Research Topics
Genomes and Genes | Geoffrey A MuellerSummaryAffiliation: National Institutes of Health Country: USA Publications
| Collaborators
|
Detail Information
Publications
Revised structure of the AbrB N-terminal domain unifies a diverse superfamily of putative DNA-binding proteinsBenjamin G Bobay
Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, 27695, USA
FEBS Lett 579:5669-74. 2005..This strongly suggests that MraZ also binds DNA and allows for a consensus model of DNA recognition by the members of this novel protein superfamily...
Structural studies of the PARP-1 BRCT domainPaul A Loeffler
Department of Chemistry, Sam Houston State University, Huntsville, Texas 77340, USA
BMC Struct Biol 11:37. 2011..The co-localization of XRCC1, as well as bound DNA repair factors, to sites of DNA damage is important for cell survival and genomic integrity...- A comparison of BRCT domains involved in nonhomologous end-joining: introducing the solution structure of the BRCT domain of polymerase lambdaGeoffrey A Mueller
Laboratory of Structural Biology, National Institute of Environmental Health Sciences, MD MR 01, Research Triangle Park, NC 27709, USA
DNA Repair (Amst) 7:1340-51. 2008..In conclusion, both the structure of the Pol lambda BRCT domain and its mode of interaction with the other components of the NHEJ complex significantly differ from the two previously studied homologs, Pol mu and TdT... 
Analytical solution to the coupled evolution of multidimensional NMR dataGeoffrey A Mueller
National Institute of Environment Health Sciences, 111 T W Alexander Drive, MD MR 01, Research Triangle Park, NC 27709, USA
J Biomol NMR 44:13-23. 2009..This algorithm is shown to be robust in that erroneous solutions give rise to large artifacts...- Solution structure of the Drosha double-stranded RNA-binding domainGeoffrey A Mueller
Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA
Silence 1:2. 2010..To gain insight into the function of this domain, we determined the nuclear magnetic resonance (NMR) solution structure... - The structure of the dust mite allergen Der p 7 reveals similarities to innate immune proteinsGeoffrey A Mueller
Laboratory of Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA
J Allergy Clin Immunol 125:909-917.e4. 2010..Allergen function might contribute to allergenicity, as shown for the protease activity of group 1 mite allergens and the interaction with the innate immune system by group 2 mite allergens... - Der p 5 crystal structure provides insight into the group 5 dust mite allergensGeoffrey A Mueller
Laboratory of Structural Biology, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
J Biol Chem 285:25394-401. 2010..Many allergens are known to bind hydrophobic ligands, which are thought to stimulate the innate immune system and have adjuvant-like effects on IgE-mediated inflammatory responses... - Ara h 2: crystal structure and IgE binding distinguish two subpopulations of peanut allergic patients by epitope diversityG A Mueller
Laboratory of Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA
Allergy 66:878-85. 2011..Peanut allergy affects 1% of the population and causes the most fatal food-related anaphylactic reactions. The protein Ara h 2 is the most potent peanut allergen recognized by 80-90% of peanut allergic patients... - NMR analysis of [methyl-13C]methionine UvrB from Bacillus caldotenax reveals UvrB-domain 4 heterodimer formation in solutionMatthew J DellaVecchia
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, USA
J Mol Biol 373:282-95. 2007..In summary, UvrB homodimerization is disfavored, while domain 4 homodimerization and UvrB-domain 4 heterodimerization are allowed... - Nuclear magnetic resonance solution structure of the Escherichia coli DNA polymerase III theta subunitGeoffrey A Mueller
Laboratory of Structural Biology, MR 01, National Institute of Environmental Health Sciences, 111 Alexander Drive, Research Triangle Park, North Carolina 27709, USA
J Bacteriol 187:7081-9. 2005..The new theta structure is expected to provide additional insight into its physiological role and its effect on the epsilon proofreading subunit... - Solution structure of polymerase mu's BRCT Domain reveals an element essential for its role in nonhomologous end joiningEugene F Derose
Laboratory of Structural Biology, National Institute of Environmental Health Sciences, 111 T W Alexander Drive, Research Triangle Park, North Carolina 27709, USA
Biochemistry 46:12100-10. 2007..Further, the similarity between the interaction regions of the BRCT domains of pol mu and TdT support the conclusion that they participate in NHEJ as alternate polymerases... - Metal-induced DNA translocation leads to DNA polymerase conformational activationThomas W Kirby
Laboratory of Structural Biology, NIEHS, Research Triangle Park, NC 27709, USA
Nucleic Acids Res 40:2974-83. 2012..These findings provide new insight into the relationships between conformational activation, enzyme activity and polymerase fidelity... - Solution structure of the lyase domain of human DNA polymerase lambdaEugene F Derose
Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Box 12233, Research Triangle Park, North Carolina 27709, USA
Biochemistry 42:9564-74. 2003..g., K60 in pol beta and K307 in pol lambda. Additionally, on the basis of the structural alignment obtained, several previously proposed mechanistic hypotheses can be evaluated... - Dependence of amino acid side chain 13C shifts on dihedral angle: application to conformational analysisRobert E London
Laboratory of Structural Biology, National Institute of Environmental Health Sciences, 111 T W Alexander Drive, MD MR 01, Research Triangle Park, North Carolina 27709, USA
J Am Chem Soc 130:11097-105. 2008.... - Homodimerization of the p51 subunit of HIV-1 reverse transcriptaseXunhai Zheng
Laboratory of Structural Biology, MR 01, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
Biochemistry 49:2821-33. 2010..A reduced level of dimer formation then results from the reduced ratio of the p51E(L289K) to p51C(L289K) monomers... - Dynamic characterization of a DNA repair enzyme: NMR studies of [methyl-13C]methionine-labeled DNA polymerase betaBidisha Bose-Basu
Laboratory of Structural Biology, NIEHS, NIH, Research Triangle Park, North Carolina 27709, USA
Biochemistry 43:8911-22. 2004..Thus, the NMR data indicate that the nucleotide-DNA interaction appears to be essential for conformational activation... - Crystal structure of calmodulin binding domain of orai1 in complex with Ca2+ calmodulin displays a unique binding modeYanshun Liu
Laboratory of Neurobiology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
J Biol Chem 287:43030-41. 2012..We propose a two-step mechanism for CaM-dependent Orai1 inactivation initiated by binding of the C-lobe of CaM to the CMBD of one Orai1 followed by the binding of the N-lobe of CaM to the CMBD of a neighboring Orai1... - Backbone dynamics of the RNase H domain of HIV-1 reverse transcriptaseGeoffrey A Mueller
Laboratory of Structural Biology, NIEHS, National Institutes of Health, P.O. Box 12233, Research Triangle Park, North Carolina 27709, USA
Biochemistry 43:9332-42. 2004.... - NMR assignment of polymerase beta labeled with 2H, 13C, and 15N in complex with substrate DNAGeoffrey A Mueller
National Institute of Environmental Health Sciences, Research Triangle Park, NC 23455, USA
Biomol NMR Assign 1:33-5. 2007..We present here the first assignments of the full-length protein (335 residues, 39 kDa) in the presence of a double gap--double hairpin DNA (22 nucleotides, 7 kDa)... - Solution characterization of [methyl-(13)C]methionine HIV-1 reverse transcriptase by NMR spectroscopyXunhai Zheng
Laboratory of Molecular Biophysics, MR 01, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, USA
Antiviral Res 84:205-14. 2009..Conformational features that might explain the multiple peaks are discussed... - NMR assignment of protein side chains using residue-correlated labeling and NOE spectraGeoffrey A Mueller
National Institute of Environmental Health Sciences, Laboratory of Structural Biology, 111 Alexander Drive, PO Box 12233 MD MR-01, Research Triangle Park, NC 27709, USA
J Magn Reson 165:237-47. 2003..5 ns, and at 5 degrees C, corresponding to a rotational correlation time of 22 ns... - A synergistic approach to protein crystallization: combination of a fixed-arm carrier with surface entropy reductionAndrea F Moon
Laboratory of Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
Protein Sci 19:901-13. 2010..This crystallization technique represents a valuable rescue strategy for protein structure solution when conventional methods fail... - Metal and ligand binding to the HIV-RNase H active site are remotely monitored by Ile556Xunhai Zheng
Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, NC 27709, USA
Nucleic Acids Res 40:10543-53. 2012..Ordering of helix E results specifically from the interaction with the lower affinity binding to the A divalent ion site... - Phage like it HOT: solution structure of the bacteriophage P1-encoded HOT protein, a homolog of the theta subunit of E. coli DNA polymerase IIIEugene F Derose
Laboratory of Structural Biology, NIEHS, Box 12233, Research Triangle Park, NC 27709, USA
Structure 12:2221-31. 2004..As judged by CD measurements, the melting temperature of HOT was 62 degrees C, compared to 56 degrees C for theta, consistent with other data suggesting greater thermal stability of the HOT protein... - Solution structure of the RNase H domain of the HIV-1 reverse transcriptase in the presence of magnesiumKoteppa Pari
Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, P.O. Box 12233, Research Triangle Park, North Carolina 27709, USA
Biochemistry 42:639-50. 2003..The poor Mg(2+) binding and conformational flexibility of residues located near the active site indicate that substrate binding is a precondition for metal ion binding and for selecting the active site conformation of the RNase H domain... - NvAssign: protein NMR spectral assignment with NMRViewNigel I Kirby
Laboratory of Structural Biology, MR-01, National Institute of Environmental Health Sciences, National Institutes of Health, Box 12233, Research Triangle Park, NC 27709, USA
Bioinformatics 20:1201-3. 2004..The spectral analysis program NMRView is now able to read the output of these programs for simplified analysis and verification. AVAILABILITY: NvAssign is available from http://dir.niehs.nih.gov/dirnmr/nvassign.. - The nuclease A inhibitor represents a new variation of the rare PR-1 foldThomas W Kirby
National Institute of Environmental Health Sciences, P.O. Box 12233, MD MR-01, Research Triangle Park, NC 27709, USA
J Mol Biol 320:771-82. 2002..This hypothesis is supported by data that show that when NuiA is bound to NucA significant changes in chemical shift occur in the extended loop between helices III and IV... - NMR studies of the interaction of a type II dihydrofolate reductase with pyridine nucleotides reveal unexpected phosphatase and reductase activityWayne H Pitcher
Laboratory of Structural Biology, MR 01, National Institute of Environmental Health Sciences, National Institutes of Health, Box 12233, Research Triangle Park, North Carolina 27709, USA
Biochemistry 42:11150-60. 2003..Analysis of DHFR backbone dynamics revealed little change upon binding of NADP(+). These additional catalytic activities and dynamic behavior are in marked contrast to those of type I DHFR... - (1)H, (13)C, and (15)N NMR assignments for the Bacillus subtilis yndB START domainKelly A Mercier
Department of Chemistry, University of Nebraska Lincoln, Lincoln, NE 68588 0304, USA
Biomol NMR Assign 3:191-4. 2009..Here we report the near complete (1)H, (15)N and (13)C backbone and side chain NMR resonance assignments for the Bacillus subtilis START domain protein yndB... - NMR structure of AbhN and comparison with AbrBN: FIRST insights into the DNA binding promiscuity and specificity of AbrB-like transition state regulator proteinsBenjamin G Bobay
Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, 27695, USA
J Biol Chem 281:21399-409. 2006..The results of accompanying in vitro DNA-binding studies serve to highlight binding differences between the two proteins... - The focal adhesion targeting domain of focal adhesion kinase contains a hinge region that modulates tyrosine 926 phosphorylationKirk C Prutzman
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 USA
Structure 12:881-91. 2004..We have also identified a mutant that dimerizes approximately 8 fold more than WT FAT domain and exhibits increased phosphorylation of tyrosine 926 both in vitro and in vivo... - The N-terminal domain of the Drosophila histone mRNA binding protein, SLBP, is intrinsically disordered with nascent helical structureRoopa Thapar
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
Biochemistry 43:9390-400. 2004..The implications of this unfolded state for the function of dSLBP in regulating histone metabolism are discussed...