Jacqueline L S Milne

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Molecular architecture and mechanism of an icosahedral pyruvate dehydrogenase complex: a multifunctional catalytic machine
    Jacqueline L S Milne
    Laboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    EMBO J 21:5587-98. 2002
  2. pmc Molecular structure of a 9-MDa icosahedral pyruvate dehydrogenase subcomplex containing the E2 and E3 enzymes using cryoelectron microscopy
    Jacqueline L S Milne
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 281:4364-70. 2006
  3. pmc Structural mechanism of trimeric HIV-1 envelope glycoprotein activation
    Erin E H Tran
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, United States of America
    PLoS Pathog 8:e1002797. 2012
  4. pmc Three-dimensional structures of soluble CD4-bound states of trimeric simian immunodeficiency virus envelope glycoproteins determined by using cryo-electron tomography
    Tommi A White
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    J Virol 85:12114-23. 2011
  5. doi request reprint Extended polypeptide linkers establish the spatial architecture of a pyruvate dehydrogenase multienzyme complex
    Jeffrey S Lengyel
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Structure 16:93-103. 2008
  6. pmc Cryo-electron microscopy--a primer for the non-microscopist
    Jacqueline L S Milne
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    FEBS J 280:28-45. 2013
  7. ncbi request reprint Automated image acquisition and processing using a new generation of 4K x 4K CCD cameras for cryo electron microscopic studies of macromolecular assemblies
    Peijun Zhang
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Struct Biol 143:135-44. 2003
  8. ncbi request reprint Visualization of alpha-helical features in a density map constructed using 9 molecular images of the 1.8 MDa icosahedral core of pyruvate dehydrogenase
    Mario J Borgnia
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Struct Biol 147:136-45. 2004
  9. ncbi request reprint A core-weighted fitting method for docking atomic structures into low-resolution maps: application to cryo-electron microscopy
    Xiongwu Wu
    Laboratory of Biophysical Chemistry, NHLBI, National Institutes of Health, Building 50, Room 3308, 50 South Drive, Bethesda, MD 20892, USA
    J Struct Biol 141:63-76. 2003
  10. ncbi request reprint Three-dimensional electron microscopy at molecular resolution
    Sriram Subramaniam
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    Annu Rev Biophys Biomol Struct 33:141-55. 2004

Collaborators

Detail Information

Publications21

  1. pmc Molecular architecture and mechanism of an icosahedral pyruvate dehydrogenase complex: a multifunctional catalytic machine
    Jacqueline L S Milne
    Laboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    EMBO J 21:5587-98. 2002
    ....
  2. pmc Molecular structure of a 9-MDa icosahedral pyruvate dehydrogenase subcomplex containing the E2 and E3 enzymes using cryoelectron microscopy
    Jacqueline L S Milne
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 281:4364-70. 2006
    ....
  3. pmc Structural mechanism of trimeric HIV-1 envelope glycoprotein activation
    Erin E H Tran
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, United States of America
    PLoS Pathog 8:e1002797. 2012
    ..These findings suggest a new structural template for designing immunogens that can elicit antibodies targeting HIV at a vulnerable, pre-entry stage...
  4. pmc Three-dimensional structures of soluble CD4-bound states of trimeric simian immunodeficiency virus envelope glycoproteins determined by using cryo-electron tomography
    Tommi A White
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    J Virol 85:12114-23. 2011
    ..These results provide new insights into the structural diversity of SIV Env and show that there are strain-dependent variations in the orientation of sCD4 bound to trimeric SIV Env...
  5. doi request reprint Extended polypeptide linkers establish the spatial architecture of a pyruvate dehydrogenase multienzyme complex
    Jeffrey S Lengyel
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Structure 16:93-103. 2008
    ....
  6. pmc Cryo-electron microscopy--a primer for the non-microscopist
    Jacqueline L S Milne
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    FEBS J 280:28-45. 2013
    ..Finally, we include a description of how the structural information obtained by cryo-EM is deposited and archived in a publicly accessible database...
  7. ncbi request reprint Automated image acquisition and processing using a new generation of 4K x 4K CCD cameras for cryo electron microscopic studies of macromolecular assemblies
    Peijun Zhang
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Struct Biol 143:135-44. 2003
    ..8 MDa Bacillus stearothermophilus icosahedral pyruvate dehydrogenase complex, and its comparison with the previously reported atomic model for this complex obtained by X-ray crystallography...
  8. ncbi request reprint Visualization of alpha-helical features in a density map constructed using 9 molecular images of the 1.8 MDa icosahedral core of pyruvate dehydrogenase
    Mario J Borgnia
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Struct Biol 147:136-45. 2004
    ....
  9. ncbi request reprint A core-weighted fitting method for docking atomic structures into low-resolution maps: application to cryo-electron microscopy
    Xiongwu Wu
    Laboratory of Biophysical Chemistry, NHLBI, National Institutes of Health, Building 50, Room 3308, 50 South Drive, Bethesda, MD 20892, USA
    J Struct Biol 141:63-76. 2003
    ....
  10. ncbi request reprint Three-dimensional electron microscopy at molecular resolution
    Sriram Subramaniam
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    Annu Rev Biophys Biomol Struct 33:141-55. 2004
    ..The rapid pace of development in this field suggests that comprehensive descriptions of the structures of whole cells and organelles in terms of the spatial arrangements of their molecular components may soon become routine...
  11. pmc Electron tomography in nanoparticle imaging and analysis
    Jeffrey S Lengyel
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Nanomedicine (Lond) 3:125-31. 2008
    ....
  12. pmc Spiral architecture of the nucleoid in Bdellovibrio bacteriovorus
    Carmen Butan
    Laboratory of Cell Biology, National Cancer Institute, NIH, Building 50, Room 4306, 50 South Drive, Bethesda, MD 20892 8008, USA
    J Bacteriol 193:1341-50. 2011
    ..Our findings provide direct structural evidence for spiral organization of the bacterial nucleoid and suggest a possible role for MreB in regulation of nucleoid architecture and localization of the chemotaxis apparatus...
  13. pmc Prefusion structure of trimeric HIV-1 envelope glycoprotein determined by cryo-electron microscopy
    Alberto Bartesaghi
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    Nat Struct Mol Biol 20:1352-7. 2013
    ....
  14. pmc HIV-1 envelope glycoprotein trimers display open quaternary conformation when bound to the gp41 membrane-proximal external-region-directed broadly neutralizing antibody Z13e1
    Audray K Harris
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, USA
    J Virol 87:7191-6. 2013
    ..Our results support the idea that MPER-directed antibodies, such as Z13e1, block viral entry by interacting with Env at a step after CD4 activation...
  15. pmc Trimeric HIV-1 glycoprotein gp140 immunogens and native HIV-1 envelope glycoproteins display the same closed and open quaternary molecular architectures
    Audray Harris
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 108:11440-5. 2011
    ....
  16. pmc Three-dimensional imaging of the highly bent architecture of Bdellovibrio bacteriovorus by using cryo-electron tomography
    Mario J Borgnia
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, 50 South Drive, Bethesda, MD 20892, USA
    J Bacteriol 190:2588-96. 2008
    ..We propose that rearrangements of these structures, in combination with the unique properties of the cell envelope, may underlie the remarkable ability of B. bacteriovorus cells to find and enter bacterial prey...
  17. doi request reprint Cryo-electron tomography of bacteria: progress, challenges and future prospects
    Jacqueline L S Milne
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Rev Microbiol 7:666-75. 2009
    ..Possible future directions for whole cell imaging are also discussed...
  18. ncbi request reprint 3D imaging of mammalian cells with ion-abrasion scanning electron microscopy
    Jurgen A W Heymann
    Laboratory of Cell Biology, Center for Cancer Research National Cancer Institute, NIH, Building 50, Room 4306, Bethesda, MD 20892, USA
    J Struct Biol 166:1-7. 2009
    ..IA-SEM is thus a useful tool for imaging large mammalian cells in their entirety at resolutions in the nanometer range...
  19. pmc Evaluation of denoising algorithms for biological electron tomography
    Rajesh Narasimha
    Laboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    J Struct Biol 164:7-17. 2008
    ..We validate this strategy of automated segmentation of optimally denoised tomograms by comparing its performance with manual extraction of key features from the same tomograms...
  20. ncbi request reprint Stoichiometry and absolute quantification of proteins with mass spectrometry using fluorescent and isotope-labeled concatenated peptide standards
    Dhaval Nanavati
    Laboratory of Neurotoxicology, National Institute of Mental Health, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Proteomics 7:442-7. 2008
    ....
  21. pmc Molecular architectures of trimeric SIV and HIV-1 envelope glycoproteins on intact viruses: strain-dependent variation in quaternary structure
    Tommi A White
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, United States of America
    PLoS Pathog 6:e1001249. 2010
    ....