Research Topics
Species | Andre LarochelleSummaryAffiliation: National Institutes of Health Country: USA Publications
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Detail Information
Publications
Repetitive busulfan administration after hematopoietic stem cell gene therapy associated with a dominant HDAC7 clone in a nonhuman primateJianjun Xie
Molecular Hematopoiesis Section, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Hum Gene Ther 21:695-703. 2010....- In vivo selection of hematopoietic progenitor cells and temozolomide dose intensification in rhesus macaques through lentiviral transduction with a drug resistance geneAndre Larochelle
National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Clin Invest 119:1952-63. 2009..Our data therefore suggest that lentivirally mediated gene transfer in transplanted HSCs can provide in vivo chemoprotection of progenitor cells, although selection of long-term repopulating HSCs was not seen... - AMD3100 mobilizes hematopoietic stem cells with long-term repopulating capacity in nonhuman primatesAndre Larochelle
National Heart, Lung, and Blood Institute, National Institutes of Health, Bldg 10 CRC, Rm 4E 5132, 9000 Rockville Pike, Bethesda, MD 20892, USA
Blood 107:3772-8. 2006..Thus, AMD3100-mobilized CD34(+) cells represent an alternative source of hematopoietic stem cells for clinical stem cell transplantation and genetic manipulation with integrating retroviral vectors... - Bone marrow homing and engraftment of human hematopoietic stem and progenitor cells is mediated by a polarized membrane domainAndre Larochelle
Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
Blood 119:1848-55. 2012.... - Ex vivo expansion of retrovirally transduced primate CD34+ cells results in overrepresentation of clones with MDS1/EVI1 insertion sites in the myeloid lineage after transplantationStephanie Sellers
Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892 1290, USA
Mol Ther 18:1633-9. 2010..There was no overrepresentation of MDS1/EVI1 insertions contributing to lymphoid lineages. Strategies involving prolonged ex vivo expansion of transduced cells may increase the risk of genotoxicity... - Sustained high-level polyclonal hematopoietic marking and transgene expression 4 years after autologous transplantation of rhesus macaques with SIV lentiviral vector-transduced CD34+ cellsYoo Jin Kim
Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health NIH, Bethesda, MD 20852, USA
Blood 113:5434-43. 2009.... 
Genetic manipulation of hematopoietic stem cellsAndre Larochelle
Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Semin Hematol 41:257-71. 2004....- HOXB4 and retroviral vectors: adding fuel to the fireAndre Larochelle
Molecular Hematopoiesis Section, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
J Clin Invest 118:1350-3. 2008..These results highlight the risks of combining integrating vectors and growth-promoting genes for clinical applications... - Transduction of rhesus macaque hematopoietic stem and progenitor cells with avian sarcoma and leukosis virus vectorsJingqiong Hu
Molecular Hematopoiesis Section, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Hum Gene Ther 18:691-700. 2007..These findings are encouraging and suggest that this vector system should be explored and further optimized for gene therapy applications targeting hematopoietic stem and progenitor cells... - Transient silencing of PTEN in human CD34(+) cells enhances their proliferative potential and ability to engraft immunodeficient miceInho Kim
Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Exp Hematol 40:84-91. 2012.... - Human and rhesus macaque hematopoietic stem cells cannot be purified based only on SLAM family markersAndre Larochelle
National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
Blood 117:1550-4. 2011..Thus, SLAM family markers do not permit the same degree of HSC enrichment in humans and rhesus macaques as in mice... - Comparison of retroviral transduction efficiency in CD34+ cells derived from bone marrow versus G-CSF-mobilized or G-CSF plus stem cell factor-mobilized peripheral blood in nonhuman primatesPeiman Hematti
Hematology Branch, NHLBI, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
Stem Cells 22:1062-9. 2004..These results demonstrate that steady-state BM is at least equivalent to G-CSF-mobilized peripheral blood as a source of HSCs for retroviral gene transfer and the only currently available source for patients with sickle cell disease... - Intercellular transfer to signalling endosomes regulates an ex vivo bone marrow nicheJennifer M Gillette
Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, Maryland 20892, USA
Nat Cell Biol 11:303-11. 2009..These findings identify a mechanism involving intercellular transfer to signalling endosomes for targeted regulation of signalling and remodelling events within an ex vivo osteoblastic niche... - Hematopoietic stem cell gene therapy: dead or alive?Cole Ferguson
Molecular Hematopoiesis Section, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
Trends Biotechnol 23:589-97. 2005..Scientists are continually revising protocols: going both from "bench to bedside" and, as strikingly demonstrated by HSC gene therapy, from "bedside to bench."..