Paul A Randazzo

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Differences between AGAP1, ASAP1 and Arf GAP1 in substrate recognition: interaction with the N-terminus of Arf1
    Hye Young Yoon
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Building 37, Room 4118, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Signal 16:1033-44. 2004
  2. pmc Kinetic analysis of Arf GAP1 indicates a regulatory role for coatomer
    RuiBai Luo
    Laboratory of Cellular and Molecular Biology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 283:21965-77. 2008
  3. pmc Consensus nomenclature for the human ArfGAP domain-containing proteins
    Richard A Kahn
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Cell Biol 182:1039-44. 2008
  4. pmc The Arf GTPase-activating protein ASAP1 regulates the actin cytoskeleton
    P A Randazzo
    Division of Basic Sciences, National Cancer Institute, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 97:4011-6. 2000
  5. ncbi request reprint Arf GAPs: multifunctional proteins that regulate membrane traffic and actin remodelling
    Paul A Randazzo
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Building 37 Room 4118, Bethesda, MD 20892, USA
    Cell Signal 16:401-13. 2004
  6. ncbi request reprint Functional interaction of ADP-ribosylation factor 1 with phosphatidylinositol 4,5-bisphosphate
    P A Randazzo
    Laboratory of Biological Chemistry, Division of Basic Sciences, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 272:7688-92. 1997
  7. ncbi request reprint Molecular aspects of the cellular activities of ADP-ribosylation factors
    P A Randazzo
    Laboratory of Cellular Oncology, Division of Basic Sciences, National Cancer Institute, Bethesda, MD, USA
    Sci STKE 2000:re1. 2000
  8. ncbi request reprint Arf GAPs as regulators of the actin cytoskeleton
    Paul A Randazzo
    Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Biol Cell 99:583-600. 2007
  9. pmc A PH domain in the Arf GTPase-activating protein (GAP) ARAP1 binds phosphatidylinositol 3,4,5-trisphosphate and regulates Arf GAP activity independently of recruitment to the plasma membranes
    Fanny Campa
    Laboratory of Cellular and Molecular Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 284:28069-83. 2009
  10. pmc ASAP3 is a focal adhesion-associated Arf GAP that functions in cell migration and invasion
    Vi Luan Ha
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 283:14915-26. 2008

Collaborators

Detail Information

Publications62

  1. ncbi request reprint Differences between AGAP1, ASAP1 and Arf GAP1 in substrate recognition: interaction with the N-terminus of Arf1
    Hye Young Yoon
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Building 37, Room 4118, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Signal 16:1033-44. 2004
    ..Taken together, our results indicate that (i) Arf GAPs interact with amino acids 2-17 of Arf1 and (ii) each subgroup of Arf GAPs has a unique interface with Arf1...
  2. pmc Kinetic analysis of Arf GAP1 indicates a regulatory role for coatomer
    RuiBai Luo
    Laboratory of Cellular and Molecular Biology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 283:21965-77. 2008
    ..We conclude that coatomer is an allosteric regulator of Arf GAP1. The relevance of the results to the two models of Arf GAP1-mediated regulation of Arf1 is discussed...
  3. pmc Consensus nomenclature for the human ArfGAP domain-containing proteins
    Richard A Kahn
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Cell Biol 182:1039-44. 2008
    ..This article describes the resulting consensus nomenclature and provides a brief description of each of the 10 subfamilies of 31 human genes encoding proteins containing the ArfGAP domain...
  4. pmc The Arf GTPase-activating protein ASAP1 regulates the actin cytoskeleton
    P A Randazzo
    Division of Basic Sciences, National Cancer Institute, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 97:4011-6. 2000
    ..These data support a role for an Arf GTPase-activating protein, ASAP1, as a regulator of cytoskeletal remodeling and raise the possibility that the Arf pathway is a target for PDGF signaling...
  5. ncbi request reprint Arf GAPs: multifunctional proteins that regulate membrane traffic and actin remodelling
    Paul A Randazzo
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Building 37 Room 4118, Bethesda, MD 20892, USA
    Cell Signal 16:401-13. 2004
    ..The results discussed illustrate roles for both Arf-dependent and -independent activities in the regulation of cellular architecture...
  6. ncbi request reprint Functional interaction of ADP-ribosylation factor 1 with phosphatidylinositol 4,5-bisphosphate
    P A Randazzo
    Laboratory of Biological Chemistry, Division of Basic Sciences, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 272:7688-92. 1997
    ..These data support the hypothesis that PIP2 binding to Arf1 promotes interaction with Arf GAP. The implications of lipid-directed protein-protein interactions for membrane traffic are discussed...
  7. ncbi request reprint Molecular aspects of the cellular activities of ADP-ribosylation factors
    P A Randazzo
    Laboratory of Cellular Oncology, Division of Basic Sciences, National Cancer Institute, Bethesda, MD, USA
    Sci STKE 2000:re1. 2000
    ..Understanding Arf's role in complex cellular functions such as protein secretion or cell movement will involve a description of the temporal and spatial coordination of these multiple Arf-dependent events...
  8. ncbi request reprint Arf GAPs as regulators of the actin cytoskeleton
    Paul A Randazzo
    Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Biol Cell 99:583-600. 2007
    ..With multiple functional elements, the Arf GAPs could integrate signals and biochemical activities that result in co-ordinated changes in actin and membranes necessary for a wide range of cellular functions...
  9. pmc A PH domain in the Arf GTPase-activating protein (GAP) ARAP1 binds phosphatidylinositol 3,4,5-trisphosphate and regulates Arf GAP activity independently of recruitment to the plasma membranes
    Fanny Campa
    Laboratory of Cellular and Molecular Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 284:28069-83. 2009
    ..In our model, ARAP1 is recruited to membranes independently of PtdIns(3,4,5)P(3), the subsequent production of which triggers enzymatic activity...
  10. pmc ASAP3 is a focal adhesion-associated Arf GAP that functions in cell migration and invasion
    Vi Luan Ha
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 283:14915-26. 2008
    ..In comparing ASAP1 and ASAP3, we also found that invadopodia are dispensable for the invasive behavior of cells derived from a mammary carcinoma...
  11. ncbi request reprint Regulation of ASAP1 by phospholipids is dependent on the interface between the PH and Arf GAP domains
    Magnus M Che
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, United States
    Cell Signal 17:1276-88. 2005
    ....
  12. pmc Src-dependent phosphorylation of ASAP1 regulates podosomes
    Sanita Bharti
    Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Mol Cell Biol 27:8271-83. 2007
    ....
  13. ncbi request reprint ARAP2 effects on the actin cytoskeleton are dependent on Arf6-specific GTPase-activating-protein activity and binding to RhoA-GTP
    Hye Young Yoon
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Department of Health and Human Services, Building 37, Bethesda, MD 20892, USA
    J Cell Sci 119:4650-66. 2006
    ..We conclude that ARAP2 is an Arf6GAP that functions downstream of RhoA to regulate focal adhesion dynamics...
  14. ncbi request reprint Arf GAPs and their interacting proteins
    Hiroki Inoue
    Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Traffic 8:1465-75. 2007
    ..Here we describe the Arf GAP family and summarize the currently identified protein interactors in the context of known Arf GAP functions...
  15. pmc Arf GTPase-activating protein ASAP1 interacts with Rab11 effector FIP3 and regulates pericentrosomal localization of transferrin receptor-positive recycling endosome
    Hiroki Inoue
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 19:4224-37. 2008
    ..The depletion also altered the trafficking of endocytosed transferrin. These results support the conclusion that ASAP1, like FIP3, functions as a component of the endocytic recycling compartment...
  16. pmc Phosphatidylinositol-4-phosphate 5-kinase and GEP100/Brag2 protein mediate antiangiogenic signaling by semaphorin 3E-plexin-D1 through Arf6 protein
    Atsuko Sakurai
    Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 286:34335-45. 2011
    ....
  17. pmc ARAP2 Signals through Arf6 and Rac1 to Control Focal Adhesion Morphology
    Pei wen Chen
    From the Laboratory of Cellular and Molecular Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 288:5849-60. 2013
    ..We conclude that changes in Arf6 and Rac1 activities are necessary but not sufficient for ARAP2 to promote the growth of FAs and we speculate that ARAP2 has additional functions that are effector in nature to promote or stabilize FAs...
  18. pmc Modifications to the C-terminus of Arf1 alter cell functions and protein interactions
    Xiaoying Jian
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bldg 37 Room 2042, Bethesda, MD 20892, USA
    Traffic 11:732-42. 2010
    ....
  19. ncbi request reprint Mutational analysis of the Arf1*GTP/Arf GAP interface reveals an Arf1 mutant that selectively affects the Arf GAP ASAP1
    RuiBai Luo
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Curr Biol 15:2164-9. 2005
    ..Based on our results, we conclude that the contribution of specific residues within switch 1 of Arf to binding and achieving a transition state toward GTP hydrolysis differs among Arf GAPs...
  20. ncbi request reprint A BAR domain in the N terminus of the Arf GAP ASAP1 affects membrane structure and trafficking of epidermal growth factor receptor
    Zhongzhen Nie
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    Curr Biol 16:130-9. 2006
    ..We have examined the role of a putative BAR domain in the function of the Arf GAP ASAP1...
  21. pmc Kinetic analysis of GTP hydrolysis catalysed by the Arf1-GTP-ASAP1 complex
    RuiBai Luo
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Biochem J 402:439-47. 2007
    ..The ramifications for the putative effector function of ASAP1 are discussed...
  22. ncbi request reprint The Arf GAPs AGAP1 and AGAP2 distinguish between the adaptor protein complexes AP-1 and AP-3
    Zhongzhen Nie
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Sci 118:3555-66. 2005
    ..Based on these results, we concluded that the closely related Arf GAPs, AGAP1 and AGAP2, distinguish between these related heterotetrameric adaptor protein complexes to specifically regulate AP-3 endosomes and AP-1 recycling endosomes...
  23. pmc ARAP1 association with CIN85 affects epidermal growth factor receptor endocytic trafficking
    Hye Young Yoon
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892, U S A
    Biol Cell 103:171-84. 2011
    ..To further understand the function of ARAP1, we sought to identify proteins that interact with ARAP1...
  24. pmc ARAP1 regulates endocytosis of EGFR
    Hye Young Yoon
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA
    Traffic 9:2236-52. 2008
    ..Based on these findings, we propose that ARAP1 regulates the endocytic traffic of EGFR and, consequently, the rate of EGFR signal attenuation...
  25. pmc Arf GAP2 is positively regulated by coatomer and cargo
    RuiBai Luo
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA
    Cell Signal 21:1169-79. 2009
    ..Based on the common regulatory features of Arf GAP1 and 2, we propose a mechanism for cargo selection in which GTP hydrolysis triggered by cargo binding to the coat protein is coupled to coat polymerization...
  26. pmc Autoinhibition of Arf GTPase-activating protein activity by the BAR domain in ASAP1
    Xiaoying Jian
    Laboratory of Cellular and Molecular Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 284:1652-63. 2009
    ..Analysis of single turnover kinetics revealed a transition state intermediate that was affected by the BAR domain. We conclude that BAR domains can affect enzymatic activity through intraprotein interactions...
  27. pmc Dynamic interaction between Arf GAP and PH domains of ASAP1 in the regulation of GAP activity
    RuiBai Luo
    Laboratory of Cellular and Molecular Biology, National Institutes of Health, Bethesda, MD 20892, United Sates
    Cell Signal 20:1968-77. 2008
    ..We propose that the PH domain contributes to Arf GAP activity by either binding to or positioning Arf1 GTP that is simultaneously bound to the Arf GAP domain...
  28. doi request reprint Contribution of AZAP-Type Arf GAPs to cancer cell migration and invasion
    Vi Luan Ha
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, Maryland, USA
    Adv Cancer Res 101:1-28. 2008
    ..We also discuss the enzymology of the Arf GAPs related to possible targeted inhibition of specific subtypes of Arf GAPs...
  29. ncbi request reprint AGAP1, an endosome-associated, phosphoinositide-dependent ADP-ribosylation factor GTPase-activating protein that affects actin cytoskeleton
    Zhongzhen Nie
    Laboratories of Cellular Oncology and Biochemistry, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:48965-75. 2002
    ..However, distinct from other ASAP family members, AGAP1 also induced the loss of actin stress fibers. Thus, AGAP1 is a phosphoinositide-dependent Arf GAP that impacts both the endocytic compartment and actin...
  30. doi request reprint In vitro and in vivo analysis of neurotrophin-3 activation of Arf6 and Rac-1
    Pedro F Esteban
    Neural Development Group, Mouse Cancer Genetics Program, National Cancer Institute, Frederick, Maryland, USA
    Methods Enzymol 438:171-83. 2008
    ..Here we describe the rationale and protocols used for the dissection of an NT3 activated pathway that leads to the specific activation of Arf6 and Rac1...
  31. ncbi request reprint Arf regulates interaction of GGA with mannose-6-phosphate receptor
    Dianne Snow Hirsch
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Bldg 37 Room 6032 and Cell Biology and Metabolism Branch, National Institute of Child Health and Development, National Institutes of Health, Bethesda MD 20892, USA
    Traffic 4:26-35. 2003
    ..GGA and mannose-6-phosphate receptor are then incorporated into a transport intermediate that excludes Arf...
  32. ncbi request reprint Arf1 dissociates from the clathrin adaptor GGA prior to being inactivated by Arf GTPase-activating proteins
    Kerry M Jacques
    Laboratory of Cellular Oncology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:47235-41. 2002
    ..Instead, termination of Arf1 signals mediated through GGA require that Arf1.GTP dissociates from GGA prior to interaction with GAP and consequent hydrolysis of GTP...
  33. ncbi request reprint Specific regulation of the adaptor protein complex AP-3 by the Arf GAP AGAP1
    Zhongzhen Nie
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Building 37, Room 4118, Bethesda, MD 20892, USA
    Dev Cell 5:513-21. 2003
    ..Cells overexpressing AGAP1 also exhibit increased LAMP1 trafficking via the plasma membrane. Taken together, these results support the hypothesis that AGAP1 directly and specifically regulates AP-3-dependent trafficking...
  34. ncbi request reprint In vitro assays of Arf1 interaction with GGA proteins
    Hye Young Yoon
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    Methods Enzymol 404:316-32. 2005
    ..In this chapter, we describe in vitro assays for analysis of GGA interaction with Arf1 x GTP and for determining intracellular Arf1 x GTP levels...
  35. pmc Semaphorin 3E initiates antiangiogenic signaling through plexin D1 by regulating Arf6 and R-Ras
    Atsuko Sakurai
    Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Drive, Rm 211, MSC 4340, Bethesda, MD 20892, USA
    Mol Cell Biol 30:3086-98. 2010
    ..Ultimately, our present study provides a molecular framework for antiangiogenesis signaling, thus impinging on a myriad of pathological conditions that are characterized by aberrant increase in neovessel formation, including cancer...
  36. pmc GTP-binding protein-like domain of AGAP1 is protein binding site that allosterically regulates ArfGAP protein catalytic activity
    RuiBai Luo
    From the Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    J Biol Chem 287:17176-85. 2012
    ..In contrast, a C-terminal peptide from Cdc42 neither bound nor activated AGAP1. Based on these results, we propose that AGAPs are allosterically regulated through protein binding to the GLD domain...
  37. ncbi request reprint Assays and properties of the Arf GAPs AGAP1, ASAP1, and Arf GAP1
    Magnus Mutah Che
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    Methods Enzymol 404:147-63. 2005
    ..Conditions that optimize activity for each GAP are discussed. These methods can be used for the further characterization of Arf-Arf GAP interaction that is necessary for understanding the function of Arf in cellular physiology...
  38. ncbi request reprint Arf and its many interactors
    Zhongzhen Nie
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Building 37, Room 4118, Bethesda, MD 20892, USA
    Curr Opin Cell Biol 15:396-404. 2003
    ..Recent work examining the functional relationships among the diverse Arf interactors has led to reconsideration of the prevailing paradigms for Arf action...
  39. ncbi request reprint RhoD, Src, and hDia2C in endosome motility
    Paul A Randazzo
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Dev Cell 4:287-8. 2003
    ..A recent report from Marino Zerial and colleagues examining RhoD identifies mDia2C, a novel splice variant of mDia2, and Src as components of the regulatory machinery influencing actin-dependent endosome movement...
  40. pmc The pleckstrin homology (PH) domain of the Arf exchange factor Brag2 is an allosteric binding site
    Xiaoying Jian
    Laboratory of Cellular and Molecular Biology, Center for Cancer Research, NCI, NHLBI, National Institutes of Health Bethesda, Maryland 20892, USA
    J Biol Chem 287:24273-83. 2012
    ..Thus, the PH domain and the interdomain linker of Brag2 may be targets for selectively regulating the activity of Brag2...
  41. ncbi request reprint Arf GAPs and membrane traffic
    Zhongzhen Nie
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA
    J Cell Sci 119:1203-11. 2006
    ..These results are the primary basis for modifications to the classical model for the function of Arf in transport vesicle formation, including a recent proposal that Arf has a proofreading, rather than a structural, role...
  42. pmc Arf GTPase-activating proteins and their potential role in cell migration and invasion
    Fanny Campa
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892 4256, USA
    Cell Adh Migr 2:258-62. 2008
    ..In this commentary, we discuss examples of Arf GAPs that function either as regulators of Arfs or independently of the GTPase activity to regulate membrane structures that mediate cell adhesion and movement...
  43. ncbi request reprint Preparation of myristoylated Arf1 and Arf6
    Vi Luan Ha
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    Methods Enzymol 404:164-74. 2005
    ..Here, we describe methods that yield homogeneous preparations of myristoylated Arf1 and Arf6...
  44. pmc ACAPs are arf6 GTPase-activating proteins that function in the cell periphery
    T R Jackson
    Laboratory of Cellular Oncology, Division of Basic Sciences, National Cancer Institute, Bethesda, Maryland 20892 4255, USA
    J Cell Biol 151:627-38. 2000
    ..The additional effects of ASAP1 on PDGF-induced ruffling in fibroblasts suggest that multiple Arf GAPs function coordinately in the cell periphery...
  45. doi request reprint Approaches to studying Arf GAPs in cells: in vitro assay with isolated focal adhesions
    Pei wen Chen
    Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA
    Curr Protoc Cell Biol . 2012
    ..Detailed protocols for examining the activity of Arf GAPs in whole cell lysates and in association with isolated focal adhesions are provided...
  46. pmc GEFH1 binds ASAP1 and regulates podosome formation
    Yoko Shiba
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bldg 37 Room 2042, Bethesda, MD 20892, United States
    Biochem Biophys Res Commun 406:574-9. 2011
    ..Reduced expression of GEFH1, achieved with siRNA treatment, did not affect matrix degradation by podosomes but increased the rate of podosome assembly. Based on these results, we conclude that GEFH1 is a negative regulator of podosomes...
  47. ncbi request reprint ARAP1: a point of convergence for Arf and Rho signaling
    Koichi Miura
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Mol Cell 9:109-19. 2002
    ..The Arf GAP and Rho GAP activities both contributed to inhibiting cell spreading. Thus, ARAP1 is a PIP3-dependent Arf GAP that regulates Arf-, Rho-, and Cdc42-dependent cell activities...
  48. pmc A kinase-deficient TrkC receptor isoform activates Arf6-Rac1 signaling through the scaffold protein tamalin
    Pedro F Esteban
    Neural Development Group, Mouse Cancer Genetics Program, National Cancer Institute, Frederick, MD 21702, USA
    J Cell Biol 173:291-9. 2006
    ..Thus, our data identify a new signaling pathway elicited by the kinase-deficient TrkCT1 receptor. Moreover, we establish NT3 as an upstream regulator of Arf6...
  49. ncbi request reprint Effects of acid phospholipids on ARF activities: potential roles in membrane traffic
    R A Kahn
    Laboratory of Biological Chemistry, DTP, DCT, National Cancer Institute, NIH, Bethesda MD 20892, USA
    J Lipid Mediat Cell Signal 14:209-14. 1996
    ..There are potentially two distinct phospholipid binding sites each of which are coupled to the nucleotide binding site of ARFs...
  50. ncbi request reprint ArfGAP1 function in COPI mediated membrane traffic: currently debated models and comparison to other coat-binding ArfGAPs
    Yoko Shiba
    National Cancer Institute, Laboratory of Cellular and Molecular Biology, Bethesda, MD 20892, USA
    Histol Histopathol 27:1143-53. 2012
    ..We briefly discuss other ArfGAPs that may have similar function in Arf-dependent membrane traffic outside the ER-Golgi...
  51. pmc ASAP1, a phospholipid-dependent arf GTPase-activating protein that associates with and is phosphorylated by Src
    M T Brown
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Mol Cell Biol 18:7038-51. 1998
    ..By directly interacting with both Arfs and tyrosine kinases involved in regulating cell growth and cytoskeletal organization, ASAP1 could coordinate membrane remodeling events with these processes...
  52. ncbi request reprint Preparation of myristoylated Arf1 and Arf6 proteins
    Paul A Randazzo
    Laboratory of Cellular Oncology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 189:169-79. 2002
  53. pmc Resolution of two ADP-ribosylation factor 1 GTPase-activating proteins from rat liver
    P A Randazzo
    Laboratory of Biological Chemistry, Division of Basic Sciences, National Cancer Institute, Bldg 37, Rm 5D 02, 37 Convent Dr MSC 4255, Bethesda, MD 20892 4255, USA
    Biochem J 324:413-9. 1997
    ..Both GAP1 and GAP2 used ARF1 and ARF5 as substrates but not ARF6 or ARF-like protein-2. The potential role of multiple ARF GAPs in the independent regulation of ARF at specific steps in membrane traffic is discussed...
  54. ncbi request reprint The GGAs promote ARF-dependent recruitment of clathrin to the TGN
    R Puertollano
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Cell 105:93-102. 2001
    ..These observations suggest that the GGAs could function to link clathrin to membrane-bound ARF.GTP...
  55. ncbi request reprint Mutational analysis of Saccharomyces cerevisiae ARF1
    R A Kahn
    Laboratory of Biological Chemistry, NCI, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 270:143-50. 1995
    ..Two high-copy suppressors of this conditional phenotype were cloned and sequenced. One of these suppressors, SFS4, was found to be identical to PBS2/HOG4, recently shown to encode a microtubule-associated protein kinase kinase in yeast...
  56. ncbi request reprint ADP-ribosylation factor (ARF)-like 3, a new member of the ARF family of GTP-binding proteins cloned from human and rat tissues
    M M Cavenagh
    Laboratory of Biological Chemistry, NCI, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 269:18937-42. 1994
    ..Thus, Arl3 is a ubiquitously expressed GTP-binding protein in the ARF family with distinctive biochemical properties consistent with its having unique, but unknown, role(s) in cell physiology...
  57. pmc Rac inhibits thrombin-induced Rho activation: evidence of a Pak-dependent GTPase crosstalk
    Hans Rosenfeldt
    Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, DHHS, Bethesda, Maryland 20892 4330, USA
    J Mol Signal 1:8. 2006
    ..These findings indicate that Rac interferes with receptor-dependent Rho stimulation through Pak1, thus providing a mechanism for cross-talk between these two small-GTPases...
  58. pmc ACAP-A/B are ArfGAP homologs in dictyostelium involved in sporulation but not in chemotaxis
    Pei wen Chen
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, Maryland, United States of America
    PLoS ONE 5:e8624. 2010
    ..The absence of an effect on Dictyostelium migration may be due to a specific requirement for ACAPs in mesenchymal migration, which is observed in epithelial cancer cells where most studies of mammalian ArfGAPs were performed...
  59. pmc Activity of specific lipid-regulated ADP ribosylation factor-GTPase-activating proteins is required for Sec14p-dependent Golgi secretory function in yeast
    Lora L Yanagisawa
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, Alabama 35294 0005, USA
    Mol Biol Cell 13:2193-206. 2002
    ....
  60. ncbi request reprint ARAP3 is transiently tyrosine phosphorylated in cells attaching to fibronectin and inhibits cell spreading in a RhoGAP-dependent manner
    Stacey T T I
    Department of Surgery, University of Melbourne, Level 5 Clinical Sciences Building, Royal Melbourne Hospital, VIC 3050, Australia
    J Cell Sci 117:6071-84. 2004
    ..These results implicate ARAP3 in integrin-mediated tyrosine kinase signalling pathways controlling Rho GTPases and cell spreading...
  61. pmc ARFGAP1 promotes the formation of COPI vesicles, suggesting function as a component of the coat
    Jia Shu Yang
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 159:69-78. 2002
    ..Collectively, these findings suggest that ARFGAP1 promotes vesicle formation by functioning as a component of the COPI coat...
  62. pmc CIN85 associates with multiple effectors controlling intracellular trafficking of epidermal growth factor receptors
    Katarzyna Kowanetz
    Institute of Biochemistry II, Goethe University Medical School, 60590 Frankfurt, Germany
    Mol Biol Cell 15:3155-66. 2004
    ..We propose that CIN85 functions as a scaffold molecule that binds to numerous endocytic accessory proteins, thus controlling distinct steps in trafficking of EGF receptors along the endocytic and recycling pathways...