L B Margolis

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Cytokines--strategic weapons in germ warfare?
    Leonid Margolis
    Section of Intercellular Interactions, Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Nat Biotechnol 21:15-6. 2003
  2. pmc Exploiting the anti-HIV-1 activity of acyclovir: suppression of primary and drug-resistant HIV isolates and potentiation of the activity by ribavirin
    Christophe Vanpouille
    Eunice Kennedy Shriver National Institute of Child Health and Human Development, Program of Physical Biology, National Institutes of Health, Bethesda, Maryland, USA
    Antimicrob Agents Chemother 56:2604-11. 2012
  3. pmc Selective transmission of R5 HIV-1 variants: where is the gatekeeper?
    Jean Charles Grivel
    Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, USA
    J Transl Med 9:S6. 2011
  4. ncbi request reprint Lymphocyte trafficking and HIV infection of human lymphoid tissue in a rotating wall vessel bioreactor
    L B Margolis
    NASA NIH Center for Three Dimensional Tissue Cultures, Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    AIDS Res Hum Retroviruses 13:1411-20. 1997
  5. ncbi request reprint Selective transmission of CCR5-utilizing HIV-1: the 'gatekeeper' problem resolved?
    Leonid Margolis
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20895, USA
    Nat Rev Microbiol 4:312-7. 2006
  6. ncbi request reprint HIV: from molecular recognition to tissue pathogenesis
    L Margolis
    Laboratory of Molecular and Cellular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    FEBS Lett 433:5-8. 1998
  7. pmc Blockade of CC chemokine receptor 5 (CCR5)-tropic human immunodeficiency virus-1 replication in human lymphoid tissue by CC chemokines
    L B Margolis
    Laboratory of Molecular and Cellular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Invest 101:1876-80. 1998
  8. ncbi request reprint HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1
    Jean Charles Grivel
    National Institute of Child Health and Human Development, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
    AIDS 21:1263-72. 2007
  9. doi request reprint Toxoplasma gondii inhibits R5 HIV-1 replication in human lymphoid tissues ex vivo
    Atfa Sassi
    Section of Intercellular Interactions, Program of Physical Biology, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Microbes Infect 11:1106-13. 2009
  10. pmc Real-time PCR assay of individual human immunodeficiency virus type 1 variants in coinfected human lymphoid tissues
    Yoshinori Ito
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Microbiol 41:2126-31. 2003

Collaborators

  • J Balzarini
  • C McGuigan
  • Christophe Vanpouille
  • Robin J Shattock
  • F Kirchhoff
  • Ian McGowan
  • Dominique Schols
  • Felix Yarovinsky
  • Maxime Lecerf
  • R F Schinazi
  • H Golding
  • Matthias Gotte
  • Christine Goffinet
  • Patricia S Fletcher
  • Kurt Vermeire
  • Jean Charles Grivel
  • Andrea Lisco
  • Beda Brichacek
  • Elke Rücker
  • Angelique Biancotto
  • Jan Munch
  • Yoshinori Ito
  • Cristian Condack
  • Wendy Fitzgerald
  • Silvia S Chen
  • Atfa Sassi
  • Hacene Boukari
  • Julie Elliott
  • Peter Anton
  • Jacob M van Laar
  • Steffen Wildum
  • Massimo Alfano
  • Jeffrey D Lifson
  • Alan Sher
  • Sara Hieny
  • Sheila F Mahoney
  • Pamela Stratton
  • Ralph Nossal
  • Robert Gorelick
  • Egor P Tchesnokov
  • Marco Derudas
  • Emilie Fromentin
  • Randy Fischer
  • Patricia Devaux
  • Rozbeh Mohammadi
  • Peter E Lipsky
  • Atanas Koulov
  • Michael Schindler
  • Marc Melchers
  • Raghavan Chinnadurai
  • Jeffery W Kelly
  • Knut Adermann
  • Oliver T Keppler
  • Ludger Ständker
  • Pedro Cuevas Sánchez
  • Walther Mothes
  • Y K Onno Teng
  • Devi Rajan
  • Amrie C Grammer
  • Douglas M Fowler
  • Wolf Georg Forssmann
  • Guillermo Gimenez-Gallego
  • Anke Specht
  • Ferdinand C Breedveld
  • Boris van der Zouwen
  • Roberto Cattaneo
  • Silvia Ghezzi
  • Guido Poli
  • Davide Corti
  • Matteo Trimarchi
  • Patricia Reichelderfer
  • Yana Kiselyeva
  • Silvia Chen
  • Matthias Brenner
  • Jutta Eisemann
  • Monica Michelini
  • Sandra Papini
  • Roberto P Revoltella
  • Joshua Zimmerberg

Detail Information

Publications28

  1. ncbi request reprint Cytokines--strategic weapons in germ warfare?
    Leonid Margolis
    Section of Intercellular Interactions, Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Nat Biotechnol 21:15-6. 2003
  2. pmc Exploiting the anti-HIV-1 activity of acyclovir: suppression of primary and drug-resistant HIV isolates and potentiation of the activity by ribavirin
    Christophe Vanpouille
    Eunice Kennedy Shriver National Institute of Child Health and Human Development, Program of Physical Biology, National Institutes of Health, Bethesda, Maryland, USA
    Antimicrob Agents Chemother 56:2604-11. 2012
    ....
  3. pmc Selective transmission of R5 HIV-1 variants: where is the gatekeeper?
    Jean Charles Grivel
    Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, USA
    J Transl Med 9:S6. 2011
    ..Knowledge of the gatekeepers' localization and function may enable us to enhance existing barriers against R5 transmission and to erect the new ones against all HIV-1 variants...
  4. ncbi request reprint Lymphocyte trafficking and HIV infection of human lymphoid tissue in a rotating wall vessel bioreactor
    L B Margolis
    NASA NIH Center for Three Dimensional Tissue Cultures, Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    AIDS Res Hum Retroviruses 13:1411-20. 1997
    ....
  5. ncbi request reprint Selective transmission of CCR5-utilizing HIV-1: the 'gatekeeper' problem resolved?
    Leonid Margolis
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20895, USA
    Nat Rev Microbiol 4:312-7. 2006
    ..Here, we propose that there is no single gatekeeper and that, instead, the selective transmission of R5 HIV-1 depends on the superimposition of multiple imperfect gatekeepers...
  6. ncbi request reprint HIV: from molecular recognition to tissue pathogenesis
    L Margolis
    Laboratory of Molecular and Cellular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    FEBS Lett 433:5-8. 1998
    ..Here, we discuss how the new molecular findings might relate to HIV pathogenesis in cells and tissues...
  7. pmc Blockade of CC chemokine receptor 5 (CCR5)-tropic human immunodeficiency virus-1 replication in human lymphoid tissue by CC chemokines
    L B Margolis
    Laboratory of Molecular and Cellular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Invest 101:1876-80. 1998
    ..Such an upregulation may contribute to the virus phenotype shift in the course of HIV disease in vivo...
  8. ncbi request reprint HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1
    Jean Charles Grivel
    National Institute of Child Health and Human Development, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
    AIDS 21:1263-72. 2007
    ....
  9. doi request reprint Toxoplasma gondii inhibits R5 HIV-1 replication in human lymphoid tissues ex vivo
    Atfa Sassi
    Section of Intercellular Interactions, Program of Physical Biology, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Microbes Infect 11:1106-13. 2009
    ..These ex vivo findings raise the possibility that, in addition to being a co-factor in HIV disease, T. gondii may influence the outcome of viral infection by preferentially suppressing R5 variants...
  10. pmc Real-time PCR assay of individual human immunodeficiency virus type 1 variants in coinfected human lymphoid tissues
    Yoshinori Ito
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Microbiol 41:2126-31. 2003
    ..The assay we developed may facilitate study of the mechanisms of the R5-to-X4 switch in viral swarms in human tissues infected with HIV-1...
  11. pmc A new class of dual-targeted antivirals: monophosphorylated acyclovir prodrug derivatives suppress both human immunodeficiency virus type 1 and herpes simplex virus type 2
    Christophe Vanpouille
    Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
    J Infect Dis 201:635-43. 2010
    ..However, both antiviral activities of acyclovir are dependent on phosphorylation by the nucleoside kinase activity of coinfecting human herpesviruses...
  12. ncbi request reprint CXCR4-tropic HIV-1 suppresses replication of CCR5-tropic HIV-1 in human lymphoid tissue by selective induction of CC-chemokines
    Yoshinori Ito
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Infect Dis 189:506-14. 2004
    ..Tissue-specific chemokine/cytokine network modulations triggered by individual HIV-1 variants may be an important mechanism of local interactions among HIV-1 quasi species in infected tissue...
  13. doi request reprint Acyclovir: a new use for an old drug
    Christophe Vanpouille
    Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Opin Infect Dis 22:583-7. 2009
    ..The controversial results of these trials are reviewed below in light of the recent discovery of the direct suppression of HIV-1 by ACV...
  14. doi request reprint War and peace between microbes: HIV-1 interactions with coinfecting viruses
    Andrea Lisco
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Host Microbe 6:403-8. 2009
    ..Understanding these phenomena may lead to anti-HIV-1 strategies that specifically target interactions between HIV-1 and coinfecting viruses...
  15. doi request reprint Acyclovir is activated into a HIV-1 reverse transcriptase inhibitor in herpesvirus-infected human tissues
    Andrea Lisco
    Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Host Microbe 4:260-70. 2008
    ..These data suggest that ACV anti-HIV-1 activity may contribute to the response of HIV/HHV-coinfected patients to ACV treatment and could guide strategies for the development of new HIV-1 RT inhibitors...
  16. pmc Movements of HIV-virions in human cervical mucus
    Hacene Boukari
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    Biomacromolecules 10:2482-8. 2009
    ..Changes in the microenvironments due to slow structural changes may facilitate movement of the virions, allowing them to reach the epithelial layer...
  17. pmc Use of human tissue explants to study human infectious agents
    Jean Charles Grivel
    Section of Intercellular Interactions, Program on Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, 10 Centre Drive, Room 9D58, Bethesda, Maryland 20892, USA
    Nat Protoc 4:256-69. 2009
    ..Using this protocol, culture and infections can be set up in 3-6 h and be maintained for 2-3 weeks, depending on the tissue used...
  18. doi request reprint A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24
    Angelique Biancotto
    Section on Intercellular Interactions, Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    J Virol Methods 157:98-101. 2009
    ..This cytometric bead assay allows the detection of p24 concentrations over 4 orders of magnitude from less than 0.4pg to up to 20,000pgml(-1) in a volume of 50microl and can be combined with other measurements...
  19. pmc Sustained secretion of immunoglobulin by long-lived human tonsil plasma cells
    Jacob M van Laar
    B Cell Biology Group, National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, USA
    Am J Pathol 171:917-27. 2007
    ..Taken together, the present study demonstrates that human lymphoid tissue harbors a population of nonproliferating plasma cells that are dependent on an intact microenvironment for ongoing Ig secretion...
  20. ncbi request reprint Measles virus vaccine attenuation: suboptimal infection of lymphatic tissue and tropism alteration
    Cristian Condack
    Laboratory of Molecular and Cellular Biophysics, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    J Infect Dis 196:541-9. 2007
    ..SLAM-expressing T cells are more readily infected by all MV strains than are SLAM-expressing B cells. Thus, vaccine attenuation may be caused by tropism alteration in combination with suboptimal replication...
  21. ncbi request reprint Multilineage differentiation of rhesus monkey embryonic stem cells in three-dimensional culture systems
    Silvia S Chen
    NASA NIH Center for Three Dimensional Tissue Culture, Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development NICHD, NIH, Bethesda, Maryland 20892, USA
    Stem Cells 21:281-95. 2003
    ..Three-dimensional culture systems are a valuable tool for studying the mechanisms of these phenomena...
  22. pmc Vpr and Vpu are important for efficient human immunodeficiency virus type 1 replication and CD4+ T-cell depletion in human lymphoid tissue ex vivo
    Elke Rücker
    National Institute of Child Health, Building 10, Room 9D58, Bethesda, MD 20892, USA
    J Virol 78:12689-93. 2004
    ..Our results demonstrate that nef, vpr, and vpu are all essential for efficient viral spread in HLT, suggesting an important role in AIDS pathogenesis...
  23. ncbi request reprint Semen-derived amyloid fibrils drastically enhance HIV infection
    Jan Munch
    Institute of Virology, University Clinic of Ulm, 89081 Ulm, Germany
    Cell 131:1059-71. 2007
    ..Amyloidogenic PAP fragments are abundant in seminal fluid and boost semen-mediated enhancement of HIV infection. Thus, they may play an important role in sexual transmission of HIV and could represent new targets for its prevention...
  24. pmc A naturally occurring variation in the proline-rich region does not attenuate human immunodeficiency virus type 1 nef function
    Elke Rücker
    Department of Virology Universitätsklinikum, Albert Einstein Allee 11, 89081 Ulm, Germany
    J Virol 78:10197-201. 2004
    ..Curr. Biol. 11:1294-1299, 2001). Our results demonstrate that this variation in the proline-rich region does not significantly affect the functional activity of Nef or HIV-1 infectivity or replication...
  25. ncbi request reprint Pertussis toxin B-oligomer dissociates T cell activation and HIV replication in CD4 T cells released from infected lymphoid tissue
    Massimo Alfano
    AIDS Immunopathogenesis Unit, Department of Immunology and Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy
    AIDS 19:1007-14. 2005
    ..To investigate, in human lymphoid tissue infected with HIV-1 ex vivo, the immunostimulatory and HIV inhibitory properties of pertussis toxin B oligomer (PTX-B) and of the genetically modified non-toxic PT-9K/129G...
  26. pmc The nonnucleoside reverse transcriptase inhibitor UC-781 inhibits human immunodeficiency virus type 1 infection of human cervical tissue and dissemination by migratory cells
    Patricia Fletcher
    Division of Infectious Diseases, Cellular and Molecular Medicine, St George s, University of London, UK
    J Virol 79:11179-86. 2005
    ..The prolonged protection observed following pretreatment of both genital and lymphoid tissues with UC-781 suggests that this class of inhibitors may have unique advantages over other classes of potential microbicide candidates...
  27. ncbi request reprint Ex vivo culture of human colorectal tissue for the evaluation of candidate microbicides
    Patricia S Fletcher
    St George s, University of London, Cranmer Terrace, Tooting, London SW17 0RE, UK
    AIDS 20:1237-45. 2006
    ..Establishment of an in vitro model to evaluate rectal safety and the efficacy of microbicide candidates...
  28. ncbi request reprint Design and cellular kinetics of dansyl-labeled CADA derivatives with anti-HIV and CD4 receptor down-modulating activity
    Kurt Vermeire
    Rega Institute for Medical Research, Department of Microbiology and Immunology, Katholieke Universiteit Leuven, Leuven, Belgium
    Biochem Pharmacol 74:566-78. 2007
    ..These fluorescent CADA analogs with reversible CD4 down-regulating potency can now be applied in further studies on receptor modulation, and in the exploration of their potentials as preventive and therapeutic anti-HIV drugs...