Barry R O'Keefe

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Antiviral lectins from red and blue-green algae show potent in vitro and in vivo activity against hepatitis C virus
    Yutaka Takebe
    AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan
    PLoS ONE 8:e64449. 2013
  2. pmc Broad-spectrum in vitro activity and in vivo efficacy of the antiviral protein griffithsin against emerging viruses of the family Coronaviridae
    Barry R O'Keefe
    Molecular Targets Development Program, Center for Cancer Research, NCI Frederick, Frederick, Maryland 217021, USA
    J Virol 84:2511-21. 2010
  3. ncbi request reprint High throughput screening for cyanovirin-N mimetics binding to HIV-1 gp41
    John A Beutler
    Intramural Research Support Program and Molecular Targets Drug Discovery Program, SAIC Frederick, Center for Cancer Research, National Cancer Institute Frederick, Frederick, MD 21702 1201, USA
    J Biomol Screen 7:105-10. 2002
  4. ncbi request reprint A potent novel anti-HIV protein from the cultured cyanobacterium Scytonema varium
    Heidi R Bokesch
    Molecular Targets Discovery Program, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, Maryland 21702 1201, USA
    Biochemistry 42:2578-84. 2003
  5. ncbi request reprint Domain-swapped structure of the potent antiviral protein griffithsin and its mode of carbohydrate binding
    Natasza E Ziółkowska
    Protein Structure Section, Macromolecular Crystallography Laboratory, National Cancer Institute, NCI Frederick, Frederick, MD 21702, USA
    Structure 14:1127-35. 2006
  6. ncbi request reprint Multisite and multivalent binding between cyanovirin-N and branched oligomannosides: calorimetric and NMR characterization
    Shilpa R Shenoy
    Molecular Targets Discovery Program, NCI Center for Cancer Research, National Cancer Institute, NCI Frederick, Frederick, MD 21702, USA
    Chem Biol 9:1109-18. 2002
  7. ncbi request reprint Potent anti-HIV activity of scytovirin domain 1 peptide
    Changyun Xiong
    Molecular Targets Development Program, National Cancer Institute Frederick, National Institutes of Health, Bldg 562 Rm 201, Fort Detrick, Frederick, MD 21702, USA
    Peptides 27:1668-75. 2006
  8. ncbi request reprint Structures of the complexes of a potent anti-HIV protein cyanovirin-N and high mannose oligosaccharides
    Istvan Botos
    Macromolecular Crystallography Laboratory, National Cancer Institute National Institutes of Health, MCL Building 536, Frederick, MD 21702 1201, USA
    J Biol Chem 277:34336-42. 2002
  9. pmc Potent anti-influenza activity of cyanovirin-N and interactions with viral hemagglutinin
    Barry R O'Keefe
    Molecular Targets Development Program, Center for Cancer Research, Frederick, Maryland 21702, USA
    Antimicrob Agents Chemother 47:2518-25. 2003
  10. ncbi request reprint Isolation and characterization of anti-HIV peptides from Dorstenia contrajerva and Treculia obovoidea
    Heidi R Bokesch
    Basic Research Program, SAIC Frederick, and Molecular Targets Development Program, Center for Cancer Research, National Cancer Institute Frederick, National Institutes of Health, MD 21702 1201, USA
    FEBS Lett 567:287-90. 2004

Collaborators

Detail Information

Publications22

  1. pmc Antiviral lectins from red and blue-green algae show potent in vitro and in vivo activity against hepatitis C virus
    Yutaka Takebe
    AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan
    PLoS ONE 8:e64449. 2013
    ..These results indicate that HCV viral entry inhibitors can be an effective component of anti-HCV therapy and that these proteins should be studied further for their therapeutic potential...
  2. pmc Broad-spectrum in vitro activity and in vivo efficacy of the antiviral protein griffithsin against emerging viruses of the family Coronaviridae
    Barry R O'Keefe
    Molecular Targets Development Program, Center for Cancer Research, NCI Frederick, Frederick, Maryland 217021, USA
    J Virol 84:2511-21. 2010
    ....
  3. ncbi request reprint High throughput screening for cyanovirin-N mimetics binding to HIV-1 gp41
    John A Beutler
    Intramural Research Support Program and Molecular Targets Drug Discovery Program, SAIC Frederick, Center for Cancer Research, National Cancer Institute Frederick, Frederick, MD 21702 1201, USA
    J Biomol Screen 7:105-10. 2002
    ..A limited set of extracts was selected for bioassay-guided fractionation...
  4. ncbi request reprint A potent novel anti-HIV protein from the cultured cyanobacterium Scytonema varium
    Heidi R Bokesch
    Molecular Targets Discovery Program, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, Maryland 21702 1201, USA
    Biochemistry 42:2578-84. 2003
    ..This unique protein consists of a single 95-amino acid chain with significant internal sequence duplication and 10 cysteines forming five intrachain disulfide bonds...
  5. ncbi request reprint Domain-swapped structure of the potent antiviral protein griffithsin and its mode of carbohydrate binding
    Natasza E Ziółkowska
    Protein Structure Section, Macromolecular Crystallography Laboratory, National Cancer Institute, NCI Frederick, Frederick, MD 21702, USA
    Structure 14:1127-35. 2006
    ..Antiviral potency of griffithsin is likely due to the presence of multiple, similar sugar binding sites that provide redundant attachment points for complex carbohydrate molecules present on viral envelopes...
  6. ncbi request reprint Multisite and multivalent binding between cyanovirin-N and branched oligomannosides: calorimetric and NMR characterization
    Shilpa R Shenoy
    Molecular Targets Discovery Program, NCI Center for Cancer Research, National Cancer Institute, NCI Frederick, Frederick, MD 21702, USA
    Chem Biol 9:1109-18. 2002
    ..This is the first detailed analysis of a biologically relevant interaction between cyanovirin-N and high-mannose oligosaccharides of HIV-1 gp120...
  7. ncbi request reprint Potent anti-HIV activity of scytovirin domain 1 peptide
    Changyun Xiong
    Molecular Targets Development Program, National Cancer Institute Frederick, National Institutes of Health, Bldg 562 Rm 201, Fort Detrick, Frederick, MD 21702, USA
    Peptides 27:1668-75. 2006
    ....
  8. ncbi request reprint Structures of the complexes of a potent anti-HIV protein cyanovirin-N and high mannose oligosaccharides
    Istvan Botos
    Macromolecular Crystallography Laboratory, National Cancer Institute National Institutes of Health, MCL Building 536, Frederick, MD 21702 1201, USA
    J Biol Chem 277:34336-42. 2002
    ..These structures show unequivocally the binding geometry of high mannose sugars to CV-N, permitting a better understanding of carbohydrate binding to this potential new lead for the design of drugs against AIDS...
  9. pmc Potent anti-influenza activity of cyanovirin-N and interactions with viral hemagglutinin
    Barry R O'Keefe
    Molecular Targets Development Program, Center for Cancer Research, Frederick, Maryland 21702, USA
    Antimicrob Agents Chemother 47:2518-25. 2003
    ..These results further reveal new potential avenues for antiviral therapeutics and prophylaxis targeting specific oligosaccharide-comprised sites on certain enveloped viruses, including HIV, influenza virus, and possibly others...
  10. ncbi request reprint Isolation and characterization of anti-HIV peptides from Dorstenia contrajerva and Treculia obovoidea
    Heidi R Bokesch
    Basic Research Program, SAIC Frederick, and Molecular Targets Development Program, Center for Cancer Research, National Cancer Institute Frederick, National Institutes of Health, MD 21702 1201, USA
    FEBS Lett 567:287-90. 2004
    ..Both peptides were shown to bind to gp41 and gp120 and to inhibit the cytopathic effects of HIV-1(RF) infection in a human T-lymphoblastoid cell line (CEM-SS)...
  11. pmc Monomerization of viral entry inhibitor griffithsin elucidates the relationship between multivalent binding to carbohydrates and anti-HIV activity
    Tinoush Moulaei
    Protein Structure Section, Macromolecular Crystallography Laboratory, National Cancer Institute Frederick, Frederick, MD 21702 1201, USA
    Structure 18:1104-15. 2010
    ..Atomic resolution crystal structure of a complex between mGRFT and nonamannoside revealed that a single mGRFT molecule binds to two different nonamannoside molecules through all three carbohydrate-binding sites present on the monomer...
  12. ncbi request reprint Overexpression and purification of scytovirin, a potent, novel anti-HIV protein from the cultured cyanobacterium Scytonema varium
    Changyun Xiong
    Molecular Targets Development Program, National Cancer Institute Frederick, National Institutes of Health, Frederick, MD, USA
    Protein Expr Purif 46:233-9. 2006
    ..The current system resulted in yields of 5-10mg/L of purified SVN for structural studies and for preclinical development of SVN as a topical microbicide for HIV prophylaxis...
  13. ncbi request reprint Crystallographic, thermodynamic, and molecular modeling studies of the mode of binding of oligosaccharides to the potent antiviral protein griffithsin
    Natasza E Ziółkowska
    Protein Structure Section, Macromolecular Crystallography Laboratory, National Cancer Institute, NCI Frederick, Frederick, Maryland 21702 1201, USA
    Proteins 67:661-70. 2007
    ..The ability to mediate tight multivalent and multisite interactions with high-mannose oligosaccharides helps to explain the potent antiviral activity of griffithsin...
  14. pmc Differential inhibitory effects of cyanovirin-N, griffithsin, and scytovirin on entry mediated by envelopes of gammaretroviruses and deltaretroviruses
    Stig M R Jensen
    Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, Frederick, Maryland, USA
    J Virol 88:2327-32. 2014
    ..For gammaretroviruses, CV-N inhibited entry mediated by some but not all of the envelopes examined, whereas GRFT and SVN displayed only little or no effect. ..
  15. ncbi request reprint Development of a fluorescent microplate assay for determining cyanovirin-N levels in plasma
    Scott D Bringans
    Molecular Targets Development Program, Center for Cancer Research, National Cancer Institute Frederick, Building 562, Room 201, Frederick 21702 1201, MD, USA
    Anal Bioanal Chem 380:269-74. 2004
    ..The results of these studies confirmed the reliability and sensitivity of this assay and the feasibility of its use for pharmacokinetic studies in a variety of species...
  16. pmc Scaleable manufacture of HIV-1 entry inhibitor griffithsin and validation of its safety and efficacy as a topical microbicide component
    Barry R O'Keefe
    Molecular Targets Development Program, National Cancer Institute at Frederick, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 106:6099-104. 2009
    ..Moreover, GRFT-P is potently active in preventing infection of cervical explants by HIV-1 and has no mitogenic activity on cultured human lymphocytes...
  17. pmc Structure-activity analysis of vinylogous urea inhibitors of human immunodeficiency virus-encoded ribonuclease H
    Suhman Chung
    RT Biochemistry Section, HIV Drug Resistance Program, National Cancer Institute Frederick, Frederick, Maryland 21702, USA
    Antimicrob Agents Chemother 54:3913-21. 2010
    ..Finally, single-molecule spectroscopy indicates that vinylogous ureas have the property of altering the reverse transcriptase orientation on a model RNA-DNA hybrid mimicking initiation plus-strand DNA synthesis...
  18. ncbi request reprint Cyclonellin, a new cyclic octapeptide from the marine sponge Axinella carteri
    Dennis J Milanowski
    Molecular Targets Development Program, Center for Cancer Research, National Cancer Institute, Building 1052, Room 121, Frederick, Maryland 21702 1201, USA
    J Nat Prod 67:441-4. 2004
    ..The absolute configurations of the constituent amino acids were determined by acid hydrolysis, derivitization with FDAA, and LC-MS analyses...
  19. ncbi request reprint Isolation and characterization of griffithsin, a novel HIV-inactivating protein, from the red alga Griffithsia sp
    Toshiyuki Mori
    Molecular Targets Development Program, Center for Cancer Research, NCI Frederick, Maryland 21702, USA
    J Biol Chem 280:9345-53. 2005
    ..GRFT could be a potential candidate microbicide to prevent the sexual transmission of HIV and AIDS...
  20. pmc Topology of the disulfide bonds in the antiviral lectin scytovirin
    Tinoush Moulaei
    Protein Structure Section, Macromolecular Crystallography Laboratory, National Cancer Institute, NCI Frederick, Frederick, Maryland 21702 1201, USA
    Protein Sci 19:1649-61. 2010
    ..Biochemistry 2003;42:2578-2584). Our observations emphasize the fact that proteins such as SVN, with disulfide bonds in close proximity, require considerable precautions when being fragmented for the purpose of disulfide assignment...
  21. pmc Discovery of new pyridoacridine alkaloids from Lissoclinum cf. badium that inhibit the ubiquitin ligase activity of Hdm2 and stabilize p53
    Jason A Clement
    Molecular Targets Development Program, Center for Cancer Research, National Cancer Institute Frederick, National Institutes of Health, Frederick, MD 21702 1201, USA
    Bioorg Med Chem 16:10022-8. 2008
    ..badium. Lissoclinidine B inhibited ubiquitylation and degradation of p53, and selectively killed transformed cells harboring wild-type p53, suggesting this compound could be used to develop new treatments...
  22. pmc Vinylogous ureas as a novel class of inhibitors of reverse transcriptase-associated ribonuclease H activity
    Michaela Wendeler
    HIV Drug Resistance Program, National Cancer Institute, Frederick, Maryland, USA
    ACS Chem Biol 3:635-44. 2008
    ..NSC727447 thus represents a novel class of RNase H antagonists with a mechanism of action differing from active site, divalent metal-chelating inhibitors that have been reported...