Stephen P Perfetto

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. doi request reprint Quality assurance for polychromatic flow cytometry using a suite of calibration beads
    Stephen P Perfetto
    Flow Cytometry Core Facility, Vaccine Research Center, National Institute of Allergy and Infectious Diseases NIAID, National Institutes of Health NIH, Bethesda, MD, USA
    Nat Protoc 7:2067-79. 2012
  2. ncbi request reprint Quality assurance for polychromatic flow cytometry
    Stephen P Perfetto
    Flow Cytometry Core Facility, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Protoc 1:1522-30. 2006
  3. pmc Amine-reactive dyes for dead cell discrimination in fixed samples
    Stephen P Perfetto
    Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
    Curr Protoc Cytom . 2010
  4. ncbi request reprint Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry
    Stephen P Perfetto
    Immunology Laboratory, Vaccine Research Center, NIAID, NIH, 40 Convent Dr, Room 5509, Bethesda, MD 20892, USA
    J Immunol Methods 313:199-208. 2006
  5. doi request reprint CCR2 identifies a stable population of human effector memory CD4+ T cells equipped for rapid recall response
    Hongwei H Zhang
    Inflammation Biology Section, Laboratory of Molecular Immunology, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 185:6646-63. 2010
  6. ncbi request reprint Viable infectious cell sorting in a BSL-3 facility
    Stephen P Perfetto
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 263:419-24. 2004
  7. ncbi request reprint Vaccination in humans generates broad T cell cytokine responses
    Stephen C De Rosa
    Vaccine Research Center, National Institute of Allery and Infectious Diseases, NIH, Bethesda, MD 20892, USA
    J Immunol 173:5372-80. 2004
  8. ncbi request reprint Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry
    Pratip K Chattopadhyay
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Med 12:972-7. 2006
  9. pmc Characterization of subsets of CD4+ memory T cells reveals early branched pathways of T cell differentiation in humans
    Kaimei Song
    Inflammation Biology Section, Laboratory of Molecular Immunology and Sections of Human Immunology and ImmunoTechnology, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:7916-21. 2005
  10. doi request reprint Standard practice for cell sorting in a BSL-3 facility
    Stephen P Perfetto
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 699:449-69. 2011

Detail Information

Publications20

  1. doi request reprint Quality assurance for polychromatic flow cytometry using a suite of calibration beads
    Stephen P Perfetto
    Flow Cytometry Core Facility, Vaccine Research Center, National Institute of Allergy and Infectious Diseases NIAID, National Institutes of Health NIH, Bethesda, MD, USA
    Nat Protoc 7:2067-79. 2012
    ..In sum, the procedures presented here represent an updated framework for optimizing, calibrating and standardizing a flow cytometer for daily use...
  2. ncbi request reprint Quality assurance for polychromatic flow cytometry
    Stephen P Perfetto
    Flow Cytometry Core Facility, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Protoc 1:1522-30. 2006
    ..All three aspects of this program should be performed upon installation, or whenever changes occur along the flow cytometer's optical path. However, only a few of these procedures need to be carried out on a routine basis...
  3. pmc Amine-reactive dyes for dead cell discrimination in fixed samples
    Stephen P Perfetto
    Vaccine Research Center, NIAID, NIH, Bethesda, Maryland, USA
    Curr Protoc Cytom . 2010
    ..This unit describes procedures, troubleshooting, and outcomes for using the two most commonly used amine-reactive dyes, ViViD and Aqua Blue...
  4. ncbi request reprint Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry
    Stephen P Perfetto
    Immunology Laboratory, Vaccine Research Center, NIAID, NIH, 40 Convent Dr, Room 5509, Bethesda, MD 20892, USA
    J Immunol Methods 313:199-208. 2006
    ..Amine reactive viability dyes are a powerful tool for fluorescence immunophenotyping experiments...
  5. doi request reprint CCR2 identifies a stable population of human effector memory CD4+ T cells equipped for rapid recall response
    Hongwei H Zhang
    Inflammation Biology Section, Laboratory of Molecular Immunology, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 185:6646-63. 2010
    ....
  6. ncbi request reprint Viable infectious cell sorting in a BSL-3 facility
    Stephen P Perfetto
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 263:419-24. 2004
    ..With this system in place, aerosol containment can be measured quickly and efficiently, therefore reducing the risk to the operator when sorting viable infectious cells...
  7. ncbi request reprint Vaccination in humans generates broad T cell cytokine responses
    Stephen C De Rosa
    Vaccine Research Center, National Institute of Allery and Infectious Diseases, NIH, Bethesda, MD 20892, USA
    J Immunol 173:5372-80. 2004
    ..The presence and variability of these complex response profiles introduce the possibility that selective functional expression patterns may provide correlates for vaccine efficacy or disease progression...
  8. ncbi request reprint Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry
    Pratip K Chattopadhyay
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Med 12:972-7. 2006
    ....
  9. pmc Characterization of subsets of CD4+ memory T cells reveals early branched pathways of T cell differentiation in humans
    Kaimei Song
    Inflammation Biology Section, Laboratory of Molecular Immunology and Sections of Human Immunology and ImmunoTechnology, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:7916-21. 2005
    ..Rather, developmental pathways branch early on to yield effector/memory populations that are highly heterogeneous and multifunctional and have the potential to become stable resting cells...
  10. doi request reprint Standard practice for cell sorting in a BSL-3 facility
    Stephen P Perfetto
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 699:449-69. 2011
    ..Subjects covered include containment verification, remote operation, disinfection, personal protective equipment (PPE), and instrument-specific modifications for enhanced aerosol evacuation...
  11. pmc Heavy metal contaminants can eliminate quantum dot fluorescence
    David Zarkowsky
    Laboratory of Immunology, Vaccine Research Center, NIAID, NIH, Bethesda, Maryland USA
    Cytometry A 79:84-9. 2011
    ..Indeed, these cells can then be successfully stained with other QD reagents, providing a method for immunofluorescence restaining of cells without contaminating fluorescence from the first stain...
  12. ncbi request reprint Seventeen-colour flow cytometry: unravelling the immune system
    Stephen P Perfetto
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Room 5507, Bethesda, Maryland 20892 3015, United States
    Nat Rev Immunol 4:648-55. 2004
  13. doi request reprint The use of quantum dot nanocrystals in multicolor flow cytometry
    Pratip K Chattopadhyay
    Immunotechnology Section, Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA
    Wiley Interdiscip Rev Nanomed Nanobiotechnol 2:334-48. 2010
    ..This article discusses the value of QDs in multicolor flow cytometry, introduces strategies to successfully incorporate QDs into routine use, and highlights emerging applications of the technology...
  14. ncbi request reprint Increased immunofluorescence sensitivity using 532 nm laser excitation
    Stephen P Perfetto
    Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892 3015, USA
    Cytometry A 71:73-9. 2007
    ..We evaluated the use of a high power, diode pulsed solid-state laser emitting 532 nm light for immunofluorescence applications. We compared the sensitivity and utility of this laser with the standard 488 nm excitation...
  15. ncbi request reprint Ontogeny of gamma delta T cells in humans
    Stephen C De Rosa
    Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 172:1637-45. 2004
    ..This represents the earliest immunological maturation of any lymphocyte compartment in humans and most likely indicates the importance of these cells in controlling pathology due to common environmental challenges...
  16. ncbi request reprint Measuring containment of viable infectious cell sorting in high-velocity cell sorters
    Stephen P Perfetto
    Vaccine Research Center, NAID, National Institute of Health, Bethesda, Maryland 20892 3015, USA
    Cytometry A 52:122-30. 2003
    ..With the advent of high-speed sorters, aerosols are a considerable safety concern when sorting viable infectious materials. We describe a four-part safety procedure for validating the containment...
  17. ncbi request reprint Standard safety practices for sorting of unfixed cells
    Ingrid Schmid
    David Geffen School of Medicine at UCLA, Los Angeles, California, USA
    Curr Protoc Cytom . 2007
    ....
  18. ncbi request reprint International Society for Analytical Cytology biosafety standard for sorting of unfixed cells
    Ingrid Schmid
    Department of Hematology Oncology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    Cytometry A 71:414-37. 2007
    ..However, the field of cytometry has progressed since 1997, and the document requires an update...
  19. ncbi request reprint Biohazard sorting
    Ingrid Schmid
    David Geffen School of Medicine, University of California Los Angeles, Department of Hematology Oncology, Los Angeles, California 90095, USA
    Methods Cell Biol 75:221-40. 2004
  20. ncbi request reprint Biosafety concerns for shared flow cytometry core facilities
    Ingrid Schmid
    David Geffen School of Medicine at UCLA, Department of Hematology Oncology, Los Angeles, California 90095, USA
    Cytometry A 56:113-9. 2003
    ..In this report we review safety issues that are pertinent to flow cytometry core facilities by discussing the individual components of this biosafety questionnaire...