Nalini Raghavachari

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Amplified expression profiling of platelet transcriptome reveals changes in arginine metabolic pathways in patients with sickle cell disease
    Nalini Raghavachari
    Vascular Medicine Branch, National Heart, Lung, and Blood Institute National Institutes of Health, Bethesda, MD 20892 1454, USA
    Circulation 115:1551-62. 2007
  2. pmc A systematic comparison and evaluation of high density exon arrays and RNA-seq technology used to unravel the peripheral blood transcriptome of sickle cell disease
    Nalini Raghavachari
    Genomics Core Facility, Genetics and Development Biology, NHLBI, The National Institutes of Health, 10 Center Drive, Bldg 10, 8C 103B, Bethesda, MD 20892, USA
    BMC Med Genomics 5:28. 2012
  3. pmc Characterization of whole blood gene expression profiles as a sequel to globin mRNA reduction in patients with sickle cell disease
    Nalini Raghavachari
    Pulmonary and Vascular Medicine Branch, National Heart Lung and Blood Institute, NIH, Bethesda, MD, USA
    PLoS ONE 4:e6484. 2009
  4. pmc Transcriptional profiling of CD133(+) cells in coronary artery disease and effects of exercise on gene expression
    Delong Liu
    Genomics Core, National Institutes of Health, Bethesda, Maryland, USA
    Cytotherapy 13:227-36. 2011
  5. pmc Transcriptome profiling and sequencing of differentiated human hematopoietic stem cells reveal lineage-specific expression and alternative splicing of genes
    Poching Liu
    Genomics Core Facility, Genetics and Development Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Physiol Genomics 43:1117-34. 2011
  6. pmc Treatment-induced oxidative stress and cellular antioxidant capacity determine response to bortezomib in mantle cell lymphoma
    Marc A Weniger
    Hematology Branch, NHLBI, NIH, Building 10, CRC 3 5140, 10 Center Drive, Bethesda, 20892 MD, USA
    Clin Cancer Res 17:5101-12. 2011
  7. pmc Microarray-based characterization of a colony assay used to investigate endothelial progenitor cells and relevance to endothelial function in humans
    Aditi Desai
    Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1454, USA
    Arterioscler Thromb Vasc Biol 29:121-7. 2009
  8. pmc Circulating blood endothelial nitric oxide synthase contributes to the regulation of systemic blood pressure and nitrite homeostasis
    Katherine C Wood
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Arterioscler Thromb Vasc Biol 33:1861-71. 2013
  9. pmc Integrated analysis of miRNA and mRNA during differentiation of human CD34+ cells delineates the regulatory roles of microRNA in hematopoiesis
    Nalini Raghavachari
    Genomics Core Facility, Genetics and Development Biology, National Heart, Lung, and Blood Institute, Bethesda, MD, USA Geriatrics and Clinical Geronotology, National Institutes of Health, Bethesda, MD, USA Electronic address
    Exp Hematol 42:14-27.e1-2. 2014
  10. pmc CD146+ T lymphocytes are increased in both the peripheral circulation and in the synovial effusions of patients with various musculoskeletal diseases and display pro-inflammatory gene profiles
    Pradeep Kumar Dagur
    Flow Cytometry Core Facility, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892, USA
    Cytometry B Clin Cytom 78:88-95. 2010

Collaborators

Detail Information

Publications28

  1. pmc Amplified expression profiling of platelet transcriptome reveals changes in arginine metabolic pathways in patients with sickle cell disease
    Nalini Raghavachari
    Vascular Medicine Branch, National Heart, Lung, and Blood Institute National Institutes of Health, Bethesda, MD 20892 1454, USA
    Circulation 115:1551-62. 2007
    ..Recent functional studies of platelets in patients with sickle cell disease reveal a basally activated state, which suggests that pathological platelet activation may contribute to sickle cell disease vasculopathy...
  2. pmc A systematic comparison and evaluation of high density exon arrays and RNA-seq technology used to unravel the peripheral blood transcriptome of sickle cell disease
    Nalini Raghavachari
    Genomics Core Facility, Genetics and Development Biology, NHLBI, The National Institutes of Health, 10 Center Drive, Bldg 10, 8C 103B, Bethesda, MD 20892, USA
    BMC Med Genomics 5:28. 2012
    ..The field of transcriptomics is currently being revolutionized by high throughput DNA sequencing methodologies to map, characterize, and quantify the transcriptome...
  3. pmc Characterization of whole blood gene expression profiles as a sequel to globin mRNA reduction in patients with sickle cell disease
    Nalini Raghavachari
    Pulmonary and Vascular Medicine Branch, National Heart Lung and Blood Institute, NIH, Bethesda, MD, USA
    PLoS ONE 4:e6484. 2009
    ..The combination of globin mRNA reduction after whole-blood RNA stabilization represents a robust clinical research methodology for the discovery of biomarkers for hematologic diseases...
  4. pmc Transcriptional profiling of CD133(+) cells in coronary artery disease and effects of exercise on gene expression
    Delong Liu
    Genomics Core, National Institutes of Health, Bethesda, Maryland, USA
    Cytotherapy 13:227-36. 2011
    ..Our aim was to identify differences in gene expression in CD133(+) cells of patients with coronary artery disease (CAD) and healthy controls, and determine whether exercise modifies gene expression...
  5. pmc Transcriptome profiling and sequencing of differentiated human hematopoietic stem cells reveal lineage-specific expression and alternative splicing of genes
    Poching Liu
    Genomics Core Facility, Genetics and Development Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Physiol Genomics 43:1117-34. 2011
    ..Knowledge of molecular processes by which the cells acquire their cell-specific fate would be beneficial in developing cell-based therapies for human diseases...
  6. pmc Treatment-induced oxidative stress and cellular antioxidant capacity determine response to bortezomib in mantle cell lymphoma
    Marc A Weniger
    Hematology Branch, NHLBI, NIH, Building 10, CRC 3 5140, 10 Center Drive, Bethesda, 20892 MD, USA
    Clin Cancer Res 17:5101-12. 2011
    ..Up to 50% of patients with relapsed mantle cell lymphoma (MCL) respond to bortezomib. We used gene expression profiling to investigate the connection between proteasome inhibition, cellular response, and clinical efficacy...
  7. pmc Microarray-based characterization of a colony assay used to investigate endothelial progenitor cells and relevance to endothelial function in humans
    Aditi Desai
    Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1454, USA
    Arterioscler Thromb Vasc Biol 29:121-7. 2009
    ..An assay proposed to quantify endothelial progenitor cell (EPC) colonies in humans was investigated to determine the phenotype of recovered cells and their relevance to in vivo endothelial function...
  8. pmc Circulating blood endothelial nitric oxide synthase contributes to the regulation of systemic blood pressure and nitrite homeostasis
    Katherine C Wood
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Arterioscler Thromb Vasc Biol 33:1861-71. 2013
    ..A functional red cell eNOS that modulates vascular NO• signaling has been proposed...
  9. pmc Integrated analysis of miRNA and mRNA during differentiation of human CD34+ cells delineates the regulatory roles of microRNA in hematopoiesis
    Nalini Raghavachari
    Genomics Core Facility, Genetics and Development Biology, National Heart, Lung, and Blood Institute, Bethesda, MD, USA Geriatrics and Clinical Geronotology, National Institutes of Health, Bethesda, MD, USA Electronic address
    Exp Hematol 42:14-27.e1-2. 2014
    ..We believe that this study will enhance our understanding on the regulatory roles of miRNA in hematopoiesis by providing a library of mRNA-miRNA networks. ..
  10. pmc CD146+ T lymphocytes are increased in both the peripheral circulation and in the synovial effusions of patients with various musculoskeletal diseases and display pro-inflammatory gene profiles
    Pradeep Kumar Dagur
    Flow Cytometry Core Facility, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892, USA
    Cytometry B Clin Cytom 78:88-95. 2010
    ..Our study establishes increased CD146+ T-lymphocytes in diseases with joint effusions, and demonstrates pro-inflammatory gene profiles in these cells...
  11. pmc Imaging flow cytometry for morphologic and phenotypic characterization of rare circulating endothelial cells
    Leigh Samsel
    Flow Cytometry Core Facility, National Heart Lung and Blood Institute, NIH, Bethesda, Maryland
    Cytometry B Clin Cytom 84:379-89. 2013
    ..Thus, this method will enable a broad range of novel studies to be conducted using CECs as surrogates of the endothelium...
  12. pmc Bone marrow-derived cells do not repair endothelium in a mouse model of chronic endothelial cell dysfunction
    Tashera E Perry
    Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, 9000 Rockville Pike, Building 10 CRC, Room 6 3130, Bethesda, MD 20892, USA
    Cardiovasc Res 84:317-25. 2009
    ..We assessed the hypothesis that a BM transplant from healthy animals would restore normal arterial endothelium and prevent hypertension in young endothelial nitric oxide synthase-deficient (eNOS(-/-)) mice...
  13. pmc A unique population of effector memory lymphocytes identified by CD146 having a distinct immunophenotypic and genomic profile
    Mohamed F Elshal
    Flow Cytometry Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    BMC Immunol 8:29. 2007
    ..In the current study, lymphocytes from healthy donors were characterized based upon the presence or absence of CD146 expression...
  14. pmc Phenotypic and functional characterization of a mouse model of targeted Pig-a deletion in hematopoietic cells
    Valeria Visconte
    Hematology Branch, NHLBI, National Institutes of Health, Bethesda, MD 20892 1202 USA
    Haematologica 95:214-23. 2010
    ..Somatic mutation in the X-linked phosphatidylinositol glycan class A gene (PIG-A) causes glycosyl phosphatidylinositol anchor deficiency in human patients with paroxysmal nocturnal hemoglobinuria...
  15. pmc Bortezomib resistance in mantle cell lymphoma is associated with plasmacytic differentiation
    Patricia Perez-Galan
    Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Blood 117:542-52. 2011
    ..Expression of CD38 and IRF4 could serve as markers of bortezomib resistance in MCL. This study has been registered at http://clinicaltrials.gov as NCT00131976...
  16. pmc miR-222 contributes to sex-dimorphic cardiac eNOS expression via ets-1
    Alicia M Evangelista
    Systems Biology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Physiol Genomics 45:493-8. 2013
    ..Our findings indicate that sex-dependent miR-222 regulation alters the expression of the cardiac regulatory protein eNOS...
  17. pmc The lymph node microenvironment promotes B-cell receptor signaling, NF-kappaB activation, and tumor proliferation in chronic lymphocytic leukemia
    Yair Herishanu
    National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Blood 117:563-74. 2011
    ..These data identify the disruption of tumor microenvironment interactions and the inhibition of BCR signaling as promising therapeutic strategies in CLL. This study is registered at http://clinicaltrials.gov as NCT00019370...
  18. pmc Apolipoprotein E negatively regulates house dust mite-induced asthma via a low-density lipoprotein receptor-mediated pathway
    Xianglan Yao
    Pulmonary and Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1590, USA
    Am J Respir Crit Care Med 182:1228-38. 2010
    ..Distinct sets of corticosteroid-unresponsive genes modulate disease severity in asthma...
  19. pmc Dietary nitrate and nitrite modulate blood and organ nitrite and the cellular ischemic stress response
    Nicolaas J H Raat
    Pulmonary and Vascular Medicine Branch, National Heart, Lung, and Blood Institute, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 47:510-7. 2009
    ....
  20. pmc Rabbit ATG but not horse ATG promotes expansion of functional CD4+CD25highFOXP3+ regulatory T cells in vitro
    Xingmin Feng
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 111:3675-83. 2008
    ..The therapeutic effects of rATG may occur not only because of lymphocyte depletion but also enhanced Treg cell number and function...
  21. doi request reprint Microarray technology: basic methodology and application in clinical research for biomarker discovery in vascular diseases
    Nalini Raghavachari
    Genetics and Developmental Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 1027:47-84. 2013
    ..This chapter will provide details on the methodologies that address these problems for successful microarray-based transcriptome analysis of tissues, whole blood, cell subpopulations, and cultured cells. ..
  22. pmc A role for phosphodiesterase 3B in acquisition of brown fat characteristics by white adipose tissue in male mice
    Emilia Guirguis
    Pulmonary Cardiovascular Branch, National Institutes of Health, Bethesda, Maryland 20892, USA
    Endocrinology 154:3152-67. 2013
    ....
  23. pmc MicroRNA expression in human retinal pigment epithelial (ARPE-19) cells: increased expression of microRNA-9 by N-(4-hydroxyphenyl)retinamide
    R Krishnan Kutty
    Laboratory of Retinal Cell and Molecular Biology, Bldg 6, Room 112, National Eye Institute, National Institutes of Health, 6 Center Dr, MSC 0608, Bethesda, MD 20892, USA
    Mol Vis 16:1475-86. 2010
    ..The aim of the present study was to investigate the expression of miR-9 in ARPE-19 cells in response to 4HPR treatment, and to identify other miRNAs normally expressed in these cells...
  24. pmc Human parvovirus B19 causes cell cycle arrest of human erythroid progenitors via deregulation of the E2F family of transcription factors
    Zhihong Wan
    Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland 20892 1652, USA
    J Clin Invest 120:3530-44. 2010
    ..These findings provide new insight into the molecular pathogenesis of B19V in highly permissive erythroid progenitors...
  25. ncbi request reprint The very low density lipoprotein receptor attenuates house dust mite-induced airway inflammation by suppressing dendritic cell-mediated adaptive immune responses
    Karin Fredriksson
    Laboratory of Asthma and Lung Inflammation, Cardiovascular and Pulmonary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892
    J Immunol 192:4497-509. 2014
    ..Collectively, these results identify a novel role for the VLDLR as a negative regulator of DC-mediated adaptive immune responses in HDM-induced allergic airway inflammation. ..
  26. ncbi request reprint Blood mononuclear cell gene expression profiles characterize the oxidant, hemolytic, and inflammatory stress of sickle cell disease
    Maria L Jison
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 104:270-80. 2004
    ..Global transcriptional analysis of circulating leukocytes highlights the intense oxidant and inflammatory nature of steady-state sickle cell disease and provides insight into the broad compensatory responses to vascular injury...
  27. pmc PGC-1alpha integrates insulin signaling, mitochondrial regulation, and bioenergetic function in skeletal muscle
    Ines Pagel-Langenickel
    Translational Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1454, USA
    J Biol Chem 283:22464-72. 2008
    ..Pioglitazone functions in part via the induction of PGC-1alpha. Moreover, PGC-1alpha is identified as a bidirectional regulatory link integrating insulin-signaling and mitochondrial homeostasis in skeletal muscle...
  28. ncbi request reprint Heme oxygenase-1 deficiency accelerates formation of arterial thrombosis through oxidative damage to the endothelium, which is rescued by inhaled carbon monoxide
    Andrea L True
    National Heart, Lung and Blood Institute, NIH, Bethesda, MD 20892, USA
    Circ Res 101:893-901. 2007
    ..Induction of HO-1 may be beneficial in the prevention of thrombosis associated with vascular oxidant stress and inflammation...