David H Margulies

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc How the virus outsmarts the host: function and structure of cytomegalovirus MHC-I-like molecules in the evasion of natural killer cell surveillance
    Maria Jamela Revilleza
    Molecular Biology Section, Laboratory of Immunology, NIAID, National Institutes of Health, Bethesda, MD 20892 1892, USA
    J Biomed Biotechnol 2011:724607. 2011
  2. ncbi request reprint Monoclonal antibodies: producing magic bullets by somatic cell hybridization
    David H Margulies
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 10 Center Drive, MSC 1892, Building 10, Room 11N311, Bethesda, MD 20892 1892, USA
    J Immunol 174:2451-2. 2005
  3. pmc CD28, costimulator or agonist receptor?
    David H Margulies
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, Building 10, Room 11N311, Bethesda, MD 20892, USA
    J Exp Med 197:949-53. 2003
  4. doi request reprint Home schooling of NK cells
    David H Margulies
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 1892, USA
    Immunity 30:313-5. 2009
  5. pmc Structural basis of mouse cytomegalovirus m152/gp40 interaction with RAE1γ reveals a paradigm for MHC/MHC interaction in immune evasion
    Rui Wang
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 109:E3578-87. 2012
  6. pmc A structural and molecular dynamics approach to understanding the peptide-receptive transition state of MHC-I molecules
    Michael G Mage
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases NIAID, NIH, Bethesda, MD 20892 1892, USA
    Mol Immunol 55:123-5. 2013
  7. pmc Direct interaction of the mouse cytomegalovirus m152/gp40 immunoevasin with RAE-1 isoforms
    Li Zhi
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 1892, USA
    Biochemistry 49:2443-53. 2010
  8. pmc Crystal structure of the murine cytomegalovirus MHC-I homolog m144
    Kannan Natarajan
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 1892, USA
    J Mol Biol 358:157-71. 2006
  9. pmc Estimation of low frequency antigen-presenting cells with a novel RELISPOT assay
    Amiran K Dzutsev
    Vaccine Branch, CCR, NCI, NIH, Bethesda, MD 20892 1578, USA
    J Immunol Methods 333:71-8. 2008
  10. ncbi request reprint Role of alpha3 domain of class I MHC molecules in the activation of high- and low-avidity CD8+ CTLs
    Igor M Belyakov
    Molecular Immunogenetics and Vaccine Research Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Int Immunol 19:1413-20. 2007

Collaborators

Detail Information

Publications28

  1. pmc How the virus outsmarts the host: function and structure of cytomegalovirus MHC-I-like molecules in the evasion of natural killer cell surveillance
    Maria Jamela Revilleza
    Molecular Biology Section, Laboratory of Immunology, NIAID, National Institutes of Health, Bethesda, MD 20892 1892, USA
    J Biomed Biotechnol 2011:724607. 2011
    ..In this review, we explore the function, structure, and evolution of MHC-I-like molecules of the CMVs and speculate on the dynamic development of novel immunoevasive functions based on the MHC-I protein fold...
  2. ncbi request reprint Monoclonal antibodies: producing magic bullets by somatic cell hybridization
    David H Margulies
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 10 Center Drive, MSC 1892, Building 10, Room 11N311, Bethesda, MD 20892 1892, USA
    J Immunol 174:2451-2. 2005
  3. pmc CD28, costimulator or agonist receptor?
    David H Margulies
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, Building 10, Room 11N311, Bethesda, MD 20892, USA
    J Exp Med 197:949-53. 2003
  4. doi request reprint Home schooling of NK cells
    David H Margulies
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 1892, USA
    Immunity 30:313-5. 2009
    ..In this issue of Immunity, Chalifour et al. (2009) show that for natural killer (NK) cells to achieve their full effector potential, NK inhibitory receptors must developmentally interact with MHC-I ligands expressed in cis...
  5. pmc Structural basis of mouse cytomegalovirus m152/gp40 interaction with RAE1γ reveals a paradigm for MHC/MHC interaction in immune evasion
    Rui Wang
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 109:E3578-87. 2012
    ..This structure of an MHC-I-like immunoevasin/MHC-I-like ligand complex explains the binding specificity of m152 for RAE1 and allows modeling of the interaction of m152 with classical MHC-I and of related viral immunoevasins...
  6. pmc A structural and molecular dynamics approach to understanding the peptide-receptive transition state of MHC-I molecules
    Michael G Mage
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases NIAID, NIH, Bethesda, MD 20892 1892, USA
    Mol Immunol 55:123-5. 2013
    ....
  7. pmc Direct interaction of the mouse cytomegalovirus m152/gp40 immunoevasin with RAE-1 isoforms
    Li Zhi
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 1892, USA
    Biochemistry 49:2443-53. 2010
    ..Molecular modeling of the different RAE-1 isoforms suggests a potential site for the m152 interaction...
  8. pmc Crystal structure of the murine cytomegalovirus MHC-I homolog m144
    Kannan Natarajan
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 1892, USA
    J Mol Biol 358:157-71. 2006
    ..A region of 13 amino acid residues, corresponding to the amino-terminal portion of the alpha2 helix, is missing in the electron density map, suggesting an area of structural flexibility that may be involved in ligand binding...
  9. pmc Estimation of low frequency antigen-presenting cells with a novel RELISPOT assay
    Amiran K Dzutsev
    Vaccine Branch, CCR, NCI, NIH, Bethesda, MD 20892 1578, USA
    J Immunol Methods 333:71-8. 2008
    ..Using this RELISPOT (Rosette ELISPOT) method we demonstrate the dynamic interplay between CD8 T cells and professional and non-professional APCs following virus challenge...
  10. ncbi request reprint Role of alpha3 domain of class I MHC molecules in the activation of high- and low-avidity CD8+ CTLs
    Igor M Belyakov
    Molecular Immunogenetics and Vaccine Research Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Int Immunol 19:1413-20. 2007
    ..Thus, low-avidity CTL may not be able to take advantage of the interaction between CD8 and the alpha3 domain of non-presenting class I MHC molecules, perhaps because of a shorter dwell time for the TCR-MHC interaction...
  11. ncbi request reprint MHC class I recognition by Ly49 natural killer cell receptors
    Kannan Natarajan
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 1892, USA
    Mol Immunol 38:1023-7. 2002
    ..We also discuss results from recent mutagenesis studies of the Ly49A/H-2D(d) interaction in the context of the complex structure...
  12. pmc Different vaccine vectors delivering the same antigen elicit CD8+ T cell responses with distinct clonotype and epitope specificity
    Mitsuo Honda
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 183:2425-34. 2009
    ..These findings suggest that different gene-based vectors generate peptides with alternative conformations within MHC-I that elicit distinct T cell responses after vaccination...
  13. pmc The peptide-receptive transition state of MHC class I molecules: insight from structure and molecular dynamics
    Michael G Mage
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 189:1391-9. 2012
    ....
  14. ncbi request reprint Structure and function of natural killer cell receptors: multiple molecular solutions to self, nonself discrimination
    Kannan Natarajan
    Molecular Biology Section, Laboratory of Immunology, NIAID, NIH, Bethesda, Maryland 20892 1892, USA
    Annu Rev Immunol 20:853-85. 2002
    ....
  15. pmc Cellular expression and crystal structure of the murine cytomegalovirus major histocompatibility complex class I-like glycoprotein, m153
    Janet Mans
    Molecular Biology Section, Laboratory of Immunology, NIAID, National Institutes of Health, Bethesda, Maryland 20892 1892, USA
    J Biol Chem 282:35247-58. 2007
    ..The structure provides a useful framework for comparative analysis of the divergent members of the m145 family...
  16. pmc Structural basis of the CD8 alpha beta/MHC class I interaction: focused recognition orients CD8 beta to a T cell proximal position
    Rui Wang
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 183:2554-64. 2009
    ....
  17. pmc A single residue, arginine 65, is critical for the functional interaction of leukocyte-associated inhibitory receptor-1 with collagens
    Xiaobin Tang
    Receptor Cell Biology Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA
    J Immunol 182:5446-52. 2009
    ..Likewise, LAIR-1 R65K protein has decreased avidity for cells expressing transmembrane collagen XVII. Thus, a single residue, Arg65, is critical for the interaction of LAIR-1 with collagens...
  18. pmc Structure and function of murine cytomegalovirus MHC-I-like molecules: how the virus turned the host defense to its advantage
    Janet Mans
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bldg 10 Room 11N311, 10 Center Drive, Bethesda, MD 20892 1892, USA
    Immunol Res 43:264-79. 2009
    ....
  19. ncbi request reprint Avidity of CD8 T cells sharpens immunodominance
    Amiran H Dzutsev
    Vaccine Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 1578, USA
    Int Immunol 19:497-507. 2007
    ..Since most self-reactive T cells of high avidity are depleted in the thymus, the selection of immunodominant epitopes based on the expansion of high-avidity T cells in the periphery reduces the potential for autoimmunity...
  20. ncbi request reprint Binding of the natural killer cell inhibitory receptor Ly49A to its major histocompatibility complex class I ligand. Crucial contacts include both H-2Dd AND beta 2-microglobulin
    Jian Wang
    Molecular Biology Section, Laboratory of Immunology, NIAID, National Institutes of Health, Bethesda, Maryland 20892 1892, USA
    J Biol Chem 277:1433-42. 2002
    ..H-2D(d) interface at Site 2 includes a network of water molecules, suggesting a molecular basis for allelic specificity in natural killer cell recognition...
  21. ncbi request reprint A serine/threonine phosphorylation site in the ectodomain of a T cell receptor beta chain is required for activation by superantigen
    Dmitriy E Lukashev
    Biochemistry and Immunopharmacology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Recept Signal Transduct Res 23:33-52. 2003
    ..These results, interpreted in the context of the known three-dimensional structure of the complex of SEB and TCR, are consistent with the view that serine-88 is important for the contact of the TCR beta chain with SEB...
  22. pmc The TLR3 signaling complex forms by cooperative receptor dimerization
    Joshua N Leonard
    Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 105:258-63. 2008
    ..We conclude that TLR3 assembles on dsRNA as stable dimers and that the minimal signaling unit is one TLR3 dimer...
  23. ncbi request reprint Molecular interactions: stiff or floppy (or somewhere in between?)
    David H Margulies
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, Building 10, Room 11N311, 10 Center Drive, Bethesda, MD 20892, USA
    Immunity 19:772-4. 2003
    ..This contrasts with "induced fit" that accounts for TCR adaptation to multiple MHCp ligands...
  24. doi request reprint Abacavir induces loading of novel self-peptides into HLA-B*57: 01: an autoimmune model for HLA-associated drug hypersensitivity
    Michael A Norcross
    Laboratory of Immunology, Division of Therapeutic Proteins, Office of Biotechnology Products, Center for Drug Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    AIDS 26:F21-9. 2012
    ....
  25. ncbi request reprint Spontaneous organ-specific Th2-mediated autoimmunity in TCR transgenic mice
    Sophie Candon
    Molecular Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA
    J Immunol 172:2917-24. 2004
    ..This in vivo model of spontaneous Th2-mediated, organ-specific autoimmunity provides a unique example in which the clonotypic TCR conveys the Th2 disease phenotype...
  26. ncbi request reprint Variable MHC class I engagement by Ly49 natural killer cell receptors demonstrated by the crystal structure of Ly49C bound to H-2K(b)
    Julie Dam
    Center for Advanced Research in Biotechnology, W M Keck Laboratory for Structural Biology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA
    Nat Immunol 4:1213-22. 2003
    ..We propose a dynamic model for Ly49-MHC class I interactions involving conformational changes in the receptor, whereby variations in Ly49 dimerization mediate different MHC-binding modes...
  27. ncbi request reprint Crystal structure of the Ly49I natural killer cell receptor reveals variability in dimerization mode within the Ly49 family
    Nazzareno Dimasi
    W M Keck Laboratory for Structural Biology, Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    J Mol Biol 320:573-85. 2002
    ..Such variability may enable certain Ly49 receptors, like Ly49I, to bind MHC-I molecules bivalently, thereby stabilizing receptor-ligand interactions and enhancing signal transmission to the NK cell...
  28. ncbi request reprint Backbone and side chain resonance assignmentsof a TRAV14-3 mouse T cell receptor domain
    Jin Shan Hu
    J Biomol NMR 31:271-2. 2005