R G Martin

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Autoactivation of the marRAB multiple antibiotic resistance operon by the MarA transcriptional activator in Escherichia coli
    R G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892 0560, USA
    J Bacteriol 178:2216-23. 1996
  2. pmc Detection of low-level promoter activity within open reading frame sequences of Escherichia coli
    Mitsuoki Kawano
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Building 18T, Room 101, Bethesda, MD 20892 5430, USA
    Nucleic Acids Res 33:6268-76. 2005
  3. pmc Promoter discrimination at class I MarA regulon promoters mediated by glutamic acid 89 of the MarA transcriptional activator of Escherichia coli
    Robert G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD 20892 0560, USA
    J Bacteriol 193:506-15. 2011
  4. ncbi request reprint Genomics of the marA/soxS/rob regulon of Escherichia coli: identification of directly activated promoters by application of molecular genetics and informatics to microarray data
    Robert G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases NIH, Bethesda, MD 20892 0560, USA
    Mol Microbiol 44:1611-24. 2002
  5. ncbi request reprint Transcriptional and translational regulation of the marRAB multiple antibiotic resistance operon in Escherichia coli
    Robert G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bldg 5, Rm 333, National Institutes of Health, Bethesda, MD 20892 0560, USA
    Mol Microbiol 53:183-91. 2004
  6. ncbi request reprint The AraC transcriptional activators
    R G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0560, USA
    Curr Opin Microbiol 4:132-7. 2001
  7. ncbi request reprint Promoter discrimination by the related transcriptional activators MarA and SoxS: differential regulation by differential binding
    R G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, Bldg 5, Rm 333, N I H, Bethesda, MD 20892 0560, USA
    Mol Microbiol 35:623-34. 2000
  8. ncbi request reprint Structural requirements for marbox function in transcriptional activation of mar/sox/rob regulon promoters in Escherichia coli: sequence, orientation and spatial relationship to the core promoter
    R G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, Bldg 5, Rm 333, NIH, Bethesda, MD 20892 0560, USA
    Mol Microbiol 34:431-41. 1999
  9. pmc Fis, an accessorial factor for transcriptional activation of the mar (multiple antibiotic resistance) promoter of Escherichia coli in the presence of the activator MarA, SoxS, or Rob
    R G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892 0560, USA
    J Bacteriol 179:7410-9. 1997
  10. pmc Activation of the Escherichia coli marA/soxS/rob regulon in response to transcriptional activator concentration
    Robert G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0560, USA
    J Mol Biol 380:278-84. 2008

Collaborators

Detail Information

Publications21

  1. pmc Autoactivation of the marRAB multiple antibiotic resistance operon by the MarA transcriptional activator in Escherichia coli
    R G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892 0560, USA
    J Bacteriol 178:2216-23. 1996
    ..Thus, transcription of the marRAB operon is autorepressed by MarR and autoactivated by MarA at a site that also can be activated by SoxS...
  2. pmc Detection of low-level promoter activity within open reading frame sequences of Escherichia coli
    Mitsuoki Kawano
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Building 18T, Room 101, Bethesda, MD 20892 5430, USA
    Nucleic Acids Res 33:6268-76. 2005
    ..coli chromosome carries numerous -35 and -10 sequences with weak promoter activity but that most are not productively expressed because other features needed to enhance promoter activity and transcript stability are absent...
  3. pmc Promoter discrimination at class I MarA regulon promoters mediated by glutamic acid 89 of the MarA transcriptional activator of Escherichia coli
    Robert G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD 20892 0560, USA
    J Bacteriol 193:506-15. 2011
    ..We present a model which attributes the differences in binding and activation to the interference between the β- and γ-carbons of the amino acid at position 89 and the phosphate group between positions 12 and 13...
  4. ncbi request reprint Genomics of the marA/soxS/rob regulon of Escherichia coli: identification of directly activated promoters by application of molecular genetics and informatics to microarray data
    Robert G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases NIH, Bethesda, MD 20892 0560, USA
    Mol Microbiol 44:1611-24. 2002
    ..We estimate that the total number of directly activated promoters in the regulon is less than 40...
  5. ncbi request reprint Transcriptional and translational regulation of the marRAB multiple antibiotic resistance operon in Escherichia coli
    Robert G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bldg 5, Rm 333, National Institutes of Health, Bethesda, MD 20892 0560, USA
    Mol Microbiol 53:183-91. 2004
    ..Increasing the 5 bp spacing between the SD sequence and the GTG codon by one to four bases reduced the translational efficiency by 50-75%. Inefficient translation of marR may help to sensitize the cell to environmental signals...
  6. ncbi request reprint The AraC transcriptional activators
    R G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0560, USA
    Curr Opin Microbiol 4:132-7. 2001
    ..Surprisingly, the virulence activator, Rns, must bind to both upstream and downstream sites in order to activate the rns promoter...
  7. ncbi request reprint Promoter discrimination by the related transcriptional activators MarA and SoxS: differential regulation by differential binding
    R G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, Bldg 5, Rm 333, N I H, Bethesda, MD 20892 0560, USA
    Mol Microbiol 35:623-34. 2000
    ..Discrimination enables the cell to customize its response to the stresses that trigger synthesis of the activators...
  8. ncbi request reprint Structural requirements for marbox function in transcriptional activation of mar/sox/rob regulon promoters in Escherichia coli: sequence, orientation and spatial relationship to the core promoter
    R G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, Bldg 5, Rm 333, NIH, Bethesda, MD 20892 0560, USA
    Mol Microbiol 34:431-41. 1999
    ..Marbox orientation and the spacing between marbox and signals for RNP binding are critical for transcriptional activation, presumably to align MarA with RNP...
  9. pmc Fis, an accessorial factor for transcriptional activation of the mar (multiple antibiotic resistance) promoter of Escherichia coli in the presence of the activator MarA, SoxS, or Rob
    R G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892 0560, USA
    J Bacteriol 179:7410-9. 1997
    ..Thus, Fis acts as an accessory transcriptional activator at the mar promoter...
  10. pmc Activation of the Escherichia coli marA/soxS/rob regulon in response to transcriptional activator concentration
    Robert G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0560, USA
    J Mol Biol 380:278-84. 2008
    ..Thus, the concentration and nature of activator determine which regulon promoters are activated, as well as the extent of their activation...
  11. ncbi request reprint Complex formation between activator and RNA polymerase as the basis for transcriptional activation by MarA and SoxS in Escherichia coli
    Robert G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0560, USA
    Mol Microbiol 43:355-70. 2002
    ..We suggest that activator-RNP complexes scan the chromosome and bind promoters of the regulon more efficiently than either RNP or the activators alone...
  12. ncbi request reprint Probing the Escherichia coli transcriptional activator MarA using alanine-scanning mutagenesis: residues important for DNA binding and activation
    W K Gillette
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892 0560, USA
    J Mol Biol 299:1245-55. 2000
    ..Furthermore, the important role of phosphate contacts in marbox affinity suggests that indirect readout contributes to binding site recognition by MarA...
  13. pmc Regulation of the multiple antibiotic resistance (mar) regulon by marORA sequences in Escherichia coli
    R G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892 0560, USA
    J Bacteriol 177:4176-8. 1995
    ....
  14. pmc A novel DNA-binding motif in MarA: the first structure for an AraC family transcriptional activator
    S Rhee
    Laboratory of Molecular Biology, National Institutes of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0560, USA
    Proc Natl Acad Sci U S A 95:10413-8. 1998
    ..Extensive interactions between the recognition helices and the DNA major groove provide the sequence specificity...
  15. ncbi request reprint Structure and dynamics of MarA-DNA complexes: an NMR investigation
    B Dangi
    Laboratory of Chemical Physics, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0560, USA
    J Mol Biol 314:113-27. 2001
    ....
  16. pmc Transcriptional activation by MarA, SoxS and Rob of two tolC promoters using one binding site: a complex promoter configuration for tolC in Escherichia coli
    Aixia Zhang
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 0560, USA
    Mol Microbiol 69:1450-5. 2008
    ..The multiple tolC promoters may allow the cell to respond to diverse environments by co-ordinating tolC transcription with other appropriate functions...
  17. pmc Binding of purified multiple antibiotic-resistance repressor protein (MarR) to mar operator sequences
    R G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 92:5456-60. 1995
    ..Salicylate appears to induce the mar operon by binding to MarR and inhibiting complex formation, whereas tetracycline and chloramphenicol, which neither bind MarR nor inhibit complex formation, must induce by an indirect mechanism...
  18. ncbi request reprint Analysis of microarray data for the marA, soxS, and rob regulons of Escherichia coli
    Robert G Martin
    Laboratory of Molecular Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 0560, USA
    Methods Enzymol 370:278-80. 2003
  19. ncbi request reprint Versatility of the carboxy-terminal domain of the alpha subunit of RNA polymerase in transcriptional activation: use of the DNA contact site as a protein contact site for MarA
    Bindi Dangi
    Laboratory of Chemical Physics, National Institute of Diabetes, Digestive and Kidney Diseases, National Institute of Health, Bethesda, MD 20892, USA
    Mol Microbiol 54:45-59. 2004
    ..These results reveal a new versatility for alpha-CTD in transcriptional activation...
  20. pmc An excretory function for the Escherichia coli outer membrane pore TolC: upregulation of marA and soxS transcription and Rob activity due to metabolites accumulated in tolC mutants
    Judah L Rosner
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0560, USA
    J Bacteriol 191:5283-92. 2009
    ..As these metabolites accumulate during growth, they trigger the upregulation of MarA, SoxS, and Rob, which in turn upregulate tolC and help rid the bacteria of these metabolites, thereby restoring homeostasis...
  21. pmc Posttranscriptional activation of the transcriptional activator Rob by dipyridyl in Escherichia coli
    Judah L Rosner
    Laboratory of Molecular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bldg 5 Rm 333, Bethesda, MD 20892 0560, USA
    J Bacteriol 184:1407-16. 2002
    ..Thus, it appears that Rob can be converted from a low activity state to a high-activity state by a CTD-mediated mechanism in vivo or by purification in vitro...