Caroline C Philpott

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Metabolic remodeling in iron-deficient fungi
    Caroline C Philpott
    Genetics and Metabolism Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bldg 10, Rm 9B 16, 10 Center Drive, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1823:1509-20. 2012
  2. pmc Coming into view: eukaryotic iron chaperones and intracellular iron delivery
    Caroline C Philpott
    Liver Diseases Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 287:13518-23. 2012
  3. pmc Cell-cycle arrest and inhibition of G1 cyclin translation by iron in AFT1-1(up) yeast
    C C Philpott
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 5430, USA
    EMBO J 17:5026-36. 1998
  4. ncbi request reprint Molecular aspects of iron absorption: Insights into the role of HFE in hemochromatosis
    Caroline C Philpott
    Liver Diseases Section, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 1800, USA
    Hepatology 35:993-1001. 2002
  5. ncbi request reprint The response to iron deprivation in Saccharomyces cerevisiae: expression of siderophore-based systems of iron uptake
    C C Philpott
    Liver Diseases Section, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Building 10, Room 9B 16, 10 Center Drive, Bethesda, MD 20892 1800, USA
    Biochem Soc Trans 30:698-702. 2002
  6. pmc Response to iron deprivation in Saccharomyces cerevisiae
    Caroline C Philpott
    Liver Diseases Branch, National Institutes of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Building 10, Room 9B 16, 10 Center Drive, MSC 1800, Bethesda, MD 20892 1800, USA
    Eukaryot Cell 7:20-7. 2008
  7. ncbi request reprint Iron uptake in fungi: a system for every source
    Caroline C Philpott
    Liver Disease Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    Biochim Biophys Acta 1763:636-45. 2006
  8. pmc Phosphatidylserine is involved in the ferrichrome-induced plasma membrane trafficking of Arn1 in Saccharomyces cerevisiae
    Yan Guo
    Liver Diseases Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 1800, USA
    J Biol Chem 285:39564-73. 2010
  9. pmc Each member of the poly-r(C)-binding protein 1 (PCBP) family exhibits iron chaperone activity toward ferritin
    Sébastien Leidgens
    From the Liver Diseases Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 1800, USA
    J Biol Chem 288:17791-802. 2013
  10. pmc Gga2 mediates sequential ubiquitin-independent and ubiquitin-dependent steps in the trafficking of ARN1 from the trans-Golgi network to the vacuole
    Yi Deng
    Liver Diseases Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 284:23830-41. 2009

Collaborators

  • Yan Guo
  • William A Prinz
  • Patrick O Brown
  • Alvin Berger
  • Martin Bard
  • David Botstein
  • Juan S Bonifacino
  • Jerry Kaplan
  • Simon Labbe
  • Richard K Bruick
  • Minoo Shakoury-Elizeh
  • Olga Protchenko
  • Youngwoo Kim
  • Anjali Nandal
  • Timothy L Stemmler
  • Haifeng Shi
  • Yi Deng
  • Sébastien Leidgens
  • Poorna Subramanian
  • Rachel Androphy
  • Yuriy R Boretsky
  • Benoit Pelletier
  • Robert E Moore
  • Kimberly Z Bullough
  • Fengmin Li
  • Emory Hsu
  • Navin Natarajan
  • Toshiki Yabe
  • Julio C Ruiz
  • Sudipa Ghimire-Rijal
  • Ruth Ann Sinnamon
  • Teresa M Dunn
  • Kenneth Gable
  • James Cox
  • Munira A Basrai
  • Wei Chun Au
  • Hadiya Watson
  • Joshua Storey
  • Patricia Keane
  • Krisztina Z Bencze
  • Yuriy V Pynyaha
  • Olga V Protchenko
  • Vasyl I Kutsyaba
  • Andriy A Sibirny
  • Volodymyr Y Boretsky
  • Roberto Rodriguez-Suarez
  • Howard Bussey
  • Sarah M Lampert
  • Emily Garcia
  • John Tiedeman
  • Tracey Ferea
  • Ronda Rolfes
  • Janos Demeter
  • Jared Rashford
  • Jude Beaudoin
  • Cheol Won Yun

Detail Information

Publications24

  1. pmc Metabolic remodeling in iron-deficient fungi
    Caroline C Philpott
    Genetics and Metabolism Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bldg 10, Rm 9B 16, 10 Center Drive, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1823:1509-20. 2012
    ..However, the regulatory mechanisms that accomplish these adaptations differ greatly between fungal species. This article is part of a Special Issue entitled: Cell Biology of Metals...
  2. pmc Coming into view: eukaryotic iron chaperones and intracellular iron delivery
    Caroline C Philpott
    Liver Diseases Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 287:13518-23. 2012
    ..Although the molecular details have yet to be explored, these proteins, acting independently or together, may represent the basic cellular machinery for intracellular iron delivery...
  3. pmc Cell-cycle arrest and inhibition of G1 cyclin translation by iron in AFT1-1(up) yeast
    C C Philpott
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 5430, USA
    EMBO J 17:5026-36. 1998
    ..Instead, this reduction occurs at the level of Cln2 translation, a recently recognized locus of cell-cycle control in yeast...
  4. ncbi request reprint Molecular aspects of iron absorption: Insights into the role of HFE in hemochromatosis
    Caroline C Philpott
    Liver Diseases Section, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 1800, USA
    Hepatology 35:993-1001. 2002
    ..This review focuses on the molecular aspects of iron absorption and the role that HFE may play in these processes...
  5. ncbi request reprint The response to iron deprivation in Saccharomyces cerevisiae: expression of siderophore-based systems of iron uptake
    C C Philpott
    Liver Diseases Section, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Building 10, Room 9B 16, 10 Center Drive, Bethesda, MD 20892 1800, USA
    Biochem Soc Trans 30:698-702. 2002
    ..The Arn transporters are expressed in intracellular vesicles that correspond to the endosomal compartment, which suggests that intracellular trafficking of the siderophore and/or its transporter may be important for uptake...
  6. pmc Response to iron deprivation in Saccharomyces cerevisiae
    Caroline C Philpott
    Liver Diseases Branch, National Institutes of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Building 10, Room 9B 16, 10 Center Drive, MSC 1800, Bethesda, MD 20892 1800, USA
    Eukaryot Cell 7:20-7. 2008
  7. ncbi request reprint Iron uptake in fungi: a system for every source
    Caroline C Philpott
    Liver Disease Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    Biochim Biophys Acta 1763:636-45. 2006
    ..This system recognizes a broad range of substrates. The non-reductive system exhibits specificity for siderophore-iron chelates, and transporters of this system exhibit multiple substrate-dependent intracellular trafficking events...
  8. pmc Phosphatidylserine is involved in the ferrichrome-induced plasma membrane trafficking of Arn1 in Saccharomyces cerevisiae
    Yan Guo
    Liver Diseases Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 1800, USA
    J Biol Chem 285:39564-73. 2010
    ..Strains with defects in phospholipid synthesis also exhibited alterations in Arn1p trafficking, indicating that the intracellular trafficking of some transporters is dependent on the phospholipid composition of the cellular membranes...
  9. pmc Each member of the poly-r(C)-binding protein 1 (PCBP) family exhibits iron chaperone activity toward ferritin
    Sébastien Leidgens
    From the Liver Diseases Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 1800, USA
    J Biol Chem 288:17791-802. 2013
    ..Thus, PCBP1 and -2 form a complex for iron delivery to ferritin, and all PCBPs may share iron chaperone activity...
  10. pmc Gga2 mediates sequential ubiquitin-independent and ubiquitin-dependent steps in the trafficking of ARN1 from the trans-Golgi network to the vacuole
    Yi Deng
    Liver Diseases Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 284:23830-41. 2009
    ..These studies suggest that Gga2 is involved in sorting at both the TGN and multivesicular body and that the first step can occur without ubiquitin binding...
  11. pmc GGA2- and ubiquitin-dependent trafficking of Arn1, the ferrichrome transporter of Saccharomyces cerevisiae
    Youngwoo Kim
    Genetics and Metabolism Section, Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 18:1790-802. 2007
    ..Without this ubiquitination, Arn1 remained on the plasma membrane, where it was active for transport. Arn1 was preferentially modified with polyubiquitin chains on a cluster of lysine residues at the amino terminus of the transporter...
  12. pmc Role of PUG1 in inducible porphyrin and heme transport in Saccharomyces cerevisiae
    Olga Protchenko
    Genetics and Metabolism Section, Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bldg 10, Rm 9B 16, Bethesda, MD 20892, USA
    Eukaryot Cell 7:859-71. 2008
    ..The data from these experiments were consistent with a role for Pug1p in inducible protoporphyrin IX influx and heme efflux...
  13. pmc A receptor domain controls the intracellular sorting of the ferrichrome transporter, ARN1
    Youngwoo Kim
    Liver Diseases Branch, National Institutes of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 24:952-62. 2005
    ..We propose that the carboxyl terminus of Arn1p contains a receptor domain that controls the intracellular trafficking of the transporter...
  14. ncbi request reprint A screen for genes of heme uptake identifies the FLC family required for import of FAD into the endoplasmic reticulum
    Olga Protchenko
    Liver Diseases Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 281:21445-57. 2006
    ..We propose that the FLC genes are required for import of FAD into the lumen of the endoplasmic reticulum, where it is required for disulfide bond formation...
  15. pmc Metabolic response to iron deficiency in Saccharomyces cerevisiae
    Minoo Shakoury-Elizeh
    Liver Diseases Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 285:14823-33. 2010
    ..Amino acid homeostasis was robust, but iron deficiency impaired lipid synthesis, altering the properties and functions of cellular membranes...
  16. ncbi request reprint Regulation of intracellular heme levels by HMX1, a homologue of heme oxygenase, in Saccharomyces cerevisiae
    Olga Protchenko
    Liver Diseases Section, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 1800, USA
    J Biol Chem 278:36582-7. 2003
    ..Hmx1p promotes both the reutilization of heme iron and the regulation of heme-dependent transcription during periods of iron scarcity...
  17. pmc A cytosolic iron chaperone that delivers iron to ferritin
    Haifeng Shi
    Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Science 320:1207-10. 2008
    ..Depletion of PCBP1 in human cells inhibited ferritin iron loading and increased cytosolic iron pools. Thus, PCBP1 can function as a cytosolic iron chaperone in the delivery of iron to ferritin...
  18. pmc Transcriptional remodeling in response to iron deprivation in Saccharomyces cerevisiae
    Minoo Shakoury-Elizeh
    Liver Diseases Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Biol Cell 15:1233-43. 2004
    ..We provide evidence that yeast subjected to iron deprivation undergo a transcriptional remodeling, resulting in a shift from iron-dependent to parallel, but iron-independent, metabolic pathways...
  19. pmc Activation of the HIF prolyl hydroxylase by the iron chaperones PCBP1 and PCBP2
    Anjali Nandal
    Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Metab 14:647-57. 2011
    ..These data indicated that PCBP1 was required for iron incorporation into PHD and suggest a broad role for PCBP1 and 2 in delivering iron to cytosolic nonheme iron enzymes...
  20. pmc Ferrichrome induces endosome to plasma membrane cycling of the ferrichrome transporter, Arn1p, in Saccharomyces cerevisiae
    Youngwoo Kim
    Liver Diseases Section, NIDDK, National Institutes of Health, Building 10, Room 9B16, 10 Center Drive, Bethesda, MD 20892 1800, USA
    EMBO J 21:3632-42. 2002
    ..Thus, siderophores influence the trafficking of the Arn transporters within the cell and this trafficking is important for transporter function...
  21. pmc Fep1 represses expression of the fission yeast Schizosaccharomyces pombe siderophore-iron transport system
    Benoit Pelletier
    Departement de Biochimie, Universite de Sherbrooke, Sherbrooke, Quebec J1H 5N4, Canada
    Nucleic Acids Res 31:4332-44. 2003
    ..Taken together, these results demonstrate that Fep1 occupies a central role in coordinating transcriptional regulation of genes encoding components of the reductive and non-reductive iron transport systems in fission yeast...
  22. ncbi request reprint Development of a transformation system for gene knock-out in the flavinogenic yeast Pichia guilliermondii
    Yuriy R Boretsky
    Department of Molecular Genetics and Biotechnology, Institute of Cell Biology NAS of Ukraine, Dragomanov str 14 16, Lviv 79005, Ukraine
    J Microbiol Methods 70:13-9. 2007
    ..Efficient gene deletion in P. guilliermondii can be achieved using the modified URA3 gene of S. cerevisiae flanked by 0.8-0.9 kb sequences homologous to the target gene...
  23. pmc The mechanism of ferrichrome transport through Arn1p and its metabolism in Saccharomyces cerevisiae
    Robert E Moore
    Department of Chemistry, University of Wisconsin, 800 Algoma Boulevard, Oshkosh, WI 54901 8645, USA
    Proc Natl Acad Sci U S A 100:5664-9. 2003
    ..Iron stored as ferrichrome was readily mobilized to meet the metabolic needs of the cell...
  24. ncbi request reprint Iron-dependent metabolic remodeling in S. cerevisiae
    Jerry Kaplan
    Department of Pathology, School of Medicine University of Utah, Salt Lake City, UT 84132 2501, USA
    Biochim Biophys Acta 1763:646-51. 2006
    ..In this review, we describe how the budding yeast Saccharomyces cerevisiae utilizes multiple mechanisms to optimize iron usage under iron limiting conditions...