Sergei Nechaev

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Global analysis of short RNAs reveals widespread promoter-proximal stalling and arrest of Pol II in Drosophila
    Sergei Nechaev
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Science 327:335-8. 2010
  2. ncbi request reprint Promoter-proximal Pol II: when stalling speeds things up
    Sergei Nechaev
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Cell Cycle 7:1539-44. 2008
  3. pmc Dissection of the bacteriophage T4 late promoter complex
    Sergei Nechaev
    Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    J Mol Biol 379:402-13. 2008
  4. pmc The elusive object of desire--interactions of bacteriophages and their hosts
    Sergei Nechaev
    Laboratory of Molecular Carcinogenesis, NIEHS NIH, Room D434, 111 Alexander Dr, Research Triangle Park, NC 27709, USA
    Curr Opin Microbiol 11:186-93. 2008
  5. pmc Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling
    Karen Adelman
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Proc Natl Acad Sci U S A 106:18207-12. 2009
  6. pmc The role of an upstream promoter interaction in initiation of bacterial transcription
    Sergei Nechaev
    Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093 0634, USA
    EMBO J 25:1700-9. 2006
  7. pmc NELF-mediated stalling of Pol II can enhance gene expression by blocking promoter-proximal nucleosome assembly
    Daniel A Gilchrist
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Genes Dev 22:1921-33. 2008
  8. pmc The bacteriophage T4 late-transcription coactivator gp33 binds the flap domain of Escherichia coli RNA polymerase
    Sergei Nechaev
    Division of Biological Sciences and Center for Molecular Genetics, University of California at San Diego, La Jolla, CA 92093 0634, USA
    Proc Natl Acad Sci U S A 101:17365-70. 2004
  9. pmc Pol II waiting in the starting gates: Regulating the transition from transcription initiation into productive elongation
    Sergei Nechaev
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Biochim Biophys Acta 1809:34-45. 2011
  10. ncbi request reprint Bacteriophage-induced modifications of host RNA polymerase
    Sergei Nechaev
    Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Dr, La Jolla, California 92093 0634, USA
    Annu Rev Microbiol 57:301-22. 2003

Collaborators

Detail Information

Publications19

  1. pmc Global analysis of short RNAs reveals widespread promoter-proximal stalling and arrest of Pol II in Drosophila
    Sergei Nechaev
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Science 327:335-8. 2010
    ..These results indicate that the intrinsic efficiency of early elongation can greatly affect gene expression...
  2. ncbi request reprint Promoter-proximal Pol II: when stalling speeds things up
    Sergei Nechaev
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Cell Cycle 7:1539-44. 2008
    ..Here we briefly summarize the current data on promoter-proximal Pol II stalling and discuss implications of this newly appreciated regulatory strategy...
  3. pmc Dissection of the bacteriophage T4 late promoter complex
    Sergei Nechaev
    Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    J Mol Biol 379:402-13. 2008
    ..gp55 and gp33 perform sigma-like functions, gp55 in promoter recognition and gp33 (as well as gp55) in enhancer recognition. gp33 operates the switch between these two pathways by repressing basal transcription...
  4. pmc The elusive object of desire--interactions of bacteriophages and their hosts
    Sergei Nechaev
    Laboratory of Molecular Carcinogenesis, NIEHS NIH, Room D434, 111 Alexander Dr, Research Triangle Park, NC 27709, USA
    Curr Opin Microbiol 11:186-93. 2008
    ..As a result, phages play central roles in a great variety of genetic processes and increase the rate of evolutionary change of the bacterial host, which could ultimately work to the benefit of the host in a long run...
  5. pmc Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling
    Karen Adelman
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Proc Natl Acad Sci U S A 106:18207-12. 2009
    ..We propose that polymerase stalling ensures the coordinated, timely activation the inflammatory gene expression program from Drosophila to mammals...
  6. pmc The role of an upstream promoter interaction in initiation of bacterial transcription
    Sergei Nechaev
    Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093 0634, USA
    EMBO J 25:1700-9. 2006
    ..We show that this upstream interaction is essential only at an early step of transcription initiation, and discuss the role of this interaction in promoter recognition and transcriptional regulation...
  7. pmc NELF-mediated stalling of Pol II can enhance gene expression by blocking promoter-proximal nucleosome assembly
    Daniel A Gilchrist
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Genes Dev 22:1921-33. 2008
    ..These findings identify a novel, positive role for stalled Pol II in regulating gene expression and suggest that there is a dynamic interplay between stalled Pol II and chromatin structure...
  8. pmc The bacteriophage T4 late-transcription coactivator gp33 binds the flap domain of Escherichia coli RNA polymerase
    Sergei Nechaev
    Division of Biological Sciences and Center for Molecular Genetics, University of California at San Diego, La Jolla, CA 92093 0634, USA
    Proc Natl Acad Sci U S A 101:17365-70. 2004
    ..We propose a model for the role of the gp45 sliding clamp in activation of T4 late-gene transcription...
  9. pmc Pol II waiting in the starting gates: Regulating the transition from transcription initiation into productive elongation
    Sergei Nechaev
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Biochim Biophys Acta 1809:34-45. 2011
    ..We also discuss the interplay between factors that affect early transcript elongation and the potential physiological consequences for genes that are regulated through transcriptional pausing...
  10. ncbi request reprint Bacteriophage-induced modifications of host RNA polymerase
    Sergei Nechaev
    Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Dr, La Jolla, California 92093 0634, USA
    Annu Rev Microbiol 57:301-22. 2003
    ..We interpret the biochemical and genetic data within the framework of a structure-function model of bacterial RNA polymerase and viral biology...
  11. pmc RNA polymerase is poised for activation across the genome
    Ginger W Muse
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Nat Genet 39:1507-11. 2007
    ..This finding indicates a role for regulation of polymerase elongation in the transcriptional responses to dynamic environmental and developmental cues...
  12. ncbi request reprint Purification and characterization of bacteriophage-encoded inhibitors of host RNA polymerase: T-odd phage gp2-like proteins
    Sergei Nechaev
    University of California San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    Methods Enzymol 370:212-25. 2003
  13. ncbi request reprint Beta subunit residues 186-433 and 436-445 are commonly used by Esigma54 and Esigma70 RNA polymerase for open promoter complex formation
    Siva R Wigneshweraraj
    Department of Biological Sciences, Imperial College of Science, Technology and Medicine, Biomedical Sciences Building, Imperial College Road, London SW7 2AZ, UK
    J Mol Biol 319:1067-83. 2002
    ..Late stages of promoter complex formation and localized melting of promoter DNA by Esigma70 and Esigma54 thus proceed through a common pathway...
  14. ncbi request reprint Mutations of bacterial RNA polymerase leading to resistance to microcin j25
    Julia Yuzenkova
    Department of Genetics, Waksman Institute, Piscataway, New Jersey 08854, USA
    J Biol Chem 277:50867-75. 2002
    ..We hypothesize that MccJ25 inhibits transcription by binding in RNAP secondary channel and blocking substrate access to the catalytic center...
  15. pmc Regulated communication between the upstream face of RNA polymerase and the beta' subunit jaw domain
    Siva R Wigneshweraraj
    Department of Biological Sciences, Imperial College London, London SW7 2AZ, UK
    EMBO J 23:4264-74. 2004
    ..Our results establish that accessory factors bound to the upstream face of the RNAP, communicate with the beta' jaw domain, and that such communication is subjected to regulation...
  16. ncbi request reprint A novel bacteriophage-encoded RNA polymerase binding protein inhibits transcription initiation and abolishes transcription termination by host RNA polymerase
    Sergei Nechaev
    Waksman Institute for Microbiology, Rutgers, The State University, Piscataway, NJ 08854, USA
    J Mol Biol 320:11-22. 2002
    ..Further physiological and mechanistic studies of this novel transcription factor should provide additional insights into its biological role and the processes of promoter recognition and transcription termination...
  17. ncbi request reprint Genome of Xanthomonas oryzae bacteriophage Xp10: an odd T-odd phage
    Julia Yuzenkova
    Waksman Institute for Microbiology, The State University, 190 Frelinghuysen Road, Piscataway, NJ 08854, USA
    J Mol Biol 330:735-48. 2003
    ....
  18. ncbi request reprint Enhancer-dependent transcription by bacterial RNA polymerase: the beta subunit downstream lobe is used by sigma 54 during open promoter complex formation
    Siva R Wigneshweraraj
    Department of Biological Sciences, National College of Science, Technology, and Medicine, SAFB, Imperial College Road, London SW7 2AZ, United Kingdom
    Methods Enzymol 370:646-57. 2003
  19. pmc RNA polymerase stalling at developmental control genes in the Drosophila melanogaster embryo
    Julia Zeitlinger
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Genet 39:1512-6. 2007
    ..We propose that Pol II stalling facilitates rapid temporal and spatial changes in gene activity during development...