Gustavo Pacheco Rodriguez

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint BIG1 and BIG2: brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors
    Gustavo Pacheco-Rodriguez
    Pulmonary Critical Care Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Methods Enzymol 345:397-404. 2002
  2. ncbi request reprint TSC2 loss in lymphangioleiomyomatosis cells correlated with expression of CD44v6, a molecular determinant of metastasis
    Gustavo Pacheco-Rodriguez
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892 1434, USA
    Cancer Res 67:10573-81. 2007
  3. pmc Protein kinase A-anchoring (AKAP) domains in brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2)
    Hewang Li
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 100:1627-32. 2003
  4. pmc Interaction of FK506-binding protein 13 with brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1): effects of FK506
    Philip Ian Padilla
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 10, Room 5N307, MSC 1434, Bethesda, MD 20892 1434, USA
    Proc Natl Acad Sci U S A 100:2322-7. 2003
  5. pmc E3 ubiquitin ligase activity of the trifunctional ARD1 (ADP-ribosylation factor domain protein 1)
    Alessandro Vichi
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1434, USA
    Proc Natl Acad Sci U S A 102:1945-50. 2005
  6. pmc Effect of protein kinase A on accumulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) in HepG2 cell nuclei
    Carmen Citterio
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:2683-8. 2006
  7. pmc Association of brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2) with recycling endosomes during transferrin uptake
    Xiaoyan Shen
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:2635-40. 2006
  8. pmc Nuclear localization and molecular partners of BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors
    Philip Ian Padilla
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 101:2752-7. 2004
  9. pmc Unfolded protein response and cell death after depletion of brefeldin A-inhibited guanine nucleotide-exchange protein GBF1
    Carmen Citterio
    Translational Medicine Branch and Proteomics Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 105:2877-82. 2008
  10. pmc Lymphangioleiomyomatosis (LAM): molecular insights lead to targeted therapies
    Connie G Glasgow
    Translational Medicine Branch, NHLBI, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892 1590, USA
    Respir Med 104:S45-58. 2010

Collaborators

Detail Information

Publications30

  1. ncbi request reprint BIG1 and BIG2: brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors
    Gustavo Pacheco-Rodriguez
    Pulmonary Critical Care Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Methods Enzymol 345:397-404. 2002
  2. ncbi request reprint TSC2 loss in lymphangioleiomyomatosis cells correlated with expression of CD44v6, a molecular determinant of metastasis
    Gustavo Pacheco-Rodriguez
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892 1434, USA
    Cancer Res 67:10573-81. 2007
    ..00003) and may be a biomarker for LAM. The cell surface receptor CD44 and its splice variant CD44v6 may contribute to the metastatic potential of LAM cells...
  3. pmc Protein kinase A-anchoring (AKAP) domains in brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2)
    Hewang Li
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 100:1627-32. 2003
    ..All findings are consistent with a role for BIG2 as an A kinase-anchoring protein (or AKAP) that could coordinate cAMP and ARF regulatory pathways...
  4. pmc Interaction of FK506-binding protein 13 with brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1): effects of FK506
    Philip Ian Padilla
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 10, Room 5N307, MSC 1434, Bethesda, MD 20892 1434, USA
    Proc Natl Acad Sci U S A 100:2322-7. 2003
    ..These findings are consistent with a role for FKBP13 and FK506 in vesicular trafficking, influencing ARF activity through their guanine nucleotide-exchange proteins...
  5. pmc E3 ubiquitin ligase activity of the trifunctional ARD1 (ADP-ribosylation factor domain protein 1)
    Alessandro Vichi
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1434, USA
    Proc Natl Acad Sci U S A 102:1945-50. 2005
    ..Deletion of the RING region or point mutations within the RING sequence abolished ARD1 E3 ligase activity. All data are consistent with a potential function for ARD1 as an E3 ubiquitin ligase in cells...
  6. pmc Effect of protein kinase A on accumulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) in HepG2 cell nuclei
    Carmen Citterio
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:2683-8. 2006
    ..Thus, two more critical elements of BIG1 molecular structure were identified, as well as the potential function of microtubules in a novel PKA effect on BIG1 translocation...
  7. pmc Association of brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2) with recycling endosomes during transferrin uptake
    Xiaoyan Shen
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:2635-40. 2006
    ..We suggest that BIG2 has an important role in Tfn uptake and TfnR recycling, perhaps through its demonstrated interaction with Exo70 and the exocyst complex...
  8. pmc Nuclear localization and molecular partners of BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors
    Philip Ian Padilla
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 101:2752-7. 2004
    ..These data are consistent with independent intracellular movements and actions of BIG1 and BIG2, and they are also evidence of the participation of BIG1 in both Golgi and nuclear functions...
  9. pmc Unfolded protein response and cell death after depletion of brefeldin A-inhibited guanine nucleotide-exchange protein GBF1
    Carmen Citterio
    Translational Medicine Branch and Proteomics Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 105:2877-82. 2008
    ..Depletion of GBF1, but not BIG1 or BIG2, induced relocation of S2P from Golgi to ER with proteolysis of ATF6 followed by up-regulation of ER chaperones, mimicking a UPR response...
  10. pmc Lymphangioleiomyomatosis (LAM): molecular insights lead to targeted therapies
    Connie G Glasgow
    Translational Medicine Branch, NHLBI, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892 1590, USA
    Respir Med 104:S45-58. 2010
    ..Thus, insights into the molecular and biological properties of LAM cells and molecular phenotyping of patients with LAM have led to clinical trials of targeted therapies. Funded by the Intramural Research Program, NIH/NHLBI...
  11. pmc Erythropoietin-driven proliferation of cells with mutations in the tumor suppressor gene TSC2
    Yoshihiko Ikeda
    National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1590, USA
    Am J Physiol Lung Cell Mol Physiol 300:L64-72. 2011
    ..Although the high red cell mass of LAM patients could be related to advanced disease, we propose that EPO, synthesized in response to episodic hypoxia, may increase disease progression by enhancing the proliferation of LAM cells...
  12. pmc Phenotypic characterization of disseminated cells with TSC2 loss of heterozygosity in patients with lymphangioleiomyomatosis
    Xiong Cai
    Cardiovascular and Pulmonary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Am J Respir Crit Care Med 182:1410-8. 2010
    ....
  13. pmc The natural history of lymphangioleiomyomatosis: markers of severity, rate of progression and prognosis
    Angelo M Taveira-Dasilva
    Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1590, USA
    Lymphat Res Biol 8:9-19. 2010
    ....
  14. ncbi request reprint Giles F. Filley Lecture. Genetics and gene expression in lymphangioleiomyomatosis
    Gustavo Pacheco-Rodriguez
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1590, USA
    Chest 121:56S-60S. 2002
    ....
  15. pmc Chemokine-enhanced chemotaxis of lymphangioleiomyomatosis cells with mutations in the tumor suppressor TSC2 gene
    Gustavo Pacheco-Rodriguez
    Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 182:1270-7. 2009
    ..g., breast cancer and melanoma cells). These observations are consistent with the notion that chemokines such as CCL2 may serve to determine mobility and specify the site of metastasis of the LAM cell...
  16. pmc Association of guanine nucleotide-exchange protein BIG1 in HepG2 cell nuclei with nucleolin, U3 snoRNA, and fibrillarin
    Philip Ian Padilla
    Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 105:3357-61. 2008
    ..Nuclear functions of BIG1 remain to be determined...
  17. pmc Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity
    Ermanno Puxeddu
    Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:6158-63. 2009
    ..Thus, PDE3A in these BIG1 and BIG2 AKAP complexes may contribute to the regulation of ARF function via limitation of cAMP effects with spatial and temporal specificity...
  18. ncbi request reprint Cytohesin-1: structure, function, and ARF activation
    Gustavo Pacheco-Rodriguez
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Methods Enzymol 404:184-95. 2005
    ..This chapter is concerned for the most part with cytohesin-1 and the assay of its GEP activity...
  19. pmc Interaction of BIG2, a brefeldin A-inhibited guanine nucleotide-exchange protein, with exocyst protein Exo70
    Kai feng Xu
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:2784-9. 2005
    ....
  20. pmc The role of chemokines in migration of metastatic-like lymphangioleiomyomatosis cells
    Gustavo Pacheco-Rodriguez
    Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1590, USA
    Crit Rev Immunol 30:387-94. 2010
    ..Chemokines have been implicated in tumor metastasis, and our data suggest a role for chemokines in LAM cell mobilization and thereby in the pathogenesis of LAM...
  21. pmc Effects of prolactin on TSC2-null Eker rat cells and in pulmonary lymphangioleiomyomatosis
    Yasuhiro Terasaki
    Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1590, USA
    Am J Respir Crit Care Med 182:531-9. 2010
    ..Growth factors involved in lymphangioleiomyomatosis cell proliferation are unknown. Prolactin, an important reproductive hormone in women, is known to promote cell proliferation and survival in other tissues...
  22. pmc Molecular and genetic analysis of disseminated neoplastic cells in lymphangioleiomyomatosis
    Denise M Crooks
    Pulmonary Critical Care Medicine Branch and Flow Cytometry Core, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 101:17462-7. 2004
    ....
  23. pmc MCP-1 overexpressed in tuberous sclerosis lesions acts as a paracrine factor for tumor development
    Shaowei Li
    Department of Dermatology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    J Exp Med 202:617-24. 2005
    ..Together these findings suggest that MCP-1 is an important paracrine factor for TSC tumorigenesis and may be a new therapeutic target...
  24. pmc Lymphangioleiomyomatosis and TSC2-/- cells
    Thomas N Darling
    Department of Dermatology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    Lymphat Res Biol 8:59-69. 2010
    ..Further improvements in the ability to reliably grow well-characterized TSC2(-/-) cells from human tumors are critical to developing in vitro and in vivo model systems for studies of LAM pathogenesis and treatment...
  25. ncbi request reprint ARF-directed guanine-nucleotide-exchange (GEP) proteins
    Gustavo Pacheco-Rodriguez
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 189:181-9. 2002
  26. pmc Tissue-specific renin-angiotensin system in pulmonary lymphangioleiomyomatosis
    Julio C Valencia
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Am J Respir Cell Mol Biol 35:40-7. 2006
    ..A LAM-specific renin-angiotensin system appears to function within the LAM nodule as an autocrine system that could promote LAM cell proliferation and migration, and could represent a pharmacologic target...
  27. ncbi request reprint LY303511 (2-piperazinyl-8-phenyl-4H-1-benzopyran-4-one) acts via phosphatidylinositol 3-kinase-independent pathways to inhibit cell proliferation via mammalian target of rapamycin (mTOR)- and non-mTOR-dependent mechanisms
    Arnold S Kristof
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    J Pharmacol Exp Ther 314:1134-43. 2005
    ..Given its inhibition of cell proliferation via mTOR-dependent and independent mechanisms, LY303511 has therapeutic potential with antineoplastic actions that are independent of PI3K inhibition...
  28. pmc Mesenchymal-epithelial interactions involving epiregulin in tuberous sclerosis complex hamartomas
    Shaowei Li
    Department of Dermatology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814 4712, USA
    Proc Natl Acad Sci U S A 105:3539-44. 2008
    ..These results suggest that hamartomatous TSC skin tumors are induced by paracrine factors released by two-hit cells in the dermis and that proliferation with mTOR activation of the overlying epidermis is an effect of epiregulin...
  29. pmc Involvement of lymphatics in lymphangioleiomyomatosis
    Connie G Glasgow
    Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1590, USA
    Lymphat Res Biol 7:221-8. 2009
    ..e., lymphangioleiomyoma, adenopathy) than in healthy volunteers or LAM patients with cystic disease limited to the lung. These findings are consistent with an important function for lymphangiogenesis in LAM...
  30. pmc ARL4D recruits cytohesin-2/ARNO to modulate actin remodeling
    Chun Chun Li
    Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan
    Mol Biol Cell 18:4420-37. 2007
    ..Together, these data demonstrate that ARL4D acts as a novel upstream regulator of cytohesin-2/ARNO to promote ARF6 activation and modulate actin remodeling...