Research Topics
Genomes and Genes | B A MerrickSummaryAffiliation: National Institutes of Health Country: USA Publications
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Publications
Testing an aflatoxin B1 gene signature in rat archival tissuesB Alex Merrick
Biomolecular Screening Branch, Division of the National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, United States
Chem Res Toxicol 25:1132-44. 2012..We conclude that an evaluation of gene signatures in archival tissues can be an important toxicological tool for evaluating critical molecular events associated with chemical exposures...
NGF-mediated transcriptional targets of p53 in PC12 neuronal differentiationChristopher Brynczka
National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
BMC Genomics 8:139. 2007..We hypothesized that p53 contributed to PC12 differentiation through the regulation of gene targets distinct from its known transcriptional targets for apoptosis or DNA repair...
Complementary gene and protein expression studies and integrative approaches in toxicogenomicsB Alex Merrick
National Center for Toxicogenomics, National Institute of Environmental Health Sciences, D2 04, P O Box 12233, Research Triangle Park, NC 27709, USA
Toxicol Appl Pharmacol 207:189-94. 2005....- Induction of novel Grp75 isoforms by 2-deoxyglucose in human and murine fibroblastsB A Merrick
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle, NC 27709, USA
Cancer Lett 119:185-90. 1997..However, glucose deprivation by 2-deoxyglucose treatment induced five novel isoforms at 74-75 kDa mass. Mortalin at 66 kDa could not be detected under these treatment conditions... - HSP binding and mitochondrial localization of p53 protein in human HT1080 and mouse C3H10T1/2 cell linesB A Merrick
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
Biochim Biophys Acta 1297:57-68. 1996..These studies suggest that newly identified members of p53:HSP complexes represent components of a chaperone program which affects the subcellular distribution of p53 protein in these transformed lines... - The plasma proteome, adductome and idiosyncratic toxicity in toxicoproteomics researchB Alex Merrick
National Center for Toxicogenomics, National Institute of Environmental Health Sciences, PO Box 12233, Research Triangle Park, NC 27709, USA
Brief Funct Genomic Proteomic 7:35-49. 2008.... - Toxicoproteomics in liver injury and inflammationB Alex Merrick
National Institutes of Environmental Health Sciences, Research Triangle Park, Durham, NC 27709, USA
Ann N Y Acad Sci 1076:707-17. 2006..The new field of toxicoproteomics is uniquely positioned toward discovery of new biomarkers and signatures of tissue injury and a better understanding of protein expression responses during toxicity and environmental disease... - The role of toxicoproteomics in assessing organ specific toxicityB Alex Merrick
Laboratory of Respiratory Biology, National Institute of Environmental Health Sciences NIEHS, Research Triangle Park, Durham, NC 27709, USA
EXS 99:367-400. 2009..The final section suggests several key developments involving new approaches and research focus areas for the field of toxicoproteomics as a new tool for toxicological pathology... - Genomic and proteomic profiling for biomarkers and signature profiles of toxicityB Alex Merrick
National Center for Toxicogenomics, National Institute of Environmental Health Sciences, PO Box 12233, Research Triangle Park, NC 27709, USA
Curr Opin Mol Ther 6:600-7. 2004.... - Alterations in the rat serum proteome during liver injury from acetaminophen exposureB Alex Merrick
Proteomics Group, National Institute of Environmental Health Sciences, D2 04, P O Box 12233, Research Triangle Park, NC 27709, USA
J Pharmacol Exp Ther 318:792-802. 2006..This study demonstrates that serum proteome alterations probably reflect both liver damage and a concerted, complex response of the body for organ repair and recovery during acute hepatic injury... - The human proteome organization (HUPO) and environmental healthB Alex Merrick
Proteomics Group, National Center for Toxicogenomics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
EHP Toxicogenomics 111:1-5. 2003..Environmental health research stands to greatly benefit from the shared resources, data, and vision of the HUPO organization as a valuable resource in exploiting knowledge of the human proteome toward improving public health... - Genetic events associated with arsenic-induced malignant transformation: applications of cDNA microarray technologyH Chen
Laboratory of Comparative Carcinogenesis, NIEHS, Research Triangle Park, North Carolina 27709, USA
Mol Carcinog 30:79-87. 2001..Mol. Carcinog. 30:79-87, 2001. Published 2001 Wiley-Liss, Inc... - Comparison of 14C-amino acid mixture and [35S]methionine labeling of cellular proteins from mouse fibroblast C3H10T1/2 cells by two-dimensional gel electrophoresisC Y He
Division of Toxicology Research and Testing, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709
Electrophoresis 12:658-66. 1991..Thus, 35S-Met appears better suited for short-term radiolabeling of cellular protein while more comprehensive labeling of protein occurs with 14C-AA during prolonged incubation of cell cultures under present experimental conditions... - Tumor suppressor p53 gene forms multiple isoforms: evidence for single locus origin and cytoplasmic complex formation with heat shock proteinsJ K Selkirk
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA
Electrophoresis 17:1764-71. 1996..Cytoplasmic complexes are most likely formed to protect p53 from proteolysis and are probably involved in translocation of activated p53 from the cytoplasm to the nucleus for transactivation of other cell cycle control genes... - Separation and sequencing of familiar and novel murine proteins using preparative two-dimensional gel electrophoresisB A Merrick
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, NC
Electrophoresis 15:735-45. 1994..Thus, we have found that preparative 2-D PAGE serves as a powerful one-step purification method for protein isolation and characterization from an important in vitro murine model for the study of carcinogenesis... - Global gene expression associated with hepatocarcinogenesis in adult male mice induced by in utero arsenic exposureJie Liu
Inorganic Carcinogenesis Section, Laboratory of Comparative Carcinogenesis, National Cancer Institute, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
Environ Health Perspect 114:404-11. 2006..These novel findings are in agreement with the biology and histology of arsenic-induced HCC, thereby indicating that multiple genetic events are associated with transplacental arsenic hepatocarcinogenesis... - Multiple p53 protein isoforms and formation of oligomeric complexes with heat shock proteins Hsp70 and Hsp90 in the human mammary tumor, T47D, cell lineJ K Selkirk
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709
Appl Theor Electrophor 4:11-8. 1994..After translocation, p53 is freed from Hsp proteins for binding to DNA where Hsp70 and Hsp90 are no longer able to form a nuclear complex probably rendering Hsp's labile to proteolysis... - Two dimensional gel electrophoresis of cellular and secreted proteins from rat alveolar macrophages after lipopolysaccharide treatmentB A Merrick
Division of Toxicology Research and Testing, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709
Appl Theor Electrophor 2:177-87. 1992.... - An intragenic deletion of nuclear localization signal-1 of p53 tumor suppressor gene results in loss of apoptosis in murine fibroblastsW Zhao
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Science, Research Triangle Park, NC 27709, USA
Cancer Lett 147:101-8. 1999..Analysis of p53 protein from subcellular fractions indicated that NLS-I deprived p53 remained in the cytoplasmic fraction, which may explain why NLS-I deleted p53 failed to induce apoptosis... - Gel electrophoretic analysis of cellular and secreted proteins from resting and activated rat alveolar macrophages treated with pentamidine isethionateJ K Selkirk
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
J Chromatogr A 711:331-7. 1995..Thus pentamidine exhibited a general repressive effect on cellular and secreted protein expression in resting and activated macrophages... - Transplacental arsenic plus postnatal 12-O-teradecanoyl phorbol-13-acetate exposures associated with hepatocarcinogenesis induce similar aberrant gene expression patterns in male and female mouse liverJie Liu
Inorganic Carcinogenesis Section, Laboratory of Comparative Carcinogenesis, National Cancer Institute at NIEHS, Mail Drop F0 09, Research Triangle Park, NC 27709, USA
Toxicol Appl Pharmacol 213:216-23. 2006.... - Chemical effects in biological systems--data dictionary (CEBS-DD): a compendium of terms for the capture and integration of biological study design description, conventional phenotypes, and 'omics dataJennifer Fostel
LIMT Lockheed Martin Information Technology LMIT, Research Triangle Park, NC 27709, USA
Toxicol Sci 88:585-601. 2005..To illustrate the utility of the CEBS-DD, we present an example of integrating data from two proteomics and transcriptomics studies of the response to acute acetaminophen toxicity (A. N. Heinloth et al., 2004, Toxicol. Sci. 80, 193-202)... - Differential gene expression profiling in whole blood during acute systemic inflammation in lipopolysaccharide-treated ratsRick D Fannin
National Center for Toxicogenomics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
Physiol Genomics 21:92-104. 2005..These studies indicate that, in whole blood, changes in gene expression profiles can be detected that are reflective of inflammation, despite the adaptive shifts in leukocyte populations that accompany such inflammatory processes... - Two-dimensional polyacrylamide gel electrophoretic characterization of proteins from organs of C3H mice expressing the scurfy (sf) genetic mutation during early and late stages of disease progressionJ K Selkirk
Division of Toxicology Research and Testing, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709
Appl Theor Electrophor 3:97-107. 1992..These putative biomarkers may be useful for characterizing the sf mutation and the mutant may act a possible model the Wiskott-Aldrich syndrome (WAS)... - HSP70-2 is part of the synaptonemal complex in mouse and hamster spermatocytesJ W Allen
National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
Chromosoma 104:414-21. 1996..These findings are suggestive of a previously unsuspected sexual dimorphism in structure and/or function of the synaptonemal complex... - Quantitative proteomics analysis of macrophage rafts reveals compartmentalized activation of the proteasome and of proteasome-mediated ERK activation in response to lipopolysaccharideSuraj Dhungana
Laboratories of Respiratory Biology, NIEHS, National Institutes of Health, United States Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
Mol Cell Proteomics 8:201-13. 2009..Taken together, our findings indicate a critical, selective role for raft compartmentalization and regulation of proteasome activity in activation of the MEK-ERK pathway... - Site-specific phosphorylation of human p53 protein determined by mass spectrometryB A Merrick
Laboratory of Molecular Carcinogenesis and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
Biochemistry 40:4053-66. 2001..In summary, MS analysis uniquely revealed increased, site-specific phosphorylations on p53 after phosphatase inhibition, particularly at Ser(315), which may be critical molecular events in defining p53 activity... - CEBS--Chemical Effects in Biological Systems: a public data repository integrating study design and toxicity data with microarray and proteomics dataMichael Waters
NIEHS, National Center for Toxicogenomics, PO Box 12233, Research Triangle Park, NC 27709, USA
Nucleic Acids Res 36:D892-900. 2008..niehs.nih.gov. BID can be accessed via the user interface from https://dir-apps.niehs.nih.gov/arc/. Requests for a copy of BID and for depositing data into CEBS or BID are available at http://www.niehs.nih.gov/cebs-df/... - Association of c-myc overexpression and hyperproliferation with arsenite-induced malignant transformationH Chen
Laboratory of Comparative Carcinogenesis, National Cancer Institute (NCI) at National Institute of Environmental Health Sciences (NIEHS, Research Triangle Park, NC 27709, USA
Toxicol Appl Pharmacol 175:260-8. 2001..These correlations provide convincing evidence c-myc overexpression is mechanistically important in arsenic-induced malignant transformation in this model system... - Proliferating cell nuclear antigen staining of Fischer-344/N rat spleens affected by large granular lymphocyte leukemiaS A Stefanski
Laboratory of Molecular Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
Toxicol Pathol 23:1-6. 1995..These results support biological significance for the morphologic staging system currently in use... - Myeloid differentiation primary response protein 88 couples reverse cholesterol transport to inflammationKathleen A Smoak
Laboratory of Respiratory Biology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
Cell Metab 11:493-502. 2010.... - Toxicoproteomics: proteomics applied to toxicology and pathologyBarbara A Wetmore
National Center for Toxicogenomics, National Institute of Environmental Health Sciences, Research Triangle Park, North Caroline 27709, USA
Toxicol Pathol 32:619-42. 2004..The new field of toxicoproteomics is uniquely positioned toward an expanded understanding of protein expression during toxicity and environmental disease for the advancement of public health... - Apoptosis mediated by p53 in rat neural AF5 cells following treatment with hydrogen peroxide and staurosporineCharlesene McNeill-Blue
Proteomics Group, National Center for Toxicogenomics, National Institute of Environmental Health Sciences, National Institute of Health, Department of Health and Human Services, D2-04, P.O. Box 12233, Research Triangle Park, NC 27709, USA
Brain Res 1112:1-15. 2006.... - Effects of TCDD upon IkappaB and IKK subunits localized in microsomes by proteomicsMaribel E Bruno
Proteomics Group, National Center for Toxicogenomics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
Arch Biochem Biophys 406:153-64. 2002..Overall, proteomic analysis indicated the presence of NF-kappaB pathway members in microsomes, selectively altered by dioxin, which may influence immune and inflammatory responses within the liver... - Exploration of low-dose estrogen effects: identification of No Observed Transcriptional Effect Level (NOTEL)Edward K Lobenhofer
National Center for Toxicogenomics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
Toxicol Pathol 32:482-92. 2004..For example, in the development of therapeutic drugs, a NOTEL could be used to identify doses of pharmaceutical compounds that are no longer effective at modulating the expression of biomarkers of efficacy... - Gene expression response in target organ and whole blood varies as a function of target organ injury phenotypeEdward K Lobenhofer
Cogenics, Division of Clinical Data, Inc, Morrisville, NC 27560, USA
Genome Biol 9:R100. 2008..The results of the study demonstrate the classification of histopathological differences, likely reflecting differences in mechanisms of cell-specific toxicity, using either liver tissue or blood transcriptomic data... - Toxicoproteomics: a parallel approach to identifying biomarkersB Alex Merrick
Environ Health Perspect 111:A578-9. 2003 - Chemical effects in biological systems (CEBS) object model for toxicology data, SysTox-OM: design and applicationSandhya Xirasagar
Science Applications International Corporation, 20201 Century Boulevard, 3rd Floor, Germantown, MD 20874, USA
Bioinformatics 22:874-82. 2006..All of these data types can be integrated in a seamless fashion to enable data query and analysis in a biologically meaningful manner... - CEBS object model for systems biology data, SysBio-OMSandhya Xirasagar
Science Applications International Corporation, 20201 Century Building, 3rd Floor, Germantown, MD 20874, USA
Bioinformatics 20:2004-15. 2004..Data streams include, but are not limited to, gene expression analysis (transcriptomics), protein expression and protein-protein interaction analysis (proteomics) and changes in low molecular weight metabolite levels (metabolomics)...