James B Mitchell

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Guggulsterone-mediated enhancement of radiosensitivity in human tumor cell lines
    Rajani Choudhuri
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute Bethesda, MD, USA
    Front Oncol 1:19. 2011
  2. pmc The relationship between tissue oxygenation and redox status using magnetic resonance imaging
    Fuminori Hyodo
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, USA
    Int J Oncol 41:2103-8. 2012
  3. pmc The antioxidant tempol reduces carcinogenesis and enhances survival in mice when administered after nonlethal total body radiation
    James B Mitchell
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Cancer Res 72:4846-55. 2012
  4. pmc Oral pirfenidone in patients with chronic fibrosis resulting from radiotherapy: a pilot study
    Nicole L Simone
    Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 10 CRC, Room B2 3561, Bethesda, Maryland 20892, USA
    Radiat Oncol 2:19. 2007
  5. pmc In vitro and in vivo radiation sensitization of human tumor cells by a novel checkpoint kinase inhibitor, AZD7762
    James B Mitchell
    Radiation Biology and Radiation Oncology Branches, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Clin Cancer Res 16:2076-84. 2010
  6. ncbi request reprint Radiation, radicals, and images
    J B Mitchell
    Radiation Biology Branch, National Cancer Institute, Bethesda, Maryland 20892, USA
    Ann N Y Acad Sci 899:28-43. 2000
  7. ncbi request reprint A low molecular weight antioxidant decreases weight and lowers tumor incidence
    James B Mitchell
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Free Radic Biol Med 34:93-102. 2003
  8. ncbi request reprint Novel functional imaging for tissue oxygen concentration and redox status
    James B Mitchell
    Radiation Biology Branch, National Cancer Institute NIH, Bethesda, MD 20892, USA
    J Nutr 134:3210S. 2004
  9. doi request reprint Dynamic monitoring of localized tumor oxygenation changes using RF pulsed electron paramagnetic resonance in conscious mice
    Shingo Matsumoto
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 1002, USA
    Magn Reson Med 59:619-25. 2008
  10. pmc Low-field magnetic resonance imaging to visualize chronic and cycling hypoxia in tumor-bearing mice
    Hironobu Yasui
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 1002, USA
    Cancer Res 70:6427-36. 2010

Collaborators

Detail Information

Publications129 found, 100 shown here

  1. pmc Guggulsterone-mediated enhancement of radiosensitivity in human tumor cell lines
    Rajani Choudhuri
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute Bethesda, MD, USA
    Front Oncol 1:19. 2011
    ..ERα down-regulation in response to GS suggests that it can be of potential use in the treatment of estrogen positive tumors that are resistant to tamoxifen...
  2. pmc The relationship between tissue oxygenation and redox status using magnetic resonance imaging
    Fuminori Hyodo
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, USA
    Int J Oncol 41:2103-8. 2012
    ..The results show that rapid Tempol reduction correlates with decreased tumor oxygenation, and that the Tempol decay rate constant may be a surrogate marker for tumor hypoxia...
  3. pmc The antioxidant tempol reduces carcinogenesis and enhances survival in mice when administered after nonlethal total body radiation
    James B Mitchell
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Cancer Res 72:4846-55. 2012
    ..Tempol may also find applications to reduce the risk of cancers in populations exposed to nonlethal radiation due to nuclear accidents or terrorist attacks...
  4. pmc Oral pirfenidone in patients with chronic fibrosis resulting from radiotherapy: a pilot study
    Nicole L Simone
    Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 10 CRC, Room B2 3561, Bethesda, Maryland 20892, USA
    Radiat Oncol 2:19. 2007
    ..The goal of this pilot study is to determine if Pirfenidone, a novel regulator of cytokine gene expression, has the potential to ameliorate established radiation-induced fibrosis...
  5. pmc In vitro and in vivo radiation sensitization of human tumor cells by a novel checkpoint kinase inhibitor, AZD7762
    James B Mitchell
    Radiation Biology and Radiation Oncology Branches, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Clin Cancer Res 16:2076-84. 2010
    ..A novel checkpoint kinase 1/2 inhibitor, AZD7762, was evaluated for potential enhancement of radiosensitivity for human tumor cells in vitro and in vivo xenografts...
  6. ncbi request reprint Radiation, radicals, and images
    J B Mitchell
    Radiation Biology Branch, National Cancer Institute, Bethesda, Maryland 20892, USA
    Ann N Y Acad Sci 899:28-43. 2000
    ....
  7. ncbi request reprint A low molecular weight antioxidant decreases weight and lowers tumor incidence
    James B Mitchell
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Free Radic Biol Med 34:93-102. 2003
    ..As a class, nitroxides may provide overall health benefits by contributing to decreased obesity and tumor incidence...
  8. ncbi request reprint Novel functional imaging for tissue oxygen concentration and redox status
    James B Mitchell
    Radiation Biology Branch, National Cancer Institute NIH, Bethesda, MD 20892, USA
    J Nutr 134:3210S. 2004
  9. doi request reprint Dynamic monitoring of localized tumor oxygenation changes using RF pulsed electron paramagnetic resonance in conscious mice
    Shingo Matsumoto
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 1002, USA
    Magn Reson Med 59:619-25. 2008
    ..9 +/- 0.5 mmHg for tumor, 8.8 +/- 1.2 mmHg for muscle). These results support the advantage of pulsed EPR in examining pO2 in conscious animals with LiPc chronically implanted in predetermined regions...
  10. pmc Low-field magnetic resonance imaging to visualize chronic and cycling hypoxia in tumor-bearing mice
    Hironobu Yasui
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 1002, USA
    Cancer Res 70:6427-36. 2010
    ..This EPRI technique, combined with MRI, may offer a powerful clinical tool to noninvasively detect variable oxygenation in tumors...
  11. ncbi request reprint Influence of proton T1 on oxymetry using Overhauser enhanced magnetic resonance imaging
    Shingo Matsumoto
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA
    Magn Reson Med 54:213-7. 2005
    ..2 approximately 1.6 s). Computation of pO2 images using pixel-wise T1 values promises oximetry with minimal artifacts by OMRI...
  12. pmc Monitoring redox-sensitive paramagnetic contrast agent by EPRI, OMRI and MRI
    Fuminori Hyodo
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Building 10, Room B3B69, NIH, Bethesda, MD 20892 1002, USA
    J Magn Reson 190:105-12. 2008
    ..Therefore, a combination of MRI with nitroxides as metabolically responsive contrast agents can be a useful technique for the in vivo imaging probing tissue redox status...
  13. ncbi request reprint Gene expression profiling of breast, prostate, and glioma cells following single versus fractionated doses of radiation
    Mong Hsun Tsai
    Radiation Biology, Center for Cancer Research, National Cancer Institute NIH, Bethesda, MD 20892, USA
    Cancer Res 67:3845-52. 2007
    ....
  14. ncbi request reprint Differential protection by nitroxides and hydroxylamines to radiation-induced and metal ion-catalyzed oxidative damage
    Sandhya Xavier
    Radiation Biology Branch, National Cancer Institute, Bldg 10, Room B3 B69, 9000 Rockville Pike, Bethesda, MD 20892 1002, USA
    Biochim Biophys Acta 1573:109-20. 2002
    ....
  15. ncbi request reprint Radiation-induced changes in gene-expression profiles for the SCC VII tumor cells grown in vitro and in vivo
    John A Cook
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Antioxid Redox Signal 8:1263-72. 2006
    ....
  16. pmc Pulsed EPR imaging of nitroxides in mice
    Fuminori Hyodo
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 1002, USA
    J Magn Reson 197:181-5. 2009
    ..This study, for the first time shows the capability to image a cell-permeable nitroxide in mice using pulsed EPR in the SPI modality...
  17. pmc Antiangiogenic agent sunitinib transiently increases tumor oxygenation and suppresses cycling hypoxia
    Shingo Matsumoto
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Cancer Res 71:6350-9. 2011
    ..Overall, the findings suggest that longitudinal and noninvasive monitoring of tumor pO(2) makes it possible to identify a window of vascular renormalization to maximize the effects of combination therapy with antiangiogenic drugs...
  18. pmc Longitudinal imaging studies of tumor microenvironment in mice treated with the mTOR inhibitor rapamycin
    Keita Saito
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 7:e49456. 2012
    ..Improved oxygenation by rapamycin treatment provides a temporal window for anti-cancer therapies to realize enhanced response to radiotherapy...
  19. pmc Assessment of tissue redox status using metabolic responsive contrast agents and magnetic resonance imaging
    Fuminori Hyodo
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, USA
    J Pharm Pharmacol 60:1049-60. 2008
    ....
  20. ncbi request reprint Electron paramagnetic resonance imaging of tumor pO₂
    Murali C Krishna
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institute of Health, Bethesda, Maryland 20892, USA
    Radiat Res 177:376-86. 2012
    ..In this article, the principles of pO₂ imaging with EPR and some relevant examples of tumor imaging are reviewed...
  21. ncbi request reprint Electron paramagnetic resonance imaging of tumor hypoxia: enhanced spatial and temporal resolution for in vivo pO2 determination
    Ken Ichiro Matsumoto
    Radiation Biology Branch, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892 1002, USA
    Magn Reson Med 55:1157-63. 2006
    ..12% oxygen in gas phase). This work demonstrates that in vivo oximetry can be performed with good sensitivity, accuracy, and high spatial and temporal resolution...
  22. ncbi request reprint Probing the intracellular redox status of tumors with magnetic resonance imaging and redox-sensitive contrast agents
    Fuminori Hyodo
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    Cancer Res 66:9921-8. 2006
    ....
  23. pmc Cellular stress induced alterations in microRNA let-7a and let-7b expression are dependent on p53
    Anthony D Saleh
    Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 6:e24429. 2011
    ....
  24. pmc Radioprotectors and mitigators of radiation-induced normal tissue injury
    Deborah Citrin
    Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Oncologist 15:360-71. 2010
    ..Few agents are approved for clinical use, but many new compounds show promising results in preclinical testing...
  25. ncbi request reprint Intracellular hypoxia of tumor tissue estimated by noninvasive electron paramagnetic resonance oximetry technique using paramagnetic probes
    Atsuko Matsumoto
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 1002, USA
    Biol Pharm Bull 34:142-5. 2011
    ..The SCC tumor showed intracellular hypoxia even though the extracellular pO(2) is similar to normal tissue in the peripheral region...
  26. ncbi request reprint High-resolution mapping of tumor redox status by magnetic resonance imaging using nitroxides as redox-sensitive contrast agents
    Ken Ichiro Matsumoto
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, and Laboratory of Functional and Molecular Imaging, National Institutes of Neurological Disorders and Stroke, NIH, Bethesda, Maryland, USA
    Clin Cancer Res 12:2455-62. 2006
    ..The present study sought to determine the efficacy of nitroxide radioprotectors as functional image contrast agents...
  27. ncbi request reprint Factors influencing nitroxide reduction and cytotoxicity in vitro
    Yuval Samuni
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Antioxid Redox Signal 6:587-95. 2004
    ....
  28. ncbi request reprint Strategies for improved temporal and spectral resolution in in vivo oximetric imaging using time-domain EPR
    Nallathamby Devasahayam
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA
    Magn Reson Med 57:776-83. 2007
    ....
  29. ncbi request reprint Differential radiation protection of salivary glands versus tumor by Tempol with accompanying tissue assessment of Tempol by magnetic resonance imaging
    Ana P Cotrim
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, NIH, Bethesda, Maryland 20892, USA
    Clin Cancer Res 13:4928-33. 2007
    ..Mechanistic information was explored by monitoring the presence and bioreduction of Tempol in both tissues noninvasively by magnetic resonance imaging (MRI)...
  30. pmc Transient decrease in tumor oxygenation after intravenous administration of pyruvate
    Keita Saito
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 1002, USA
    Magn Reson Med 67:801-7. 2012
    ..These results suggest importance of taking into account the transient decrease in tumor pO(2) after pyruvate injection in hyperpolarized (13) C MRI, because tumor oxygen status is an important factor in determining outcomes of therapies...
  31. pmc Reporting of quantitative oxygen mapping in EPR imaging
    Sankaran Subramanian
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    J Magn Reson 214:244-51. 2012
    ..Therefore, specification of intrinsic resolution and pO(2) uncertainties are necessary to interpret digitally processed pO(2) illustrations...
  32. pmc The effects of resveratrol and selected metabolites on the radiation and antioxidant response
    Kristin M Fabre
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, USA
    Cancer Biol Ther 12:915-23. 2011
    ..These cytotoxic effects were abolished when HBED, a metal chelator, was added. Taken together RSV and many of its metabolic derivatives are not effective as chemical radioprotectors and should not be considered for clinical use...
  33. ncbi request reprint Estimation of tumor microvessel density by MRI using a blood pool contrast agent
    Fuminori Hyodo
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Int J Oncol 35:797-804. 2009
    ....
  34. pmc Low-field paramagnetic resonance imaging of tumor oxygenation and glycolytic activity in mice
    Shingo Matsumoto
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 1002, USA
    J Clin Invest 118:1965-73. 2008
    ..In addition, this method may also be useful for studying the relationship between pO(2) status and tumor-specific phenotypes such as aerobic glycolysis...
  35. pmc Simultaneous imaging of tumor oxygenation and microvascular permeability using Overhauser enhanced MRI
    Shingo Matsumoto
    Radiation Biology Branch, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:17898-903. 2009
    ..This dual imaging technique may be useful for the longitudinal assessment of changes in tumor vascular function and oxygenation in response to chemotherapy, radiotherapy, or antiangiogenic treatment...
  36. ncbi request reprint Application of a macromolecular contrast agent for detection of alterations of tumor vessel permeability induced by radiation
    Hisataka Kobayashi
    Metabolism Branch, Radiation Biology Branch, and Radiation Oncology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 1002, USA
    Clin Cancer Res 10:7712-20. 2004
    ..Such information might be applied to optimize the efficacy of subsequent or concurrent therapies including radiolabeled antibodies or other anticancer agents in combination with external beam therapies...
  37. ncbi request reprint Radio frequency continuous-wave and time-domain EPR imaging and Overhauser-enhanced magnetic resonance imaging of small animals: instrumental developments and comparison of relative merits for functional imaging
    Sankaran Subramanian
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    NMR Biomed 17:263-94. 2004
    ....
  38. pmc PI3K/mTOR inhibitor PF-04691502 antitumor activity is enhanced with induction of wild-type TP53 in human xenograft and murine knockout models of head and neck cancer
    Amanda Herzog
    Tumor Biology Section, Head and Neck Surgery Branch, National Institute on Deafness and Other Communication Disorders, NIH, Bethesda, Maryland 20892, USA
    Clin Cancer Res 19:3808-19. 2013
    ..However, little is known about how these alterations affect response to PI3K/mTOR-targeted agents...
  39. pmc EPR oxygen imaging and hyperpolarized 13C MRI of pyruvate metabolism as noninvasive biomarkers of tumor treatment response to a glycolysis inhibitor 3-bromopyruvate
    Shingo Matsumoto
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 1002, USA
    Magn Reson Med 69:1443-50. 2013
    ..These results emphasize the importance of noninvasive imaging biomarkers to confirm the action of hypoxia-activated drugs...
  40. ncbi request reprint Oxidative stress, redox, and the tumor microenvironment
    John A Cook
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Semin Radiat Oncol 14:259-66. 2004
    ..With respect to the radiation treatment of cancer, components of the cellular redox armamentarium may be targeted to enhance cell killing in the case of tumors and/or protection in the case of normal tissues...
  41. ncbi request reprint Spatially resolved biologic information from in vivo EPRI, OMRI, and MRI
    Ken Ichiro Matsumoto
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    Antioxid Redox Signal 9:1125-41. 2007
    ..For this review, the EPRI, OMRI, and MRI applications for the study biological systems were evaluated for researchers to apply the method of choice and the mode of measurements to specific experimental systems...
  42. pmc Evaluation of the fullerene compound DF-1 as a radiation protector
    Aaron P Brown
    Radiation Oncology Branch, National Cancer Institute, Building 10 CRC B2 3500, Bethesda, MD 20892, USA
    Radiat Oncol 5:34. 2010
    ..The purpose of this study was to evaluate the radioprotective effects of DF-1 in a murine model of lethal total body irradiation and to assess for selective radioprotection of normal cells versus tumor cells...
  43. ncbi request reprint Absolute oxygen tension (pO(2)) in murine fatty and muscle tissue as determined by EPR
    Atsuko Matsumoto
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892 1002, USA
    Magn Reson Med 54:1530-5. 2005
    ....
  44. pmc Interference with transforming growth factor-beta/ Smad3 signaling results in accelerated healing of wounds in previously irradiated skin
    Kathleen C Flanders
    Laboratory of Cell Regulation and Carcinogenesis and the Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 21201, USA
    Am J Pathol 163:2247-57. 2003
    ..Overall, the data suggest that attenuation of Smad3 signaling might improve the healing of wounds in previously irradiated skin commensurate with an inhibition of fibrosis...
  45. pmc The inhibitors of histone deacetylase suberoylanilide hydroxamate and trichostatin A release nitric oxide upon oxidation
    Yuval Samuni
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 47:419-23. 2009
    ..These studies provide evidence for the release of NO by hydroxamate-containing HDAC inhibitors and their antioxidant effects. Such roles may be an added advantage of this class of HDAC agents used for epigenetic therapies in cancer...
  46. ncbi request reprint Pharmacokinetics of a triarylmethyl-type paramagnetic spin probe used in EPR oximetry
    Ken Ichiro Matsumoto
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892 1002, USA
    Magn Reson Med 52:885-92. 2004
    ..033-0.040 min(-1). Continuous infusion following a bolus injection of the probe was found to be effective to obtain stable plasma concentration as well as image intensity to permit reliable pO2 estimates...
  47. pmc Prevention of radiation-induced salivary hypofunction following hKGF gene delivery to murine submandibular glands
    Changyu Zheng
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892 1190, USA
    Clin Cancer Res 17:2842-51. 2011
    ..We evaluated the effect of human keratinocyte growth factor (hKGF) gene transfer to murine salivary glands on the prevention of radiation-induced salivary hypofunction...
  48. ncbi request reprint An application system for automation of constant-time radio frequency electron paramagnetic resonance imaging
    Andrew G Taube
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Comput Methods Programs Biomed 72:127-38. 2003
    ..Although the application system has been developed for the specific purpose of EPR imaging, it can easily be adapted to other areas such as magnetic resonance microscopy as well...
  49. pmc Magnetic resonance imaging of organic contrast agents in mice: capturing the whole-body redox landscape
    Ryan M Davis
    Radiation Biology Branch, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 50:459-68. 2011
    ..For the purpose of redox imaging, 3-CP was shown to be an optimal choice based on the achievable concentrations and bioreduction observed in vivo...
  50. pmc The influence of tumor oxygenation on (18)F-FDG (fluorine-18 deoxyglucose) uptake: a mouse study using positron emission tomography (PET)
    Linda W Chan
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA
    Radiat Oncol 1:3. 2006
    ..This study investigated whether changing a tumor's oxygenation would alter tumor metabolism, and thus uptake of (18)F-FDG (fluorine-18 deoxyglucose), a marker for glucose metabolism using positron emission tomography (PET)...
  51. ncbi request reprint Therapeutic and clinical applications of nitroxide compounds
    Benjamin P Soule
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    Antioxid Redox Signal 9:1731-43. 2007
    ..The therapeutic and research uses of nitroxides are reviewed here, with a focus on the progress from initial development to modern, state-of-the art trials...
  52. doi request reprint Echo-based Single Point Imaging (ESPI): a novel pulsed EPR imaging modality for high spatial resolution and quantitative oximetry
    Sankaran Subramanian
    Radiation Biology Branch, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, MD 20892, USA
    J Magn Reson 218:105-14. 2012
    ..The Fourier reconstruction, line width independent resolution and the true T(2)-weighting make this novel procedure very attractive for in vivo EPR imaging of tissue oxygen quantitatively...
  53. ncbi request reprint Noninvasive in vivo oximetric imaging by radiofrequency FT EPR
    Sankaran Subramanian
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892 1002, USA
    Magn Reson Med 47:1001-8. 2002
    ..Thus, from a single spatial image data set, pO(2) measurements can be done noninvasively by relaxo-oximetry, and 3D imaging can be performed in less than 3 min...
  54. ncbi request reprint Continuous wave EPR oximetric imaging at 300 MHz using radiofrequency power saturation effects
    Yukihiro Hama
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892 1002, USA
    Antioxid Redox Signal 9:1709-16. 2007
    ..In vivo power saturation oximetric imaging in a tumor bearing mouse clearly depicted the hypoxic foci within the tumor...
  55. pmc Loratadine dysregulates cell cycle progression and enhances the effect of radiation in human tumor cell lines
    Benjamin P Soule
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Radiat Oncol 5:8. 2010
    ..Since this is a radiation sensitive phase of the cell cycle, we sought to determine if loratadine modifies radiosensitivity in several human tumor cell lines with emphasis on human colon carcinoma (HT29)...
  56. doi request reprint PX-478, an inhibitor of hypoxia-inducible factor-1alpha, enhances radiosensitivity of prostate carcinoma cells
    Sanjeewani T Palayoor
    Radiation Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
    Int J Cancer 123:2430-7. 2008
    ..Although the precise mechanism of enhancement of radiosensitivity remains to be identified, this study suggests a potential role for PX-478 as a clinical radiation enhancer...
  57. pmc Halofuginone enhances the radiation sensitivity of human tumor cell lines
    John A Cook
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892 1002, USA
    Cancer Lett 289:119-26. 2010
    ..These data further support the goal of manipulating the TGF-beta pathway to achieve a positive increase in the therapeutic gain in clinical radiotherapy...
  58. ncbi request reprint The effects of antioxidants on radiation-induced apoptosis pathways in TK6 cells
    Ayelet M Samuni
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 1002, USA
    Free Radic Biol Med 37:1648-55. 2004
    ..Collectively, the results question the validity of using selected apoptosis pathway members as sole indicators of cytotoxicity...
  59. pmc Overhauser enhanced magnetic resonance imaging for tumor oximetry: coregistration of tumor anatomy and tissue oxygen concentration
    Murali C Krishna
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:2216-21. 2002
    ....
  60. pmc mTOR inhibition prevents epithelial stem cell senescence and protects from radiation-induced mucositis
    Ramiro Iglesias-Bartolomé
    Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20852, USA
    Cell Stem Cell 11:401-14. 2012
    ..mTOR inhibition also protects from the loss of proliferative basal epithelial stem cells upon ionizing radiation in vivo, thereby preserving the integrity of the oral mucosa and protecting from radiation-induced mucositis...
  61. ncbi request reprint Abnormal gene expression profiles in unaffected parents of patients with hereditary-type retinoblastoma
    Eric Y Chuang
    Radiation Biology and Oncology Branches, Center for Cancer Research, National Cancer Institute NIH, Bethesda, MD, USA
    Cancer Res 66:3428-33. 2006
    ..Further study of this phenomenon may offer a new insight into the genetic mechanisms of Rb and perhaps more broadly in cancer biology...
  62. ncbi request reprint Distinct effects on gene expression of chemical and genetic manipulation of the cancer epigenome revealed by a multimodality approach
    David Gius
    Radiation Oncology Branch, Radiation Oncology Sciences Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Cancer Cell 6:361-71. 2004
    ..In addition, a 75 kb cluster of metallothionein genes was coordinately regulated...
  63. ncbi request reprint Estimation of free radical formation by beta-ray irradiation in rat liver
    Ken Ichiro Matsumoto
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892 1002, USA
    J Biochem Biophys Methods 63:79-90. 2005
    ..This study shows possible evidence of reactive oxygen species formation by low-dose beta-ray irradiation in a living animal...
  64. ncbi request reprint Influence of body temperature on the BOLD effect in murine SCC tumors
    Koen Reijnders
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    Magn Reson Med 51:389-93. 2004
    ..The findings of the study stress the importance of maintaining normal core temperature when assessing tumor oxygen status using functional imaging modalities or oxygen-sensitive electrodes...
  65. ncbi request reprint MR assessment of changes of tumor in response to hyperbaric oxygen treatment
    Ken Ichiro Matsumoto
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892 1002, USA
    Magn Reson Med 56:240-6. 2006
    ....
  66. ncbi request reprint Kinetics of tempol for prevention of xerostomia following head and neck irradiation in a mouse model
    Ana P Cotrim
    Gene Therapy and Therapeutics Branch and Biostatistics Core, Division of Clinical Research and Health Promotion, National Institute of Dental and Craniofacial Research, NIH, Bethesda, Maryland 20892, USA
    Clin Cancer Res 11:7564-8. 2005
    ..The purpose of this study was to examine pharmacokinetic characteristics of the stable nitroxide 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (tempol) with respect to radioprotection of the salivary glands...
  67. pmc A novel nitroxide is an effective brain redox imaging contrast agent and in vivo radioprotector
    Ryan M Davis
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Free Radic Biol Med 51:780-90. 2011
    ..The data in this report suggest that 23c is currently the most potent known nitroxide radioprotector, and that it may also be useful as a contrast agent for functional imaging...
  68. pmc Prevention of radiation-induced oral mucositis after adenoviral vector-mediated transfer of the keratinocyte growth factor cDNA to mouse submandibular glands
    Changyu Zheng
    Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Cancer Institute, Bethesda, Maryland, USA
    Clin Cancer Res 15:4641-8. 2009
    ..The study aims to evaluate if human keratinocyte growth factor (hKGF), secreted after transduction of murine salivary glands with adenoviral vectors, can prevent oral mucositis resulting from radiation...
  69. pmc Ionizing radiation-induced oxidative stress alters miRNA expression
    Nicole L Simone
    Radiation Oncology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, USA
    PLoS ONE 4:e6377. 2009
    ..Alterations in miRNA expression may occur following exposure to several stress-inducing anticancer agents including ionizing radiation, etoposide, and hydrogen peroxide (H(2)O(2))...
  70. ncbi request reprint Amelioration of radiation-induced fibrosis: inhibition of transforming growth factor-beta signaling by halofuginone
    Sandhya Xavier
    Radiation Biology Branch, Nationa Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:15167-76. 2004
    ..The results detail the molecular effects of halofuginone on the TGF-beta signal pathway and show that halofuginone may lessen radiation-induced fibrosis in humans...
  71. pmc Absence of Smad3 confers radioprotection through modulation of ERK-MAPK in primary dermal fibroblasts
    Praveen R Arany
    Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Dermatol Sci 48:35-42. 2007
    ....
  72. ncbi request reprint Effects of hypoxia on radiation-responsive stress-activated protein kinase, p53, and caspase 3 signals in TK6 human lymphoblastoid cells
    Ayelet M Samuni
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Cancer Res 65:579-86. 2005
    ..These results suggest that radiation-induced apoptosis under hypoxia occurs independent of phospho-SAPK and caspase 3, and the p53 response is an obligatory apoptotic signal in TK6 cells...
  73. ncbi request reprint Application of continuous-wave EPR spectral-spatial image reconstruction techniques for in vivo oxymetry: comparison of projection reconstruction and constant-time modalities
    Ken Ichiro Matsumoto
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Magn Reson Med 50:865-74. 2003
    ..CTSSI promises to be a more suitable method for quantitative in vivo oxymetric studies using radiofrequency EPR imaging (EPRI)...
  74. ncbi request reprint The influence of tumor oxygenation on hypoxia imaging in murine squamous cell carcinoma using [64Cu]Cu-ATSM or [18F]Fluoromisonidazole positron emission tomography
    Ken Ichiro Matsumoto
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Int J Oncol 30:873-81. 2007
    ..While the mechanism of nitroimidazole binding to hypoxic cells has been extensively studied, the avid binding of Cu-ATSM to tumors may involve other mechanisms independent of hypoxia that warrant further study...
  75. ncbi request reprint Evaluation of radiation-induced oral mucositis by optical coherence tomography
    Thierry M Muanza
    Radiation Oncology Branch, National Cancer Institute, Bethesda, Maryland 20892, USA
    Clin Cancer Res 11:5121-7. 2005
    ..Optical coherence tomography (OCT) imaging was evaluated to determine if radiation-induced mucosal damage could be noninvasively monitored in real time and correlated with histopathologic findings...
  76. pmc Halofuginone mediated protection against radiation-induced leg contracture
    Hisanari Ishii
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892 1002, USA
    Int J Oncol 35:315-9. 2009
    ..The latter, TSP1, a co-activator of TGF-beta may serve as a suitable biomarker for monitoring the efficacy of halofuginone should it be evaluated in a clinical setting for protection against radiation-induced fibrosis...
  77. ncbi request reprint Fluorine electron double resonance imaging for 19F MRI in low magnetic fields
    Ramachandran Murugesan
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Magn Reson Med 48:523-9. 2002
    ..This feasibility of coregistration of proton-based anatomical PEDRI image with physiological FEDRI image offers good potential for studying fluorine-containing tracers...
  78. ncbi request reprint Mechanisms underlying the cytotoxic effects of Tachpyr--a novel metal chelator
    Ayelet M Samuni
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892 1002, USA
    Biochim Biophys Acta 1571:211-8. 2002
    ..Therefore, in addition to iron depletion, low-level oxidative stress, which in part occurs because of redox cycling of the coordinated iron ion, may contribute to the cytotoxic effects of Tachpyr...
  79. ncbi request reprint Nitroxides as antioxidants: Tempol protects against EO9 cytotoxicity
    Ayelet M Samuni
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892 1002, USA
    Mol Cell Biochem 234:327-33. 2002
    ..or detoxification of O2.- and H2O2...
  80. pmc Semiquinone radical intermediate in catecholic estrogen-mediated cytotoxicity and mutagenesis: chemoprevention strategies with antioxidants
    Ayelet M Samuni
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 10, Room B3 B69, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 100:5390-5. 2003
    ....
  81. ncbi request reprint The stable nitroxide tempol facilitates salivary gland protection during head and neck irradiation in a mouse model
    Joseph M Vitolo
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, NIH, Bethesda, Maryland 20892, USA
    Clin Cancer Res 10:1807-12. 2004
    ..The purpose of this study was to test the ability of the stable-free radical Tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl), an established radioprotector, to prevent radiation-induced salivary hypofunction in mice...
  82. ncbi request reprint Effects of gamma radiation on FcepsilonRI and TLR-mediated mast cell activation
    Benjamin P Soule
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
    J Immunol 179:3276-86. 2007
    ..Mast cells are thus resistant to the cytotoxic effects and alterations in function after irradiation and, despite a transient inhibition, ultimately respond to innate and acquired immune activation signals...
  83. ncbi request reprint Gene expression after treatment with hydrogen peroxide, menadione, or t-butyl hydroperoxide in breast cancer cells
    Yao Yu Eric Chuang
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Cancer Res 62:6246-54. 2002
    ..Analyzing the three oxidants simultaneously by template cluster analysis identified genes that heretofore have not been associated with oxidative stress...
  84. pmc Absence of Smad3 induces neutrophil migration after cutaneous irradiation: possible contribution to subsequent radioprotection
    Kathleen C Flanders
    Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 4255, USA
    Am J Pathol 173:68-76. 2008
    ..Thus, early responses, including enhanced neutrophil influx, appear to contribute to subsequent cutaneous radioprotection...
  85. ncbi request reprint Nitroxides as cancer imaging agents
    Ryan M Davis
    Radiation Biology Branch, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, USA
    Anticancer Agents Med Chem 11:347-58. 2011
    ..The potential therapeutic applications and bio-reduction mechanisms are discussed in the context of their relevance to oncology...
  86. ncbi request reprint Prevention of irradiation-induced salivary hypofunction by microvessel protection in mouse salivary glands
    Ana P Cotrim
    Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, Bethesda, Maryland 20892 1190, USA
    Mol Ther 15:2101-6. 2007
    ..Furthermore, our results suggest that a local transient treatment directed at protecting SG endothelial cells may be beneficial for patients undergoing IR for head and neck cancer...
  87. pmc The chemistry and biology of nitroxide compounds
    Benjamin P Soule
    Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Free Radic Biol Med 42:1632-50. 2007
    ..The therapeutic and research uses of nitroxide compounds are reviewed here with a focus on the progress from initial development to modern trials...
  88. ncbi request reprint Further evidence for distinct reactive intermediates from nitroxyl and peroxynitrite: effects of buffer composition on the chemistry of Angeli's salt and synthetic peroxynitrite
    Katrina M Miranda
    Radiation Biology Branch, National Cancer Institute NIH, Building 10, Room B3 B69, Bethesda, MD 20892, USA
    Arch Biochem Biophys 401:134-44. 2002
    ..This study emphasizes the importance of buffer composition on chemical outcome and thus on interpretation and provides further evidence that ONOO(-) is not an intermediate formed between the reaction of O(2) and HNO produced by AS...
  89. ncbi request reprint EPR imaging of vascular changes in oxygen in response to carbogen breathing
    William Stein
    Radiation Biology Branch, National Cancer Institute, Bethesda, Maryland 20892, USA
    Adv Exp Med Biol 510:231-6. 2003
  90. ncbi request reprint Nitric oxide and cancer: an introduction
    David A Wink
    Tumor Biology Section, Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Free Radic Biol Med 34:951-4. 2003
  91. ncbi request reprint Molecular and cellular biology of moderate-dose (1-10 Gy) radiation and potential mechanisms of radiation protection: report of a workshop at Bethesda, Maryland, December 17-18, 2001
    C Norman Coleman
    Radiation Oncology Sciences Program, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    Radiat Res 159:812-34. 2003
    ..Communicate knowledge of the effects of radiation exposure to the general public and to investigators, policy makers and agencies involved in response to nuclear accidents/events and protection/treatment of the general public...
  92. ncbi request reprint Expression of human MutT homologue (hMTH1) protein in primary non-small-cell lung carcinomas and histologically normal surrounding tissue
    Christopher H Kennedy
    Cancer Prevention Fellowship Program, Office of Preventive Oncology, Division of Cancer Prevention, National Cancer Institute NIH, 10 Center Drive, Building 10, Bethesda, MD 20892 1002, USA
    Free Radic Biol Med 34:1447-57. 2003
    ..The results of this study indicate that hMTH1 protein may be a potential marker for the detection of persistent oxidative stress in lung cancer...
  93. ncbi request reprint Protection against Adriamycin cytotoxicity and inhibition of DNA topoisomerase II activity by 3,4-dihydroxybenzoic acid
    William G De Graff
    Radiation Biology Branch, National Cancer Institute NIH, Building 10, Room B3 B69, 10 Center Drive, Bethesda, MD 20892, USA
    Int J Oncol 23:159-63. 2003
    ..These data identify DHB as a non-toxic inhibitor of DNA topoisomerase II and suggest that much of the cytotoxicity of ADR in actively growing V79 cells is due to mechanisms other than redox cycling by the semiquinone...
  94. ncbi request reprint Geldanamycin and 17-allylamino-17-demethoxygeldanamycin potentiate the in vitro and in vivo radiation response of cervical tumor cells via the heat shock protein 90-mediated intracellular signaling and cytotoxicity
    Kheem S Bisht
    Radiation Oncology Branch and Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 1002, USA
    Cancer Res 63:8984-95. 2003
    ..This work shows that altered HSP90 function induces significant tumor cytotoxicity and radiosensitization, suggesting a potential therapeutic utility...
  95. pmc Mice lacking Smad3 are protected against cutaneous injury induced by ionizing radiation
    Kathleen C Flanders
    Laboratory of Cell Regulation and Carcinogenesis and Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 5055, USA
    Am J Pathol 160:1057-68. 2002
    ..These results suggest that inhibition of Smad3 might decrease tissue damage and reduce fibrosis after exposure to ionizing irradiation...
  96. ncbi request reprint Kinetics and mechanism of hydroxyl radical and OH-adduct radical reactions with nitroxides and with their hydroxylamines
    Amram Samuni
    Radiation Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Am Chem Soc 124:8719-24. 2002
    ..Instead, the radioprotective activity of nitroxides, but not of hydroxylamines, can be partially attributed to their ability to detoxify OH-derived secondary radicals...
  97. ncbi request reprint Inhibition of nuclear factor-kappaB and target genes during combined therapy with proteasome inhibitor bortezomib and reirradiation in patients with recurrent head-and-neck squamous cell carcinoma
    Carter Van Waes
    Head and Neck Surgery Branch, National Institute on Deafness and Other Communication Disorders, Bethesda, MD 20892, USA
    Int J Radiat Oncol Biol Phys 63:1400-12. 2005
    ....
  98. ncbi request reprint Superoxide fluxes limit nitric oxide-induced signaling
    Douglas D Thomas
    Tumor Biology Section, Radiation Biology Branch, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 281:25984-93. 2006
    ..We conclude that the reaction of O2- with NO is an important regulatory mechanism, which modulates signaling pathways by limiting steady-state levels of NO and preventing H2O2 formation from O2-...
  99. pmc Pharmacologic ascorbic acid concentrations selectively kill cancer cells: action as a pro-drug to deliver hydrogen peroxide to tissues
    Qi Chen
    Molecular and Clinical Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:13604-9. 2005
    ..These findings give plausibility to i.v. ascorbic acid in cancer treatment, and have unexpected implications for treatment of infections where H(2)O(2) may be beneficial...
  100. ncbi request reprint Transfer of the AQP1 cDNA for the correction of radiation-induced salivary hypofunction
    Bruce J Baum
    Gene Therapy and Therapeutics Branch, Bethesda, MD 20892 1190, USA
    Biochim Biophys Acta 1758:1071-7. 2006
    ..Based on these results, we are developing a clinical trial to test if the hAQP1 cDNA transfer strategy will be clinically effective in restoring salivary flow in patients with IR-induced parotid hypofunction...
  101. ncbi request reprint Superoxide modulates the oxidation and nitrosation of thiols by nitric oxide-derived reactive intermediates. Chemical aspects involved in the balance between oxidative and nitrosative stress
    D A Wink
    Tumor Biology Section, Radiation Biology Branch, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 272:11147-51. 1997
    ..The results suggest that oxidation and nitrosation of thiols by superoxide and NO are determined by their relative fluxes and may have physiological significance...