Research Topics
| Anu PuriSummaryAffiliation: National Institutes of Health Country: USA Publications
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Detail Information
Publications
Lipid-based nanoparticles as pharmaceutical drug carriers: from concepts to clinicAnu Puri
Center for Cancer Research Nanobiology Program, National Cancer Institute at Frederick, National Institutes of Health, Frederick, MD 21702 1201, USA
Crit Rev Ther Drug Carrier Syst 26:523-80. 2009..We conclude with a few examples of clinically successful formulations of currently available lipid-based nanoparticles...- HER2-specific affibody-conjugated thermosensitive liposomes (Affisomes) for improved delivery of anticancer agentsAnu Puri
CCR Nanobiology Program, NCI Frederick, National Institutes of Health, Frederick, Maryland 21702 1201, USA
J Liposome Res 18:293-307. 2008..Therefore, Affisomes present promising, novel drug-delivery candidates for breast cancer targeting... 
Role of glycosphingolipids in HIV-1 entry: requirement of globotriosylceramide (Gb3) in CD4/CXCR4-dependent fusionA Puri
Section of Membrane Structure and Function, LECB, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Frederick, MD 21702 1201, USA
Biosci Rep 19:317-25. 1999..Therefore, Gb3 functions in conjunction with HIV-1 co-receptor, CXCR4 to promote fusion. We propose that Gb3 functions by recruiting CD4 and/or CXCR4 at the fusion site through structurally specific interactions...- Influenza virus upregulates CXCR4 expression in CD4+ cellsA Puri
Laboratory of Experimental and Computational Biology, NCI FCRDC, Frederick, Maryland 21702, USA
AIDS Res Hum Retroviruses 16:19-25. 2000..Our observations suggest that infectious agents such as influenza may contribute to HIV disease progression by modulating coreceptor availability... - The role of cholesterol and sphingolipids in chemokine receptor function and HIV-1 envelope glycoprotein-mediated fusionSherimay Ablan
Center for Cancer Research Nanobiology Program, Center for Cancer Research, National Cancer Insitute, National Institutes of Health, Frederick, Maryland, USA
Virol J 3:104. 2006..To focus on the role of lipid composition on chemokine receptor function, we have by-passed the CD4 requirement for HIV-1 Env-mediated fusion by using a CD4-independent strain of HIV-1 Env... - Elevated expression of GM3 in receptor-bearing targets confers resistance to human immunodeficiency virus type 1 fusionSatinder S Rawat
Laboratory of Experimental and Computational Biology, Center for Cancer Research NCI-Frederick, NIH, Frederick, MD 21702-1201, USA
J Virol 78:7360-8. 2004..Our findings offer a novel mechanism of interplay between membrane lipids and receptors by which host cells may escape viral infections... - HIV-1 envelope glycoprotein-mediated fusion and pathogenesis: implications for therapy and vaccine developmentAmy Jacobs
Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA
Vaccine 26:3026-35. 2008..Taken as a whole, our studies have many different important implications for antiviral therapies and vaccine development... - Sphingolipids, cholesterol, and HIV-1: a paradigm in viral fusionSatinder Singh Rawat
Laboratory of Experimental and Computational Biology, Center for Cancer Research NCI-Frederick, NIH, P.O. Box B, Bldg. 469, Rm. 211, Miller Drive Frederick, MD, USA
Glycoconj J 23:189-97. 2006..Based on these observations, we propose that the plasma membrane cholesterol is required to maintain the integrity of receptor pools whereas GSLs are involved in stabilizing the coupling of inter-receptor pools... - Restricted lateral mobility of plasma membrane CD4 impairs HIV-1 envelope glycoprotein mediated fusionSatinder S Rawat
CCRNP, NCI Frederick, National Institutes of Health, Frederick, Maryland 21702 1201, USA
Mol Membr Biol 25:83-94. 2008..Our data demonstrate that the lateral mobility of CD4 is an important determinant of HIV-1 fusion/entry... - Sphingolipids: modulators of HIV-1 infection and pathogenesisSatinder S Rawat
Laboratory of Experimental and Computational Biology, Center for Cancer Research, National Cancer Institute at Frederick, National Institutes of Health, P.O. Box B, Bldg. 469, Rm. 211, Miller Drive, Frederick, MD 21702, USA
Biosci Rep 25:329-43. 2005..Recent approaches in the design and development of novel glycosyl derivatives, as anti-HIV agents will be summarized... - Functional expression of CD4, CXCR4, and CCR5 in glycosphingolipid-deficient mouse melanoma GM95 cells and susceptibility to HIV-1 envelope glycoprotein-triggered membrane fusionSatinder S Rawat
Laboratory of Experimental and Computational Biology, Center for Cancer Research, NCI-Frederick, National Institutes of Health, Frederick, MD 21702-1201, USA
Virology 318:55-65. 2004..On the basis of these observations, we propose that target membrane GSLs support HIV-1 Env-mediated fusion at low density of receptors by stabilizing receptor pools in natural targets... - Specific targeting to B cells by lipid-based nanoparticles conjugated with a novel CD22-ScFvKristin Loomis
Center for Cancer Research Nanobiology Program, National Cancer Institute at Frederick, National Institutes of Health, Frederick, MD 21702 1201, USA
Exp Mol Pathol 88:238-49. 2010..Taken together these data suggest that these 2nd-generation liposomes may serve as promising carriers for targeted drug delivery to treat patients suffering from B-cell lymphoma... - Multiple site-specific in vitro labeling of single-chain antibodyBoopathy Ramakrishnan
Center for Cancer Research Nanobiology Program, Center for Cancer Research, NCI Frederick, Frederick, Maryland 21702, USA
Bioconjug Chem 20:1383-9. 2009.... - An inhibitor of glycosphingolipid metabolism blocks HIV-1 infection of primary T-cellsAnu Puri
Laboratory of Experimental and Computational Biology, SAIC-Frederick Inc, NCI Frederick, Frederick, MD 21702, USA
AIDS 18:849-58. 2004..Binding of HIV-1 to CD4 lymphocytes was not affected by PPMP treatment. CONCLUSION: Manipulation of glycosphingolipid metabolic pathways may alter susceptibility of CD4 lymphocytes to HIV-1 entry... - Hyperthermia-triggered intracellular delivery of anticancer agent to HER2(+) cells by HER2-specific affibody (ZHER2-GS-Cys)-conjugated thermosensitive liposomes (HER2(+) affisomes)Brandon Smith
CCR Nanobiology Program, NCI, Frederick, MD, USA
J Control Release 153:187-94. 2011..Therefore, our data demonstrate that HER2(+)affisomes encompass both targeting and triggering potential and hence may prove to be viable nanodrug delivery carriers for breast cancer treatment... - A novel class of photo-triggerable liposomes containing DPPC:DC(8,9)PC as vehicles for delivery of doxorubcin to cellsAmichai Yavlovich
Membrane Structure and Function Section, Nanobiology Program, NCI Frederick, Frederick, MD 21702, USA
Biochim Biophys Acta 1808:117-26. 2011..To our knowledge, this is the first report demonstrating improved cell killing following light-triggered release of an encapsulated anticancer agent from photosensitive liposomes... - Ceramide, a target for antiretroviral therapyCatherine M Finnegan
Laboratories of Experimental and Computational Biology, Molecular Immunoregulation, and Experimental Immunology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA
Proc Natl Acad Sci U S A 101:15452-7. 2004..The minimal toxicity of normal cells exposed to 4-HPR should make the drug exceedingly suitable as an anti-HIV therapeutic... - Light-sensitive lipid-based nanoparticles for drug delivery: design principles and future considerations for biological applicationsAmichai Yavlovich
Center for Cancer Research Nanobiology Program, National Cancer Institute at Frederick, National Institutes of Health, Frederick, Maryland 21702 1201, USA
Mol Membr Biol 27:364-81. 2010..We will conclude with our view point on the future perspectives of light-sensitive liposomes in the clinic... - Glycoside analogs of beta-galactosylceramide, a novel class of small molecule antiviral agents that inhibit HIV-1 entryHimanshu Garg
Membrane Structure and Function Section, Nanobiology Program, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA
Antiviral Res 80:54-61. 2008.... - The HIV Env-mediated fusion reactionStephen A Gallo
Laboratory of Experimental and Computational Biology, Center for Cancer Research, NCI Frederick, National Institute of Health, Miller Drive, Frederick, MD 21702 1201, USA
Biochim Biophys Acta 1614:36-50. 2003..A review of these data that may require an updated version of the original model is presented here... - Modulation of entry of enveloped viruses by cholesterol and sphingolipids (Review)Satinder S Rawat
Laboratory of Experimental and Computational Biology, Center for Cancer Research NCI-Frederick, NIH, PO Box B, Bldg. 469, Rm. 211, Miller Drive Frederick, MD 21702-1201, USA
Mol Membr Biol 20:243-54. 2003..This paper will review literature findings on the contribution of the two raft-associated lipids, cholesterol and sphingolipids in viral entry... - The role of glycosphingolipids in HIV signaling, entry and pathogenesisMathias Viard
Laboratory of Experimental and Computational Biology, Center for Cancer Research, National Cancer Institute-Frederick, National Institutes of Health, Frederick, MD, USA
Glycoconj J 20:213-22. 2004..Finally, we summarize how interactions between HIV and coreceptors leading to signaling and/or fusion can be analyzed by the use of various tyrosine kinase and cytoskeletal inhibitors... - Material properties of matrix lipids determine the conformation and intermolecular reactivity of diacetylenic phosphatidylcholine in the lipid bilayerAnu Puri
Membrane Structure and Function Section, SAIC Frederick, Inc, Nanobiology Program, Center for Cancer Research, Frederick, Maryland 21702, USA
Langmuir 27:15120-8. 2011..These results show that the DC(8,9)PC molecules cluster and assume the preferred conformation in the gel-phase matrix for the UV-triggered polymerization reaction... - Polymeric lipid assemblies as novel theranostic toolsAnu Puri
CCR Nanobiology Program, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA
Acc Chem Res 44:1071-9. 2011.... - Fluorescent lipid probes in the study of viral membrane fusionRobert Blumenthal
Laboratory of Experimental and Computational Biology, Center for Cancer Research, SAIC, P O Box B, Bldg 469, Rm 216A, Miller Drive, NCI Frederick, MD 21702 1201, USA
Chem Phys Lipids 116:39-55. 2002..Thus, fluorescent lipid probes provide a broad repertoire of fusion assays and powerful tools to produce precise, quantitative data in real time required for the elucidation of the complex process of viral fusion... - Quantitative analysis of phospholipids using nanostructured laser desorption ionization targetsSimona Colantonio
Protein Chemistry Laboratory, Advanced Technology Program, SAIC Frederick NCI Frederick, Frederick, MD 21702, USA
Lipids 46:469-77. 2011..The analysis of the lipids before and after UV exposure confirmed a decrease in the signal of DC(8,9)PC of about 90%. In contrast, there was no reduction in DPPC signal... - Lipids in viral fusionAnu Puri
Laboratory of Experimental and Computational Biology, NCI-FCRDC, Frederick, MD, USA
Methods Mol Biol 199:61-81. 2002 - Site specific conjugation of fluoroprobes to the remodeled Fc N-glycans of monoclonal antibodies using mutant glycosyltransferases: application for cell surface antigen detectionElizabeth Boeggeman
Structural Glycobiology Section, CCR Nanobiology Program, SAIC Frederick, Inc, Frederick, Maryland 21702, USA
Bioconjug Chem 20:1228-36. 2009..Our results demonstrate that the linking of cargo molecules to mAbs via glycans could prove to be an invaluable tool for potential drug targeting by immunotherapeutic methods... - HIV-1 gp41 six-helix bundle formation occurs rapidly after the engagement of gp120 by CXCR4 in the HIV-1 Env-mediated fusion processS A Gallo
Laboratory of Experimental and Computational Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA
Biochemistry 40:12231-6. 2001..We show that CXCR4 engagement and six-helix bundle formation only occur after the release of the cytochalasin arrest, indicating that a high degree of cooperativity is required to trigger the initial steps in HIV-1 Env-mediated fusion... - Acid-induced changes in thermal stability and fusion activity of influenza hemagglutininDavid P Remeta
Section on Protein Chemistry, Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, 50 South Drive, Room 2339, Bethesda, Maryland 20892-8012, USA
Biochemistry 41:2044-54. 2002..This finding is consistent with the notion that the fusion activity of influenza virus may involve structural changes of only a small fraction of HA molecules... - Entry of influenza virus into a glycosphingolipid-deficient mouse skin fibroblast cell lineS Ablan
LECB, CCR, NCI-Frederick, National Institutes of Health, Frederick, MD 21702-1201, USA
Arch Virol 146:2227-38. 2001..We conclude that influenza virus uses mainly sialoglycoproteins and that gangliosides are not essential for influenza virus fusion and infection... - P-glycoprotein-overexpressing multidrug-resistant cells are resistant to infection by enveloped viruses that enter via the plasma membraneY Raviv
Intramural Research Support Program SAIC Frederick, Laboratory of Experimental and Computational Biology, National Cancer Institute, Frederick, MD 21702, USA
FASEB J 14:511-5. 2000.... - Interactions of CD4+ plasma membrane vesicles with HIV-1 and HIV-1 envelope glycoprotein-expressing cellsA Puri
Section on Membrane Structure and Function, NCI, NIH, Bethesda, Maryland 20892
J Acquir Immune Defic Syndr 5:915-20. 1992..The CD4 PMVs were more effective in inhibiting syncytia formation than sCD4. These results demonstrate that CD4 PMVs could be used to study the mechanisms of HIV-1 envelope-mediated fusion and have the potential to inactivate HIV-1... - Syntheses and immunomodulatory activity of 3-O-[2'-hydroxy-3'-N,N-disubstituted aminopropan-1'-yl]-alpha-D-glucofuranosesA R Khan
Division of Medicinal Chemistry, Central Drug Research Institute, Lucknow 226001, India
Eur J Med Chem 36:435-45. 2001..All the compounds were tested for their immunomodulatory potential in vitro; seven of them expressed significant immunostimulant activity... - Synthesis and immunostimulant activity of novel analogs of human casein fragment (54-59)R Sahai
Department of Biochemistry, Central Drug Research Institute, Lucknow, India
Immunopharmacol Immunotoxicol 18:511-28. 1996..Significant suppression on the course of Plasmodium berghei infection was also observed on day 4, in the animals treated with hexapeptidse C and D... - Immunomodulatory activity of analog of muramyl dipeptide and their use as adjunct to chemotherapy of Leishmania donovani in hamsterA Puri
Division Biochemistry, Central Drug Research Institute, Lucknow 226001, India
Int Immunopharmacol 5:937-46. 2005..These peptides were found quite effective in both the modes. In adjunct use the treatment may require lower dose of SSG and thereby reduce the chances of drug toxicity...