J Piatigorsky

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Multifunctional lens crystallins and corneal enzymes. More than meets the eye
    J Piatigorsky
    Laboratory of Molecular and Development Biology, National Eye Intitute, National Institutes of Health, Bethesda, Maryland 20892 2730, USA
    Ann N Y Acad Sci 842:7-15. 1998
  2. ncbi request reprint Pax-6 and alphaB-crystallin/small heat shock protein gene regulation in the murine lens. Interaction with the lens-specific regions, LSR1 and LSR2
    R Gopal-Srivastava
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 2730, USA
    J Biol Chem 271:23029-36. 1996
  3. pmc Regulation of the murine alpha B-crystallin/small heat shock protein gene in cardiac muscle
    R Gopal-Srivastava
    Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, Maryland 20892 2730, USA
    Mol Cell Biol 15:7081-90. 1995
  4. ncbi request reprint Pax-6 interactions with TATA-box-binding protein and retinoblastoma protein
    A Cvekl
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 2730, USA
    Invest Ophthalmol Vis Sci 40:1343-50. 1999
  5. pmc Transcriptional regulation of the mouse alpha A-crystallin gene: activation dependent on a cyclic AMP-responsive element (DE1/CRE) and a Pax-6-binding site
    A Cvekl
    Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, Maryland 20892 2730
    Mol Cell Biol 15:653-60. 1995
  6. ncbi request reprint Cloning and characterization of a novel zinc-finger protein-encoding cDNA from the mouse eye lens
    J P Brady
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892
    Gene 124:207-14. 1993
  7. ncbi request reprint Transcriptional regulation of the mouse alpha A-crystallin gene: binding of USF to the -7/+5 region
    C M Sax
    Laboratory of Molecular and Developmental Biology, National Eye Institute, NIH, Bethesda, MD 20892, USA
    Gene 185:209-16. 1997
  8. ncbi request reprint Sequence and expression of chicken beta A2- and beta B3-crystallins
    M K Duncan
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2730, USA
    Exp Eye Res 62:111-9. 1996
  9. ncbi request reprint Protein-DNA interactions of the mouse alpha A-crystallin control regions. Differences between expressing and non-expressing cells
    M Kantorow
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892
    J Mol Biol 230:425-35. 1993
  10. pmc Aldehyde dehydrogenase class 3 expression: identification of a cornea-preferred gene promoter in transgenic mice
    W T Kays
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 94:13594-9. 1997

Collaborators

Detail Information

Publications39

  1. ncbi request reprint Multifunctional lens crystallins and corneal enzymes. More than meets the eye
    J Piatigorsky
    Laboratory of Molecular and Development Biology, National Eye Intitute, National Institutes of Health, Bethesda, Maryland 20892 2730, USA
    Ann N Y Acad Sci 842:7-15. 1998
    ..The use of the same protein for entirely different functions by a gene-sharing mechanism may be a general strategy based on evolutionary tinkering at the level of gene regulation...
  2. ncbi request reprint Pax-6 and alphaB-crystallin/small heat shock protein gene regulation in the murine lens. Interaction with the lens-specific regions, LSR1 and LSR2
    R Gopal-Srivastava
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 2730, USA
    J Biol Chem 271:23029-36. 1996
    ..Our data strengthen the idea that Pax-6 has had a major role in recruiting genes for high expression in the lens...
  3. pmc Regulation of the murine alpha B-crystallin/small heat shock protein gene in cardiac muscle
    R Gopal-Srivastava
    Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, Maryland 20892 2730, USA
    Mol Cell Biol 15:7081-90. 1995
    ..We conclude that the activity of the alpha B-crystallin enhancer in the heart utilizes a reverse CArG box and an E-box-dependent pathway...
  4. ncbi request reprint Pax-6 interactions with TATA-box-binding protein and retinoblastoma protein
    A Cvekl
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 2730, USA
    Invest Ophthalmol Vis Sci 40:1343-50. 1999
    ..To identify proteins that physically interact with Pax-6, a paired domain- and homeodomain (HD)-containing transcription factor that is a key regulator of eye development...
  5. pmc Transcriptional regulation of the mouse alpha A-crystallin gene: activation dependent on a cyclic AMP-responsive element (DE1/CRE) and a Pax-6-binding site
    A Cvekl
    Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, Maryland 20892 2730
    Mol Cell Biol 15:653-60. 1995
    ..These data provide evidence for a synergistic role of Pax-6 and CREB-like proteins for high expression of the mouse alpha A-crystallin gene in the lens...
  6. ncbi request reprint Cloning and characterization of a novel zinc-finger protein-encoding cDNA from the mouse eye lens
    J P Brady
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892
    Gene 124:207-14. 1993
    ..The stably transformed cells did not differ in appearance from untransformed cells, and an analysis of proteins from transformed and untransformed cells failed to detect any differences resulting from over-expression of the pMLZ-4 mRNA...
  7. ncbi request reprint Transcriptional regulation of the mouse alpha A-crystallin gene: binding of USF to the -7/+5 region
    C M Sax
    Laboratory of Molecular and Developmental Biology, National Eye Institute, NIH, Bethesda, MD 20892, USA
    Gene 185:209-16. 1997
    ....
  8. ncbi request reprint Sequence and expression of chicken beta A2- and beta B3-crystallins
    M K Duncan
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2730, USA
    Exp Eye Res 62:111-9. 1996
    ....
  9. ncbi request reprint Protein-DNA interactions of the mouse alpha A-crystallin control regions. Differences between expressing and non-expressing cells
    M Kantorow
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892
    J Mol Biol 230:425-35. 1993
    ..These data indicate that specific differences in protein-DNA interactions with putative control regions are associated with tissue-preferred expression of the mouse alpha A-crystallin gene...
  10. pmc Aldehyde dehydrogenase class 3 expression: identification of a cornea-preferred gene promoter in transgenic mice
    W T Kays
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 94:13594-9. 1997
    ..We demonstrate regulatory sequences capable of directing cornea-specific gene expression, affording the opportunity for genetic engineering in this transparent tissue...
  11. ncbi request reprint Identification of negative-acting and protein-binding elements in the mouse alpha A-crystallin -1556/-1165 region
    C M Sax
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892
    Gene 144:163-9. 1994
    ..We demonstrate that the chicken delta 1-cry intron repressor binds similar nuclear proteins in chicken embryonic lens cells and mouse alpha TN4-1 lens cells.(ABSTRACT TRUNCATED AT 250 WORDS)..
  12. ncbi request reprint Transketolase gene expression in the cornea is influenced by environmental factors and developmentally controlled events
    C M Sax
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland, 20892 2730, USA
    Cornea 19:833-41. 2000
    ..A marked up-regulation of TKT gene expression coincides with the time of eyelid opening in the mouse. Here, we examined whether exposure to incident light contributes to the up-regulation of TKT gene expression during cornea maturation...
  13. ncbi request reprint Developmental regulation of the chicken beta B1-crystallin promoter in transgenic mice
    M K Duncan
    Laboratory of Molecular and Developmental Biology, National Eye Institute National Institutes of Health, Bethesda, MD 20892, USA
    Mech Dev 57:79-89. 1996
    ..5 dpc transgenic mouse embryos. These data show a strict conservation of the lens-specific spatial and temporal regulation of the chicken and mouse beta B1-crystallin genes...
  14. ncbi request reprint Spatial and temporal activity of the alpha B-crystallin/small heat shock protein gene promoter in transgenic mice
    J I Haynes
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Dev Dyn 207:75-88. 1996
    ....
  15. ncbi request reprint A novel cDNA isolated from the mouse eye lens encoding a protein with zinc fingers and a KRAB domain
    J P Brady
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 2730, USA
    DNA Seq 5:389-92. 1995
    ..PCR analysis reveals the presence of pMLZ-8 mRNA in lens, liver, kidney, spleen, and brain of newborn mice...
  16. ncbi request reprint Lens development and crystallin gene expression: many roles for Pax-6
    A Cvekl
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2730, USA
    Bioessays 18:621-30. 1996
    ....
  17. ncbi request reprint Chicken homeobox gene Prox 1 related to Drosophila prospero is expressed in the developing lens and retina
    S I Tomarev
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 2730, USA
    Dev Dyn 206:354-67. 1996
    ..The conserved pattern of Prox 1/prospero gene expression in vertebrates and Drosophila suggests that Prox 1, like Pax-6, may be essential for eye development in different systematic groups...
  18. ncbi request reprint Chicken beta B1 crystallin: gene sequence and evidence for functional conservation of promoter activity between chicken and mouse
    M K Duncan
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2730
    Biochim Biophys Acta 1261:68-76. 1995
    ..These data indicate that the structure and lens-specific expression of this gene are highly conserved although, like other crystallin genes, the 5' flanking sequences have diverged appreciably during evolution...
  19. ncbi request reprint Gene sharing in lens and cornea: facts and implications
    J Piatigorsky
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892-2730, USA
    Prog Retin Eye Res 17:145-74. 1998
    ....
  20. ncbi request reprint The mouse transketolase (TKT) gene: cloning, characterization, and functional promoter analysis
    C Salamon
    Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, Maryland 20892 2730, USA
    Genomics 48:209-20. 1998
    ..Taken together, these observations suggest that oxidative stress may play a role in the regulation of this gene in the cornea...
  21. ncbi request reprint A mouse cDNA encoding a protein with zinc-fingers and a KRAB domain shows similarity to human profilaggrin
    J P Brady
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892
    Gene 149:299-304. 1994
    ..Sequences that hybridize to this cDNA are detectable in ten other mammalian species...
  22. ncbi request reprint Enigma of the abundant water-soluble cytoplasmic proteins of the cornea: the "refracton" hypothesis
    J Piatigorsky
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-2730, USA
    Cornea 20:853-8. 2001
    ..Consequently, it is proposed that the cornea and lens be considered as a single refractive unit, called here the "refracton," to emphasize their similarities and common function...
  23. pmc Preferential transcription of rabbit Aldh1a1 in the cornea: implication of hypoxia-related pathways
    R B Hough
    Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, Maryland 20892, USA
    Mol Cell Biol 24:1324-40. 2004
    ..These experiments suggest that XREs, E-boxes, and PAS domain/basic helix-loop-helix transcription factors (bHLH-PAS) contribute to preferential rabbit Aldh1a1 promoter activity in the cornea, implicating hypoxia-related pathways...
  24. ncbi request reprint Human alpha B-crystallin gene and preferential promoter function in lens
    R A Dubin
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892
    Genomics 7:594-601. 1990
    ....
  25. ncbi request reprint Murine transcription factor alpha A-crystallin binding protein I. Complete sequence, gene structure, expression, and functional inhibition via antisense RNA
    J P Brady
    Laboratory of Molecular and Developmental Biology, NEI, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 270:1221-9. 1995
    ..When introduced into the alpha TN4-1 mouse lens cell line, the antisense construct significantly inhibited expression from a heterologous promoter that utilized the alpha A-CRYBP1 binding site as an enhancer...
  26. pmc Identification of a lens-specific regulatory region (LSR) of the murine alpha B-crystallin gene
    R Gopal-Srivastava
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892
    Nucleic Acids Res 22:1281-6. 1994
    ....
  27. ncbi request reprint Modulation of myocilin/TIGR expression in human trabecular meshwork
    E R Tamm
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland, USA
    Invest Ophthalmol Vis Sci 40:2577-82. 1999
    ..To study factors that modulate myocilin/trabecular meshwork inducible glucocorticoid response protein (TIGR) mRNA expression in human trabecular meshwork (TM)...
  28. ncbi request reprint Transketolase is a major protein in the mouse cornea
    C M Sax
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, MSC 2730, Bethesda, Maryland 20892 2730, USA
    J Biol Chem 271:33568-74. 1996
    ....
  29. pmc Adaptive evolution of small heat shock protein/alpha B-crystallin promoter activity of the blind subterranean mole rat, Spalax ehrenbergi
    R B Hough
    Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:8145-50. 2002
    ..We speculate that selective pressures on metabolic economy may have contributed to these tissue-specific modifications of promoter/enhancer function during adaptation to life underground...
  30. ncbi request reprint Dual use of the transcriptional repressor (CtBP2)/ribbon synapse (RIBEYE) gene: how prevalent are multifunctional genes?
    J Piatigorsky
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Trends Neurosci 24:555-7. 2001
    ..Recently, an approximately 120 kDa protein called RIBEYE has been identified in purified ribbons of bovine retina. The RIBEYE cDNA was cloned and its gene identified in the database...
  31. ncbi request reprint Review: A case for corneal crystallins
    J Piatigorsky
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 2730, USA
    J Ocul Pharmacol Ther 16:173-80. 2000
    ..The use of abundant, taxon-specific water-soluble proteins as crystallins for cellular transparency in the cornea would provide a new conceptual link between this tissue and the lens...
  32. ncbi request reprint The chicken beta A4- and beta B1-crystallin-encoding genes are tightly linked
    M K Duncan
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Gene 162:189-96. 1995
    ....
  33. ncbi request reprint cDNA encoding a chicken protein (CRP1) with homology to hnRNP type A/B
    A Cvekl
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2730, USA
    Biochim Biophys Acta 1261:290-2. 1995
    ..However, the N-terminal 72 amino acids of this deduced chicken protein show no relation to the N-terminal sequences of the other proteins. We call this protein chicken ribonucleoprotein, CRP1...
  34. ncbi request reprint Gene conversion and splice-site slippage in the argininosuccinate lyases/delta-crystallins of the duck lens: members of an enzyme superfamily
    G J Wistow
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892
    Gene 96:263-70. 1990
    ..There may be some clues to the tertiary structures of these conserved motifs in otherwise unrelated proteins for which three-dimensional structures are known...
  35. ncbi request reprint O-Crystallin, arginine kinase and ferritin from the octopus lens
    R D Zinovieva
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, NIH, Bldg 6, Room 2A04, 6 Center Dr MSC 2730, Bethesda, MD 20892 2730, USA
    Biochim Biophys Acta 1431:512-7. 1999
    ..Finally, ferritin cDNA was isolated as an abundant cDNA from the octopus lens library. Northern blots showed that ferritin mRNA is not lens-specific...
  36. ncbi request reprint Lens protein gene expression: alpha-crystallins and MIP
    A B Chepelinsky
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892
    Lens Eye Toxic Res 8:319-44. 1991
    ..The multiplicity of cis-regulatory elements controlling the transcription of these three genes indicates the complexity of the mechanisms that regulate gene expression in the lens...
  37. ncbi request reprint Lens crystallins of invertebrates--diversity and recruitment from detoxification enzymes and novel proteins
    S I Tomarev
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2730, USA
    Eur J Biochem 235:449-65. 1996
    ....
  38. pmc J3-crystallin of the jellyfish lens: similarity to saposins
    J Piatigorsky
    Laboratory of Molecular and Developmental Biology, National Eye Institute, and Center for Molecular Modeling, Center for Information Technology, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 98:12362-7. 2001
    ....
  39. ncbi request reprint Structure and lens expression of the gene encoding chicken beta A3/A1-crystallin
    J B McDermott
    Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892
    Gene 117:193-200. 1992
    ..abstract truncated at 250 words)..