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi Expression and promoter activation of the Rpe65 gene in retinal pigment epithelium cell lines
    Ana Boulanger
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Eye Res 24:368-75. 2002
  2. ncbi Mole quantity of RPE65 and its productivity in the generation of 11-cis-retinal from retinyl esters in the living mouse eye
    Arkady L Lyubarsky
    F M Kirby Center for Molecular Ophthalmology, Department of Ophthalmology, School of Medicine, University of Pennsylvania, 422 Curie Boulevard, Philadelphia, Pennsylvania 19104 6069, USA
    Biochemistry 44:9880-8. 2005
  3. ncbi RPE65, visual cycle retinol isomerase, is not inherently 11-cis-specific: support for a carbocation mechanism of retinol isomerization
    T Michael Redmond
    Laboratory of Retinal Cell and Molecular Biology, NEI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 285:1919-27. 2010
  4. ncbi Identification, expression, and substrate specificity of a mammalian beta-carotene 15,15'-dioxygenase
    T M Redmond
    Laboratory of Retinal Cell and Molecular Biology, NEI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:6560-5. 2001
  5. ncbi Effect of Leu/Met variation at residue 450 on isomerase activity and protein expression of RPE65 and its modulation by variation at other residues
    T Michael Redmond
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, NIH, Bethesda, MD 20892 0706, USA
    Mol Vis 13:1813-21. 2007
  6. ncbi Mutation of key residues of RPE65 abolishes its enzymatic role as isomerohydrolase in the visual cycle
    T Michael Redmond
    Laboratory of Retinal Cell and Molecular Biology and Biological Imaging Core, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 0706, USA
    Proc Natl Acad Sci U S A 102:13658-63. 2005
  7. ncbi Rpe65 is necessary for production of 11-cis-vitamin A in the retinal visual cycle
    T M Redmond
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Genet 20:344-51. 1998
  8. ncbi Decreased expression of insulin-like growth factor binding protein-5 during N-(4-hydroxyphenyl)retinamide-induced neuronal differentiation of ARPE-19 human retinal pigment epithelial cells: regulation by CCAAT/enhancer-binding protein
    William Samuel
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 0706, USA
    J Cell Physiol 224:827-36. 2010
  9. ncbi Key role of conserved histidines in recombinant mouse beta-carotene 15,15'-monooxygenase-1 activity
    Eugenia Poliakov
    Laboratory of Retinal Cell and Molecular Biology, NEI, National Institutes of Health, Bethesda, MD 20892 0706, USA
    J Biol Chem 280:29217-23. 2005
  10. ncbi Biochemical evidence for the tyrosine involvement in cationic intermediate stabilization in mouse beta-carotene 15, 15'-monooxygenase
    Eugenia Poliakov
    National Eye Institute, NIH, Bethesda, MD 20892 0608, USA
    BMC Biochem 10:31. 2009

Collaborators

Detail Information

Publications46

  1. ncbi Expression and promoter activation of the Rpe65 gene in retinal pigment epithelium cell lines
    Ana Boulanger
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Eye Res 24:368-75. 2002
    ..This limits the study of the transcriptional regulation of the mouse Rpe65 gene in vitro to this particular cell line...
  2. ncbi Mole quantity of RPE65 and its productivity in the generation of 11-cis-retinal from retinyl esters in the living mouse eye
    Arkady L Lyubarsky
    F M Kirby Center for Molecular Ophthalmology, Department of Ophthalmology, School of Medicine, University of Pennsylvania, 422 Curie Boulevard, Philadelphia, Pennsylvania 19104 6069, USA
    Biochemistry 44:9880-8. 2005
    ....
  3. ncbi RPE65, visual cycle retinol isomerase, is not inherently 11-cis-specific: support for a carbocation mechanism of retinol isomerization
    T Michael Redmond
    Laboratory of Retinal Cell and Molecular Biology, NEI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 285:1919-27. 2010
    ..Specific visual cycle selectivity for 11-cis isomers instead resides downstream, attributable to mass action by CRALBP, retinol dehydrogenase 5, and high affinity of opsin apoproteins for 11-cis-retinal...
  4. ncbi Identification, expression, and substrate specificity of a mammalian beta-carotene 15,15'-dioxygenase
    T M Redmond
    Laboratory of Retinal Cell and Molecular Biology, NEI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:6560-5. 2001
    ..Identification of beta-CD in an accessible model organism will create new opportunities to study vitamin A metabolism...
  5. ncbi Effect of Leu/Met variation at residue 450 on isomerase activity and protein expression of RPE65 and its modulation by variation at other residues
    T Michael Redmond
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, NIH, Bethesda, MD 20892 0706, USA
    Mol Vis 13:1813-21. 2007
    ..We wished to determine how this variant affects RPE65 and if it is modulated by other rodent-specific variations...
  6. ncbi Mutation of key residues of RPE65 abolishes its enzymatic role as isomerohydrolase in the visual cycle
    T Michael Redmond
    Laboratory of Retinal Cell and Molecular Biology and Biological Imaging Core, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 0706, USA
    Proc Natl Acad Sci U S A 102:13658-63. 2005
    ..These findings establish a catalytic role, in conjunction with lecithin:retinol acyltransferase, for RPE65 in synthesis of 11-cis-retinol, and its identity as the isomerohydrolase...
  7. ncbi Rpe65 is necessary for production of 11-cis-vitamin A in the retinal visual cycle
    T M Redmond
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Genet 20:344-51. 1998
    ..Disruption of the RPE-based metabolism of all-trans-retinyl esters to 11-cis-retinal thus appears to underlie the Rpe65-/- phenotype, although cone pigment regeneration may be dependent on a separate pathway...
  8. ncbi Decreased expression of insulin-like growth factor binding protein-5 during N-(4-hydroxyphenyl)retinamide-induced neuronal differentiation of ARPE-19 human retinal pigment epithelial cells: regulation by CCAAT/enhancer-binding protein
    William Samuel
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 0706, USA
    J Cell Physiol 224:827-36. 2010
    ..Thus, our results clearly demonstrate that the IGFBP5 expression is down-regulated during 4HPR-induced neuronal differentiation of human RPE cells through a MAPK signal transduction pathway involving C/EBPbeta...
  9. ncbi Key role of conserved histidines in recombinant mouse beta-carotene 15,15'-monooxygenase-1 activity
    Eugenia Poliakov
    Laboratory of Retinal Cell and Molecular Biology, NEI, National Institutes of Health, Bethesda, MD 20892 0706, USA
    J Biol Chem 280:29217-23. 2005
    ..These data are discussed in the context of the predicted structure for the related eubacterial apocarotenal oxygenase...
  10. ncbi Biochemical evidence for the tyrosine involvement in cationic intermediate stabilization in mouse beta-carotene 15, 15'-monooxygenase
    Eugenia Poliakov
    National Eye Institute, NIH, Bethesda, MD 20892 0608, USA
    BMC Biochem 10:31. 2009
    ..Enzymatic activity was measured in vitro using His-tag purified proteins and in vivo in a beta-carotene-accumulating E. coli system...
  11. ncbi MicroRNA expression in human retinal pigment epithelial (ARPE-19) cells: increased expression of microRNA-9 by N-(4-hydroxyphenyl)retinamide
    R Krishnan Kutty
    Laboratory of Retinal Cell and Molecular Biology, Bldg 6, Room 112, National Eye Institute, National Institutes of Health, 6 Center Dr, MSC 0608, Bethesda, MD 20892, USA
    Mol Vis 16:1475-86. 2010
    ..The aim of the present study was to investigate the expression of miR-9 in ARPE-19 cells in response to 4HPR treatment, and to identify other miRNAs normally expressed in these cells...
  12. ncbi Aromatic residues in the substrate cleft of RPE65 protein govern retinol isomerization and modulate its progression
    Preethi Chander
    Laboratory of Retinal Cell and Molecular Biology, NEI, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 287:30552-9. 2012
    ..These findings provide insight into the mechanism of isomerization central to the visual cycle...
  13. ncbi Expression of beta-carotene 15,15' monooxygenase in retina and RPE-choroid
    Rizwan A Bhatti
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, 6 Center Drive, Building 6 Room 339, Bethesda, MD 20892, USA
    Invest Ophthalmol Vis Sci 44:44-9. 2003
    ..Given the low and variable expression of beta-CM in the retina and RPE, it can be concluded that beta-CM is not necessary for a conserved retina or RPE-specific function, but may be necessary for a species-specific function...
  14. ncbi Differential regulation of microRNA-146a and microRNA-146b-5p in human retinal pigment epithelial cells by interleukin-1β, tumor necrosis factor-α, and interferon-γ
    R Krishnan Kutty
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD
    Mol Vis 19:737-50. 2013
    ..The aim of the present study is to investigate the expression of miR-146a and miR-146b-5p in human RPE cells and their response to proinflammatory cytokines...
  15. ncbi Identification of a KRAB-zinc finger protein binding to the Rpe65 gene promoter
    Zhongjian Lu
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute NIH, 7 Memorial Drive, Bethesda, MD 20892, USA
    Curr Eye Res 31:457-66. 2006
    ..We wish to identify transcriptional factors involved in regulation binding to the proximal promoter region of the RPE65 gene that confers RPE-specific expression...
  16. ncbi Aromatic lipophilic spin traps effectively inhibit RPE65 isomerohydrolase activity
    Eugenia Poliakov
    LRCMB, NEI, National Institutes of Health, Bethesda, Maryland 20892, United States
    Biochemistry 50:6739-41. 2011
    ..We found that the aromatic lipophilic spin traps such as N-tert-butyl-α-phenylnitrone (PBN), 2,2-dimethyl-4-phenyl-2H-imidazole-1-oxide (DMPIO), and nitrosobenzene (NB) strongly inhibit RPE65 isomerohydrolase activity in vitro...
  17. ncbi The upstream region of the Rpe65 gene confers retinal pigment epithelium-specific expression in vivo and in vitro and contains critical octamer and E-box binding sites
    A Boulanger
    Laboratory of Retinal Cell and Molecular Biology, NEI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 275:31274-82. 2000
    ....
  18. ncbi Mitogen-activated protein kinase pathway mediates N-(4-hydroxyphenyl)retinamide-induced neuronal differentiation in the ARPE-19 human retinal pigment epithelial cell line
    William Samuel
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, Bethesda, Maryland, USA
    J Neurochem 106:591-602. 2008
    ....
  19. ncbi Identification of beta-carotene 15, 15'-monooxygenase as a peroxisome proliferator-activated receptor target gene
    Ana Boulanger
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 2740, USA
    FASEB J 17:1304-6. 2003
    ....
  20. ncbi The gene for the retinal pigment epithelium-specific protein RPE65 is localized to human 1p31 and mouse 3
    C P Hamel
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892
    Genomics 20:509-12. 1994
    ..By the use of fluorescence in situ hybridization, this localization was refined to 1p31. The mouse and human loci for this potential candidate gene for hereditary retinal disease do not match those of any known disease in mouse or man...
  21. ncbi Molecular cloning and expression of RPE65, a novel retinal pigment epithelium-specific microsomal protein that is post-transcriptionally regulated in vitro
    C P Hamel
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 268:15751-7. 1993
    ..Such cells, however, contained no immunodetectable RPE65. The possible structure of this RPE-specific protein and hypotheses for the absence of translation in vitro are discussed...
  22. ncbi Sequence and structure of the mouse gene for RPE65
    A Boulanger
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2740, USA
    Mol Vis 7:283-7. 2001
    ..To determine the genomic organization of the mouse gene for the retinal pigment epithelium (RPE) specific protein RPE65...
  23. ncbi RPE65 is highly uveitogenic in rats
    Don-Il Ham
    Laboratory of Immunology, National Eye Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 10N112, Bethesda, MD 20892-1857, USA
    Invest Ophthalmol Vis Sci 43:2258-63. 2002
    ..CONCLUSIONS: RPE65 is highly uveitogenic in rats, thus suggesting that this molecule could be involved in pathogenic autoimmunity in the human eye...
  24. ncbi Inflammatory cytokines regulate microRNA-155 expression in human retinal pigment epithelial cells by activating JAK/STAT pathway
    R Krishnan Kutty
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Biochem Biophys Res Commun 402:390-5. 2010
    ..All these activities were effectively blocked by JAK inhibitor 1. Our results show that the inflammatory cytokines increase miR-155 expression in human retinal pigment epithelial cells by activating the JAK/STAT signaling pathway...
  25. ncbi Synthesis of an immunopathogenic fusion protein derived from a bovine interphotoreceptor retinoid-binding protein cDNA clone
    T M Redmond
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, Bethesda, MD 20892
    Gene 80:109-18. 1989
    ..Thus, it is evident that biological activity of this region of IRBP, as manifested by immuno-pathogenicity, is retained by the fusion protein...
  26. ncbi Origin and evolution of retinoid isomerization machinery in vertebrate visual cycle: hint from jawless vertebrates
    Eugenia Poliakov
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD, USA
    PLoS ONE 7:e49975. 2012
    ..in the last common ancestor of the jawless and jawed vertebrates...
  27. ncbi Cloning of cDNAs encoding human interphotoreceptor retinoid-binding protein (IRBP) and comparison with bovine IRBP sequences
    J S Si
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, Bethesda, MD 20892
    Gene 80:99-108. 1989
    ..Thus, the human sequence is virtually full length, is similar to the bovine sequence, and contains a striking fourfold repeat...
  28. ncbi RPE65, the major retinal pigment epithelium microsomal membrane protein, associates with phospholipid liposomes
    E Tsilou
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Arch Biochem Biophys 346:21-7. 1997
    ..This RPE65-phospholipid liposome association may explain the solubilization characteristics of RPE65 and may be related to the function of RPE65 and to its physical association with the RPE smooth endoplasmic reticulum...
  29. ncbi A developmentally regulated microsomal protein specific for the pigment epithelium of the vertebrate retina
    C P Hamel
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, NIH, Bethesda, Maryland 20892
    J Neurosci Res 34:414-25. 1993
    ....
  30. ncbi Interphotoreceptor retinoid-binding protein. Gene characterization, protein repeat structure, and its evolution
    D E Borst
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, Bethesda, Maryland 20892
    J Biol Chem 264:1115-23. 1989
    ..The human IRBP gene has a sequence that is similar to one of the introns from the bovine gene. The unexpected gene structure and protein repeat structure in the bovine gene lead us to propose a model for the evolution of the IRBP gene...
  31. ncbi Transgenic expression of an immunologically privileged retinal antigen extraocularly enhances self tolerance and abrogates susceptibility to autoimmune uveitis
    H Xu
    NEI NIH, Bethesda, USA
    Eur J Immunol 30:272-8. 2000
    ..The same level of expression is, however, insufficient to tolerize wild-type effector T cells in the periphery...
  32. ncbi Safety and efficacy of gene transfer for Leber's congenital amaurosis
    Albert M Maguire
    Scheie Eye Institute, University of Pennsylvania, USA
    N Engl J Med 358:2240-8. 2008
    ..Although the follow-up was very short and normal vision was not achieved, this study provides the basis for further gene therapy studies in patients with LCA...
  33. ncbi Correlation of regenerable opsin with rod ERG signal in Rpe65-/- mice during development and aging
    Baerbel Rohrer
    Department of Ophthalmology Medical University of South Carolina, 167 Ashley Avenue, Charleston, SC 29425, USA
    Invest Ophthalmol Vis Sci 44:310-5. 2003
    ..This project was designed to determine the amount of regenerable opsin in Rpe65-/- mice during development and aging, and to examine the function of this rhodopsin by electroretinography (ERG)...
  34. ncbi 11-cis-retinal reduces constitutive opsin phosphorylation and improves quantum catch in retinoid-deficient mouse rod photoreceptors
    Zsolt Ablonczy
    Department of Ophthalmology, Medical University of South Carolina, 167 Ashley Avenue, Charleston, SC 29425, USA
    J Biol Chem 277:40491-8. 2002
    ..Our results indicate that opsin, which has not been exposed to 11-cis-retinal, does not generate the activity generally associated with the bleached apoprotein...
  35. ncbi Impairment of the transient pupillary light reflex in Rpe65(-/-) mice and humans with leber congenital amaurosis
    Tomas S Aleman
    Department of Ophthalmology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Invest Ophthalmol Vis Sci 45:1259-71. 2004
    ..To determine the impairment of the transient pupillary light reflex (TPLR) due to severe retinal dysfunction and degeneration in a murine model of Leber congenital amaurosis (LCA) and in patients with the disease...
  36. ncbi Retinyl esters are the substrate for isomerohydrolase
    Gennadiy Moiseyev
    Department of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Biochemistry 42:2229-38. 2003
    ..g., at 36 weeks, the levels are 20x that of WT). Our data indicate that the retinyl esters are the substrate of the isomerization reaction...
  37. ncbi Functional and structural recovery of the retina after gene therapy in the RPE65 null mutation dog
    Kristina Narfstrom
    Vision Science Group, Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Invest Ophthalmol Vis Sci 44:1663-72. 2003
    ..To assess the efficacy of AAV-mediated gene therapy to restore vision in a large number of RPE65(-/-) dogs and to determine whether systemic and local side effects are caused by the treatment...
  38. ncbi Assessment of rAAV-mediated gene therapy in the Rpe65-/- mouse
    P Elizabeth Rakoczy
    Centre for Ophthalmology and Visual Science, University of Western Australia, Perth, Western Australia, Australia
    Adv Exp Med Biol 533:431-8. 2003
  39. ncbi In utero gene therapy rescues vision in a murine model of congenital blindness
    Nadine S Dejneka
    F.M. Kirby Center and Scheie Eye Institute, Department of Ophthalmology, University of Pennsylvania, 51 N. 39th Street, Philadelphia, PA 19104-2689, USA
    Mol Ther 9:182-8. 2004
    ..The results demonstrate AAV-mediated correction of the deficit and suggest that in utero retinal gene delivery may be a useful approach for treating a variety of blinding congenital retinal diseases...
  40. ncbi Acute radiolabeling of retinoids in eye tissues of normal and rpe65-deficient mice
    Nasser M Qtaishat
    Lions of Illinois Eye Research Institute, Department of Ophthalmology and Visual Sciences, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612, USA
    Invest Ophthalmol Vis Sci 44:1435-46. 2003
    ..The present study was undertaken to determine whether the Rpe65-deficient mouse exhibits an abnormal flux of retinoid between the systemic circulation and the eye tissues...
  41. ncbi Cone opsin mislocalization in Rpe65-/- mice: a defect that can be corrected by 11-cis retinal
    Baerbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, Charleston, 29425, USA
    Invest Ophthalmol Vis Sci 46:3876-82. 2005
    ..This study was designed to investigate the feasibility of restoring functional cones with exogenous 11-cis retinal...
  42. ncbi RPE65 is an iron(II)-dependent isomerohydrolase in the retinoid visual cycle
    Gennadiy Moiseyev
    Department of Cell Biology, Department of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 281:2835-40. 2006
    ..Inductively coupled plasma mass spectrometry measurements showed that bovine RPE65 binds iron ion with a stoichiometry of 0.8 +/- 0.1. These results indicate that RPE65 is an iron-dependent isomerohydrolase in the visual cycle...
  43. ncbi 9-cis Retinal increased in retina of RPE65 knockout mice with decrease in coat pigmentation
    Jie Fan
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC, USA
    Photochem Photobiol 82:1461-7. 2006
    ..Therefore, photoreceptor damage correlates with the amount of the apoprotein present, supporting findings that the activity from unregenerated opsin can lead to photoreceptor degeneration...
  44. ncbi Spontaneous activity of opsin apoprotein is a cause of Leber congenital amaurosis
    Michael L Woodruff
    Department of Physiological Science, University of California Los Angeles, Los Angeles, California 90095, USA
    Nat Genet 35:158-64. 2003
    ..A similar mechanism may also be responsible for degeneration induced by vitamin A deprivation...
  45. ncbi Assessing the efficacy of gene therapy in Rpe65-/- mice using photoentrainment of circadian rhythm
    Chris W Stoddart
    Centre for Ophthalmology and Visual Science, The University of Western Australia, Perth, Western Australia
    Adv Exp Med Biol 572:239-45. 2006
  46. ncbi Functional and structural evaluation after AAV.RPE65 gene transfer in the canine model of Leber's congenital amaurosis
    Kristina Narfstrom
    University of Missouri Columbia, MO, USA
    Adv Exp Med Biol 533:423-30. 2003