Ronald P Mason

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Using anti-5,5-dimethyl-1-pyrroline N-oxide (anti-DMPO) to detect protein radicals in time and space with immuno-spin trapping
    Ronald P Mason
    The National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 36:1214-23. 2004
  2. pmc Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice
    Saurabh Chatterjee
    Free Radical Metabolites Group, Laboratory of Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Free Radic Biol Med 46:454-61. 2009
  3. ncbi request reprint Protein radical formation during lactoperoxidase-mediated oxidation of the suicide substrate glutathione: immunochemical detection of a lactoperoxidase radical-derived 5,5-dimethyl-1-pyrroline N-oxide nitrone adduct
    Qiong Guo
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, PO Box 12233, Research Triangle Park, NC 27709, USA
    J Biol Chem 279:13272-83. 2004
  4. pmc Site-specific radical formation in DNA induced by Cu(II)-H₂O₂ oxidizing system, using ESR, immuno-spin trapping, LC-MS, and MS/MS
    Suchandra Bhattacharjee
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 50:1536-45. 2011
  5. pmc Sulfite-mediated oxidation of myeloperoxidase to a free radical: immuno-spin trapping detection in human neutrophils
    Kalina Ranguelova
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 60:98-106. 2013
  6. ncbi request reprint Mechanism of hydrogen peroxide-induced Cu,Zn-superoxide dismutase-centered radical formation as explored by immuno-spin trapping: the role of copper- and carbonate radical anion-mediated oxidations
    Dario C Ramirez
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T W Alexander Drive, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 38:201-14. 2005
  7. pmc Protein Radical Formation Resulting from Eosinophil Peroxidase-catalyzed Oxidation of Sulfite
    Kalina Ranguelova
    Laboratory of Pharmacology, NIEHS, National Institutes of Health, Research Triangle Park, NC 27709, USA
    J Biol Chem 285:24195-205. 2010
  8. pmc Lipid-derived free radical production in superantigen-induced interstitial pneumonia
    Hisako Miyakawa
    Free Radical Metabolite Section, Laboratory of Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 47:241-9. 2009
  9. ncbi request reprint Immunological identification of the heart myoglobin radical formed by hydrogen peroxide
    Charles D Detweiler
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 33:364-9. 2002
  10. pmc Identifying the site of spin trapping in proteins by a combination of liquid chromatography, ELISA, and off-line tandem mass spectrometry
    Olivier M Lardinois
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 44:893-906. 2008

Detail Information

Publications96

  1. ncbi request reprint Using anti-5,5-dimethyl-1-pyrroline N-oxide (anti-DMPO) to detect protein radicals in time and space with immuno-spin trapping
    Ronald P Mason
    The National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 36:1214-23. 2004
    ..In time, all of the immunological techniques based on antibody-nitrone binding should become available for free radical detection in a wide variety of biological systems...
  2. pmc Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice
    Saurabh Chatterjee
    Free Radical Metabolites Group, Laboratory of Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Free Radic Biol Med 46:454-61. 2009
    ..This suggests the presence of a possible mechanism for CPB1 activity loss with compensatory protein production...
  3. ncbi request reprint Protein radical formation during lactoperoxidase-mediated oxidation of the suicide substrate glutathione: immunochemical detection of a lactoperoxidase radical-derived 5,5-dimethyl-1-pyrroline N-oxide nitrone adduct
    Qiong Guo
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, PO Box 12233, Research Triangle Park, NC 27709, USA
    J Biol Chem 279:13272-83. 2004
    ....
  4. pmc Site-specific radical formation in DNA induced by Cu(II)-H₂O₂ oxidizing system, using ESR, immuno-spin trapping, LC-MS, and MS/MS
    Suchandra Bhattacharjee
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 50:1536-45. 2011
    ..The DMPO adduct formed is initially EPR active but subsequently is oxidized to the stable nitrone, which can then be detected by IST and further characterized by MS and MS/MS...
  5. pmc Sulfite-mediated oxidation of myeloperoxidase to a free radical: immuno-spin trapping detection in human neutrophils
    Kalina Ranguelova
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 60:98-106. 2013
    ..Our present study demonstrates that myeloperoxidase initiates (bi)sulfite oxidation leading to MPO radical damage, possibly leading to (bi)sulfite-exacerbated allergic reactions...
  6. ncbi request reprint Mechanism of hydrogen peroxide-induced Cu,Zn-superoxide dismutase-centered radical formation as explored by immuno-spin trapping: the role of copper- and carbonate radical anion-mediated oxidations
    Dario C Ramirez
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T W Alexander Drive, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 38:201-14. 2005
    ..These results help clarify the free radical chemistry involved in the functional and structural oxidative damage to SOD1 by H2O2 with the intermediacy of copper- and CO3*--mediated oxidations...
  7. pmc Protein Radical Formation Resulting from Eosinophil Peroxidase-catalyzed Oxidation of Sulfite
    Kalina Ranguelova
    Laboratory of Pharmacology, NIEHS, National Institutes of Health, Research Triangle Park, NC 27709, USA
    J Biol Chem 285:24195-205. 2010
    ..These studies suggest that EPO-dependent oxidative damage may play a role in tissue injury in bisulfite-exacerbated eosinophilic inflammatory disorders...
  8. pmc Lipid-derived free radical production in superantigen-induced interstitial pneumonia
    Hisako Miyakawa
    Free Radical Metabolite Section, Laboratory of Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 47:241-9. 2009
    ....
  9. ncbi request reprint Immunological identification of the heart myoglobin radical formed by hydrogen peroxide
    Charles D Detweiler
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 33:364-9. 2002
    ..Western blot analysis demonstrates that myoglobin forms the predominant radical-derived nitrone adduct in rat heart supernatant...
  10. pmc Identifying the site of spin trapping in proteins by a combination of liquid chromatography, ELISA, and off-line tandem mass spectrometry
    Olivier M Lardinois
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 44:893-906. 2008
    ..The results demonstrate the power of the new experimental strategy to select DMPO-labeled peptides and identify sites of DMPO covalent attachments...
  11. ncbi request reprint Immuno-spin trapping of DNA radicals
    Dario C Ramirez
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T W Alexander Dr, Research Triangle Park, North Carolina 27709, USA
    Nat Methods 3:123-7. 2006
    ..We also applied IST to detect DNA radicals in rat hepatocytes exposed to Cu(II) and H(2)O(2) under nonlethal conditions...
  12. pmc Evaluation of the Forrester-Hepburn mechanism as an artifact source in ESR spin-trapping
    Fabian Leinisch
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T W Alexander Drive, Research Triangle Park, North Carolina 27709, USA
    Chem Res Toxicol 24:2217-26. 2011
    ....
  13. pmc Aminoglutethimide-induced protein free radical formation on myeloperoxidase: a potential mechanism of agranulocytosis
    Arno G Siraki
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, 111 Alexander Drive, Research Triangle Park, North Carolina 27709, USA
    Chem Res Toxicol 20:1038-45. 2007
    ..We propose that drug-dependent free radical formation on MPO may play a role in the origin of agranulocytosis...
  14. pmc Procainamide, but not N-acetylprocainamide, induces protein free radical formation on myeloperoxidase: a potential mechanism of agranulocytosis
    Arno G Siraki
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, 111 Alexander Dr, Research Triangle Park, NC 27709, USA
    Chem Res Toxicol 21:1143-53. 2008
    ..We propose that drug-induced free radical formation on MPO may play a role in the origin of agranulocytosis...
  15. pmc Direct magnetic resonance evidence for peroxymonocarbonate involvement in the cu,zn-superoxide dismutase peroxidase catalytic cycle
    Marcelo G Bonini
    Laboratory of Pharmacology, NIEHS, National Institutes of Health, Research Triangle Park, NC, USA
    J Biol Chem 284:14618-27. 2009
    ..The results gathered herein indicate that peroxymonocarbonate (HOOCO(2)(-)) is a key intermediate in the SOD1 peroxidase cycle and identify this species as the precursor of carbonate radical anions...
  16. pmc Spin scavenging analysis of myoglobin protein-centered radicals using stable nitroxide radicals: characterization of oxoammonium cation-induced modifications
    Olivier M Lardinois
    Laboratories of Pharmacology and Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Chem Res Toxicol 22:1034-49. 2009
    ..g., by use of 2,4-dinitrophenyl hydrazine). The results reveal the pitfalls inherent in using stable nitroxide radicals such as TEMPOL to identify sites of radical formation on hemoproteins...
  17. pmc Cadmium generates reactive oxygen- and carbon-centered radical species in rats: insights from in vivo spin-trapping studies
    Jie Liu
    Inorganic Carcinogenesis Section, Laboratory of Comparative Carcinogenesis, National Cancer Institute at National Institute of Environmental Health Sciences National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 45:475-81. 2008
    ..This is the first study to show Cd generation of reactive oxygen- and carbon-centered radical species by involvement of both iron mediation through iron-catalyzed reactions and activation of Kupffer cells, the resident liver macrophages...
  18. pmc Direct evidence of iNOS-mediated in vivo free radical production and protein oxidation in acetone-induced ketosis
    Krisztian Stadler
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Am J Physiol Endocrinol Metab 295:E456-62. 2008
    ..Taken together, our data demonstrate that acetone administration, a model of ketosis, can lead to protein oxidation and lipid peroxidation through a free radical-dependent mechanism driven mainly by iNOS overexpression...
  19. pmc Identification of protein radicals formed in the human neuroglobin-H2O2 reaction using immuno-spin trapping and mass spectrometry
    Olivier M Lardinois
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Biochemistry 47:10440-8. 2008
    ..Together, the data support the argument that wild-type Ngb is protected from attack by H 2O 2 by the coordinated distal His...
  20. ncbi request reprint Electron transfer between a tyrosyl radical and a cysteine residue in hemoproteins: spin trapping analysis
    Suchandra Bhattacharjee
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    J Am Chem Soc 129:13493-501. 2007
    ..This complex phenomenon of electron transfer from one radical to another has been investigated in proteins by IST, ESR, and MS...
  21. pmc Site-specific carboxypeptidase B1 tyrosine nitration and pathophysiological implications following its physical association with nitric oxide synthase-3 in experimental sepsis
    Saurabh Chatterjee
    Free Radical Metabolism Group, Laboratory of Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    J Immunol 183:4055-66. 2009
    ..These results open up new avenues for the investigation of several enzymes involved in inflammation and their site-specific oxidative modifications by protein-protein interactions as well as their role in sepsis...
  22. pmc (Bi)sulfite oxidation by copper, zinc-superoxide dismutase: Sulfite-derived, radical-initiated protein radical formation
    Kalina Ranguelova
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    Environ Health Perspect 118:970-5. 2010
    ..This free radical further reacts with oxygen to form the peroxymonosulfate anion radical (-O(3)SOO*) and sulfate anion radical (SO(4)*-)...
  23. ncbi request reprint Synergistic production of lung free radicals by diesel exhaust particles and endotoxin
    Toyoko Arimoto
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Am J Respir Crit Care Med 171:379-87. 2005
    ..This is the first demonstration that DEP and LPS work synergistically to enhance free radical generation in lungs, mediated by the activation of local XO...
  24. pmc Immunological detection of N-formylkynurenine in oxidized proteins
    Marilyn Ehrenshaft
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 46:1260-6. 2009
    ..Results from mass spectrometric analysis of photooxidized myoglobin samples corroborate the immunological data, detecting an increase in NFK content as the extent of photooxidation increases...
  25. pmc Detection and imaging of the free radical DNA in cells--site-specific radical formation induced by Fenton chemistry and its repair in cellular DNA as seen by electron spin resonance, immuno-spin trapping and confocal microscopy
    Suchandra Bhattacharjee
    Laboratory of Toxicology and Chemistry, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, USA
    Nucleic Acids Res 40:5477-86. 2012
    ..In vivo experiments for the purpose of detecting DMPO-DNA nitrone adducts should be conducted over a range of time in order to avoid missing adducts due to the repair processes...
  26. pmc Oxidative stress induces protein and DNA radical formation in follicular dendritic cells of the germinal center and modulates its cell death patterns in late sepsis
    Saurabh Chatterjee
    National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 50:988-99. 2011
    ..In one such mechanism, radical formation initiated by xanthine oxidase formed protein and DNA radicals, which may lead to cell death of germinal center FDCs...
  27. ncbi request reprint Identification of the myoglobin tyrosyl radical by immuno-spin trapping and its dimerization
    Charles D Detweiler
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, PO Box 12233, MD F0 01, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 38:969-76. 2005
    ..Interestingly, formation of the myoglobin dimer, which is known to be formed primarily by cross-linkage of tyrosine-151, was inhibited by the addition of DMPO...
  28. ncbi request reprint Characterization of the high-resolution ESR spectra of superoxide radical adducts of 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxide (DEPMPO) and 5,5-dimethyl-1-pyrroline N-oxide (DMPO). Analysis of conformational exchange
    Sergey Dikalov
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Res 39:825-36. 2005
    ..It is clear that to correctly interpret DEPMPO spin trapping of superoxide radicals, one must carefully consider formation of secondary radical adducts...
  29. ncbi request reprint A novel protocol to identify and quantify all spin trapped free radicals from in vitro/in vivo interaction of HO(.-) and DMSO: LC/ESR, LC/MS, and dual spin trapping combinations
    Steven Yue Qian
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 38:125-35. 2005
    ..This novel protocol provides a comprehensive way to characterize and quantify in vitro and in vivo free radical formation and will have many applications in biological research...
  30. ncbi request reprint Identification of spin trapped carbon-centered radicals in soybean lipoxygenase-dependent peroxidations of omega-3 polyunsaturated fatty acids by LC/ESR, LC/MS, and tandem MS
    Steven Yue Qian
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 35:33-44. 2003
    ....
  31. pmc Ceruloplasmin (ferroxidase) oxidizes hydroxylamine probes: deceptive implications for free radical detection
    Douglas Ganini
    Free Radical Metabolism Section, Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 53:1514-21. 2012
    ..In conclusion, we show that the ferroxidase activity of ceruloplasmin is a possible biological source of artifacts in the cyclic hydroxylamine-oxidation assay used for reactive oxygen species detection and quantification...
  32. pmc Kinetics of the oxidation of reduced Cu,Zn-superoxide dismutase by peroxymonocarbonate
    Kalina Ranguelova
    Laboratory of Pharmacology and Toxicology, NIEHS, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 53:589-94. 2012
    ..It is proposed that the dissociation of His61 from the active site Cu(I) in SOD-Cu(I) contributes to this chemistry by facilitating the binding of larger anions, such as peroxymonocarbonate...
  33. ncbi request reprint Immunochemical detection of nitric oxide and nitrogen dioxide trapping of the tyrosyl radical and the resulting nitrotyrosine in sperm whale myoglobin
    Kozo Nakai
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, MD F0 01, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 39:1050-8. 2005
    ..The amount of nitrotyrosine generated with *NO was higher than with NO2-, implying that there are two different peroxynitrite-independent nitrotyrosine formation mechanisms and that *NO is not just a source of *NO2...
  34. pmc Requirement of arsenic biomethylation for oxidative DNA damage
    Chikara Kojima
    Inorganic Carcinogenesis Section, Laboratory of Comparative Carcinogenesis, Center for Cancer Research, National Cancer Institute at National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    J Natl Cancer Inst 101:1670-81. 2009
    ..We assessed the role of arsenic biomethylation in oxidative DNA damage (ODD) using a recently developed immuno-spin trapping method...
  35. pmc New insights into the detection of sulfur trioxide anion radical by spin trapping: radical trapping versus nucleophilic addition
    Kalina Ranguelova
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 47:128-34. 2009
    ..1 M and the incubations are for longer times...
  36. ncbi request reprint UVA-ketoprofen-induced hemoglobin radicals detected by immuno-spin trapping
    Yu Ying He
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Photochem Photobiol 77:585-91. 2003
    ..The successful detection of protein radical formation caused by KP photosensitization could help further understand the photoallergic effect of this NSAID...
  37. pmc Spin trapping investigation of peroxide- and isoniazid-induced radicals in Mycobacterium tuberculosis catalase-peroxidase
    Kalina Ranguelova
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, MD F0 01, 111 T W Alexander Drive, Research Triangle Park, North Carolina 27709, USA
    Biochemistry 47:11377-85. 2008
    ..Furthermore, the formation of protein-DMPO nitrone adducts was accelerated in the presence of isoniazid...
  38. ncbi request reprint Immunochemical detection of hemoglobin-derived radicals formed by reaction with hydrogen peroxide: involvement of a protein-tyrosyl radical
    Dario C Ramirez
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27713, USA
    Free Radic Biol Med 34:830-9. 2003
    ....
  39. pmc Targeted oxidation of Torpedo californica acetylcholinesterase by singlet oxygen: identification of N-formylkynurenine tryptophan derivatives within the active-site gorge of its complex with the photosensitizer methylene blue
    Mathilde M Triquigneaux
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, DHHS, PO Box 12233 MD F0 03, Research Triangle Park, NC 27709, USA
    Biochem J 448:83-91. 2012
    ....
  40. ncbi request reprint Novel identification of a sulfur-centered, radical-derived 5,5-dimethyl-1-pyrroline N-oxide nitrone adduct formed from the oxidation of DTT by LC/ELISA, LC/electrospray ionization-MS, and LC/tandem MS
    Qiong Guo
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, Research Triangle Park, North Carolina 27709, USA
    Chem Res Toxicol 17:1481-90. 2004
    ..The successful application of LC/ELISA, LC/MS, and LC/MS/MS in this study makes it possible to separate and identify the stable DMPO nitrone adducts derived from free radical metabolites generated in biological systems...
  41. pmc Top-down and bottom-up mass spectrometric characterization of human myoglobin-centered free radicals induced by oxidative damage
    Leesa J Deterding
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, MD F0 03, Research Triangle Park, North Carolina 27709, USA
    Anal Chem 79:6236-48. 2007
    ..Nonetheless, the bottom-up and top-down approaches to protein characterization are complementary rather than competitive approaches with each having its own utility...
  42. pmc Involvement of inducible nitric oxide synthase in hydroxyl radical-mediated lipid peroxidation in streptozotocin-induced diabetes
    Krisztian Stadler
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233 MD F0 02, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 45:866-74. 2008
    ..Taken together, our studies support inducible nitric oxide synthase as a significant source of EPR-detectable reactive intermediates, which leads to lipid peroxidation and may contribute to disease progression as well...
  43. ncbi request reprint EPR spectroscopy studies on the structural transition of nitrosyl hemoglobin in the arterial-venous cycle of DEANO-treated rats as it relates to the proposed nitrosyl hemoglobin/nitrosothiol hemoglobin exchange
    Adrian R Jaszewski
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA
    Free Radic Biol Med 35:444-51. 2003
    ..20% arterial). These results are not consistent with the recently proposed exchange of nitrosyl heme with the beta-93 nitrosothiol group of hemoglobin during the arterial-venous cycle...
  44. doi request reprint Photooxidation of Amplex Red to resorufin: implications of exposing the Amplex Red assay to light
    Fiona A Summers
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, North Carolina, USA
    Methods Enzymol 526:1-17. 2013
    ..In general, precautions should be taken to minimize exposure to light, including that from instrumental light, during measurement of oxidative stress with Amplex Red. ..
  45. pmc Hypericin-mediated photooxidative damage of α-crystallin in human lens epithelial cells
    Marilyn Ehrenshaft
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 60:347-54. 2013
    ..John's wort cannot adequately shield their lenses from hypericin-mediated photosensitized damage...
  46. pmc Formation of reactive sulfite-derived free radicals by the activation of human neutrophils: an ESR study
    Kalina Ranguelova
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 52:1264-71. 2012
    ..Therefore, we propose that the potential toxicity of (bi)sulfite during pulmonary inflammation or lung-associated diseases such as asthma may be related to free radical formation...
  47. pmc Glutathione-induced radical formation on lactoperoxidase does not correlate with the enzyme's peroxidase activity
    Marcelo G Bonini
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, MD, USA
    Free Radic Biol Med 42:985-92. 2007
    ....
  48. ncbi request reprint Protein radical formation on thyroid peroxidase during turnover as detected by immuno-spin trapping
    Marilyn Ehrenshaft
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 41:422-30. 2006
    ..Evidence that correlates iodine deficiencies with development of thyroid autoimmune disorders supports this conjecture...
  49. pmc The fidelity of spin trapping with DMPO in biological systems
    Kalina Ranguelova
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, 111 TW Alexander Drive, Research Triangle Park, NC 27709, USA
    Magn Reson Chem 49:152-8. 2011
    ....
  50. pmc Free radical production requires both inducible nitric oxide synthase and xanthine oxidase in LPS-treated skin
    Kozo Nakai
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, MD F0 01, Research Triangle Park, NC 27709, USA
    Proc Natl Acad Sci U S A 103:4616-21. 2006
    ..Our results imply that both iNOS and XO, but neither NADPH oxidase nor ferric iron, work synergistically to form lipid radical and nitrotyrosine early in the skin inflammation caused by LPS...
  51. ncbi request reprint Copper-catalyzed protein oxidation and its modulation by carbon dioxide: enhancement of protein radicals in cells
    Dario C Ramirez
    Laboratory of Pharmacology and Chemistry, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 280:27402-11. 2005
    ..The pathophysiological significance of our findings is discussed...
  52. ncbi request reprint Noninvasive diagnostic tool for inflammation-induced oxidative stress using electron spin resonance spectroscopy and an extracellular cyclic hydroxylamine
    Sergey I Dikalov
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    Arch Biochem Biophys 402:218-26. 2002
    ..Diphenyleneiodonium (0.1 mM) inhibited formation of LPS-stimulated reactive oxidant species by 80%. We suggest that this test could be used as a noninvasive diagnostic tool for inflammation-induced oxidative stress...
  53. ncbi request reprint Identification of all classes of spin-trapped carbon-centered radicals in soybean lipoxygenase-dependent lipid peroxidations of omega-6 polyunsaturated fatty acids via LC/ESR, LC/MS, and tandem MS
    Steven Yue Qian
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA qian
    Free Radic Biol Med 34:1017-28. 2003
    ..The major beta-scission products in peroxidations of omega-6 PUFAs were the pentyl radicals. The ratio of beta-scission products, however, varied significantly depending on pH, [PUFA], as well as [O(2)]...
  54. ncbi request reprint Protein oxidation of cytochrome C by reactive halogen species enhances its peroxidase activity
    Yeong Renn Chen
    Laboratory of Pharmacology and Chemistry and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 277:29781-91. 2002
    ....
  55. pmc P2X7 receptor-NADPH oxidase axis mediates protein radical formation and Kupffer cell activation in carbon tetrachloride-mediated steatohepatitis in obese mice
    Saurabh Chatterjee
    Free Radical Metabolism Group, Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 52:1666-79. 2012
    ..We conclude that the P2X7 receptor is a primary mediator of oxidative stress-induced exacerbation of inflammatory liver injury in obese mice via NADPH oxidase-dependent mechanisms...
  56. pmc Studies on the photosensitized reduction of resorufin and implications for the detection of oxidative stress with Amplex Red
    Baozhong Zhao
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 51:153-9. 2011
    ..The suppression of oxygen consumption by addition of SOD or catalase further confirmed the production of superoxide and hydrogen peroxide...
  57. ncbi request reprint Characterization of the high resolution ESR spectra of the methoxyl radical adducts of 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxide (DEPMPO)
    Sergey Dikalov
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences National Institutes of Health, 111 Alexander Drive, PO Box 12233, Research Triangle Park, NC 27709, USA
    Free Radic Res 37:705-12. 2003
    ..We suggest that the analysis of high resolution ESR spectra can be used for the unambiguous characterization of DEPMPO radical adducts...
  58. ncbi request reprint Identification of free radicals on hemoglobin from its self-peroxidation using mass spectrometry and immuno-spin trapping: observation of a histidinyl radical
    Leesa J Deterding
    Laboratory of Structural Biology, Laboratory of Pharmacology and Chemistry, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 279:11600-7. 2004
    ..Because of the pi-pi interaction of Tyr-24 and His-20, the unpaired electron is apparently delocalized on both the tyrosine and histidine residue (pi-pi stacked pair radical)...
  59. pmc Immuno-spin trapping analyses of DNA radicals
    Dario C Ramirez
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, 111 TW Alexander Drive, Building 101, MD F0 02, Research Triangle Park, North Carolina 27709, USA
    Nat Protoc 2:512-22. 2007
    ..Because both protein and DNA nitrone adducts are formed, it is important that the DNA be pure in order to avoid misinterpretations. Depending on the model under study, this protocol can be completed in as few as 6 h...
  60. doi request reprint Catalase has a key role in protecting cells from the genotoxic effects of monomethylarsonous acid: a highly active metabolite of arsenic
    Jorge G Muñiz Ortiz
    Integrated Systems Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Environ Mol Mutagen 54:317-26. 2013
    ..These experiments suggest that CAT is involved in protecting cells against the genotoxic effects of the ·OH generated by MMA(III) ...
  61. pmc An electron paramagnetic resonance investigation of the oxygen dependence of the arterial-venous gradient of nitrosyl hemoglobin in blood circulation
    Jinjie Jiang
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 43:1208-15. 2007
    ..These results do not support the existence of an arterial-venous gradient of HbNO under our experimental conditions...
  62. ncbi request reprint Separation and identification of DMPO adducts of oxygen-centered radicals formed from organic hydroperoxides by HPLC-ESR, ESI-MS and MS/MS
    Qiong Guo
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Am Soc Mass Spectrom 14:862-71. 2003
    ..60 G and a(H)(beta) = 15.61 G in the reaction mixture containing cumene hydroperoxide), previously assigned as DMPO adducts of t-butyloxyl and cumyloxyl radical, were indeed from trapping t-butyloxyl and cumyloxyl radicals, respectively...
  63. doi request reprint NOS-1-derived NO is an essential triggering signal for the development of systemic inflammatory responses
    Danielle Duma
    Laboratory of Signal Transduction, Institute of Environmental Health Sciences, National Institute of Environmental Health Sciences NIH, Research Triangle Park, NC, USA
    Eur J Pharmacol 668:285-92. 2011
    ....
  64. pmc Partial colocalization of oxidized, N-formylkynurenine-containing proteins in mitochondria and Golgi of keratinocytes
    Marilyn Ehrenshaft
    Laboratory of Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA
    Photochem Photobiol 86:752-6. 2010
    ..These experiments demonstrate that we have developed a tool for the specific detection of oxidized tryptophan residues in cells and suggest that this tool could be useful in tracking the fate of these oxidized proteins...
  65. pmc Immunolocalization of hypochlorite-induced, catalase-bound free radical formation in mouse hepatocytes
    Marcelo G Bonini
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, 111 T W Alexander Drive, MD F0 02, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 42:530-40. 2007
    ....
  66. pmc L-tryptophan radical cation electron spin resonance studies: connecting solution-derived hyperfine coupling constants with protein spectral interpretations
    Henry D Connor
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, Research Triangle Park, North Carolina 27709, USA
    J Am Chem Soc 130:6381-7. 2008
    ..This approach also produces geometric parameters (dihedral angles for the beta-methylene hydrogens) that should facilitate protein site assignment of observed l-tryptophan radicals as has been done for l-tyrosine radicals...
  67. ncbi request reprint In vivo lipid-derived free radical formation by NADPH oxidase in acute lung injury induced by lipopolysaccharide: a model for ARDS
    Keizo Sato
    Free Radical Metabolite Section, Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    FASEB J 16:1713-20. 2002
    ..Rats treated intratracheally with LPS generate lipid-derived free radicals via activation of NADPH oxidase...
  68. ncbi request reprint The oxidation of 2',7'-dichlorofluorescin to reactive oxygen species: a self-fulfilling prophesy?
    Marcelo G Bonini
    Laboratory of Pharmacology and Chemistry, Ntional Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 40:968-75. 2006
    ..Our findings demonstrate that this assay is subject to a serious artifact in that it produces what it is purported to measure; therefore, its use in biological systems should be approached with caution...
  69. ncbi request reprint Characterization of the initial carbon-centered pentadienyl radical and subsequent radicals in lipid peroxidation: identification via on-line high performance liquid chromatography/electron spin resonance and mass spectrometry
    Steven Yue Qian
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 33:998-1009. 2002
    ..were detected and identified. The optimization of chromatography in the LC/ESR and MS combination provided a reliable and sensitive way for the detection and identification of expected radical adducts in vitro and in vivo...
  70. ncbi request reprint In vivo copper-mediated free radical production: an ESR spin-trapping study
    Maria B Kadiiska
    National Institutes of Health, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709 2233, USA
    Spectrochim Acta A Mol Biomol Spectrosc 58:1227-39. 2002
    ..If these precautions were not taken, radical adducts generated ex vivo could be mistaken for radical adducts produced in vivo and excreted into the bile...
  71. ncbi request reprint Nitric oxide trapping of the tyrosyl radical-chemistry and biochemistry
    Michael R Gunther
    Laboratory of Pharmacology and Chemistry, NIEHS NIH, Research Triangle Park, NC 27709, USA
    Toxicology 177:1-9. 2002
    ..The results provide a mechanism for nitric oxide-dependent tyrosine nitration that does not require formation of more highly reactive nitrogen oxide intermediates such as peroxynitrite or nitrogen dioxide...
  72. ncbi request reprint Immobilized enzyme electron spin resonance: a method for detecting enzymatically generated transient radicals
    Bradley E Sturgeon
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Anal Chem 75:5006-11. 2003
    ..An extension of this technique to ESR spin trapping has also been discussed...
  73. pmc Constitutive nitric oxide synthase activation is a significant route for nitroglycerin-mediated vasodilation
    Marcelo G Bonini
    Laboratories of Pharmacology and Chemistry and Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Proc Natl Acad Sci U S A 105:8569-74. 2008
    ..Our studies in cell cultures, isolated vessels, and whole animals identified endothelial NOS activation as a fundamental requirement for nitroglycerin action at pharmacologically relevant concentrations in WT animals...
  74. ncbi request reprint Acute cadmium exposure induces stress-related gene expression in wild-type and metallothionein-I/II-null mice
    Jie Liu
    Laboratory of Comparative Carcinogenesis, Mail Drop F0 09, NCI at NIEHS, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 32:525-35. 2002
    ..Thus, the mechanism of acute cadmium toxicity involves multiple facets including oxidative damage and aberrant gene expression, and absence of MT exacerbates Cd-induced aberrant gene expression...
  75. pmc Biomarkers of Oxidative Stress Study IV: ozone exposure of rats and its effect on antioxidants in plasma and bronchoalveolar lavage fluid
    Maria B Kadiiska
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 51:1636-42. 2011
    ..We conclude that measurements of antioxidants in plasma are not sensitive biomarkers for oxidative damage induced by ozone and are not a useful choice for the assessment of oxidative damage by ozone in vivo...
  76. pmc Simplified synthesis of isotopically labeled 5,5-dimethyl-pyrroline N-oxide
    Fabian Leinisch
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, 111 TW Alexander Drive, Research Triangle Park, NC 27709, USA
    Molecules 16:8428-36. 2011
    ..This facile, straightforward process allows synthesizing isotopically labeled DMPO analogs in a 4-step reaction without special equipment...
  77. pmc Identification of protein-derived tyrosyl radical in the reaction of cytochrome c and hydrogen peroxide: characterization by ESR spin-trapping, HPLC and MS
    Steven Yue Qian
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, Research Triangle Park, NC 27709, U S A
    Biochem J 363:281-8. 2002
    ..We conclude that the cytochrome c-derived tyrosyl radical was trapped by MNP, leading to a persistent radical adduct at the C-3/C-5 positions of the tyrosine phenyl ring...
  78. ncbi request reprint Electron spin resonance investigation of semiquinone radicals formed from the reaction of ubiquinone 0 with human oxyhemoglobin
    Qiong Guo
    Laboratories of Pharmacology and Chemistry, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 277:6104-10. 2002
    ..Subsequently UQ(0) bound to the beta-93 cysteinyl residue of HbO(2) to form a covalent-binding adduct responsible for the broad spectrum...
  79. pmc Mechanism in the reaction of cytochrome c oxidase with organic hydroperoxides: an ESR spin-trapping investigation
    Yeong Renn Chen
    The Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Biochem J 365:461-9. 2002
    ....
  80. ncbi request reprint DNA damage induced by methylated trivalent arsenicals is mediated by reactive oxygen species
    Stephen Nesnow
    Environmental Carcinogenesis Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Chem Res Toxicol 15:1627-34. 2002
    ..These data are consistent with the conclusions that the DNA-damaging activity of DMA(III) is an indirect genotoxic effect mediated by ROS-formed concomitantly with the oxidation of DMA(III) to DMA(V)...
  81. ncbi request reprint Fast-flow EPR spectroscopic observation of the isoniazid, iproniazid, and phenylhydrazine hydrazyl radicals
    Herbert J Sipe
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, P O Box 12233, Research Triangle Park, North Carolina 27709, USA
    Chem Res Toxicol 17:226-33. 2004
    ..Theoretical values of hyperfine coupling constants, calculated by density functional formalism, provided a guide to assignments for the hydrazyl species and confirmed the experimentally based assignments for phenylhydrazyl radical...
  82. ncbi request reprint The CYP inhibitor 1-aminobenzotriazole does not prevent oxidative stress associated with alcohol-induced liver injury in rats and mice
    Fuyumi Isayama
    Laboratory of Hepatobiology and Toxicology, Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Free Radic Biol Med 35:1568-81. 2003
    ..These data support the hypothesis that oxidative stress and liver damage can occur independently of CYP activities in both rats and mice during early alcohol-induced liver injury...
  83. ncbi request reprint Immuno-spin trapping of hemoglobin and myoglobin radicals derived from nitrite-mediated oxidation
    Agnes Keszler
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 40:507-15. 2006
    ..The immuno-spin trapping technique represents an important new development for the study of the range and extent of protein oxidation by free radicals and oxidants...
  84. ncbi request reprint Involvement of protein radical, protein aggregation, and effects on NO metabolism in the hypochlorite-mediated oxidation of mitochondrial cytochrome c
    Yeong Renn Chen
    607 Davis Heart and Lung Research Institute, Division of Cardiovascular Medicine, Department of Internal Medicine, Ohio State University, College of Medicine, 473 West, 12th Avenue Columbus, OH 43210, USA
    Free Radic Biol Med 37:1591-603. 2004
    ..Furthermore, HOCl-mediated cyt c oxidation also resulted in a significant elevation of cyt c nitration derived from either NO trapping of the cyt c-derived tyrosyl radical or cyt c-catalyzed one-electron oxidation of nitrite...
  85. ncbi request reprint Revisiting the interaction of the radical anion metabolite of nitrofurantoin with glutathione
    Catherine Miller
    Department of Chemistry, John Carroll University, Cleveland, Ohio 44118, USA
    Arch Biochem Biophys 397:113-8. 2002
    ....
  86. ncbi request reprint Cupric-amyloid beta peptide complex stimulates oxidation of ascorbate and generation of hydroxyl radical
    Sergey I Dikalov
    FRIMCORE, Division of Cardiology, Emory Medical School, Atlanta, GA 30322, USA
    Free Radic Biol Med 36:340-7. 2004
    ..Therefore, cupric-amyloid beta peptide-stimulated free radical generation may be involved in the pathogenesis of Alzheimer's disease...
  87. ncbi request reprint Peroxyl adduct radicals formed in the iron/oxygen reconstitution reaction of mutant ribonucleotide reductase R2 proteins from Escherichia coli
    Margareta Sahlin
    Department of Molecular Biology and Functional Genomics, Stockholm University, 106 91 Stockholm, Sweden
    J Biol Inorg Chem 7:74-82. 2002
    ..Structural arguments and experimental results favor W48 as the major site of peroxyl adducts in the mutant Y122F. Available molecular oxygen can be considered as a spin trap for surface-located protein free radicals...
  88. pmc Sustained formation of alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone radical adducts in mouse liver by peroxisome proliferators is dependent upon peroxisome proliferator-activated receptor-alpha, but not NADPH oxidase
    Courtney G Woods
    Department of Environmental Sciences and Engineering, University of North Carolina, 0031 Michael Hooker Research Center, Chapel Hill, NC 27599 7431, USA
    Free Radic Biol Med 42:335-42. 2007
    ..Therefore, peroxisome proliferator-induced POBN radical production in Kupffer cells may be limited to an acute response to these compounds in mouse liver...
  89. ncbi request reprint Formation of protein tyrosine ortho-semiquinone radical and nitrotyrosine from cytochrome c-derived tyrosyl radical
    Yeong Renn Chen
    Davis Heart and Lung Research Institute, Division of Cardiovascular Medicine, Department of Internal Medicine, College of Medicine, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 279:18054-62. 2004
    ..The results of this study may provide useful information in understanding the interconnection among cyt c, H(2)O(2), NO, and apoptosis...
  90. ncbi request reprint The Mechanism by which 4-hydroxy-2,2,6,6-tetramethylpiperidene-1-oxyl (tempol) diverts peroxynitrite decomposition from nitrating to nitrosating species
    Marcelo G Bonini
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, SP, Brazil
    Chem Res Toxicol 15:506-11. 2002
    ..Overall, the results support a role for peroxynitrite and its derived radicals in the tissue pathology associated with inflammatory conditions...
  91. ncbi request reprint Inducible nitric oxide synthase is required in alcohol-induced liver injury: studies with knockout mice
    Stephen E McKim
    Department of Pharmacology, University of North Carolina, Chapel Hill, USA
    Gastroenterology 125:1834-44. 2003
    ..However, the production of the free radical nitric oxide (NO*) by inducible nitric oxide synthase (iNOS) could also be involved...
  92. ncbi request reprint Electron spin resonance and spin trapping technique provide direct evidence that edaravone prevents acute ischemia-reperfusion injury of the liver by limiting free radical-mediated tissue damage
    Hiroshi Kono
    First Department of Surgery, University of Yamanashi, Yamanashi, Japan
    Free Radic Res 40:579-88. 2006
    ..These results demonstrate that edaravone is an effective blocker of free radicals in vivo in the liver after ischemia/reperfusion, leading to prevention of organ injury by limiting the deleterious effects of free radicals...
  93. ncbi request reprint In vivo identification of aflatoxin-induced free radicals in rat bile
    Rheal A Towner
    Free Radical Biology and Aging Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA
    Free Radic Biol Med 35:1330-40. 2003
    ..Trapped 4-POBN radical adducts were also detected in rat bile following the in vivo metabolism of aflatoxin-M1, one of the hydroxylated metabolites of AFB1...
  94. ncbi request reprint Reaction of human hemoglobin with peroxynitrite. Isomerization to nitrate and secondary formation of protein radicals
    Natalia Romero
    Departamento de Bioquimica, Facultad de Medicina, 11800 Montevideo, Uruguay
    J Biol Chem 278:44049-57. 2003
    ..The results obtained support a role of oxyhemoglobin as a relevant intravascular sink of peroxynitrite...
  95. ncbi request reprint Prevention of hepatic ischemia-reperfusion injury by green tea extract
    Zhi Zhong
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, 27599, USA
    Am J Physiol Gastrointest Liver Physiol 283:G957-64. 2002
    ..Therefore, GTE could prove to be effective in decreasing hepatic injury in disease states where ischemia-reperfusion occurs...
  96. pmc The role of thiol and nitrosothiol compounds in the nitric oxide-forming reactions of the iron-N-methyl-d-glucamine dithiocarbamate complex
    Koichiro Tsuchiya
    Department of Pharmacology, University of Tokushima School of Medicine, Tokushima 770 8503, Japan
    Biochem J 367:771-9. 2002
    ..The present study elucidates the difficulties of utilizing the (MGD)(2)Fe(2+) complex for the quantification of NO in biological samples, especially in vivo...