Genomes and Genes
Affiliation: National Institutes of Health
- Amplified expression profiling of platelet transcriptome reveals changes in arginine metabolic pathways in patients with sickle cell diseaseNalini Raghavachari
Vascular Medicine Branch, National Heart, Lung, and Blood Institute National Institutes of Health, Bethesda, MD 20892 1454, USA
Circulation 115:1551-62. 2007..Recent functional studies of platelets in patients with sickle cell disease reveal a basally activated state, which suggests that pathological platelet activation may contribute to sickle cell disease vasculopathy...
- A systematic comparison and evaluation of high density exon arrays and RNA-seq technology used to unravel the peripheral blood transcriptome of sickle cell diseaseNalini Raghavachari
Genomics Core Facility, Genetics and Development Biology, NHLBI, The National Institutes of Health, 10 Center Drive, Bldg 10, 8C 103B, Bethesda, MD 20892, USA
BMC Med Genomics 5:28. 2012..The field of transcriptomics is currently being revolutionized by high throughput DNA sequencing methodologies to map, characterize, and quantify the transcriptome...
- Characterization of whole blood gene expression profiles as a sequel to globin mRNA reduction in patients with sickle cell diseaseNalini Raghavachari
Pulmonary and Vascular Medicine Branch, National Heart Lung and Blood Institute, NIH, Bethesda, MD, USA
PLoS ONE 4:e6484. 2009..The combination of globin mRNA reduction after whole-blood RNA stabilization represents a robust clinical research methodology for the discovery of biomarkers for hematologic diseases...
- Transcriptional profiling of CD133(+) cells in coronary artery disease and effects of exercise on gene expressionDelong Liu
Genomics Core, National Institutes of Health, Bethesda, Maryland, USA
Cytotherapy 13:227-36. 2011..Our aim was to identify differences in gene expression in CD133(+) cells of patients with coronary artery disease (CAD) and healthy controls, and determine whether exercise modifies gene expression...
- Transcriptome profiling and sequencing of differentiated human hematopoietic stem cells reveal lineage-specific expression and alternative splicing of genesPoching Liu
Genomics Core Facility, Genetics and Development Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Physiol Genomics 43:1117-34. 2011..Knowledge of molecular processes by which the cells acquire their cell-specific fate would be beneficial in developing cell-based therapies for human diseases...
- Treatment-induced oxidative stress and cellular antioxidant capacity determine response to bortezomib in mantle cell lymphomaMarc A Weniger
Hematology Branch, NHLBI, NIH, Building 10, CRC 3 5140, 10 Center Drive, Bethesda, 20892 MD, USA
Clin Cancer Res 17:5101-12. 2011..Up to 50% of patients with relapsed mantle cell lymphoma (MCL) respond to bortezomib. We used gene expression profiling to investigate the connection between proteasome inhibition, cellular response, and clinical efficacy...
- Microarray-based characterization of a colony assay used to investigate endothelial progenitor cells and relevance to endothelial function in humansAditi Desai
Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1454, USA
Arterioscler Thromb Vasc Biol 29:121-7. 2009..An assay proposed to quantify endothelial progenitor cell (EPC) colonies in humans was investigated to determine the phenotype of recovered cells and their relevance to in vivo endothelial function...
- CD146+ T lymphocytes are increased in both the peripheral circulation and in the synovial effusions of patients with various musculoskeletal diseases and display pro-inflammatory gene profilesPradeep Kumar Dagur
Flow Cytometry Core Facility, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892, USA
Cytometry B Clin Cytom 78:88-95. 2010..Our study establishes increased CD146+ T-lymphocytes in diseases with joint effusions, and demonstrates pro-inflammatory gene profiles in these cells...
- Bone marrow-derived cells do not repair endothelium in a mouse model of chronic endothelial cell dysfunctionTashera E Perry
Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, 9000 Rockville Pike, Building 10 CRC, Room 6 3130, Bethesda, MD 20892, USA
Cardiovasc Res 84:317-25. 2009..We assessed the hypothesis that a BM transplant from healthy animals would restore normal arterial endothelium and prevent hypertension in young endothelial nitric oxide synthase-deficient (eNOS(-/-)) mice...
- A unique population of effector memory lymphocytes identified by CD146 having a distinct immunophenotypic and genomic profileMohamed F Elshal
Flow Cytometry Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
BMC Immunol 8:29. 2007..In the current study, lymphocytes from healthy donors were characterized based upon the presence or absence of CD146 expression...
- Phenotypic and functional characterization of a mouse model of targeted Pig-a deletion in hematopoietic cellsValeria Visconte
Hematology Branch, NHLBI, National Institutes of Health, Bethesda, MD 20892 1202 USA
Haematologica 95:214-23. 2010..Somatic mutation in the X-linked phosphatidylinositol glycan class A gene (PIG-A) causes glycosyl phosphatidylinositol anchor deficiency in human patients with paroxysmal nocturnal hemoglobinuria...
- Bortezomib resistance in mantle cell lymphoma is associated with plasmacytic differentiationPatricia Perez-Galan
Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA
Blood 117:542-52. 2011..Expression of CD38 and IRF4 could serve as markers of bortezomib resistance in MCL. This study has been registered at http://clinicaltrials.gov as NCT00131976...
- Dietary nitrate and nitrite modulate blood and organ nitrite and the cellular ischemic stress responseNicolaas J H Raat
Pulmonary and Vascular Medicine Branch, National Heart, Lung, and Blood Institute, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
Free Radic Biol Med 47:510-7. 2009....
- Rabbit ATG but not horse ATG promotes expansion of functional CD4+CD25highFOXP3+ regulatory T cells in vitroXingmin Feng
Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Blood 111:3675-83. 2008..The therapeutic effects of rATG may occur not only because of lymphocyte depletion but also enhanced Treg cell number and function...
- Apolipoprotein E negatively regulates house dust mite-induced asthma via a low-density lipoprotein receptor-mediated pathwayXianglan Yao
Pulmonary and Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1590, USA
Am J Respir Crit Care Med 182:1228-38. 2010..Distinct sets of corticosteroid-unresponsive genes modulate disease severity in asthma...
- The lymph node microenvironment promotes B-cell receptor signaling, NF-kappaB activation, and tumor proliferation in chronic lymphocytic leukemiaYair Herishanu
National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA
Blood 117:563-74. 2011..These data identify the disruption of tumor microenvironment interactions and the inhibition of BCR signaling as promising therapeutic strategies in CLL. This study is registered at http://clinicaltrials.gov as NCT00019370...
- Human parvovirus B19 causes cell cycle arrest of human erythroid progenitors via deregulation of the E2F family of transcription factorsZhihong Wan
Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland 20892 1652, USA
J Clin Invest 120:3530-44. 2010..These findings provide new insight into the molecular pathogenesis of B19V in highly permissive erythroid progenitors...
- MicroRNA expression in human retinal pigment epithelial (ARPE-19) cells: increased expression of microRNA-9 by N-(4-hydroxyphenyl)retinamideR Krishnan Kutty
Laboratory of Retinal Cell and Molecular Biology, Bldg 6, Room 112, National Eye Institute, National Institutes of Health, 6 Center Dr, MSC 0608, Bethesda, MD 20892, USA
Mol Vis 16:1475-86. 2010..The aim of the present study was to investigate the expression of miR-9 in ARPE-19 cells in response to 4HPR treatment, and to identify other miRNAs normally expressed in these cells...
- Blood mononuclear cell gene expression profiles characterize the oxidant, hemolytic, and inflammatory stress of sickle cell diseaseMaria L Jison
Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
Blood 104:270-80. 2004..Global transcriptional analysis of circulating leukocytes highlights the intense oxidant and inflammatory nature of steady-state sickle cell disease and provides insight into the broad compensatory responses to vascular injury...
- Circulating blood endothelial nitric oxide synthase contributes to the regulation of systemic blood pressure and nitrite homeostasisKatherine C Wood
Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
Arterioscler Thromb Vasc Biol 33:1861-71. 2013..A functional red cell eNOS that modulates vascular NO• signaling has been proposed...
- PGC-1alpha integrates insulin signaling, mitochondrial regulation, and bioenergetic function in skeletal muscleInes Pagel-Langenickel
Translational Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1454, USA
J Biol Chem 283:22464-72. 2008..Pioglitazone functions in part via the induction of PGC-1alpha. Moreover, PGC-1alpha is identified as a bidirectional regulatory link integrating insulin-signaling and mitochondrial homeostasis in skeletal muscle...
- Heme oxygenase-1 deficiency accelerates formation of arterial thrombosis through oxidative damage to the endothelium, which is rescued by inhaled carbon monoxideAndrea L True
National Heart, Lung and Blood Institute, NIH, Bethesda, MD 20892, USA
Circ Res 101:893-901. 2007..Induction of HO-1 may be beneficial in the prevention of thrombosis associated with vascular oxidant stress and inflammation...
- miR-222 contributes to sex-dimorphic cardiac eNOS expression via ets-1Alicia M Evangelista
Systems Biology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Physiol Genomics 45:493-8. 2013..Our findings indicate that sex-dependent miR-222 regulation alters the expression of the cardiac regulatory protein eNOS...