Research Topics
Genomes and Genes
| J PiatigorskySummaryAffiliation: National Institutes of Health Country: USA Publications
| Collaborators
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Detail Information
Publications
Multifunctional lens crystallins and corneal enzymes. More than meets the eyeJ Piatigorsky
Laboratory of Molecular and Development Biology, National Eye Intitute, National Institutes of Health, Bethesda, Maryland 20892 2730, USA
Ann N Y Acad Sci 842:7-15. 1998..The use of the same protein for entirely different functions by a gene-sharing mechanism may be a general strategy based on evolutionary tinkering at the level of gene regulation...
Pax-6 and alphaB-crystallin/small heat shock protein gene regulation in the murine lens. Interaction with the lens-specific regions, LSR1 and LSR2R Gopal-Srivastava
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 2730, USA
J Biol Chem 271:23029-36. 1996..Our data strengthen the idea that Pax-6 has had a major role in recruiting genes for high expression in the lens...
Regulation of the murine alpha B-crystallin/small heat shock protein gene in cardiac muscleR Gopal-Srivastava
Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, Maryland 20892 2730, USA
Mol Cell Biol 15:7081-90. 1995..We conclude that the activity of the alpha B-crystallin enhancer in the heart utilizes a reverse CArG box and an E-box-dependent pathway...
Pax-6 interactions with TATA-box-binding protein and retinoblastoma proteinA Cvekl
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 2730, USA
Invest Ophthalmol Vis Sci 40:1343-50. 1999..To identify proteins that physically interact with Pax-6, a paired domain- and homeodomain (HD)-containing transcription factor that is a key regulator of eye development...
Transcriptional regulation of the mouse alpha A-crystallin gene: activation dependent on a cyclic AMP-responsive element (DE1/CRE) and a Pax-6-binding siteA Cvekl
Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, Maryland 20892 2730
Mol Cell Biol 15:653-60. 1995..These data provide evidence for a synergistic role of Pax-6 and CREB-like proteins for high expression of the mouse alpha A-crystallin gene in the lens...
Cloning and characterization of a novel zinc-finger protein-encoding cDNA from the mouse eye lensJ P Brady
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892
Gene 124:207-14. 1993..The stably transformed cells did not differ in appearance from untransformed cells, and an analysis of proteins from transformed and untransformed cells failed to detect any differences resulting from over-expression of the pMLZ-4 mRNA...
Transcriptional regulation of the mouse alpha A-crystallin gene: binding of USF to the -7/+5 regionC M Sax
Laboratory of Molecular and Developmental Biology, National Eye Institute, NIH, Bethesda, MD 20892, USA
Gene 185:209-16. 1997....
Sequence and expression of chicken beta A2- and beta B3-crystallinsM K Duncan
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2730, USA
Exp Eye Res 62:111-9. 1996....
Protein-DNA interactions of the mouse alpha A-crystallin control regions. Differences between expressing and non-expressing cellsM Kantorow
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892
J Mol Biol 230:425-35. 1993..These data indicate that specific differences in protein-DNA interactions with putative control regions are associated with tissue-preferred expression of the mouse alpha A-crystallin gene...
Aldehyde dehydrogenase class 3 expression: identification of a cornea-preferred gene promoter in transgenic miceW T Kays
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 94:13594-9. 1997..We demonstrate regulatory sequences capable of directing cornea-specific gene expression, affording the opportunity for genetic engineering in this transparent tissue...
Identification of negative-acting and protein-binding elements in the mouse alpha A-crystallin -1556/-1165 regionC M Sax
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892
Gene 144:163-9. 1994..We demonstrate that the chicken delta 1-cry intron repressor binds similar nuclear proteins in chicken embryonic lens cells and mouse alpha TN4-1 lens cells.(ABSTRACT TRUNCATED AT 250 WORDS)..
Developmental regulation of the chicken beta B1-crystallin promoter in transgenic miceM K Duncan
Laboratory of Molecular and Developmental Biology, National Eye Institute National Institutes of Health, Bethesda, MD 20892, USA
Mech Dev 57:79-89. 1996..5 dpc transgenic mouse embryos. These data show a strict conservation of the lens-specific spatial and temporal regulation of the chicken and mouse beta B1-crystallin genes...
Spatial and temporal activity of the alpha B-crystallin/small heat shock protein gene promoter in transgenic miceJ I Haynes
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Dev Dyn 207:75-88. 1996....
A novel cDNA isolated from the mouse eye lens encoding a protein with zinc fingers and a KRAB domainJ P Brady
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 2730, USA
DNA Seq 5:389-92. 1995..PCR analysis reveals the presence of pMLZ-8 mRNA in lens, liver, kidney, spleen, and brain of newborn mice...
Lens development and crystallin gene expression: many roles for Pax-6A Cvekl
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2730, USA
Bioessays 18:621-30. 1996....
Chicken homeobox gene Prox 1 related to Drosophila prospero is expressed in the developing lens and retinaS I Tomarev
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 2730, USA
Dev Dyn 206:354-67. 1996..The conserved pattern of Prox 1/prospero gene expression in vertebrates and Drosophila suggests that Prox 1, like Pax-6, may be essential for eye development in different systematic groups...
Chicken beta B1 crystallin: gene sequence and evidence for functional conservation of promoter activity between chicken and mouseM K Duncan
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2730
Biochim Biophys Acta 1261:68-76. 1995..These data indicate that the structure and lens-specific expression of this gene are highly conserved although, like other crystallin genes, the 5' flanking sequences have diverged appreciably during evolution...
The mouse transketolase (TKT) gene: cloning, characterization, and functional promoter analysisC Salamon
Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, Maryland 20892 2730, USA
Genomics 48:209-20. 1998..Taken together, these observations suggest that oxidative stress may play a role in the regulation of this gene in the cornea...
A mouse cDNA encoding a protein with zinc-fingers and a KRAB domain shows similarity to human profilaggrinJ P Brady
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892
Gene 149:299-304. 1994..Sequences that hybridize to this cDNA are detectable in ten other mammalian species...
Gene sharing in lens and cornea: facts and implicationsJ Piatigorsky
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892-2730, USA
Prog Retin Eye Res 17:145-74. 1998....
Enigma of the abundant water-soluble cytoplasmic proteins of the cornea: the "refracton" hypothesisJ Piatigorsky
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-2730, USA
Cornea 20:853-8. 2001..Consequently, it is proposed that the cornea and lens be considered as a single refractive unit, called here the "refracton," to emphasize their similarities and common function...
Preferential transcription of rabbit Aldh1a1 in the cornea: implication of hypoxia-related pathwaysR B Hough
Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, Maryland 20892, USA
Mol Cell Biol 24:1324-40. 2004..These experiments suggest that XREs, E-boxes, and PAS domain/basic helix-loop-helix transcription factors (bHLH-PAS) contribute to preferential rabbit Aldh1a1 promoter activity in the cornea, implicating hypoxia-related pathways...
Human alpha B-crystallin gene and preferential promoter function in lensR A Dubin
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892
Genomics 7:594-601. 1990....
Murine transcription factor alpha A-crystallin binding protein I. Complete sequence, gene structure, expression, and functional inhibition via antisense RNAJ P Brady
Laboratory of Molecular and Developmental Biology, NEI, National Institutes of Health, Bethesda, Maryland 20892
J Biol Chem 270:1221-9. 1995..When introduced into the alpha TN4-1 mouse lens cell line, the antisense construct significantly inhibited expression from a heterologous promoter that utilized the alpha A-CRYBP1 binding site as an enhancer...
Identification of a lens-specific regulatory region (LSR) of the murine alpha B-crystallin geneR Gopal-Srivastava
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892
Nucleic Acids Res 22:1281-6. 1994....
Modulation of myocilin/TIGR expression in human trabecular meshworkE R Tamm
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland, USA
Invest Ophthalmol Vis Sci 40:2577-82. 1999..To study factors that modulate myocilin/trabecular meshwork inducible glucocorticoid response protein (TIGR) mRNA expression in human trabecular meshwork (TM)...
Transketolase is a major protein in the mouse corneaC M Sax
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, MSC 2730, Bethesda, Maryland 20892 2730, USA
J Biol Chem 271:33568-74. 1996....
The chicken beta A4- and beta B1-crystallin-encoding genes are tightly linkedM K Duncan
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
Gene 162:189-96. 1995....
Adaptive evolution of small heat shock protein/alpha B-crystallin promoter activity of the blind subterranean mole rat, Spalax ehrenbergiR B Hough
Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 99:8145-50. 2002..We speculate that selective pressures on metabolic economy may have contributed to these tissue-specific modifications of promoter/enhancer function during adaptation to life underground...
Dual use of the transcriptional repressor (CtBP2)/ribbon synapse (RIBEYE) gene: how prevalent are multifunctional genes?J Piatigorsky
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
Trends Neurosci 24:555-7. 2001..Recently, an approximately 120 kDa protein called RIBEYE has been identified in purified ribbons of bovine retina. The RIBEYE cDNA was cloned and its gene identified in the database...
Review: A case for corneal crystallinsJ Piatigorsky
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 2730, USA
J Ocul Pharmacol Ther 16:173-80. 2000..The use of abundant, taxon-specific water-soluble proteins as crystallins for cellular transparency in the cornea would provide a new conceptual link between this tissue and the lens...
cDNA encoding a chicken protein (CRP1) with homology to hnRNP type A/BA Cvekl
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2730, USA
Biochim Biophys Acta 1261:290-2. 1995..However, the N-terminal 72 amino acids of this deduced chicken protein show no relation to the N-terminal sequences of the other proteins. We call this protein chicken ribonucleoprotein, CRP1...
Lens crystallins of invertebrates--diversity and recruitment from detoxification enzymes and novel proteinsS I Tomarev
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 2730, USA
Eur J Biochem 235:449-65. 1996....
Lens protein gene expression: alpha-crystallins and MIPA B Chepelinsky
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892
Lens Eye Toxic Res 8:319-44. 1991..The multiplicity of cis-regulatory elements controlling the transcription of these three genes indicates the complexity of the mechanisms that regulate gene expression in the lens...
O-Crystallin, arginine kinase and ferritin from the octopus lensR D Zinovieva
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, NIH, Bldg 6, Room 2A04, 6 Center Dr MSC 2730, Bethesda, MD 20892 2730, USA
Biochim Biophys Acta 1431:512-7. 1999..Finally, ferritin cDNA was isolated as an abundant cDNA from the octopus lens library. Northern blots showed that ferritin mRNA is not lens-specific...
J3-crystallin of the jellyfish lens: similarity to saposinsJ Piatigorsky
Laboratory of Molecular and Developmental Biology, National Eye Institute, and Center for Molecular Modeling, Center for Information Technology, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 98:12362-7. 2001....
Structure and lens expression of the gene encoding chicken beta A3/A1-crystallinJ B McDermott
Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892
Gene 117:193-200. 1992..abstract truncated at 250 words)..
