Research Topics
| R J MaraiaSummaryAffiliation: National Institutes of Health Country: USA Publications
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Detail Information
Publications
Nonphosphorylated human La antigen interacts with nucleolin at nucleolar sites involved in rRNA biogenesisRobert V Intine
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, 6 Center Dr, Rm 416, Bethesda, MD 20892 2753, USA
Mol Cell Biol 24:10894-904. 2004..The data suggest that interaction with nucleolin is, at least in part, responsible for nucleolar accumulation of La and that npLa may be involved in ribosome biogenesis...- 3' processing of eukaryotic precursor tRNAsRichard J Maraia
Intramural Research Program, Eunice Kennedy Shriver NationalInstitute of Child Health and Human Development, NationalInstitutes of Health, Bethesda, MD, USA
Wiley Interdiscip Rev RNA 2:362-75. 2011..A pathway is proposed in which 3' oligo(U) length is a primary determinant of La binding with subsequent steps distinguished by 3'-endo versus exo nucleases,chaperone activities, and nuclear surveillance... 
The La protein-RNA complex surfacesRichard J Maraia
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, 31 Center Drive, 2A25, Bethesda, Maryland 20892, USA
Mol Cell 21:149-52. 2006....- La protein and its associated small nuclear and nucleolar precursor RNAsRichard J Maraia
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, USA
Gene Expr 10:41-57. 2002..An imminent goal is to understand how the bipartite RNA binding, intracellular trafficking, and signal transduction activities of La are integrated with the maturation pathways of the various RNAs with which it associates... - Recognition of nascent RNA by the human La antigen: conserved and divergent features of structure and functionR J Maraia
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
Mol Cell Biol 21:367-79. 2001 - Control of transfer RNA maturation by phosphorylation of the human La antigen on serine 366R V Intine
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Cell 6:339-48. 2000..Faithful phosphorylation of hLa on serine 366 reverses this block and promotes tRNA maturation. The results suggest that regulation of tRNA maturation at the level of RNase P cleavage may occur via phosphorylation of serine 366 of hLa... - Widespread use of TATA elements in the core promoters for RNA polymerases III, II, and I in fission yeastM Hamada
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2753, USA
Mol Cell Biol 21:6870-81. 2001.... - Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and humanY Huang
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, 6 Center Drive MSC 2753, Bethesda, MD 20892-2753, USA
Nucleic Acids Res 29:2675-90. 2001..The idea that the divergence in downstream TFIIIC subunits is associated with differences in pol III termination-related mechanisms that have been noted in the yeast and human systems but not reviewed previously is also considered... - Isolation and cloning of four subunits of a fission yeast TFIIIC complex that includes an ortholog of the human regulatory protein TFIIICbetaY Huang
Laboratory of Molecular Growth Regulation, NICHD, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
J Biol Chem 275:31480-7. 2000..pombe tRNA gene in vitro. These results establish a link between the downstream pol III TFs in yeast and humans... - Construction of FLAG and histidine tagging vectors for Schizosaccharomyces pombeY Huang
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development NIH, Building 6, Room 3403, 6 Center Drive MSC 2753, Bethesda, MD 20892 2753, USA
Yeast 18:463-8. 2001..The function and applicability of these vectors were examined and the results are shown using the N-terminal tagging vector encoding Sfc6p, a subunit of the Sz. pombe RNA polymerase III general transcription factor, TFIIIC... - The conserved 7SK snRNA gene localizes to human chromosome 6 by homolog exclusion probing of somatic cell hybrid RNAC T Driscoll
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892
Nucleic Acids Res 22:722-5. 1994..The potential to map and subsequently isolate other small RNA genes by this approach and the use of homolog exclusion probing to discriminate among family members of highly related RNAs and DNAs in a single species is discussed... - Phosphorylation of the human La antigen on serine 366 can regulate recycling of RNA polymerase III transcription complexesH Fan
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
Cell 88:707-15. 1997..This work demonstrates a novel mechanism of transcriptional control at the level of recycling of stable transcription complexes... - Precursor-product discrimination by La protein during tRNA metabolismMark A Bayfield
Intramural Research Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
Nat Struct Mol Biol 16:430-7. 2009..Accordingly, the RRM1 mutations also impair an RNA chaperone activity of La. The results suggest how La distinguishes precursor from product RNAs, allowing it to recycle onto a new pre-tRNA... - Conservation of a masked nuclear export activity of La proteins and its effects on tRNA maturationMark A Bayfield
Intramural Research Program, National Institute of Child Health and Human Development, U S National Institutes of Health, Bethesda, MD 20892 2426, USA
Mol Cell Biol 27:3303-12. 2007.... - The human Y4 small cytoplasmic RNA gene is controlled by upstream elements and resides on chromosome 7 with all other hY scRNA genesR J Maraia
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892
Nucleic Acids Res 22:3045-52. 1994..SCHs and chromosome 7-enriched YACs were used to demonstrate that all four hY RNA genes reside on human chromosome 7... - CK2 is responsible for phosphorylation of human La protein serine-366 and can modulate rpL37 5'-terminal oligopyrimidine mRNA metabolismElena I Schwartz
Laboratory of Molecular Growth Regulation, NICHD, NIH, Bethesda, MD 20892 2753, USA
Mol Cell Biol 24:9580-91. 2004..Consistent with data that indicate that phosphorylation reverses negative effects of npLa on tRNA production, the present data suggest that CK2 phosphorylation of La can affect production of the translational machinery... - Differential phosphorylation and subcellular localization of La RNPs associated with precursor tRNAs and translation-related mRNAsRobert V Intine
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Development, Bethesda, MD 20892, USA
Mol Cell 12:1301-7. 2003.... - Mouse and human La proteins differ in kinase substrate activity and activation mechanism for tRNA processingJung Min Park
Intramural Research Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2426, USA
Gene Expr 14:71-81. 2007..In closing, we note that species-specific signaling through La may be relevant to the La-dependent Mdm2 pathways of p53 metabolism and cancer progression in mice and humans... - Mutations in the RNA polymerase III subunit Rpc11p that decrease RNA 3' cleavage activity increase 3'-terminal oligo(U) length and La-dependent tRNA processingYing Huang
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, NIH, 31 Center Dr, Room 2A25, Bethesda, MD 20892 2426, USA
Mol Cell Biol 25:621-36. 2005..The data indicate that Rpc11p limits RNA 3'-U length and that this significantly restricts pre-tRNAs to a La-independent pathway of maturation in fission yeast... - Aberrant nuclear trafficking of La protein leads to disordered processing of associated precursor tRNAsRobert V Intine
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
Mol Cell 9:1113-23. 2002..Instead, anomalous 5' and 3' end-containing, spliced tRNAs accumulate, complexed with the mutant La protein. Thus, appropriate nuclear trafficking by La affects the normal order of pre-tRNA processing... - A human Alu RNA-binding protein whose expression is associated with accumulation of small cytoplasmic Alu RNAD Y Chang
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, Bethesda, Maryland 20892
Mol Cell Biol 14:3949-59. 1994..The possibility that the primate-specific structure of this protein may have contributed to Alu evolution is considered... - RNA polymerase III from the fission yeast, Schizosaccharomyces pombeYing Huang
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2753, USA
Methods Enzymol 370:165-73. 2003 - The multifunctional RNA-binding protein La is required for mouse development and for the establishment of embryonic stem cellsJung Min Park
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, 31 Center Drive, Bldg 31, Rm 2A25, Bethesda, MD 20892 2426, USA
Mol Cell Biol 26:1445-51. 2006..The results indicate that in contrast to the situation in yeasts, La is essential in mammals and is one of a limited number of genes required as early as the development of the ICM... - The fission yeast TFIIB-related factor limits RNA polymerase III to a TATA-dependent pathway of TBP recruitmentYing Huang
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, USA
Nucleic Acids Res 31:2108-16. 2003..Since previous whole genome analysis also revealed TATA elements upstream of tRNA genes in Arabidopsis, this pathway may be more widespread than appreciated previously... - La-related protein 4 binds poly(A), interacts with the poly(A)-binding protein MLLE domain via a variant PAM2w motif, and can promote mRNA stabilityRuiqing Yang
31 Center Drive, Bldg 31, Room 2A25, Bethesda, MD 20892 2426, USA
Mol Cell Biol 31:542-56. 2011..We propose that LARP4 activity is integrated with other PAM2 protein activities by PABP as part of mRNA homeostasis... - Separate RNA-binding surfaces on the multifunctional La protein mediate distinguishable activities in tRNA maturationYing Huang
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, US National Institutes of Health, 31 Center Dr, Rm. 2A25, Bethesda, Maryland 20892, USA
Nat Struct Mol Biol 13:611-8. 2006..The two activities of La in tRNA maturation map to its two conserved RNA-binding surfaces and suggest a modular model that has implications for its other ligands... - Cellular La protein shields nonsegmented negative-strand RNA viral leader RNA from RIG-I and enhances virus growth by diverse mechanismsVira Bitko
Department of Biochemistry and Molecular Biology, University of South Alabama, College of Medicine, 307 University Blvd, Mobile, AL 36688 0002, USA
J Virol 82:7977-87. 2008.... 
A La-related protein modulates 7SK snRNP integrity to suppress P-TEFb-dependent transcriptional elongation and tumorigenesisNanhai He
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
Mol Cell 29:588-99. 2008..Through PIP7S modulation of P-TEFb, our data thus link a general elongation factor to growth control and tumorigenesis...- Human La is found at RNA polymerase III-transcribed genes in vivoJennifer A Fairley
Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow G12 8QQ, United Kingdom
Proc Natl Acad Sci U S A 102:18350-5. 2005..However, gene occupancy depends on the phosphorylation status of La, with the less prevalent, unphosphorylated form being found selectively on pol III templates... - BCR/ABL activates mdm2 mRNA translation via the La antigenRossana Trotta
Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson Medical College, Philadelphia, PA 19107, USA
Cancer Cell 3:145-60. 2003..By contrast, La overexpression led to increased MDM2 levels and enhanced resistance to apoptosis. Thus, La-dependent activation of mdm2 translation might represent an important molecular mechanism involved in BCR/ABL leukemogenesis... - It's a mod mod tRNA worldRichard J Maraia
Nat Chem Biol 4:162-4. 2008 - Mutation of RNA Pol III subunit rpc2/polr3b Leads to Deficiency of Subunit Rpc11 and disrupts zebrafish digestive developmentNelson S Yee
Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America
PLoS Biol 5:e312. 2007..These data show that functional interactions between Pol III subunits have been conserved during eukaryotic evolution and support the utility of zebrafish as a model vertebrate for analysis of Pol III function... - A role for TFIIIC transcription factor complex in genome organizationKen ichi Noma
Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Cell 125:859-72. 2006..Our analyses uncover a general genome organization mechanism involving conserved TFIIIC complex...