Anu Puri

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Lipid-based nanoparticles as pharmaceutical drug carriers: from concepts to clinic
    Anu Puri
    Center for Cancer Research Nanobiology Program, National Cancer Institute at Frederick, National Institutes of Health, Frederick, MD 21702 1201, USA
    Crit Rev Ther Drug Carrier Syst 26:523-80. 2009
  2. ncbi request reprint Role of glycosphingolipids in HIV-1 entry: requirement of globotriosylceramide (Gb3) in CD4/CXCR4-dependent fusion
    A Puri
    Section of Membrane Structure and Function, LECB, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Frederick, MD 21702 1201, USA
    Biosci Rep 19:317-25. 1999
  3. ncbi request reprint Influenza virus upregulates CXCR4 expression in CD4+ cells
    A Puri
    Laboratory of Experimental and Computational Biology, NCI FCRDC, Frederick, Maryland 21702, USA
    AIDS Res Hum Retroviruses 16:19-25. 2000
  4. pmc HER2-specific affibody-conjugated thermosensitive liposomes (Affisomes) for improved delivery of anticancer agents
    Anu Puri
    CCR Nanobiology Program, NCI Frederick, National Institutes of Health, Frederick, Maryland 21702 1201, USA
    J Liposome Res 18:293-307. 2008
  5. pmc The role of cholesterol and sphingolipids in chemokine receptor function and HIV-1 envelope glycoprotein-mediated fusion
    Sherimay Ablan
    Center for Cancer Research Nanobiology Program, Center for Cancer Research, National Cancer Insitute, National Institutes of Health, Frederick, Maryland, USA
    Virol J 3:104. 2006
  6. pmc Elevated expression of GM3 in receptor-bearing targets confers resistance to human immunodeficiency virus type 1 fusion
    Satinder S Rawat
    Laboratory of Experimental and Computational Biology, Center for Cancer Research NCI Frederick, NIH, Frederick, MD 21702 1201, USA
    J Virol 78:7360-8. 2004
  7. pmc HIV-1 envelope glycoprotein-mediated fusion and pathogenesis: implications for therapy and vaccine development
    Amy Jacobs
    Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA
    Vaccine 26:3026-35. 2008
  8. ncbi request reprint Sphingolipids, cholesterol, and HIV-1: a paradigm in viral fusion
    Satinder Singh Rawat
    Laboratory of Experimental and Computational Biology, Center for Cancer Research NCI Frederick, NIH, P O Box B, Bldg 469, Rm 211, Miller Drive Frederick, MD, USA
    Glycoconj J 23:189-97. 2006
  9. pmc Restricted lateral mobility of plasma membrane CD4 impairs HIV-1 envelope glycoprotein mediated fusion
    Satinder S Rawat
    CCRNP, NCI Frederick, National Institutes of Health, Frederick, Maryland 21702 1201, USA
    Mol Membr Biol 25:83-94. 2008
  10. ncbi request reprint Sphingolipids: modulators of HIV-1 infection and pathogenesis
    Satinder S Rawat
    Laboratory of Experimental and Computational Biology, Center for Cancer Research, National Cancer Institute at Frederick, National Institutes of Health, P O Box B, Bldg 469, Rm 211, Miller Drive, Frederick, MD 21702, USA
    Biosci Rep 25:329-43. 2005

Collaborators

Detail Information

Publications38

  1. pmc Lipid-based nanoparticles as pharmaceutical drug carriers: from concepts to clinic
    Anu Puri
    Center for Cancer Research Nanobiology Program, National Cancer Institute at Frederick, National Institutes of Health, Frederick, MD 21702 1201, USA
    Crit Rev Ther Drug Carrier Syst 26:523-80. 2009
    ..We conclude with a few examples of clinically successful formulations of currently available lipid-based nanoparticles...
  2. ncbi request reprint Role of glycosphingolipids in HIV-1 entry: requirement of globotriosylceramide (Gb3) in CD4/CXCR4-dependent fusion
    A Puri
    Section of Membrane Structure and Function, LECB, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Frederick, MD 21702 1201, USA
    Biosci Rep 19:317-25. 1999
    ..Therefore, Gb3 functions in conjunction with HIV-1 co-receptor, CXCR4 to promote fusion. We propose that Gb3 functions by recruiting CD4 and/or CXCR4 at the fusion site through structurally specific interactions...
  3. ncbi request reprint Influenza virus upregulates CXCR4 expression in CD4+ cells
    A Puri
    Laboratory of Experimental and Computational Biology, NCI FCRDC, Frederick, Maryland 21702, USA
    AIDS Res Hum Retroviruses 16:19-25. 2000
    ..Our observations suggest that infectious agents such as influenza may contribute to HIV disease progression by modulating coreceptor availability...
  4. pmc HER2-specific affibody-conjugated thermosensitive liposomes (Affisomes) for improved delivery of anticancer agents
    Anu Puri
    CCR Nanobiology Program, NCI Frederick, National Institutes of Health, Frederick, Maryland 21702 1201, USA
    J Liposome Res 18:293-307. 2008
    ..Therefore, Affisomes present promising, novel drug-delivery candidates for breast cancer targeting...
  5. pmc The role of cholesterol and sphingolipids in chemokine receptor function and HIV-1 envelope glycoprotein-mediated fusion
    Sherimay Ablan
    Center for Cancer Research Nanobiology Program, Center for Cancer Research, National Cancer Insitute, National Institutes of Health, Frederick, Maryland, USA
    Virol J 3:104. 2006
    ..To focus on the role of lipid composition on chemokine receptor function, we have by-passed the CD4 requirement for HIV-1 Env-mediated fusion by using a CD4-independent strain of HIV-1 Env...
  6. pmc Elevated expression of GM3 in receptor-bearing targets confers resistance to human immunodeficiency virus type 1 fusion
    Satinder S Rawat
    Laboratory of Experimental and Computational Biology, Center for Cancer Research NCI Frederick, NIH, Frederick, MD 21702 1201, USA
    J Virol 78:7360-8. 2004
    ..Our findings offer a novel mechanism of interplay between membrane lipids and receptors by which host cells may escape viral infections...
  7. pmc HIV-1 envelope glycoprotein-mediated fusion and pathogenesis: implications for therapy and vaccine development
    Amy Jacobs
    Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA
    Vaccine 26:3026-35. 2008
    ..Taken as a whole, our studies have many different important implications for antiviral therapies and vaccine development...
  8. ncbi request reprint Sphingolipids, cholesterol, and HIV-1: a paradigm in viral fusion
    Satinder Singh Rawat
    Laboratory of Experimental and Computational Biology, Center for Cancer Research NCI Frederick, NIH, P O Box B, Bldg 469, Rm 211, Miller Drive Frederick, MD, USA
    Glycoconj J 23:189-97. 2006
    ..Based on these observations, we propose that the plasma membrane cholesterol is required to maintain the integrity of receptor pools whereas GSLs are involved in stabilizing the coupling of inter-receptor pools...
  9. pmc Restricted lateral mobility of plasma membrane CD4 impairs HIV-1 envelope glycoprotein mediated fusion
    Satinder S Rawat
    CCRNP, NCI Frederick, National Institutes of Health, Frederick, Maryland 21702 1201, USA
    Mol Membr Biol 25:83-94. 2008
    ..Our data demonstrate that the lateral mobility of CD4 is an important determinant of HIV-1 fusion/entry...
  10. ncbi request reprint Sphingolipids: modulators of HIV-1 infection and pathogenesis
    Satinder S Rawat
    Laboratory of Experimental and Computational Biology, Center for Cancer Research, National Cancer Institute at Frederick, National Institutes of Health, P O Box B, Bldg 469, Rm 211, Miller Drive, Frederick, MD 21702, USA
    Biosci Rep 25:329-43. 2005
    ..Recent approaches in the design and development of novel glycosyl derivatives, as anti-HIV agents will be summarized...
  11. ncbi request reprint Functional expression of CD4, CXCR4, and CCR5 in glycosphingolipid-deficient mouse melanoma GM95 cells and susceptibility to HIV-1 envelope glycoprotein-triggered membrane fusion
    Satinder S Rawat
    Laboratory of Experimental and Computational Biology, Center for Cancer Research, NCI Frederick, National Institutes of Health, Frederick, MD 21702 1201, USA
    Virology 318:55-65. 2004
    ..On the basis of these observations, we propose that target membrane GSLs support HIV-1 Env-mediated fusion at low density of receptors by stabilizing receptor pools in natural targets...
  12. pmc Specific targeting to B cells by lipid-based nanoparticles conjugated with a novel CD22-ScFv
    Kristin Loomis
    Center for Cancer Research Nanobiology Program, National Cancer Institute at Frederick, National Institutes of Health, Frederick, MD 21702 1201, USA
    Exp Mol Pathol 88:238-49. 2010
    ..Taken together these data suggest that these 2nd-generation liposomes may serve as promising carriers for targeted drug delivery to treat patients suffering from B-cell lymphoma...
  13. ncbi request reprint An inhibitor of glycosphingolipid metabolism blocks HIV-1 infection of primary T-cells
    Anu Puri
    Laboratory of Experimental and Computational Biology, SAIC Frederick Inc, NCI Frederick, Frederick, MD 21702, USA
    AIDS 18:849-58. 2004
    ..However, other target membrane components may also be involved. This study examines the role of glycosphingolipids (GSL) in HIV-1 entry into primary lymphocytes and its modulation by an inhibitor of GSL biosynthesis...
  14. pmc Multiple site-specific in vitro labeling of single-chain antibody
    Boopathy Ramakrishnan
    Center for Cancer Research Nanobiology Program, Center for Cancer Research, NCI Frederick, Frederick, Maryland 21702, USA
    Bioconjug Chem 20:1383-9. 2009
    ....
  15. pmc Hyperthermia-triggered intracellular delivery of anticancer agent to HER2(+) cells by HER2-specific affibody (ZHER2-GS-Cys)-conjugated thermosensitive liposomes (HER2(+) affisomes)
    Brandon Smith
    CCR Nanobiology Program, NCI, Frederick, MD, USA
    J Control Release 153:187-94. 2011
    ..Therefore, our data demonstrate that HER2(+)affisomes encompass both targeting and triggering potential and hence may prove to be viable nanodrug delivery carriers for breast cancer treatment...
  16. pmc A novel class of photo-triggerable liposomes containing DPPC:DC(8,9)PC as vehicles for delivery of doxorubcin to cells
    Amichai Yavlovich
    Membrane Structure and Function Section, Nanobiology Program, NCI Frederick, Frederick, MD 21702, USA
    Biochim Biophys Acta 1808:117-26. 2011
    ..To our knowledge, this is the first report demonstrating improved cell killing following light-triggered release of an encapsulated anticancer agent from photosensitive liposomes...
  17. pmc Light-sensitive lipid-based nanoparticles for drug delivery: design principles and future considerations for biological applications
    Amichai Yavlovich
    Center for Cancer Research Nanobiology Program, National Cancer Institute at Frederick, National Institutes of Health, Frederick, Maryland 21702 1201, USA
    Mol Membr Biol 27:364-81. 2010
    ..We will conclude with our view point on the future perspectives of light-sensitive liposomes in the clinic...
  18. pmc Ceramide, a target for antiretroviral therapy
    Catherine M Finnegan
    Laboratories of Experimental and Computational Biology, Molecular Immunoregulation, and Experimental Immunology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 101:15452-7. 2004
    ..The minimal toxicity of normal cells exposed to 4-HPR should make the drug exceedingly suitable as an anti-HIV therapeutic...
  19. pmc Low-visibility light-intensity laser-triggered release of entrapped calcein from 1,2-bis (tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine liposomes is mediated through a type I photoactivation pathway
    Amichai Yavlovich
    Center for Cancer Research Nanobiology Program, National Cancer Institute, Frederick, MD 21702 1201, USA
    Int J Nanomedicine 8:2575-87. 2013
    ..Taken together, we conclude that low-intensity 514 nm laser-triggered release of Cal-G from photo-triggerable liposomes involves the type I photoreaction pathway...
  20. pmc Mechanism of membrane permeation induced by synthetic β-hairpin peptides
    Kshitij Gupta
    Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland
    Biophys J 105:2093-103. 2013
    ....
  21. ncbi request reprint The role of glycosphingolipids in HIV signaling, entry and pathogenesis
    Mathias Viard
    Laboratory of Experimental and Computational Biology, Center for Cancer Research, National Cancer Institute Frederick, National Institutes of Health, Frederick, MD, USA
    Glycoconj J 20:213-22. 2004
    ..Finally, we summarize how interactions between HIV and coreceptors leading to signaling and/or fusion can be analyzed by the use of various tyrosine kinase and cytoskeletal inhibitors...
  22. ncbi request reprint Modulation of entry of enveloped viruses by cholesterol and sphingolipids (Review)
    Satinder S Rawat
    Laboratory of Experimental and Computational Biology, Center for Cancer Research NCI Frederick, NIH, PO Box B, Bldg 469, Rm 211, Miller Drive Frederick, MD 21702 1201, USA
    Mol Membr Biol 20:243-54. 2003
    ..This paper will review literature findings on the contribution of the two raft-associated lipids, cholesterol and sphingolipids in viral entry...
  23. ncbi request reprint The HIV Env-mediated fusion reaction
    Stephen A Gallo
    Laboratory of Experimental and Computational Biology, Center for Cancer Research, NCI Frederick, National Institute of Health, Miller Drive, Frederick, MD 21702 1201, USA
    Biochim Biophys Acta 1614:36-50. 2003
    ..A review of these data that may require an updated version of the original model is presented here...
  24. pmc Glycoside analogs of beta-galactosylceramide, a novel class of small molecule antiviral agents that inhibit HIV-1 entry
    Himanshu Garg
    Membrane Structure and Function Section, Nanobiology Program, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA
    Antiviral Res 80:54-61. 2008
    ....
  25. pmc Material properties of matrix lipids determine the conformation and intermolecular reactivity of diacetylenic phosphatidylcholine in the lipid bilayer
    Anu Puri
    Membrane Structure and Function Section, SAIC Frederick, Inc, Nanobiology Program, Center for Cancer Research, Frederick, Maryland 21702, USA
    Langmuir 27:15120-8. 2011
    ..These results show that the DC(8,9)PC molecules cluster and assume the preferred conformation in the gel-phase matrix for the UV-triggered polymerization reaction...
  26. pmc Polymeric lipid assemblies as novel theranostic tools
    Anu Puri
    CCR Nanobiology Program, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA
    Acc Chem Res 44:1071-9. 2011
    ....
  27. pmc Quantitative analysis of phospholipids using nanostructured laser desorption ionization targets
    Simona Colantonio
    Protein Chemistry Laboratory, Advanced Technology Program, SAIC Frederick NCI Frederick, Frederick, MD 21702, USA
    Lipids 46:469-77. 2011
    ..The analysis of the lipids before and after UV exposure confirmed a decrease in the signal of DC(8,9)PC of about 90%. In contrast, there was no reduction in DPPC signal...
  28. pmc Site specific conjugation of fluoroprobes to the remodeled Fc N-glycans of monoclonal antibodies using mutant glycosyltransferases: application for cell surface antigen detection
    Elizabeth Boeggeman
    Structural Glycobiology Section, CCR Nanobiology Program, SAIC Frederick, Inc, Frederick, Maryland 21702, USA
    Bioconjug Chem 20:1228-36. 2009
    ..Our results demonstrate that the linking of cargo molecules to mAbs via glycans could prove to be an invaluable tool for potential drug targeting by immunotherapeutic methods...
  29. ncbi request reprint Lipids in viral fusion
    Anu Puri
    Laboratory of Experimental and Computational Biology, NCI FCRDC, Frederick, MD, USA
    Methods Mol Biol 199:61-81. 2002
  30. ncbi request reprint Fluorescent lipid probes in the study of viral membrane fusion
    Robert Blumenthal
    Laboratory of Experimental and Computational Biology, Center for Cancer Research, SAIC, P O Box B, Bldg 469, Rm 216A, Miller Drive, NCI Frederick, MD 21702 1201, USA
    Chem Phys Lipids 116:39-55. 2002
    ..Thus, fluorescent lipid probes provide a broad repertoire of fusion assays and powerful tools to produce precise, quantitative data in real time required for the elucidation of the complex process of viral fusion...
  31. ncbi request reprint HIV-1 gp41 six-helix bundle formation occurs rapidly after the engagement of gp120 by CXCR4 in the HIV-1 Env-mediated fusion process
    S A Gallo
    Laboratory of Experimental and Computational Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA
    Biochemistry 40:12231-6. 2001
    ..We show that CXCR4 engagement and six-helix bundle formation only occur after the release of the cytochalasin arrest, indicating that a high degree of cooperativity is required to trigger the initial steps in HIV-1 Env-mediated fusion...
  32. ncbi request reprint Acid-induced changes in thermal stability and fusion activity of influenza hemagglutinin
    David P Remeta
    Section on Protein Chemistry, Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, 50 South Drive, Room 2339, Bethesda, Maryland 20892 8012, USA
    Biochemistry 41:2044-54. 2002
    ..This finding is consistent with the notion that the fusion activity of influenza virus may involve structural changes of only a small fraction of HA molecules...
  33. ncbi request reprint Entry of influenza virus into a glycosphingolipid-deficient mouse skin fibroblast cell line
    S Ablan
    LECB, CCR, NCI-Frederick, National Institutes of Health, Frederick, MD 21702-1201, USA
    Arch Virol 146:2227-38. 2001
    ..We conclude that influenza virus uses mainly sialoglycoproteins and that gangliosides are not essential for influenza virus fusion and infection...
  34. ncbi request reprint P-glycoprotein-overexpressing multidrug-resistant cells are resistant to infection by enveloped viruses that enter via the plasma membrane
    Y Raviv
    Intramural Research Support Program SAIC Frederick, Laboratory of Experimental and Computational Biology, National Cancer Institute, Frederick, MD 21702, USA
    FASEB J 14:511-5. 2000
    ....
  35. ncbi request reprint Interactions of CD4+ plasma membrane vesicles with HIV-1 and HIV-1 envelope glycoprotein-expressing cells
    A Puri
    Section on Membrane Structure and Function, NCI, NIH, Bethesda, Maryland 20892
    J Acquir Immune Defic Syndr 5:915-20. 1992
    ..The CD4 PMVs were more effective in inhibiting syncytia formation than sCD4. These results demonstrate that CD4 PMVs could be used to study the mechanisms of HIV-1 envelope-mediated fusion and have the potential to inactivate HIV-1...
  36. ncbi request reprint Syntheses and immunomodulatory activity of 3-O-[2'-hydroxy-3'-N,N-disubstituted aminopropan-1'-yl]-alpha-D-glucofuranoses
    A R Khan
    Division of Medicinal Chemistry, Central Drug Research Institute, Lucknow 226001, India
    Eur J Med Chem 36:435-45. 2001
    ..All the compounds were tested for their immunomodulatory potential in vitro; seven of them expressed significant immunostimulant activity...
  37. ncbi request reprint Synthesis and immunostimulant activity of novel analogs of human casein fragment (54-59)
    R Sahai
    Department of Biochemistry, Central Drug Research Institute, Lucknow, India
    Immunopharmacol Immunotoxicol 18:511-28. 1996
    ..Significant suppression on the course of Plasmodium berghei infection was also observed on day 4, in the animals treated with hexapeptidse C and D...
  38. ncbi request reprint Immunomodulatory activity of analog of muramyl dipeptide and their use as adjunct to chemotherapy of Leishmania donovani in hamster
    A Puri
    Division Biochemistry, Central Drug Research Institute, Lucknow 226001, India
    Int Immunopharmacol 5:937-46. 2005
    ..These peptides were found quite effective in both the modes. In adjunct use the treatment may require lower dose of SSG and thereby reduce the chances of drug toxicity...