David E Ott

Summary

Affiliation: National Cancer Institute
Country: USA

Publications

  1. pmc Equine infectious anemia virus and the ubiquitin-proteasome system
    David E Ott
    AIDS Vaccine Program, SAIC Frederick, National Cancer Institute at Frederick, National Institutes of Health, Frederick, Maryland 21702, USA
    J Virol 76:3038-44. 2002
  2. doi request reprint Cellular proteins detected in HIV-1
    David E Ott
    AIDS Vaccine Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    Rev Med Virol 18:159-75. 2008
  3. pmc Highly conserved serine residue 40 in HIV-1 p6 regulates capsid processing and virus core assembly
    Jörg Votteler
    Institute of Virology, Friedrich Alexander University, Erlangen, Germany
    Retrovirology 8:11. 2011
  4. pmc Redundant roles for nucleocapsid and matrix RNA-binding sequences in human immunodeficiency virus type 1 assembly
    David E Ott
    Basic Research Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    J Virol 79:13839-47. 2005
  5. pmc Heterologous late-domain sequences have various abilities to promote budding of human immunodeficiency virus type 1
    David E Ott
    Basic Research Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland 21702 1201, USA
    J Virol 79:9038-45. 2005
  6. pmc Mutational analysis of the C-terminal gag cleavage sites in human immunodeficiency virus type 1
    Lori V Coren
    AIDS Vaccine Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    J Virol 81:10047-54. 2007
  7. pmc The nucleocapsid region of human immunodeficiency virus type 1 Gag assists in the coordination of assembly and Gag processing: role for RNA-Gag binding in the early stages of assembly
    David E Ott
    AIDS and Cancer Virus Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    J Virol 83:7718-27. 2009
  8. pmc Elimination of protease activity restores efficient virion production to a human immunodeficiency virus type 1 nucleocapsid deletion mutant
    David E Ott
    AIDS Vaccine Program, SAIC Frederick Inc, National Cancer Institute at Frederick, National Institutes of Health, Frederick, Maryland 21702 1201, USA
    J Virol 77:5547-56. 2003
  9. pmc Retroviruses have differing requirements for proteasome function in the budding process
    David E Ott
    AIDS Vaccine Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland 21702 1201, USA
    J Virol 77:3384-93. 2003
  10. ncbi request reprint Potential roles of cellular proteins in HIV-1
    David E Ott
    AIDS Vaccine Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland 21702 1201, USA
    Rev Med Virol 12:359-74. 2002

Collaborators

Detail Information

Publications33

  1. pmc Equine infectious anemia virus and the ubiquitin-proteasome system
    David E Ott
    AIDS Vaccine Program, SAIC Frederick, National Cancer Institute at Frederick, National Institutes of Health, Frederick, Maryland 21702, USA
    J Virol 76:3038-44. 2002
    ..This suggests that, although its Gag is monoubiquitinated, the requirements for EIAV release are somewhat different from those for retroviruses that are sensitive to proteasome inhibitors...
  2. doi request reprint Cellular proteins detected in HIV-1
    David E Ott
    AIDS Vaccine Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    Rev Med Virol 18:159-75. 2008
    ..In the same way, knowledge of viral--cellular protein interactions might assist our understanding of important cellular pathways...
  3. pmc Highly conserved serine residue 40 in HIV-1 p6 regulates capsid processing and virus core assembly
    Jörg Votteler
    Institute of Virology, Friedrich Alexander University, Erlangen, Germany
    Retrovirology 8:11. 2011
    ..In this study, we investigated the importance of the highly conserved serine residue at position 40, which until now has not been assigned to any known function of p6...
  4. pmc Redundant roles for nucleocapsid and matrix RNA-binding sequences in human immunodeficiency virus type 1 assembly
    David E Ott
    Basic Research Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    J Virol 79:13839-47. 2005
    ..Together, these results support an RNA-binding requirement for Gag assembly, which relies on binding of RNA by MA or NC sequences to condense, organize, and stabilize the HIV-1 Gag-Gag interactions that form the virion...
  5. pmc Heterologous late-domain sequences have various abilities to promote budding of human immunodeficiency virus type 1
    David E Ott
    Basic Research Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland 21702 1201, USA
    J Virol 79:9038-45. 2005
    ..These results show that L-domain sequences are not necessarily interchangeable. Thus, HIV-1 Gag might have a more stringent requirement for L-domain function than the other retroviruses previously studied...
  6. pmc Mutational analysis of the C-terminal gag cleavage sites in human immunodeficiency virus type 1
    Lori V Coren
    AIDS Vaccine Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    J Virol 81:10047-54. 2007
    ..In turn, this finding suggests an important role for the p9(NC/SP2) species in some aspect of integration...
  7. pmc The nucleocapsid region of human immunodeficiency virus type 1 Gag assists in the coordination of assembly and Gag processing: role for RNA-Gag binding in the early stages of assembly
    David E Ott
    AIDS and Cancer Virus Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
    J Virol 83:7718-27. 2009
    ..Therefore, NC plays an important initiating role in Gag assembly...
  8. pmc Elimination of protease activity restores efficient virion production to a human immunodeficiency virus type 1 nucleocapsid deletion mutant
    David E Ott
    AIDS Vaccine Program, SAIC Frederick Inc, National Cancer Institute at Frederick, National Institutes of Health, Frederick, Maryland 21702 1201, USA
    J Virol 77:5547-56. 2003
    ..These results show that HIV-1 can form particles efficiently without NC...
  9. pmc Retroviruses have differing requirements for proteasome function in the budding process
    David E Ott
    AIDS Vaccine Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland 21702 1201, USA
    J Virol 77:3384-93. 2003
    ....
  10. ncbi request reprint Potential roles of cellular proteins in HIV-1
    David E Ott
    AIDS Vaccine Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland 21702 1201, USA
    Rev Med Virol 12:359-74. 2002
    ..The extensive study of HIV-1 has already brought increased attention to the fields of immunology and vaccine science and, in the same way, might assist our understanding of the cellular pathways themselves...
  11. pmc Nef-mediated MHC class I down-regulation unmasks clonal differences in virus suppression by SIV-specific CD8(+) T cells independent of IFN-gamma and CD107a responses
    Jacob T Minang
    AIDS and Cancer Virus Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    Virology 391:130-9. 2009
    ..These data emphasize that current assays for evaluating CTL responses in infected or vaccinated individuals do not fully capture the complex requirements for effective CTL-mediated control of virus replication...
  12. pmc The Mamu B 17-restricted SIV Nef IW9 to TW9 mutation abrogates correct epitope processing and presentation without loss of replicative fitness
    Jacob T Minang
    AIDS and Cancer Virus Program, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
    Virology 375:307-14. 2008
    ..Our data highlight the value of functional assays using virus-infected target cells as opposed to peptide-pulsed APC when assessing relevant escape mutations in CTL epitopes...
  13. pmc Real-time visualization of HIV-1 GAG trafficking in infected macrophages
    Karine Gousset
    Virus Cell Interaction Section, HIV Drug Resistance Program, National Cancer Institute, Frederick, Maryland, USA
    PLoS Pathog 4:e1000015. 2008
    ..These data indicate that a population of Gag in infected macrophages remains sequestered internally and is presented to uninfected target cells at a virological synapse...
  14. pmc TCR triggering transcriptionally downregulates CCR5 expression on rhesus macaque CD4(+) T-cells with no measurable effect on susceptibility to SIV infection
    Jacob T Minang
    AIDS and Cancer Virus Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    Virology 409:132-40. 2011
    ..Our data suggest a model where i) very low levels of CCR5 are sufficient for efficient SIV infection, ii) CCR5 levels above this threshold do not enhance infection, and iii) low level infection can occur in the absence of CCR5...
  15. pmc Proteomic and biochemical analysis of purified human immunodeficiency virus type 1 produced from infected monocyte-derived macrophages
    Elena Chertova
    AIDS Vaccine Research Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    J Virol 80:9039-52. 2006
    ..MDM-derived HIV contained 26 of 37 proteins previously found in exosomes, consistent with the idea that HIV uses the late endosome/multivesicular body pathway during virion budding from macrophages...
  16. pmc CD45 immunoaffinity depletion of vesicles from Jurkat T cells demonstrates that exosomes contain CD45: no evidence for a distinct exosome/HIV-1 budding pathway
    Lori V Coren
    AIDS and Cancer Virus Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland 21702 1201, USA
    Retrovirology 5:64. 2008
    ..The presence of CD45 on vesicles, including exosomes, and its absence on virions argues against a specialized budding pathway that is shared by both exosomes and HIV-1...
  17. pmc Transduction of SIV-specific TCR genes into rhesus macaque CD8+ T cells conveys the ability to suppress SIV replication
    Eugene V Barsov
    AIDS and Cancer Virus Program, SAIC Frederick Inc, National Cancer Institute at Frederick, Frederick, Maryland, United States of America
    PLoS ONE 6:e23703. 2011
    ....
  18. ncbi request reprint Stoichiometry of the antiviral protein APOBEC3G in HIV-1 virions
    Hongzhan Xu
    Viral Mutation Section, HIV Drug Resistance Program, Center for Cancer Research, National Cancer Institute Frederick, P O Box B, Bldg 535, Rm 334, Frederick, MD 21702, USA
    Virology 360:247-56. 2007
    ..These results indicate that virion incorporation of only a few molecules of A3G is sufficient to inhibit HIV-1 replication...
  19. ncbi request reprint Efficient inhibition of SIV replication in rhesus CD4+ T-cell clones by autologous immortalized SIV-specific CD8+ T-cell clones
    Jacob T Minang
    AIDS Vaccine Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702, USA
    Virology 372:430-41. 2008
    ..The use of hTERT immortalized effector and target cells for such assays preserves relevant functional properties while providing a convenient, reproducible means of conducting studies over time...
  20. pmc Elimination of retroviral infectivity by N-ethylmaleimide with preservation of functional envelope glycoproteins
    David R Morcock
    AIDS Vaccine Program, SAIC Frederick, NCI Frederick, Building 535, 5th Floor, PO Box B, Frederick, MD 21702, USA
    J Virol 79:1533-42. 2005
    ....
  21. ncbi request reprint Transduction with human telomerase reverse transcriptase immortalizes a rhesus macaque CD8+ T cell clone with maintenance of surface marker phenotype and function
    Hanne Andersen
    AIDS Vaccine Program, SAIC Frederick NCI Frederick, Frederick, Maryland 21702, USA
    AIDS Res Hum Retroviruses 23:456-65. 2007
    ..Such immortalized T cells may be useful as a source of consistent controls for in vitro assays of cellular immune function, and as a potentially important reagent for autologous adoptive cellular immunotherapy studies in macaques...
  22. pmc Distribution, persistence, and efficacy of adoptively transferred central and effector memory-derived autologous simian immunodeficiency virus-specific CD8+ T cell clones in rhesus macaques during acute infection
    Jacob T Minang
    AIDS and Cancer Virus Program, SAIC Frederick, Frederick, MD 21702, USA
    J Immunol 184:315-26. 2010
    ....
  23. ncbi request reprint Human immunodeficiency virus type 1 preferentially encapsidates genomic RNAs that encode Pr55(Gag): functional linkage between translation and RNA packaging
    Dexter T K Poon
    AIDS Vaccine Program, National Cancer Institute Frederick, National Institutes of Health, Frederick, Maryland 21702 1201, USA
    Virology 293:368-78. 2002
    ..Together, our data link translation and particle formation to the packaging of viral RNA and support a model of cis packaging where nascent Gag proteins encapsidate their cognate RNA...
  24. pmc Quantitation of HLA class II protein incorporated into human immunodeficiency type 1 virions purified by anti-CD45 immunoaffinity depletion of microvesicles
    Charles M Trubey
    Basic Research Program Research Technology Program, SAIC Frederick, Inc, National Cancer Institute Frederick, Frederick, Maryland 21702 1201, USA
    J Virol 77:12699-709. 2003
    ..Our observations demonstrate the utility of CD45 immunoaffinity-based approaches for producing highly purified retrovirus preparations for applications that would benefit from the use of virus that is essentially free of microvesicles...
  25. pmc Efficiency of human immunodeficiency virus type 1 postentry infection processes: evidence against disproportionate numbers of defective virions
    James A Thomas
    AIDS Vaccine Program, SAIC Frederick, Inc, Building 535, 4th Floor, National Cancer Institute, Frederick, MD 21702, USA
    J Virol 81:4367-70. 2007
    ..The apparent predominance of noninfectious particles is due to infrequent occurrences of successful virus-cell interactions...
  26. pmc Human immunodeficiency virus type 1-induced macrophage gene expression includes the p21 gene, a target for viral regulation
    Nancy Vazquez
    National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    J Virol 79:4479-91. 2005
    ..These data implicate p21 as a pivotal macrophage facilitator of the viral life cycle. Moreover, regulators of p21, such as CDDO, may provide an interventional approach to modulate HIV-1 replication...
  27. ncbi request reprint Fusion proteins with COOH-terminal ubiquitin are stable and maintain dual functionality in vivo
    Shu Bing Qian
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 0440, USA
    J Biol Chem 277:38818-26. 2002
    ..The multifunctionality of X-Ub fusion proteins opens the possibility for a number of novel practical applications, including the imaging of Ub conjugate formation in living cells...
  28. doi request reprint Purification of HIV-1 virions by subtilisin digestion or CD45 immunoaffinity depletion for biochemical studies
    David E Ott
    AIDS Vaccine Program, SAIC Frederick, Inc, NCI Frederick, Frederick, MD, USA
    Methods Mol Biol 485:15-25. 2009
    ....
  29. pmc Small-molecule inactivation of HIV-1 NCp7 by repetitive intracellular acyl transfer
    Lisa M Miller Jenkins
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    Nat Chem Biol 6:887-9. 2010
    ..The thiol produced by SAMT reaction with NCp7 is acetylated by cellular enzymes to regenerate active SAMTs via a recycling mechanism unique among small-molecule inhibitors of HIV...
  30. ncbi request reprint Capture of antigen-specific T lymphocytes from human blood by selective immortalization to establish long-term T-cell lines maintaining primary cell characteristics
    Eugene V Barsov
    AIDS Vaccine Program, SAIC Frederick, Inc, NCI Frederick, P O Box B, Frederick, MD 21702, USA
    Immunol Lett 105:26-37. 2006
    ..This approach provides a convenient, reproducible means for generating a stable, continuously renewable source of antigen-specific T lymphocytes for a variety of studies of T cell biology...
  31. pmc Physiological levels of virion-associated human immunodeficiency virus type 1 envelope induce coreceptor-dependent calcium flux
    Marta Melar
    Northwestern University, Department of Cell and Molecular Biology, Feinberg School of Medicine, Ward 8 140, 303 E Chicago Ave, Chicago, IL 60611, USA
    J Virol 81:1773-85. 2007
    ..These results indicate that the physiological levels of virion binding can activate signaling in CD4(+) T cells in vivo and therefore might contribute to HIV-induced pathogenesis...
  32. pmc Retroviruses human immunodeficiency virus and murine leukemia virus are enriched in phosphoinositides
    Robin Chan
    Centre for Life Sciences CeLS, Yong Loo Lin School of Medicine, National University of Singapore, 28 Medical Drive, Level 04 21, Singapore 117607, Singapore
    J Virol 82:11228-38. 2008
    ..Overall, these results suggest that HIV-1 and other retroviruses bud from cholesterol-rich regions of the plasma membrane and exploit matrix/PI(4,5)P(2) interactions for particle release from cells...
  33. pmc Dynamic fluorescent imaging of human immunodeficiency virus type 1 gag in live cells by biarsenical labeling
    Lynnie Rudner
    Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, Vermont, USA
    J Virol 79:4055-65. 2005
    ..Taken together, our results show that Gag concentrates in specific plasma membrane areas rapidly after translation and demonstrate the utility of biarsenical labeling for visualizing the dynamic localization of Gag...