R J Maraia

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Nonphosphorylated human La antigen interacts with nucleolin at nucleolar sites involved in rRNA biogenesis
    Robert V Intine
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, 6 Center Dr, Rm 416, Bethesda, MD 20892 2753, USA
    Mol Cell Biol 24:10894-904. 2004
  2. pmc Precursor-product discrimination by La protein during tRNA metabolism
    Mark A Bayfield
    Intramural Research Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
    Nat Struct Mol Biol 16:430-7. 2009
  3. pmc RNA polymerase III mutants in TFIIF╬▒-like C37 that cause terminator readthrough with no decrease in transcription output
    Keshab Rijal
    Intramural Research Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA
    Nucleic Acids Res 41:139-55. 2013
  4. pmc Comparative whole genome sequencing reveals phenotypic tRNA gene duplication in spontaneous Schizosaccharomyces pombe La mutants
    James R Iben
    Intramural Research Program on Genomics of Differentiation, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, University of Massachusetts Medical School, Worcester, MA, USA
    Nucleic Acids Res 39:4728-42. 2011
  5. pmc Point mutations in the Rpb9-homologous domain of Rpc11 that impair transcription termination by RNA polymerase III
    James R Iben
    Intramural Research Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Commissioned Corps, US Public Health Service, Bethesda, MD, USA 20892
    Nucleic Acids Res 39:6100-13. 2011
  6. pmc 3' processing of eukaryotic precursor tRNAs
    Richard J Maraia
    Intramural Research Program, Eunice Kennedy Shriver NationalInstitute of Child Health and Human Development, NationalInstitutes of Health, Bethesda, MD, USA
    Wiley Interdiscip Rev RNA 2:362-75. 2011
  7. pmc Recognition of nascent RNA by the human La antigen: conserved and divergent features of structure and function
    R J Maraia
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
    Mol Cell Biol 21:367-79. 2001
  8. ncbi request reprint La protein and its associated small nuclear and nucleolar precursor RNAs
    Richard J Maraia
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, USA
    Gene Expr 10:41-57. 2002
  9. ncbi request reprint The La protein-RNA complex surfaces
    Richard J Maraia
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, 31 Center Drive, 2A25, Bethesda, Maryland 20892, USA
    Mol Cell 21:149-52. 2006
  10. ncbi request reprint Control of transfer RNA maturation by phosphorylation of the human La antigen on serine 366
    R V Intine
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 6:339-48. 2000

Collaborators

  • Y Huang
  • Mark A Bayfield
  • G J Darlington
  • J L Goodier
  • Miroslav Dundr
  • Melvin L DePamphilis
  • E D Green
  • T M Lowe
  • H Fan
  • X Zhang
  • JACK KEENE
  • T Misteli
  • D Perrotti
  • Q Zhou
  • Robert J White
  • Shiv Is Grewal
  • Sailen Barik
  • Robert V Intine
  • James R Iben
  • Keshab Rijal
  • Jung Min Park
  • Ruiqing Yang
  • Amy N Russo
  • Monique W Bruinsma
  • Vira Bitko
  • Nanhai He
  • Nelson S Yee
  • Ken ichi Noma
  • Jennifer A Fairley
  • Elena I Schwartz
  • Rossana Trotta
  • M Hamada
  • Julie K Mazeika
  • Aneeshkumar G Arimbasseri
  • Sam Hasson
  • Luigi Martino
  • Maria R Conte
  • Samuel Hasson
  • Ellen L W Kittler
  • Maria L Zapp
  • Sergei A Gaidamakov
  • Jingwei Xie
  • Jonathan A Epstein
  • Daniel Ahmad
  • Dagmar Bacikova
  • Joowon Lee
  • Amanda K Crawford
  • Denise Rockwell
  • Guennadi Kozlov
  • Kalle Gehring
  • R V Intine
  • Kunxin Luo
  • Eunmee Hong
  • Nadine S Jahchan
  • Qiang Li
  • Alla Musiyenko
  • Kristin Lorent
  • Michael Pack
  • Weilong Gong
  • Amy C Dolan
  • Alex Grinberg
  • Paul E Love
  • Matthew J Kohn
  • Stacy Fuhrmann
  • Ipsita Mukherjee
  • Hugh P Cam
  • Minoru S Ko
  • Claire Vech
  • Niall S Kenneth
  • Theodoros Kantidakis
  • Alex Vassilev
  • Yingmin Zhao
  • Elena Schwartz
  • Yingxin Zhao
  • Clara Guerzoni
  • Michael A Caligiuri
  • Scott A Tenenbaum
  • Giorgia Santilli
  • Bruno Calabretta
  • Amy L Sakulich
  • Tatiana Vignudelli
  • Mike W Byrom
  • Luisa Pecorari
  • Olivia Candini
  • Lance P Ford
  • Silvia Goldoni
  • S B Koduru
  • E Pierstorff
  • L Phan
  • A L Sakulich

Detail Information

Publications37

  1. pmc Nonphosphorylated human La antigen interacts with nucleolin at nucleolar sites involved in rRNA biogenesis
    Robert V Intine
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, 6 Center Dr, Rm 416, Bethesda, MD 20892 2753, USA
    Mol Cell Biol 24:10894-904. 2004
    ..The data suggest that interaction with nucleolin is, at least in part, responsible for nucleolar accumulation of La and that npLa may be involved in ribosome biogenesis...
  2. pmc Precursor-product discrimination by La protein during tRNA metabolism
    Mark A Bayfield
    Intramural Research Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
    Nat Struct Mol Biol 16:430-7. 2009
    ..Accordingly, the RRM1 mutations also impair an RNA chaperone activity of La. The results suggest how La distinguishes precursor from product RNAs, allowing it to recycle onto a new pre-tRNA...
  3. pmc RNA polymerase III mutants in TFIIF╬▒-like C37 that cause terminator readthrough with no decrease in transcription output
    Keshab Rijal
    Intramural Research Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA
    Nucleic Acids Res 41:139-55. 2013
    ..Surprisingly, multiple termination mutants have no decrease in transcript output relative to controls. These data are discussed in context of current models of pol III transcription...
  4. pmc Comparative whole genome sequencing reveals phenotypic tRNA gene duplication in spontaneous Schizosaccharomyces pombe La mutants
    James R Iben
    Intramural Research Program on Genomics of Differentiation, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, University of Massachusetts Medical School, Worcester, MA, USA
    Nucleic Acids Res 39:4728-42. 2011
    ..pombe mtDNA discrepancies. Thus, strain-specific and spontaneous phenotypic mutations can be mapped in S. pombe by Solexa sequencing...
  5. pmc Point mutations in the Rpb9-homologous domain of Rpc11 that impair transcription termination by RNA polymerase III
    James R Iben
    Intramural Research Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Commissioned Corps, US Public Health Service, Bethesda, MD, USA 20892
    Nucleic Acids Res 39:6100-13. 2011
    ..We propose that these C11 domains integrate action at the upper jaw and center of pol III during termination...
  6. pmc 3' processing of eukaryotic precursor tRNAs
    Richard J Maraia
    Intramural Research Program, Eunice Kennedy Shriver NationalInstitute of Child Health and Human Development, NationalInstitutes of Health, Bethesda, MD, USA
    Wiley Interdiscip Rev RNA 2:362-75. 2011
    ..A pathway is proposed in which 3' oligo(U) length is a primary determinant of La binding with subsequent steps distinguished by 3'-endo versus exo nucleases,chaperone activities, and nuclear surveillance...
  7. pmc Recognition of nascent RNA by the human La antigen: conserved and divergent features of structure and function
    R J Maraia
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
    Mol Cell Biol 21:367-79. 2001
  8. ncbi request reprint La protein and its associated small nuclear and nucleolar precursor RNAs
    Richard J Maraia
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, USA
    Gene Expr 10:41-57. 2002
    ..An imminent goal is to understand how the bipartite RNA binding, intracellular trafficking, and signal transduction activities of La are integrated with the maturation pathways of the various RNAs with which it associates...
  9. ncbi request reprint The La protein-RNA complex surfaces
    Richard J Maraia
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, 31 Center Drive, 2A25, Bethesda, Maryland 20892, USA
    Mol Cell 21:149-52. 2006
    ....
  10. ncbi request reprint Control of transfer RNA maturation by phosphorylation of the human La antigen on serine 366
    R V Intine
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 6:339-48. 2000
    ..Faithful phosphorylation of hLa on serine 366 reverses this block and promotes tRNA maturation. The results suggest that regulation of tRNA maturation at the level of RNase P cleavage may occur via phosphorylation of serine 366 of hLa...
  11. pmc Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human
    Y Huang
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, 6 Center Drive MSC 2753, Bethesda, MD 20892-2753, USA
    Nucleic Acids Res 29:2675-90. 2001
    ..The idea that the divergence in downstream TFIIIC subunits is associated with differences in pol III termination-related mechanisms that have been noted in the yeast and human systems but not reviewed previously is also considered...
  12. pmc Widespread use of TATA elements in the core promoters for RNA polymerases III, II, and I in fission yeast
    M Hamada
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2753, USA
    Mol Cell Biol 21:6870-81. 2001
    ....
  13. ncbi request reprint Isolation and cloning of four subunits of a fission yeast TFIIIC complex that includes an ortholog of the human regulatory protein TFIIICbeta
    Y Huang
    Laboratory of Molecular Growth Regulation, NICHD, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    J Biol Chem 275:31480-7. 2000
    ..pombe tRNA gene in vitro. These results establish a link between the downstream pol III TFs in yeast and humans...
  14. ncbi request reprint Phosphorylation of the human La antigen on serine 366 can regulate recycling of RNA polymerase III transcription complexes
    H Fan
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell 88:707-15. 1997
    ..This work demonstrates a novel mechanism of transcriptional control at the level of recycling of stable transcription complexes...
  15. ncbi request reprint Construction of FLAG and histidine tagging vectors for Schizosaccharomyces pombe
    Y Huang
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development NIH, Building 6, Room 3403, 6 Center Drive MSC 2753, Bethesda, MD 20892 2753, USA
    Yeast 18:463-8. 2001
    ..The function and applicability of these vectors were examined and the results are shown using the N-terminal tagging vector encoding Sfc6p, a subunit of the Sz. pombe RNA polymerase III general transcription factor, TFIIIC...
  16. pmc The conserved 7SK snRNA gene localizes to human chromosome 6 by homolog exclusion probing of somatic cell hybrid RNA
    C T Driscoll
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892
    Nucleic Acids Res 22:722-5. 1994
    ..The potential to map and subsequently isolate other small RNA genes by this approach and the use of homolog exclusion probing to discriminate among family members of highly related RNAs and DNAs in a single species is discussed...
  17. pmc Conservation of a masked nuclear export activity of La proteins and its effects on tRNA maturation
    Mark A Bayfield
    Intramural Research Program, National Institute of Child Health and Human Development, U S National Institutes of Health, Bethesda, MD 20892 2426, USA
    Mol Cell Biol 27:3303-12. 2007
    ....
  18. pmc The human Y4 small cytoplasmic RNA gene is controlled by upstream elements and resides on chromosome 7 with all other hY scRNA genes
    R J Maraia
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892
    Nucleic Acids Res 22:3045-52. 1994
    ..SCHs and chromosome 7-enriched YACs were used to demonstrate that all four hY RNA genes reside on human chromosome 7...
  19. ncbi request reprint Mouse and human La proteins differ in kinase substrate activity and activation mechanism for tRNA processing
    Jung Min Park
    Intramural Research Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2426, USA
    Gene Expr 14:71-81. 2007
    ..In closing, we note that species-specific signaling through La may be relevant to the La-dependent Mdm2 pathways of p53 metabolism and cancer progression in mice and humans...
  20. pmc CK2 is responsible for phosphorylation of human La protein serine-366 and can modulate rpL37 5'-terminal oligopyrimidine mRNA metabolism
    Elena I Schwartz
    Laboratory of Molecular Growth Regulation, NICHD, NIH, Bethesda, MD 20892 2753, USA
    Mol Cell Biol 24:9580-91. 2004
    ..Consistent with data that indicate that phosphorylation reverses negative effects of npLa on tRNA production, the present data suggest that CK2 phosphorylation of La can affect production of the translational machinery...
  21. ncbi request reprint Differential phosphorylation and subcellular localization of La RNPs associated with precursor tRNAs and translation-related mRNAs
    Robert V Intine
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Development, Bethesda, MD 20892, USA
    Mol Cell 12:1301-7. 2003
    ....
  22. pmc Mutations in the RNA polymerase III subunit Rpc11p that decrease RNA 3' cleavage activity increase 3'-terminal oligo(U) length and La-dependent tRNA processing
    Ying Huang
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, NIH, 31 Center Dr, Room 2A25, Bethesda, MD 20892 2426, USA
    Mol Cell Biol 25:621-36. 2005
    ..The data indicate that Rpc11p limits RNA 3'-U length and that this significantly restricts pre-tRNAs to a La-independent pathway of maturation in fission yeast...
  23. ncbi request reprint Aberrant nuclear trafficking of La protein leads to disordered processing of associated precursor tRNAs
    Robert V Intine
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Mol Cell 9:1113-23. 2002
    ..Instead, anomalous 5' and 3' end-containing, spliced tRNAs accumulate, complexed with the mutant La protein. Thus, appropriate nuclear trafficking by La affects the normal order of pre-tRNA processing...
  24. pmc A human Alu RNA-binding protein whose expression is associated with accumulation of small cytoplasmic Alu RNA
    D Y Chang
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, Bethesda, Maryland 20892
    Mol Cell Biol 14:3949-59. 1994
    ..The possibility that the primate-specific structure of this protein may have contributed to Alu evolution is considered...
  25. pmc The multifunctional RNA-binding protein La is required for mouse development and for the establishment of embryonic stem cells
    Jung Min Park
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, 31 Center Drive, Bldg 31, Rm 2A25, Bethesda, MD 20892 2426, USA
    Mol Cell Biol 26:1445-51. 2006
    ..The results indicate that in contrast to the situation in yeasts, La is essential in mammals and is one of a limited number of genes required as early as the development of the ICM...
  26. ncbi request reprint RNA polymerase III from the fission yeast, Schizosaccharomyces pombe
    Ying Huang
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    Methods Enzymol 370:165-73. 2003
  27. pmc The fission yeast TFIIB-related factor limits RNA polymerase III to a TATA-dependent pathway of TBP recruitment
    Ying Huang
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, USA
    Nucleic Acids Res 31:2108-16. 2003
    ..Since previous whole genome analysis also revealed TATA elements upstream of tRNA genes in Arabidopsis, this pathway may be more widespread than appreciated previously...
  28. pmc La-related protein 4 binds poly(A), interacts with the poly(A)-binding protein MLLE domain via a variant PAM2w motif, and can promote mRNA stability
    Ruiqing Yang
    31 Center Drive, Bldg 31, Room 2A25, Bethesda, MD 20892 2426, USA
    Mol Cell Biol 31:542-56. 2011
    ..We propose that LARP4 activity is integrated with other PAM2 protein activities by PABP as part of mRNA homeostasis...
  29. ncbi request reprint Separate RNA-binding surfaces on the multifunctional La protein mediate distinguishable activities in tRNA maturation
    Ying Huang
    Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, US National Institutes of Health, 31 Center Dr, Rm 2A25, Bethesda, Maryland 20892, USA
    Nat Struct Mol Biol 13:611-8. 2006
    ..The two activities of La in tRNA maturation map to its two conserved RNA-binding surfaces and suggest a modular model that has implications for its other ligands...
  30. ncbi request reprint BCR/ABL activates mdm2 mRNA translation via the La antigen
    Rossana Trotta
    Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson Medical College, Philadelphia, PA 19107, USA
    Cancer Cell 3:145-60. 2003
    ..By contrast, La overexpression led to increased MDM2 levels and enhanced resistance to apoptosis. Thus, La-dependent activation of mdm2 translation might represent an important molecular mechanism involved in BCR/ABL leukemogenesis...
  31. pmc Cellular La protein shields nonsegmented negative-strand RNA viral leader RNA from RIG-I and enhances virus growth by diverse mechanisms
    Vira Bitko
    Department of Biochemistry and Molecular Biology, University of South Alabama, College of Medicine, 307 University Blvd, Mobile, AL 36688 0002, USA
    J Virol 82:7977-87. 2008
    ....
  32. doi request reprint A La-related protein modulates 7SK snRNP integrity to suppress P-TEFb-dependent transcriptional elongation and tumorigenesis
    Nanhai He
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Cell 29:588-99. 2008
    ..Through PIP7S modulation of P-TEFb, our data thus link a general elongation factor to growth control and tumorigenesis...
  33. pmc Human La is found at RNA polymerase III-transcribed genes in vivo
    Jennifer A Fairley
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    Proc Natl Acad Sci U S A 102:18350-5. 2005
    ..However, gene occupancy depends on the phosphorylation status of La, with the less prevalent, unphosphorylated form being found selectively on pol III templates...
  34. pmc Mutation of RNA Pol III subunit rpc2/polr3b Leads to Deficiency of Subunit Rpc11 and disrupts zebrafish digestive development
    Nelson S Yee
    Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America
    PLoS Biol 5:e312. 2007
    ..These data show that functional interactions between Pol III subunits have been conserved during eukaryotic evolution and support the utility of zebrafish as a model vertebrate for analysis of Pol III function...
  35. ncbi request reprint A role for TFIIIC transcription factor complex in genome organization
    Ken ichi Noma
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 125:859-72. 2006
    ..Our analyses uncover a general genome organization mechanism involving conserved TFIIIC complex...
  36. doi request reprint It's a mod mod tRNA world
    Richard J Maraia
    Nat Chem Biol 4:162-4. 2008