Affiliation: National Cancer Institute
- Fluorescent lipid probes in the study of viral membrane fusionRobert Blumenthal
Laboratory of Experimental and Computational Biology, Center for Cancer Research, SAIC, P O Box B, Bldg 469, Rm 216A, Miller Drive, NCI Frederick, MD 21702 1201, USA
Chem Phys Lipids 116:39-55. 2002..Thus, fluorescent lipid probes provide a broad repertoire of fusion assays and powerful tools to produce precise, quantitative data in real time required for the elucidation of the complex process of viral fusion...
- Ebola virus inactivation with preservation of antigenic and structural integrity by a photoinducible alkylating agentKelly L Warfield
US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA
J Infect Dis 196:S276-83. 2007..Thus, INA may have significant potential for the development of vaccines and immunotherapeutics for filoviruses and other enveloped viruses...
- Hydrophobic inactivation of influenza viruses confers preservation of viral structure with enhanced immunogenicityYossef Raviv
Nanobiology Program, Center of Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA
J Virol 82:4612-9. 2008..When used as a vaccine given intranasally (i.n.), INA-inactivated influenza virus induced immune responses similar to live virus infection...
- Inactivation of retroviruses with preservation of structural integrity by targeting the hydrophobic domain of the viral envelopeYossef Raviv
Center for Cancer Research Nanobiology Program, NCI Frederick, Frederick, MD 21702, USA
J Virol 79:12394-400. 2005..Targeting the lipid domain of biological membranes with hydrophobic alkylating compounds could be used as a general approach for inactivation of enveloped viruses and other pathogenic microorganisms for vaccine application...
- HIV-1 envelope glycoprotein-mediated fusion and pathogenesis: implications for therapy and vaccine developmentAmy Jacobs
Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA
Vaccine 26:3026-35. 2008..Taken as a whole, our studies have many different important implications for antiviral therapies and vaccine development...
- Orthogonal inactivation of influenza and the creation of detergent resistant viral aggregates: towards a novel vaccine strategyJulie M Belanger
Center for Cancer Research Nanobiology Program, National Cancer Institute Frederick, Frederick, USA
Virol J 9:72. 2012..Prolonged UVA irradiation in the presence of INA results in ROS-species formation, which in turn results in detergent resistant viral protein fractions...
- Effects of UVA irradiation, aryl azides, and reactive oxygen species on the orthogonal inactivation of the human immunodeficiency virus (HIV-1)Julie M Belanger
Center for Cancer Research Nanobiology Program, National Cancer Institute Frederick, Frederick, MD 21702, USA
Virology 417:221-8. 2011....
- The HIV Env-mediated fusion reactionStephen A Gallo
Laboratory of Experimental and Computational Biology, Center for Cancer Research, NCI Frederick, National Institute of Health, Miller Drive, Frederick, MD 21702 1201, USA
Biochim Biophys Acta 1614:36-50. 2003..A review of these data that may require an updated version of the original model is presented here...
- Photo-activation of the hydrophobic probe iodonaphthylazide in cells alters membrane protein function leading to cell deathMathias Viard
Nanobiology Program, Center of Cancer Research, National Cancer Institute, Frederick, Maryland, USA
BMC Cell Biol 10:21. 2009..The effects of INA-UV treatment on signaling via various cell surface receptors, on the activity of the multi-drug resistance transporter MRP1 and on membrane protein lateral mobility were also investigated...
- Ectopic ATP synthase facilitates transfer of HIV-1 from antigen-presenting cells to CD4(+) target cellsAmichai Yavlovich
Membrane Structure and Function Section, Nanobiology Program, Center for Cancer Research, Bethesda, MD, USA
Blood 120:1246-53. 2012..We conclude that ectopic ATP synthase could be an accessible molecular target for inhibiting HIV-1 proliferation in vivo...
- Characterization of the effects of aryl-azido compounds and UVA irradiation on the viral proteins and infectivity of human immunodeficiency virus type 1Julie M Belanger
Center for Cancer Research Nanobiology Program, NCI Frederick, Frederick, MD, USA
Photochem Photobiol 86:1099-108. 2010....
- Photoinduced reactivity of the HIV-1 envelope glycoprotein with a membrane-embedded probe reveals insertion of portions of the HIV-1 Gp41 cytoplasmic tail into the viral membraneMathias Viard
Center of Cancer Research Nanobiology Program, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA
Biochemistry 47:1977-83. 2008..These results shed light on the disposition of the HIV-1 gp41 CT with respect to the membrane. Moreover, our data have general implications for topology of membrane proteins and their in situ interactions with the lipid bilayer...
- Quantitative measurement of fusion of HIV-1 and SIV with cultured cells using photosensitized labelingYossef Raviv
Laboratory of Experimental and Computational Biology, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA
Virology 293:243-51. 2002..This feature provides a basis for understanding the mode of action of HIV/SIV entry inhibitors that target transition states...
- Improved separation of integral membrane proteins by continuous elution electrophoresis with simultaneous detergent exchange: application to the purification of the fusion protein of the human immunodeficiency virus type 1Mathias Viard
Laboratory of Experimental and Computational Biology, Center for Cancer Research, NCI Frederick, Bldg 469, Rm 213, Miller Drive, Frederick, MD 21702, USA
Electrophoresis 23:1659-66. 2002..The possible application of this approach for the isolation of integral membrane proteins with the propensity to undergo spontaneous folding and aggregation is being discussed...
- Complete inactivation of Venezuelan equine encephalitis virus by 1,5-iodonaphthylazideAnuj Sharma
Centre for Combat Casualty and Life Sustainment Research, Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
Biochem Biophys Res Commun 358:392-8. 2007..This data suggest that such inactivation strategies can be used for developing vaccine candidates for VEEV and other enveloped viruses...
- Identification of PhIL1, a novel cytoskeletal protein of the Toxoplasma gondii pellicle, through photosensitized labeling with 5-[125I]iodonaphthalene-1-azideStacey D Gilk
Department of Microbiology and Molecular Genetics, 316 Stafford Hall, University of Vermont, Burlington, VT 05405, USA
Eukaryot Cell 5:1622-34. 2006..Detergent extraction and immunolocalization data suggest that PhIL1 associates with the parasite cytoskeleton...