Jennifer Lippincott-Schwartz

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. ncbi request reprint Fluorescent proteins for photoactivation experiments
    Jennifer Lippincott-Schwartz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Methods Cell Biol 85:45-61. 2008
  2. doi request reprint A palmitoylation cycle dynamically regulates partitioning of the GABA-synthesizing enzyme GAD65 between ER-Golgi and post-Golgi membranes
    Jamil Kanaani
    Department of Medicine and Diabetes Center, University of California San Francisco, HSW 1090, San Francisco, CA 94143 0534, USA
    J Cell Sci 121:437-49. 2008
  3. pmc Dynamics of putative raft-associated proteins at the cell surface
    Anne K Kenworthy
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bldg 18T, Rm 101, 18 Library Dr, Bethesda, MD 20895, USA
    J Cell Biol 165:735-46. 2004
  4. pmc ArfGAP1 dynamics and its role in COPI coat assembly on Golgi membranes of living cells
    Wei Liu
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 168:1053-63. 2005
  5. pmc The secretory membrane system in the Drosophila syncytial blastoderm embryo exists as functionally compartmentalized units around individual nuclei
    David Frescas
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 173:219-30. 2006
  6. pmc The origin and maintenance of mammalian peroxisomes involves a de novo PEX16-dependent pathway from the ER
    Peter K Kim
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 173:521-32. 2006
  7. pmc Maintenance of Golgi structure and function depends on the integrity of ER export
    T H Ward
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 155:557-70. 2001
  8. pmc Rapid cycling of lipid raft markers between the cell surface and Golgi complex
    B J Nichols
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20895, USA
    J Cell Biol 153:529-41. 2001
  9. pmc Formation of stacked ER cisternae by low affinity protein interactions
    Erik L Snapp
    Cell Biology and Metabolism Branch, National Institutes of Child Health and Human Development, National Institutes of Health, 18 Library Dr, Bldg 18T, Rm 101, Bethesda, MD 20892, USA
    J Cell Biol 163:257-69. 2003
  10. ncbi request reprint Photobleaching and photoactivation: following protein dynamics in living cells
    Jennifer Lippincott-Schwartz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Cell Biol . 2003

Collaborators

Detail Information

Publications100

  1. ncbi request reprint Fluorescent proteins for photoactivation experiments
    Jennifer Lippincott-Schwartz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Methods Cell Biol 85:45-61. 2008
    ..This chapter introduces the different types of PA-FPs and discusses their use for monitoring protein movement, protein turnover, protein interactions, and high-resolution protein localization...
  2. doi request reprint A palmitoylation cycle dynamically regulates partitioning of the GABA-synthesizing enzyme GAD65 between ER-Golgi and post-Golgi membranes
    Jamil Kanaani
    Department of Medicine and Diabetes Center, University of California San Francisco, HSW 1090, San Francisco, CA 94143 0534, USA
    J Cell Sci 121:437-49. 2008
    ..We propose that a depalmitoylation-repalmitoylation cycle serves to cycle GAD65 between Golgi and post-Golgi membranes and dynamically control levels of enzyme directed to the synapse...
  3. pmc Dynamics of putative raft-associated proteins at the cell surface
    Anne K Kenworthy
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bldg 18T, Rm 101, 18 Library Dr, Bethesda, MD 20895, USA
    J Cell Biol 165:735-46. 2004
    ..Thus, raft association is not the dominant factor in determining long-range protein mobility at the cell surface...
  4. pmc ArfGAP1 dynamics and its role in COPI coat assembly on Golgi membranes of living cells
    Wei Liu
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 168:1053-63. 2005
    ..These data suggest that ArfGAP1, coatomer and Arf1 play interdependent roles in the assembly-disassembly cycle of the COPI coat in vivo...
  5. pmc The secretory membrane system in the Drosophila syncytial blastoderm embryo exists as functionally compartmentalized units around individual nuclei
    David Frescas
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 173:219-30. 2006
    ....
  6. pmc The origin and maintenance of mammalian peroxisomes involves a de novo PEX16-dependent pathway from the ER
    Peter K Kim
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 173:521-32. 2006
    ..Finally, we demonstrate that the increase in peroxisome number in growing wild-type cells results primarily from new peroxisomes derived from the ER rather than by division of preexisting peroxisomes...
  7. pmc Maintenance of Golgi structure and function depends on the integrity of ER export
    T H Ward
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 155:557-70. 2001
    ..Instead, they suggest that the Golgi complex is a dynamic, steady-state system, whose membranes can be nucleated and are maintained by the activities of the Sar1-COPII and Arf1-coatomer systems...
  8. pmc Rapid cycling of lipid raft markers between the cell surface and Golgi complex
    B J Nichols
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20895, USA
    J Cell Biol 153:529-41. 2001
    ..The PM-Golgi cycling pathway followed by GPI-GFP could serve to regulate lipid raft distribution and function within cells...
  9. pmc Formation of stacked ER cisternae by low affinity protein interactions
    Erik L Snapp
    Cell Biology and Metabolism Branch, National Institutes of Child Health and Human Development, National Institutes of Health, 18 Library Dr, Bldg 18T, Rm 101, Bethesda, MD 20892, USA
    J Cell Biol 163:257-69. 2003
    ..This mechanism may underlie the formation of other stacked membrane structures within cells...
  10. ncbi request reprint Photobleaching and photoactivation: following protein dynamics in living cells
    Jennifer Lippincott-Schwartz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Cell Biol . 2003
    ..Widespread applications of these fluorescent-based methods are revealing new aspects of protein dynamics and the biological processes that they regulate...
  11. ncbi request reprint Insights into COPI coat assembly and function in living cells
    Jennifer Lippincott-Schwartz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Building 18T Room 101, 18 Library Drive, Bethesda, MD 20892 5430, USA
    Trends Cell Biol 16:e1-4. 2006
    ..Here, we present images and movies characterizing the dynamics of protein components of the COPI coat in living cells. We discuss the self-assembly of these coat components into a molecular machine for sorting and trafficking membranes...
  12. ncbi request reprint Dynamics of secretory membrane trafficking
    Jennifer Lippincott-Schwartz
    Section on Organelle Biology, Cell Biology and Metabolism Branch, Division of Intramural Research, NICHD, NIH, Building 18T, Room 101, 18 Library Drive, Bethesda, MD 20892 5430, USA
    Ann N Y Acad Sci 1038:115-24. 2004
    ..This has provided unexpected new insights into the organization and function of secretory and endocytic pathways within cells...
  13. ncbi request reprint Development and use of fluorescent protein markers in living cells
    Jennifer Lippincott-Schwartz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 300:87-91. 2003
    ....
  14. pmc Photoactivatable fluorescent proteins for diffraction-limited and super-resolution imaging
    Jennifer Lippincott-Schwartz
    Cell Biology and Metabolism Program, NICHD, NIH Bethesda, MD, USA
    Trends Cell Biol 19:555-65. 2009
    ..This review surveys the expanding array of PA-FPs, including their advantages and disadvantages, and highlights their use in novel imaging methodologies...
  15. ncbi request reprint Insights into secretory and endocytic membrane traffic using green fluorescent protein chimeras
    J Lippincott-Schwartz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development NICHD, National Institutes of Health NIH, Bethesda, Maryland 20892, USA
    Curr Opin Neurobiol 7:631-9. 1997
    ..In so doing, they have changed our thinking about the temporal and spatial relationships between subcellular membrane structures and the morphogenesis of secretory and endocytic organelles...
  16. ncbi request reprint Cytoskeletal proteins and Golgi dynamics
    J Lippincott-Schwartz
    Cell Biology and Metabolism Branch, NICHD, National Institute for Health, Bethesda, MD 20892 0001, USA
    Curr Opin Cell Biol 10:52-9. 1998
    ..Understanding how these associations are regulated and what roles they play in Golgi trafficking and dynamics is fundamental for insight into the spatial and functional integration of secretory membrane traffic...
  17. ncbi request reprint Building a secretory apparatus: role of ARF1/COPI in Golgi biogenesis and maintenance
    J Lippincott-Schwartz
    Cell Biology and Metabolism Branch, NICHD, NIH, Bethesda, MD 20892 5430, USA
    Histochem Cell Biol 109:449-62. 1998
    ....
  18. ncbi request reprint Unravelling Golgi membrane traffic with green fluorescent protein chimeras
    J Lippincott-Schwartz
    Cell Biology and Metabolism Branch, NICHD, NIH, Bethesda, MD 20892 5430, USA
    Trends Cell Biol 8:16-20. 1998
    ..They are also providing insights into mechanisms of protein retention and localization within Golgi membranes...
  19. ncbi request reprint Secretory protein trafficking and organelle dynamics in living cells
    J Lippincott-Schwartz
    Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Cell Dev Biol 16:557-89. 2000
    ..In so doing, they are broadening our thinking about the temporal and spatial relationships among secretory organelles and the membrane trafficking pathways that operate between them...
  20. ncbi request reprint Cell cycle maintenance and biogenesis of the Golgi complex
    J Lippincott-Schwartz
    Cell Biology and Metabolism Branch, NICHD NIH, Bethesda, MD 20892 5430, USA
    Histochem Cell Biol 114:93-103. 2000
    ....
  21. ncbi request reprint Studying protein dynamics in living cells
    J Lippincott-Schwartz
    Cell Biology and Metabolism Branch, 18 Library Drive, NICHD, NIH Bethesda, Maryland 20892 5430 USA
    Nat Rev Mol Cell Biol 2:444-56. 2001
    ..Remarkably, these powerful techniques are accessible to non-specialists using commercially available microscope systems...
  22. ncbi request reprint The secretory membrane system studied in real-time. Robert Feulgen Prize Lecture, 2001
    J Lippincott-Schwartz
    Cell Biology and Metabolism Branch, NIH NICHD, Bethesda, MD 20892 5430, USA
    Histochem Cell Biol 116:97-107. 2001
    ....
  23. pmc Lipids and cholesterol as regulators of traffic in the endomembrane system
    Jennifer Lippincott-Schwartz
    Cell Biology and Metabolism Program, NICHD, NIH, Bethesda, Maryland, USA
    Annu Rev Biophys 39:559-78. 2010
    ..We further discuss how computational modeling can allow for interpretation of experimental findings and provide mechanistic insight into these important cellular pathways...
  24. pmc Dynamics of GBF1, a Brefeldin A-sensitive Arf1 exchange factor at the Golgi
    Ting Kuang Niu
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 16:1213-22. 2005
    ..These results suggest that an Arf1-GBF1-BFA complex is formed and has a longer residence time on Golgi membranes than GBF1 or Arf1 alone...
  25. pmc Multiscale diffusion in the mitotic Drosophila melanogaster syncytial blastoderm
    Brian R Daniels
    Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 109:8588-93. 2012
    ....
  26. ncbi request reprint Four-dimensional imaging of filter-grown polarized epithelial cells
    Yoshiyuki Wakabayashi
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Histochem Cell Biol 127:463-72. 2007
    ..This new technique has wide applicability for visualization and manipulation of polarized epithelial cells...
  27. ncbi request reprint A photoactivatable GFP for selective photolabeling of proteins and cells
    George H Patterson
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 297:1873-7. 2002
    ..Here, we use the photoactivatable GFP both as a free protein to measure protein diffusion across the nuclear envelope and as a chimera with a lysosomal membrane protein to demonstrate rapid interlysosomal membrane exchange...
  28. pmc Dynamin 2 orchestrates the global actomyosin cytoskeleton for epithelial maintenance and apical constriction
    Jennifer Chua
    Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:20770-5. 2009
    ..We propose this enables Dyn2 to coordinate, in a GTPase-dependent manner, membrane recycling and actomyosin contractility during epithelial morphogenesis...
  29. pmc Localization of A20 to a lysosome-associated compartment and its role in NFkappaB signaling
    Lianyun Li
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1783:1140-9. 2008
    ..Interestingly, A20 mutants defective in membrane association also contain reduced NFkappaB inhibitory activity. These findings suggest the involvement of a lysosome-associated mechanism in A20-dependent termination of NFkappaB signaling...
  30. ncbi request reprint Dissection of COPI and Arf1 dynamics in vivo and role in Golgi membrane transport
    John F Presley
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 417:187-93. 2002
    ..This role for Arf1/coatomer might provide a model for investigating the behaviour of other coat protein systems within cells...
  31. doi request reprint Measuring protein mobility by photobleaching GFP chimeras in living cells
    Erik L Snapp
    NICHD, National Institutes of Health, Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, Bethesda, Maryland, USA
    Curr Protoc Cell Biol . 2003
    ..Together these two techniques are providing fundamentally new insights into the kinetic properties of proteins in cells...
  32. pmc Golgi inheritance in mammalian cells is mediated through endoplasmic reticulum export activities
    Nihal Altan-Bonnet
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 17:990-1005. 2006
    ..Together, these findings support a model of mitotic Golgi inheritance that involves inhibition and subsequent reactivation of cellular activities controlling the cycling of Golgi components into and out of the ER...
  33. pmc Transport through the Golgi apparatus by rapid partitioning within a two-phase membrane system
    George H Patterson
    Cell Biology and Metabolism Program, National Institutes of Health, Building 18T, Room 101, 18 Library Drive, Bethesda, MD 20892 5430, USA
    Cell 133:1055-67. 2008
    ..Simulation and experimental testing of this rapid partitioning model reproduced all the key characteristics of the Golgi apparatus, including polarized lipid and protein gradients, exponential cargo export kinetics, and cargo waves...
  34. doi request reprint Addressing membrane protein topology using the fluorescence protease protection (FPP) assay
    Holger Lorenz
    Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, Bethesda, MD, USA
    Methods Mol Biol 440:227-33. 2008
    ..To illustrate the technique, we describe its use for deciphering the topology of a membrane protein in the ER...
  35. ncbi request reprint Role of Grb2 in EGF-stimulated EGFR internalization
    Tetsuo Yamazaki
    Laboratory of Cellular and Molecular Biology, Division of Basic Science, National Cancer Institute, Bethesda, MD 20892 4255, USA
    J Cell Sci 115:1791-802. 2002
    ..These results demonstrate a role for Grb2 in events associated with a macropinocytic internalization pathway for EGFR in activated cells...
  36. pmc Coordinated elevation of mitochondrial oxidative phosphorylation and autophagy help drive hepatocyte polarization
    Dong Fu
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 110:7288-93. 2013
    ..Taken together, our results describe a metabolic shift involving a number of coordinated metabolic pathways that ultimately serve to increase energy production during cell polarization...
  37. ncbi request reprint The fluorescence protease protection (FPP) assay to determine protein localization and membrane topology
    Holger Lorenz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Building 18T Library Drive, Bethesda, Maryland 20892, USA
    Nat Protoc 1:276-9. 2006
    ..Here we provide a step-by-step protocol for the assay. As an example, we use FPP to determine which terminus of an endoplasmic reticulum (ER) transmembrane protein is lumenal and which one is facing the cytosol...
  38. pmc Membrane scission by the ESCRT-III complex
    Thomas Wollert
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, US Department of Health and Human Services, Bethesda, Maryland 20892, USA
    Nature 458:172-7. 2009
    ..The minimum set of ESCRT-III and Vps4 proteins capable of multiple cycles of vesicle detachment corresponds to the ancient set of ESCRT proteins conserved from archaea to animals...
  39. pmc DRP1-dependent mitochondrial fission initiates follicle cell differentiation during Drosophila oogenesis
    Kasturi Mitra
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 197:487-97. 2012
    ..Thus, DRP1-dependent mitochondrial fission activity is a novel regulator of the onset of follicle cell differentiation during Drosophila oogenesis...
  40. ncbi request reprint Depletion of type IA regulatory subunit (RIalpha) of protein kinase A (PKA) in mammalian cells and tissues activates mTOR and causes autophagic deficiency
    Manos Mavrakis
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Hum Mol Genet 15:2962-71. 2006
    ..These data suggest that RIalpha deficiency decreases autophagy by the activation of mTOR, providing a molecular basis to autophagic deficiency in PPNAD...
  41. doi request reprint Using photoactivatable proteins to monitor autophagosome lifetime
    Dale W Hailey
    Section on Organelle Biology, Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, Bethesda, Maryland, USA
    Methods Enzymol 452:25-45. 2009
    ..This assay is applicable to both engineered tissue culture models and transgenic organisms expressing photoactivatable proteins fused to Atg8 homologs...
  42. pmc Ubiquitin signals autophagic degradation of cytosolic proteins and peroxisomes
    Peter Kijun Kim
    Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 105:20567-74. 2008
    ..Mammalian cells thus use a common pathway involving ubiquitin and p62 for targeting diverse types of substrates for autophagy...
  43. pmc A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells
    Dylan T Burnette
    National Institute of Child Health and Human Development and 2 National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892
    J Cell Biol 205:83-96. 2014
    ....
  44. pmc Plasma membrane polarity and compartmentalization are established before cellularization in the fly embryo
    Manos Mavrakis
    Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 16:93-104. 2009
    ..We propose that epithelial-like properties and an intact F-actin network compartmentalize the PM and shape morphogen gradients in the syncytial embryo...
  45. ncbi request reprint Molecular basis for Golgi maintenance and biogenesis
    Nihal Altan-Bonnet
    Cell Biology and Metabolism Branch, National Institutes of Child Health and Development, National Institutes of Health, Bethesda, Maryland, MD 20892, USA
    Curr Opin Cell Biol 16:364-72. 2004
    ..Information gained on how different proteins participate in this process can provide important insights for understanding the Golgi's global functions within cells...
  46. pmc Functional nanoscale organization of signaling molecules downstream of the T cell antigen receptor
    Eilon Sherman
    Laboratory of Cellular and Molecular Biology, CCR, NCI, NIH, Bethesda, MD 20892, USA
    Immunity 35:705-20. 2011
    ..These results extend our understanding of the mechanism of T cell activation and the formation and organization of TCR-mediated signaling complexes, findings also relevant to other receptor systems...
  47. pmc Bleaching/blinking assisted localization microscopy for superresolution imaging using standard fluorescent molecules
    Dylan T Burnette
    the Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 108:21081-6. 2011
    ..We also show that BaLM works with a spectrum of fluorescent molecules in the same sample. Thus, BaLM extends single molecule-based superresolution localization to samples labeled with multiple conventional fluorescent probes...
  48. pmc Mitochondria supply membranes for autophagosome biogenesis during starvation
    Dale W Hailey
    The Eunice Kennedy Shriver National Institutes of Child Health and Human Development, 18 Library Drive, Bethesda, MD 20892, USA
    Cell 141:656-67. 2010
    ..Disruption of mitochondria/ER connections by mitofusin2 depletion dramatically impairs starvation-induced autophagy. Mitochondria thus play a central role in starvation-induced autophagy, contributing membrane to autophagosomes...
  49. pmc Determining membrane protein topologies in single cells and high-throughput screening applications
    Christian Wunder
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
    Curr Protoc Cell Biol . 2010
    ..First, we describe the FPP assay using fluorescence microscopy for single adherent cells, and second, we outline the FPP assay for high-throughput screening applications...
  50. pmc Analysis of mitochondrial dynamics and functions using imaging approaches
    Kasturi Mitra
    National Institute of Child Health and Human Development, NIH, Bethesda, Maryland, USA
    Curr Protoc Cell Biol . 2010
    ..A protocol for measuring mitochondrial potential has also been included in this unit. In conclusion, the protocols described here will aid the understanding of mitochondrial structure-function relationship...
  51. pmc The fusome mediates intercellular endoplasmic reticulum connectivity in Drosophila ovarian cysts
    Erik L Snapp
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 15:4512-21. 2004
    ....
  52. pmc Tubular network formation protects mitochondria from autophagosomal degradation during nutrient starvation
    Angelika S Rambold
    the Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 108:10190-5. 2011
    ....
  53. pmc Rab11a and myosin Vb are required for bile canalicular formation in WIF-B9 cells
    Yoshiyuki Wakabayashi
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:15087-92. 2005
    ..In this model, polarization is initiated upon delivery of rab11a-myosin Vb-containing membranes to the surface, which causes plasma membrane at the site of delivery to differentiate into apical domain (bile canaliculus)...
  54. pmc A role for Arf1 in mitotic Golgi disassembly, chromosome segregation, and cytokinesis
    Nihal Altan-Bonnet
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 100:13314-9. 2003
    ....
  55. ncbi request reprint Delivery of raft-associated, GPI-anchored proteins to the apical surface of polarized MDCK cells by a transcytotic pathway
    Roman Polishchuk
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Nat Cell Biol 6:297-307. 2004
    ..Thus, apical sorting of these GPI-anchored proteins occurs at the plasma membrane, rather than at the TGN...
  56. pmc Computational model of cytokinetic abscission driven by ESCRT-III polymerization and remodeling
    Natalie Elia
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Development, National Institutes of Health, Bethesda, MD, USA
    Biophys J 102:2309-20. 2012
    ..We substantiate the model by theoretical analysis of the membrane elastic energy and by experimental verification of the major model assumptions...
  57. pmc Quantitative analysis of photoactivated localization microscopy (PALM) datasets using pair-correlation analysis
    Prabuddha Sengupta
    the Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MA, USA
    Bioessays 34:396-405. 2012
    ..Using rigorous statistical algorithms to estimate the number of detected proteins, this approach allows the spatial organization of molecules to be quantitatively described...
  58. pmc Bone marrow homing and engraftment of human hematopoietic stem and progenitor cells is mediated by a polarized membrane domain
    Andre Larochelle
    Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Blood 119:1848-55. 2012
    ....
  59. pmc Bile acid stimulates hepatocyte polarization through a cAMP-Epac-MEK-LKB1-AMPK pathway
    Dong Fu
    Cell Biology and Metabolism Program, the Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 108:1403-8. 2011
    ..Linkage between bile acid synthesis, hepatocyte polarization, and regulation of energy metabolism is likely important in normal hepatocyte development and disease...
  60. pmc Structural basis for midbody targeting of spastin by the ESCRT-III protein CHMP1B
    Dong Yang
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, US Department of Health and Human Services, Bethesda, Maryland 20892, USA
    Nat Struct Mol Biol 15:1278-86. 2008
    ..Point mutants in the CHMP1B binding site of spastin block recruitment of spastin to the midbody and impair cytokinesis...
  61. pmc The organization of engaged and quiescent translocons in the endoplasmic reticulum of mammalian cells
    Erik L Snapp
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, 18 Library Dr, Bldg 18, Rm 101, Bethesda, MD 20892, USA
    J Cell Biol 164:997-1007. 2004
    ....
  62. pmc Culturing MDCK cells in three dimensions for analyzing intracellular dynamics
    Natalie Elia
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
    Curr Protoc Cell Biol . 2009
    ..This unit discusses technical aspects for culturing and imaging MDCK 3-D culture for both fixed 3-D cultures and live-cell imaging...
  63. pmc A role for actin arcs in the leading-edge advance of migrating cells
    Dylan T Burnette
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Cell Biol 13:371-81. 2011
    ..The actin arc thereby serves as a structural element underlying the temporal and spatial connection between the lamellipodium and the lamella during directed cell motion...
  64. pmc Monitoring chaperone engagement of substrates in the endoplasmic reticulum of live cells
    Erik L Snapp
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, 18 Library Drive, Building 18, Room 101, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:6536-41. 2006
    ..Our findings provide insight into the normal organization and dynamics of an ER chaperone and characterize the capacity of the ER to maintain homeostasis during acute changes in chaperone activity and availability...
  65. pmc Dynamics of endosomal sorting complex required for transport (ESCRT) machinery during cytokinesis and its role in abscission
    Natalie Elia
    Cell Biology and Metabolism Program, The Eunice Kennedy Shriver National Institute of Child Health and Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 108:4846-51. 2011
    ..That arrival of ESCRT-III and VPS4 correlates both spatially and temporally with the abscission event suggests a direct role for these proteins in cytokinetic membrane abscission...
  66. pmc Quantifying spatial organization in point-localization superresolution images using pair correlation analysis
    Prabuddha Sengupta
    the Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
    Nat Protoc 8:345-54. 2013
    ..Researchers need to have substantial experience in single-molecule imaging and statistical analysis to conduct the experiments and carry out this analysis...
  67. pmc The open reading frame 3a protein of severe acute respiratory syndrome-associated coronavirus promotes membrane rearrangement and cell death
    Eric C Freundt
    Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 84:1097-109. 2010
    ..These results establish an important role for ORF 3a in SARS-CoV-induced cell death, Golgi fragmentation, and the accumulation of intracellular vesicles...
  68. ncbi request reprint Coat proteins: shaping membrane transport
    Juan S Bonifacino
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Building 18T Room 101, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Rev Mol Cell Biol 4:409-14. 2003
    ....
  69. pmc Regulation of bile canalicular network formation and maintenance by AMP-activated protein kinase and LKB1
    Dong Fu
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institute of Health, Bethesda, MD 20892, USA
    J Cell Sci 123:3294-302. 2010
    ..These studies reveal that AMPK and its upstream kinase, LKB1, regulate canalicular network formation and maintenance...
  70. ncbi request reprint Fluorescence protease protection of GFP chimeras to reveal protein topology and subcellular localization
    Holger Lorenz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bldg 18T Library Drive, Bethesda, Maryland 20892, USA
    Nat Methods 3:205-10. 2006
    ..The success of the FPP assay in characterizing the topology of the tested proteins within their appropriate compartments suggests this technique has wide applicability in studying protein topology and localization within the cell...
  71. doi request reprint High-density mapping of single-molecule trajectories with photoactivated localization microscopy
    Suliana Manley
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Methods 5:155-7. 2008
    ..By probing distinct subsets of molecules, sptPALM can provide insight into the origins of spatial and temporal heterogeneities in membranes...
  72. pmc Hijacking components of the cellular secretory pathway for replication of poliovirus RNA
    George A Belov
    National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 8011, USA
    J Virol 81:558-67. 2007
    ..The recruitment of Arf to membranes via specific GEFs by poliovirus proteins provides an important clue toward identifying cellular pathways utilized by the virus to form its membranous replication complex...
  73. pmc An evolving paradigm for the secretory pathway?
    Jennifer Lippincott-Schwartz
    Cell Biology and Metabolism Program, Eunice Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 22:3929-32. 2011
    ..As a result, we may be seeing an example of how a paradigm is evolving to account for the results of new technologies and their new ways of describing cellular processes...
  74. ncbi request reprint Selective photolabeling of proteins using photoactivatable GFP
    George H Patterson
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Methods 32:445-50. 2004
    ..Here, we discuss the technical aspects for using one of these recently developed photoactivatable fluorescent proteins, PA-GFP...
  75. pmc Midbody targeting of the ESCRT machinery by a noncanonical coiled coil in CEP55
    Hyung Ho Lee
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892, USA
    Science 322:576-80. 2008
    ..Both ALIX and ESCRT-I are required for cytokinesis, which suggests that multiple CEP55 dimers are required for function...
  76. ncbi request reprint Retention of pendrin in the endoplasmic reticulum is a major mechanism for Pendred syndrome
    Pnina Rotman-Pikielny
    Clinical Endocrinology Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    Hum Mol Genet 11:2625-33. 2002
    ..These findings suggest that ER retention and defective plasma membrane targeting of pendrin mutants play a key role in the pathogenesis of Pendred syndrome...
  77. pmc Bridging structure and process in developmental biology through new imaging technologies
    Jennifer Lippincott-Schwartz
    Eunice Kennedy Shriver Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 21:5-10. 2011
    ..This essay surveys the recent advances in imaging, highlighting emerging capabilities with an eye toward those poised to have the greatest impact on developmental biology...
  78. pmc Emerging in vivo analyses of cell function using fluorescence imaging (*)
    Jennifer Lippincott-Schwartz
    The Eunice Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 5034, USA
    Annu Rev Biochem 80:327-32. 2011
    ..They provide researchers with exciting new tools to explore how cellular processes are organized and their activity regulated in vivo...
  79. ncbi request reprint mTOR kinase and the regulatory subunit of protein kinase A (PRKAR1A) spatially and functionally interact during autophagosome maturation
    Manos Mavrakis
    Cell Biology and Metabolism Branch, Section on Endocrinology and Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 5430, USA
    Autophagy 3:151-3. 2007
    ..Because RIalpha regulates the phosphorylation and activity of mTOR kinase, which we now show localizes on autophagosomal membranes, the possibility emerges that the RIalpha-mTOR complex acts at the level of autophagosome maturation...
  80. pmc Superresolution imaging using single-molecule localization
    George Patterson
    Biophotonics Section, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Phys Chem 61:345-67. 2010
    ..Single-molecule-based superresolution imaging thus offers exciting possibilities for obtaining molecular-scale information on biological events occurring at variable timescales...
  81. pmc Intercellular transfer to signalling endosomes regulates an ex vivo bone marrow niche
    Jennifer M Gillette
    Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, Maryland 20892, USA
    Nat Cell Biol 11:303-11. 2009
    ..These findings identify a mechanism involving intercellular transfer to signalling endosomes for targeted regulation of signalling and remodelling events within an ex vivo osteoblastic niche...
  82. pmc Depalmitoylated Ras traffics to and from the Golgi complex via a nonvesicular pathway
    J Shawn Goodwin
    Department of Molecular Physiology and Biophysics and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    J Cell Biol 170:261-72. 2005
    ..We propose that a cycle of depalmitoylation and repalmitoylation regulates the time course and sites of Ras signaling by allowing the protein to be released from the cell surface and rapidly redistributed to intracellular membranes...
  83. ncbi request reprint The uses of green fluorescent protein in mammalian cells
    Theresa H Ward
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
    Methods Biochem Anal 47:305-37. 2006
  84. ncbi request reprint Membrane trafficking: coat control by curvature
    Jennifer Lippincott-Schwartz
    Nature 426:507-8. 2003
  85. pmc Rab9 GTPase is required for replication of human immunodeficiency virus type 1, filoviruses, and measles virus
    James L Murray
    National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Virol 79:11742-51. 2005
    ..These results suggest that Rab9 is an important cellular target for inhibiting diverse viruses and help to define a late-endosome-to-plasma-membrane vesicular transport pathway important in viral assembly...
  86. ncbi request reprint Fluorescence imaging techniques for studying Drosophila embryo development
    Manos Mavrakis
    Institute of Developmental Biology of Marseille Luminy, UMR6216 CNRS Université de la Méditerranée, Marseille, France
    Curr Protoc Cell Biol . 2008
    ....
  87. pmc Nup98 is a mobile nucleoporin with transcription-dependent dynamics
    Eric R Griffis
    Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Mol Biol Cell 13:1282-97. 2002
    ..These data give rise to a model in which Nup98 aids in direction of RNAs to the nuclear pore and provide the first potential mechanism for the role of a mobile nucleoporin...
  88. pmc Stable binding of ATF6 to BiP in the endoplasmic reticulum stress response
    Jingshi Shen
    Department of Biological Sciences, Columbia University, Fairchild 813B, MC 2420, 1212 Amsterdam Avenue, New York, NY 10027, USA
    Mol Cell Biol 25:921-32. 2005
    ..We propose that stable BiP binding is essential for ATF6 regulation and that ER stress dissociates BiP from ATF6 by actively restarting the BiP ATPase cycle...
  89. pmc Endoplasmic reticulum export sites and Golgi bodies behave as single mobile secretory units in plant cells
    Luis L P daSilva
    Centre of Plant Sciences, University of Leeds, LS2 9JT, United Kingdom
    Plant Cell 16:1753-71. 2004
    ..Moreover, we could demonstrate using the drug brefeldin A that formation of ERES is strictly dependent on a functional retrograde transport route from the Golgi apparatus...
  90. ncbi request reprint Electron tomography of the Maurer's cleft organelles of Plasmodium falciparum-infected erythrocytes reveals novel structural features
    Eric Hanssen
    Department of Biochemistry and Centre of Excellence for Coherent X ray Science, La Troube University, Melbourne, Victoria 3086, Australia
    Mol Microbiol 67:703-18. 2008
    ..Spot fluorescence photobleaching was used to demonstrate the absence of a lipid continuum between the Maurer's clefts and parasite membranes and the host plasma membrane...
  91. pmc Intracellular trafficking of bile salt export pump (ABCB11) in polarized hepatic cells: constitutive cycling between the canalicular membrane and rab11-positive endosomes
    Yoshiyuki Wakabayashi
    Department of Physiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Mol Biol Cell 15:3485-96. 2004
    ..The pathway between canalicular and intracellular membranes that BSEP constitutively cycles within could serve to regulate apical pools of BSEP as well as other apical membrane transporters...
  92. ncbi request reprint Nucleocytoplasmic shuttling mediates the dynamic maintenance of nuclear Dorsal levels during Drosophila embryogenesis
    Robert DeLotto
    Department of Molecular Biology, University of Copenhagen, Ole Maaløes Vej 5, DK 2200 Copenhagen N, Denmark
    Development 134:4233-41. 2007
    ..A model is proposed in which the generation and maintenance of the Dorsal gradient is a consequence of an active process involving both restricted long-range diffusion and the balancing of nuclear import with nuclear export...
  93. pmc Membrane protein transport between the endoplasmic reticulum and the Golgi in tobacco leaves is energy dependent but cytoskeleton independent: evidence from selective photobleaching
    Federica Brandizzi
    Research School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane, Oxford OX3 0BP, United Kingdom
    Plant Cell 14:1293-309. 2002
    ..Our results indicate that in plant cells, the Golgi apparatus is a dynamic membrane system whose components continuously traffic via membrane trafficking pathways regulated by brefeldin A- and N-ethylmaleimide-sensitive machinery...
  94. pmc Active translocon complexes labeled with GFP-Dad1 diffuse slowly as large polysome arrays in the endoplasmic reticulum
    Andrei V Nikonov
    Department of Cell Biology, New York University School of Medicine, New York 10016, USA
    J Cell Biol 158:497-506. 2002
    ..Our findings that TCs assembled into membrane-bound polysomes diffuse slowly within the ER have mechanistic implications for the segregation of the ER into smooth and rough domains...
  95. ncbi request reprint Starvation and ULK1-dependent cycling of mammalian Atg9 between the TGN and endosomes
    Andrew R J Young
    Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    J Cell Sci 119:3888-900. 2006
    ..We speculate that starvation-induced autophagy, which requires mAtg9, may rely on an alteration of the steady-state trafficking of mAtg9, in a Atg1-dependent manner...
  96. ncbi request reprint Imaging intracellular fluorescent proteins at nanometer resolution
    Eric Betzig
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA
    Science 313:1642-5. 2006
    ....
  97. ncbi request reprint The nuclear import of protein kinase D3 requires its catalytic activity
    Osvaldo Rey
    Division of Digestive Diseases, Department of Medicine, CURE Digestive Diseases Research Center and Molecular Biology Institute, David Geffen School of Medicine, University of California, Los Angeles, California 90095 1786, USA
    J Biol Chem 281:5149-57. 2006
    ....
  98. ncbi request reprint Illuminating COPII coat dynamics
    Wei Liu
    Nat Struct Mol Biol 12:106-7. 2005
  99. ncbi request reprint Anything can work
    Jennifer Lippincott-Schwartz
    Biotechniques 40:419. 2006
  100. ncbi request reprint Unifying nomenclature for the isoforms of the lysosomal membrane protein LAMP-2
    Eeva Liisa Eskelinen
    University of Kiel, Institute of Biochemistry, Kiel, Germany
    Traffic 6:1058-61. 2005
    ..To avoid confusion in the literature, we further propose to adopt the use of capital letters for the updated nomenclature of all the isoforms in all three species: LAMP-2A, LAMP-2B, and LAMP-2C...