Mahendra Rao

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi Are there morally acceptable alternatives to blastocyst derived ESC?
    Mahendra S Rao
    Department of Neuroscience, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA
    J Cell Biochem 98:1054-61. 2006
  2. ncbi Stem cells and aging: expanding the possibilities
    M S Rao
    Laboratory of Neurosciences, National Institute on Aging Gerontology Research Center 4F02, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Mech Ageing Dev 122:713-34. 2001
  3. ncbi Disruption of neurogenesis by amyloid beta-peptide, and perturbed neural progenitor cell homeostasis, in models of Alzheimer's disease
    Norman J Haughey
    Laboratory of Neurosciences, National Institute on Aging Gerontology Research Center, Baltimore, Maryland 21224, USA
    J Neurochem 83:1509-24. 2002
  4. ncbi NTera2: a model system to study dopaminergic differentiation of human embryonic stem cells
    Catherine M Schwartz
    Gerontology Research Center, Stem Cell Biology Unit, Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
    Stem Cells Dev 14:517-34. 2005
  5. ncbi Massively parallel signature sequencing profiling of fetal human neural precursor cells
    Jingli Cai
    Stem Cell Biology Unit/Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, MD 21224, USA
    Stem Cells Dev 15:232-44. 2006
  6. ncbi Comparison of the gene expression profile of undifferentiated human embryonic stem cell lines and differentiating embryoid bodies
    Bhaskar Bhattacharya
    Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    BMC Dev Biol 5:22. 2005
  7. ncbi Development of antibodies to human embryonic stem cell antigens
    Jingli Cai
    Laboratory of Neurosciences, National Institute of Aging, Baltimore, MD 21224, USA
    BMC Dev Biol 5:26. 2005
  8. ncbi Conserved and divergent paths that regulate self-renewal in mouse and human embryonic stem cells
    Mahendra Rao
    Stem Cell Section, Laboratory of Neurosciences, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
    Dev Biol 275:269-86. 2004
  9. ncbi Stem sense: a proposal for the classification of stem cells
    Mahendra S Rao
    Stem Cell Section, Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
    Stem Cells Dev 13:452-5. 2004
  10. ncbi Microarray analysis of selected genes in neural stem and progenitor cells
    Yongquan Luo
    Laboratory of Neurosciences, Gerontology Research Center, National Institute on Aging, Room 4E02, 5600 Nathan Shock Drive, Baltimore, Maryland 21224, USA
    J Neurochem 83:1481-97. 2002

Collaborators

Detail Information

Publications98

  1. ncbi Are there morally acceptable alternatives to blastocyst derived ESC?
    Mahendra S Rao
    Department of Neuroscience, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA
    J Cell Biochem 98:1054-61. 2006
    ..All options need to be fully explored and decisions need to be made with scientific rigor and respect for each individual's moral compass...
  2. ncbi Stem cells and aging: expanding the possibilities
    M S Rao
    Laboratory of Neurosciences, National Institute on Aging Gerontology Research Center 4F02, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Mech Ageing Dev 122:713-34. 2001
    ....
  3. ncbi Disruption of neurogenesis by amyloid beta-peptide, and perturbed neural progenitor cell homeostasis, in models of Alzheimer's disease
    Norman J Haughey
    Laboratory of Neurosciences, National Institute on Aging Gerontology Research Center, Baltimore, Maryland 21224, USA
    J Neurochem 83:1509-24. 2002
    ..Adverse effects of Abeta on NPC may contribute to the depletion of neurons and cognitive impairment in AD...
  4. ncbi NTera2: a model system to study dopaminergic differentiation of human embryonic stem cells
    Catherine M Schwartz
    Gerontology Research Center, Stem Cell Biology Unit, Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
    Stem Cells Dev 14:517-34. 2005
    ..Therefore, NTera2 cell dopaminergic neuronal differentiation and PSA-NCAM enrichment provides a useful system for the future study of hESCs...
  5. ncbi Massively parallel signature sequencing profiling of fetal human neural precursor cells
    Jingli Cai
    Stem Cell Biology Unit/Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, MD 21224, USA
    Stem Cells Dev 15:232-44. 2006
    ..In addition, our results allow us to identify novel genes expressed in fNPCs and provide a detailed profile of fNPCs...
  6. ncbi Comparison of the gene expression profile of undifferentiated human embryonic stem cell lines and differentiating embryoid bodies
    Bhaskar Bhattacharya
    Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    BMC Dev Biol 5:22. 2005
    ....
  7. ncbi Development of antibodies to human embryonic stem cell antigens
    Jingli Cai
    Laboratory of Neurosciences, National Institute of Aging, Baltimore, MD 21224, USA
    BMC Dev Biol 5:26. 2005
    ..Embryonic stem cell research can be benefited from using antibodies specific to transcriptional factors/markers that contribute to the "stemness" phenotype or critical for cell lineage...
  8. ncbi Conserved and divergent paths that regulate self-renewal in mouse and human embryonic stem cells
    Mahendra Rao
    Stem Cell Section, Laboratory of Neurosciences, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
    Dev Biol 275:269-86. 2004
    ....
  9. ncbi Stem sense: a proposal for the classification of stem cells
    Mahendra S Rao
    Stem Cell Section, Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
    Stem Cells Dev 13:452-5. 2004
    ....
  10. ncbi Microarray analysis of selected genes in neural stem and progenitor cells
    Yongquan Luo
    Laboratory of Neurosciences, Gerontology Research Center, National Institute on Aging, Room 4E02, 5600 Nathan Shock Drive, Baltimore, Maryland 21224, USA
    J Neurochem 83:1481-97. 2002
    ....
  11. ncbi Sox2 expression defines a heterogeneous population of neurosphere-forming cells in the adult murine brain
    Christine Y Brazel
    Laboratory of Neurosciences, National Institute on Aging, Baltimore, MD 21224, USA
    Aging Cell 4:197-207. 2005
    ..Thus, we propose that the expression of Sox2 is a unifying characteristic of NSCs in the adult brain, but that not all NSCs maintain the ability to form all neural cell types in vivo...
  12. ncbi Neurogenesis in Talpha-1 tubulin transgenic mice during development and after injury
    Turhan Coksaygan
    Gerontology Research Center, Stem Cell Biology Unit Laboratory of Neuroscience, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Room 4E02, Baltimore, MD 21224, USA
    Exp Neurol 197:475-85. 2006
    ....
  13. ncbi Oligodendrocyte and astrocyte development in rodents: an in situ and immunohistological analysis during embryonic development
    Ying Liu
    Laboratory of Neurosciences, National Institute on Aging, Baltimore, Maryland 21224, USA
    Glia 40:25-43. 2002
    ..A novel dorsal domain of CD44 immunoreactivity that can be distinguished from the more ventral glial precursor domains can be detected as early as E13.5...
  14. ncbi Adult glial precursor proliferation in mutant SOD1G93A mice
    Tim Magnus
    Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland 21287, USA
    Glia 56:200-8. 2008
    ..These data suggest that adult glial progenitors from SOD1G93A mice differentially respond to inflammatory cytokines and contribute to the observed reactive astrocytosis observed in SOD1G93A mouse lumbar spinal cord...
  15. ncbi Nitric oxide acts in a positive feedback loop with BDNF to regulate neural progenitor cell proliferation and differentiation in the mammalian brain
    Aiwu Cheng
    Laboratory of Neurosciences, National Institute on Aging Gerontology Research Center, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Dev Biol 258:319-33. 2003
    ....
  16. ncbi Assessing self-renewal and differentiation in human embryonic stem cell lines
    Jingli Cai
    Laboratory of Neurosciences, National Institute on Aging, Department of Health and Human Services, Baltimore, Maryland, USA
    Stem Cells 24:516-30. 2006
    ..We show that qPCR, with as few as 12 selected genes, can reliably distinguish differentiated cells from undifferentiated hESC populations...
  17. ncbi Membrane properties of rat embryonic multipotent neural stem cells
    Jingli Cai
    Laboratory of Neurosciences, Gerontology Research Center, National Institute on Aging, Baltimore, Maryland 21224, USA
    J Neurochem 88:212-26. 2004
    ..Overall, our results show that fetal NSCs exhibit a unique signature that can be used to determine their location and assess their ability to respond to their environment...
  18. ncbi Monitoring early differentiation events in human embryonic stem cells by massively parallel signature sequencing and expressed sequence tag scan
    Takumi Miura
    Gerontology Research Center, Stem Cell Biology Unit/Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
    Stem Cells Dev 13:694-715. 2004
    ..The data generated can be used to monitor the state of hESC isolated by different laboratories using independent methods and maintained under differing culture conditions...
  19. ncbi Characterization of progenitor-cell-specific genes identified by subtractive suppression hybridization
    Jingli Cai
    Laboratoryof Neurosciences, National Institute on Aging, Baltimore, MD 21224, USA
    Dev Neurosci 26:131-47. 2004
    ..Overall, our results show that SSH can be used to identify lineage- and stage-specific markers and that extracellular matrix molecules likely play important roles in the migration and differentiation of GRPs...
  20. ncbi Immortalized neural stem cells differ from nonimmortalized cortical neurospheres and cerebellar granule cell progenitors
    Ruifa Mi
    Department of Neurology, Johns Hopkins University, Baltimore, MD 21287, USA
    Exp Neurol 194:301-19. 2005
    ..2 transplants but not with other cells. We suggest that, while C17.2 cells can illustrate many fundamental aspects of neural biology and are useful in their own right, their unique properties cannot be generalized...
  21. ncbi Human Schwann cells retain essential phenotype characteristics after immortalization
    Helmar C Lehmann
    Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Stem Cells Dev 21:423-31. 2012
    ..These cells are particularly useful for drug screening and studies aimed at disease mechanisms involving SCs...
  22. ncbi MPSS profiling of human embryonic stem cells
    Ralph Brandenberger
    National Institute on Aging GRC Laboratory of Neuroscience, 5600 Nathan Shock Drive Room 4E02 Baltimore, MD 21224, USA
    BMC Dev Biol 4:10. 2004
    ..Pooled human embryonic stem cells (hESC) cell lines were profiled to obtain a comprehensive list of genes common to undifferentiated human embryonic stem cells...
  23. ncbi Designing, testing, and validating a focused stem cell microarray for characterization of neural stem cells and progenitor cells
    Yongquan Luo
    Laboratory of Neurosciences, Gerontology Research Center, National Institute on Aging, Baltimore, Maryland, USA
    Stem Cells 21:575-87. 2003
    ..Thus, this focused neural stem array provides a convenient and useful tool for detection and assessment of NSCs and progenitor cells and can reliably distinguish them from other cell populations...
  24. ncbi Stem and precursor cells in the nervous system
    Mahendra Rao
    National Institute on Aging, Baltimore, Maryland 21224, USA
    J Neurotrauma 21:415-27. 2004
    ..The relative advantages and disadvantages of these cells for cell replacement therapy are discussed...
  25. ncbi SDF1alpha/CXCR4 signaling stimulates beta-catenin transcriptional activity in rat neural progenitors
    Yongquan Luo
    Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, Baltimore, MD, USA
    Neurosci Lett 398:291-5. 2006
    ..Our data suggest that SDF1alpha/CXCR4 signaling may interact with the Wnt/beta-catenin/TCF pathway to regulate the development of the central nervous system...
  26. ncbi Properties of a fetal multipotent neural stem cell (NEP cell)
    Jingli Cai
    Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
    Dev Biol 251:221-40. 2002
    ..Overall, our results show that a spectrum of markers used in combination can reliably distinguish multipotent NSCs from other precursor cells as well as differentiated cells present in the CNS...
  27. ncbi Designing, testing, and validating a microarray for stem cell characterization
    Yongquan Luo
    Stem Cell Biology Unit, Laboratory of Neurosciences, National Institute on Aging, Baltimore, MD, USA
    Methods Mol Biol 331:241-66. 2006
    ..Here we describe a protocol using this method to examine stem cells...
  28. ncbi Patterns of laminins and integrins in the embryonic ventricular zone of the CNS
    Justin D Lathia
    Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, 5600 Nathan Shock Drive, Baltimore, Maryland 21224, USA
    J Comp Neurol 505:630-43. 2007
    ....
  29. ncbi Disease progression after bone marrow transplantation in a model of multiple sclerosis is associated with chronic microglial and glial progenitor response
    Riccardo Cassiani-Ingoni
    Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA
    J Neuropathol Exp Neurol 66:637-49. 2007
    ..Our data indicate that reactivation of a local inflammatory process after BMT is sustained predominantly by endogenous microglia/macrophages...
  30. ncbi Olig genes are expressed in a heterogeneous population of precursor cells in the developing spinal cord
    Ying Liu
    Laboratory of Neurosciences, National Institute on Aging, Baltimore, Maryland 21224, USA
    Glia 45:67-74. 2004
    ....
  31. ncbi Comparisons between transcriptional regulation and RNA expression in human embryonic stem cell lines
    Audrey Player
    Advanced Technology Center, Microarray Facility, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Stem Cells Dev 15:315-23. 2006
    ..Collectively, these data provide insight into the relationship between gene expression and transcriptional regulation, resulting in a reliable list of genes associated with hESCs...
  32. ncbi Differences between human and mouse embryonic stem cells
    Irene Ginis
    Stem Cell Section, Laboratory of Neurosciences, National Institute on Aging, NIH, Baltimore, MD 21224, USA
    Dev Biol 269:360-80. 2004
    ..2) and to feeder-free subclones of H1, H7, and H9, indicating that the observed differences between human and mouse ES cells were species-specific rather than arising from differences in culture conditions...
  33. ncbi Focal transplantation-based astrocyte replacement is neuroprotective in a model of motor neuron disease
    Angelo C Lepore
    Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Nat Neurosci 11:1294-301. 2008
    ....
  34. ncbi Impaired SDF1/CXCR4 signaling in glial progenitors derived from SOD1(G93A) mice
    Yongquan Luo
    Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, Baltimore, Maryland, USA
    J Neurosci Res 85:2422-32. 2007
    ..These data indicate that the abnormalities in SOD1(G93A) glial progenitor expression of CXCR4 and its mediated signaling and function occur during spinal cord development and highlight nonneuronal (glial) abnormalities in this ALS model...
  35. ncbi A focused microarray to assess dopaminergic and glial cell differentiation from fetal tissue or embryonic stem cells
    Yongquan Luo
    Gerontology Research Center, Stem Cell Biology Unit, Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Department of Health and Human Services, Baltimore, Maryland, USA
    Stem Cells 24:865-75. 2006
    ..We suggest that this focused array will serve as a useful complement to other large-scale arrays in routine assessment of cell properties prior to their therapeutic use...
  36. ncbi Glutamate transporter expression and function in human glial progenitors
    Nicholas J Maragakis
    Department of Neurology and Neuroscience, Johns Hopkins University, Meyer 6-109, 600 N. Wolfe Street, Baltimore, MD 21287-5953, USA
    Glia 45:133-43. 2004
    ....
  37. ncbi Human embryonic stem cells have enhanced repair of multiple forms of DNA damage
    Scott Maynard
    Laboratory of Molecular Gerontology, National Institute on Aging, NIH, Box 1, 5600 Nathan Shock Drive, Baltimore, Maryland 21224 6825, USA
    Stem Cells 26:2266-74. 2008
    ..These data suggest that genomic maintenance pathways are enhanced in human embryonic stem cells, relative to differentiated human cells...
  38. ncbi Gap junctional communication is required to maintain mouse cortical neural progenitor cells in a proliferative state
    Aiwu Cheng
    Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, Baltimore, MD 21224, USA
    Dev Biol 272:203-16. 2004
    ..In addition to promoting their proliferation, gap junctions are required for the survival of NPCs. Gap junctional communication is therefore both necessary and sufficient to maintain NPCs in a self-renewing state...
  39. ncbi Truncated tyrosine kinase B brain-derived neurotrophic factor receptor directs cortical neural stem cells to a glial cell fate by a novel signaling mechanism
    Aiwu Cheng
    Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, Baltimore, Maryland 21224, USA
    J Neurochem 100:1515-30. 2007
    ..Our findings suggest a mechanism by which a single factor (BDNF) regulates the production of the two major cell types in the mammalian cerebral cortex...
  40. ncbi Neural stem cell therapy in the aging brain: pitfalls and possibilities
    Tobi L Limke
    Laboratory of Neurosciences, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
    J Hematother Stem Cell Res 12:615-23. 2003
    ..In this review, we focus on recent progresses made toward understand the biology of neural stem cells in the aging brain, as well as progress toward using stem cells to replace cells lost during disease...
  41. ncbi Cytoplasmic translocation of Olig2 in adult glial progenitors marks the generation of reactive astrocytes following autoimmune inflammation
    Riccardo Cassiani-Ingoni
    Cellular Immunology Section, Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    Exp Neurol 201:349-58. 2006
    ..Together, these data ascribe a pivotal role to Olig2+ glial precursor cells in the adult CNS, linking autoimmune inflammation and glial scar formation...
  42. ncbi Conservation and variation of gene regulation in embryonic stem cells assessed by comparative genomics
    Ming Zhan
    Bioinformatics Unit, Branch of Research Resources, National Institute on Aging, NIH, Baltimore, MD, 21224, USA
    Cell Biochem Biophys 43:379-405. 2005
    ..These results provide a framework for examining the current reported differences between rodent and human ES cells and define targets for future perturbation studies...
  43. ncbi Gene expression profile of neuronal progenitor cells derived from hESCs: activation of chromosome 11p15.5 and comparison to human dopaminergic neurons
    William J Freed
    Cellular Neurobiology Research Branch, Intramural Research Program IRP, National Institute on Drug Abuse, National Institutes of Health NIH, Baltimore, Maryland, USA
    PLoS ONE 3:e1422. 2008
    ..We initiated differentiation of human embryonic stem cells (hESCs) into dopamine neurons, obtained a purified population of neuronal precursor cells by cell sorting, and determined patterns of gene transcription...
  44. ncbi Genome wide profiling of human embryonic stem cells (hESCs), their derivatives and embryonal carcinoma cells to develop base profiles of U.S. Federal government approved hESC lines
    Ying Liu
    Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland 21224, USA
    BMC Dev Biol 6:20. 2006
    ....
  45. ncbi Transcriptome coexpression map of human embryonic stem cells
    Huai Li
    Bioinformatics Unit, Branch of Research Resources, National Institute on Aging, NIH, Baltimore, MD 21224, USA
    BMC Genomics 7:103. 2006
    ..However, no systematic analysis has yet addressed whether gene expression in human ES cells may be regulated in chromosomal domains, and no chromosomal domains of coexpression have been identified...
  46. ncbi Cross-species transcriptional profiles establish a functional portrait of embryonic stem cells
    Yu Sun
    Bioinformatics Unit, Research Resources Branch, National Institute on Aging, National Institutes of Health, 333 Cassall Drive, Baltimore, MD 21224, USA
    Genomics 89:22-35. 2007
    ..The data and analyses resulting from this study provide a framework for new hypotheses and research directions and a public resource for functional genomics of ESCs...
  47. ncbi Evidence that nucleocytoplasmic Olig2 translocation mediates brain-injury-induced differentiation of glial precursors to astrocytes
    Tim Magnus
    Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Neurosci Res 85:2126-37. 2007
    ..In contrast, neural stem cells and neuronal progenitor cells appear to play only a minor role in the injured adult CNS...
  48. ncbi Revisiting the astrocyte-oligodendrocyte relationship in the adult CNS
    Jessica Carmen
    Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, NIH, Baltimore, MD 21224, USA
    Prog Neurobiol 82:151-62. 2007
    ..Ideally, better understanding the origin of new glial cells in the injured CNS will facilitate the development of therapeutics targeted to alter the glial response in a beneficial way...
  49. ncbi Aging and neuronal replacement
    Christine Y Brazel
    Laboratory of Neurosciences, National Institute on Aging, 333 Cassell Dr, Triad 406A, Baltimore, MD 21224, USA
    Ageing Res Rev 3:465-83. 2004
    ..Pre-differentiation of neural stem cells into immature neurons prior to transplantation can also aid in functional recovery following injury or disease...
  50. ncbi Dopaminergic differentiation of human embryonic stem cells
    Xianmin Zeng
    Cellular Neurobiology Research Branch, National Institute on Drug Abuse, Baltimore, Maryland 21224, USA
    Stem Cells 22:925-40. 2004
    ..Therefore, hESCs can be used to generate human dopaminergic cells that exhibit biochemical and functional properties consistent with the expected properties of mature dopaminergic neurons...
  51. ncbi Neural stem cells in aging and disease
    T L Limke
    National Institute on Aging, Baltimore, MD 21224, USA
    J Cell Mol Med 6:475-96. 2002
    ..This review focuses on recent advances in the field of stem cell biology of the central nervous system, with an emphasis on cellular and molecular approaches to replacing cells lost in neurodegenerative disorders...
  52. ncbi Transdifferentiation--fact or artifact
    Ying Liu
    Laboratory of Neurosciences, National Institute on Aging, 5600 Nathan Shock Drive, Baltimore, Maryland 21224, USA
    J Cell Biochem 88:29-40. 2003
    ..We predict, however, that transdifferentiation will not be reliable or reproducible and will probably require complex manipulations...
  53. ncbi Glial progenitors in the CNS and possible lineage relationships among them
    Ying Liu
    Laboratory of Neurosciences, National Institute on Aging, Baltimore, MD 21224, USA
    Biol Cell 96:279-90. 2004
    ..We believe that the development of consensus sets of markers and an improvement in our ability to define stages of glial maturation will lead to a clearer appreciation of the importance of glia in the etiopathology of disease...
  54. ncbi Properties of pluripotent human embryonic stem cells BG01 and BG02
    Xianmin Zeng
    Development and Plasticity Section, Cellular Neurobiology Research Branch, National Institute on Drug Abuse, Department of Health and Human Services, Baltimore, Maryland 21224, USA
    Stem Cells 22:292-312. 2004
    ..These data provide a detailed characterization and an initial gene expression profile for the BG01 and BG02 human ES cell lines...
  55. ncbi Distinguishing features of progenitor cells in the late embryonic and adult hippocampus
    Tobi L Limke
    National Institute on Aging, Laboratory of Neurosciences, Gerontology Research Center, Baltimore, MD 21224, USA
    Dev Neurosci 25:257-72. 2003
    ....
  56. ncbi CD44 expression identifies astrocyte-restricted precursor cells
    Ying Liu
    Laboratory of Neurosciences, Gerontology Research Center, National Institute on Aging, Baltimore, MD 21224, USA
    Dev Biol 276:31-46. 2004
    ..Misexpression of CD44 in culture inhibits oligodendrocytes and arrests cells at the precursor state. In summary, our data provide strong evidence for the existence of a CD44+ ARP in the developing nervous system...
  57. ncbi Glial restricted precursors protect against chronic glutamate neurotoxicity of motor neurons in vitro
    Nicholas J Maragakis
    Department of Neurology and Neuroscience, Johns Hopkins University, Baltimore, Maryland 21287, USA
    Glia 50:145-59. 2005
    ..The seeding of G3s resulted in a reduction of motor neuron cell death. Hence, we believe that these cells may potentially play a role in cell-based neuroprotection from glutamate excitotoxicity...
  58. ncbi BG01V: a variant human embryonic stem cell line which exhibits rapid growth after passaging and reliable dopaminergic differentiation
    Xianmin Zeng
    Cellular Neurobiology Branch, National Institute on Drug Abuse and NIH, DHHS, Baltimore, MD 21224, USA
    Restor Neurol Neurosci 22:421-8. 2004
    ..To explore a karyotypically abnormal variant human embryonic stem cell (hESC) line, BG01V, as a potential model for studies of dopaminergic neuronal differentiation...
  59. ncbi In search of "stemness"
    Jingli Cai
    Gerontology Research Center, Stem Cell Biology Unit/Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
    Exp Hematol 32:585-98. 2004
    ..While we predict many similarities, we also predict that differences will exist between stem cell populations and that when transdifferentiation is considered genes expected to be both similar and different need to be examined...
  60. ncbi Stage-dependent Olig2 expression in motor neurons and oligodendrocytes differentiated from embryonic stem cells
    Soojung Shin
    Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, Baltimore, MD 21224, USA
    Stem Cells Dev 16:131-41. 2007
    ....
  61. ncbi A central nervous system specific mouse model for thanatophoric dysplasia type II
    Ti Lin
    Laboratory of Genetics, National Institute on Aging, Baltimore Maryland, 21224 6814, USA
    Hum Mol Genet 12:2863-71. 2003
    ..Overall, these data indicate that the tissue-specific mouse model is an excellent system for studying the role of Fgfr3 in the developing CNS...
  62. ncbi Mechanisms controlling embryonic stem cell self-renewal and differentiation
    Yu Sun
    Bioinformatics Unit, Research Resources Branch, National Institute on Aging, NIH, Baltimore, MD 21224, USA
    Crit Rev Eukaryot Gene Expr 16:211-31. 2006
    ..Here, we review recent findings on mechanisms controlling ES cell development. By integrating data from different sources, we present a global picture of how ES cells reach the decision of self-renewal or differentiation...
  63. ncbi Large-scale analysis of neural stem cells and progenitor cells
    Soojung Shin
    Gerontology Research Center, Stem Cell Biology Unit/Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
    Neurodegener Dis 3:106-11. 2006
    ..We suggest that while much has been learned, additional information remains to be gleaned by meta-analysis of existing data...
  64. ncbi Stem cell and precursor cell therapy
    Jingli Cai
    Laboratory of Neurosciences, National Institute on Aging, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Neuromolecular Med 2:233-49. 2002
    ....
  65. ncbi Genomic alterations in cultured human embryonic stem cells
    Anirban Maitra
    McKusick-Nathans Institute of Genetic Medicine, Department of Pathology, The Sol Goldman Pancreatic Cancer Research Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Nat Genet 37:1099-103. 2005
    ....
  66. ncbi Hyaluronan accumulates in demyelinated lesions and inhibits oligodendrocyte progenitor maturation
    Stephen A Back
    Department of Pediatrics, School of Medicine, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239, USA
    Nat Med 11:966-72. 2005
    ..HMW hyaluronan may therefore contribute substantially to remyelination failure by preventing the maturation of OPCs that are recruited to demyelinating lesions...
  67. ncbi Evolutionarily conserved transcriptional co-expression guiding embryonic stem cell differentiation
    Yu Sun
    Bioinformatics Unit, Research Resources Branch, National Institute on Aging, NIH, Baltimore, MD, USA
    PLoS ONE 3:e3406. 2008
    ..Cross-species examination of transcriptional co-expression allows elucidation of fundamental and species-specific mechanisms regulating ESC self-renewal or differentiation...
  68. ncbi Cellular lifespan and senescence signaling in embryonic stem cells
    Takumi Miura
    Stem Cell Biology Unit, Laboratory of Neurosciences, National Institute on Aging, Gerontology Research Center, Baltimore, MD 21224, USA
    Aging Cell 3:333-43. 2004
    ..In this review, we summarize recent findings related to these pathways in embryonic stem (ES) cells and suggest that ES cells are immortal because these pathways are tightly regulated...
  69. ncbi An in vitro model of human dopaminergic neurons derived from embryonic stem cells: MPP+ toxicity and GDNF neuroprotection
    Xianmin Zeng
    Intramural Research Program IRP, Cellular Neurobiology Research Branch, Department of Health and Human Services DHHS, National Institute on Drug Abuse NIDA, National Institutes of Health NIH, Baltimore, MD, USA
    Neuropsychopharmacology 31:2708-15. 2006
    ....
  70. ncbi Toward cell replacement therapy: promises and caveats
    Irene Ginis
    Gerontology Research Center, Stem Cell Biology Unit/Laboratory of Neuroscience, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Room 4E02, Baltimore, MD 21224, USA
    Exp Neurol 184:61-77. 2003
    ....
  71. ncbi Functional SDF1 alpha/CXCR4 signaling in the developing spinal cord
    Yongquan Luo
    Laboratory of Neurosciences, Gerontology Research Center, National Institute on Aging, Baltimore, Maryland 21224, USA
    J Neurochem 93:452-62. 2005
    ..Thus, our data suggest that SDF1alpha may function through a CXCR4/ERK/Ets-linked signalling pathway in spinal cord neural development to modulate migration of progenitor cells...
  72. ncbi The microenvironment of the embryonic neural stem cell: lessons from adult niches?
    Justin D Lathia
    Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, Baltimore, Maryland, USA
    Dev Dyn 236:3267-82. 2007
    ..Finally, we will ask how these lessons from the niche inform our understanding of neurodevelopmental diseases and cancers of the CNS...
  73. ncbi Stem cell myths
    Tim Magnus
    Stem Cell Section, Laboratory of Neurosciences, National Institute on Aging, NIH, 333 Cassell Drive, Room 406A, Baltimore, MD 21224, USA
    Philos Trans R Soc Lond B Biol Sci 363:9-22. 2008
    ..Data need to be collected and reported for failed as well as successful experiments and a rigorous scientific approach taken to evaluate the undeniable promise of stem cell biology...
  74. ncbi Identifying and tracking neural stem cells
    Jingli Cai
    Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84132, USA
    Blood Cells Mol Dis 31:18-27. 2003
    ..It will therefore be necessary to develop invasive and non-invasive strategies to follow transplanted cells and develop useful quantifiable readouts. Some potential strategies are described and current results are discussed...
  75. ncbi Regulatory networks define phenotypic classes of human stem cell lines
    Franz Josef Muller
    Center for Regenerative Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 455:401-5. 2008
    ..Our results offer a new strategy for classifying stem cells and support the idea that pluripotency and self-renewal are under tight control by specific molecular networks...
  76. ncbi Grafted lineage-restricted precursors differentiate exclusively into neurons in the adult spinal cord
    Steve S W Han
    Department of Neurobiology and Anatomy, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, Pennsylvania 19129, USA [corrected
    Exp Neurol 177:360-75. 2002
    ..We conclude that the adult nonneurogenic CNS environment does not support the transition of multipotential NSCs to the neuronal commitment stage, but does allow the survival, maturation, and integration of NRP cells...
  77. ncbi Isolation of a glial-restricted tripotential cell line from embryonic spinal cord cultures
    Yuan Yuan Wu
    Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah, USA
    Glia 38:65-79. 2002
    ....
  78. ncbi Isolation of stem and precursor cells from fetal tissue
    Yuan Y Wu
    Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, UT, USA
    Methods Mol Biol 198:29-40. 2002
  79. ncbi Increased dentate neurogenesis after grafting of glial restricted progenitors or neural stem cells in the aging hippocampus
    Bharathi Hattiangady
    Division of Neurosurgery, Duke University Medical Center, Durham, NC 27710, USA
    Stem Cells 25:2104-17. 2007
    ..Grafting of GRPs or NSCs therefore provides an attractive approach for improving neurogenesis in the aging hippocampus. Disclosure of potential conflicts of interest is found at the end of this article...
  80. ncbi Stable expression of the alkaline phosphatase marker gene by neural cells in culture and after transplantation into the CNS using cells derived from a transgenic rat
    Tahmina Mujtaba
    Department of Neurobiology and Anatomy, University of Utah School of Medicine, 50 North Medical Drive, Salt Lake City, Utah 84132, USA
    Exp Neurol 174:48-57. 2002
    ..We submit that cells isolated from this transgenic rat will be valuable for studies of neural development and regeneration...
  81. ncbi PDGF, TGF-beta, and FGF signaling is important for differentiation and growth of mesenchymal stem cells (MSCs): transcriptional profiling can identify markers and signaling pathways important in differentiation of MSCs into adipogenic, chondrogenic, and o
    Felicia Ng
    Genome and Gene Expression Analysis Group, Bioinformatics Institute, Agency for Science Technology and Research A STAR, Singapore
    Blood 112:295-307. 2008
    ..Thus, this study illustrates it is possible to predict signaling pathways active in cellular differentiation and growth using microarray data and experimentally verify these predictions...
  82. ncbi Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research
    Richard Josephson
    GlobalStem, Inc, 6 Taft Court, Rockville, Maryland 20850, USA
    Stem Cells 25:437-46. 2007
    ..On the basis of global gene and microRNA expression, 2102Ep is somewhat less similar to hESC than the alternatives; however, 2102Ep expresses more hESC-associated microRNAs than NTERA-2 does, and fewer markers of differentiated fates...
  83. ncbi Endogenous Nkx2.2+/Olig2+ oligodendrocyte precursor cells fail to remyelinate the demyelinated adult rat spinal cord in the absence of astrocytes
    Jason F Talbott
    The MD PhD Program, University of Louisville, Louisville, KY 40292, USA
    Exp Neurol 192:11-24. 2005
    ..In vivo electrophysiology demonstrated a limited and unsustained functional recovery concurrent with endogenous remyelination following EB-induced lesions...
  84. ncbi Karyotypic stability, genotyping, differentiation, feeder-free maintenance, and gene expression sampling in three human embryonic stem cell lines derived prior to August 9, 2001
    Sandii N Brimble
    BresaGen, Inc, Athens, GA 30605, USA
    Stem Cells Dev 13:585-97. 2004
    ..BG01, BG02, and BG03 hESCs are therefore independent, undifferentiated, and pluripotent lines that can be maintained without accumulation of karyotypic abnormalities...
  85. ncbi Brg1 is required for murine neural stem cell maintenance and gliogenesis
    Steven Matsumoto
    Integrative Biosciences Department, School of Dentistry, Oregon Health and Science University, Portland, 97239, USA
    Dev Biol 289:372-83. 2006
    ....
  86. ncbi An efficient method for the derivation of mouse embryonic stem cells
    Vítezlav Bryja
    Laboratory of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet S 171 77, Stockholm, Sweden
    Stem Cells 24:844-9. 2006
    ..In summary, we provide a simplified and improved protocol to derive mESCs that can be useful for laboratories aiming to isolate transgenic mESCs for the first time...
  87. ncbi Neural precursor cells can be delivered into the injured cervical spinal cord by intrathecal injection at the lumbar cord
    Angelo C Lepore
    Department of Neurobiology and Anatomy, 2900 Queen Lane, Drexel University College of Medicine, Philadelphia, PA 19129, USA
    Brain Res 1045:206-16. 2005
    ..These results demonstrate the potential of delivering lineage-restricted NPCs using the minimally invasive lumbar puncture method for the treatment of spinal cord injury...
  88. ncbi Characterization and in vitro differentiation potential of a new human embryonic stem cell line, ReliCellhES1
    Arundhati Mandal
    Embryonic Stem Cell Group, Reliance Life Sciences Pvt. Ltd, Dhirubhai Ambani Life Sciences Center, R-282, TTC Industrial Area of MIDC Thane-Belapur Road, Rabale, Navi Mumbai 400 701, India
    Differentiation 74:81-90. 2006
    ..To our knowledge, this is the first report of a well-characterized hES cell line from the Indian subcontinent...
  89. ncbi CD44 overexpression by oligodendrocytes: a novel mouse model of inflammation-independent demyelination and dysmyelination
    Therese M F Tuohy
    Division of Neuroscience, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton 97006, USA
    Glia 47:335-45. 2004
    ..These findings suggest that CNPase-CD44 mice are a novel model for noninflammatory progressive demyelinating disease and support a potential role for CD44 proteins expressed by glial cells in promoting demyelination...
  90. ncbi Hes1 but not Hes5 regulates an astrocyte versus oligodendrocyte fate choice in glial restricted precursors
    Yuanyuan Wu
    Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah, USA
    Dev Dyn 226:675-89. 2003
    ..Overexpression of Notch1 has an effect on gliogenesis similar to that of Hes1 and the mRNA levels of Hes1 are up-regulated in cells overexpressing Notch1, suggesting that Notch1 could be an upstream activator of Hes1...
  91. ncbi Derivation and characterization of neuronal precursors and dopaminergic neurons from human embryonic stem cells in vitro
    Melissa Carpenter
    The John P. Robarts Research Institute, Stem Cell Biology and Regenerative Medicine, London, Ontario, Canada
    Methods Mol Biol 331:153-67. 2006
    ....
  92. ncbi Long-term culture of human embryonic stem cells in feeder-free conditions
    Elen S Rosler
    Geron Corporation, Menlo Park, California, USA
    Dev Dyn 229:259-74. 2004
    ....
  93. ncbi Massively parallel signature sequencing
    Daixing Zhou
    Lynx Therapeutics Inc, Hayward, CA, USA
    Methods Mol Biol 331:285-311. 2006
    ..This chapter describes the technology and its application in establishing stem cell transcriptome databases...
  94. ncbi Properties of four human embryonic stem cell lines maintained in a feeder-free culture system
    Melissa K Carpenter
    Geron Corporation, Menlo Park, California, USA
    Dev Dyn 229:243-58. 2004
    ..These data provide the first detailed comparison of different hES cell lines and demonstrate remarkable similarities among lines maintained in identical culture conditions...
  95. ncbi The therapeutic potential of embryonic stem cells: A focus on stem cell stability
    Xianmin Zeng
    Buck Institute for Age Research, 8001 Redwood Boulevard, Novato, CA 94945, USA
    Curr Opin Mol Ther 8:338-44. 2006
    ..Recent findings suggesting that the level of cell stability is not homogeneous throughout all stem cells are also discussed...
  96. ncbi Whole genome analysis of human neural stem cells derived from embryonic stem cells and stem and progenitor cells isolated from fetal tissue
    Soojung Shin
    Stem Cells and Regenerative Medicine, Invitrogen, Carlsbad, California, USA
    Stem Cells 25:1298-306. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  97. ncbi Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation
    Ralph Brandenberger
    Geron Corporation, Menlo Park, California 94025, USA
    Nat Biotechnol 22:707-16. 2004
    ..The genomic data presented here may facilitate the derivation of clinically useful cell types from hES cells...
  98. ncbi Monitoring differentiation of human embryonic stem cells using real-time PCR
    Karin Noaksson
    Cellartis AB, Arvid Wallgrens Backe 20, , Sweden
    Stem Cells 23:1460-7. 2005
    ..The combination of QPCR with hESC-based technologies opens novel avenues for high-throughput analysis of hESCs in, for example, pharmacological and cytotoxicity screening...