J W Putney

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1
    Jason C Mercer
    Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 281:24979-90. 2006
  2. ncbi request reprint Pharmacology of capacitative calcium entry
    J W Putney
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, PO Box 12233, Research Triangle Park, NC 27709, USA
    Mol Interv 1:84-94. 2001
  3. ncbi request reprint Calcium signaling in lacrimal glands
    James W Putney
    Calcium Regulation Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, Department of Health and Human Services, PO Box 12233, Research Triangle Park, NC 27709, USA Electronic address
    Cell Calcium 55:290-6. 2014
  4. doi request reprint Role of Orai1 and store-operated calcium entry in mouse lacrimal gland signalling and function
    Juan Xing
    NIEHS, NIH Chief Calcium Regulation Section, PO Box 12233, 111 Alexander Drive F255, Research Triangle Park, NC 27709 2233, USA
    J Physiol 592:927-39. 2014
  5. doi request reprint Calcium signaling: septins organize the SOC channel
    James W Putney
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Curr Biol 23:R684-5. 2013
  6. doi request reprint Alternative forms of the store-operated calcium entry mediators, STIM1 and Orai1
    James W Putney
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, Department of Health and Human Services, Durham, North Carolina, USA
    Curr Top Membr 71:109-23. 2013
  7. doi request reprint Calcium signaling: deciphering the calcium-NFAT pathway
    James W Putney
    National Institute of Environmental Health Sciences NIH, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Curr Biol 22:R87-9. 2012
  8. ncbi request reprint The enigmatic TRPCs: multifunctional cation channels
    James W Putney
    National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, PO Box 12233, Research Triangle Park, NC 27709, USA
    Trends Cell Biol 14:282-6. 2004
  9. ncbi request reprint Store-operated calcium channels: how do we measure them, and why do we care?
    James W Putney
    National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Post Office Box 12233, Research Triangle Park, NC 27709, USA
    Sci STKE 2004:pe37. 2004
  10. pmc Calcium signaling: double duty for calcium at the mitochondrial uniporter
    James W Putney
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    Curr Biol 16:R812-5. 2006

Collaborators

Detail Information

Publications80

  1. pmc Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1
    Jason C Mercer
    Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 281:24979-90. 2006
    ..However, we demonstrate that Stim1 does not incorporate in the surface membrane, and thus likely regulates or interacts with Orai1 at sites of close apposition between the plasma membrane and an intracellular Stim1-containing organelle...
  2. ncbi request reprint Pharmacology of capacitative calcium entry
    J W Putney
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, PO Box 12233, Research Triangle Park, NC 27709, USA
    Mol Interv 1:84-94. 2001
    ..Investigations into the phenomenon are contributing to a new appreciation for the organized cytoplasmic framework that supports calcium signaling...
  3. ncbi request reprint Calcium signaling in lacrimal glands
    James W Putney
    Calcium Regulation Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, Department of Health and Human Services, PO Box 12233, Research Triangle Park, NC 27709, USA Electronic address
    Cell Calcium 55:290-6. 2014
    ..Recent studies with deletions of the channel subunit, Orai1, confirm the important role of SOCE in both fluid and protein secretion in lacrimal glands, both in vivo and in vitro. ..
  4. doi request reprint Role of Orai1 and store-operated calcium entry in mouse lacrimal gland signalling and function
    Juan Xing
    NIEHS, NIH Chief Calcium Regulation Section, PO Box 12233, 111 Alexander Drive F255, Research Triangle Park, NC 27709 2233, USA
    J Physiol 592:927-39. 2014
    ..These studies indicate a critical role for Orai1-mediated SOCE in lacrimal gland signalling and function. ..
  5. doi request reprint Calcium signaling: septins organize the SOC channel
    James W Putney
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Curr Biol 23:R684-5. 2013
    ..From a whole-genome screen in HeLa cells, a new study reveals that cytoskeletal septins play a critical role in this process, by organizing signaling structures in the plasma membrane. ..
  6. doi request reprint Alternative forms of the store-operated calcium entry mediators, STIM1 and Orai1
    James W Putney
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, Department of Health and Human Services, Durham, North Carolina, USA
    Curr Top Membr 71:109-23. 2013
    ..This chapter summarizes and discusses recent work revealing alternative forms of STIM1 and Orai1, and the physiological consequences of these modified proteins. ..
  7. doi request reprint Calcium signaling: deciphering the calcium-NFAT pathway
    James W Putney
    National Institute of Environmental Health Sciences NIH, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Curr Biol 22:R87-9. 2012
    ..An elegant combination of experimental strategies and a model that encompasses non-linear inputs and outputs now sheds new light on this long-standing problem...
  8. ncbi request reprint The enigmatic TRPCs: multifunctional cation channels
    James W Putney
    National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, PO Box 12233, Research Triangle Park, NC 27709, USA
    Trends Cell Biol 14:282-6. 2004
  9. ncbi request reprint Store-operated calcium channels: how do we measure them, and why do we care?
    James W Putney
    National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Post Office Box 12233, Research Triangle Park, NC 27709, USA
    Sci STKE 2004:pe37. 2004
    ....
  10. pmc Calcium signaling: double duty for calcium at the mitochondrial uniporter
    James W Putney
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    Curr Biol 16:R812-5. 2006
    ..Recent findings reveal that the process of Ca(2+) uptake by the mitochondrial uniporter is itself regulated by Ca(2+) in a temporally complex manner...
  11. pmc Capacitative calcium entry: sensing the calcium stores
    James W Putney
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    J Cell Biol 169:381-2. 2005
    ....
  12. ncbi request reprint Physiological mechanisms of TRPC activation
    James W Putney
    Department of Health and Human Services, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, USA
    Pflugers Arch 451:29-34. 2005
    ..The TRPCs may be unique among ion-channel subunit families in being able to participate in the assembly and function of multiple types of physiologically important ion channels...
  13. pmc Recent breakthroughs in the molecular mechanism of capacitative calcium entry (with thoughts on how we got here)
    James W Putney
    National Institute of Environmental Health Sciences, NIH, P O Box 12233, Research Triangle Park, NC 27709, United States
    Cell Calcium 42:103-10. 2007
    ..Stim1, either directly or indirectly, signals to plasma membrane Orai proteins which constitute pore-forming subunits of store-operated channels...
  14. pmc New molecular players in capacitative Ca2+ entry
    James W Putney
    National Institute of Environmental Health Sciences NIH, PO Box 12233, Research Triangle Park, NC 27709, USA
    J Cell Sci 120:1959-65. 2007
    ..Stim1 signals to plasma membrane Orai proteins, which constitute pore-forming subunits of store-operated channels...
  15. pmc Cytoplasmic calcium oscillations and store-operated calcium influx
    James W Putney
    National Institute of Environmental Health Sciences, NIH, Department of Health and Human Services, PO Box 12233, Research Triangle Park, NC 27709, USA
    J Physiol 586:3055-9. 2008
    ..In this review we will discuss the role played by Ca(2+) entry mechanisms in Ca(2+) oscillations, and approaches to understanding the molecular nature of this Ca(2+) entry pathway...
  16. ncbi request reprint SOC: now also store-operated cyclase
    James W Putney
    James W Putney Jr is in the Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, Department of Health and Human Services, PO Box 12233, Research Triangle Park, NC 2770, USA
    Nat Cell Biol 11:381-2. 2009
    ..These findings substantially broaden the scope and biological significance of Ca(2+) store-regulated signalling...
  17. pmc Pharmacology of store-operated calcium channels
    James W Putney
    Calcium Regulation Section, National Institute of Environmental Health Sciences NIH, Department of Health and Human Services, PO Box 12233, Research Triangle Park, NC 27709, USA
    Mol Interv 10:209-18. 2010
    ..This review discusses recent advances in our understanding of this widespread signaling mechanism as well as the mechanisms by which a number of interesting pharmacological agents modify it...
  18. pmc The physiological function of store-operated calcium entry
    James W Putney
    National Institute of Environmental Health Sciences NIH, Department of Health and Human Services, PO Box 12233, Research Triangle Park, NC 27709, USA
    Neurochem Res 36:1157-65. 2011
    ....
  19. ncbi request reprint Origins of the concept of store-operated calcium entry
    James W Putney
    National Institute of Environmental Health Sciences NIH, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Front Biosci (Schol Ed) 3:980-4. 2011
    ..This short introduction to the current volume of Frontiers in Bioscience briefly summarizes the early experimental work that led to the idea of store-operated calcium entry, and provided the initial proofs for it...
  20. ncbi request reprint Capacitative calcium entry in the nervous system
    James W Putney
    National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Cell Calcium 34:339-44. 2003
    ..This review will summarize some of these more recent findings regarding the role of capacitative calcium entry in normal and pathological processes in the nervous system...
  21. ncbi request reprint Mechanisms of capacitative calcium entry
    J W Putney
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, NIH, Research Triangle, Park, NC 27709, USA
    J Cell Sci 114:2223-9. 2001
    ..A tentative conclusion is that multiple mechanisms for signaling capacitative Ca(2+) entry may exist, and involve conformational coupling in some cell types and perhaps a diffusible signal in others...
  22. ncbi request reprint Type 3 inositol 1,4,5-trisphosphate receptor and capacitative calcium entry
    J W Putney
    Calcium Regulation Section, National Institute of Environmental Health Sciences NIH, Research Triangle Park, North Carolina 27709, USA
    Cell Calcium 21:257-61. 1997
    ..e. trp) may carry out this function. Furthermore, as there is little, if any, homology between the IP3 and trp protein families, this may represent an example of convergent evolution of function...
  23. ncbi request reprint Signaling pathways between the plasma membrane and endoplasmic reticulum calcium stores
    J W Putney
    Calcium Regulation Section, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, Research Triangle Park, North Carolina 27709, USA
    Cell Mol Life Sci 57:1272-86. 2000
    ..The emerging view is one of a complex network of pathways through which the endoplasmic reticulum and the Ca2+ and protein kinase C signaling pathways interact at various levels regulating cellular structure and function...
  24. doi request reprint Capacitative calcium entry: from concept to molecules
    James W Putney
    Laboratory of Signal Transduction, Department of Health and Human Services, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Immunol Rev 231:10-22. 2009
    ..The process of store-operated Ca2+ entry provides support for oscillating Ca2+ signals from the ER and also provides direct activator Ca2+ that signals to a variety of downstream effectors...
  25. ncbi request reprint Activation of calcium entry by the tumor promoter thapsigargin in parotid acinar cells. Evidence that an intracellular calcium pool and not an inositol phosphate regulates calcium fluxes at the plasma membrane
    H Takemura
    Laboratory of Cellular and Molecular Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709
    J Biol Chem 264:12266-71. 1989
    ....
  26. ncbi request reprint Calcium efflux across the plasma membrane of rat parotid acinar cells is unaffected by receptor activation or by the microsomal calcium ATPase inhibitor, thapsigargin
    H Takemura
    Calcium Regulation Section, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina
    Cell Calcium 11:11-7. 1990
    ..In addition, neither methacholine nor phorbol myristate acetate decreased the sustained elevation of [Ca2+]i induced by ionomycin.(ABSTRACT TRUNCATED AT 250 WORDS)..
  27. pmc Capacitative calcium entry in parotid acinar cells
    H Takemura
    Calcium Regulation Section, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709
    Biochem J 258:409-12. 1989
    ....
  28. pmc Human Trp3 forms both inositol trisphosphate receptor-dependent and receptor-independent store-operated cation channels in DT40 avian B lymphocytes
    G Vazquez
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Proc Natl Acad Sci U S A 98:11777-82. 2001
    ..Trp3, or one of its structural relatives, is a candidate for the store-operated, nonselective cation channels observed in smooth muscle cells and other cell types...
  29. ncbi request reprint Effects of MeCh, thapsigargin, and La3+ on plasmalemmal and intracellular Ca2+ transport in lacrimal acinar cells
    C Y Kwan
    Calcium Regulation Section, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709
    Am J Physiol 258:C1006-15. 1990
    ....
  30. ncbi request reprint Role of the inositol 1,4,5-trisphosphate receptor in Ca(2+) feedback inhibition of calcium release-activated calcium current (I(crac))
    L M Broad
    Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 274:32881-8. 1999
    ..These findings reveal new and important roles for IP(3) receptors located on discrete IP(3)-sensitive Ca(2+) pools in calcium feedback regulation of I(crac) and capacitative calcium entry...
  31. ncbi request reprint Does beta-adrenoceptor activation stimulate Ca2+ mobilization and inositol trisphosphate formation in parotid acinar cells?
    A R Hughes
    Calcium Regulation Section, National Institute of Environmental Health Sciences, National Institute of Health, North Carolina
    Cell Calcium 10:519-25. 1989
    ..Thus, the effects of isoprenaline on [Ca2+]i do not appear to be mediated by cAMP.(ABSTRACT TRUNCATED AT 250 WORDS)..
  32. ncbi request reprint The role of canonical transient receptor potential 7 in B-cell receptor-activated channels
    Jean Philippe Lievremont
    Department of Health and Human Services, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 280:35346-51. 2005
    ..This represents the first demonstration of a physiological function for endogenous TRPC7 channels...
  33. pmc Role of the store-operated calcium entry proteins Stim1 and Orai1 in muscarinic cholinergic receptor-stimulated calcium oscillations in human embryonic kidney cells
    Barbara Wedel
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    J Physiol 579:679-89. 2007
    ....
  34. ncbi request reprint Role of the phospholipase C-inositol 1,4,5-trisphosphate pathway in calcium release-activated calcium current and capacitative calcium entry
    L M Broad
    Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 276:15945-52. 2001
    ..However, the results call into question the previously suggested roles of IP(3) and IP(3) receptor in this mechanism, at least in these particular cell types...
  35. ncbi request reprint A calmodulin/inositol 1,4,5-trisphosphate (IP3) receptor-binding region targets TRPC3 to the plasma membrane in a calmodulin/IP3 receptor-independent process
    Barbara J Wedel
    Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 278:25758-65. 2003
    ..These findings indicate that the previously identified CIRB region of TRPC3 is involved in its targeting to the plasma membrane by a mechanism that does not involve interaction with IP3 receptors...
  36. pmc TRPC channels function independently of STIM1 and Orai1
    Wayne I Dehaven
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, Department of Health and Human Services, PO Box 12233, Research Triangle Park, NC 27709, USA
    J Physiol 587:2275-98. 2009
    ..This suggests that TRPC signalling and STIM1/Orai1 signalling occur in distinct plasma membrane domains. Thus, TRPC channels appear to be activated by mechanisms dependent on phospholipase C which do not involve the Ca(2+) sensor, STIM1...
  37. ncbi request reprint The regulation of the phosphorylation of inositol 1,3,4-trisphosphate in cell-free preparations and its relevance to the formation of inositol 1,3,4,6-tetrakisphosphate in agonist-stimulated rat parotid acinar cells
    P J Hughes
    Inositol Lipid Section, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709
    J Biol Chem 264:19871-8. 1989
    ..1-0.2 microM), which leads us to propose that inositol 3,4,5,6-tetrakisphosphate is an endogenous inhibitor of the kinase...
  38. ncbi request reprint Canonical transient receptor potential TRPC7 can function as both a receptor- and store-operated channel in HEK-293 cells
    Jean Philippe Lievremont
    National Institute of Environmental Health Sciences, PO Box 12233, Research Triangle Park, NC 27709, USA
    Am J Physiol Cell Physiol 287:C1709-16. 2004
    ..In addition, the results reconcile the apparently conflicting findings of other laboratories regarding TRPC7 regulation...
  39. pmc An inositol 1,4,5-trisphosphate receptor-dependent cation entry pathway in DT40 B lymphocytes
    Guillermo Vazquez
    Calcium Regulation Section, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, PO Box 12233, 111 TW Alexander Drive, Research Triangle Park, NC 27709, USA
    EMBO J 21:4531-8. 2002
    ..These results suggest that in DT40 B lymphocytes, BCR stimulation activates a novel cation entry across the plasma membrane that depends upon, or is mediated by, fully functional IP3R...
  40. ncbi request reprint Expression level of the canonical transient receptor potential 3 (TRPC3) channel determines its mechanism of activation
    Guillermo Vazquez
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health DHHS, Research Triangle Park, NC 27709, USA
    J Biol Chem 278:21649-54. 2003
    ..e. either by phospholipase C-derived messengers or Ca2+ store-depletion. The mechanism of regulation of the channels depends on their level of expression...
  41. ncbi request reprint Differential effects of protein kinase C activation on calcium storage and capacitative calcium entry in NIH 3T3 cells
    C M Ribeiro
    NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 271:21522-8. 1996
    ..These actions indicate a complex and potentially important role for the protein kinase C system in calcium homeostasis in this cell type...
  42. ncbi request reprint Uptake and intracellular sequestration of divalent cations in resting and methacholine-stimulated mouse lacrimal acinar cells. Dissociation by Sr2+ and Ba2+ of agonist-stimulated divalent cation entry from the refilling of the agonist-sensitive intracellu
    C Y Kwan
    Calcium Regulation Section, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709
    J Biol Chem 265:678-84. 1990
    ....
  43. ncbi request reprint "Kissin' cousins": intimate plasma membrane-ER interactions underlie capacitative calcium entry
    J W Putney
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Cell 99:5-8. 1999
  44. ncbi request reprint Signaling mechanism for receptor-activated canonical transient receptor potential 3 (TRPC3) channels
    Mohamed Trebak
    Laboratory of Signal Transduction, NIEHS National Institutes of Health, Research Triangle Park, NC 27709, USA
    J Biol Chem 278:16244-52. 2003
    ..We conclude that receptor-mediated activation of phospholipase C in intact cells activates TRPC3 via diacylglycerol production, independently of G proteins, protein kinase C, or inositol 1,4,5-trisphosphate...
  45. pmc Calcium influx mechanisms underlying calcium oscillations in rat hepatocytes
    Bertina F Jones
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Hepatology 48:1273-81. 2008
    ..Similarly, RNAi knockdown of STIM1 or Orai1 substantially reduced thapsigargin-induced calcium entry, and more modestly diminished the frequency of vasopressin-induced oscillations...
  46. pmc Ca2+-store-dependent and -independent reversal of Stim1 localization and function
    Jeremy T Smyth
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, PO Box 12233, Research Triangle Park, NC 27709, USA
    J Cell Sci 121:762-72. 2008
    ..We conclude that SOCE is tightly coupled to formation of Stim1 puncta, and both SOCE and puncta formation involve a dynamic, reversible signaling complex that probably consists of components in addition to Stim1 and Orai channels...
  47. ncbi request reprint Signalling mechanisms for TRPC3 channels
    James W Putney
    Calcium Regulation Section, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, PO Box 12233, Research Triangle Park, NC 27709, USA
    Novartis Found Symp 258:123-33; discussion 133-9, 155-9, 263-6. 2004
    ..However, in an avian pre-B cell line, TRPC3 can function either as a diacylglycerol-activated channel, or as a SOC. The mechanism of regulation of TRPC3 in this cell line appears to be related to the level of expression of the protein...
  48. ncbi request reprint Mobilization of calcium by inositol trisphosphates from permeabilized rat parotid acinar cells. Evidence for translocation of calcium from uptake to release sites within the inositol 1,4,5-trisphosphate- and thapsigargin-sensitive calcium pool
    F S Menniti
    Calcium Regulation Section, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709
    J Biol Chem 266:13646-53. 1991
    ..We conclude that cell permeabilization reveals a kinetic, and presumably structural, separation of Ca2+ uptake and release sites within the (1,4,5)IP3-regulated intracellular organelle...
  49. ncbi request reprint Stable activation of single Ca2+ release-activated Ca2+ channels in divalent cation-free solutions
    F J Braun
    Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 276:1063-70. 2001
    ..H., and Cahalan, M. D. (1999) Science 283, 836-839), and in RBL-2H3 m1 cells a current with similar properties is activated by store depletion...
  50. pmc Role of the microtubule cytoskeleton in the function of the store-operated Ca2+ channel activator STIM1
    Jeremy T Smyth
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, NIH, Department of Health and Human Services, PO Box 12233, Research Triangle Park, NC 27709, USA
    J Cell Sci 120:3762-71. 2007
    ..We conclude that microtubules play a facilitative role in the SOCE signaling pathway by optimizing the localization of STIM1...
  51. pmc STIM1 is a calcium sensor specialized for digital signaling
    Gary S Bird
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    Curr Biol 19:1724-9. 2009
    ..This arrangement nicely provides a clearly defined and unambiguous signaling system, translating a digital calcium release signal into calcium influx that can signal to downstream effectors...
  52. pmc Cloning and expression of the human transient receptor potential 4 (TRP4) gene: localization and functional expression of human TRP4 and TRP3
    R R McKay
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, PO Box 12233, Research Triangle Park, NC 27709, USA
    Biochem J 351:735-46. 2000
    ....
  53. ncbi request reprint Mutual antagonism of calcium entry by capacitative and arachidonic acid-mediated calcium entry pathways
    D Luo
    Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 276:20186-9. 2001
    ..The findings reveal a novel process of mutual antagonism between two distinct calcium entry pathways. This mutual antagonism may provide an important protective mechanism for the cell, guarding against toxic Ca(2+) overload...
  54. ncbi request reprint Dissociation of regulated trafficking of TRPC3 channels to the plasma membrane from their activation by phospholipase C
    Jeremy T Smyth
    Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 281:11712-20. 2006
    ....
  55. ncbi request reprint Protection of TRPC7 cation channels from calcium inhibition by closely associated SERCA pumps
    Loic Lemonnier
    National Institute of Environmental Health Sciences, NIH, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    FASEB J 20:503-5. 2006
    ..Under physiological conditions, this negative feedback by calcium is attenuated by the presence of closely associated SERCA pumps...
  56. pmc Complex functions of phosphatidylinositol 4,5-bisphosphate in regulation of TRPC5 cation channels
    Mohamed Trebak
    Department of Health and Human Services, Laboratory of Signal Transduction, NIEHS NIH, P O Box 12233, Research Triangle Park, NC 27709, USA
    Pflugers Arch 457:757-69. 2009
    ..These findings indicate complex functions for regulation of TRPC5 by PIP2, and we propose that membrane polyphosphoinositides may have at least two distinct functions in regulating TRPC5 channel activity...
  57. pmc Phosphorylation of STIM1 underlies suppression of store-operated calcium entry during mitosis
    Jeremy T Smyth
    Laboratory of Signal Transduction and National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Nat Cell Biol 11:1465-72. 2009
    ..Therefore, phosphorylation of STIM1 at Ser 486 and Ser 668, and possibly other sites, underlies suppression of SOCE during mitosis...
  58. ncbi request reprint 2-aminoethoxydiphenyl borane activates a novel calcium-permeable cation channel
    Franz Josef Braun
    National Institute of Environmental Health Sciences, P O Box 12233, Research Triangle Park, NC 27709, USA
    Mol Pharmacol 63:1304-11. 2003
    ..This effect of 2-APB further illustrates the complex actions of this compound and reveals the presence in RBL-2H3 m1 cells of a novel, ligand-gated calcium-permeable channel...
  59. ncbi request reprint Calcium signalling in exocrine glands
    G S Bird
    Calcium Regulation Section, Lab Signal Transduction, Nat Inst Environmental Health Sciences, NIH Research Triangle Park, NC 27709, USA
    Eur J Morphol 36:153-6. 1998
    ..The purpose of these studies is to gain understanding into the processes controlling capacitative calcium entry in exocrine gland cells...
  60. pmc Complex regulation of the TRPC3, 6 and 7 channel subfamily by diacylglycerol and phosphatidylinositol-4,5-bisphosphate
    Loic Lemonnier
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences NIH, Research Triangle Park, NC 27709, United States
    Cell Calcium 43:506-14. 2008
    ..These findings reveal complex regulation of TRPC channels by lipid mediators. The results also reveal for the first time direct activation by PIP(2) of members of the TRPC ion channel subfamily...
  61. pmc Capacitative calcium entry supports calcium oscillations in human embryonic kidney cells
    Gary St J Bird
    National Institute of Environmental Health Sciences NIH, PO Box 12233, Research Triangle Park, NC 27709, USA
    J Physiol 562:697-706. 2005
    ..However, by artificial introduction of a non-store-operated pathway into the cells (TRPC3 channels), we find that other Ca(2+) entry mechanisms can influence oscillation frequency in addition to the store-operated channels...
  62. ncbi request reprint Negative regulation of TRPC3 channels by protein kinase C-mediated phosphorylation of serine 712
    Mohamed Trebak
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Mol Pharmacol 67:558-63. 2005
    ..These findings demonstrate a dual role for phospholipase C-generated diacylglycerol, which serves as a signal for TRPC3 activation as well as a signal for negative feedback via protein kinase C-mediated phosphorylation...
  63. pmc Native TRPC7 channel activation by an inositol trisphosphate receptor-dependent mechanism
    Guillermo Vazquez
    NIEHS, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 281:25250-8. 2006
    ..However, with conditions expected to produce greater expression levels, TRPC7 functioned independently of the presence of IP3R. This finding may serve to resolve previously conflicting reports from expression studies of TRPC channels...
  64. pmc Methods for studying store-operated calcium entry
    Gary S Bird
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, P O Box 12233, Research Triangle Park, NC 27709, USA
    Methods 46:204-12. 2008
    ....
  65. ncbi request reprint Emerging perspectives in store-operated Ca2+ entry: roles of Orai, Stim and TRP
    Jeremy T Smyth
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health DHHS, Research Triangle Park, NC 27709, USA
    Biochim Biophys Acta 1763:1147-60. 2006
    ..Here we present molecular, pharmacological, and electrophysiological properties of SOCs, with particular focus on the roles that STIM1 and Orai1 may play in the signaling processes that regulate various pathways of store-operated entry...
  66. ncbi request reprint The TRPC3/6/7 subfamily of cation channels
    Mohamed Trebak
    Laboratory of Signal Transduction, Department of Health and Human Services, National Institute of Environmental Health Sciences, National Institute of Health, P O Box 12233, Research Triangle Park, NC 27709, USA
    Cell Calcium 33:451-61. 2003
    ..This review summarizes the current knowledge about the mechanism of activation of the TRPC3/6/7 subfamily, as well as the potential role of these proteins as components of native Ca2+-permeant channels...
  67. ncbi request reprint Comparison of human TRPC3 channels in receptor-activated and store-operated modes. Differential sensitivity to channel blockers suggests fundamental differences in channel composition
    Mohamed Trebak
    Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 277:21617-23. 2002
    ..The distinct action of these inhibitors on hTRPC3-mediated cation entry in these two cell types may result from their different modes of activation and may also reflect differences in basic channel structure...
  68. ncbi request reprint Obligatory role of Src kinase in the signaling mechanism for TRPC3 cation channels
    Guillermo Vazquez
    Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 279:40521-8. 2004
    ..In both instances, wild-type Src restored TRPC3 regulation. We conclude that Src plays an obligatory role in the mechanism for receptor and diacylglycerol activation of TRPC3...
  69. ncbi request reprint Calcium inhibition and calcium potentiation of Orai1, Orai2, and Orai3 calcium release-activated calcium channels
    Wayne I Dehaven
    Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 282:17548-56. 2007
    ..Orai3 channels appeared to differ from Orai1 and -2 in being somewhat resistant to the process of Ca(2+) depotentiation...
  70. pmc Complex actions of 2-aminoethyldiphenyl borate on store-operated calcium entry
    Wayne I Dehaven
    Laboratory of Signal Transduction, NIEHS, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 283:19265-73. 2008
    ..These data reveal novel and complex actions of 2-APB effects on SOCE that can be attributed to effects on both STIM1 as well as Orai channel subunits...
  71. ncbi request reprint Capacitative calcium entry channels
    J W Putney
    Calcium Regulation Section, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Bioessays 21:38-46. 1999
    ..This review summarizes current knowledge about the nature of capacitative calcium entry signals, as well as the potential role of mammalian TRP proteins as capacitative calcium entry channel molecules...
  72. ncbi request reprint Mechanisms of phospholipase C-regulated calcium entry
    Gary S Bird
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, P O Box 12233, Research Triangle Park, North Carolina 27709, USA
    Curr Mol Med 4:291-301. 2004
    ..This review summarizes current knowledge of the underlying signaling mechanisms and the nature of the channel molecules responsible for these two general categories of regulated Ca2+ entry...
  73. ncbi request reprint Mechanism of inhibition of TRPC cation channels by 2-aminoethoxydiphenylborane
    Jean Philippe Lievremont
    NIEHS, NIH, P O Box 12233, Research Triangle Park, NC 27709, USA
    Mol Pharmacol 68:758-62. 2005
    ..Thus, as concluded for store-operated channels, 2APB seems to inhibit TRPC channels by a direct mechanism not involving IP3 receptors...
  74. ncbi request reprint The mammalian TRPC cation channels
    Guillermo Vazquez
    The Calcium Regulation Section, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, 111 TW Alexander Dr, Research Triangle Park, NC 27709, USA
    Biochim Biophys Acta 1742:21-36. 2004
    ..This review summarizes recent findings on the structure, regulation and function of the apparently ubiquitous TRPC cation channels...
  75. ncbi request reprint PLC-gamma: an old player has a new role
    James W Putney
    Nat Cell Biol 4:E280-1. 2002
  76. ncbi request reprint Store-operated calcium channels
    Anant B Parekh
    Department of Physiology, University of Oxford, United Kingdom
    Physiol Rev 85:757-810. 2005
    ....
  77. ncbi request reprint Ca(2+) mobilization through dorsal root ganglion Ca(2+)-sensing receptor stably expressed in HEK293 cells
    Emmanuel M Awumey
    Cardiovascular Disease Research Program, Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, 700 George St, Durham, NC 27707, USA
    Am J Physiol Cell Physiol 292:C1895-905. 2007
    ..9 +/- 5.2% (at 1 mM Ca(2+)) and 40.5 +/- 6.5% (at 2 mM Ca(2+)), compared with controls. The findings suggest involvement of PKC in the pathway for Ca(2+) mobilization following CaR activation...
  78. pmc STIM1 is a MT-plus-end-tracking protein involved in remodeling of the ER
    Ilya Grigoriev
    Department of Cell Biology, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands
    Curr Biol 18:177-82. 2008
    ..Depletion of STIM1 and EB1 decreases TAC-dependent ER protrusion, indicating that microtubule growth-dependent concentration of STIM1 in the ER membrane plays a role in ER remodeling...
  79. ncbi request reprint Ca2+-calmodulin-dependent facilitation and Ca2+ inactivation of Ca2+ release-activated Ca2+ channels
    Ben Moreau
    Laboratory of Cellular and Molecular Signalling, Department of Physiology, University of Oxford, Parks Road, Oxford OX1 3PT, United Kingdom
    J Biol Chem 280:8776-83. 2005
    ..Hence, the same messenger, intracellular Ca2+, can both facilitate and inactivate Ca2+ entry through store-operated CRAC channels and through different mechanisms...
  80. pmc Defective mast cell effector functions in mice lacking the CRACM1 pore subunit of store-operated calcium release-activated calcium channels
    Monika Vig
    Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Immunol 9:89-96. 2008
    ..Thus, CRACM1 is crucial in mouse mast cell effector function, but mouse T cell calcium release-activated calcium channels are functional in the absence of CRACM1...