Research Topics
Genomes and GenesSpecies | Lei LiSummaryAffiliation: National Institutes of Health Country: USA Publications
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Publications
A subcortical maternal complex essential for preimplantation mouse embryogenesisLei Li
Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
Dev Cell 15:416-25. 2008..However, these embryos do not progress beyond cleavage stage development and female mice are sterile. The proteins are conserved in humans, and similar maternal effect mutations may result in recurrent embryonic loss...
Maternally derived FILIA-MATER complex localizes asymmetrically in cleavage-stage mouse embryosMami Ohsugi
Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
Development 135:259-69. 2008..If so, the plasticity of the FILIA-MATER complex localization may reflect the regulative nature of preimplantation mouse development...- Maternal control of early mouse developmentLei Li
Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
Development 137:859-70. 2010..Nevertheless, significant gaps remain in our mechanistic understanding of the networks that regulate early mammalian embryogenesis, which provide an impetus and opportunities for future investigations... - A histone-fold complex and FANCM form a conserved DNA-remodeling complex to maintain genome stabilityZhijiang Yan
Laboratory of Genetics, National Institute of Aging, National Institutes of Health, Baltimore, MD 21224, USA
Mol Cell 37:865-78. 2010..Thus, FANCM-MHF is an essential DNA-remodeling complex that protects replication forks from yeast to human... - BLAP75/RMI1 promotes the BLM-dependent dissolution of homologous recombination intermediatesLeonard Wu
Cancer Research UK, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
Proc Natl Acad Sci U S A 103:4068-73. 2006..Implications of the conserved ability of type IA topoisomerases to catalyze dissolution and how the evolution of factors such as BLAP75/RMI1 might confer specificity on the execution of this process are discussed... - Verification and tissue-specific expression of nifU-like gene from the amphioxus Branchiostoma belcheriLei Li
Department of Marine Biology, Ocean University of China, Qingdao, People s Republic of China
DNA Seq 18:363-70. 2007..Taken together, it is highly likely that NifU-like protein plays some tissue-dependent and critical role in amphioxus... - Development of a high throughput transformation system for insertional mutagenesis in Magnaporthe oryzaeMelania F Betts
Department of Plant Sciences, Division of Plant Pathology and Microbiology, University of Arizona, Tucson, AZ 85721 0036, USA
Fungal Genet Biol 44:1035-49. 2007..3% of conidia being transformed. The phenotypic data is accessible via a public database called MGOS and all strains are publicly available. This represents the most comprehensive insertional mutagenesis analysis of a fungal pathogen... - hMRE11 plays an important role in U937 cellular response to DNA double-strand breaks following etoposideLing Bo Liu
Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
Zhongguo Shi Yan Xue Ye Xue Za Zhi 15:10-5. 2007..95 +/- 2.91% (P < 0.05). It is concluded that the nuclear focus formation of hMRE11 protein may be a response to DNA damage induced by topoisomerase II inhibitor VP-16 in human promonocytic cell line U937... - Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screeningChenggang Wu
Department of Biochemistry and the Siebens Drake Research Institute, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada
Proteomics 7:1775-85. 2007.... - Mirl is highly upregulated and localized to nuclei during infectious hyphal growth in the rice blast fungusLei Li
Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, USA
Mol Plant Microbe Interact 20:448-58. 2007..These data suggest that the expression but not the nuclear localization of MIR1 is specific to infectious hyphae and that reporter genes based on MIR1 may be suitable for monitoring infectious growth in M. grisea... - Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signalsJae-Il Park
Department of Biochemistry and Molecular Biology and Program in Genes and Development, University of Texas M.D. Anderson Cancer Center, Houston, Texas 77030, USA
Dev Cell 11:683-95. 2006.... - Rad17 phosphorylation is required for claspin recruitment and Chk1 activation in response to replication stressXin Wang
Department of Experimental Radiation Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
Mol Cell 23:331-41. 2006..These findings reveal a phosphorylation-dependent function of Rad17 in an ATR-Rad17-Claspin-Chk1-signaling cascade that responds to specific replication stress... - [Blocking the escape of leukemic cells from killing of T cell by combining anti-Fas ribozyme and CD80-IgG fusion protein]Ling-Bo Liu
Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
Zhonghua Yi Xue Za Zhi 85:3469-74. 2005.... - Construction of a novel system for cell surface display of heterologous proteins on Pichia pastorisQingjie Wang
State Key Laboratory of Microbial Technology, Life Science School, Shandong University, Jinan, 250100, P R China
Biotechnol Lett 29:1561-6. 2007..pastoris surface was confirmed by confocal laser scanning microscopy. Fluorescence microscopy and western blot analysis confirmed that EGFP can be successfully cleaved from the cell surface by treating with enterokinase... - Characterization, evolution and tissue-specific expression of AmphiCalbin, a novel gene encoding EF-hand calcium-binding protein in amphioxus Branchiostoma belcheriJing Luan
Department of Marine Biology, Ocean University of China, Qingdao 266003, China
Acta Biochim Biophys Sin (Shanghai) 39:891-900. 2007..It is proposed that AmphiCalbin might play a role in the digestive system and gonads. These observations lay the foundation for further understanding of the function of the unnamed EFCaBPs... 
Linear motif atlas for phosphorylation-dependent signalingMartin Lee Miller
Center for Biological Sequence Analysis, Technical University of Denmark, 2800 Lyngby, Denmark
Sci Signal 1:ra2. 2008..The atlas is available as a community resource (http://netphorest.info)...- Fibrinogen-related protein from amphioxus Branchiostoma belcheri is a multivalent pattern recognition receptor with a bacteriolytic activityChunxin Fan
Department of Marine Biology, Ocean University of China, Qingdao 266003, China
Mol Immunol 45:3338-46. 2008..It also bolsters the notion that the hepatic caecum of amphioxus is equivalent to the vertebrate liver, acting as a major tissue in acute phase response... - The SH3 domain--a family of versatile peptide- and protein-recognition moduleTomonori Kaneko
Department of Biochemistry and the Siebens Drake Medical Research Institute, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario N6A 5C1, Canada
Front Biosci 13:4938-52. 2008.... - Optimization of an effective directed differentiation medium for differentiating mouse bone marrow mesenchymal stem cells into hepatocytes in vitroXiao Lei Shi
Department of Hepatobiliary Surgery, Affiliated Drum Tower Hospital, Medical College of Nanjing University, No 321 Zhongshan Road, Nanjing 210008, PR China
Cell Biol Int 32:959-65. 2008..Uniform design was adequate for choosing the MEDDM of mMSCs. MEDDM containing 35 ng/ml FGF and 30 ng/ml OSM was effective in differentiating mMSCs into hepatocytes... - Modulation of brassinosteroid-regulated gene expression by Jumonji domain-containing proteins ELF6 and REF6 in ArabidopsisXiaofei Yu
Department of Genetics, Development and Cell Biology, Plant Science Institute, Iowa State University, Ames, IA 50011, USA
Proc Natl Acad Sci U S A 105:7618-23. 2008..Thus, our study establishes an important mechanism by which Jmj domain proteins modulate specific gene expression by interacting with pathway-specific transcription factors such as BES1... - Coexpression of adrenomedullin and its receptors in the reproductive system of the rat: effects on steroid secretion in rat ovaryYuk Yin Li
Department of Physiology, Faculty of Medicine, The University of Hong Kong, Hong Kong, China
Biol Reprod 79:200-8. 2008..The presence of ADM and the gene expression of ADM and its receptor components in the female reproductive system suggest a paracrine effect of ADM on ovarian steroidogenesis... - Construction of a novel Pichia pastoris cell-surface display system based on the cell wall protein Pir1Qingjie Wang
Life Science School, Shandong University, Jinan, 250100, People s Republic of China
Curr Microbiol 56:352-7. 2008..The repetitive sequences of Pir1 are required for cell wall retention, and the C-terminal sequence contributes to the irregular distribution of fusion proteins in P. pastoris... - Effects of dexamethasone and dibutyryl cAMP on stanniocalcin-1 mRNA expression in rat primary Sertoli and Leydig cellsLei Li
Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong
Mol Cell Endocrinol 283:96-103. 2008..Taken together, this study provides the first evidence of the regulation of STC1 expression in the male reproductive system... - Picomolar inhibitors as transition-state probes of 5'-methylthioadenosine nucleosidasesJemy A Gutierrez
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, 10461, USA
ACS Chem Biol 2:725-34. 2007..Comparing K d ratios for mimics of early and late transition states removes limitations inherent to the enzyme and provides a better predictive tool in discriminating between possible transition-state structures... - Experimental study of recombinant eukaryotic expression vector of human eNOS in ECV304Tong Qiao
Department of Vascular Surgery, Affiliated Drum Tower Hospital of Medical school, Nanjing University, China
Swiss Med Wkly 136:19-25. 2006..We designed the eukaryotic expression vector pcDNA3.1-eNOS to study the regulated expression of eNOS (in the presence of various chemical agents) and to evaluate the exogenous NO effect on EC proliferation in vitro... - Prokaryotic expression and polyclonal antibody preparation of novel ZLG10 protein involved in infection of RSV on SPC-A1 cellsLei Li
Institute of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, PR China
Protein Expr Purif 41:170-6. 2005..Together, our data demonstrate that ZLG10, a novel protein expressed and purified in this report, might be a potential effective therapeutic candidate for treating RSV infections... - Advances in the study of luminescence probes for proteinsChangxia Sun
Key Laboratory for Colloid and Interface Chemistry of Education Ministry, Department of Chemistry, Shandong University, Jinan 250100, PR China
J Chromatogr B Analyt Technol Biomed Life Sci 803:173-90. 2004..Preliminary results indicate that they possess great potential for ultrasensitive protein detection. This review summarizes recent developments of the above-mentioned probes for proteins and 195 references are cited... - Determination of dysprosium by resonance light scattering technique in the presence of BPMPHDShuna Sun
Key Lab for Colloid and Interface Chemistry of Education Ministry, Department of Chemistry, Shandong University, Jinan 250100, China
Spectrochim Acta A Mol Biomol Spectrosc 60:261-4. 2004..0 x 10(-10)-1.0 x 10(-5) mol l(-1). The detection limit (S/N=2) is 5.6 x 10(-12) mol l(-1). Synthetic samples are determined satisfactorily. A new sensitive method for detection of dysprosium has been proposed... - Gene fusion of molecular adjuvant C3d to hCGbeta enhances the anti-hCGbeta antibody response in DNA immunizationDa Jin Li
Institute of Obstetrics and Gynecology, Fudan University, 200011 Shanghai, People s Republic of China
J Reprod Immunol 60:129-41. 2003..To express the hCGbeta-C3d3 fusion protein in a CHO cell continual expression system to investigate further the adjuvant effects of C3d on contraceptive vaccination... - The COP9 signalosome: an alternative lid for the 26S proteasome?Lei Li
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520 8104, USA
Trends Cell Biol 13:507-9. 2003..g. deubiquitination). Here, we discuss how these findings support a central role for CSN in ubiquitination of substrate proteins and their proteolysis.. - Synleurin, a novel leucine-rich repeat protein that increases the intensity of pleiotropic cytokine responsesWei Wang
Human Genome Sciences, Inc, 9410 Key West Avenue, Rockville, MD 20850, USA
Biochem Biophys Res Commun 305:981-8. 2003..Synleurin gene (slrn) maps to human chromosome at 5q12. The name synleurin reflects its synergistic effect on cytokine stimulation and its prominent leucine-rich repeats... - [Analysis of protein phosphorylation by combination of IMAC, phosphatase with biological mass spectrometry]Jing-lan Wang
Beijing Institute of Radiation Medicine, Beijing 100850, China
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 35:459-66. 2003..Finally, the phosphorylation sites were determined by tandem mass spectrometry analysis and database searching... - Identification and functional characterization of a novel R621C mutation in the synphilin-1 gene in Parkinson's diseaseFrank P Marx
Department of Neurology, Laboratory of Neurodegeneration, University of Tubingen, Tubingen, Germany
Hum Mol Genet 12:1223-31. 2003.... - Isolation and characterization of a novel human NM23-H1B gene, a different transcript of NM23-H1Xiaohua Ni
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, P R China
J Hum Genet 48:96-100. 2003..A high transcription level was also found in grade IV prostatic adenocarcinoma PC3... - Src kinase mediates phosphatidylinositol 3-kinase/Akt-dependent rapid endothelial nitric-oxide synthase activation by estrogenM Page Haynes
Section of Cardiovascular Medicine, Department of Pharmacology, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA
J Biol Chem 278:2118-23. 2003..This complex formation results in the successive activation of PI3-kinase, Akt, and eNOS with consequent enhanced NO release, implicating c-Src as a critical upstream regulator of the estrogen-stimulated PI3-kinase/Akt/eNOS pathway... - XPD/ERCC2 EXON 8 Polymorphisms: rarity and lack of significance in risk of squamous cell carcinoma of the head and neckErich M Sturgis
Department of Head and Neck Surgery, Box 441, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston 77030 4009, USA
Oral Oncol 38:475-7. 2002..The objective was to assess whether two polymorphisms in the nucleotide excision repair gene XPD (ERCC2) are markers of SCCHN risk... - Dual neuroprotective signaling mediated by downregulating two distinct phosphatase activities of PTENKe Ning
Division of Cellular and Molecular Biology, Toronto Western Research Institute, University Health Network, Toronto, Canada M5T 2S8
J Neurosci 24:4052-60. 2004.... - Two PAK kinase genes, CHM1 and MST20, have distinct functions in Magnaporthe griseaLei Li
Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, USA
Mol Plant Microbe Interact 17:547-56. 2004..However, CHM1 appears to be essential for appressorial penetration and CHM1 and MST20 may have redundant functions in M. grisea... - Disruption of PTEN coupling with 5-HT2C receptors suppresses behavioral responses induced by drugs of abuseShao Ping Ji
Neuropsychiatry Research Unit, Department of Psychiatry, University of Saskatchewan, 103 Wiggins Road, Saskatoon, Saskatchewan, Canada S7N 5E4
Nat Med 12:324-9. 2006..These results suggest a potential strategy for treating drug addiction with the Tat-3L4F peptide... - Dynamic nature of cleavage bodies and their spatial relationship to DDX1 bodies, Cajal bodies, and gemsLei Li
Department of Oncology, University of Alberta, Cross Cancer Institute, Edmonton, Alberta T6G 1Z2, Canada
Mol Biol Cell 17:1126-40. 2006..Our results suggest that cleavage body components are highly dynamic in nature... - Protein-protein coupling/uncoupling enables dopamine D2 receptor regulation of AMPA receptor-mediated excitotoxicityShengwei Zou
Department of Neuroscience, Centre for Addiction and Mental Health, University of Toronto, Toronto, Ontario, Canada M5T 1R8
J Neurosci 25:4385-95. 2005..These data may provide a new avenue to identify specific targets for therapeutics to modulate glutamate receptor-governed diseases, such as stroke... - Transfection of promyelocytic leukemia in retrovirus vector inhibits growth of human bladder cancer cellsLei Li
Institute of Urology, First Hospital of Xi'an Jiaotong University, China
Acta Pharmacol Sin 26:610-5. 2005.... - Sensitivity to phosphonoacetic acid: a new phenotype to probe DNA polymerase delta in Saccharomyces cerevisiaeLei Li
Department of Biological Sciences, University of Alberta, Edmonton, Canada
Genetics 170:569-80. 2005..We propose that pol3-L612M cells need the Rad27 flap endonuclease and MMR complexes composed of Msh2/Msh6, Mlh1/Pms1, and Exo1 for correct processing of Okazaki fragments... - BLAP75, an essential component of Bloom's syndrome protein complexes that maintain genome integrityJinhu Yin
Laboratory of Genetics, National Institute on Aging, National Institute of Health, Baltimore, MD 21224, USA
EMBO J 24:1465-76. 2005..Thus, BLAP75 is an essential component of the BLM-associated cellular machinery that maintains genome integrity... - Generation of a dataset for studying ligand effect on homodimer interfaceLei Li
MAE, NTU, Singapore
Front Biosci 10:1977-84. 2005..We also found that ligands occupying = 7% interface area have negligible effect on dimer interaction... - High level expression, purification, and characterization of the shrimp antimicrobial peptide, Ch-penaeidin, in Pichia pastorisLei Li
School of Life Sciences, Shandong University, Jinan 250100, China
Protein Expr Purif 39:144-51. 2005..Together, these observations indicate that rCHP is an effective antimicrobial peptide that can be successfully produced at high levels in the yeast, and therefore may be a potential antimicrobial candidate for practical use... - Cellular localization and role of kinase activity of PMK1 in Magnaporthe griseaKenneth S Bruno
Department of Botany and Plant Pathology, Lilly Hall, Purdue University, West Lafayette, IN 47907, USA
Eukaryot Cell 3:1525-32. 2004..Overexpression of these PMK1 mutant alleles may interfere with the function of native PMK1 during appressorium formation... - [Mutations in beta myosin heavy chain gene: two mutations in Chinese with familial hypertrophic cardiomyopathy and the correlation between the genotype and phenotype]Wen-li Xie
Department of Cardiology, Second Hospital, Peking University, Beijing 100044, China
Zhonghua Yi Xue Za Zhi 84:1610-3. 2004..Two mutations have different phenotypes. In one family, the identical mutation has different phenotypes and prognoses. The heterogeneity of phenotype suggests that multiple factors be involved in the pathogenesis... - Human XPA and RPA DNA repair proteins participate in specific recognition of triplex-induced helical distortionsKaren M Vasquez
Department of Carcinogenesis, University of Texas M D Anderson Cancer Center, Science Park Research Division, Park Road 1 C, Smithville, TX 78957, USA
Proc Natl Acad Sci U S A 99:5848-53. 2002..Addition of XPA decreases the nonspecific DNA aggregate formation. These results support the hypothesis that the NER machinery is targeted to helical distortions and demonstrate that RPA can recognize damaged DNA even without XPA...